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https://www.readbyqxmd.com/read/28634640/the-microbiome-of-eucalyptus-roots-under-different-management-conditions-and-its-potential-for-biological-nitrogen-fixation
#1
Eduardo da Siva Fonseca, Raquel Silva Peixoto, Alexandre Soares Rosado, Fabiano de Carvalho Balieiro, James M Tiedje, Caio Tavora Coelho da Costa Rachid
Eucalyptus plantations offer a cost-effective and renewable source of raw material. There is substantial interest in improving forestry production, especially through sustainable strategies such as the use of plant growth-promoting bacteria. However, little is known about Eucalyptus microbiology. In this study, the endophytic bacterial community was assessed in Eucalyptus urograndis roots using culture-dependent and culture-independent techniques with plants grown under different conditions. Three phyla accounted for approximately 95% of the community, with Actinobacteria corresponding to approximately 59%...
June 21, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28634144/development-of-a-multiplex-pcr-assay-for-the-detection-and-differentiation-of-burkholderia-pseudomallei-burkholderia-mallei-burkholderia-thailandensis-and-burkholderia-cepacia-complex
#2
Irina Zakharova, Natalya Teteryatnikova, Andrey Toporkov, Dmitry Viktorov
Two species of Burkholderia pseudomallei complex (Bpc), B. pseudomallei and B. mallei, can cause severe life-threatening infections. Rapidly discerning individual species within the group and separating them from other opportunistic pathogens of the Burkholderia cepacia complex (Bcc) is essential to establish a correct diagnosis and for epidemiological surveillance. In this study, a multiplex PCR assay based on the detection of an individual set of chromosomal beta-lactamase genes for single-step identification and differentiation of B...
June 17, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28631632/risk-of-cardiac-pacemaker-pocket-infection-in-a-tertiary-care-hospital
#3
Jaswinder Singh Gill, Navreet Singh, S P Khanna
BACKGROUND: The risk of pacemaker pocket infections (PPIs) is rare with good antisepsis techniques and use of advanced antibiotics. However, injudicious antibiotic usage leads to the rise of multidrug-resistant bacteria, which may cause PPI. Few reports exist about the microbial spectrum of the PPI from our country, prompting us to study the same. METHODS: We conducted this retrospective observational study for 3 years (January 2013-February 2016) from all the patients with PPI...
April 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28630181/in-vitro-activity-of-the-siderophore-cephalosporin-cefiderocol-against-a-recent-collection-of-clinically-relevant-gram-negative-bacilli-from-north-america-and-europe-including-carbapenem-non-susceptible-isolates-the-sidero-wt-2014-study
#4
Meredith A Hackel, Masakatsu Tsuji, Yoshinori Yamano, Roger Echols, James A Karlowsky, Daniel F Sahm
Cefiderocol (formerly S-649266) is an investigational siderophore cephalosporin. Iron-depleted cation-adjusted Mueller-Hinton broth (ID-CAMHB) was prepared according to the Clinical and Laboratory Standards Institute (CLSI) protocol and used to perform broth microdilution testing of cefiderocol against a 2014-2015 collection of clinical isolates of gram-negative bacilli from North America (n=4,239) and Europe (n=4,966). The concentration of cefiderocol inhibiting 90% of isolates tested (MIC90) was: 0.5 (North America; n=3,007) and 1 μg/ml (Europe; n=3,080) for all isolates of Enterobacteriaceae; 1 (North America; n=30) and 4 μg/ml (Europe; n=139) for meropenem-non-susceptible (MIC ≥2 μg/ml) isolates of Enterobacteriaceae; 0...
June 19, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28628607/host-gene-expression-analysis-in-sri-lankan-melioidosis-patients
#5
Shivankari Krishnananthasivam, Nimanthi Jayathilaka, Harindra Darshana Sathkumara, Enoka Corea, Mohan Natesan, Aruna Dharshan De Silva
BACKGROUND: Melioidosis is a life threatening infectious disease caused by the gram-negative bacillus Burkholderia pseudomallei predominantly found in southeast Asia and northern Australia. Studying the host transcription profiles in response to infection is crucial for understanding disease pathogenesis and correlates of disease severity, which may help improve therapeutic intervention and survival. The aim of this study was to analyze gene expression levels of human host factors in melioidosis patients and establish useful correlation with disease biomarkers, compared to healthy individuals and patients with sepsis caused by other pathogens...
