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Vitek ms

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https://www.readbyqxmd.com/read/29192600/magazines-in-waiting-areas-of-hospital-a-forgotten-microbial-reservoir
#1
Mathias Adé, Sandrine Burger, Anaelle Cuntzmann, Julien Exinger, Olivier Meunier
The hospital environment is a potential source of microbial contamination. Thus, the magazines in hospital's waiting rooms are handled by patients and visitors whose health and hygiene conditions can vary widely. In this context, we had measured the microbial load on the surface of magazines. Fifteen magazines from 5 waiting rooms of hospital are sampled by agar prints at the areas taken in hand. The agar plates are incubated at 30̊C for 72h. The colonies are counted and identified by MALDI-TOF mass spectrometry (Vitek®-MS)...
December 1, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/29191475/evaluation-of-bruker-biotyper-and-vitek-ms-for-the-identification-of-candida-tropicalis-on-different-solid-culture-media
#2
He Wang, Ying Li, Xin Fan, Tzong-Shi Chiueh, Ying-Chun Xu, Po-Ren Hsueh
BACKGROUND: The aim of this study was to investigate the performance of the Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and Vitek MS systems for identification of genetically-confirmed blood isolates of Candida tropicalis that had been grown on several types of culture media commonly used for primary fungal isolation. METHODS: Isolates included 105 from the National China Hospital Invasive Fungal Surveillance Net program (CHIF-NET) and 120 from National Taiwan University Hospital (NTUH)...
November 11, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29158151/a-novel-liquid-media-mycobacteria-extraction-method-for-maldi-tof-ms-identification-using-vitek%C3%A2-ms
#3
Erik Moreno, Eric Miller, Elizabeth Miller, Heather Totty, Parampal Deol
A low-cost identification method that can be performed directly from a positive liquid medium culture is needed for the diagnosis of mycobacterial infections. Here, we describe a novel, cost-effective, and validated method that allows for direct and rapid identification of mycobacteria from a positive liquid culture using VITEK® MS with a total process duration under 45min. From a liquid mycobacteria culture a 3.0mL aliquot is removed 24-72h post positivity and centrifuged to create a pellet. After decanting, the tube is blotted dry, the pellet is re-suspended in 0...
November 17, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29142047/multi-center-evaluation-of-the-vitek-ms-v3-0-system-for-the-identification-of-filamentous-fungi
#4
Jenna Rychert, E Sue Slechta, Adam P Barker, Edwin Miranda, N Esther Babady, Yi-Wei Tang, Connie Gibas, Nathan Wiederhold, DeAnna Sutton, Kimberly E Hanson
Invasive fungal infections are an important cause of morbidity and mortality affecting primarily immunocompromised patients. While fungal identification to the species level is critical to providing appropriate therapy, it can be slow, laborious, and often relies on subjective morphologic criteria. The use of MALDI-TOF mass spectrometry has the potential to speed up and improve the accuracy of identification. In this multi-center study, we evaluated the accuracy of the VITEK® MS v3.0 system in identifying 1,601 clinical mold isolates as compared to identification by DNA sequence analysis and supported by morphologic and phenotypic testing...
November 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29062487/challenges-in-the-identification-of-chryseobacterium-indologenes-and-elizabethkingia-meningoseptica-in-cases-of-nosocomial-infections-and-patients-with-cystic-fibrosis
#5
É B de Carvalho Filho, F A L Marson, C E Levy
Rare nonfermenting Gram-negative bacilli, such as Chryseobacterium indologenes and Elizabethkingia meningoseptica, have clinical importance in nosocomial infections and cystic fibrosis (CF), and their identification is a challenge to microbiology laboratories. Thus, the objective of this study was to verify the performance of phenotypic and mass spectrometry (matrix-assisted desorption ionization-time of flight mass spectrometry, MALDI-TOF MS) methods to identify C. indologenes and E. meningoseptica. In this context, the results obtained with phenotypic methods-namely manual biochemical and automated VITEK 2 (bioMérieux, Marcy l'Etoile, France) and Phoenix tests (Becton Dickinson (BD), San Diego, CA, USA)-and by MALDI-TOF MS-namely MALDI-TOF VITEK MS (MALDI-MS; bioMérieux) and MALDI-TOF BioTyper (MALDI-BD; BD)-of 22 isolates (blood cultures of patients with nosocomial infection (n = 15) and from patients with CF (n = 7)), initially identified as C...
