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https://www.readbyqxmd.com/read/28817704/analysis-of-multidrug-resistant-group-b-streptococci-with-reduced-penicillin-susceptibility-forming-small-less-hemolytic-colonies
#1
Hirotsugu Banno, Kouji Kimura, Yosuke Tanaka, Tsuyoshi Sekizuka, Makoto Kuroda, Wanchun Jin, Jun-Ichi Wachino, Keiko Yamada, Keigo Shibayama, Yoshichika Arakawa
Group B streptococci (GBS; Streptococcus agalactiae) are the leading cause of neonatal invasive diseases and are also important pathogens for elderly adults. Until now, nearly all GBS with reduced penicillin susceptibility (PRGBS) have shown β-hemolytic activity and grow on sheep blood agar. However, we have previously reported three PRGBS clinical isolates harboring a CylK deletion that form small less hemolytic colonies. In this study, we examined the causes of small, less hemolytic colony formation in these clinical isolates...
2017: PloS One
https://www.readbyqxmd.com/read/28801661/statistical-characterization-of-therapeutic-protein-modifications
#2
Tsung-Heng Tsai, Zhiqi Hao, Qiuting Hong, Benjamin Moore, Cinzia Stella, Jeffrey H Zhang, Yan Chen, Michael Kim, Theo Koulis, Gregory A Ryslik, Erik Verschueren, Fred Jacobson, William E Haskins, Olga Vitek
Peptide mapping with liquid chromatography-tandem mass spectrometry (LC-MS/MS) is an important analytical method for characterization of post-translational and chemical modifications in therapeutic proteins. Despite its importance, there is currently no consensus on the statistical analysis of the resulting data. In this manuscript, we distinguish three statistical goals for therapeutic protein characterization: (1) estimation of site occupancy of modifications in one condition, (2) detection of differential site occupancy between conditions, and (3) estimation of combined site occupancy across multiple modification sites...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28747897/identification-of-emerging-human-mastitis-pathogens-by-maldi-tof-and-assessment-of-their-antibiotic-resistance-patterns
#3
María Marín, Rebeca Arroyo, Irene Espinosa-Martos, Leónides Fernández, Juan M Rodríguez
Lactational mastitis constitutes one of the main causes of undesired weaning, depriving the mother-infant pair from the benefits of breastfeeding; therefore, this condition should be considered a relevant public health issue. The role of specific microorganisms remains unclear since human milk cultures and antibiotic susceptibility testing (AST) are not routinely performed, despite the fact that this would be key to ensure an early and effective diagnosis and treatment. The objective of this study was to describe the culturable microbial diversity in 647 milk samples from breastfeeding women with clinical symptoms of mastitis by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF) VITEK MS technology and to analyze the antimicrobial susceptibility profiles of a collection of isolates from these samples by the VITEK 2 AST system...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28730548/orthopedic-infections-caused-by-obligatory-anaerobic-gram-negative-rods-report-of-two-cases
#4
Marta Kierzkowska, Piotr Pedzisz, Ireneusz Babiak, Jakub Janowicz, Mateusz Kulig, Anna Majewska, Anna Sawicka-Grzelak, Grazyna Mlynarczyk
Anaerobic bone and joint infections are uncommon, although the number of anaerobic infections is presumably underestimated because of difficulties with isolation and identification of obligate anaerobes. This study describes two cases of complicated Bacteroides fragilis peri-implant infection of the lumbar spine, infection of the hip and osteomyelitis. Bacteria were identified with the use of a mass spectrometer, VITEK MS system. Drug susceptibility was performed with the use of E-test. The EUCAST breakpoints were used for interpretation with B...
July 20, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28668254/corrigendum-to-maldi-tof-ms-is-more-accurate-than-vitek-ii-anc-card-and-api-rapid-id-32-a-system-for-the-identification-of-clostridium-species-anaerobe-40-2016-73-75
#5
Young Jin Kim, Si Hyun Kim, Hyun-Jung Park, Hae-Geun Park, Dongchul Park, Sae Am Song, Hee Joo Lee, Dongeun Yong, Jun Yong Choi, Joong-Ki Kook, Hye Ran Kim, Jeong Hwan Shin
No abstract text is available yet for this article.