June 19, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28628442/phylogeography-of-burkholderia-pseudomallei-isolates-western-hemisphere
#6
Jay E Gee, Christopher A Gulvik, Mindy G Elrod, Dhwani Batra, Lori A Rowe, Mili Sheth, Alex R Hoffmaster
The bacterium Burkholderia pseudomallei causes melioidosis, which is mainly associated with tropical areas. We analyzed single-nucleotide polymorphisms (SNPs) among genome sequences from isolates of B. pseudomallei that originated in the Western Hemisphere by comparing them with genome sequences of isolates that originated in the Eastern Hemisphere. Analysis indicated that isolates from the Western Hemisphere form a distinct clade, which supports the hypothesis that these isolates were derived from a constricted seeding event from Africa...
July 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28625994/a-small-protein-hemp-is-a-transcriptional-activator-for-hemin-uptake-operon-in-burkholderia-multivorans-atcc-17616
#7
Takuya Sato, Shouta Nonoyama, Akane Kimura, Yuji Nagata, Yoshiyuki Ohtsubo, Masataka Tsuda
Iron and heme play very important roles in various metabolic functions in bacteria, and their intracellular homeostasis is maintained because high concentrations of free forms of these molecules greatly facilitate the Fenton reaction-mediated production of large amounts of reactive oxygen species that severely damage various biomolecules. Ferric uptake regulator (Fur) from Burkholderiamultivorans ATCC 17616 is an iron-responsive global transcriptional regulator, and its fur deletant exhibits pleiotropic phenotypes...
June 16, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28614901/x-ray-crystal-structures-of-the-pheromone-binding-domains-of-two-quorum-hindered-transcription-factors-yenr-of-yersinia-enterocolitica-and-cepr2-of-burkholderia-cenocepacia
#8
Youngchang Kim, Gekleng Chhor, Ching-Sung Tsai, Gabriel Fox, Chia-Sui Chen, Nathan J Winans, Robert Jedrzejczak, Andrzej Joachimiak, Stephen C Winans
The ability of LuxR-type proteins to regulate transcription is controlled by bacterial pheromones, N-acylhomoserine lactones (AHLs). Most LuxR-family proteins require their cognate AHLs for activity, and some of them require AHLs for folding and stability, and for protease-resistance. However, a few members of this family are able to fold, dimerize, bind DNA, and regulate transcription in the absence of AHLs; moreover, these proteins are antagonized by their cognate AHLs. One such protein is YenR of Yersinia enterocolitica, which is antagonized by N-3-oxohexanoyl-l-homoserine lactone (OHHL)...
June 14, 2017: Proteins
https://www.readbyqxmd.com/read/28599008/pediatric-melioidosis-in-sarawak-malaysia-epidemiological-clinical-and-microbiological-characteristics
#9
Anand Mohan, Yuwana Podin, Nickson Tai, Chae-Hee Chieng, Vanessa Rigas, Barbara Machunter, Mark Mayo, Desiree Wong, Su-Lin Chien, Lee-See Tan, Charles Goh, Reginal Bantin, Alexander Mijen, Wen-Yi Chua, King-Ching Hii, See-Chang Wong, Hie-Ung Ngian, Jin-Shyan Wong, Jamilah Hashim, Bart J Currie, Mong-How Ooi
BACKGROUND: Melioidosis is a serious, and potentially fatal community-acquired infection endemic to northern Australia and Southeast Asia, including Sarawak, Malaysia. The disease, caused by the usually intrinsically aminoglycoside-resistant Burkholderia pseudomallei, most commonly affects adults with predisposing risk factors. There are limited data on pediatric melioidosis in Sarawak. METHODS: A part prospective, part retrospective study of children aged <15 years with culture-confirmed melioidosis was conducted in the 3 major public hospitals in Central Sarawak between 2009 and 2014...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28596557/burkholderia-pseudomallei-in-a-lowland-rice-paddy-seasonal-changes-and-influence-of-soil-depth-and-physico-chemical-properties
#10
L Manivanh, A Pierret, S Rattanavong, O Kounnavongsa, Y Buisson, I Elliott, J -L Maeght, K Xayyathip, J Silisouk, M Vongsouvath, R Phetsouvanh, P N Newton, G Lacombe, O Ribolzi, E Rochelle-Newall, D A B Dance
Melioidosis, a severe infection with the environmental bacterium Burkholderia pseudomallei, is being recognised increasingly frequently. What determines its uneven distribution within endemic areas is poorly understood. We cultured soil from a rice field in Laos for B. pseudomallei at different depths on 4 occasions over a 13-month period. We also measured physical and chemical parameters in order to identify associated characteristics. Overall, 195 of 653 samples (29.7%) yielded B. pseudomallei. A higher prevalence of B...
