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https://www.readbyqxmd.com/read/28798237/decorin-evoked-paternally-expressed-gene-3-peg3-is-an-upstream-regulator-of-the-transcription-factor-eb-tfeb-in-endothelial-cell-autophagy
#1
Thomas Neill, Catherine Sharpe, Rick T Owens, Renato V Iozzo
Macroautophagy is a fundamental and evolutionarily conserved catabolic process that eradicates damaged and aging macromolecules and organelles in eukaryotic cells. Decorin, an archetypical small leucine-rich proteoglycan, initiates a protracted autophagic program downstream of VEGF receptor 2 (VEGFR2) signaling that requires paternally expressed gene 3 (PEG3). We have discovered that PEG3 is an upstream transcriptional regulator of transcription factor EB (TFEB), a master transcription factor of lysosomal biogenesis, for decorin-evoked endothelial cell autophagy...
September 29, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28599447/recombinant-fibromodulin-and-decorin-effects-on-nf-%C3%AE%C2%BAb-and-tgf%C3%AE-1-in-the-4t1-breast-cancer-cell-line
#2
Ladan Dawoody Nejad, Alireza Biglari, Tiziana Annese, Domenico Ribatti
Constitutive activation of nuclear factor-κB (NF-κB) stimulates cell proliferation and metastasis, and inhibits apoptosis in breast cancer. Transforming growth factor-β (TGF-β) signaling pathway is deregulated in breast cancer progression and metastasis. The aim of the present study was to investigate the inhibitory effects of the two small leucine rich proteoglycans fibromodulin (Fmod) and decorin (Dcn), overexpressed using adenovirus gene transfer, on NF-κB-activity and TGF-β1-expression in the highly metastatic 4T1 breast cancer cell line...
June 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454729/activation-of-parathyroid-hormone-2-receptor-induces-decorin-expression-and%C3%A2-promotes-wound-repair
#3
Emi Sato, Ling-Juan Zhang, Robert A Dorschner, Christopher A Adase, Biswa P Choudhury, Richard L Gallo
In this study, we report that TIP39, a parathyroid hormone ligand family member that was recently identified to be expressed in the skin, can induce decorin expression and enhance wound repair. Topical treatment of mice with TIP39 accelerated wound repair, whereas TIP39-deficient mice had delayed repair that was associated with formation of abnormal collagen bundles. To study the potential mechanism responsible for the action of TIP39 in the dermis, fibroblasts were cultured in three-dimensional collagen gels, a process that results in enhanced decorin expression unless activated to differentiate to adipocytes, whereupon these cells reduce expression of several proteoglycans, including decorin...
August 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/26791991/histological-and-biochemical-characteristics-of-the-rabbit-anterior-cruciate-ligament-in-comparison-to-potential-autografts
#4
Mariann Hoyer, Carola Meier, Benjamin Kohl, Anke Lohan, Maria Kokozidou, Gundula Schulze-Tanzil
Tissue engineering of an anterior cruciate ligament (ACL) implant with ACL cells requires detailed analysis of the tissue characteristics that should be mimicked. Therefore, we studied the histological and biochemical properties of rabbit derived ACLs in comparison to other knee-associated tendons that are used as autografts in men. Rabbit derived ACLs and Musculus (M.) semimembranosus, M. semitendinosus tendons and patellar ligaments were explanted from adult New Zealand white rabbits and analyzed histologically for tissue organization (e...
August 2016: Histology and Histopathology
https://www.readbyqxmd.com/read/26703441/soluble-biglycan-a-potential-mediator-of-cartilage-degradation-in-osteoarthritis
#5
Goncalo Barreto, Antti Soininen, Pekka Ylinen, Jerker Sandelin, Yrjö T Konttinen, Dan C Nordström, Kari K Eklund
BACKGROUND: Soluble biglycan (sBGN) and soluble decorin (sDCN), are two closely related essential components of extracellular matrix which both have been shown to possess proinflammatory properties. We studied whether sBGN or sDCN were present in synovial fluid (SF) of osteoarthritis (OA) or rheumatoid arthritis (RA) patients and studied sBGN or sDCN potential role in the degradation of OA cartilage. METHODS: SF obtained from meniscus tear, OA, and RA patients were analysed for sBGN and sDCN using enzyme-linked immunosorbent assays...
