keyword
MENU ▼
Read by QxMD icon Read
search

Screening multiples

keyword
https://www.readbyqxmd.com/read/29228468/characterization-of-forsythoside-a-metabolites-in-rats-by-a-combination-of-uhplc-ltq-orbitrap-mass-spectrometer-with-multiple-data-processing-techniques
#1
Fei Wang, Guang-Shang Cao, Yun Li, Lu-Lu Xu, Zhi-Bin Wang, Ying Liu, Jian-Qiu Lu, Jia-Yu Zhang
Forsythoside A (FTA), the main active constituent isolated from Fructus Forsythiae, has various biological functions including anti-oxidant, anti-viral and anti-microbial activities. However, while researches on FTA have been mainly focused on the treatment of diseases on material basis, FTA metabolites in vivo have not been comprehensively evaluated. Here, a rapid and sensitive method using UHPLC-LTQ-Orbitrap mass spectrometer with multiple data processing techniques including high-resolution extracted ion chromatograms (HREICs), multiple mass defect filters (MMDFs) and diagnostic product ions (DPIs) was developed for screening and identification of FTA metabolites in rats...
December 11, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29228274/screening-the-toxcast-phase-1-chemical-library-for-inhibition-of-deiodinase-type-1-activity
#2
Michael W Hornung, Joseph J Korte, Jennifer H Olker, Jeffrey S Denny, Carsten Knutsen, Phillip C Hartig, Mary C Cardon, Sigmund J Degitz
Thyroid hormone (TH) homeostasis is dependent upon coordination of multiple key events including iodide uptake, hormone synthesis, metabolism and elimination, to maintain proper TH signaling. Deiodinase enzymes catalyze iodide release from THs to interconvert THs between active and inactive forms, and are integral to hormone metabolism. The activity of deiodinases has been identified as an important endpoint to include in the context of screening chemicals for thyroid hormone disruption. To begin to address the potential for chemicals to inhibit these enzymes an adenovirus expression system was used to produce human deiodinase type 1 (DIO1) enzyme, established robust assay parameters for non-radioactive determination of iodide release by the Sandell-Kolthoff method, and employed a 96-well plate format for screening chemical libraries...
December 7, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29228130/acute-retroviral-syndrome-is-associated-with-high-viral-burden-cd4-depletion-and-immune-activation-in-systemic-and-tissue-compartments
#3
Trevor A Crowell, Donn J Colby, Suteeraporn Pinyakorn, James L K Fletcher, Eugène Kroon, Alexandra Schuetz, Shelly J Krebs, Bonnie M Slike, Louise Leyre, Nicolas Chomont, Linda L Jagodzinski, Irini Sereti, Netanya S Utay, Robin Dewar, Rungsun Rerknimitr, Nitiya Chomchey, Rapee Trichavaroj, Victor G Valcour, Serena Spudich, Nelson L Michael, Merlin L Robb, Nittaya Phanuphak, Jintanat Ananworanich
Background: Many individuals with acute HIV infection (AHI) experience acute retroviral syndrome (ARS), which is associated with adverse long-term clinical outcomes. We characterized clinical, virologic, and immunologic features of ARS and described the impact of early antiretroviral therapy (ART) initiation. Methods: Participants presenting for voluntary HIV testing were enrolled during AHI in Bangkok, Thailand. ARS was defined by ≥3 qualifying signs/symptoms...
December 7, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29227997/normalized-emphysema-scores-on-low-dose-ct-validation-as-an-imaging-biomarker-for-mortality
#4
Leticia Gallardo-Estrella, Esther Pompe, Pim A de Jong, Colin Jacobs, Eva M van Rikxoort, Mathias Prokop, Clara I Sánchez, Bram van Ginneken
The purpose of this study is to develop a computed tomography (CT) biomarker of emphysema that is robust across reconstruction settings, and evaluate its ability to predict mortality in patients at high risk for lung cancer. Data included baseline CT scans acquired between August 2002 and April 2004 from 1737 deceased subjects and 5740 surviving controls taken from the National Lung Screening Trial. Emphysema scores were computed in the original scans (origES) and after applying resampling, normalization and bullae analysis (normES)...
