Read by QxMD icon Read


Xuming Sun, Lin Huang, Ru Zhang, Wei Xu, Jingyi Huang, Deepanjali D Gurav, Vadanasundari Vedarethinam, Ruoping Chen, Jiatao Lou, Qian Wang, Jingjing Wan, Kun Qian
Current metabolic analysis is far from ideal to engage clinics and needs rationally designed materials and device. Here we developed a novel plasmonic chip for clinical metabolic fingerprinting. We first constructed a series of chips with gold nanoshells on the surface through controlled particle synthesis, dip-coating, and gold sputtering for mass production. We integrated the optimized chip with microarrays for laboratory automation and micro-/nanoscaled experiments, which afforded direct high-performance metabolic fingerprinting by laser desorption/ionization mass spectrometry using 500 nL of various biofluids and exosomes...
February 28, 2018: ACS Central Science
Bengang Xing, Xiangzhao Ai, Ming Hu, Zhimin Wang, Wenmin Zhang, Juan Li, Jun Lin, Huang-Hao Yang
In terms of the extremely small size and large specific surface area, nanomaterials often exhibit unusual physical and chemical properties, which have recently attracted considerable attention in bionanotechnology and nanomedicine. Currently, the extensive usage of nanotechnology in medicine holds the great potential for precise diagnosis and effective therapeutics of various human diseases in clinical practice in past decades. However, a detailed understanding regarding how nanomedicines interact with the intricate environment in complex living systems remains a pressing and challenging goal...
March 6, 2018: Bioconjugate Chemistry
Xiaogang Qu, Hanjun Sun, Ya Zhou, Jinsong Ren
Nanozymes have advantages over natural enzymes, such as facile production on large scale, long storage time, low costs, and high stability in harsh environments. Nanocarbon materials (CNMs), including fullerene, carbon nanotube, graphene, carbon quantum dots, graphene quantum dots, etc., have become a star family in materials science. As a new class of nanozymes, the catalytic activity of CNMs and their hybrids has been extensively reported. In this minireview, recent progress of CNMs based artificial enzymes have been summarized...
March 5, 2018: Angewandte Chemie
Sabine Szunerits, Rabah Boukherroub
Advances in materials science and bionanotechnology have allowed the refinements of current drug delivery systems, expected to facilitate the development of personalized medicine. While dermatological topical pharmaceutical formulations such as foams, creams, lotions, gels, etc., have been proposed for decades, these systems target mainly skin-based diseases. To treat systemic medical conditions as well as localized problems such as joint or muscle concerns, transdermal delivery systems (TDDSs), which use the skin as the main route of drug delivery, are very appealing...
2018: Frontiers in Bioengineering and Biotechnology
Hiroki Noguchi, Bram Mylemans, Elke De Zitter, Luc Van Meervelt, Jeremy R H Tame, Arnout Voet
β-propeller proteins are highly symmetrical, being composed of a repeated motif with four anti-parallel β-sheets arranged around a central axis. Recently we designed the first completely symmetrical β-propeller protein, Pizza6, consisting of six identical tandem repeats. Pizza6 is expected to prove a useful building block for bionanotechnology, and also a tool to investigate the folding and evolution of β-propeller proteins. Folding studies are made difficult by the high stability and the lack of buried Trp residues to act as monitor fluorophores, so we have designed and characterized several Trp-containing Pizza6 derivatives...
February 23, 2018: Biochemical and Biophysical Research Communications
Anasua Mukhopadhayay, Tarasankar Das, Anindya Datta, Kamendra P Sharma
Solvents particularly those having low-volatility are of great interest for the biocatalytic synthesis of utility chemicals and fuels. Here we show novel and universal solvent-like properties of a neat and water-less polymer surfactant-BSA conjugated material (WL-PSpBSA). This highly viscous, non-volatile material, behaves as a liquid above its solid-liquid transition temperature (≈ 25-27 °C), and can be used as a solvent for variety of completely dry solutes of different sizes, and surface chemistries. We show using a combination of optical microscopy, steady state and time-resolved fluoresence spectroscopy, that dry and powdered dyes (hydrophobic Coumarin 153; C153), enzymes (α-chymotrypsin; α-Chy), or even 2 μm microparticles (diffusion coefficient ca...