2015: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/26511275/sall1-in-renal-stromal-progenitors-non-cell-autonomously-restricts-the-excessive-expansion-of-nephron-progenitors
#6
Tomoko Ohmori, Shunsuke Tanigawa, Yusuke Kaku, Sayoko Fujimura, Ryuichi Nishinakamura
The mammalian kidney develops from reciprocal interactions between the metanephric mesenchyme and ureteric bud, the former of which contains nephron progenitors. The third lineage, the stroma, fills up the interstitial space and is derived from distinct progenitors that express the transcription factor Foxd1. We showed previously that deletion of the nuclear factor Sall1 in nephron progenitors leads to their depletion in mice. However, Sall1 is expressed not only in nephron progenitors but also in stromal progenitors...
2015: Scientific Reports
https://www.readbyqxmd.com/read/25695518/structural-mechanisms-underlying-sequence-dependent-variations-in-gag-affinities-of-decorin-binding-protein-a-a-borrelia-burgdorferi-adhesin
#7
Ashli M Morgan, Xu Wang
Decorin-binding protein A (DBPA) is an important surface adhesin of the bacterium Borrelia burgdorferi, the causative agent of Lyme disease. DBPA facilitates the bacteria's colonization of human tissue by adhering to glycosaminoglycan (GAG), a sulfated polysaccharide. Interestingly, DBPA sequence variation among different strains of Borrelia spirochetes is high, resulting in significant differences in their GAG affinities. However, the structural mechanisms contributing to these differences are unknown. We determined the solution structures of DBPAs from strain N40 of B...
May 1, 2015: Biochemical Journal
https://www.readbyqxmd.com/read/25012297/spatial-and-temporal-changes-of-decorin-type-i-collagen-and-fibronectin-expression-in-normal-and-clone-bovine-placenta
#8
M Guillomot, E Campion, A Prézelin, O Sandra, I Hue, D Le Bourhis, C Richard, F H Biase, C Rabel, R Wallace, H Lewin, J-P Renard, H Jammes
INTRODUCTION: Alteration of expression of various genes including extracellular matrix components, have been suggested to play major role in the placental pathologies after somatic cloning in mammals. The objectives of the present study were to analyze pattern of expression (mRNA and protein) of the small leucine-rich proteoglycan, Decorin in association with Type I Collagen and Fibronectin in bovine placental tissues from normal and clone pregnancies. METHODS: Genotyping and allelic expression of Decorin were determined by Sanger sequencing...
September 2014: Placenta
https://www.readbyqxmd.com/read/24966230/fibromodulin-and-biglycan-modulate-periodontium-through-tgf%C3%AE-bmp-signaling
#9
L Wang, B L Foster, V Kram, F H Nociti, P M Zerfas, A B Tran, M F Young, M J Somerman
A full understanding of the key regulators controlling periodontal development and homeostasis is necessary for the design of improved periodontal regenerative therapies. Small leucine-rich proteoglycans (SLRPs) are extracellular matrix molecules suggested to regulate collagen organization and cell signaling. Mice with double-deficiency of 2 SLRPs, fibromodulin and biglycan (dKO), acquire skeletal abnormalities, but their roles in regulating the periodontium remain undefined and were the focus of our studies...
August 2014: Journal of Dental Research
https://www.readbyqxmd.com/read/24842928/identification-of-lysine-residues-in-the-borrelia-burgdorferi-dbpa-adhesin-required-for-murine-infection
#10
Danielle E Fortune, Yi-Pin Lin, Ranjit K Deka, Ashley M Groshong, Brendan P Moore, Kayla E Hagman, John M Leong, Diana R Tomchick, Jon S Blevins
Decorin-binding protein A (DbpA) of Borrelia burgdorferi mediates bacterial adhesion to heparin and dermatan sulfate associated with decorin. Lysines K82, K163, and K170 of DbpA are known to be important for in vitro interaction with decorin, and the DbpA structure, initially solved by nuclear magnetic resonance (NMR) spectroscopy, suggests these lysine residues colocalize in a pocket near the C terminus of the protein. In the current study, we solved the structure of DbpA from B. burgdorferi strain 297 using X-ray crystallography and confirmed the existing NMR structural data...
August 2014: Infection and Immunity
https://www.readbyqxmd.com/read/24834008/regulation-of-tenomodulin-expression-via-wnt-%C3%AE-catenin-signaling-in-equine-bone-marrow-derived-mesenchymal-stem-cells
#11
Shihori Miyabara, Yohei Yuda, Yoshinori Kasashima, Atsutoshi Kuwano, Katsuhiko Arai
Tenomodulin has been recognized as a biomarker for tendon differentiation, and its gene expression is regulated by several transcription factors including Scleraxis and Mohawk. In this study, we found a novel regulatory mechanism of tenomodulin expression. Equine bone marrow-derived mesenchymal stem cells (BMSCs) in monolayer culture showed a low mRNA level of tenomodulin in comparison with the level in the tendon. When cultured in collagen gel containing a glycogen synthase kinase-3 (GSK-3) inhibitor (BIO), expression of tenomodulin in BMSCs increased up to the level in the tendon...