2017: PloS One
https://www.readbyqxmd.com/read/29227800/assessment-of-the-neuropsychiatric-comorbidities-in-chinese-children-with-epilepsy-using-the-mini-kid-tool
#5
Taoli Li, Hao Zhou, Yijie Li, Chunpei Li, Yunjian Zhang, Yuanfeng Zhou, Yi Wang
This study aimed to assess neuropsychiatric comorbidities and analyze risk factors in Chinese children with epilepsy. Children with epilepsy aged between 6 and 16 years from the Children's Hospital of Fudan University were included. Children with asthma and typically developing children were matched for age and gender, and served as control groups. Neuropsychiatric disorders were assessed by interviewing the parents or guardians using the Mini International Neuropsychiatric Interview for children (MINI-KID) (parent version)...
November 24, 2017: Epilepsy Research
https://www.readbyqxmd.com/read/29227799/cognitive-function-after-status-epilepticus-versus-after-multiple-generalized-tonic-clonic-seizures
#6
Kjersti N Power, Arne Gramstad, Nils Erik Gilhus, Karl Ove Hufthammer, Bernt A Engelsen
OBJECTIVES: Status epilepticus (SE) is considered a risk for cognitive impairment. Studies have indicated that SE cause more cognitive decline than multiple lifetime generalized tonic clonic (GTC) seizures. The aim of the study was to investigate whether patients suffering from SE or from multiple lifetime GTC seizures have cognitive dysfunction, and if the disabilities differ between these groups. MATERIALS AND METHODS: Patients suffering from SE were evaluated shortly after the clinical post-ictal phase and again after one year...
November 28, 2017: Epilepsy Research
https://www.readbyqxmd.com/read/29227495/fluorescence-activated-droplet-sorting-of-lipolytic-microorganisms-using-a-compact-optical-system
#7
Yuxin Qiao, Xiaoyan Zhao, Jun Zhu, Ran Tu, Libing Dong, Li Wang, Zhiyang Dong, Qinhong Wang, Wenbin Du
Lipases are ubiquitous enzymes of great physiological significance that have been used extensively in multiple industries. Environmental microorganisms are a major source for the discovery of novel lipases with high catalytic efficiency and selectivity. However, current plate-based screening of lipase-producing strains is time consuming, labour intensive and inefficient. In this study, we developed an ultra-high throughput screening pipeline for lipase-producing strains based on fluorescence-activated droplet sorting (FADS) using a compact optical system that could be easily set up in an alignment-free manner...
December 11, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29226957/pilot-multi-centre-randomised-trial-of-the-impact-of-pre-operative-focused-cardiac-ultrasound-on-mortality-and-morbidity-in-patients-having-surgery-for-femoral-neck-fractures-echonof-2-pilot
#8
D J Canty, J Heiberg, Y Yang, A G Royse, S Margale, N Nanjappa, D Scott, A Maier, D I Sessler, A Chuan, A Palmer, A Bucknill, C French, C F Royse
Hip fracture surgery is common, usually occurs in elderly patients who have multiple comorbidities, and is associated with high morbidity and mortality. Pre-operative focused cardiac ultrasound can alter diagnosis and management, but its impact on outcome remains uncertain. This pilot study assessed feasibility and group separation for a proposed large randomised clinical trial of the impact of pre-operative focused cardiac ultrasound on patient outcome after hip fracture surgery. Adult patients requiring hip fracture surgery in four teaching hospitals in Australia were randomly allocated to receive focused cardiac ultrasound before surgery or not...
December 11, 2017: Anaesthesia
https://www.readbyqxmd.com/read/29226926/qsar-modeling-of-cumulative-environmental-end-points-for-the-prioritization-of-hazardous-chemicals
#9
Paola Gramatica, Ester Papa, Alessandro Sangion
The hazard of chemicals in the environment is inherently related to the molecular structure and derives simultaneously from various chemical properties/activities/reactivities. Models based on Quantitative Structure Activity Relationships (QSARs) are useful to screen, rank and prioritize chemicals that may have an adverse impact on humans and the environment. This paper reviews a selection of QSAR models (based on theoretical molecular descriptors) developed for cumulative multivariate endpoints, which were derived by mathematical combination of multiple effects and properties...
December 11, 2017: Environmental Science. Processes & Impacts
https://www.readbyqxmd.com/read/29226789/the-emotional-distress-of-asylum-seekers-in-israel-and-the-characteristics-of-those-seeking-psychiatric-versus-medical-help
#10
Naama Kiat, Rafael Youngmann, Ido Lurie
Israel has become a destination for asylum seekers. Asylum seekers often experience emotional distress, but have limited access to health services and rarely use psychiatric services. This study sought to understand and characterize the use of psychiatric versus medical services by asylum seekers in Israel. We compared the emotional distress, stressful life events and previous treatment consultations of 21 psychiatric service users (PSU) and 55 medical service users (MSU) at the Open Clinic of Physicians for Human Rights in Tel-Aviv...