February 16, 2018: Biomacromolecules
Stéphanie Devineau, Laurent Kiger, Frédéric Galacteros, Véronique Baudin-Creuza, Michael Marden, Jean Philippe Renault, Serge Pin
Recently, nanoparticles have attracted much attention as new scaffolds for hemoglobin-based oxygen carriers (HBOCs). Indeed, the development of bionanotechnology paves the way for the rational design of blood substitutes, providing that the interaction between the nanoparticles and hemoglobin at a molecular scale and its effect on the oxygenation properties of hemoglobin are finely controlled. Here, we show that human hemoglobin has a high affinity for silica nanoparticles, leading to the adsorption of hemoglobin tetramers on the surface...
January 23, 2018: Blood Advances
Anna Epanchintseva, Pavel Vorobjev, Dmitrii Pyshnyi, Inna Pyshnaya
The adsorption of oligonucleotides on citrate-coated gold nanoparticles (AuNPs) is studied under conditions "right after the synthesis", i.e., in a weak citrate solution at a pH value close to neutral (5.8 ± 0.2). We found that short-term elevation of reaction temperature under these conditions provides fast and strong adsorption of oligonucleotides on the surface of AuNPs. The affinity of oligonucleotides to AuNPs depends on the length of the oligonucleotide and its nucleotide composition. The shortest oligonucleotide in this study, T6 , is the most affine, having the equilibrium binding constant KD = 0...
January 9, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Kai Huang, Haichun Liu, Marco Kraft, Swati Shikha, Xiang Zheng, Hans Ågren, Christian Würth, Ute Resch-Genger, Yong Zhang
Lanthanide-doped upconversion nanoparticles (UCNPs) are of great interest for biomedical applications. Currently, the applicability of UCNP bionanotechnology is hampered by the generally low luminescence intensity of UCNPs and inefficient energy transfer from UCNPs to surface-bound chromophores used e.g. for photodynamic therapy or analyte sensing. In this work, we address the low-efficiency issue by developing versatile core-shell nanostructures, where high-concentration sensitizers and activators are confined in the core and shell region of representative hexagonal NaYF4 :Yb,Er UCNPs...
December 21, 2017: Nanoscale
Yibo Zhou, Zhicheng Huang, Ronghua Yang, Juewen Liu
Searching for DNA sequences that can strongly and selectively bind to inorganic surfaces is a long-standing topic in bionanotechnology, analytical chemistry and biointerface research. This can be achieved either by aptamer selection starting with a very large library of ≈1014 random DNA sequences, or by careful screening of a much smaller library (usually from a few to a few hundred) with rationally designed sequences. Unlike typical molecular targets, inorganic surfaces often have quite strong DNA adsorption affinities due to polyvalent binding and even chemical interactions...
February 21, 2018: Chemistry: a European Journal
Eric Beamish, Vincent Tabard-Cossa, Michel Godin
The identification of molecular tags along nucleic acid sequences has many potential applications in bionanotechnology, disease biomarker detection, and DNA sequencing. An attractive approach to this end is the use of solid-state nanopores, which can electrically detect molecular substructure and can be integrated into portable lab-on-a-chip sensors. We present here a DNA origami-based approach of molecular assembly in which solid-state nanopores are capable of differentiating 165 bp scaffolds containing zero, one, and two dsDNA protrusions...
December 22, 2017: ACS Sensors
Yulia Meshcheriakova, Alex Durrant, Emma L Hesketh, Neil A Ranson, George P Lomonossoff
Particles of cowpea mosaic virus (CPMV) have enjoyed considerable success as nanoparticles. The development of a system for producing empty virus-like particles (eVLPs) of the virus, which are non-infectious and have the potential to be loaded with heterologous material, has increased the number of possible applications for CPMV-based particles. However, for this potential to be realised, it was essential to demonstrate that eVLPs were accurate surrogates for natural virus particles, and this information was provided by high-resolution cryo-EM studies of eVLPs...
November 3, 2017: Biochemical Society Transactions
Pablo García-Manrique, Gemma Gutiérrez, Maria Carmen Blanco-López
Bionanotechnology routes have been recently developed to produce fully artificial exosomes: biomimetic particles designed to overcome certain limitations in extracellular vesicle (EV) biology and applications. These particles could soon become true therapeutic biomaterials. Here, we outline their current preparation techniques, their explored and future possibilities, and their present limits.
October 23, 2017: Trends in Biotechnology
Volker Ettelt, Katharina Ekat, Peer W Kämmerer, Bernd Kreikemeyer, Matthias Epple, Michael Veith
Streptavidin is a 58 kDa tetrameric protein with the highest known affinity to biotin with a wide range of applications in bionanotechnology and molecular biology. Dissolved streptavidin is stable at a broad range of temperature, pH, proteolytic enzymes and exhibits low non-specific binding. In this study, a streptavidin monolayer was assembled directly on a biotinylated TiO2 -surface to investigate its stability against proteolytic digestion and its suppression of initial bacterial adsorption of Escherichia coli, Bacillus subtilis, and Streptococcus intermedius...
