keyword
https://read.qxmd.com/read/38532247/a-c-di-gmp-binding-effector-stm0435-modulates-flagellar-motility-and-pathogenicity-in-salmonella
#1
JOURNAL ARTICLE
Yuanji Dai, Ruirui Liu, Yingying Yue, Nannan Song, Haihong Jia, Zhongrui Ma, Xueyan Gao, Min Zhang, Xilu Yuan, Qing Liu, Xiaoyu Liu, Bingqing Li, Weiwei Wang
Flagella play a crucial role in the invasion process of Salmonella and function as a significant antigen that triggers host pyroptosis. Regulation of flagellar biogenesis is essential for both pathogenicity and immune escape of Salmonella . We identified the conserved and unknown function protein STM0435 as a new flagellar regulator. The ∆ stm0435 strain exhibited higher pathogenicity in both cellular and animal infection experiments than the wild-type Salmonella . Proteomic and transcriptomic analyses demonstrated dramatic increases in almost all flagellar genes in the ∆ stm0435 strain compared to wild-type Salmonella ...
December 2024: Virulence
https://read.qxmd.com/read/38527857/borrelia-burgdorferi-plza-is-a-cyclic-di-gmp-dependent-dna-and-rna-binding-protein
#2
JOURNAL ARTICLE
Nerina Jusufovic, Andrew C Krusenstjerna, Christina R Savage, Timothy C Saylor, Catherine A Brissette, Wolfram R Zückert, Paula J Schlax, Md A Motaleb, Brian Stevenson
The PilZ domain-containing protein, PlzA, is the only known cyclic di-GMP binding protein encoded by all Lyme disease spirochetes. PlzA has been implicated in the regulation of many borrelial processes, but the effector mechanism of PlzA was not previously known. Here, we report that PlzA can bind DNA and RNA and that nucleic acid binding requires c-di-GMP, with the affinity of PlzA for nucleic acids increasing as concentrations of c-di-GMP were increased. A mutant PlzA that is incapable of binding c-di-GMP did not bind to any tested nucleic acids...
March 25, 2024: Molecular Microbiology
https://read.qxmd.com/read/38522512/dynamics-driven-allosteric-stimulation-of-diguanylate-cyclase-activity-in-a-red-light-regulated-phytochrome
#3
JOURNAL ARTICLE
Quang Hieu Tran, Oliver Maximilian Eder, Andreas Winkler
Sensor-effector proteins integrate information from different stimuli and transform this into cellular responses. Some sensory domains, like red-light responsive bacteriophytochromes, show a remarkable modularity regulating a variety of effectors. One effector domain is the GGDEF diguanylate cyclase catalysing the formation of the bacterial second messenger cyclic-dimeric-guanosine monophosphate. While critical signal integration elements have been described for different phytochromes, a generalized understanding of signal processing and communication over large distances, roughly 100 Angstrom in phytochrome diguanylate cyclases, is missing...
March 22, 2024: Journal of Biological Chemistry
https://read.qxmd.com/read/38521769/a-genetic-screen-identifies-a-role-for-oprf-in-pseudomonas-aeruginosa-biofilm-stimulation-by-subinhibitory-antibiotics
#4
JOURNAL ARTICLE
Luke N Yaeger, Michael R M Ranieri, Jessica Chee, Sawyer Karabelas-Pittman, Madeleine Rudolph, Alessio M Giovannoni, Hanjeong Harvey, Lori L Burrows
Biofilms are surface-associated communities of bacteria that grow in a self-produced matrix of polysaccharides, proteins, and extracellular DNA (eDNA). Sub-minimal inhibitory concentrations (sub-MIC) of antibiotics induce biofilm formation, potentially as a defensive response to antibiotic stress. However, the mechanisms behind sub-MIC antibiotic-induced biofilm formation are unclear. We show that treatment of Pseudomonas aeruginosa with multiple classes of sub-MIC antibiotics with distinct targets induces biofilm formation...
March 23, 2024: NPJ Biofilms and Microbiomes
https://read.qxmd.com/read/38511653/gene-expression-reprogramming-of-pseudomonas-alloputida-in-response-to-arginine-through-the-transcriptional-regulator-argr
#5
JOURNAL ARTICLE
María Antonia Molina-Henares, María Isabel Ramos-González, Serena Rinaldo, Manuel Espinosa-Urgel
Different bacteria change their life styles in response to specific amino acids. In Pseudomonas putida (now alloputida ) KT2440, arginine acts both as an environmental and a metabolic indicator that modulates the turnover of the intracellular second messenger c-di-GMP, and expression of biofilm-related genes. The transcriptional regulator ArgR, belonging to the AraC/XylS family, is key for the physiological reprogramming in response to arginine, as it controls transport and metabolism of the amino acid. To further expand our knowledge on the roles of ArgR, a global transcriptomic analysis of KT2440 and a null argR mutant growing in the presence of arginine was carried out...
