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https://www.readbyqxmd.com/read/28491487/biomolecular-engineering-for-nanobio-bionanotechnology
#1
REVIEW
Teruyuki Nagamune
Biomolecular engineering can be used to purposefully manipulate biomolecules, such as peptides, proteins, nucleic acids and lipids, within the framework of the relations among their structures, functions and properties, as well as their applicability to such areas as developing novel biomaterials, biosensing, bioimaging, and clinical diagnostics and therapeutics. Nanotechnology can also be used to design and tune the sizes, shapes, properties and functionality of nanomaterials. As such, there are considerable overlaps between nanotechnology and biomolecular engineering, in that both are concerned with the structure and behavior of materials on the nanometer scale or smaller...
2017: Nano Convergence
https://www.readbyqxmd.com/read/28414202/recent-advances-in-peptide-nucleic-acid-for-cancer-bionanotechnology
#2
REVIEW
Jun-Chen Wu, Qing-Chun Meng, Hong-Mei Ren, Hong-Tao Wang, Jie Wu, Qi Wang
Peptide nucleic acid (PNA) is an oligomer, in which the phosphate backbone has been replaced by a pseudopeptide backbone that is meant to mimic DNA. Peptide nucleic acids are of the utmost importance in the biomedical field because of their ability to hybridize with neutral nucleic acids and their special chemical and biological properties. In recent years, PNAs have emerged in nanobiotechnology for cancer diagnosis and therapy due to their high affinity and sequence selectivity toward corresponding DNA and RNA...
April 17, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28398221/amyloid-fibrils-from-hemoglobin
#3
Nadishka Jayawardena, Manmeet Kaur, Smitha Nair, Jenny Malmstrom, David Goldstone, Leonardo Negron, Juliet A Gerrard, Laura J Domigan
Amyloid fibrils are a class of insoluble protein nanofibers that are formed via the self-assembly of a wide range of peptides and proteins. They are increasingly exploited for a broad range of applications in bionanotechnology, such as biosensing and drug delivery, as nanowires, hydrogels, and thin films. Amyloid fibrils have been prepared from many proteins, but there has been no definitive characterization of amyloid fibrils from hemoglobin to date. Here, nanofiber formation was carried out under denaturing conditions using solutions of apo-hemoglobin extracted from bovine waste blood...
April 11, 2017: Biomolecules
https://www.readbyqxmd.com/read/28349997/applying-taguchi-design-and-large-scale-strategy-for-mycosynthesis-of-nano-silver-from-endophytic-trichoderma-harzianum-sya-f4-and-its-application-against-phytopathogens
#4
Shahira H El-Moslamy, Marwa F Elkady, Ahmed H Rezk, Yasser R Abdel-Fattah
Development of reliable and low-cost requirement for large-scale eco-friendly biogenic synthesis of metallic nanoparticles is an important step for industrial applications of bionanotechnology. In the present study, the mycosynthesis of spherical nano-Ag (12.7 ± 0.8 nm) from extracellular filtrate of local endophytic T. harzianum SYA.F4 strain which have interested mixed bioactive metabolites (alkaloids, flavonoids, tannins, phenols, nitrate reductase (320 nmol/hr/ml), carbohydrate (25 μg/μl) and total protein concentration (2...
March 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28337748/interaction-of-copper-nanoparticles-and-an-endophytic-growth-promoter-piriformospora-indica-with-cajanus-cajan
#5
Jyoti Rajak, Manisha Bawaskar, Dnyaneshwar Rathod, Gauravi Agarkar, Dipali Nagaonkar, Aniket Gade, Mahendra Rai
BACKGROUND: In recent years, agro-bionanotechnology has paved the way towards revolutionizing current practices in the food and agricultural fields. In the present study, the effect of copper nanoparticles (CuNPs) alone and in combination with the growth-promoting fungus Piriformospora indica on 2-week-old seedlings of Cajanus cajan was evaluated. Gelatin-coated stable CuNPs were synthesized by the chemical reduction method using hydrazine hydrate as a reducing agent. RESULTS: CuNPs were characterized by using UV-visible spectroscopy, zeta potential measurement and transmission electron microscopy...
