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https://www.readbyqxmd.com/read/29656124/development-of-an-improved-rapid-bacpro%C3%A2-protocol-and-a-method-for-direct-identification-from-blood-culture-positive-bottles-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#1
Takatoshi Yonezawa, Tomohisa Watari, Kazuho Ashizawa, Daisuke Hanada, Takako Yanagiya, Naoki Watanabe, Takashi Terada, Yutaka Tomoda, Satoshi Fujii
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been incorporated into pathogenic bacterial identification methods and has improved their rapidity. Various methods have been reported to directly identify bacteria with MALDI-TOF MS by pretreating culture medium in blood culture bottles. Rapid BACpro® (Nittobo Medical Co., Ltd.) is a pretreatment kit for effective collection of bacteria with cationic copolymers. However, the Rapid BACpro® pretreatment kit is adapted only for MALDI Biotyper (Bruker Daltonics K...
April 12, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29643203/evaluation-of-the-vitek-ms-v3-0-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-system-for-identification-of-mycobacterium-and-nocardia-species
#2
Barbara A Body, Melodie A Beard, E Susan Slechta, Kimberly E Hanson, Adam P Barker, N Esther Babady, Tracy McMillen, Yi-Wei Tang, Barbara A Brown-Elliott, Elena Iakhiaeva, Ravikiran Vasireddy, Sruthi Vasireddy, Terry Smith, Richard J Wallace, S Turner, L Curtis, Susan Butler-Wu, Jenna Rychert
This multi-center study was designed to assess the accuracy and reproducibility of the VITEK MS v3.0 matrix-assisted laser desorption ionization--time of flight (MALDI-TOF) mass spectrometry system for identification of Mycobacterium and Nocardia species compared to DNA sequencing. A total of 963 clinical isolates representing 51 taxa were evaluated. In all, 663 isolates were correctly identified to the species level (69%) with another 231 (24%) correctly identified to the complex or group level. Fifty-five isolates (6%) could not be identified despite repeat testing...
April 11, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29628352/-identification-of-yeasts-from-commercial-atti%C3%A3-k%C3%A3-in-abidjan-c%C3%A3-te-d-ivoire-preliminary-study
#3
C G Kouadio-Yapo, G S P Dou, N A D Aka, K D Zika, K D Adoubryn, M Dosso
OBJECTIVE: This study aims to identify yeasts from commercial attiéké. PATIENTS AND METHODS: This descriptive cross-sectional survey involving 103 samples of attiéké sold on the market from August to September 2017. It was conducted from the mycology laboratory of the Institut Pasteur of Côte d'Ivoire. The samples were seeded on YGC medium. The colonies of positive culture were counted and seeded again on chromogen medium. Identification of yeasts species was carried out by MALDI-TOF mass spectrometry (Vitek MS bioMérieux)...
April 6, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29616003/simple-sample-preparation-method-for-direct-microbial-identification-and-susceptibility-testing-from-positive-blood-cultures
#4
Hong-Wei Pan, Wei Li, Rong-Guo Li, Yong Li, Yi Zhang, En-Hua Sun
Rapid identification and determination of the antibiotic susceptibility profiles of the infectious agents in patients with bloodstream infections are critical steps in choosing an effective targeted antibiotic for treatment. However, there has been minimal effort focused on developing combined methods for the simultaneous direct identification and antibiotic susceptibility determination of bacteria in positive blood cultures. In this study, we constructed a lysis-centrifugation-wash procedure to prepare a bacterial pellet from positive blood cultures, which can be used directly for identification by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and antibiotic susceptibility testing by the Vitek 2 system...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29573441/contemporary-microbiology-and-identification-of-corynebacteria-spp-causing-infections-in-human
#5
REVIEW
Aleksandra Anna Zasada, Ewa Mosiej
The Corynebacterium genus are bacteria of growing clinical importance. It is because of progress in medicine that result in growing population of immunocompromised patients and growing number of infections caused by opportunistic pathogens. A new infections caused by Corynebacterium species regarded previously as commensal microorganisms as well as infections caused by new Corynebacterium species are described. Parallel with changes in Corynebacteria infections the microbiological laboratory diagnostic possibilities are changing...