March 2024: Microbiology
https://read.qxmd.com/read/38498718/reconstitution-of-a-biofilm-adhesin-system-from-a-sulfate-reducing-bacterium-in-pseudomonas-fluorescens
#6
JOURNAL ARTICLE
Amruta A Karbelkar, Maria Font, T Jarrod Smith, Holger Sondermann, George A O'Toole
Biofilms of sulfate-reducing bacterium (SRB) like Desulfovibrio vulgaris Hildenborough (DvH) can facilitate metal corrosion in various industrial and environmental settings leading to substantial economic losses. Although the mechanisms of biofilm formation by DvH are not yet well understood, recent studies indicate the large adhesin, DvhA, is a key determinant of biofilm formation. The dvhA gene neighborhood resembles the biofilm-regulating Lap system of Pseudomonas fluorescens but is curiously missing the c-di-GMP-binding regulator LapD...
March 26, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38452551/evfg-is-a-multi-function-protein-located-in-the-type-vi-secretion-system-for-expec
#7
JOURNAL ARTICLE
Wenjia Lu, Hao Lu, Xinyu Huo, Chenchen Wang, Zhaoran Zhang, Bingbing Zong, Gaoyan Wang, Wenqi Dong, Xiaodan Li, Yuying Li, Huanchun Chen, Chen Tan
The Type VI secretion system (T6SS) functions as a protein transport nanoweapon in several stages of bacterial life. Even though bacterial competition is the primary function of T6SS, different bacteria exhibit significant variations. Particularly in Extraintestinal pathogenic Escherichia coli (ExPEC), research into T6SS remains relatively limited. This study identified the uncharacterized gene evfG within the T6SS cluster of ExPEC RS218. Through our experiments, we showed that evfG is involved in T6SS expression in ExPEC RS218...
February 23, 2024: Microbiological Research
https://read.qxmd.com/read/38436566/flip-the-switch-the-role-of-fleq-in-modulating-the-transition-between-the-free-living-and-sessile-mode-of-growth-in-pseudomonas-aeruginosa
#8
REVIEW
Victoria I Oladosu, Soyoung Park, Karin Sauer
Pseudomonas aeruginosa is a Gram-negative, opportunistic pathogen causing chronic infections that are associated with the sessile/biofilm mode of growth rather than the free-living/planktonic mode of growth. The transcriptional regulator FleQ contributes to both modes of growth by functioning both as an activator and repressor and inversely regulating flagella genes associated with the planktonic mode of growth and genes contributing to the biofilm mode of growth. Here, we review findings that enhance our understanding of the molecular mechanism by which FleQ enables the transition between the two modes of growth...
March 4, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38431313/biofilm-formation-of-hafnia-paralvei-induced-by-c-di-gmp-through-facilitating-bcsb-gene-expression-promotes-spoilage-of-yellow-river-carp-cyprinus-carpio
#9
JOURNAL ARTICLE
Mengyuan Qin, Shuo Han, Miaomiao Chen, Pengju Li, Yuqi Wang, Wenfang Niu, Chao Gao, Hailei Wang, Yi Li
Hafnia paralvei, a Gram-negative foodborne pathogen, is found ubiquitously in various aquatic animals and seafoods, which can form biofilm as a dominant virulence factor that contributes to its pathogenesis. However, the biofilm formation mechanism of H. paralvei and its effect on food spoilage has not been fully characterized. Here we show that biofilm formation, is regulated by c-di-GMP which mediated by bcsB, can increase the spoilage ability of H. paralvei. We found that GTP was added exogenously to enhance the synthesis of c-di-GMP, which further promoted biofilm formation...
June 2024: Food Microbiology
https://read.qxmd.com/read/38429473/exploring-the-metabolic-response-of-pseudomonas-putida-to-l-arginine
#10
JOURNAL ARTICLE
Chiara Scribani-Rossi, María Antonia Molina-Henares, Manuel Espinosa-Urgel, Serena Rinaldo
Beyond their role as protein-building units, amino acids are modulators of multiple behaviours in different microorganisms. In the root-colonizing beneficial bacterium Pseudomonas putida (recently proposed to be reclassified as alloputida) KT2440, current evidence suggests that arginine functions both as a metabolic indicator and as an environmental signal molecule, modulating processes such as chemotactic responses, siderophore-mediated iron uptake or the levels of the intracellular second messenger cyclic diguanylate (c-di-GMP)...
