Rrrr

The autotetraploid Carassius auratus (4nRR, 4 n =200, RRRR) is derived from whole-genome duplication of Carassius auratus red var. (RCC, 2 n =100, RR). In the current study, we demonstrated that chromatophores and pigment changes directly caused the coloration and variation of 4nRR skin (red in RCC, brownish-yellow in 4nRR). To further explore the molecular mechanisms underlying coloration formation and variation in 4nRR, we performed transcriptome profiling and molecular functional verification in RCC and 4nRR...

Transcriptomics analyses play pivotal roles in understanding the complex regulatory networks that govern cellular processes. The abundance of rRNAs, which account for 80-90% of total RNA in eukaryotes, limits the detection and investigation of other transcripts. While mRNAs and long non-coding RNAs have polyA(+) tails that are often used for positive selection, investigations of polyA(-) RNAs, such as circular RNAs, histone mRNAs, and small RNAs, typically require the removal of the abundant rRNAs for enrichment...

Propylene polymerizations with different ketimide-modified half-titanocene catalysts, Cp'TiCl2 (N=C t Bu2 ) [Cp' = C5 H5 ( 1 ), C5 Me5 ( 2 ), Me3 SiC5 H4 ( 3 )], with MAO as a cocatalyst, were investigated. The obtained polymers were studied in detail by determining their microstructure, molar masses, thermal, and mechanical properties. The Cp*-ketimide, (C5 Me5 )TiCl2 (N=C t Bu2 ) ( 2 ), exhibited higher catalytic activities than Cp'TiCl2 (N=C t Bu2 ) ( 1 , 3 ), yielding higher molar mass polymers, M w up to 1400 Kg/mol...

Herein, we report a concise, effective and scalable strategy that the introduction of polar molecules (PMs) (e.g., anisole (PhOMe), phenetole (PhOEt), 2-methoxynaphthalene (NaphOMe), thioanisole (PhSMe), and N,N-dimethylaniline (PhNMe2 )) as continuously coordinated neutral ligand of cationic active species in-situ generated from the CGC-type rare-earth metal complexes A-F/Ali Bu3 /[Ph3 C][B(C6 F5 )4 ] ternary systems can easily switch the regio- and stereoselectivity of the polymerization of conjugated dienes (CDs, including 2-subsituted CDs such as isoprene (IP) and myrcene (MY), 1,2-disubstituted CD ocimene (OC), and 1-substituted polar CD 1-(para-methoxyphenyl)-1,3-butadiene (p-MOPB)) from poor selectivities to high selectivities (for IP and MY: 3,4-selectivity up to 99%; for OC: trans-1,2-selectivity up to 93% (mm up to 90%); for p-MOPB: 3,4-syndioselectivity (3,4- up to 99%, rrrr up to 96%))...

Oxidation and protein phosphorylation are critical mechanisms involved in regulating various cellular activities. Increasing research has suggested that oxidative stress could affect the activities of specific kinases or phosphatases, leading to alterations in the phosphorylation status of certain proteins. Ultimately, these alterations can affect cellular signaling pathways and gene expression patterns. However, the relationship between oxidation and protein phosphorylation remains complex and not yet fully understood...

Established autotetraploids often have a highly stable meiosis with high fertility compared with neo-autotetraploids. The autotetraploid Carassius auratus (4n = 200, RRRR) (4nRR), which stemmed from whole-genome duplication of Carassius auratus red var. (2n = 100, RR) (RCC), produces diploid gametes with an adopted diploid-like chromosome pairing in meiosis and maintains the formation of autotetraploid lineages. In this study, we focused on Dmc1 , a meiosis-specific recombinase during the prophase of meiosis I, and elaborated on the genetic variation, alternative transcription, expression characterization, and epigenetic modification of Dmc1 in RCC and 4nRR...