June 28, 2017: Anaerobe
https://www.readbyqxmd.com/read/28658756/unusual-severe-extra-intestinal-manifestations-of-a-common-enteric-pathogen-aeromonas-spp
#6
Barnini Banerjee, Mridula Madiyal, Lingadakai Ramchandra, Chiranjay Mukhopadhyay, Rahul Garg, Kiran Chawla
INTRODUCTION: Aeromonas spp. are halophilic, Gram-negative bacilli. They are widely distributed in the soil and aquatic environment and have been associated with various extra-intestinal infections, such as skin and soft-tissue infections, meningitis, bacteraemia etc. The most common species associated with extra-intestinal infection is Aeromonas hydrophila. AIM: To get the overview of clinical presentations, underlying predisposing factors associated with the extra-intestinal infections caused by Aeromonas species and their antimicrobial susceptibility pattern...
May 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28656386/performance-of-microbial-identification-by-maldi-tof-ms-and-susceptibility-testing-by-vitek-2-from-positive-blood-cultures-after-minimal-incubation-on-solid-media
#7
S L Mitchell, K Alby
Bloodstream infections (BSIs) are a leading cause of patient morbidity and mortality. Rapid identification of organisms from BSIs is critical for initiating targeted antimicrobial therapy. Although many methods exist for rapid identification, they do not provide detailed or definitive susceptibility information. We assessed the utility of both the VITEK MS and Bruker Biotyper MALDI-TOF mass spectrometers to identify organisms from a positive blood culture bottle after only 4 h of growth on solid media compared to identification from overnight growth using the VITEK MS...
June 28, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28643493/identification-of-viridans-streptococci-with-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-by-an-in-house-method-and-a-commercially-available-system
#8
Catalina Suzana Stingu, Klaus Eschrich, Juliane Thiel, Toralf Borgmann, Reiner Schaumann, Arne C Rodloff
Two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS)-based methods were compared for their ability to identify viridans streptococci. One approach employed a reference database and software developed in-house. All inhouse measurements were performed using an Autoflex II Instrument (Bruker Daltonics GmbH, Germany). The other system, a VITEK-MS (BioMérieux, France) was operated on the commercially available V2.0 Knowledge Base for Clinical Use database. Clinical isolates of viridans streptococci (n=184) were examined...
September 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28630686/fecal-carriage-of-extended-spectrum-%C3%AE-lactamase-and-carbapenemase-producing-enterobacteriaceae-in-egyptian-patients-with-community-onset-gastrointestinal-complaints-a-hospital-based-cross-sectional-study
#9
H M Abdallah, N Alnaiemi, E A Reuland, B B Wintermans, A Koek, A M Abdelwahab, A Samy, K W Abdelsalam, C M J E Vandenbroucke-Grauls
OBJECTIVES: The aim of this study was to determine the prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase production among Enterobacteriaceae isolated from ambulatory patients with gastrointestinal complaints admitted to El-Ahrar General Hospital, Zagazig, Egypt in the period between January 2013 and May 2013. METHODS: One hundred and thirteen Enterobacteriaceae isolates were recovered from 100 consecutive Egyptian patients with community-onset gastrointestinal complaints...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28611406/targeted-next-generation-sequencing-of-the-16s-23s-rrna-region-for-culture-independent-bacterial-identification-increased-discrimination-of-closely-related-species
#10
Artur J Sabat, Evert van Zanten, Viktoria Akkerboom, Guido Wisselink, Kees van Slochteren, Richard F de Boer, Ron Hendrix, Alexander W Friedrich, John W A Rossen, Anna M D Mirjam Kooistra-Smid
The aim of this study was to develop an easy-to-use culture-free diagnostic method based on next generation sequencing (NGS) of PCR amplification products encompassing whole 16S-23S rRNA region to improve the resolution of bacterial species identification. To determine the resolution of the new method 67 isolates were subjected to four identification methods: Sanger sequencing of the 16S rRNA gene; NGS of the 16S-23S rRNA region using MiSeq (Illumina) sequencer; Microflex MS (Bruker) and VITEK MS (bioMérieux)...