June 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28596407/complete-genome-sequence-of-burkholderia-cenocepacia-cr318-a-phosphate-solubilizing-bacterium-isolated-from-corn-root
#11
Filip Zekic, Brian Weselowski, Ze-Chun Yuan
Here, we report the complete genome sequence of the phosphate-solubilizing bacterium Burkholderia cenocepacia CR318, consisting of three circular chromosomes of 3,511,146 bp, 3,097,552 bp, and 1,056,069 bp. The data presented will facilitate further insight into the mechanisms of phosphate solubilization and its application for agricultural and ecological sustainability.
June 8, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28593045/virulence-traits-associated-with-burkholderia-cenocepacia-st856-epidemic-strain-isolated-from-cystic-fibrosis-patients
#12
Milka Malešević, Zorica Vasiljević, Aleksandar Sovtić, Brankica Filipić, Katarina Novović, Milan Kojić, Branko Jovčić
BACKGROUND: Burkholderia cenocepacia is considered one of the most problematic cystic fibrosis (CF) pathogens. Colonization prevalence in the Serbian CF population is high and virtually exclusively limited to a single highly transmissible clone of B. cenocepacia ST856 which is positive for both the B. cepacia epidemic strain marker (BCESM) and cable pilin, and is closely related to the epidemic strain CZ1 (ST32). METHODS: Biofilm formation for 182 isolates, and adhesion to components of the host extracellular matrix, proteolytic activity, mucoidy and motility of selected ST856 representatives, as well as B...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28592860/role-of-flagella-and-type-four-pili-in-the-co-migration-of-burkholderia-terrae-bs001-with-fungal-hyphae-through-soil
#13
Pu Yang, Miaozhi Zhang, Jan Dirk van Elsas
Burkholderia terrae BS001 has previously been found to be able to disperse along with growing fungal hyphae in soil, with the type-3 secretion system having a supportive role in this movement. In this study, we focus on the role of two motility- and adherence-associated appendages, i.e. type-4 pili (T4P) and flagella. Electron microcopy and motility testing revealed that strain BS001 produces polar flagella and can swim on semi-solid R2A agar. Flagellum- and T4P-negative mutants were then constructed to examine the ecological roles of the respective systems...
June 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28592242/an-avirulent-burkholderia-pseudomallei-%C3%A2-purm-strain-with-atypical-type-b-lps-expansion-of-the-toolkit-for-biosafe-studies-of-melioidosis
#14
Michael H Norris, Md Siddiqur Rahman Khan, Herbert P Schweizer, Apichai Tuanyok
BACKGROUND: The work was undertaken to expand the tools available for researching Burkholderia pseudomallei (Bp), the etiological agent of the tropical disease melioidosis. Melioidosis has the potential to pose a severe threat to public health and safety. In the United States, Bp is listed as a Tier-1 select agent by the Centers for Disease Control and Prevention (CDC), thus requiring high levels of regulation and biosafety level 3 (BSL3) facilities for experimental manipulation of live organisms...
June 7, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28587856/optimization-and-characterization-of-a-murine-lung-infection-model-for-the-evaluation-of-novel-therapeutics-against-burkholderia-cenocepacia
#15
Bieke Vanhoutte, Davie Cappoen, Bidart de Macedo Maira, Freya Cools, Eveline Torfs, Tom Coenye, Wim Martinet, Guy Caljon, Louis Maes, Peter Delputte, Paul Cos
Several B. cenocepacia mouse models are available to study the pulmonary infection by this Burkholderia cepacia complex (BCC) species. However, a characterized B. cenocepacia mouse model to evaluate the efficacy of potential new antibacterial therapies is not yet described. Therefore, we optimized and validated the course of infection (i.e. bacterial proliferation in lung, liver and spleen) and the efficacy of a reference antibiotic, tobramycin (TOB), in a mouse lung infection model. Furthermore, the local immune response and histological changes in lung tissue were studied during infection and treatment...