March 24, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29513850/matrix-assisted-laser-desorption-ionization-time-of-flight-a-promising-alternative-method-of-identifying-the-major-coagulase-negative-staphylococci-species
#6
Lucas Eduardo Trevisoli, Larissa Bail, Luiza Souza Rodrigues, Danieli Conte, Jussara Kasuko Palmeiro, Libera Maria Dalla-Costa
INTRODUCTION.: This study aimed to evaluate different methods for differentiation of species of coagulase-negative staphylococci (CoNS) that caused infections in hospitalized immunocompromised patients. METHODS.: A total of 134 CoNS strains were characterized using four different methods. RESULTS.: The results of matrix assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) analysis were in complete agreement with those of tuf gene sequencing (kappa index = 1...
January 2018: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/29492868/rapid-and-robust-maldi-tof-ms-techniques-for-microbial-identification-a-brief-overview-of-their-diverse-applications
#7
REVIEW
Kyoung-Soon Jang, Young Hwan Kim
Advances in mass spectrometry have enabled the investigation of various biological systems by directly analyzing diverse sets of biomolecules (i.e., proteins, lipids, and carbohydrates), thus making a significant impact on the life sciences field. Over the past decade, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been widely utilized as a rapid and reliable method for the identification of microorganisms. MALDI-TOF MS has come into widespread use despite its relatively low resolving power (full width at half maximum, FWHM: < 5,000) and its incompatibility with tandem MS analysis, features with which other high-resolution mass spectrometers are equipped...
February 28, 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29467194/identification-and-characterization-of-clostridium-difficile-genotype-st37-by-maldi-tof-mass-spectrometry
#8
Ruxin Li, Di Xiao, Jing Yang, Suju Sun, Samuel Kaplan, Zhirong Li, Yanan Niu, Cuixin Qiang, Yu Zhai, Xiaoming Wang, Xingzhen Zhao, Baoxin Zhao, Martin Welker, David H Pincus, Dazhi Jin, Mini Kamboj, Guanghui Zheng, Guojun Zhang, Jianzhong Zhang, Yi-Wei Tang, Jianhong Zhao
Clostridium difficile multilocus sequence type 37 (ST37), which mainly corresponds to ribotype 017, has been a dominant genotype circulating in China. In this study, we report the use of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to analyze and characterize 204 C. difficile clinical isolates including 49 ST37 and 155 non-ST37 isolates collected in China and other countries. Distribution of two major protein peaks ( m/z 3242 and 3286) was significantly different between ST37 and non-ST37 prototype strains and clinical isolates...
February 21, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29447913/microbiological-surveillance-of-plasmid-mediated-colistin-resistance-in-human-enterobacteriaceae-isolates-in-romagna-northern-italy-august-2016-july-2017
#9
F Del Bianco, M Morotti, M F Pedna, P Farabegoli, V Sambri
OBJECTIVES: To start a surveillance program to investigate the possible diffusion of mobilized colistin resistance genes in Enterobacteriaceae strains isolated in the Unit of Microbiology of the Great Romagna Hub Laboratory. METHODS: All the colistin resistant Enterobacteriaceae, isolated from August 1st 2016 to July 31st 2017, were prospectively evaluated for mcr-1 a mcr-2. Backdated survey of mcr-3, mcr-4 and mcr-5 was performed on the same group of isolates. Species identification was achieved by Vitek MS and the antibiotic susceptibility testing was performed both with Vitek-2 and Sensititre systems...
