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https://www.readbyqxmd.com/read/27899642/the-ucsc-genome-browser-database-2017-update
#1
Cath Tyner, Galt P Barber, Jonathan Casper, Hiram Clawson, Mark Diekhans, Christopher Eisenhart, Clayton M Fischer, David Gibson, Jairo Navarro Gonzalez, Luvina Guruvadoo, Maximilian Haeussler, Steve Heitner, Angie S Hinrichs, Donna Karolchik, Brian T Lee, Christopher M Lee, Parisa Nejad, Brian J Raney, Kate R Rosenbloom, Matthew L Speir, Chris Villarreal, John Vivian, Ann S Zweig, David Haussler, Robert M Kuhn, W James Kent
Since its 2001 debut, the University of California, Santa Cruz (UCSC) Genome Browser (http://genome.ucsc.edu/) team has provided continuous support to the international genomics and biomedical communities through a web-based, open source platform designed for the fast, scalable display of sequence alignments and annotations landscaped against a vast collection of quality reference genome assemblies. The browser's publicly accessible databases are the backbone of a rich, integrated bioinformatics tool suite that includes a graphical interface for data queries and downloads, alignment programs, command-line utilities and more...
November 29, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27895110/nucleosome-occupancy-as-a-novel-chromatin-parameter-for-replication-origin-functions
#2
Jairo Rodriguez, Laura Lee, Bryony Lynch, Toshio Tsukiyama
Eukaryotic DNA replication initiates from multiple discrete sites in the genome termed origins of replication (origins). Prior to S phase, multiple origins are poised to initiate replication by recruitment of the pre-replicative complex (pre-RC). For proper replication to occur, origin activation must be tightly regulated. At the population level, each origin has a distinct firing time and frequency of activation within S phase. Many studies have showed that chromatin can strongly influence initiation of DNA replication...
November 28, 2016: Genome Research
https://www.readbyqxmd.com/read/27894855/galloylquinic-acid-derivatives-from-copaifera-langsdorffii-leaves-display-gastroprotective-activity
#3
Erick V S Motta, Marivane Lemos, Juliana C Costa, Vilmar C Banderó-Filho, Astrid Sasse, Helen Sheridan, Jairo K Bastos
Nine new methylated galloylquinic acids were isolated from an aqueous fraction of Copaifera langsdorffii (Fabaceae-Caesalpinioideae) leaf hydroalcoholic extract (3-8, 11, 12, and 14), along with three known methylated galloylquinic acids (1, 2, and 15) and four galloylquinic acids (9, 10, 13, and 16). These compounds were characterized by nuclear magnetic resonance spectroscopy and mass spectrometry. They were further tested in a gastroprotection assay (Ethanol-HCl induced ulcer model in mice), in which all of them significantly reduced the total lesion area, and increased the cure ratio in comparison with pantoprazole...
November 25, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27890876/synthesis-and-antifungal-in-vitro-evaluation-of-pyrazolo-3-4-b-pyridines-derivatives-obtained-by-aza-diels-alder-reaction-and-microwave-irradiation
#4
Jairo Quiroga, Yazmín Villareal, Jaime Gálvez, Alejandro Ortíz, Braulio Insuasty, Rodrigo Abonia, Marcela Raimondi, Susana Zacchino
A series of pyrazolo[3,4-b]pyridines were prepared by a microwawe-assisted aza-Diels-Alder reaction between pyrazolylformimidamides 1 and β-nitrostyrenes 2 in toluene as the solvent. This procedure provides a simple one-step and environmentally friendly methodology with good yields for the synthesis of these compounds. All compounds were tested for antifungal activity against two clinically important fungi Candida albicans and Cryptococcus neoformans. Within the compounds of the series bearing a -CH3 group on the carbon C-3 of the azole ring (3a-3e), the compound without a substituent on the p'-phenyl ring (3a), showed the best activity against both fungi, followed by the p'-Br-phenyl (3c)...
November 25, 2016: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27890081/association-between-leishmania-infantum-dna-in-the-hair-of-dogs-and-their-infectiousness-to-lutzomyia-longipalpis
#5
Rafaela de Sousa Gonçalves, Carlos R Franke, Jairo T Magalhães-Junior, Bárbara M P S Souza, Manuela S Solcà, Daniela F Larangeira, Stella Maria Barrouin-Melo
Diagnosis of infection with Leishmania infantum by DNA detection in the hair has been recently demonstrated in dogs and wild animals. Our objective was to investigate if polymerase chain reaction (PCR) in hair might be used to identify infectious dogs. Thus, we assessed the infectiousness to Lutzomyia longipalpis by xenodiagnosis in comparison with the detection of L. infantum DNA by PCR in the hair, and with serology for anti-Leishmania IgG by ELISA in 15 positive dogs for L. infantum infection. Eight healthy dogs were included as negative controls...
