keyword
https://read.qxmd.com/read/38138485/nucleotides-as-bacterial-second-messengers
#21
REVIEW
Mario E Cancino-Diaz, Claudia Guerrero-Barajas, Gabriel Betanzos-Cabrera, Juan C Cancino-Diaz
In addition to comprising monomers of nucleic acids, nucleotides have signaling functions and act as second messengers in both prokaryotic and eukaryotic cells. The most common example is cyclic AMP (cAMP). Nucleotide signaling is a focus of great interest in bacteria. Cyclic di-AMP (c-di-AMP), cAMP, and cyclic di-GMP (c-di-GMP) participate in biological events such as bacterial growth, biofilm formation, sporulation, cell differentiation, motility, and virulence. Moreover, the cyclic-di-nucleotides (c-di-nucleotides) produced in pathogenic intracellular bacteria can affect eukaryotic host cells to allow for infection...
December 7, 2023: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/38131902/comparison-of-yersinia-enterocolitica-dna-methylation-at-ambient-and-host-temperatures
#22
JOURNAL ARTICLE
Dustin J Van Hofwegen, Carolyn J Hovde, Scott A Minnich
Pathogenic bacteria recognize environmental cues to vary gene expression for host adaptation. Moving from ambient to host temperature, Yersinia enterocolitica responds by immediately repressing flagella synthesis and inducing the virulence plasmid (pYV)-encoded type III secretion system. In contrast, shifting from host to ambient temperature requires 2.5 generations to restore motility, suggesting a link to the cell cycle. We hypothesized that differential DNA methylation contributes to temperature-regulated gene expression...
November 30, 2023: Epigenomes
https://read.qxmd.com/read/38129386/structural-insights-into-the-regulation-ligand-recognition-and-oligomerization-of-bacterial-sting
#23
JOURNAL ARTICLE
Mei-Hui Hou, Yu-Chuan Wang, Chia-Shin Yang, Kuei-Fen Liao, Je-Wei Chang, Orion Shih, Yi-Qi Yeh, Manoj Kumar Sriramoju, Tzu-Wen Weng, U-Ser Jeng, Shang-Te Danny Hsu, Yeh Chen
The cyclic GMP-AMP synthase (cGAS)/stimulator of interferon gene (STING) signaling pathway plays a critical protective role against viral infections. Metazoan STING undergoes multilayers of regulation to ensure specific signal transduction. However, the mechanisms underlying the regulation of bacterial STING remain unclear. In this study, we determined the crystal structure of anti-parallel dimeric form of bacterial STING, which keeps itself in an inactive state by preventing cyclic dinucleotides access. Conformational transition between inactive and active states of bacterial STINGs provides an on-off switch for downstream signaling...
December 21, 2023: Nature Communications
https://read.qxmd.com/read/38129021/insights-into-the-mechanisms-and-key-factors-influencing-biofilm-formation-by-aeromonas-hydrophila-in-the-food-industry-a-comprehensive-review-and-bibliometric-analysis
#24
REVIEW
Md Ashikur Rahman, Shirin Akter, Md Ashrafudoulla, Md Anamul Hasan Chowdhury, A G M Sofi Uddin Mahamud, Si Hong Park, Sang-Do Ha
Biofilm formation by Aeromonas hydrophila in the food industry poses significant challenges to food safety and quality. Therefore, this comprehensive review aimed to provide insights into the mechanisms and key factors influencing A. hydrophila biofilm formation. It explores the molecular processes involved in initial attachment, microcolony formation, and biofilm maturation; moreover, it concurrently examines the impact of intrinsic factors, including quorum sensing, cyclic-di-GMP, the efflux pump, and antibiotic resistance, as well as environmental conditions, such as temperature, nutrient availability, and osmotic pressure, on biofilm architecture and resilience...
