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https://www.readbyqxmd.com/read/28264610/corynebacterium-species-causing-breast-abscesses-among-patients-attending-a-tertiary-care-hospital-in-chennai-south-india
#1
Indira Poojary, Ann Kurian, Jayalekshmi V A, Debora Devapriya J, Thirunarayan M A
BACKGROUND: Corynebacterium species other than Corynebacterium diphtheriae were mostly considered contaminants in the past, but there are reports of their association with wide variety of human infections lately. In this study, we look into Corynebacterium species isolated from breast abscess patients and assess their antimicrobial susceptibility pattern and treatment outcomes. MATERIAL AND METHODS: Pus samples from suspected breast abscess cases were examined from October 2014 to September 2015...
March 6, 2017: Infectious Diseases
https://www.readbyqxmd.com/read/28262370/species-identification-of-streptococcus-bovis-group-isolates-causing-bacteremia-a-comparison-of-two-maldi-tof-ms-systems
#2
Charlotte N Agergaard, Elisa Knudsen, Rimtas Dargis, Xiaohui C Nielsen, Jens J Christensen, Ulrik S Justesen
This study compared two MALDI-TOF MS systems (Biotyper and VITEK MS) on clinical Streptococcus bovis group isolates (n=66). The VITEK MS gave fewer misidentifications and a higher rate of correct identifications than the Biotyper. Only the identification of S. lutetiensis by the VITEK MS was reliable. Additional optimization of the available system databases is needed.
February 20, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28254444/comparison-of-growth-on-mannitol-salt-agar-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-vitek-%C3%A2-2-with-partial-sequencing-of-16s-rrna-gene-for-identification-of-coagulase-negative-staphylococci
#3
Funmilola A Ayeni, Camilla Andersen, Niels Nørskov-Lauritsen
Mannitol salt agar (MSA) is often used in resources' limited laboratories for identification of S. aureus however, coagulase-negative staphylococci (CoNS) grows and ferments mannitol on MSA. 171 strains of CoNS which have been previously misidentified as S. aureus due to growth on MSA were collected from different locations in Nigeria and two methods for identification of CoNS were compared i.e. ViTEK 2 and MALDI-TOF MS with partial 16S rRNA gene sequencing as gold standard. Partial tuf gene sequencing was used for contradicting identification...
February 28, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28224768/comparison-of-the-bruker-biotyper-and-vitek-ms-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-systems-using-a-formic-acid-extraction-method-to-identify-common-and-uncommon-yeast-isolates
#4
COMPARATIVE STUDY
Hyun Seung Lee, Jong Hee Shin, Min Ji Choi, Eun Jeong Won, Seung Jung Kee, Soo Hyun Kim, Myung Geun Shin, Soon Pal Suh
BACKGROUND: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows rapid and accurate identification of clinical yeast isolates. In-tube formic acid/acetonitrile (FA/ACN) extraction is recommended prior to the analysis with MALDI Biotyper, but the direct on-plate FA extraction is simpler. We compared the Biotyper with the VITEK MS for the identification of various clinically relevant yeast species, focusing on the use of the FA extraction method...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28209347/high-resolution-mass-spectrometry-based-method-applicable-for-a-wide-range-of-3-hydroxy-3-methyl-glutaryl-coenzyme-a-reductase-inhibitors-in-blood-serum-including-intermediates-and-products-of-the-cholesterol-biosynthetic-pathway
#5
Vít Kosek, Milena Stránská, Marie Fenclová, Tomáš Ruml, Libor Vítek, Jana Hajšlová
Statins belong to the major class of hypolipidemic drugs. They act as competitive inhibitors of 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-CoA) reductase, a rate-limiting enzyme in the cholesterol biosynthetic pathway. This inhibition not only leads to the depletion of cholesterol and its fatty acid esters, but also to the depletion of the intermediates of this metabolic pathway (mainly pyrophosphates), which can play an important role in tumor proliferation. The aim of the current study was to establish a versatile multi-analyte method capable of quantitative determination of various currently-used statins, together with free cholesterol (FC), cholesterol esters (CEs), and some key intermediates of the mevalonate pathway occurring in human serum...