October 2017: Transcultural Psychiatry
https://www.readbyqxmd.com/read/29225914/status-of-hypertension-screening-in-the-korea-national-general-health-screening-program-a-questionnaire-survey-on-210-screening-centers-in-two-metropolitan-areas
#11
Seung Won Lee, Hae-Young Lee, Sang Hyun Ihm, Sung Ha Park, Tae Hyun Kim, Hyeon Chang Kim
Background: The purpose of this survey was to evaluate the performance of hypertension screening in medical institutions conducting the national general health screening program of the Republic of Korea. Methods: We contacted 700 medical institutions of Seoul and Incheon areas which performed the national general health screening program in 2016, and 210 of them completed telephone survey. The questions asked in the survey include equipment, environment, personnel and quality control procedures for blood pressure (BP) measurement, and interpretation of the measurements...
2017: Clinical Hypertension
https://www.readbyqxmd.com/read/29225720/new-frontiers-in-molecular-and-imaging-research-on-evaluation-and-diagnosis-of-bladder-outlet-obstruction-in-women
#12
Laura Martinez, Rose Khavari
Purpose: Diagnosis of bladder outlet obstruction (BOO) often presents a challenge in female patients. Traditional diagnostic elements such as symptom history and urodynamic data are rarely clear cut in women. Therefore, we sought to review the current literature on diagnosis of female BOO, focusing on new frontiers in the realm of molecular markers and imaging modalities. Recent Findings: In addition to fluoroscopy in the setting of videourodynamics, ultrasound and MRI can augment the diagnosis and aid in therapeutic planning in certain etiologies of female BOO...
December 2017: Current Bladder Dysfunction Reports
https://www.readbyqxmd.com/read/29225663/high-throughput-detection-of-antioxidants-in-mulberry-fruit-using-correlations-between-high-resolution-mass-and-activity-profiles-of-chromatographic-fractions
#13
Ye Ji Park, Si Hyun Seong, Min Sun Kim, Sang Wan Seo, Mee Ree Kim, Hyun Sik Kim
Background: Plant extracts contain a huge variety of pharmacologically active substances. Conventionally, various chromatographic methods must be applied several times to purify functional compounds to measure their functional activity. However, conventional purification methods are time-consuming and expensive due to the laborious purification process. Recently, a high-throughput discovery method that replaces such time-consuming purification processes was introduced; this method uses 15 T ultra-high-resolution Fourier transform ion cyclotron resonance mass spectrometry (15 T FT-ICR MS) and a high-throughput screening method...
2017: Plant Methods
https://www.readbyqxmd.com/read/29224880/the-effect-of-different-treatments-for-early-lactation-hyperketonemia-on-liver-triglycerides-glycogen-and-expression-of-key-metabolic-enzymes-in-dairy-cattle
#14
S Mann, F A Leal Yepes, J J Wakshlag, E Behling-Kelly, J A A McArt
Despite the widespread use of treatments for postpartum hyperketonemia in dairy cows, there is currently a lack of evidence comparing their effects on both the resolution of hyperketonemia and the potential effects on the liver of affected animals. The objective of our work was to investigate the effect of commonly used hyperketonemia treatments on hepatic triglyceride and glycogen content as well as on the mRNA and protein abundance of key enzymes involved in gluconeogenesis, ketogenesis, and lipid metabolism...
December 7, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29224780/a-living-biobank-of-breast-cancer-organoids-captures-disease-heterogeneity
#15
Norman Sachs, Joep de Ligt, Oded Kopper, Ewa Gogola, Gergana Bounova, Fleur Weeber, Anjali Vanita Balgobind, Karin Wind, Ana Gracanin, Harry Begthel, Jeroen Korving, Ruben van Boxtel, Alexandra Alves Duarte, Daphne Lelieveld, Arne van Hoeck, Robert Frans Ernst, Francis Blokzijl, Isaac Johannes Nijman, Marlous Hoogstraat, Marieke van de Ven, David Anthony Egan, Vittoria Zinzalla, Jurgen Moll, Sylvia Fernandez Boj, Emile Eugene Voest, Lodewyk Wessels, Paul Joannes van Diest, Sven Rottenberg, Robert Gerhardus Jacob Vries, Edwin Cuppen, Hans Clevers
Breast cancer (BC) comprises multiple distinct subtypes that differ genetically, pathologically, and clinically. Here, we describe a robust protocol for long-term culturing of human mammary epithelial organoids. Using this protocol, >100 primary and metastatic BC organoid lines were generated, broadly recapitulating the diversity of the disease. BC organoid morphologies typically matched the histopathology, hormone receptor status, and HER2 status of the original tumor. DNA copy number variations as well as sequence changes were consistent within tumor-organoid pairs and largely retained even after extended passaging...