January 2024: Food Research International
https://read.qxmd.com/read/38112425/flhf-affects-the-subcellular-clustering-of-wspr-through-hsbr-in-pseudomonas-aeruginosa
#25
JOURNAL ARTICLE
Congcong Guan, Yi Huang, Yun Zhou, Yuqian Han, Shuhui Liu, Shimin Liu, Weina Kong, Tietao Wang, Yani Zhang
Cyclic di-GMP (c-di-GMP) is a second messenger that controls flagellum biosynthesis, adhesion, virulence, motility, exopolysaccharide production, and biofilm formation in bacteria. Recent research has shown that distinct diguanylate cyclases (DGCs) or phosphodiesterases (PDEs) produce highly specific outputs. Some DGCs and PDEs contribute to the total global c-di-GMP concentration, but others only affect local c-di-GMP in a microenvironment. However, the underlying mechanisms are unclear. Here, we report that FlhF affects the localization and DGC activity of WspR via HsbR and is implicated in local c-di-GMP signaling in Pseudomonas aeruginosa ...
December 19, 2023: Applied and Environmental Microbiology
https://read.qxmd.com/read/38095464/structure-based-virtual-screening-and-in-vitro-validation-of-inhibitors-of-cyclic-dinucleotide-phosphodiesterases-enpp1-and-cdnp
#26
JOURNAL ARTICLE
Akshay Rohilla, Alok Kumar Singh, Benjamin Koleske, Geetha Srikrishna, William R Bishai
In this paper, we describe novel inhibitors of cyclic dinucleotide phosphodiesterase enzymes from Mycobacterium tuberculosis ( M.tb ) (CdnP) and mammals (ENPP1). The phosphodiesterase enzymes hydrolyze cyclic dinucleotides, such as 2',3'-cyclic GMP-AMP and c-di-AMP, which are stimulator of interferon gene (STING) agonists. By blocking the hydrolysis of STING agonists, the cyclic GMP-AMP synthase (cGAS)-STING-IRF3 pathway is potentiated. There is strong evidence in tuberculosis and in cancer biology that potentiation of the cGAS-STING-IRF3 pathway leads to improved M...
January 11, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38051770/structures-of-the-p-aeruginosa-fleq-flen-master-regulators-reveal-large-scale-conformational-switching-in-motility-and-biofilm-control
#27
JOURNAL ARTICLE
Lucía Torres-Sánchez, Thibault Géry Sana, Marion Decossas, Yaser Hashem, Petya Violinova Krasteva
Pseudomonas aeruginosa can cause a wide array of chronic and acute infections associated with its ability to rapidly switch between planktonic, biofilm, and dispersed lifestyles, each with a specific arsenal for bacterial survival and virulence. At the cellular level, many of the physiological transitions are orchestrated by the intracellular second messenger c-di-GMP and its receptor-effector FleQ. A bacterial enhancer binding protein, FleQ acts as a master regulator of both flagellar motility and adherence factor secretion and uses remarkably different transcription activation mechanisms depending on its dinucleotide loading state, adenosine triphosphatase (ATPase) activity, interactions with polymerase sigma (σ) factors, and complexation with a second ATPase, FleN...
December 12, 2023: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38033560/host-cell-based-screening-assays-for-identification-of-molecules-targeting-pseudomonas-aeruginosa-cyclic-di-gmp-signaling-and-biofilm-formation
#28
JOURNAL ARTICLE
Ying Hu, Jeremy Stephen Webb, Shi-Qi An
The rapid emergence of bacterial resistance to antibiotics in current use is occurring worldwide and poses a significant threat to global healthcare systems. Recent research to identify new effective anti-bacterial agents has focused on regulatory pathways as targets for interference. Regulatory mechanisms employing intracellular Bis-(3',5') cyclic di-guanylate (c-di-GMP) as a secondary messenger represent a distinct category of subjects. This molecule, c-di-GMP, is present in nearly all bacterial species and plays a pivotal role in governing various biological processes, encompassing antibiotic resistance, biofilm formation, and virulence...