February 12, 2018: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29434578/oligonucleotide-array-and-vitek-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-in-species-identification-of-blood-yeast-isolates
#10
Ming-Chi Li, Tsung C Chang, Hung-Mo Chen, Chi-Jung Wu, Shu-Li Su, Susan S-J Lee, Po-Lin Chen, Nan-Yao Lee, Ching-Chi Lee, Chia-Wen Li, Ling-Shan Syue, Wen-Chien Ko
We evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry using VITEK MS (IVD database) and an oligonucleotide array based on the internal transcribed spacer-1 (ITS-1) and ITS-2 sequences of rRNA genes for the identification of Candida spp. from blood cultures. Five-hundred and twelve consecutive bloodstream yeast isolates were collected daily and initially identified by the phenotypic automated method (VITEK YBC or VITEK2 YST card). Inconsistent results were confirmed by D1-D2 region of 28S rRNA genes and ITSs...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29401558/same-day-identification-and-antimicrobial-susceptibility-testing-of-bacteria-in-positive-blood-culture-broths-using-short-term-incubation-on-solid-medium-with-the-microflex-lt-vitek-ms-and-vitek2-systems
#11
Jihye Ha, Sung Kuk Hong, Geum Hee Han, Myungsook Kim, Dongeun Yong, Kyungwon Lee
BACKGROUND: Early and appropriate antibiotic treatment improves the clinical outcome of patients with septicemia; therefore, reducing the turn-around time for identification (ID) and antimicrobial susceptibility test (AST) results is essential. We established a method for rapid ID and AST using short-term incubation of positive blood culture broth samples on solid media, and evaluated its performance relative to that of the conventional method using two rapid ID systems and a rapid AST method...
May 2018: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/29394275/rapid-identification-of-mycobacteria-from-positive-mgit-broths-of-primary-cultures-by-maldi-tof-mass-spectrometry
#12
Tsi-Shu Huang, Chia-Chien Lee, Hui-Zin Tu, Susan Shin-Jung Lee
BACKGROUND: Rapid identification of mycobacteria is important for timely treatment and the implementation of public health measures. The MGIT system ensures rapid detection of mycobacteria, but identification is usually delayed by days to weeks due to further subculture on solid medium. Matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to effectively identify mycobacteria isolates subcultured from solid or liquid media. Reports of identification directly from MGIT broths of both sterile and non-sterile clinical specimens, omitting the subculture step, were limited and not satisfactory before...
2018: PloS One
https://www.readbyqxmd.com/read/29391258/identification-of-clostridium-species-using-the-vitek%C3%A2-ms
#13
Lucia Sanchez Ramos, Arne C Rodloff
The genus Clostridium is of high clinical relevance, as some species may cause rapid and even lethal infections. Thus, a timely identification of these anaerobic bacteria is desirable. Conventional identification methods rely on biochemical properties of these organisms, however, establishing these is time-consuming and not always reliable. Alternatively, 16S rRNA gene sequence based diagnostic methods may be used, but they are expensive and not ubiquitously available. This study was designed to assess the possibility to identify Clostridium species employing the matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS)...
January 29, 2018: Anaerobe
https://www.readbyqxmd.com/read/29345117/candida-auris-a-systematic-review-and-meta-analysis-of-current-updates-on-an-emerging-multidrug-resistant-pathogen
#14
John Osei Sekyere
From 2009, Candida auris has emerged as a multidrug-resistant ascomycete yeast pathogen with the capacity for easy transmission between patients and hospitals, as well as persistence on environmental surfaces. Its association with high mortalities, breakthrough and persistent candidaemia, inconsistencies in susceptibility testing results, misidentification by available commercial identification systems and treatment failure, complicates its management and detection. Within the last nine years, C. auris has been increasingly reported from far-Eastern Asia, the Middle East, Africa, Europe, South and North America with substantial fatalities and misidentification...