Micro-vision possesses high in-focus-plane motion tracking accuracy. Unfortunately, out-of-focus-plane displacements cannot be avoided, decreasing the in-focus-plane tracking accuracy of micro-vision. In this paper, a spatial nanopositioner is proposed to evaluate the out-of-focus-plane performance of a micro-vision system. A piezoelectric-actuated spatial multi-degree-of-freedom (multi-DOF) nanopositioner is introduced. Three in-plane Revolute-Revolute-Revolute-Revolute (RRRR) compliant parallel branched chains produce in-focus-plane motions...

INTRODUCTION: Robotic abdominal wall reconstruction (RAWR) is one of the most significant advances in the management of complex abdominal wall hernias. The objective of this study was to evaluate long term outcomes in a cohort of patients that underwent complex RAWR in a single center. METHODS: This was a longitudinal retrospective review of a cohort of 56 patients who underwent complex RAWR at least 24 months prior by a single surgeon at a tertiary care institution...

Although the strategy in cancer vaccination is to provide a therapeutic effect against an established tumor, there is an urgent need to develop prophylactic vaccines for non-viral cancers. In this study, we prepared polyplex nanoparticles through electrostatic interactions between a positively-charged modified tumor associated antigen, namely human derived melanoma gp10025-33 peptide (KVPRNQDWL-RRRR), and a negatively charged cytosine-phosphate-guanosine motif (CpG-ODN) adjuvant. We previously demonstrated successful transdermal delivery of various hydrophilic macromolecules by iontophoresis (IP) using weak electricity...

The development of efficient catalysts for the copolymerization of nonpolar monomers with polar monomers remains a great challenging task in polymer synthesis. A one-pot reaction of anhydrous LnCl3 with pyridyl-methylene-functionalized octamethylfluorenyl lithium OctFlu-CH2 PyLi in a 1:1 molar ratio, followed by alkylation with 2 equiv of LiCH2 SiMe3 in THF afforded the fluorenyl-ligated rare-earth metal bis(alkyl) complexes (OctFlu-CH2 Py)Ln(CH2 SiMe3 )2 (THF) [Ln = Sc (1), Y (2)]. Both complexes were isolated as neutral species and were characterized by NMR spectrum and elemental analysis...

Piezoelectric actuators (PEAs) and compliant parallel mechanisms (CPMs) are advantageous for designing nanopositioning stages (NPSs) with multiple degrees of freedom (multi-DOFs). This paper proposes a new NPS that uses PEAs and CPMs with multiple spatial DOFs. First, the design of the mechanism is introduced. Six parallel kinematics revolute-revolute-revolute-revolute (RRRR) branched chains were used to create a 6-RRRR CPM for superior mechanical performance. Three in-plane and three out-of-plane chains were combined using a two-in-one structure to ensure fabrication feasibility...

A series of first-row early transition metal dialkyl complexes bearing pincer ligands [(POCOP)M(CH2 SiMe3 )2 ] (POCOP: (2,6-( t Bu2 PO)2 -C6 H3 ); 1-Sc: M = Sc; 1-Ti: M = Ti; 1-V: M = V) and [(PCP)M(CH2 SiMe3 )2 ] (PCP: (2,6-( t Bu2 PCH2 )2 -C6 H3 ); 2-Sc: M = Sc; 2-Ti: M = Ti) have been synthesized. These dialkyl complexes were characterized by single-crystal X-ray diffraction, NMR spectroscopy, and solution magnetic susceptibility (Evans method) analyses appropriately. All the complexes exhibited square pyramidal geometries with different extents of distortion...

This study aimed to explore the effect of induced electric field (IEF) treatment on acid hydrolysis of corn starch by altering the connection modes of sample coils of a 4-reactor IEF system. Results suggested that IEF treatment could enhance the hydrolysis of corn starch and series connection (1. RRRR, η=16ESi 2 Pin 4ZSi +Zload ) exhibited higher energy efficiency than parallel (9. (RRRR), η=4ESi 2 Pin ZSi +4Zload ), thus contributing to more extensive hydrolysis. Although no new functional group was formed, the starch granules were partially cracked into pieces and the crystallinity was slightly increased after IEF-assisted hydrolysis...

