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https://www.readbyqxmd.com/read/28100305/environment-directed-activation-the-escherichia-coli-flhdc-operon-by-transposons
#1
Zhongge Zhang, Chika Kukita, Mir Zafri Humayun, Milton H Saier
The flagellar system in E. coli K12 is expressed under the control of the flhDC-encoded master regulator, FlhDC. Transposition of insertion sequence (IS) elements to the upstream flhDC promoter region up-regulates transcription of this operon, resulting in more rapid motility. Wang and Wood (2011) provided evidence that insertion of IS5 into upstream activating sites occurs at higher rates in semisolid agar media in which swarming behavior is allowed as compared with liquid or solid media where swarming cannot occur...
January 17, 2017: Microbiology
https://www.readbyqxmd.com/read/28099173/surgical-approach-for-ulcerated-locally-advanced-breast-cancer-a-single-center-experience-a-retrospective-study
#2
Rita Laforgia, Clelia Punzo, Annunziata Panebianco, Annalisa Volpi, Marina Minafra, Maria Grazia Sederino
AIM: The aim of our retrospective study is to analyze surgical possibilities for the extended LABC in those cases not suitable for a neoadjuvant chemotherapy step and to consider various reconstruction techniques. MATERIAL OF STUDY: Between 2009 and 2015 we enrolled 11 patients, admitted to the Emergency Department, presenting ulcerated LABC that needed palliative surgical demolitive procedures because of bleeding and anemia and in which was necessary to use natural tissues transposition or synthetic substitutes for the reconstruction of the skin flaps...
January 12, 2017: Annali Italiani di Chirurgia
https://www.readbyqxmd.com/read/28098706/current-trends-in-ulnar-collateral-ligament-reconstruction-surgery-among-newly-trained-orthopaedic-surgeons
#3
Ryan M Degen, Christopher L Camp, Johnathan A Bernard, David M Dines, David W Altchek, Joshua S Dines
INTRODUCTION: Ulnar collateral ligament (UCL) reconstructions are being performed with an increasing annual incidence. The purpose of this study was to evaluate trends in UCL surgery among recently trained orthopaedic surgeons. METHODS: The American Board of Orthopaedic Surgeons (ABOS) database was used to identify all UCL reconstructions from 2004 to 2013. Procedures were identified by Current Procedural Terminology (CPT) codes and verified by International Classification of Disease, Ninth Revision (ICD-9) codes...
February 2017: Journal of the American Academy of Orthopaedic Surgeons
https://www.readbyqxmd.com/read/28096902/functional-characterization-of-the-active-mutator-like-transposable-element-muta1-from-the-mosquito-aedes-aegypti
#4
Kun Liu, Susan R Wessler
BACKGROUND: Mutator-like transposable elements (MULEs) are widespread with members in fungi, plants, and animals. Most of the research on the MULE superfamily has focused on plant MULEs where they were discovered and where some are extremely active and have significant impact on genome structure. The maize MuDR element has been widely used as a tool for both forward and reverse genetic studies because of its high transposition rate and preference for targeting genic regions. However, despite being widespread, only a few active MULEs have been identified, and only one, the rice Os3378, has demonstrated activity in a non-host organism...
2017: Mobile DNA
https://www.readbyqxmd.com/read/28096365/unlocking-tn3-family-transposase-activity-in-vitro-unveils-an-asymetric-pathway-for-transposome-assembly
#5
Emilien Nicolas, Cédric A Oger, Nathan Nguyen, Michaël Lambin, Amandine Draime, Sébastien C Leterme, Michael Chandler, Bernard F J Hallet
The Tn3 family is a widespread group of replicative transposons that are notorious for their contribution to the dissemination of antibiotic resistance and the emergence of multiresistant pathogens worldwide. The TnpA transposase of these elements catalyzes DNA breakage and rejoining reactions required for transposition. It also is responsible for target immunity, a phenomenon that prevents multiple insertions of the transposon into the same genomic region. However, the molecular mechanisms whereby TnpA acts in both processes remain unknown...
January 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28096347/human-transposon-insertion-profiling-analysis-visualization-and-identification-of-somatic-line-1-insertions-in-ovarian-cancer
#6
Zuojian Tang, Jared P Steranka, Sisi Ma, Mark Grivainis, Nemanja Rodić, Cheng Ran Lisa Huang, Ie-Ming Shih, Tian-Li Wang, Jef D Boeke, David Fenyö, Kathleen H Burns
Mammalian genomes are replete with interspersed repeats reflecting the activity of transposable elements. These mobile DNAs are self-propagating, and their continued transposition is a source of both heritable structural variation as well as somatic mutation in human genomes. Tailored approaches to map these sequences are useful to identify insertion alleles. Here, we describe in detail a strategy to amplify and sequence long interspersed element-1 (LINE-1, L1) retrotransposon insertions selectively in the human genome, transposon insertion profiling by next-generation sequencing (TIPseq)...