January 18, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/29228397/yeast-identification-by-sequencing-biochemical-kits-maldi-tof-ms-and-rep-pcr-dna-fingerprinting
#15
Ying Zhao, Chi-Ching Tsang, Meng Xiao, Jasper F W Chan, Susanna K P Lau, Fanrong Kong, Yingchun Xu, Patrick C Y Woo
No study has comprehensively evaluated the performance of 28S nrDNA and ITS sequencing, commercial biochemical test kits, MALDI-TOF MS platforms, and the emerging rep-PCR DNA fingerprinting technology using a cohort of yeast strains collected from a clinical microbiology laboratory. In this study, using 71 clinically important yeast isolates (excluding Candida albicans) collected from a single centre, we determined the concordance of 28S nrDNA and ITS sequencing and evaluated the performance of two commercial test kits, two MALDI-TOF MS platforms, and rep-PCR DNA fingerprinting...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29221301/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-a-powerful-tool-for-identification-of-corynebacterium-species
#16
Rong Bao, Xiaodong Gao, Bijie Hu, Zhaoyan Zhou
Background: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a powerful tool that has initiated a revolution in the clinical microbiology laboratory for identification of nosocomial pathogens. The efficacy of MALDI-TOF MS produced by bioMerieux, Marcy l'Etoile, France (VITEK MS) for detecting Corynebacterium remains unknown. Methods: Corynebacterium isolates were isolated from clinical specimen in a tertial teaching hospital from 2012 to 2013...
September 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29192600/magazines-in-waiting-areas-of-hospital-a-forgotten-microbial-reservoir
#17
Mathias Adé, Sandrine Burger, Anaelle Cuntzmann, Julien Exinger, Olivier Meunier
The hospital environment is a potential source of microbial contamination. Thus, the magazines in hospital's waiting rooms are handled by patients and visitors whose health and hygiene conditions can vary widely. In this context, we had measured the microbial load on the surface of magazines. Fifteen magazines from 5 waiting rooms of hospital are sampled by agar prints at the areas taken in hand. The agar plates are incubated at 30̊C for 72h. The colonies are counted and identified by MALDI-TOF mass spectrometry (Vitek® -MS)...
December 1, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/29191475/evaluation-of-bruker-biotyper-and-vitek-ms-for-the-identification-of-candida-tropicalis-on-different-solid-culture-media
#18
He Wang, Ying Li, Xin Fan, Tzong-Shi Chiueh, Ying-Chun Xu, Po-Ren Hsueh
BACKGROUND: The aim of this study was to investigate the performance of the Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and Vitek MS systems for identification of genetically-confirmed blood isolates of Candida tropicalis that had been grown on several types of culture media commonly used for primary fungal isolation. METHODS: Isolates included 105 from the National China Hospital Invasive Fungal Surveillance Net program (CHIF-NET) and 120 from National Taiwan University Hospital (NTUH)...
November 11, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29158151/a-novel-liquid-media-mycobacteria-extraction-method-for-maldi-tof-ms-identification-using-vitek%C3%A2-ms
#19
Erik Moreno, Eric Miller, Elizabeth Miller, Heather Totty, Parampal Deol
A low-cost identification method that can be performed directly from a positive liquid medium culture is needed for the diagnosis of mycobacterial infections. Here, we describe a novel, cost-effective, and validated method that allows for direct and rapid identification of mycobacteria from a positive liquid culture using VITEK® MS with a total process duration under 45min. From a liquid mycobacteria culture a 3.0mL aliquot is removed 24-72h post positivity and centrifuged to create a pellet. After decanting, the tube is blotted dry, the pellet is re-suspended in 0...
January 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29142047/multicenter-evaluation-of-the-vitek-ms-v3-0-system-for-the-identification-of-filamentous-fungi
#20
Jenna Rychert, E Sue Slechta, Adam P Barker, Edwin Miranda, N Esther Babady, Yi-Wei Tang, Connie Gibas, Nathan Wiederhold, DeAnna Sutton, Kimberly E Hanson
Invasive fungal infections are an important cause of morbidity and mortality affecting primarily immunocompromised patients. While fungal identification to the species level is critical to providing appropriate therapy, it can be slow and laborious and often relies on subjective morphological criteria. The use of matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry has the potential to speed up and improve the accuracy of identification. In this multicenter study, we evaluated the accuracy of the Vitek MS v3...
February 2018: Journal of Clinical Microbiology
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