December 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27865418/characterizing-emergence-and-divergence-in-the-first-follicular-wave-in-a-tropically-adapted-bos-taurus-breed
#6
Rodrigo Dorneles Tortorella, Guilherme de Paula Nogueira, Mairon Rocha Modesto, Priscilla Cristine Passoni Silva, Joseane Padilha da Silva, Jairo Pereira Neves, Alexandre Floriani Ramos
Tropically adapted Bos taurus breeds are descended from the cattle which were brought from the Iberian Peninsula to Latin America by the colonizers and which adapted over time to local conditions. These breeds have become a genetic treasure that must be preserved. The objective of this study was to characterize ovarian follicular emergence and divergence in tropically adapted B. taurus cows and heifers. Cyclic heifers (n = 11) and nonlactating, multiparous cows (n = 11) were examined at 8-hour intervals using ultrasonography starting on the day following the final application of PGF2α, which was used to synchronize the estrous cycle, and ending 5 days after ovulation...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27863951/detection-of-helicobacter-pylori-and-the-genotypes-of-resistance-to-clarithromycin-and-the-heterogeneous-genotype-to-this-antibiotic-in-biopsies-obtained-from-symptomatic-children
#7
John Jairo Aguilera-Correa, Pedro Urruzuno, Josefa Barrio, María José Martinez, Sonia Agudo, Angela Somodevilla, Laura Llorca, Teresa Alarcón
: The aim of this study was to use a commercially available kit (GenoType® HelicoDR; Hain Life Science, Germany) to detect Helicobacter pylori infection and clarithromycin resistance genotype in biopsies obtained from symptomatic children. RESULTS: 111 out of 136 (81.6%) biopsies were H. pylori positive by genotype: 47 (42.3%) showed wild-type genotype, 53 resistant genotype (47.7%) and 11 heterogeneous genotype (9.9%). Culture was negative in 27 out of the 111 genotyped biopsies...
March 2, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27863315/positive-effects-of-transcranial-direct-current-stimulation-in-adult-patients-with-attention-deficit-hyperactivity-disorder-a-pilot-randomized-controlled-study
#8
Carolina Tosetto Cachoeira, Douglas Teixeira Leffa, Suzana Doneda Mittelstadt, Lorenna Sena Teixeira Mendes, Andre R Brunoni, Jairo Vinicius Pinto, Vítor Blazius, Vitoria Machado, Claiton Henrique Dotto Bau, Luis Augusto Rohde, Eugenio Horacio Grevet, Pedro Schestatsky
Almost 30% of adult patients with attention-deficit/hyperactivity disorder (ADHD) do not respond or tolerate standard pharmacological interventions. Few clinical investigations addressed the efficacy and tolerability of transcranial direct current stimulation (tDCS), a non-invasive neuromodulatory technique, in the disorder. We performed a double-blind, sham-controlled randomized clinical trial in 17 patients with ADHD. The set up for tDCS was the following: 2mA/20min/day for 5 days with the anode over the right dorsolateral prefrontal cortex and cathode over the left dorsolateral prefrontal cortex...
November 10, 2016: Psychiatry Research
https://www.readbyqxmd.com/read/27829185/weathering-processes-and-dating-of-soil-profiles-from-s%C3%A3-o-paulo-state-brazil-by-u-isotopes-disequilibria
#9
Daniel Marcos Bonotto, Jairo Roberto Jiménez-Rueda, Isabella Cruz Fagundes, Carlos Roberto Alves Fonseca Filho
This study reports the use of the U-series radionuclides (238)U and (234)U for dating two soil profiles. The soil horizons developed over sandstones from Tatuí and Pirambóia formations at the Paraná sedimentary basin, São Paulo State, Brazil. Chemical data in conjunction with the (234)U/(238)U activity ratios (AR's) of the soil horizons allowed investigating the U-isotopes mobility in the shallow oxidizing environment. Kaolinization and laterization processes are taking place in the profiles sampled, as they are especially common in regions characterized by a wet and dry tropical climate and a water table that is close to the surface...
January 2017: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/27828572/-the-association-between-common-mental-disorders-and-subjective-health-conditions-among-the-elderly
#10
Andréa Maria Eleutério de Barros Lima Martins, Jairo Evangelista Nascimento, João Gabriel Silva Souza, Maria Aparecida Barbosa de Sá, Sara de Barros Lima Feres, Bruno Porto Soares, Efigenia Ferreira E Ferreira
The scope of this study was to evaluate the association between the presence of common mental disorders and the impairment of subjective health conditions among the elderly. It involved a cross-sectional analytical survey conducted among all the elderly residents in the urban area of a Brazilian municipality with low population density. Mental disorders were evaluated using the short version of the Goldberg General Health questionnaire. Subjective and normative health conditions were evaluated and logistic regression was applied (OR/CI95%) with a 5% significance level...