2023: Frontiers in Microbiology
https://read.qxmd.com/read/38014955/-cis-da-dependent-dispersion-by-pseudomonas-aeruginosa-biofilm-and-identification-of-cis-da-sensory-protein-dsps
#29
JOURNAL ARTICLE
Manmohit Kalia, Diana Amari, David G Davies, Karin Sauer
Dispersion is an essential stage of the biofilm life cycle resulting in the release of bacteria from a biofilm into the surrounding environment. Dispersion contributes to bacterial survival by relieving overcrowding within a biofilm and allowing dissemination of cells into new habitats for colonization. Thus, dispersion can contribute to biofilm survival as well as disease progression and transmission. Cells dispersed from a biofilm rapidly lose their recalcitrant antimicrobial-tolerant biofilm phenotype and transition to a state that is susceptible to antibiotics...
November 28, 2023: MBio
https://read.qxmd.com/read/38014003/single-phage-proteins-sequester-tir-and-cgas-generated-signaling-molecules
#30
Dong Li, Yu Xiao, Weijia Xiong, Iana Fedorova, Yu Wang, Xi Liu, Erin Huiting, Jie Ren, Zirui Gao, Xingyu Zhao, Xueli Cao, Yi Zhang, Joseph Bondy-Denomy, Yue Feng
Prokaryotic anti-phage immune systems use TIR (toll/interleukin-1 receptor) and cGAS (cyclic GMP-AMP synthase) enzymes to produce 1''-3'/1''-2' glycocyclic ADPR (gcADPR) and cyclid di-/tri-nucleotides (CDNs and CTNs) signaling molecules that limit phage replication, respectively 1-3 . However, how phages neutralize these common systems is largely unknown. Here, we show that T hoeris a nti- d efense proteins Tad1 4 and Tad2 5 both have anti-CBASS activity by simultaneously sequestering CBASS cyclic oligonucleotides...
November 16, 2023: bioRxiv
https://read.qxmd.com/read/38000372/cyclic-di-gmp-inhibits-nitrate-assimilation-by-impairing-the-antitermination-function-of-nast-in-pseudomonas-putida
#31
JOURNAL ARTICLE
Liang Nie, Yujie Xiao, Tiantian Zhou, Haoqi Feng, Meina He, Qingyuan Liang, Kexin Mu, Hailing Nie, Qiaoyun Huang, Wenli Chen
The ubiquitous bacterial second messenger cyclic diguanylate (c-di-GMP) coordinates diverse cellular processes through its downstream receptors. However, whether c-di-GMP participates in regulating nitrate assimilation is unclear. Here, we found that NasT, an antiterminator involved in nitrate assimilation in Pseudomonas putida, specifically bound c-di-GMP. NasT was essential for expressing the nirBD operon encoding nitrite reductase during nitrate assimilation. High-level c-di-GMP inhibited the binding of NasT to the leading RNA of nirBD operon (NalA), thus attenuating the antitermination function of NasT, resulting in decreased nirBD expression and nitrite reductase activity, which in turn led to increased nitrite accumulation in cells and its export...
November 24, 2023: Nucleic Acids Research
https://read.qxmd.com/read/37996405/regulation-of-the-physiology-and-virulence-of-ralstonia-solanacearum-by-the-second-messenger-2-3-cyclic-guanosine-monophosphate
#32
JOURNAL ARTICLE
Xia Li, Wenfang Yin, Junjie Desmond Lin, Yong Zhang, Quan Guo, Gerun Wang, Xiayu Chen, Binbin Cui, Mingfang Wang, Min Chen, Peng Li, Ya-Wen He, Wei Qian, Haibin Luo, Lian-Hui Zhang, Xue-Wei Liu, Shihao Song, Yinyue Deng
Previous studies have demonstrated that bis-(3',5')-cyclic diguanosine monophosphate (bis-3',5'-c-di-GMP) is a ubiquitous second messenger employed by bacteria. Here, we report that 2',3'-cyclic guanosine monophosphate (2',3'-cGMP) controls the important biological functions, quorum sensing (QS) signaling systems and virulence in Ralstonia solanacearum through the transcriptional regulator RSp0980. This signal specifically binds to RSp0980 with high affinity and thus abolishes the interaction between RSp0980 and the promoters of target genes...
