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https://www.readbyqxmd.com/read/29101307/combining-high-resolution-cryo-electron-microscopy-and-mutagenesis-to-develop-cowpea-mosaic-virus-for-bionanotechnology
#1
REVIEW
Yulia Meshcheriakova, Alex Durrant, Emma L Hesketh, Neil A Ranson, George P Lomonossoff
Particles of cowpea mosaic virus (CPMV) have enjoyed considerable success as nanoparticles. The development of a system for producing empty virus-like particles (eVLPs) of the virus, which are non-infectious and have the potential to be loaded with heterologous material, has increased the number of possible applications for CPMV-based particles. However, for this potential to be realised, it was essential to demonstrate that eVLPs were accurate surrogates for natural virus particles, and this information was provided by high-resolution cryo-EM studies of eVLPs...
November 3, 2017: Biochemical Society Transactions
https://www.readbyqxmd.com/read/29074309/fully-artificial-exosomes-towards-new-theranostic-biomaterials
#2
Pablo García-Manrique, Gemma Gutiérrez, Maria Carmen Blanco-López
Bionanotechnology routes have been recently developed to produce fully artificial exosomes: biomimetic particles designed to overcome certain limitations in extracellular vesicle (EV) biology and applications. These particles could soon become true therapeutic biomaterials. Here, we outline their current preparation techniques, their explored and future possibilities, and their present limits.
October 23, 2017: Trends in Biotechnology
https://www.readbyqxmd.com/read/29055106/streptavidin-coated-surfaces-suppress-bacterial-colonisation-by-inhibiting-non-specific-protein-adsorption
#3
Volker Ettelt, Katharina Ekat, Peer W Kämmerer, Bernd Kreikemeyer, Matthias Epple, Michael Veith
Streptavidin is a 58 kDa tetrameric protein with the highest known affinity to biotin with a wide range of applications in bionanotechnology and molecular biology. Dissolved streptavidin is stable at a broad range of temperature, pH, proteolytic enzymes and exhibits low non-specific binding. In this study, a streptavidin monolayer was assembled directly on a biotinylated TiO2 -surface to investigate its stability against proteolytic digestion and its suppression of initial bacterial adsorption of Escherichia coli, Bacillus subtilis, and Streptococcus intermedius...
October 21, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28993826/effect-of-size-and-curvature-on-the-enzyme-activity-of-bionanoconjugates
#4
Sirimuvva Tadepalli, Zheyu Wang, Joseph Slocik, Rajesh R Naik, Srikanth Singamaneni
Biotic-abiotic hybrids comprised of globular proteins and functional nanostructures with complementary and synergistic properties are central to a number of bionanotechnological applications. A comprehensive understanding of the effect of physicochemical properties of abiotic nanostructures on the biological activity of the bionanoconjugates is critical in the design of these bio-nano hybrids. In this study, using size and curvature-controlled gold nanoparticles as a model abiotic system, we investigated the effect of hydrodynamic diameter and surface curvature on the activity of a model enzyme, horseradish peroxidase (HRP), adsorbed on the surface of the nanostructures...
October 19, 2017: Nanoscale
https://www.readbyqxmd.com/read/28985740/nanoscale-click-reactive-scaffolds-from-peptide-self-assembly
#5
Alexander P M Guttenplan, Laurence J Young, Dijana Matak-Vinkovic, Clemens F Kaminski, Tuomas P J Knowles, Laura S Itzhaki
BACKGROUND: Due to their natural tendency to self-assemble, proteins and peptides are important components for organic nanotechnology. One particular class of peptides of recent interest is those that form amyloid fibrils, as this self-assembly results in extremely strong, stable quasi-one-dimensional structures which can be used to organise a wide range of cargo species including proteins and oligonucleotides. However, assembly of peptides already conjugated to proteins is limited to cargo species that do not interfere sterically with the assembly process or misfold under the harsh conditions often used for assembly...