November 2016: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/27821892/afrocolombian-ethnicity-a-paradoxical-protective-factor-against-dengue
#11
Jorge Humberto Rojas Palacios, Alberto Alzate, Héctor Jairo Martínez Romero, Alberto Ignacio Concha-Eastman
INTRODUCTION: Dengue is a priority public health problem. During epidemics in Cuba and Haiti, ethnic African descendant population had lower risk of dengue, and the ethnic factor was proposed as a protective one. OBJECTIVE: To determine the relation between the Dengue's cumulative incidence and the Afro-Colombian proportion in communities of Cali, during the epidemic of 2013. METHODS: This study was conducted in Cali, Colombia. The design was ecological, using information from the National Census 2005 projected to 2013, from the National Administrative Department of Statistics (DANE), and the National Epidemiological Surveillance System...
September 30, 2016: Colombia Médica: CM
https://www.readbyqxmd.com/read/27821562/oxidative-stress-in-granulosa-lutein-cells-from-in-vitro-fertilization-patients
#12
Julio Ávila, Rebeca González-Fernández, Deborah Rotoli, Jairo Hernández, Angela Palumbo
Ovarian aging is associated with gradual follicular loss by atresia/apoptosis. Increased production of toxic metabolites such as reactive oxygen species (ROS) and reactive nitrogen species as well as external oxidant agents plays an important role in the process of ovarian senescence and in the pathogenesis of ovarian pathologies such as endometriosis and polycystic ovary syndrome (PCOS). This review provides a synthesis of available studies of oxidative stress (OS) in the ovary, focusing on the most recent evidence obtained in mural granulosa-lutein (GL) cells of in vitro fertilization patients...
December 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27810920/in-vivo-conditional-deletion-of-hdac7-reveals-its-requirement-to-establish-proper-b-lymphocyte-identity-and-development
#13
Alba Azagra, Lidia Román-González, Olga Collazo, Javier Rodríguez-Ubreva, Virginia G de Yébenes, Bruna Barneda-Zahonero, Jairo Rodríguez, Manuel Castro de Moura, Joaquim Grego-Bessa, Irene Fernández-Duran, Abul B M M K Islam, Manel Esteller, Almudena R Ramiro, Esteban Ballestar, Maribel Parra
Class IIa histone deacetylase (HDAC) subfamily members are tissue-specific gene repressors with crucial roles in development and differentiation processes. A prominent example is HDAC7, a class IIa HDAC that shows a lymphoid-specific expression pattern within the hematopoietic system. In this study, we explored its potential role in B cell development by generating a conditional knockout mouse model. Our study demonstrates for the first time that HDAC7 deletion dramatically blocks early B cell development and gives rise to a severe lymphopenia in peripheral organs, while also leading to pro-B cell lineage promiscuity...
November 14, 2016: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/27809965/parasite-load-in-the-blood-and-skin-of-dogs-naturally-infected-by-leishmania-infantum-is-correlated-with-their-capacity-to-infect-sand-fly-vectors
#14
Lairton Souza Borja, Orlando Marcos Farias de Sousa, Manuela da Silva Solcà, Leila Andrade Bastos, Marcelo Bordoni, Jairo Torres Magalhães, Daniela Farias Larangeira, Stella Maria Barrouin-Melo, Deborah Bittencourt Mothé Fraga, Patrícia Sampaio Tavares Veras
The sand fly Lutzomyia longipalpis is primarily responsible for the transmission of visceral leishmaniasis (VL) in the New World, and dogs are considered to be the main urban reservoir of this disease. In order to improve the efficacy of control measures, it is essential to assess the transmission capacity of Leishmania infantum to the sand fly vector by naturally infected dogs. The present study investigated the existence of correlations between canine clinical presentation and the intensity of parasite load in the blood, skin and spleen of naturally infected dogs...
October 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27795670/analgesic-effect-of-dexamethasone-after-arthroscopic-knee-surgery-a-randomized-controlled-trial
#15
Jairo Moyano, Maria García, Maria Caicedo
Background. Dexamethasone is sometimes used as a coanalgesic because of its anti-inflammatory properties. Objective. To evaluate opioid use, postoperative pain intensity, and side effects after a single dose of dexamethasone in patients undergoing arthroscopic knee surgery. Methods. In this randomized controlled study patients were randomized to receive either 10 mg of intravenous dexamethasone (DM group) or 0.9% normal saline (NS group) during the intraoperative period. Primary outcomes were pain intensity and total morphine and codeine use after surgery...