June 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28597394/invasive-candidiasis-in-brescia-italy-analysis-of-species-distribution-and-antifungal-susceptibilities-during-seven-years
#11
M A De Francesco, G Piccinelli, M Gelmi, F Gargiulo, G Ravizzola, G Pinsi, L Peroni, C Bonfanti, A Caruso
The aims of this study were to evaluate the epidemiology of nosocomial candidemia in a large teaching hospital in Brescia, Italy, and the in vitro antifungal susceptibility of isolates. We analyzed 196 isolates causing fungemia in patients admitted in our hospital, between January 2009 and December 2015. Strains were identified by VITEK 2 and MALDI-TOF MS. MICs were determined by Sensititre Yeast OneTM. The resistance was defined by using the revised CLSI breakpoints/epidemiological cutoff values to assign susceptibility or wild type to systemic antifungal agents...
June 8, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28581267/characterization-of-oropharyngeal-carriage-isolates-of-neisseria-meningitidis-in-healthy-korean-adolescents-in-2015
#12
Han Wool Kim, Soyoung Lee, Daeho Kwon, Jihei Cha, Jong Gyun Ahn, Kyung Hyo Kim
The meningococcus carriage rate is age-dependent, with a high prevalence in adolescents and young adults. This cross-sectional study aimed to estimate the oropharyngeal carriage rate of meningococcus among healthy Korean adolescents and its relationship with several population characteristics. The survey was conducted from April to May 2015 among 1,460 first-year high-school students in 9 high schools located in Gyeonggi province, Korea. Each student answered a short questionnaire assessing risk factors for carriage, and posterior pharyngeal wall swab samples were obtained...
July 2017: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/28566082/-femoral-hemodialysis-catheter-related-bacteremia-due-to-globicatella-sanguinis-challenges-in-species-identification
#13
Elif Aktaş, Nafia Canan Gürsoy, Tamer Sakacı, Yener Koç, Aziz Ahmad Hamidi, Emin Bulut, Duygu Erdemir, Barış Otlu
In this case, catheter-related bacteremia due to Globicatella sanguinis in a 43 years old female patient undergoing hemodialysis with the diagnosis of diabetic nephropathy was presented and the methods in the laboratory diagnosis of the rare opportunistic pathogen, Globicatella cins, were nvestigated. A set of peripheral blood cultures and simultaneous catheter culture was obtained from the patient in third of May 2016. Biochemical tests, Phoenix (Becton Dickinson, USA) and MicroScan (Beckman Coulter, USA) automated systems and matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF MS) based Microflex MS (Bruker, Daltonics, Germany) and VITEK MS (database v2...
April 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28566081/-a-rarely-isolated-bacterium-in-microbiology-laboratories-streptococcus-uberis
#14
Canan Eryıldız, Şebnem Bukavaz, Şaban Gürcan, Osman Hatipoğlu
Streptococcus uberis is a gram-positive bacterium that is mostly responsible for mastitis in cattle. The bacterium rarely has been associated with human infections. Conventional phenotyphic methods can be inadequate for the identification of S.uberis; and in microbiology laboratories S.uberis is confused with the other streptococci and enterococci isolates. Recently, molecular methods are recommended for the accurate identification of S.uberis isolates. The aim of this report is to present a lower respiratory tract infection case caused by S...