December 5, 2017: Cell
https://www.readbyqxmd.com/read/29224156/multiplexed-and-sensitive-dna-methylation-testing-using-methylation-sensitive-restriction-enzymes-msre-qpcr
#16
Gabriel Beikircher, Walter Pulverer, Manuela Hofner, Christa Noehammer, Andreas Weinhaeusel
DNA methylation is a chemically stable key-player in epigenetics. In the vertebrate genome the 5-methyl cytosine (5mC) has been found almost exclusively in the CpG dinucleotide context. CpG dinucleotides are enriched in CpG islands very frequently located within or close to gene promoters. Analyses of DNA methylation changes in human diagnostics have been conducted classically using methylation-sensitive restriction enzymes (MSRE). Since the discovery of bisulfite conversion-based sequencing and PCR assays, MSRE-based PCR assays have been less frequently used, although especially in the field of cancer epigenetics MSRE-based genome-wide discovery and targeted screening applications have been and are still performed successfully...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224103/sex-differences-and-role-of-estradiol-in-hypoglycemia-associated-counter-regulation
#17
Karen P Briski, Hussain N Alhamami, Ayed Alshamrani, Santosh K Mandal, Manita Shakya, Mostafa H H Ibrahim
Vital nerve cell functions, including maintenance of transmembrane voltage and information transfer, occur at high energy expense. Inadequate provision of the obligate metabolic fuel glucose exposes neurons to risk of dysfunction or injury. Clinical hypoglycemia rarely occurs in nondiabetic individuals but is an unfortunate regular occurrence in patients with type 1 or advanced insulin-treated type 2 diabetes mellitus. Requisite strict glycemic control, involving treatment with insulin, sulfonylureas, or glinides, can cause frequent episodes of iatrogenic hypoglycemia due to defective counter-regulation, including reduced glycemic thresholds and diminished magnitude of motor responses...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29224008/mirnas-mediate-gdnf-induced-proliferation-and-migration-of-glioma-cells
#18
Bao-Le Zhang, Fu-Lu Dong, Ting-Wen Guo, Xiao-He Gu, Lin-Yan Huang, Dian-Shuai Gao
BACKGROUND/AIMS: Glial cell line-derived neurotrophic factor (GDNF) is an important factor promoting invasive glioma growth. This study was performed to reveal a unique mechanism of glioma cell proliferation and migration. METHODS: Human U251 glioma cells were used to screen the optimal GDNF concentration and treatment time to stimulate proliferation and migration. MicroRNA (MiRNA) expression profiles were detected by microarray and confirmed by real-time polymerase chain reaction (PCR)...
December 8, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29223727/urodynamic-characteristics-might-be-variable-in-bladder-pain-syndrome-interstitial-cystitis-patients-with-different-non-bladder-co-morbid-conditions
#19
Wei-Ming Cheng, Yu-Hua Fan, Alex T L Lin
BACKGROUND: The aim of the study was to identify the impact of non-bladder co-morbid conditions on the urodynamic characteristics of patients with bladder pain syndrome/interstitial cystitis. METHODS: Patients with bladder pain syndrome/interstitial cystitis completed the screening questionnaires for chronic fatigue syndrome, irritable bowel syndrome, fibromyalgia, temporo-mandibular disorders, multiple chemical sensitivities, tension/migraine headache, and localized myofascial pain disorder...
December 6, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/29223326/effects-of-perceived-discrimination-and-trust-on-breast-cancer-screening-among-korean-american-women
#20
Hye Chong Hong, Carol Estwing Ferrans, Chang Park, Hyeonkyeong Lee, Lauretta Quinn, Eileen G Collins
OBJECTIVE: Korean American (KA) women continue to have lower breast cancer screening rates than other racial groups. Perceived discrimination and trust have been associated with breast cancer screening adherence, but little is known about the associations in KA women. METHODS: Surveys were completed by 196 KA women in the Chicago metropolitan area. Multiple and Firth logistic regression analyses were performed to identify factors (perceived discrimination, trust, acculturation, cultural beliefs, health care access) influencing breast cancer screening adherence (mammogram)...
December 6, 2017: Women's Health Issues: Official Publication of the Jacobs Institute of Women's Health
keyword
keyword
113637
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"