2014: Journal of Equine Science
https://www.readbyqxmd.com/read/24448801/calcium-efflux-activity-of-plasma-membrane-ca2-atpase-4-pmca4-mediates-cell-cycle-progression-in-vascular-smooth-muscle-cells
#12
Talat Afroze, Ge Yang, Amir Khoshbin, Mansoor Tanwir, Taha Tabish, Abdul Momen, Mansoor Husain
We explored the role played by plasma membrane calcium ATPase-4 (PMCA4) and its alternative splice variants in the cell cycle of vascular smooth muscle cells (VSMC). A novel variant (PMCA4e) was discovered. Quantitative real-time-PCR-quantified PMCA4 splice variant proportions differed in specific organs. The PMCA4a:4b ratio in uninjured carotid arteries (∼1:1) was significantly reduced by wire denudation injury (to ∼1:3) by modulation of alternative splicing, as confirmed by novel antibodies against PMCA4a/e and PMCA4b...
March 7, 2014: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/24361760/generation-of-a-neurodegenerative-disease-mouse-model-using-lentiviral-vectors-carrying-an-enhanced-synapsin-i-promoter
#13
Yasunori Matsuzaki, Miho Oue, Hirokazu Hirai
BACKGROUND: Certain inherited progressive neurodegenerative disorders, such as spinocerebellar ataxia (SCA), affect neurons in large areas of the central nervous system (CNS). The selective expression of disease-causing and therapeutic genes in susceptible regions and cell types is critical for the generation of animal models and development of gene therapies for these diseases. Previous studies have demonstrated the advantages of the short synapsin I (SynI) promoter (0.5 kb) as a neuron-specific promoter for robust transgene expression...
February 15, 2014: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/24284212/foxd1-promotes-nephron-progenitor-differentiation-by-repressing-decorin-in-the-embryonic-kidney
#14
Jennifer L Fetting, Justin A Guay, Michele J Karolak, Renato V Iozzo, Derek C Adams, David E Maridas, Aaron C Brown, Leif Oxburgh
Forkhead transcription factors are essential for diverse processes in early embryonic development and organogenesis. Foxd1 is required during kidney development and its inactivation results in failure of nephron progenitor cell differentiation. Foxd1 is expressed in interstitial cells adjacent to nephron progenitor cells, suggesting an essential role for the progenitor cell niche in nephrogenesis. To better understand how cortical interstitial cells in general, and FOXD1 in particular, influence the progenitor cell niche, we examined the differentiation states of two progenitor cell subtypes in Foxd1(-/-) tissue...
January 2014: Development
https://www.readbyqxmd.com/read/24152495/molecular-and-phenotypic-expression-of-decorin-as-modulator-of-angiogenesis-in-human-potentially-malignant-oral-lesions-and-oral-squamous-cell-carcinomas
#15
Seema Nayak, Madhu Mati Goel, Vikram Bhatia, Saumya Chandra, Annu Makker, Sandeep Kumar, Satya Prakash Agrawal, Divya Mehrotra, Srikanta Kumar Rath
BACKGROUND: Decorin is an extracellular matrix, multifunctional small proteoglycan molecule in tumor stroma that has been shown to be modulator of angiogenesis. No clinical data is available so far on decorin expression and survival outcome of oral cancer. AIM: The aim of the present study was to examine molecular and phenotypic expression of two angiogenesis modulators viz. decorin and vascular endothelial growth factor-A (VEGF-A) in human potentially malignant oral lesions (PMOLs) and oral squamous cell carcinomas (OSCC) in relation to clinico-pathological variables and survival outcome...
July 2013: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/24148022/the-novel-heparin-binding-motif-in-decorin-binding-protein-a-from-strain-b31-of-borrelia-burgdorferi-explains-the-higher-binding-affinity
#16
Ashli Morgan, Xu Wang
Decorin-binding protein A (DBPA), a glycosaminoglycan (GAG)-binding lipoprotein found in Borrelia burgdorferi, is crucial to the transmission of Lyme disease in its earliest stages. Because of its role in the initial transmission of the disease, DBPA is an ideal target for vaccine development. DBPA sequences from different strains also contain considerable heterogeneity, leading to differing affinities for GAGs and proteoglycans among different DBPA sequences. Through biophysical and structural analysis of DBPA from strain B31, we have discovered a novel and important GAG-binding epitope in B31 DBPA...