March 23, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28317998/supramolecular-electrode-assemblies-for-bioelectrochemistry
#6
Theodoros Laftsoglou, Lars J C Jeuken
For more than three decades, the field of bioelectrochemistry has provided novel insights into the catalytic mechanisms of enzymes, the principles that govern biological electron transfer, and has elucidated the basic principles for bioelectrocatalytic systems. Progress in biochemistry, bionanotechnology, and our ever increasing ability to control the chemistry and structure of electrode surfaces has enabled the study of ever more complex systems with bioelectrochemistry. This feature article highlights developments over the last decade, where supramolecular approaches have been employed to develop electrode assemblies that increase enzyme loading on the electrode or create more biocompatible environments for membrane enzymes...
March 30, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28272871/nanoarchitectonics-with-porphyrin-functionalized-dna
#7
Eugen Stulz
DNA is well-known as bearer of the genetic code. Since its structure elucidation nearly seven decades ago by Watson, Crick, Wilkins, and Franklin, much has been learned about its detailed structure, function, and genetic coding. The development of automated solid-phase synthesis, and with it the availability of synthetic DNA with any desired sequence in lengths of up to hundreds of bases in the best case, has contributed much to the advancement of the field of DNA research. In addition, classic organic synthesis has allowed introduction of a very large number of modifications in the DNA in a sequence specific manner, which have initially been targeted at altering the biological function of DNA...
April 18, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28252973/dna-with-ionic-atomic-and-clustered-silver-an-xps-study
#8
Ivan L Volkov, Anastasiya Smirnova, Anna A Makarova, Zakhar V Reveguk, Ruslan R Ramazanov, Dmitry Yu Usachov, Vera K Adamchuk, Alexei I Kononov
The rapidly developing field of bionanotechnology requires detailed knowledge of the mechanisms of interaction between inorganic matter and biomolecules. Under conditions different from those in an aqueous solution, however, the chemistry of these systems is elusive and may differ dramatically from their interactions in vitro and in vivo. Here, we report for the first time a photoemission study of a metal silver-DNA interface, formed in vacuo, in comparison with DNA-Ag(+) and fluorescent DNA-Ag complexes formed in solution...
March 23, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28221767/construction-of-a-chassis-for-a-tripartite-protein-based-molecular-motor
#9
Lara S R Small, Marc Bruning, Andrew R Thomson, Aimee L Boyle, Roberta B Davies, Paul M G Curmi, Nancy R Forde, Heiner Linke, Derek N Woolfson, Elizabeth H C Bromley
Improving our understanding of biological motors, both to fully comprehend their activities in vital processes, and to exploit their impressive abilities for use in bionanotechnology, is highly desirable. One means of understanding these systems is through the production of synthetic molecular motors. We demonstrate the use of orthogonal coiled-coil dimers (including both parallel and antiparallel coiled coils) as a hub for linking other components of a previously described synthetic molecular motor, the Tumbleweed...
March 14, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28209060/use-of-spectroscopic-techniques-for-evaluating-the-coupling-of-porphyrins-on-biocompatible-nanoparticles-a-potential-system-for-photodynamics-theranostics-and-nanodrug-delivery-applications
#10
Lais N Magno, Fábio C Bezerra, Luiz Eduardo S Freire, Rubens A Guerra, Andris F Bakuzis, Pablo J Gonçalves
Modern medicine has been searching for new and more efficient strategies for diagnostics and therapeutics applications. Considering this, porphyrin molecules have received great interest for applications in photodiagnostics and phototherapies, even as magnetic nanoparticles for drug-delivery systems and magnetic-hyperthermia therapy. Aiming to obtain a multifunctional system, which combines diagnostics with therapeutic functions on the same platform, the present study employed UV/vis absorption and fluorescence spectroscopies to evaluate the interaction between meso-tetrakis(p-sulfonatofenyl)porphyrin (TPPS) and maghemite nanoparticles (γ-Fe2O3)...