March 17, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28154553/identification-and-antifungal-susceptibility-profiles-of-candida-nivariensis-and-candida-bracarensis-in-a-multi-center-chinese-collection-of-yeasts
#6
Xin Hou, Meng Xiao, Sharon C-A Chen, He Wang, Shu-Ying Yu, Xin Fan, Fanrong Kong, Ying-Chun Xu
Candida nivariensis and C. bracarensis are two emerging cryptic species within the C. glabrata complex. Thirteen of these isolates from 10 hospitals in China were studied for their species identification and antifungal susceptibilities. Phenotypic and molecular [rDNA ITS sequencing, D1/D2 sequencing and ITS sequencer-based capillary gel electrophoresis (SCGE)] and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS identification methods were compared for their performance in species identification...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28131716/nosocomial-fungemia-by-candida-auris-first-four-reported-cases-in-continental-europe
#7
Alba Cecilia Ruiz Gaitán, Ana Moret, José Luis López Hontangas, José Miguel Molina, Ana Isabel Aleixandre López, Alicia Hernández Cabezas, Juan Mollar Maseres, Rabat Chouman Arcas, María Dolores Gómez Ruiz, Miguel Ángel Chiveli, Emilia Cantón, Javier Pemán
BACKGROUND: Candida auris is an emerging multidrug-resistant yeast that can cause invasive infections and is associated with high mortality. It is typically resistant to fluconazole and voriconazole and, some cases, also to echinocandins and amphotericin B. This species, phylogenetically related to Candida haemulonii, is frequently misidentified by commercial identification techniques in clinical laboratories; therefore, the real prevalence of C. auris infections may be underestimated...
January 25, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/28124968/-accurate-diagnosis-of-pseudomonas-luteola-in-routine-microbiology-laboratory-on-the-occasion-of-two-isolates
#8
Muharrem Çiçek, Gülşen Hasçelik, H Kaan Müştak, K Serdar Diker, Burçin Şener
Pseudomonas luteola which was previously known as Chryseomonas luteola; is a gram-negative, non-fermentative, aerobic, motile, non-spore-forming bacillus. It is frequently found as a saprophyte in soil, water and other damp environments and is an opportunistic pathogen in patients with underlying medical disorders or with indwelling catheters. It has been reported as an uncommon cause of bacteremia, sepsis, septic arthritis, meningitis, endocarditis, and peritonitis. Thus, early and accurate identification of this rare species is important for the treatment and also to provide information about the epidemiology of P...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28116553/performance-of-the-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-system-for-rapid-identification-of-streptococci-a-review
#9
REVIEW
W-T Fan, T-T Qin, R-R Bi, H-Q Kang, P Ma, B Gu
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been developed as a new type of soft ionization mass spectrometry in recent years. An increasing number of clinical microbiological laboratories consider it as an innovative approach for bacterial identification. This study was undertaken in order to evaluate the use of MALDI-TOF MS for rapid identification of the clinical streptococci. A systematic review was conducted based on a literature search of the Medline and Embase databases...
January 23, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27990823/abrf-proteome-informatics-research-group-iprg-2015-study-detection-of-differentially-abundant-proteins-in-label-free-quantitative-lc-ms-ms-experiments
#10
Meena Choi, Zeynep F Eren-Dogu, Christopher Colangelo, John Cottrell, Michael R Hoopmann, Eugene A Kapp, Sangtae Kim, Henry Lam, Thomas A Neubert, Magnus Palmblad, Brett S Phinney, Susan T Weintraub, Brendan MacLean, Olga Vitek
Detection of differentially abundant proteins in label-free quantitative shotgun liquid chromatography-tandem mass spectrometry (LC-MS/MS) experiments requires a series of computational steps that identify and quantify LC-MS features. It also requires statistical analyses that distinguish systematic changes in abundance between conditions from artifacts of biological and technical variation. The 2015 study of the Proteome Informatics Research Group (iPRG) of the Association of Biomolecular Resource Facilities (ABRF) aimed to evaluate the effects of the statistical analysis on the accuracy of the results...
