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https://www.readbyqxmd.com/read/29331529/effect-of-acids-produced-from-carbohydrate-metabolism-in-cryoprotectants-on-the-viability-of-freeze-dried-lactobacillus-and-prediction-of-optimal-initial-cell-concentration
#1
Shumao Cui, Feng Hang, Xiaoming Liu, Zhiyuan Xu, Zhenmin Liu, Jianxin Zhao, Hao Zhang, Wei Chen
For the industrial production of probiotics powder, various sugars have been used as cryoprotectants to preserve probiotics during freeze-drying. Some of these sugars can be metabolized by Lactobacillus with the production of acids during the mix. In this study, we investigated the effect of acids on ATPase, β-galactosidase, lactate dehydrogenase (LDH), integrity and fluidity of cell membrane and the survival rate of Lactobacillus during freeze-drying. In the presence of Lactobacillus, acids were produced from cryoprotectants containing fermentable sugars before freezing, resulting in a decrease in the pH of the bacterial suspension to below 5...
January 11, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29319514/characterization-of-bacteriocin-producing-lactic-acid-bacteria-isolated-from-native-fruits-of-ecuadorian-amazon
#2
Karina Garzón, Clara Ortega, Gabriela N Tenea
Tropical, wild-type fruits are considered biodiverse "hotspots" of microorganisms with possible functional characteristics to be investigated. In this study, several native lactic acid bacteria (LAB) of Ecuadorian Amazon showing highly inhibitory potential were identified and characterized. Based on carbohydrate fermentation profile and 16S rRNA gene sequencing, seven strains were assigned as Lactobacillus plantarum and one strain as Weissella confusa. Using agar-well diffusion method the active synthetized components released in the neutralized and hydroxide peroxide eliminated cell-free supernatant were inhibited by proteolytic enzymes, while the activity was maintained stable after the treatment with catalase, lysozyme, α-amylase and lipase suggesting their proteinaceous nature...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29288050/enhancement-of-immune-response-and-resistance-against-vibrio-parahaemolyticus-in-kuruma-shrimp-marsupenaeus-japonicus-by-dietary-supplementation-of-%C3%AE-1-4-mannobiose
#3
Gehad E Elshopakey, Engy F Risha, Osama A Abdalla, Yo Okamura, Vu Duc Hanh, Masahisa Ibuki, Raja Sudhakaran, Toshiaki Itami
Supplementation of prebiotic carbohydrates can act as a potent immunomodulator and have the efficacy to induce immune-related genes which are involved in host defense. Pure β-1,4-mannobiose (MNB) showed activation of prophenoloxidase of shrimp hemocytes in vitro. The resistance of kuruma shrimp Marsupenaeus japonicus against Vibrio parahaemolyticus was examined after the shrimp were fed with 0 (control), 0.02, 0.2, and 2% MNB supplemented diets. The results showed significantly higher survival rates in MNB supplemented shrimp than those of the control one from 2 - 12 days post challenge...
December 26, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29287787/digestibility-and-nutritional-value-of-fresh-and-stored-pollen-for-honey-bees-apis-mellifera-scutellata
#4
Susan W Nicolson, Susana Da Silva Das Neves, Hannelie Human, Christian W W Pirk
Pollen, the main protein source for honey bees, is mixed with regurgitated nectar or honey during collection and then stored as 'bee bread' before its consumption, mainly by young nurse workers. It has been suggested that storage of pollen improves its nutritional value and digestibility, but there is little evidence for such changes. We fed two fresh pollen types of different protein content (aloe and sunflower), and two stored pollen types (sunflower and a mixed pollen), to young caged worker bees. We measured daily consumption of pollen and sucrose solution, and survival after 14 days...