November 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/29062009/comparison-of-four-automated-microbiology-systems-with-16s-rrna-gene-sequencing-for-identification-of-chryseobacterium-and-elizabethkingia-species
#6
Jiun-Nong Lin, Chung-Hsu Lai, Chih-Hui Yang, Yi-Han Huang, Hsiu-Fang Lin, Hsi-Hsun Lin
Chryseobacterium and Elizabethkingia species have recently emerged as causative agents in life-threatening infections in humans. We aimed to evaluate the rates at which four common microbial identification systems identify Chryseobacterium and Elizabethkingia species in clinical microbiology laboratories. Based on the results of 16S rRNA gene sequencing, a total of 114 consecutive bacteremic isolates, including 36 (31.6%) C. indologenes, 35 (30.7%) E. anophelis, 22 (19.3%) C. gleum, 13 (11.4%) E. meningoseptica, and other species, were included in this study...
October 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29021156/safety-and-accuracy-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-to-identify-highly-pathogenic-organisms
#7
James T Rudrik, Marty K Soehnlen, Michael J Perry, Maureen Sullivan, Wanda Reiter-Kintz, Philip A Lee, Denise Pettit, Anthony Tran, Erin Swaney
Matrix-assisted laser desorption ionization -- time of flight mass spectrometry (MALDI-TOF MS) sample preparation methods including the direct, on-plate formic acid, and ethanol/formic acid tube extraction were evaluated for their ability to render highly pathogenic organisms non-viable and safe for handling in a Biosafety Level-2 laboratory. Of these, the tube extraction procedure was the most successful, with none of the tested strains surviving this sample preparation method. Tube extracts from several agents of bioterrorism and their near neighbors were analyzed in an eight laboratory study to examine the utility of the Bruker Biotyper and Vitek MS MALDI-TOF MS systems and their IVD, research use only, and Security-Relevant databases, as applicable, to accurately identify these agents...
October 11, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28949803/enterotoxin-production-and-antimicrobial-susceptibility-in-staphylococci-isolated-from-traditional-raw-milk-cheeses-in-serbia
#8
Snezana Bulajic, Svetlana Colovic, Dusan Misic, Jasna Djordjevic, Radoslava Savic-Radovanovic, Jelena Asanin, Tijana Ledina
This study was undertaken to determine the prevalence of coagulase positive staphylococci (CPS) by examining a total of 71 raw milk cheeses. Additionally, enterotoxigenicity, antimicrobial susceptibility and the presence of mecA and mecC genes in the staphylococcal isolates were investigated. The isolation and enumeration procedure of CPS followed the International Organization for Standardization (ISO) standard. The presumptive staphylococci were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using the VITEK MS system...
September 26, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/28929963/-comparison-of-phenotypic-methods-and-polymerase-chain-reaction-for-the-detection-of-carbapenemase-production-in-clinical-klebsiella-pneumoniae-isolates
#9
Yamaç Tekintaş, Feriha Çilli, Bayrı Eraç, Melike Yaşar, Sabire Şöhret Aydemir, Mine Hoşgör Limoncu
Being a member of the Enterobacteriaceae family, Klebsiella pneumoniae is an opportunistic pathogen that inhabits normal human microbiota and causes predominantly hospital-acquired infections. The emergence of K.pneumoniae isolates which are resistant particularly to the carbapenem group of antibiotics has led to an increase in hospitalization period, mortality and morbidity. Although different rates of resistance are observed between countries, regions and even healthcare facilities, there has been a rapid increase in the prevalence of carbapenem-resistant strains in the last 10 years...
July 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28924947/development-of-a-rapid-maldi-tof-ms-based-epidemiological-screening-method-using-mrsa-as-a-model-organism
#10
Åsa Lindgren, Nahid Karami, Roger Karlsson, Christina Åhrén, Martin Welker, Edward R B Moore, Liselott Svensson Stadler
In this study we present a method using whole cell MALDI-TOF MS and VITEK MS RUO/SARAMIS as a rapid epidemiological screening tool. MRSA was used as a model organism for setting up the screening strategy. A collection of well-characterised MRSA strains representing the 19 most common Pulsed-Field Gel Electrophoresis (PFGE)-types in the region of South-West Sweden for the past 20 years was analysed with MALDI-TOF MS. A total of 111 MRSA strains were used for creating 19 PFGE-specific Superspectra using VITEK MS RUO/SARAMIS...