January 3, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/27987046/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-for-rapid-identification-of-micro-organisms-in-the-routine-clinical-microbiology-laboratory
#11
C Wattal, J K Oberoi, N Goel, R Raveendran, S Khanna
The study evaluates the utility of matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) Vitek MS for identification of microorganisms in the routine clinical microbiology laboratory. From May 2013 to April 2014, microbial isolates recovered from various clinical samples were identified by Vitek MS. In case of failure to identify by Vitek MS, the isolate was identified using the Vitek 2 system (bioMerieux, France) and serotyping wherever applicable or otherwise by nucleic acid-mediated methods...
December 16, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27955639/control-of-carbapenem-resistant-acinetobacter-baumannii-outbreak-in-an-intensive-care-unit-of-a-teaching-hospital-in-southern-italy
#12
Aida Bianco, Angela Quirino, Mariavalentina Giordano, Vito Marano, Claudia Rizzo, Maria Carla Liberto, Alfredo Focà, Maria Pavia
BACKGROUND: Acinetobacter baumannii is an opportunistic pathogen that has become a major cause of concern, since it is a frequent cause of healthcare-associated infections (HAIs). The aim of the study was to describe the occurrence, the management and the control of an outbreak that occurred in an intensive care unit (ICU) of a teaching hospital in Southern Italy caused by multiple strains of extensively drug-resistant A. baumannii (XDRAB). METHODS: Case-patient was defined as a patient with an healthcare-associated infection caused by an XDRAB isolate identified in a clinically significant culture...
December 12, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27934842/infections-related-to-granulicatella-adiacens-report-of-two-cases-and-review-of-literature
#13
REVIEW
S Macin, A Ç İnkaya, Ö Tuncer, S Ünal, Y Akyön
Infections due to nutritionally variant streptococci are diagnosed rarely due to difficulties encountered during identification and isolation. Mortality rate in these infections is high therefore appropriate supplemented media and reliable detection systems should be implemented to isolate these fastidious organisms. Here, we describe two cases of Granulicatella adiacens infections. All microbiologic identifications were made with MALDI-TOF Vitek MS (BioMerieux, France), and the results confirmed by 16S ribotyping...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27847376/relative-prevalence-and-antimicrobial-susceptibility-of-clinical-isolates-of-elizabethkingia-species-based-on-16s-rrna-gene-sequencing
#14
Mi-Soon Han, Hyunsoo Kim, Yangsoon Lee, Myungsook Kim, Nam Su Ku, Jun Yong Choi, Dongeun Yong, Seok Hoon Jeong, Kyungwon Lee, Yunsop Chong
Some of the previously reported clinical isolates of Elizabethkingia meningoseptica may be later named species of Elizabethkingia We determined the accuracy of species identification (with two matrix-assisted laser desorption ionization-time of flight mass spectrometry [MALDI-TOF MS] systems and the Vitek 2 GN card), relative prevalence of three Elizabethkingia spp. in clinical specimens, and antimicrobial susceptibility of the species identified by 16S rRNA gene sequencing. Specimens for culture were collected from patients in a university hospital in Seoul, South Korea, between 2009 and 2015...
January 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27802877/routine-identification-of-nocardia-species-by-maldi-tof-mass-spectrometry
#15
Victoria Girard, Sandrine Mailler, Sophie Polsinelli, Delphine Jacob, Marie Christine Saccomani, Beatrice Celliere, Valerie Monnin, Alex van Belkum, Ferry Hagen, Jacques F Meis, Géraldine Durand
We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) database leads to 94% accurate identification to the species level on a set of 164 isolates. The possibility to identify Nocardia spp. using MALDI-TOF MS will be available in the next release of VITEK MS update (IVD Version 3...