December 26, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/29279579/stability-of-weakly-acidic-hypochlorous-acid-solution-with-microbicidal-activity
#5
Masayuki Ishihara, Kaoru Murakami, Koichi Fukuda, Shingo Nakamura, Masahiro Kuwabara, Hidemi Hattori, Masanori Fujita, Tomoharu Kiyosawa, Hidetaka Yokoe
 Hypochlorous acid (HOCl) solution (200 ppm, pH 6) was prepared and evaluated for their stabilities and microbicidal activities. We demonstrated that HOCl is unstable against ultraviolet (UV) light, sunshine, contact with air, and elevated temperature (≧25℃). Furthermore, in the HOCl solution, the presence of excess NH2- or CHO-containing organic compounds such as proteins and carbohydrates, or of inorganic ions such as NO2-, SO3-, PO3-, Fe2+, Cu2+, and CuS, resulted in the rapid consumption of HOCl by oxidation reactions, and significantly decreased the microbicidal activity of the HOCl solution against coliform bacteria and total viable cell count...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/29277792/neoadjuvant-chemotherapy-with-gemcitabine-plus-nab-paclitaxel-reduces-the-number-of-cancer-associated-fibroblasts-through-depletion-of-pancreatic-stroma
#6
Tomoharu Miyashita, Hidehiro Tajima, Isamu Makino, Mitsuyoshi Okazaki, Takahisa Yamaguchi, Yoshinao Ohbatake, Sinichi Nakanuma, Hironori Hayashi, Hiroyuki Takamura, Itasu Ninomiya, Sachio Fushida, Koji Kishimoto, John W Harmon, Tetsuo Ohta
BACKGROUND: In this study, the effects of neoadjuvant chemotherapy (NAC) on cancer-associated fibroblasts (CAFs) in pancreatic cancer stroma were investigated. MATERIALS AND METHODS: Density of α-smooth muscle actin (αSMA)-positive fibroblasts in resected surgical specimens from untreated patients, patients receiving conventional gemcitabine plus S-1 (GS), and patients receiving gemcitabine plus nab-paclitaxel (GnP) was determined by hybrid cell counting. 18F-Fluorodeoxyglucose positron-emission tomography (FDG-PET) scans and carbohydrate antigen 19-9 (CA19-9) concentrations were used to assess tumor activity before and after chemotherapy in the GnP group...
January 2018: Anticancer Research
https://www.readbyqxmd.com/read/29223451/mineralization-in-newborn-calves-contributes-to-health-improve-the-antioxidant-system-and-reduces-bacterial-infections
#7
Patrícia Glombowsky, Aleksandro S da Silva, Natan M Soldá, Gabriela M Galli, Angelisa H Biazus, Gabriela Campigotto, Nathieli B Bottari, Rejane S Sousa, Maiara C Brisola, Lenita M Stefani, Matheus D Baldissera, Marta L R Leal, Vera M Morsch, Maria Rosa C Schetinger, Gustavo Machado
The first phase of life of dairy calves has elevated mortality indices linked with low immunity and sanitary challenges, mainly bacterial infections are involved in the pathogenesis of diarrhea, the leading cause of death. Also, other important problem is the nutritional deficiencies, such as the mineral deficiency. Thus, the aim of this study was to evaluate whether an intramuscular mineral supplementation based on selenium, copper, potassium, magnesium and phosphorus possess beneficial effects on health of dairy calves...
December 6, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29221207/chst6-mutation-screening-and-endoplasmatic-reticulum-stress-in-macular-corneal-dystrophy
#8
Liyuan Wang, Xianling Tang, Xiaolin Lv, Encheng Sun, Donglai Wu, Changlin Wang, Ping Liu
Macular corneal dystrophy (MCD) is an autosomal recessive disorder mainly caused by gene mutations of carbohydrate sulfotransferase (CHST6) leading to bilateral visual impairment. Because the mechanism underlying this degeneration remains poorly understood, we investigated molecular alterations and pathways that may be involved in MCD in this issue. Different mutation sites were screened by direct sequencing of the coding region of CHST6. In addition, we described morphological changes in MCD keratocytes by light microscopy and electron microscopy and determined the relationship between the development of this disease and the occurrence of apoptosis through flow cytometry, cell counting kit-8, colony formation assay and other experiments...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29184226/antioxidant-activity-and-protective-effect-of-bee-bread-honey-and-pollen-in-aluminum-induced-anemia-elevation-of-inflammatory-makers-and-hepato-renal-toxicity
#9
Meryem Bakour, Noori S Al-Waili, Nawal El Menyiy, Hamada Imtara, Anna Cristina Figuira, Thia Al-Waili, Badiaa Lyoussi
Aluminum toxicity might be related to oxidative stress, and the antioxidant activity and protective effect of bee bread, which contains pollen, honey and bees' enzymes, on aluminum induced blood and hepato-renal toxicity was investigated in rats. Chemical analysis and antioxidant capacity of bee bread were conducted. The animal experiment in rats included; group 1: received distilled water (10 ml/kg b.wt), group 2: received aluminum chloride (662.2 mg/kg b.wt), group 3: received aluminum chloride (662.2 mg/kg b...