January 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28096324/totally-laparoscopic-liver-resection-for-colorectal-metastasis-located-in-segment-7-in-a-patient-with-situs-inversus-totalis
#7
Antonio Giuliani, Paolo Bianco, Germano Guerra, Aldo Rocca, Fulvio Calise
Situs inversus totalis (SIT) is a congenital condition consisting of a mirror image of transposition of the abdominal and thoracic organs occurring in about 1:5000 to 1:10 000 adults. We report on a 60-year-old male with a single colorectal liver metastasis in the Segment 7. The patients underwent a totally laparoscopic sub-segmentectomy. Intraoperative approach on a reverse posterior segment was difficult because of left-sided position of the liver. Postoperative course was uneventful and the patient was discharged after 5 days...
January 17, 2017: Journal of Surgical Case Reports
https://www.readbyqxmd.com/read/28095995/pelvic-floor-reconstruction-by-modified-rectus-abdominis-myoperitoneal-mram-flap-after-pelvic-exenterations
#8
D Cibula, M Zikan, D Fischerova, R Kocian, A Germanova, A Burgetova, L Dusek, Z Fartáková, M Schneiderová, K Nemejcová, J Slama
OBJECTIVE: To describe the technique and report experiences with pelvic floor reconstruction by modified rectus abdominis myoperitoneal (MRAM) flap after extensive pelvic procedures. METHODS: Surgical technique of MRAM harvest and transposition is carefully described. The patients in whom pelvic floor reconstruction with MRAM after either infralevator pelvic exenteration and/or extended lateral pelvic sidewall excision was carried out were enrolled into the study (MRAM group, n=16)...
January 14, 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/28095770/the-unique-genomic-landscape-surrounding-the-epsps-gene-in-glyphosate-resistant-amaranthus-palmeri-a-repetitive-path-to-resistance
#9
William T Molin, Alice A Wright, Amy Lawton-Rauh, Christopher A Saski
BACKGROUND: The expanding number and global distributions of herbicide resistant weedy species threaten food, fuel, fiber and bioproduct sustainability and agroecosystem longevity. Amongst the most competitive weeds, Amaranthus palmeri S. Wats has rapidly evolved resistance to glyphosate primarily through massive amplification and insertion of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene across the genome. Increased EPSPS gene copy numbers results in higher titers of the EPSPS enzyme, the target of glyphosate, and confers resistance to glyphosate treatment...
January 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28093020/what-causes-the-greater-perceived-similarity-of-consonant-transposed-nonwords
#10
Teresa Schubert, Sachiko Kinoshita, Dennis Norris
Nonwords created by transposing two non-adjacent orthographic consonants (CONDISER) have been reported to produce more priming for their baseword (CONSIDER), and to be classified as a nonword less readily than nonwords created by transposing two orthographic vowels (CINSODER). We investigate the origin of this difference and its relevance for theories of letter position coding. In the unprimed versions of the lexical decision and same-different tasks, a consonant-vowel difference was found in the transposition condition, not when those letters are substituted (Experiment 1)...
January 17, 2017: Quarterly Journal of Experimental Psychology: QJEP
https://www.readbyqxmd.com/read/28091712/two-cases-of-female-urethral-reconstruction-with-acellular-porcine-urinary-bladder-matrix
#11
Sana Ansari, Mickey Karram
INTRODUCTION AND HYPOTHESIS: Female urethral reconstruction via the traditional routes can be limiting for various reasons. Current literature on the use of acellular biologic grafts derived from viscera for female urethral reconstruction is limited. We present two cases of women with complete loss of their posterior urethra presenting for urethral reconstruction. METHODS: Two cases of urethral reconstruction using acellular porcine urinary bladder matrix (UBM), along with labial fat pad transposition and biologic pubovaginal sling are presented...
January 13, 2017: International Urogynecology Journal
https://www.readbyqxmd.com/read/28089807/ultrasonography-detects-ulnar-nerve-dislocation-despite-normal-electrophysiology-and-magnetic-resonance-imaging
#12
Jared M Pisapia, Zarina S Ali, Eric D Hudgins, Viviane Khoury, Gregory G Heuer, Eric L Zager
BACKGROUND: Dislocation of the ulnar nerve (UN) occurs in a subset of patients with ulnar neuropathy. Electrodiagnostic and magnetic resonance imaging (MRI) studies are performed to support the clinical diagnosis. We report the case of a patient with ulnar neuropathy with normal electrodiagnostic and MRI studies but with ultrasound (US) showing UN dislocation, which prompted successful treatment by UN submuscular transposition. CASE DESCRIPTION: A healthy 15 year-old female softball player presented with right medial elbow pain and paresthesias of the fourth and fifth digits...