October 6, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/28900011/robust-mechanobiological-behavior-emerges-in-heterogeneous-myosin-systems
#6
Paul F Egan, Jeffrey R Moore, Allen J Ehrlicher, David A Weitz, Christian Schunn, Jonathan Cagan, Philip LeDuc
Biological complexity presents challenges for understanding natural phenomenon and engineering new technologies, particularly in systems with molecular heterogeneity. Such complexity is present in myosin motor protein systems, and computational modeling is essential for determining how collective myosin interactions produce emergent system behavior. We develop a computational approach for altering myosin isoform parameters and their collective organization, and support predictions with in vitro experiments of motility assays with α-actinins as molecular force sensors...
September 26, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28878782/in-planta-synthesis-of-designer-length-tobacco-mosaic-virus-based-nano-rods-that-can-be-used-to-fabricate-nano-wires
#7
Keith Saunders, George P Lomonossoff
We have utilized plant-based transient expression to produce tobacco mosaic virus (TMV)-based nano-rods of predetermined lengths. This is achieved by expressing RNAs containing the TMV origin of assembly sequence (OAS) and the sequence of the TMV coat protein either on the same RNA molecule or on two separate constructs. We show that the length of the resulting nano-rods is dependent upon the length of the RNA that possesses the OAS element. By expressing a version of the TMV coat protein that incorporates a metal-binding peptide at its C-terminus in the presence of RNA containing the OAS we have been able to produce nano-rods of predetermined length that are coated with cobalt-platinum...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28846925/interaction-of-extremophilic-archaeal-viruses-with-human-and-mouse-complement-system-and-viral-biodistribution-in-mice
#8
Linping Wu, Kristine Buch Uldahl, Fangfang Chen, Halli Benasutti, Deborah Logvinski, Vivian Vu, Nirmal K Banda, Xu Peng, Dmitri Simberg, Seyed Moein Moghimi
Archaeal viruses offer exceptional biophysical properties for modification and exploration of their potential in bionanotechnology, bioengineering and nanotherapeutic developments. However, the interaction of archaeal viruses with elements of the innate immune system has not been explored, which is a necessary prerequisite if their potential for biomedical applications to be realized. Here we show complement activation through lectin (via direct binding of MBL/MASPs) and alternative pathways by two extremophilic archaeal viruses (Sulfolobus monocaudavirus 1 and Sulfolobus spindle-shaped virus 2) in human serum...
October 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28796191/smart-carriers-and-nanohealers-a-nanomedical-insight-on-natural-polymers
#9
REVIEW
Sreejith Raveendran, Ankit K Rochani, Toru Maekawa, D Sakthi Kumar
Biodegradable polymers are popularly being used in an increasing number of fields in the past few decades. The popularity and favorability of these materials are due to their remarkable properties, enabling a wide range of applications and market requirements to be met. Polymer biodegradable systems are a promising arena of research for targeted and site-specific controlled drug delivery, for developing artificial limbs, 3D porous scaffolds for cellular regeneration or tissue engineering and biosensing applications...
August 10, 2017: Materials
https://www.readbyqxmd.com/read/28794934/activity-of-chitosan-lysozyme-nanoparticles-on-the-growth-membrane-integrity-and-%C3%AE-1-3-glucanase-production-by-aspergillus-parasiticus
#10
Cynthia Nazareth Hernández-Téllez, Francisco Julián Rodríguez-Córdova, Ema Carina Rosas-Burgos, Mario Onofre Cortez-Rocha, Armando Burgos-Hernández, Jaime Lizardi-Mendoza, Wilfrido Torres-Arreola, Aarón Martínez-Higuera, Maribel Plascencia-Jatomea
Synthesis of nanocomposites from antimicrobial biopolymers such as chitosan (CS) and lysozyme (LZ) is an important and promising area in bionanotechnology. Chitosan-lysozyme (CS-LZ) nanoparticles (NPs) were prepared by the nanoprecipitation method, using commercial chitosan of 153 kDa. TEM and dynamic light scattering (DLS) analysis were carried out to evaluate the morphology, size, dispersion, and Z potential. Association efficiency of lysozyme was determined using Coomassie blue assay. The antifungal activity of NPs against Aspergillus parasiticus was evaluated through cell viability (XTT), germination and morphometry of spores, and reducing sugars production; the effects on membrane integrity and cell wall were also analyzed...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28773125/lateral-tension-induced-penetration-of-particles-into-a-liposome
#11
Kazuki Shigyou, Ken H Nagai, Tsutomu Hamada
It is important that we understand the mechanism of the penetration of particles into a living cell to achieve advances in bionanotechnology, such as for treatment, visualization within a cell, and genetic modification. Although there have been many studies on the application of functional particles to cells, the basic mechanism of penetration across a biological membrane is still poorly understood. Here we used a model membrane system to demonstrate that lateral membrane tension drives particle penetration across a lipid bilayer...