December 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29153877/slowly-biodegradable-organic-compounds-impact-the-biostability-of-non-chlorinated-drinking-water-produced-from-surface-water
#10
W A M Hijnen, R Schurer, J A Bahlman, H A M Ketelaars, R Italiaander, A van der Wal, P W J J van der Wielen
It is possible to distribute drinking water without a disinfectant residual when the treated water is biologically stable. The objective of this study was to determine the impact of easily and slowly biodegradable compounds on the biostability of the drinking water at three full-scale production plants which use the same surface water, and on the regrowth conditions in the related distribution systems. Easily biodegradable compounds in the drinking water were determined with AOC-P17/Nox during 2012-2015. Slowly biodegradable organic compounds measured as particulate and/or high-molecular organic carbon (PHMOC), were monitored at the inlet and after the different treatment stages of the three treatments during the same period...
November 6, 2017: Water Research
https://www.readbyqxmd.com/read/29153522/short-communication-growth-of-dairy-isolates-of-geobacillus-thermoglucosidans-in-skim-milk-depends-on-lactose-degradation-products-supplied-by-anoxybacillus-flavithermus-as-secondary-species
#11
Y Zhao, M Kumar, M P M Caspers, M N Nierop Groot, J M B M van der Vossen, T Abee
Thermophilic bacilli such as Anoxybacillus and Geobacillus are important contaminants in dairy powder products. Remarkably, one of the common contaminants, Geobacillus thermoglucosidans, showed poor growth in skim milk, whereas significant growth of G. thermoglucosidans was observed in the presence of an Anoxybacillus flavithermus dairy isolate. In the present study, we investigated the underlying reason for this growth dependence of G. thermoglucosidans. Whole-genome sequences of 4 A. flavithermus strains and 4 G...
November 15, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29136334/identification-of-plasma-membrane-glycoproteins-specific-to-human-glioblastoma-multiforme-cells-using-lectin-arrays-and-lc-ms-ms
#12
YaeEun Park, Jeonghun Yeom, YoungSoo Kim, Hye Jin Lee, Ki-Cheol Han, SeungTaek Lee, Cheolju Lee, Ji Eun Lee
Glioblastoma, also known as glioblastoma multiforme (GBM), is the most malignant type of brain cancer and has poor prognosis with a median survival of less than one year. While the structural changes of tumor cell surface carbohydrates are known to be associated with invasive behavior of tumor cells, the cell surface glycoproteins to differentiate the low- and high-grade glioma cells can be potential diagnostic markers and therapeutic targets for GBMs. In the present study, lectin arrays consisting of eight lectins were employed to explore cell surface carbohydrate expression patterns on low-grade oligodendroglioma cells (Hs683) and GBM cells (T98G)...
November 14, 2017: Proteomics
https://www.readbyqxmd.com/read/29132740/effects-of-using-symbiotics-in-the-clinical-nutritional-evolution-of-patients-with-chronic-pancreatitis-study-prospective-randomized-controlled-double-blind
#13
Patricia Quadros Dos Santos, Jorge C Guedes, Rosangela P de Jesus, Raquel Rocha Dos Santos, Rosemeiri L Fiaconne
Patients with chronic pancreatitis (CP) present malabsorption and changes in nutritional status. In this prospective, randomized, controlled, double-blinded, intervention study, developed at the Clinic of Pancreas, we aimed to assess whether the use of symbiotics changes the nutritional status, the biochemical data and the intestinal rate of these patients. The intervention consisted of administering 12 g/day of symbiotics composed of Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus acidophilus, Bifidobacterium bifidum and fructooligosaccharides to the intervention group and 12 g/day of medium absorption complex carbohydrate to the control group...
April 2017: Clinical Nutrition ESPEN
https://www.readbyqxmd.com/read/29098834/-transcriptomics-of-therapeutic-effect-of-huangqi-liuyi-decoction-in-treating-type-2-diabetes
#14
Yue Chang, Jiao Xu, Song Yan, Zhen-Peng Liu, Wei-Chao Ren, Kai-Xue Zhang, Wei Ma, Xiu-Bo Liu
In this study, Illumina sequencing platform was applied in sequencing rat pancreas, counting expression of target points, analyzing expression differences among blank group, model group and Huangqi Liuyi decoction group and exploring the therapeutic effect and mechanism of Huangqi Liuyi decoction on type 2 diabetes mellitus. According to the result, 24.25% of these genes belonged to the unknown functional class, which was the largest classification unit according to the classification analysis of genes by eggNOG...