November 23, 2023: Nature Communications
https://read.qxmd.com/read/37980414/c-di-gmp-inhibits-the-dna-binding-activity-of-h-ns-in-salmonella
#33
JOURNAL ARTICLE
Shuyu Li, Qinmeng Liu, Chongyi Duan, Jialin Li, Hengxi Sun, Lei Xu, Qiao Yang, Yao Wang, Xihui Shen, Lei Zhang
Cyclic di-GMP (c-di-GMP) is a second messenger that transduces extracellular stimuli into cellular responses and regulates various biological processes in bacteria. H-NS is a global regulatory protein that represses expression of many genes, but how H-NS activity is modulated by environmental signals remains largely unclear. Here, we show that high intracellular c-di-GMP levels, induced by environmental cues, relieve H-NS-mediated transcriptional silencing in Salmonella enterica serovar Typhimurium. We find that c-di-GMP binds to the H-NS protein to inhibit its binding to DNA, thus derepressing genes silenced by H-NS...
November 18, 2023: Nature Communications
https://read.qxmd.com/read/37942962/overview-of-the-effects-and-mechanisms-of-no-and-its-donors-on-biofilms
#34
REVIEW
Yanan Tian, Xiaojing Tian, Teng Li, Wenhang Wang
Microbial biofilm is undoubtedly a challenging problem in the food industry. It is closely associated with human health and life, being difficult to remove and antibiotic resistance. Therefore, an alternate method to solve these problems is needed. Nitric oxide (NO) as an antimicrobial agent, has shown great potential to disrupt biofilms. However, the extremely short half-life of NO in vivo (2 s) has facilitated the development of relatively more stable NO donors. Recent studies reported that NO could permeate biofilms, causing damage to cellular biomacromolecules, inducing biofilm dispersion by quorum sensing (QS) pathway and reducing intracellular bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) levels, and significantly improving the bactericidal effect without drug resistance...
November 9, 2023: Critical Reviews in Food Science and Nutrition
https://read.qxmd.com/read/37932063/protein-containing-the-ggdef-domain-affects-motility-and-biofilm-formation-in-vibrio-cholerae-and-is-negatively-regulated-by-fur-and-hapr
#35
JOURNAL ARTICLE
He Gao, Li Zhi Ma, Qin Qin, Yao Cui, Xiao Han Ma, Yi Quan Zhang, Biao Kan
OBJECTIVE: This study aimed to investigate whether the VCA0560 gene acts as an active diguanylate cyclase (DGC) in Vibrio cholerae and how its transcription is regulated by Fur and HapR. METHODS: The roles of VCA0560 was investigated by utilizing various phenotypic assays, including colony morphological characterization, crystal violet staining, Cyclic di-GMP (c-di-GMP) quantification, and swimming motility assay. The regulation of the VCA0560 gene by Fur and HapR was analyzed by luminescence assay, electrophoretic mobility shift assay, and DNase I footprinting...
October 20, 2023: Biomedical and Environmental Sciences: BES
https://read.qxmd.com/read/37902393/autoinducer-2-promotes-adherence-of-aeromonas-veronii-through-facilitating-the-expression-of-msha-type-iv-pili-genes-mediated-by-c-di-gmp
#36
JOURNAL ARTICLE
Yi Li, Shuo Han, Yuqi Wang, Mengyuan Qin, Chengjin Lu, Yingke Ma, Wenqing Yang, Jiajia Liu, Xiaohua Xia, Hailei Wang
Aeromonas veronii. a ubiquitous of zoonotic disease pathogen, depends on adhesion as the crucial way to colonize the gastrointestinal tract of humans and animals, which further causes severe gastrointestinal diseases and parenteral infections. However, the adherence mechanism of A. veronii has not been fully characterized. Therefore, we investigate the effect of autoinducer-2 (AI-2) on adherence of A. veronii through facilitating the expression of mannose-sensitive hemagglutinin (MSHA) type IV pili genes mediated by cyclic diguanosine monophosphate (c-di-GMP)...