September 18, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28911341/rapid-detection-of-staphylococcus-aureus-and-related-species-isolated-from-food-environment-cosmetics-a-medical-device-and-clinical-samples-using-the-vitek-ms-microbial-identification-system
#11
Irshad M Sulaiman, Pratik Banerjee, Ying-Hsin Hsieh, Nancy Miranda, Steven Simpson, Khalil Kerdahi
Staphylococcus spp. is considered as one of the most common human-pathogenic bacteria, causing illnesses ranging from nonthreatening skin infections to lethal diseases, including sepsis, pneumonia, bloodstream infections, and food poisoning. The emergence of methicillin-resistant Staphylococcus aureus strains has increased morbidity and mortality and resulted in a major healthcare burden worldwide. Single and multilocus sequence typing have been extensively used in the identification of Staphylococcus species...
September 15, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28899453/application-of-maldi-tof-ms-systems-in-the-rapid-identification-of-campylobacter-spp-of-public-health-importance
#12
Ying-Hsin Hsieh, Yun F Wang, Hercules Moura, Nancy Miranda, Steven Simpson, Ramnath Gowrishankar, John Barr, Khalil Kerdahi, Irshad M Sulaiman
Campylobacteriosis is an infectious gastrointestinal disease caused by Campylobacter spp.In most cases, it is either underdiagnosed or underreported due to poor diagnostics and limited databases. Several DNA-based molecular diagnostic techniques, including 16S ribosomal RNA (rRNA) sequence typing, have been widely used in the species identification of Campylobacter. Nevertheless, these assays are time-consuming and require a high quality of bacterial DNA. Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) MS is an emerging diagnostic technology that can provide the rapid identification of microorganisms by using their intact cells without extraction or purification...
September 12, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28888870/efficient-differentiation-of-corynebacterium-striatum-corynebacterium-amycolatum-and-corynebacterium-xerosis-clinical-isolates-by-multiplex-pcr-using-novel-species-specific-primers
#13
Carolina S Santos, Juliana N Ramos, Veronica V Vieira, Carina S Pinheiro, Roberto Meyer, Neuza M Alcantara-Neves, Rommel T Ramos, Artur Silva, Raphael Hirata, Liza Felicori, Carlos Ruiz de Alegría Puig, Jesús Navas, Vasco Azevedo, Ana L Mattos-Guaraldi, Luis G C Pacheco
A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226bp, 434bp and 106bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS.
November 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28817704/analysis-of-multidrug-resistant-group-b-streptococci-with-reduced-penicillin-susceptibility-forming-small-less-hemolytic-colonies
#14
Hirotsugu Banno, Kouji Kimura, Yosuke Tanaka, Tsuyoshi Sekizuka, Makoto Kuroda, Wanchun Jin, Jun-Ichi Wachino, Keiko Yamada, Keigo Shibayama, Yoshichika Arakawa
Group B streptococci (GBS; Streptococcus agalactiae) are the leading cause of neonatal invasive diseases and are also important pathogens for elderly adults. Until now, nearly all GBS with reduced penicillin susceptibility (PRGBS) have shown β-hemolytic activity and grow on sheep blood agar. However, we have previously reported three PRGBS clinical isolates harboring a CylK deletion that form small less hemolytic colonies. In this study, we examined the causes of small, less hemolytic colony formation in these clinical isolates...