April 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28545528/comparison-of-biomarker-based-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-and-conventional-methods-in-the-identification-of-clinically-relevant-bacteria-and-yeast
#15
Ali Kassim, Valentin Pflüger, Zul Premji, Claudia Daubenberger, Gunturu Revathi
BACKGROUND: MALDI-TOF MS is an analytical method that has recently become integral in the identification of microorganisms in clinical laboratories. It relies on databases that majorly employ pattern recognition or fingerprinting. Biomarker based databases have also been developed and there is optimism that these may be superior to pattern recognition based databases. This study compared the performance of ribosomal biomarker based MALDI-TOF MS and conventional methods in the identification of selected bacteria and yeast...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28542393/head-to-head-comparison-of-microflex-lt-and-vitek-ms-systems-for-routine-identification-of-microorganisms-by-maldi-tof-mass-spectrometry-in-chile
#16
Lorena Porte, Patricia García, Stephanie Braun, María Teresa Ulloa, Mónica Lafourcade, Alisson Montaña, Carolina Miranda, Gerardo Acosta-Jamett, Thomas Weitzel
BACKGROUND: Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) is a new and revolutionary identification method for microorganisms and has recently been introduced into clinical microbiology in many industrialized countries in Europe and North America. OBJECTIVES: Our study aimed to compare the performance and practicality of two commercial MALDI-TOF MS platforms in a head-to head manner at a routine laboratory in Chile...
2017: PloS One
https://www.readbyqxmd.com/read/28539340/evaluating-and-improving-vitek-ms-for-identification-of-clinically-relevant-species-of-trichosporon-and-the-closely-related-genera-cutaneotrichosporon-and-apiotrichum
#17
João N de Almeida, Viviane M Favero Gimenes, Elaine C Francisco, Lumena P Machado Siqueira, Renato K Gonçalves de Almeida, Juliette Guitard, Christophe Hennequin, Arnaldo L Colombo, Gil Benard, Flavia Rossi
Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immunocompromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trichosporon taxonomy has been reassessed, and three genera have been proposed for the pathogenic species: Trichosporon, Cutaneotrichosporon, and Apiotrichum Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has replaced old phenotypic methods for microorganism identification in clinical laboratories, but spectral profile databases have to be evaluated and improved for optimal species identification performance...
August 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28490491/comparison-of-the-vitek-ms-and-bruker-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-systems-for-identification-of-rhodococcus-equi-and-dietzia-spp
#18
Carlos Ruiz de Alegría Puig, Lilian Pilares, Francesc Marco, Jordi Vila, Luis Martínez-Martínez, Jesús Navas
Rhodococcus equi causes pyogranulomatous pneumonia in domesticated animals and immunocompromised humans. Dietzia spp. are environmental bacteria that have rarely been associated with human infections. R. equi and Dietzia spp. are closely related actinomycetes. Phenotypic discrimination between R. equi and Dietzia on the basis of their Gram stain morphology and colony appearance is problematic. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a fast, reliable, and cost-effective method for identification of a wide variety of microorganisms...
July 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28424252/comparison-of-saramis-4-12-and-ivd-3-0-vitek-ms-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-identification-of-mycobacteria-from-solid-and-liquid-culture-media
#19
Caroline Leyer, Guillaume Gregorowicz, Faiza Mougari, Laurent Raskine, Emmanuelle Cambau, Dominique de Briel
During the last decade, many investigators have studied matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification of mycobacteria. Diverse and contradictory results indicated that optimal level for routine testing has not been reached yet. This work aimed to assess Vitek MS through two distinct versions, Saramis v4.12 RUO and the IVD v3.0, under conditions close to routine laboratory practice. Overall, 111 mycobacterial isolates were subjected to protein extraction and same spectra were matched against both databases...
July 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28348803/non-o1-non-o139-vibrio-cholerae-septicaemia-in-a-saudi-man-a-case-report
#20
Reham Kaki, Dalia El-Hossary, Asif Jiman-Fatani, Rahaf Al-Ghamdi
Background. The non-O1/non-O139 serogroups of Vibrio cholerae occur in diverse natural niches, and usually cause mild and self-limiting gastrointestinal illness. However, they have well-documented potential to cause invasive and extra-intestinal infections among immunocompromised patients. Furthermore, their ability to grow in low-salinity surface water, and the existence of asymptomatic human carriers, suggest novel acquisition routes for this unusual infection, even in people without obvious risk factors...
February 2017: JMM Case Reports
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