2016: Pain Research & Management: the Journal of the Canadian Pain Society
https://www.readbyqxmd.com/read/27787258/design-and-performance-of-boreas-the-beamline-for-resonant-x-ray-absorption-and-scattering-experiments-at-the-alba-synchrotron-light-source
#16
Alessandro Barla, Josep Nicolás, Daniele Cocco, Secundino Manuel Valvidares, Javier Herrero-Martín, Pierluigi Gargiani, Jairo Moldes, Claude Ruget, Eric Pellegrin, Salvador Ferrer
The optical design of the BOREAS beamline operating at the ALBA synchrotron radiation facility is described. BOREAS is dedicated to resonant X-ray absorption and scattering experiments using soft X-rays, in an unusually extended photon energy range from 80 to above 4000 eV, and with full polarization control. Its optical scheme includes a fixed-included-angle, variable-line-spacing grating monochromator and a pair of refocusing mirrors, equipped with benders, in a Kirkpatrick-Baez arrangement. It is equipped with two end-stations, one for X-ray magnetic circular dichroism and the other for resonant magnetic scattering...
November 1, 2016: Journal of Synchrotron Radiation
https://www.readbyqxmd.com/read/27773600/the-physician-patient-working-alliance-theory-research-and-future-possibilities
#17
Jairo N Fuertes, Arielle Toporovsky, Mariela Reyes, Jennifer Bennett Osborne
OBJECTIVE: This article discusses the physician-patient working alliance and reviews the empirical research that has been generated on the working alliance to date. METHODS: The paper presents a brief history of the study of the physician-patient relationship, and discusses constructs that have examined aspects of the relationship, such as empathy, trust, and shared decision-making. Lastly, a meta-analysis was conducted based on the seven empirical studies (a total N of 1023 patients) that have examined the physician-patient working alliance...
October 19, 2016: Patient Education and Counseling
https://www.readbyqxmd.com/read/27757946/facile-synthesis-of-monodisperse-gold-nanocrystals-using-virola-oleifera
#18
Bárbara A Milaneze, Jairo P Oliveira, Ingrid Augusto, Wanderson J Keijok, Andressa S Côrrea, Débora M Ferreira, Otalíbio C Nunes, Rita de Cássia R Gonçalves, Rodrigo R Kitagawa, Vinícius G Celante, André Romero da Silva, Ana Claudia H Pereira, Denise C Endringer, Ricardo P Schuenck, Marco C C Guimarães
The development of new routes and strategies for nanotechnology applications that only employ green synthesis has inspired investigators to devise natural systems. Among these systems, the synthesis of gold nanoparticles using plant extracts has been actively developed as an alternative, efficient, cost-effective, and environmentally safe method for producing nanoparticles, and this approach is also suitable for large-scale synthesis. This study reports reproducible and completely natural gold nanocrystals that were synthesized using Virola oleifera extract...
December 2016: Nanoscale Research Letters
https://www.readbyqxmd.com/read/27743973/the-influence-of-angiostrongylus-cantonensis-nematoda-metastrongylidae-infection-on-the-aerobic-metabolism-of-biomphalaria-straminea-and-biomphalaria-tenagophila-mollusca-gastropoda
#19
Mariana G Lima, Vinícius M Tunholi-Alves, Fabrício N Gaudêncio, Florence G Martins, Rosane N Castro, Silvana C Thiengo, Juberlan S Garcia, Arnaldo Maldonado, Jairo Pinheiro
Angiostrongylus cantonensis is considered the main agent responsible for human eosinophilic meningoencephalitis. This parasite has low specificity for mollusk hosts and it can also use aquatic snails as auxiliary hosts. Studies based on the metabolic profile of Biomphalaria spp. infected by A. cantonensis have been conducted to observe parasite-host interactions. In the present study, the glucose content in the hemolymph and glycogen content in the digestive gland and cephalopedal mass of Biomphalaria tenagophila and Biomphalaria straminea experimentally infected by A...
October 12, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27740646/the-effect-of-osteopontin-and-an-osteopontin-derived-synthetic-peptide-coating-on-osseointegration-of-implants-in-a-canine-model
#20
Joseph P Fiorellini, Sven Glindmann, Jairo Salcedo, Hans-Peter Weber, Chang-Joo Park, Hector L Sarmiento
Osteopontin (OPN) and an OPN-derived synthetic peptide, OC-1016, have demonstrated their potential to enhance osseointegration in vitro. The purpose of this study was to evaluate bone-to-implant contact (BIC) and surrounding bone density (BD) of implants coated with either recombinant human OPN (rhOPN) or OC-1016 as compared with noncoated titanium plasma sprayed (TPS) surface in a canine model. Histomorphometric analysis revealed that at 4 weeks, %BIC and %BD of coated implants were significantly higher than those of noncoated TPS implants...
November 2016: International Journal of Periodontics & Restorative Dentistry
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