A simple and facile synthetic pathway for accessing new derivatizable bulky-demanding octahydrofluorenyl (OHF) ligands has been developed, and a series of half-sandwich rare-earth metal (Sc, Y, Lu) complexes bearing the OHF ancillary ligands have been synthesized. In conjunction with a borate, the OHF-ligated Sc complexes exhibited high catalytic activity for styrene (co)polymerization to afford polymers with highly syndiotactic polystyrene sequence (>99% rrrr ).

BACKGROUND: Autotetraploid Carassius auratus (4n = 200, RRRR) (abbreviated as 4nRR) is derived from whole genome duplication of Carassius auratus red var. (2n = 100, RR) (abbreviated as RCC). Ribosome DNA (rDNA) is often used to study molecular evolution of repeated sequences because it has high copy number and special conserved coding regions in genomes. In this study, we analysed the sequences (5S, ITS1-5.8S-ITS2 region), structure, methylation level (NTS and IGS), and expression level (5S and 18S) of 5S and 45S ribosomal RNA (rRNA) genes in 4nRR and RCC in order to elucidate the effects of autotetraploidization on rDNA in fish...

Design and synthesis of air-stable and easily tailored high-performance single-molecule magnets (SMMs) are of great significance toward the implementation of SMMs in molecular-based magneto-electronic devices. Here, by introducing electron-withdrawing fluorinated substituents on equatorial ligand, two chiral Dy(III) macrocyclic complexes, RRRR -Dy- D 6h F12 ( 1 ) and SSSS -Dy- D 6h F12 ( 2 ), with a record anisotropy barrier exceeding 1800 K and the longest relaxation time approaching 2500 s at 2.0 K for all known air-stable SMMs, were obtained...

Importance: Electronic health records (EHRs) often include default alerts that can influence physician selection of antibiotics, which in turn may be associated with a suboptimal choice of agents and increased antibiotic resistance. Objective: To examine whether removal of a default alert in the EHR to avoid cephalosporin use in patients with penicillin allergies is associated with changes in cephalosporin dispensing or administration in these patients. Design, Setting, and Participants: This retrospective cohort study of a natural experiment included data on patients who had received antibiotic treatment in the hospital or outpatient setting in 2 regions of a large, integrated health system in California from January 1, 2017, to December 31, 2018...

To date, surface plasmon resonance (SPR) biosensors have been exploited in numerous different contexts while continuously pushing boundaries in terms of improved sensitivity, specificity, portability and reusability. The latter has attracted attention as a viable alternative to disposable biosensors, also offering prospects for rapid screening of biomolecules or biomolecular interactions. In this context here, we developed an approach to successfully regenerate a fiber-optic (FO)-SPR surface when utilizing cobalt (II)-nitrilotriacetic acid (NTA) surface chemistry...

Entry of circovirus into the host cell nucleus is essential for viral replication during the early stage of infection. However, the mechanisms by which nucleolar shuttle proteins are used during viral replication is still not well understood. Here, we report a previously unidentified nucleolar localization signal in circovirus capsid protein (Cap), and that circovirus hijacks the nucleolar phosphoprotein nucleophosmin-1 (NPM1) to facilitate its replication. Colocalization analysis showed that NPM1 translocates from the nucleolus to the nucleoplasm and cytoplasm during viral infection...

Three new tetranuclear europium(III) alternating circular helicates, ( 2- Ph RRRR/ 2- Ph SSSS ) [( R )- or ( S )-Ph-Pybox]4 EuIII 4 (BPP)6 , ( 2- iPr RRRR / 2- iPr SSSS ) [( R )- or ( S )-i Pr-Pybox]4 EuIII 4 (BPP)6 , and ( 3- Ph RRRR/ 3- Ph SSSS ) [( R )- or ( S )-Ph-Pybox]4 EuIII 4 (BHP)6 , are presented with their structural and chiroptical characterization (BPP and BHP = bis-β-diketonates; Pybox = chiral bis(oxazolinyl) pyridine ligand). X-ray crystallographic analysis revealed that different extents of interligand-contacting interactions between bis-β-diketonates and Pybox ligands produce different bis-β-diketonates arrangements around four EuIII ions and, thus, their specific symmetry in the final tetranuclear complexes...