July 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29098543/effect-of-chromium-nanoparticles-on-physiological-stress-induced-by-exogenous-dexamethasone-in-japanese-quails
#15
Atefeh Berenjian, Seyed Davood Sharifi, Abdollah Mohammadi-Sangcheshmeh, Shekofeh Ghazanfari
Chromium (Cr), as an essential trace element, plays a critical role in carbohydrate, protein, and lipid metabolism in animals. It has been suggested that the beneficial effects of Cr increase by environmental stresses, which lead to greater Cr effects on stressed animals. The objective of the present study was to evaluate the response of physiologically stressed Japanese quails (Coturnix japonica) to dietary chromium nanoparticle (Nano-Cr) supplementation. The stress was induced by adding dexamethasone (0.6 mg/kg BW) to Japanese quails' diet from 17 to 22 days of age...
November 2, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/29097636/antiplasmodial-efficacy-of-calotropis-gigantea-l-against-plasmodium-falciparum-3d7-strain-and-plasmodium-berghei-anka
#16
P V V Satish, D Santha Kumari, K Sunita
BACKGROUND & OBJECTIVES: Malaria is a deadly parasitic disease, having a high rate of incidence and mortality across the world. The spread and development of resistance against chemical insecticides is one of the major problems associated with malaria treatment and control. Hence, plant based formulations may serve as an alternative source towards development of new drugs for treatment of malaria. The present study was aimed to evaluate the in vitro antiplasmodial activities of leaf, stem and flower of Calotropis gigantea against chloroquine-sensitive Plasmodium falciparum (3D7 strain) and its cytotoxicity against THP-1 cell lines...
July 2017: Journal of Vector Borne Diseases
https://www.readbyqxmd.com/read/29073288/complementary-and-alternative-medicine-in-brazilian-children-and-adolescents-with-type-1-diabetes-mellitus
#17
Luciana Carla Bezerra Machado, Crésio Alves
INTRODUCTION: Patients with type 1 diabetes mellitus (T1DM) resort to complementary and alternative medicine (CAM) for symptomatic relief, to inhibit progression or to reduce side effects associated with conventional therapy. OBJECTIVES: Build a profile and assess the prevalence of the use of CAM in Brazilian children and adolescents with T1DM under conventional treatment. METHODS: Cross-sectional study with interviews with the legal representatives of patients with T1DM (of both gender, aged 2- 17 years)...
2017: Pediatric Endocrinology, Diabetes, and Metabolism
https://www.readbyqxmd.com/read/29028442/the-use-of-mobile-applications-among-adults-with-type-1-and-type-2-diabetes-results-from-the-second-miles-australia-miles-2-study
#18
Steven Trawley, Shaira Baptista, Jessica L Browne, Frans Pouwer, Jane Speight
BACKGROUND: While the number of diabetes-specific mobile applications (apps) continues to grow, there is a lack of knowledge about their actual use. METHODS: The second MILES (Management and Impact for Long-term Empowerment and Success)-Australia study was a national cross-sectional survey of the psychological, behavioral, and social aspects of diabetes for adults with type 1 diabetes (T1D) and type 2 diabetes (T2D). Associations between diabetes-specific app usage and demographic, clinical, and psychosocial variables were examined...
October 13, 2017: Diabetes Technology & Therapeutics
https://www.readbyqxmd.com/read/29025077/impact-of-fasting-on-complete-blood-count-assayed-in-capillary-blood-samples
#19
Barbara K Koscielniak, Aleksandra Charchut, Magdalena Wójcik, Krystyna Sztefko, Przemyslaw J Tomasik
Background: To determine the effect of food intake on complete blood count (CBC) results. Method: We analyzed the CBC in whole capillary blood specimens collected from 33 adult volunteers into microtubes between 8:00 AM and 11:00 AM. Blood specimens were drawn from patients who had been fasting 12 hours and then 1 and 2 hours after consumption of a light meal containing carbohydrates, protein, and lipids. All measurements were performed using the hematologic analyzer Sysmex XN-1000...
November 8, 2017: Laboratory Medicine
https://www.readbyqxmd.com/read/29020671/oral-lactobacillus-counts-predict-weight-gain-susceptibility-a-6-year-follow-up-study
#20
Johanne Aviaja Rosing, Karen Christina Walker, Benjamin A H Jensen, Berit L Heitmann
BACKGROUND: Recent studies have shown an association between weight change and the makeup of the intestinal microbiota in humans. Specifically, Lactobacillus, a part of the entire gastrointestinal tract's microbiota, has been shown to contribute to weight regulation. AIM: We examined the association between the level of oral Lactobacillus and the subsequent 6-year weight change in a healthy population of 322 Danish adults aged 35-65 years at baseline. DESIGN: Prospective observational study...
2017: Obesity Facts
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