July 7, 2017: Materials
https://www.readbyqxmd.com/read/28749677/nature-inspired-construction-of-two-dimensionally-self-assembled-peptide-on-pristine-graphene
#12
Young Hyun No, Nam Hyeong Kim, Bramaramba Gnapareddy, Bumjoon Choi, Yong-Tae Kim, Sreekantha Reddy Dugasani, One-Sun Lee, Kook-Han Kim, Young-Seon Ko, Seungwoo Lee, Sang Woo Lee, Sung Ha Park, Kilho Eom, Yong Ho Kim
Peptide assemblies have received significant attention because of their important role in biology and applications in bionanotechnology. Despite recent efforts to elucidate the principles of peptide self-assembly for developing novel functional devices, peptide self-assembly on two-dimensional nanomaterials has remained challenging. Here, we report nature-inspired two-dimensional peptide self-assembly on pristine graphene via optimization of peptide-peptide and peptide-graphene interactions. Two-dimensional peptide self-assembly was designed based on statistical analyses of >10(4) protein structures existing in nature and atomistic simulation-based structure predictions...
July 31, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28722303/differential-electrochemical-conductance-imaging-at-the-nanoscale
#13
Montserrat López-Martínez, Juan Manuel Artés, Veronica Sarasso, Marco Carminati, Ismael Díez-Pérez, Fausto Sanz, Pau Gorostiza
Electron transfer in proteins is essential in crucial biological processes. Although the fundamental aspects of biological electron transfer are well characterized, currently there are no experimental tools to determine the atomic-scale electronic pathways in redox proteins, and thus to fully understand their outstanding efficiency and environmental adaptability. This knowledge is also required to design and optimize biomolecular electronic devices. In order to measure the local conductance of an electrode surface immersed in an electrolyte, this study builds upon the current-potential spectroscopic capacity of electrochemical scanning tunneling microscopy, by adding an alternating current modulation technique...
July 19, 2017: Small
https://www.readbyqxmd.com/read/28682599/thermophilic-ferritin-24mer-assembly-and-nanoparticle-encapsulation-modulated-by-interdimer-electrostatic-repulsion
#14
Katherine W Pulsipher, Jose A Villegas, Benjamin W Roose, Tacey L Hicks, Jennifer Yoon, Jeffery G Saven, Ivan J Dmochowski
Protein cage self-assembly enables encapsulation and sequestration of small molecules, macromolecules, and nanomaterials for many applications in bionanotechnology. Notably, wild-type thermophilic ferritin from Archaeoglobus fulgidus (AfFtn) exists as a stable dimer of four-helix bundle proteins at a low ionic strength, and the protein forms a hollow assembly of 24 protomers at a high ionic strength (∼800 mM NaCl). This assembly process can also be initiated by highly charged gold nanoparticles (AuNPs) in solution, leading to encapsulation...
July 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28605903/influence-of-surface-charge-of-the-nanostructures-on-the-biocatalytic-activity
#15
Sirimuvva Tadepalli, Zheyu Wang, Keng-Ku Liu, Qisheng Jiang, Joseph Slocik, Rajesh R Naik, Srikanth Singamaneni
The physicochemical properties of abiotic nanostructures determine the structure and function of biological counterparts in biotic-abiotic nanohybrids. A comprehensive understanding of the interfacial interactions and the predictive capability of their structure and function is paramount for virtually all fields of bionanotechnology. In this study, using plasmonic nanostructures as a model abiotic system, we investigate the effect of the surface charge of nanostructures on the biocatalytic reaction kinetics of a bound enzyme...