October 21, 2017: Journal of Biomedical Materials Research. Part A
Sirimuvva Tadepalli, Zheyu Wang, Joseph Slocik, Rajesh R Naik, Srikanth Singamaneni
Biotic-abiotic hybrids comprised of globular proteins and functional nanostructures with complementary and synergistic properties are central to a number of bionanotechnological applications. A comprehensive understanding of the effect of physicochemical properties of abiotic nanostructures on the biological activity of the bionanoconjugates is critical in the design of these bio-nano hybrids. In this study, using size and curvature-controlled gold nanoparticles as a model abiotic system, we investigated the effect of hydrodynamic diameter and surface curvature on the activity of a model enzyme, horseradish peroxidase (HRP), adsorbed on the surface of the nanostructures...
October 19, 2017: Nanoscale
Alexander P M Guttenplan, Laurence J Young, Dijana Matak-Vinkovic, Clemens F Kaminski, Tuomas P J Knowles, Laura S Itzhaki
BACKGROUND: Due to their natural tendency to self-assemble, proteins and peptides are important components for organic nanotechnology. One particular class of peptides of recent interest is those that form amyloid fibrils, as this self-assembly results in extremely strong, stable quasi-one-dimensional structures which can be used to organise a wide range of cargo species including proteins and oligonucleotides. However, assembly of peptides already conjugated to proteins is limited to cargo species that do not interfere sterically with the assembly process or misfold under the harsh conditions often used for assembly...
October 6, 2017: Journal of Nanobiotechnology
Paul F Egan, Jeffrey R Moore, Allen J Ehrlicher, David A Weitz, Christian Schunn, Jonathan Cagan, Philip LeDuc
Biological complexity presents challenges for understanding natural phenomenon and engineering new technologies, particularly in systems with molecular heterogeneity. Such complexity is present in myosin motor protein systems, and computational modeling is essential for determining how collective myosin interactions produce emergent system behavior. We develop a computational approach for altering myosin isoform parameters and their collective organization, and support predictions with in vitro experiments of motility assays with α-actinins as molecular force sensors...
September 26, 2017: Proceedings of the National Academy of Sciences of the United States of America
Keith Saunders, George P Lomonossoff
We have utilized plant-based transient expression to produce tobacco mosaic virus (TMV)-based nano-rods of predetermined lengths. This is achieved by expressing RNAs containing the TMV origin of assembly sequence (OAS) and the sequence of the TMV coat protein either on the same RNA molecule or on two separate constructs. We show that the length of the resulting nano-rods is dependent upon the length of the RNA that possesses the OAS element. By expressing a version of the TMV coat protein that incorporates a metal-binding peptide at its C-terminus in the presence of RNA containing the OAS we have been able to produce nano-rods of predetermined length that are coated with cobalt-platinum...
2017: Frontiers in Plant Science
Linping Wu, Kristine Buch Uldahl, Fangfang Chen, Halli Benasutti, Deborah Logvinski, Vivian Vu, Nirmal K Banda, Xu Peng, Dmitri Simberg, Seyed Moein Moghimi
Archaeal viruses offer exceptional biophysical properties for modification and exploration of their potential in bionanotechnology, bioengineering and nanotherapeutic developments. However, the interaction of archaeal viruses with elements of the innate immune system has not been explored, which is a necessary prerequisite if their potential for biomedical applications to be realized. Here we show complement activation through lectin (via direct binding of MBL/MASPs) and alternative pathways by two extremophilic archaeal viruses (Sulfolobus monocaudavirus 1 and Sulfolobus spindle-shaped virus 2) in human serum...
October 2017: Molecular Immunology
Sreejith Raveendran, Ankit K Rochani, Toru Maekawa, D Sakthi Kumar
Biodegradable polymers are popularly being used in an increasing number of fields in the past few decades. The popularity and favorability of these materials are due to their remarkable properties, enabling a wide range of applications and market requirements to be met. Polymer biodegradable systems are a promising arena of research for targeted and site-specific controlled drug delivery, for developing artificial limbs, 3D porous scaffolds for cellular regeneration or tissue engineering and biosensing applications...
August 10, 2017: Materials
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"