June 3, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28584102/crystal-structures-of-the-burkholderia-multivorans-hopanoid-transporter-hpnn
#16
Nitin Kumar, Chih-Chia Su, Tsung-Han Chou, Abhijith Radhakrishnan, Jared A Delmar, Kanagalaghatta R Rajashankar, Edward W Yu
Strains of the Burkholderia cepacia complex (Bcc) are Gram-negative opportunisitic bacteria that are capable of causing serious diseases, mainly in immunocompromised individuals. Bcc pathogens are intrinsically resistant to multiple antibiotics, including β-lactams, aminoglycosides, fluoroquinolones, and polymyxins. They are major pathogens in patients with cystic fibrosis (CF) and can cause severe necrotizing pneumonia, which is often fatal. Hopanoid biosynthesis is one of the major mechanisms involved in multiple antimicrobial resistance of Bcc pathogens...
June 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28580180/the-role-of-reactive-oxygen-species-in-the-antimicrobial-activity-of-pyochelin
#17
Kuan Shion Ong, Yuen Lin Cheow, Sui Mae Lee
The increase in prevalence of antimicrobial-resistant bacteria (ARB) is currently a serious threat, thus there is a need for new antimicrobial compounds to combat infections caused by these ARB. An antimicrobial-producing bacterium, Burkholderia paludis was recently isolated and was able to produce a type of siderophore with antimicrobial properties, later identified as pyochelin. The chelating ability of pyochelin has been well-characterized but not for its antimicrobial characteristics. It was found that pyochelin had MIC values (MBC values) of 3...
July 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/28579360/complications-of-long-and-intermediate-term-venous-catheters-in-cystic-fibrosis-patients-a-multicenter-study
#18
Teresa L May, Alex H Gifford, Thomas Lahiri, Adam Black, Janet Trang, Alexandra G Cornell, Karyll Gonzalez, Scott Morin, Mark Napier, Christine W Duarte, Jonathan B Zuckerman
BACKGROUND: Totally implantable venous access devices (TIVADs) or peripherally inserted central venous catheters (PICCs) are commonly used in the care of patients with cystic fibrosis (CF), but they are associated with various complications, including thrombosis, infection, and insertion site symptoms. METHODS: We conducted a retrospective review of PICC and TIVAD use in adults and children with CF over an 8-year period at 3 accredited care centers. Patient attributes included CFTR genotype, comorbidities, lung function, body mass index, use of anticoagulation, and respiratory tract microbiology...
June 1, 2017: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
https://www.readbyqxmd.com/read/28573851/screening-of-lipases-with-unusual-high-activity-in-the-sn-2-esterification-of-1-3-dicaprin-under-mild-operating-conditions
#19
Daniel Alberto Sánchez, Gabriela Marta Tonetto, María Luján Ferreira
In this work, the synthesis of acylglycerides with high nutritional value was carried out by enzymatic esterification at sn-2 position of 1,3-dicaprin with palmitic acid. A comparative study of the performance of several biocatalysts according to the obtained products was carried out. The results obtained with several of the biocatalysts evaluated are very interesting, and it would be possible to use them to obtain a mixture of acylglycerides to act as a fat substitute. The final product was composed of about 90% of nutritionally attractive glycerides...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28567630/hot-springs-of-indian-himalayas-potential-sources-of-microbial-diversity-and-thermostable-hydrolytic-enzymes
#20
Harmesh Sahay, Ajar Nath Yadav, Atul Kumar Singh, Surendra Singh, Rajeev Kaushik, Anil Kumar Saxena
Microbial communities in hot springs at high elevations have been extensively studied worldwide. In this sense, the Indian Himalaya regions is valuable ecosystems for providing both the extreme 'cold' and 'hot' sites for exploring microbial diversity. In the present study, a total of 140 thermophilic bacteria were isolated from 12 samples collected from Manikaran and Yumthang hot springs of Indian Himalayas. The bacterial isolates were studied for phylogenetic profiling, growth properties at varying conditions and potential sources of extracellular thermostable hydrolytic enzymes such as protease, amylase, xylanase and cellulase...
June 2017: 3 Biotech
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