June 22, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28491487/biomolecular-engineering-for-nanobio-bionanotechnology
#16
REVIEW
Teruyuki Nagamune
Biomolecular engineering can be used to purposefully manipulate biomolecules, such as peptides, proteins, nucleic acids and lipids, within the framework of the relations among their structures, functions and properties, as well as their applicability to such areas as developing novel biomaterials, biosensing, bioimaging, and clinical diagnostics and therapeutics. Nanotechnology can also be used to design and tune the sizes, shapes, properties and functionality of nanomaterials. As such, there are considerable overlaps between nanotechnology and biomolecular engineering, in that both are concerned with the structure and behavior of materials on the nanometer scale or smaller...
2017: Nano Convergence
https://www.readbyqxmd.com/read/28414202/recent-advances-in-peptide-nucleic-acid-for-cancer-bionanotechnology
#17
REVIEW
Jun-Chen Wu, Qing-Chun Meng, Hong-Mei Ren, Hong-Tao Wang, Jie Wu, Qi Wang
Peptide nucleic acid (PNA) is an oligomer, in which the phosphate backbone has been replaced by a pseudopeptide backbone that is meant to mimic DNA. Peptide nucleic acids are of the utmost importance in the biomedical field because of their ability to hybridize with neutral nucleic acids and their special chemical and biological properties. In recent years, PNAs have emerged in nanobiotechnology for cancer diagnosis and therapy due to their high affinity and sequence selectivity toward corresponding DNA and RNA...
June 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28398221/amyloid-fibrils-from-hemoglobin
#18
Nadishka Jayawardena, Manmeet Kaur, Smitha Nair, Jenny Malmstrom, David Goldstone, Leonardo Negron, Juliet A Gerrard, Laura J Domigan
Amyloid fibrils are a class of insoluble protein nanofibers that are formed via the self-assembly of a wide range of peptides and proteins. They are increasingly exploited for a broad range of applications in bionanotechnology, such as biosensing and drug delivery, as nanowires, hydrogels, and thin films. Amyloid fibrils have been prepared from many proteins, but there has been no definitive characterization of amyloid fibrils from hemoglobin to date. Here, nanofiber formation was carried out under denaturing conditions using solutions of apo-hemoglobin extracted from bovine waste blood...
April 11, 2017: Biomolecules
https://www.readbyqxmd.com/read/28349997/applying-taguchi-design-and-large-scale-strategy-for-mycosynthesis-of-nano-silver-from-endophytic-trichoderma-harzianum-sya-f4-and-its-application-against-phytopathogens
#19
Shahira H El-Moslamy, Marwa F Elkady, Ahmed H Rezk, Yasser R Abdel-Fattah
Development of reliable and low-cost requirement for large-scale eco-friendly biogenic synthesis of metallic nanoparticles is an important step for industrial applications of bionanotechnology. In the present study, the mycosynthesis of spherical nano-Ag (12.7 ± 0.8 nm) from extracellular filtrate of local endophytic T. harzianum SYA.F4 strain which have interested mixed bioactive metabolites (alkaloids, flavonoids, tannins, phenols, nitrate reductase (320 nmol/hr/ml), carbohydrate (25 μg/μl) and total protein concentration (2...
March 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28337748/interaction-of-copper-nanoparticles-and-an-endophytic-growth-promoter-piriformospora-indica-with-cajanus-cajan
#20
Jyoti Rajak, Manisha Bawaskar, Dnyaneshwar Rathod, Gauravi Agarkar, Dipali Nagaonkar, Aniket Gade, Mahendra Rai
BACKGROUND: In recent years, agro-bionanotechnology has paved the way towards revolutionizing current practices in the food and agricultural fields. In the present study, the effect of copper nanoparticles (CuNPs) alone and in combination with the growth-promoting fungus Piriformospora indica on 2-week-old seedlings of Cajanus cajan was evaluated. Gelatin-coated stable CuNPs were synthesized by the chemical reduction method using hydrazine hydrate as a reducing agent. RESULTS: CuNPs were characterized by using UV-visible spectroscopy, zeta potential measurement and transmission electron microscopy...
March 23, 2017: Journal of the Science of Food and Agriculture
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