2017: PloS One
https://www.readbyqxmd.com/read/28801661/statistical-characterization-of-therapeutic-protein-modifications
#15
Tsung-Heng Tsai, Zhiqi Hao, Qiuting Hong, Benjamin Moore, Cinzia Stella, Jeffrey H Zhang, Yan Chen, Michael Kim, Theo Koulis, Gregory A Ryslik, Erik Verschueren, Fred Jacobson, William E Haskins, Olga Vitek
Peptide mapping with liquid chromatography-tandem mass spectrometry (LC-MS/MS) is an important analytical method for characterization of post-translational and chemical modifications in therapeutic proteins. Despite its importance, there is currently no consensus on the statistical analysis of the resulting data. In this manuscript, we distinguish three statistical goals for therapeutic protein characterization: (1) estimation of site occupancy of modifications in one condition, (2) detection of differential site occupancy between conditions, and (3) estimation of combined site occupancy across multiple modification sites...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28747897/identification-of-emerging-human-mastitis-pathogens-by-maldi-tof-and-assessment-of-their-antibiotic-resistance-patterns
#16
María Marín, Rebeca Arroyo, Irene Espinosa-Martos, Leónides Fernández, Juan M Rodríguez
Lactational mastitis constitutes one of the main causes of undesired weaning, depriving the mother-infant pair from the benefits of breastfeeding; therefore, this condition should be considered a relevant public health issue. The role of specific microorganisms remains unclear since human milk cultures and antibiotic susceptibility testing (AST) are not routinely performed, despite the fact that this would be key to ensure an early and effective diagnosis and treatment. The objective of this study was to describe the culturable microbial diversity in 647 milk samples from breastfeeding women with clinical symptoms of mastitis by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF) VITEK MS technology and to analyze the antimicrobial susceptibility profiles of a collection of isolates from these samples by the VITEK 2 AST system...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28730548/orthopedic-infections-caused-by-obligatory-anaerobic-gram-negative-rods-report-of-two-cases
#17
Marta Kierzkowska, Piotr Pedzisz, Ireneusz Babiak, Jakub Janowicz, Mateusz Kulig, Anna Majewska, Anna Sawicka-Grzelak, Grazyna Mlynarczyk
Anaerobic bone and joint infections are uncommon, although the number of anaerobic infections is presumably underestimated because of difficulties with isolation and identification of obligate anaerobes. This study describes two cases of complicated Bacteroides fragilis peri-implant infection of the lumbar spine, infection of the hip and osteomyelitis. Bacteria were identified with the use of a mass spectrometer, VITEK MS system. Drug susceptibility was performed with the use of E-test. The EUCAST breakpoints were used for interpretation with B...
October 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28668254/corrigendum-to-maldi-tof-ms-is-more-accurate-than-vitek-ii-anc-card-and-api-rapid-id-32-a-system-for-the-identification-of-clostridium-species-anaerobe-40-2016-73-75
#18
Young Jin Kim, Si Hyun Kim, Hyun-Jung Park, Hae-Geun Park, Dongchul Park, Sae Am Song, Hee Joo Lee, Dongeun Yong, Jun Yong Choi, Joong-Ki Kook, Hye Ran Kim, Jeong Hwan Shin
No abstract text is available yet for this article.
June 28, 2017: Anaerobe
https://www.readbyqxmd.com/read/28658756/unusual-severe-extra-intestinal-manifestations-of-a-common-enteric-pathogen-aeromonas-spp
#19
Barnini Banerjee, Mridula Madiyal, Lingadakai Ramchandra, Chiranjay Mukhopadhyay, Rahul Garg, Kiran Chawla
INTRODUCTION: Aeromonas spp. are halophilic, Gram-negative bacilli. They are widely distributed in the soil and aquatic environment and have been associated with various extra-intestinal infections, such as skin and soft-tissue infections, meningitis, bacteraemia etc. The most common species associated with extra-intestinal infection is Aeromonas hydrophila. AIM: To get the overview of clinical presentations, underlying predisposing factors associated with the extra-intestinal infections caused by Aeromonas species and their antimicrobial susceptibility pattern...
May 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28656386/performance-of-microbial-identification-by-maldi-tof-ms-and-susceptibility-testing-by-vitek-2-from-positive-blood-cultures-after-minimal-incubation-on-solid-media
#20
S L Mitchell, K Alby
Bloodstream infections (BSIs) are a leading cause of patient morbidity and mortality. Rapid identification of organisms from BSIs is critical for initiating targeted antimicrobial therapy. Although many methods exist for rapid identification, they do not provide detailed or definitive susceptibility information. We assessed the utility of both the VITEK MS and Bruker Biotyper MALDI-TOF mass spectrometers to identify organisms from a positive blood culture bottle after only 4 h of growth on solid media compared to identification from overnight growth using the VITEK MS...
November 2017: European Journal of Clinical Microbiology & Infectious Diseases
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