October 30, 2023: Applied and Environmental Microbiology
https://read.qxmd.com/read/37894891/phylogenetic-analysis-and-characterization-of-diguanylate-cyclase-and-phosphodiesterase-in-planktonic-filamentous-cyanobacterium-arthrospira-sp
#37
JOURNAL ARTICLE
Kang Wang, Wenjun Li, Hongli Cui, Song Qin
Cyclic di-GMP (c-di-GMP) is a second messenger of intracellular communication in bacterial species, which widely modulates diverse cellular processes. However, little is known about the c-di-GMP network in filamentous multicellular cyanobacteria. In this study, we preliminarily investigated the c-di-GMP turnover proteins in Arthrospira based on published protein data. Bioinformatics results indicate the presence of at least 149 potential turnover proteins in five Arthrospira subspecies. Some proteins are highly conserved in all tested Arthrospira , whereas others are specifically found only in certain subspecies...
October 16, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37894183/review-of-the-impact-of-biofilm-formation-on-recurrent-clostridioides-difficile-infection
#38
REVIEW
Daira Rubio-Mendoza, Adrián Martínez-Meléndez, Héctor Jesús Maldonado-Garza, Carlos Córdova-Fletes, Elvira Garza-González
Clostridioides difficile infection (CDI) may recur in approximately 10-30% of patients, and the risk of recurrence increases with each successive recurrence, reaching up to 65%. C. difficile can form biofilm with approximately 20% of the bacterial genome expressed differently between biofilm and planktonic cells. Biofilm plays several roles that may favor recurrence; for example, it may act as a reservoir of spores, protect the vegetative cells from the activity of antibiotics, and favor the formation of persistent cells...
October 10, 2023: Microorganisms
https://read.qxmd.com/read/37880815/regulatory-effects-of-diverse-dsf-family-quorum-sensing-signals-in-plant-associated-bacteria
#39
JOURNAL ARTICLE
Kaihuai Li, Chaoyun Ma, Xue Zhou, Chunlan Xiong, Bo Wang, Yong Wang, Fengquan Liu
Numerous bacterial species employ diffusible signal factor (DSF)-based quorum sensing (QS) as a widely conserved cell-cell signalling communication system to collectively regulate various behaviours crucial for responding to environmental changes. cis-11-Methyl-dodecenoic acid, known as DSF, was first identified as a signalling molecule in Xanthomonas campestris pv. campestris. Subsequently, many structurally related molecules have been identified in different bacterial species. This review aims to provide an overview of current understanding regarding the biosynthesis and regulatory role of DSF signals in both pathogenic bacteria and a biocontrol bacterium...
October 25, 2023: Molecular Plant-microbe Interactions: MPMI
https://read.qxmd.com/read/37873360/a-label-free-approach-for-relative-spatial-quantitation-of-c-di-gmp-in-microbial-biofilms
#40
Catherine S McCaughey, Michael A Trebino, Allyson McAtamney, Ruth Isenberg, Mark J Mandel, Fitnat H Yildiz, Laura M Sanchez
Microbial biofilms represent an important lifestyle for bacteria and are dynamic three dimensional structures. Cyclic dimeric guanosine monophosphate (c-di-GMP) is a ubiquitous signaling molecule that is known to be tightly regulated with biofilm processes. While measurements of global levels of c-di-GMP have proven valuable towards understanding the genetic control of c-di-GMP production, there is a need for tools to observe the local changes of c-di-GMP production in biofilm processes. We have developed a label-free method for the direct detection of c-di-GMP in microbial colony biofilms using matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI)...
October 10, 2023: bioRxiv
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