January 12, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28088601/mid-term-outcomes-in-patients-with-congenitally-corrected-transposition-of-the-great-arteries-a-single-center-experience
#13
Luis E De León, Carlos M Mery, Raymond A Verm, Daniel Trujillo-Díaz, Ankita Patro, Francisco A Guzmán-Pruneda, Iki Adachi, Jeffrey S Heinle, Lauren C Kane, E Dean McKenzie, Charles D Fraser
INTRO: The optimal management of patients with congenitally-corrected transposition of the great arteries (ccTGA) is unclear. The goal of this study was to compare the outcomes in patients with ccTGA undergoing different management strategies. METHODS: Patients with ccTGA believed suitable for biventricular circulation treated in 1995-2016 were included. The cohort was divided into four groups: systemic right ventricle (RV) (patients without surgical intervention or with a classic repair), anatomic repair, Fontan palliation, and patients only receiving a pulmonary artery band (PAB) or a shunt...
January 11, 2017: Journal of the American College of Surgeons
https://www.readbyqxmd.com/read/28077506/clinical-guidelines-for-the-management-of-patients-with-transposition-of-the-great-arteries-with-intact-ventricular-septum
#14
George E Sarris, Christian Balmer, Pipina Bonou, Juan V Comas, Eduardo da Cruz, Luca Di Chiara, Roberto M Di Donato, José Fragata, Tuula Eero Jokinen, George Kirvassilis, Irene Lytrivi, Milan Milojevic, Gurleen Sharland, Matthias Siepe, Joerg Stein, Emanuela Valsangiacomo Büchel, Pascal R Vouhé
No abstract text is available yet for this article.
January 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28077501/guidelines-for-transposition-surgery-and-why-we-need-them
#15
EDITORIAL
Tom R Karl
No abstract text is available yet for this article.
January 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28077189/cardiac-haemangioma-associated-with-a-duct-dependent-congenital-heart-disease-in-a-newborn-infant
#16
Stefan A Djordjevic, Sofija Glumac, Jasna Kalanj
Cardiac haemangiomas are exceedingly rare; however, they can cause significant haemodynamic impairment and disturbances in heart rhythm. Rarely, cardiac tumours may also coexist with congenital heart lesions. We present an extremely unusual case of a cardiac haemangioma in the setting of complex transposition of the great arteries that caused functional tricuspid atresia. To our knowledge, this is the first such case described in the literature.
January 12, 2017: Cardiology in the Young
https://www.readbyqxmd.com/read/28070723/anorectal-malformations-definitive-surgery-during-adulthood
#17
M P J Lopez, V I Encila, S G Alamo, H J Monroy Iii, M F T Roxas
BACKGROUND: Anorectal malformations (ARMs) are rarely seen in adults, since majority of cases are corrected in infancy or childhood. The aim of this study was to describe the profile of patients who underwent definitive surgery to correct their ARM in adulthood, and to discuss the outcomes of surgery (morbidity, mortality, and function). METHODS: This retrospective study included patients 18 years old and above, managed surgically by the Division of Colorectal Surgery at the Philippine General Hospital, University of the Philippines, Manila, from January 1, 2004, to December 31, 2012...
January 9, 2017: Techniques in Coloproctology
https://www.readbyqxmd.com/read/28068842/tibialis-anterior-tendon-reconstruction-using-augmented-half-thickness-tendon-segment-transposition
#18
Christopher W Reb, James F Stenson, Joseph N Daniel
: Tibialis anterior tendon rupture causes substantial morbidity. The present study describes the outcomes of augmented, half-thickness tibialis anterior tendon segment transposition, a novel reconstruction technique. This was an institutional review board-approved retrospective review of 3 patients with surgically treated attritional distal rupture with 1-year follow-up. The postdebridement tendon defect prohibited primary repair and was managed by distal transposition of a half-thickness healthy segment...
January 1, 2017: Foot & Ankle Specialist
https://www.readbyqxmd.com/read/28068316/scalable-whole-genome-single-cell-library-preparation-without-preamplification
#19
Hans Zahn, Adi Steif, Emma Laks, Peter Eirew, Michael VanInsberghe, Sohrab P Shah, Samuel Aparicio, Carl L Hansen
Single-cell genomics is critical for understanding cellular heterogeneity in cancer, but existing library preparation methods are expensive, require sample preamplification and introduce coverage bias. Here we describe direct library preparation (DLP), a robust, scalable, and high-fidelity method that uses nanoliter-volume transposition reactions for single-cell whole-genome library preparation without preamplification. We examined 782 cells from cell lines and triple-negative breast xenograft tumors. Low-depth sequencing, compared with existing methods, revealed greater coverage uniformity and more reliable detection of copy-number alterations...
January 9, 2017: Nature Methods
https://www.readbyqxmd.com/read/28065912/the-use-of-contrast-echo-to-detect-shunts-at-atrial-level-in-a-mustard-or-senning-repair-for-transposition-of-the-great-arteries-you-can-teach-an-old-dog-new-tricks
#20
EDITORIAL
Folkert Meijboom, Berto Bouma
No abstract text is available yet for this article.
January 8, 2017: European Heart Journal Cardiovascular Imaging
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