March 9, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28024372/internal-hydration-properties-of-single-bacterial-endospores-probed-by-electrostatic-force-microscopy
#11
Marc Van Der Hofstadt, Rene Fabregas, Ruben Millan-Solsona, Antonio Juarez, Laura Fumagalli, Gabriel Gomila
We show that the internal hydration properties of single Bacillus cereus endospores in air under different relative humidity (RH) conditions can be determined through the measurement of its electric permittivity by means of quantitative electrostatic force microscopy (EFM). We show that an increase in the RH from 0% to 80% induces a large increase in the equivalent homogeneous relative electric permittivity of the bacterial endospores, from ∼4 up to ∼17, accompanied only by a small increase in the endospore height, of just a few nanometers...
December 27, 2016: ACS Nano
https://www.readbyqxmd.com/read/27990274/bacteriophage-based-tools-recent-advances-and-novel-applications
#12
REVIEW
Lisa O'Sullivan, Colin Buttimer, Olivia McAuliffe, Declan Bolton, Aidan Coffey
Bacteriophages (phages) are viruses that infect bacterial hosts, and since their discovery over a century ago they have been primarily exploited to control bacterial populations and to serve as tools in molecular biology. In this commentary, we highlight recent diverse advances in the field of phage research, going beyond bacterial control using whole phage, to areas including biocontrol using phage-derived enzybiotics, diagnostics, drug discovery, novel drug delivery systems and bionanotechnology.
2016: F1000Research
https://www.readbyqxmd.com/read/27922833/x-ray-photoelectron-spectroscopy-and-transmission-electron-microscopy-analysis-of-silver-coated-gold-nanorods-designed-for-bionanotechnology-applications
#13
Fumiya Watanabe, Zeid A Nima, Takumi Honda, Masatoshi Mitsuhara, Minoru Nishida, Alexandru S Biris
Multicomponent nano-agents were designed and built via a core-shell approach to enhance their surface enhanced Raman scattering (SERS) signals. These nano-agents had 36 nm × 12 nm gold nanorod cores coated by 4 nm thick silver shell films and a subsequent thin bifunctional thiolated polyethylene glycol (HS-PEG-COOH) layer. Ambient time-lapsed SERS signal measurements of these functionalized nanorods taken over a two-week period indicated no signal degradation, suggesting that large portions of the silver shells remained in pure metallic form...
January 13, 2017: Nanotechnology
https://www.readbyqxmd.com/read/27914059/evolution-inspired-computational-design-of-symmetric-proteins
#14
Arnout R D Voet, David Simoncini, Jeremy R H Tame, Kam Y J Zhang
Monomeric proteins with a number of identical repeats creating symmetrical structures are potentially very valuable building blocks with a variety of bionanotechnological applications. As such proteins do not occur naturally, the emerging field of computational protein design serves as an excellent tool to create them from nonsymmetrical templates. Existing pseudo-symmetrical proteins are believed to have evolved from oligomeric precursors by duplication and fusion of identical repeats. Here we describe a computational workflow to reverse-engineer this evolutionary process in order to create stable proteins consisting of identical sequence repeats...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27905946/chemically-and-thermally-stable-silica-nanowires-with-a-%C3%AE-sheet-peptide-core-for-bionanotechnology
#15
Zahraa S Al-Garawi, George E Kostakis, Louise C Serpell
BACKGROUND: A series of amyloidogenic peptides based on the sequence KFFEAAAKKFFE template the silica precursor, tetraethyl orthosilicate to form silica-nanowires containing a cross-β peptide core. RESULTS: Investigation of the stability of these fibres reveals that the silica layers protect the silica-nanowires allowing them to maintain their shape and physical and chemical properties after incubation with organic solvents such as 2-propanol, ethanol, and acetonitrile, as well as in a strong acidic solution at pH 1...