March 2, 2024: Advances in Experimental Medicine and Biology
https://read.qxmd.com/read/38424057/a-c-di-gmp-signaling-module-controls-responses-to-iron-in-pseudomonas-aeruginosa
#11
JOURNAL ARTICLE
Xueliang Zhan, Kuo Zhang, Chenchen Wang, Qiao Fan, Xiujia Tang, Xi Zhang, Ke Wang, Yang Fu, Haihua Liang
Cyclic dimeric guanosine monophosphate (c-di-GMP) serves as a bacterial second messenger that modulates various processes including biofilm formation, motility, and host-microbe symbiosis. Numerous studies have conducted comprehensive analysis of c-di-GMP. However, the mechanisms by which certain environmental signals such as iron control intracellular c-di-GMP levels are unclear. Here, we show that iron regulates c-di-GMP levels in Pseudomonas aeruginosa by modulating the interaction between an iron-sensing protein, IsmP, and a diguanylate cyclase, ImcA...
February 29, 2024: Nature Communications
https://read.qxmd.com/read/38403404/initial-cyclic-di-gmp-upregulation-triggers-sporadic-cellular-expansion-leading-to-improved-cellular-survival
#12
JOURNAL ARTICLE
Yongshuai Yang, Siyu Guo, Can-Jian Hong, Zhao-Xun Liang, Chun Loong Ho
Bacterial second messenger c-di-GMP upregulation is associated with the transition from planktonic to sessile microbial lifestyle, inhibiting cellular motility, and virulence. However, in-depth elucidation of the cellular processes resulting from c-di-GMP upregulation has not been fully explored. Here, we report the role of upregulated cellular c-di-GMP in promoting planktonic cell growth of Escherichia coli K12 and Pseudomonas aeruginosa PAO1. We found a rapid expansion of cellular growth during initial cellular c-di-GMP upregulation, resulting in a larger planktonic bacterial population...
February 2024: Biotechnology Journal
https://read.qxmd.com/read/38395232/the-second-messenger-cyclic-diguanylate-and-autoinducer-2-promotes-n-acylated-homoserine-lactones-based-quorum-sensing-for-enhanced-recovery-of-stored-aerobic-granular-sludge
#13
JOURNAL ARTICLE
Yi Lv, Xin Huang, Duu-Jong Lee
Efficient quorum sensing (QS) response is the premise for recovering the activities of stored aerobic granular sludge (AGS). This study aims to explore the crosstalk between the secondary messenger and the N-acylated-homoserine lactones (AHLs) to yield protein-rich granules efficiently from stored AGS by enhancing its QS efficiency selectively. 80 nmol/L cyclic diguanylate (c-di-GMP) with 20 nmol/L AHLs could increase the activity of isocitrate lyase activity (ICD) by 89 % and isocitrate dehydrogenase activity (ICDHc) by 113...
February 21, 2024: Bioresource Technology
https://read.qxmd.com/read/38370152/fluoropyrimidines-affect-de-novo-pyrimidine-synthesis-impairing-biofilm-formation-in-escherichia-coli
#14
JOURNAL ARTICLE
Srikanth Ravishankar, Valerio Baldelli, Carlo Angeletti, Nadia Raffaelli, Paolo Landini, Elio Rossi
Antivirulence agents are considered a promising strategy to treat bacterial infections. Fluoropyrimidines possess antivirulence and antibiofilm activity against Gram-negative bacteria; however, their mechanism of action is yet unknown. Consistent with their known antibiofilm activity, fluoropyrimidines, particularly 5-fluorocytosine (5-FC), impair curli-dependent surface adhesion by Escherichia coli MG1655 via downregulation of curli fimbriae gene transcription. Curli inhibition requires fluoropyrimidine conversion into fluoronucleotides and is not mediated by c -di-GMP or the ymg-rcs envelope stress response axis, previously suggested as the target of fluorouracil antibiofilm activity in E...
June 2024: Biofilm
https://read.qxmd.com/read/38360477/erratum-to-bcg%C3%AE-bcg1419c-and-bcg-differ-in-induction-of-autophagy-c-di-gmp-content-proteome-and-progression-of-lung-pathology-in-mycobacterium-tuberculosis-hn878-infected-male-balb-c-mice-vaccine-41-26-2023-3824-3835
#15
Michel de Jesús Aceves-Sánchez, Jorge Alberto Barrios-Payán, Cristian Alfredo Segura-Cerda, Mario Alberto Flores-Valdez, Dulce Mata-Espinosa, César Pedroza-Roldán, Rahul Yadav, Deepak Kumar Saini, Miguel Angel de la Cruz, Miguel A Ares, Helle Bielefeldt-Ohmann, Guillermina Baay-Guzmán, Isabelle Vergne, Jesús Bernardino Velázquez-Fernández, Jeannette Barba León, Rogelio Hernández-Pando
No abstract text is available yet for this article.