January 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27796646/streptococcus-bovis-new-taxonomy-does-subspecies-distinction-matter
#16
E Ben-Chetrit, Y Wiener-Well, L Kashat, A M Yinnon, M V Assous
Bacteremia with Streptococcus bovis/equinus complex strains is associated with hepatobiliary disease, colorectal lesions (CL), and infective endocarditis (IE). This study addressed the clinical significance of subspecies distinction of previously designated S. bovis blood culture isolates according to the updated nomenclature. During 2002-2013, all blood culture isolates previously designated as S. bovis were recultured and identified using 16S rRNA gene sequencing and MALDI-TOF (Bruker BioTyper and Vitek MS, bioMérieux)...
February 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27771394/bacteroides-pyogenes-causing-serious-human-wound-infection-from-animal-bites
#17
Jillian S Y Lau, Tony M Korman, Alex Yeung, Richard Streitberg, Michelle J Francis, Maryza Graham
Bacteroides pyogenes is part of the normal oral flora of domestic animals. There is one previous report of human infection, with B. pyogenes bacteremia following a cat bite (Madsen 2011). We report seven severe human infections where B. pyogenes was identified by Bruker matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDTI-TOF MS), but not by VITEK MS and was misidentified by VITEK ANC card.
December 2016: Anaerobe
https://www.readbyqxmd.com/read/27750157/nosocomial-outbreak-of-extended-spectrum-%C3%AE-lactamase-producing-enterobacter-ludwigii-co-harbouring-ctx-m-8-shv-12-and-tem-15-in-a-neonatal-intensive-care-unit-in-venezuela
#18
Ana Flores-Carrero, Indira Labrador, Alberto Paniz-Mondolfi, David R Peaper, Dana Towle, María Araque
Enterobacter spp. have emerged as an important group of pathogens linked to outbreaks in neonatal intensive care units (NICUs), usually involving strains expressing extended-spectrum β-lactamases (ESBLs). The aim of this study was to describe the first nosocomial bloodstream infection outbreak caused by Enterobacter ludwigii co-harbouring CTX-M-8, SHV-12 and TEM-15 in a NICU in a Venezuelan hospital. Initial bacterial identification was achieved by VITEK(®)2 system and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (VITEK(®) MS) and was subsequently confirmed by nucleotide sequencing of the 16S rDNA gene and hsp60 genotyping...
December 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27720207/identification-antimicrobial-resistance-and-molecular-characterization-of-the-human-emerging-pathogen-streptococcus-gallolyticus-subsp-pasteurianus
#19
Giovanni Gherardi, Claudio Palmieri, Emanuela Marini, Arianna Pompilio, Valentina Crocetta, Giovanni Di Bonaventura, Roberta Creti, Bruna Facinelli
This study aimed to retrospectively identify 22Streptococcus bovis clinical strains based on the new taxonomy, as well as to investigate their antibiotic-resistance and clonality. Strains were identified by Phoenix100 system, 16S rRNA sequencing, and two MALDI-TOF MS platforms (Bruker Biotyper, Vitek MS). Antibiotic resistance was determined both phenotypically and genotypically, and clonality was assessed by PFGE. Most of strains (63.6%) were isolated from urine, and diabetes was the most common underlying disease (31...
December 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27713213/identification-of-uncommon-candida-species-using-commercial-identification-systems
#20
COMPARATIVE STUDY
Tae-Hyoung Kim, Oh Joo Kweon, Hye Ryoun Kim, Mi-Kyung Lee
Recently, several studies have revealed that commercial microbial identification systems do not accurately identify the uncommon causative species of candidiasis, including Candida famata, Meyerozyma guilliermondii, and C. auris. We investigated the accuracy of species-level identification in a collection of clinical isolates previously identified as C. famata (N = 38), C. lusitaniae (N = 1 2), and M. guilliermondii (N = 5) by the Vitek 2 system. All 55 isolates were re-analyzed by the Phoenix system (Becton Dickinson Diagnostics), two matrix-assisted laser desorption ionization-time of flight mass spectrometry analyzers (a Vitek MS and a Bruker Biotyper), and by sequencing of internal transcribed spacer (ITS) regions or 26S rRNA gene D1/D2 domains...
December 28, 2016: Journal of Microbiology and Biotechnology
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