December 1, 2016: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/27866626/seaweeds-a-resource-for-marine-bionanotechnology
#16
REVIEW
Sri Ramkumar Vijayan, Prakash Santhiyagu, Ramasubburayan Ramasamy, Pugazhendhi Arivalagan, Gopalakrishnan Kumar, Kannapiran Ethiraj, Babu Rajendran Ramaswamy
Marine bionanotechnology is one of the most promising areas of research in modern science and technology. Although there are multitude methods for the synthesis of nanoparticles (NPs), there is an increasing attention in developing high-yield, low-cost, non-toxic and eco-friendly procedures. The vital advantages of greener synthesis are cost-effective, reduced usage of toxic chemicals and abundant availability of resources. During the last ten years, there have been many biological entities used to elevate novel, greener and affordable methods for the metal NPs synthesis...
December 2016: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27783468/design-of-nanoscale-enzyme-complexes-based-on-various-scaffolding-materials-for-biomass-conversion-and-immobilization
#17
REVIEW
Jeong Eun Hyeon, Sang Kyu Shin, Sung Ok Han
The utilization of scaffolds for enzyme immobilization involves advanced bionanotechnology applications in biorefinery fields, which can be achieved by optimizing the function of various enzymes. This review presents various current scaffolding techniques based on proteins, microbes and nanomaterials for enzyme immobilization, as well as the impact of these techniques on the biorefinery of lignocellulosic materials. Among them, architectural scaffolds have applied to useful strategies for protein engineering to improve the performance of immobilized enzymes in several industrial and research fields...
November 2016: Biotechnology Journal
https://www.readbyqxmd.com/read/27700108/toward-single-electron-nanoelectronics-using-self-assembled-dna-structure
#18
Kosti Tapio, Jenni Leppiniemi, Boxuan Shen, Vesa P Hytönen, Wolfgang Fritzsche, J Jussi Toppari
DNA based structures offer an adaptable and robust way to develop customized nanostructures for various purposes in bionanotechnology. One main aim in this field is to develop a DNA nanobreadboard for a controllable attachment of nanoparticles or biomolecules to form specific nanoelectronic devices. Here we conjugate three gold nanoparticles on a defined size TX-tile assembly into a linear pattern to form nanometer scale isolated islands that could be utilized in a room temperature single electron transistor...
November 9, 2016: Nano Letters
https://www.readbyqxmd.com/read/27569200/antimicrobial-graphene-family-materials-progress-advances-hopes-and-fears
#19
REVIEW
Anna Lukowiak, Anna Kedziora, Wieslaw Strek
Graphene-based materials have become very popular bionanotechnological instruments in the last few years. Since 2010, the graphene family materials have been recognized as worthy of attention due to its antimicrobial properties. Functionalization of graphene (or rather graphene oxide) surface creates the possibilities to obtain efficient antimicrobial agents. In this review, progress and advances in this field in the last few years are described and discussed. Special attention is devoted to materials based on graphene oxide in which specifically selected components significantly modify biological activity of this carbon structure...
October 2016: Advances in Colloid and Interface Science
https://www.readbyqxmd.com/read/27540604/durable-proteo-hybrid-vesicles-for-the-extended-functional-lifetime-of-membrane-proteins-in-bionanotechnology
#20
Sanobar Khan, Mengqiu Li, Stephen P Muench, Lars J C Jeuken, Paul A Beales
The full capabilities of membrane proteins in bionanotechnology can only be realised through improvements in their reconstitution environments that combine biocompatibility to support function and durability for long term stability. We demonstrate that hybrid vesicles composed of natural phospholipids and synthetic diblock copolymers have the potential to achieve these criteria.
September 21, 2016: Chemical Communications: Chem Comm
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