November 19, 2013: Biochemistry
https://www.readbyqxmd.com/read/23867131/a-comparison-of-the-behavioral-and-anatomical-outcomes-in-sub-acute-and-chronic-spinal-cord-injury-models-following-treatment-with-human-mesenchymal-precursor-cell-transplantation-and-recombinant-decorin
#17
Stuart I Hodgetts, Paul J Simmons, Giles W Plant
This study assessed the potential of highly purified (Stro-1(+)) human mesenchymal precursor cells (hMPCs) in combination with the anti-scarring protein decorin to repair the injured spinal cord (SC). Donor hMPCs isolated from spinal cord injury (SCI) patients were transplanted into athymic rats as a suspension graft, alone or after previous treatment with, core (decorin(core)) and proteoglycan (decorin(pro)) isoforms of purified human recombinant decorin. Decorin was delivered via mini-osmotic pumps for 14 days following sub-acute (7 day) or chronic (1 month) SCI...
October 2013: Experimental Neurology
https://www.readbyqxmd.com/read/23226541/the-dermatan-sulfate-proteoglycan-decorin-modulates-%C3%AE-2%C3%AE-1-integrin-and-the-vimentin-intermediate-filament-system-during-collagen-synthesis
#18
Oliver Jungmann, Katerina Nikolovska, Christian Stock, Jan-Niklas Schulz, Beate Eckes, Christoph Riethmüller, Rick T Owens, Renato V Iozzo, Daniela G Seidler
Decorin, a small leucine-rich proteoglycan harboring a dermatan sulfate chain at its N-terminus, is involved in regulating matrix organization and cell signaling. Loss of the dermatan sulfate of decorin leads to an Ehlers-Danlos syndrome characterized by delayed wound healing. Decorin-null (Dcn(-/-)) mice display a phenotype similar to that of EDS patients. The fibrillar collagen phenotype of Dcn(-/-) mice could be rescued in vitro by decorin but not with decorin lacking the glycosaminoglycan chain. We utilized a 3D cell culture model to investigate the impact of the altered extracellular matrix on Dcn(-/-) fibroblasts...
2012: PloS One
https://www.readbyqxmd.com/read/22507529/knockdown-of-aberrantly-expressed-nuclear-localized-decorin-attenuates-tumour-angiogenesis-related-mediators-in-oral-cancer-progression-model-in-vitro
#19
Nyla Dil, Abhijit G Banerjee
BACKGROUND: Oral cancer accounts for roughly 3% of cancer cases in the world with about 350,000 newly reported cases annually and a 5-year survival rate of only 50%. Majority of oral cancers are squamous cell carcinomas that originate in the oral mucosal epithelial linings. We have previously shown that in human malignant squamous cells carcinoma (SCC-25) as well as in dysplastic oral keratinocytes (DOK), a small leucine-rich multifunctional proteoglycan decorin is aberrantly expressed and localized in the nucleus where it interacts with nuclear epidermal growth factor receptor (EGFR)...
June 8, 2012: Head & Neck Oncology
https://www.readbyqxmd.com/read/22414830/lipopolysaccharide-enhances-decorin-expression-through-the-toll-like-receptor-4-myeloid-differentiating-factor-88-nuclear-factor-kappa-b-and-mitogen-activated-protein-kinase-pathways-in-odontoblast-cells
#20
Wenxi He, Tiejun Qu, Qing Yu, Zhihua Wang, Hanguo Wang, Jing Zhang, Anthony J Smith
INTRODUCTION: Lipopolysaccharide (LPS) has been shown to regulate the function of odontoblasts. However, the molecular mechanisms of the effect of LPS on odontoblasts are poorly understood. Decorin (DCN), one of the major matrix proteoglycans, is known to affect the mineralization of teeth. In this study, we investigated whether LPS can regulate the expression of DCN in odontoblasts and determined the intracellular signaling pathways triggered by LPS. METHODS: The DCN messenger RNA and protein expression changes in mouse odontoblast-lineage cells (OLCs) were detected by real-time polymerase chain reaction (PCR) analysis and enzyme-linked immunosorbent assay (ELISA)...
April 2012: Journal of Endodontics
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