February 14, 2024: Vaccine
https://read.qxmd.com/read/38357352/aerobic-granular-sludge-for-complex-heavy-metal-containing-wastewater-treatment-characterization-performance-and-mechanisms-analysis
#16
JOURNAL ARTICLE
Chong Liu, Yao Shen, Yuguang Li, Fengguang Huang, Shuo Wang, Ji Li
Complex heavy metal (HM)-containing wastewater discharges pose substantial risks to global water ecosystems and human health. Aerobic granular sludge (AGS) has attracted increased attention as an efficient and low-cost adsorbent in HM-containing wastewater treatment. Therefore, this study systematically evaluates the effect of Cu(II), Ni(II), and Cr(III) addition on the characteristics, performance and mechanism of AGS in complex HM-containing wastewater treatment process by means of fourier transform infrared spectroscopy, inductively coupled plasma spectrocopcy, confocal laser scanning microscopy, extracellular polymeric substances (EPS) fractions detection and scanning electron microscope-energy dispersive X-ray...
2024: Frontiers in Microbiology
https://read.qxmd.com/read/38340793/a-pilz-domain-protein-interacts-with-the-transcriptional-regulator-hink-to-regulate-type-vi-secretion-system-in-pseudomonas-aeruginosa
#17
JOURNAL ARTICLE
Tianfang Cheng, Qing Wei Cheang, Linghui Xu, Shuo Sheng, Zhaoting Li, Yu Shi, Huiyan Zhang, Li Mei Pang, Ding Xiang Liu, Liang Yang, Zhao-Xun Liang, Junxia Wang
Type VI secretion systems (T6SS) are bacterial macromolecular complexes that secrete effector proteins into target cells or the extracellular environment, leading to the demise of adjacent cells and providing a survival advantage. Although studies have shown that the T6SS in Pseudomonas aeruginosa is regulated by the Quorum Sensing (QS) system and secondary messenger c-di-GMP, the underlying molecular mechanism remains largely unknown. In this study, we discovered that the c-di-GMP-binding adaptor protein PA0012 has a repressive effect on expression of the T6SS HSI-I genes in Pseudomonas aeruginosa PAO1...
February 8, 2024: Journal of Biological Chemistry
https://read.qxmd.com/read/38333580/role-of-extracellular-matrix-components-in-biofilm-formation-and-adaptation-of-pseudomonas-ogarae-f113-to-the-rhizosphere-environment
#18
JOURNAL ARTICLE
Esther Blanco-Romero, Daniel Garrido-Sanz, David Durán, Morten Rybtke, Tim Tolker-Nielsen, Miguel Redondo-Nieto, Rafael Rivilla, Marta Martín
Regulating the transition of bacteria from motile to sessile lifestyles is crucial for their ability to compete effectively in the rhizosphere environment. Pseudomonas are known to rely on extracellular matrix (ECM) components for microcolony and biofilm formation, allowing them to adapt to a sessile lifestyle. Pseudomonas ogarae F113 possesses eight gene clusters responsible for the production of ECM components. These gene clusters are tightly regulated by AmrZ, a major transcriptional regulator that influences the cellular levels of c-di-GMP...
2024: Frontiers in Microbiology
https://read.qxmd.com/read/38329362/erratum-for-sun-et-al-control-of-cell-size-by-c-di-gmp-requires-a-two-component-signaling-system-in-the-cyanobacterium-anabaena-sp-strain-pcc-7120
#19
JOURNAL ARTICLE
Qing-Xue Sun, Min Huang, Ju-Yuan Zhang, Xiaoli Zeng, Cheng-Cai Zhang
No abstract text is available yet for this article.
February 8, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38290207/astragaloside-iv-alleviates-macrophage-senescence-and-d-galactose-induced-bone-loss-in-mice-through-sting-nf-%C3%AE%C2%BAb-pathway
#20
JOURNAL ARTICLE
Muzhe Li, Yuanyuan Niu, Linkun Tian, Tianchi Zhang, Shijie Zhou, Lining Wang, Jie Sun, Taxi Wumiti, Zhiwei Chen, Qinfeng Zhou, Yong Ma, Yang Guo
BACKGROUND: Senile osteoporosis (SOP) is an age-related metabolic bone disease that currently lacks specific therapeutic interventions. Thus, this study aimed to investigate the effect of Astragaloside IV (AS-IV) on macrophage senescence, bone marrow mesenchymal stem cell (BMSC) osteogenesis, and SOP progression. METHODS: A senescent macrophage model was established and treated with varying concentrations of AS-IV. Cell activity was measured using the CCK8 assay...
January 29, 2024: International Immunopharmacology
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