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Money Gupta, Rashi Chauhan, Yamuna Prasad, Gulshan Wadhwa, Chakresh Kumar Jain
The lack of complete treatments and appearance of multiple drug-resistance strains of Burkholderia cepacia complex (Bcc) are causing an increased risk of lung infections in cystic fibrosis patients. Bcc infection is a big risk to human health and demands an urgent need to identify new therapeutics against these bacteria. Network biology has emerged as one of the prospective hope in identifying novel drug targets and hits. We have applied protein-protein interaction methodology to identify new drug-target candidates (orthologs) in Burkhloderia cepacia GG4, which is an important strain for studying the quorum-sensing phenomena...
October 8, 2016: Computational Biology and Chemistry
Bartosz Roszniowski, Agnieszka Latka, Barbara Maciejewska, Dieter Vandenheuvel, Tomasz Olszak, Yves Briers, Giles S Holt, Miguel A Valvano, Rob Lavigne, Darren L Smith, Zuzanna Drulis-Kawa
Burkholderia phage AP3 (vB_BceM_AP3) is a temperate virus of the Myoviridae and the Peduovirinae subfamily (P2likevirus genus). This phage specifically infects multidrug-resistant clinical Burkholderia cenocepacia lineage IIIA strains commonly isolated from cystic fibrosis patients. AP3 exhibits high pairwise nucleotide identity (61.7 %) to Burkholderia phage KS5, specific to the same B. cenocepacia host, and has 46.7-49.5 % identity to phages infecting other species of Burkholderia. The lysis cassette of these related phages has a similar organization (putative antiholin, putative holin, endolysin, and spanins) and shows 29-98 % homology between specific lysis genes, in contrast to Enterobacteria phage P2, the hallmark phage of this genus...
October 21, 2016: Applied Microbiology and Biotechnology
Vikas Gautam, Prashant P Patil, Sunil Kumar, Samriti Midha, Mandeep Kaur, Satinder Kaur, Meenu Singh, Swapna Mali, Jayanthi Shastri, Anita Arora, Pallab Ray, Prabhu B Patil
Burkholderia cepacia complex (Bcc) is a complex group of bacteria causing opportunistic infections in immunocompromised and cystic fibrosis (CF) patients. Herein, we report multilocus sequence typing and analysis of the 57 clinical isolates of Bcc collected over the period of seven years (2005-2012) from several hospitals across India. A total of 21 sequence types (ST) including two STs from cystic fibrosis patient's isolates and twelve novel STs were identified in the population reflecting the extent of genetic diversity...
October 21, 2016: Scientific Reports
Sonja Obersteller, Heinrich Neubauer, Ralf Matthias Hagen, Hagen Frickmann
The extraction and further processing of nucleic acids (NA) from formalin-fixed paraffin-embedded (FFPE) tissues for microbiological diagnostic polymerase chain reaction (PCR) approaches is challenging. Here, we assessed the effects of five different commercially available nucleic acid extraction kits on the results of real-time PCR. FFPE samples from organs of Burkholderia pseudomallei-infected Swiss mice were subjected to processing with five different extraction kits from QIAGEN (FFPE DNA Tissue Kit, EZ1 DNA Tissue Kit, DNA Mini Kit, DNA Blood Mini Kit, and FlexiGene DNA Kit) in combination with three different real-time PCRs targeting B...
September 29, 2016: European Journal of Microbiology & Immunology
James A St John
No abstract text is available yet for this article.
October 20, 2016: Virulence
Hanan R Shehata, Cassandra L Ettinger, Jonathan A Eisen, Manish N Raizada
Endophytes are microbes that inhabit internal plant tissues without causing disease. Some endophytes are known to combat pathogens. The corn (maize) landrace Chapalote has been grown continuously by subsistence farmers in the Americas since 1000 BC, without the use of fungicides, and the crop remains highly valued by farmers, in part for its natural tolerance to pests. We hypothesized that the pathogen tolerance of Chapalote may, in part, be due to assistance from its endophytes. We previously identified a bacterial endophyte from Chapalote seeds, Burkholderia gladioli strain 3A12, for its ability to combat a diversity of crop pathogens, including Sclerotinia homoeocarpa, the most important fungal disease of creeping bentgrass, a relative of maize used here as a model system...
2016: Frontiers in Microbiology
Xiang Chen, Zhi-Qiang Liu, Chao-Ping Lin, Yu-Guo Zheng
BACKGROUND: Ethyl (R)-4-chloro-3-hydroxybutyrate ((R)-CHBE) is a versatile chiral precursor for many pharmaceuticals. Although several biosynthesis strategies have been documented to convert ethyl 4-chloro-3-oxobutanoate (COBE) to (R)-CHBE, the catalytic efficiency and stereoselectivity are still too low to be scaled up for industrial applications. Due to the increasing demand of (R)-CHBE, it is essential to explore more robust biocatalyst capable of preparing (R)-CHBE efficiently. RESULTS: A stereoselective carbonyl reductase toolbox was constructed and employed into the asymmetric reduction of COBE to (R)-CHBE...
October 18, 2016: BMC Biotechnology
R C Bernhards, C K Cote, K Amemiya, D M Waag, C P Klimko, P L Worsham, S L Welkos
Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), the agents of melioidosis and glanders, respectively, are Tier 1 biothreats. They infect humans and animals, causing disease ranging from acute and fatal to protracted and chronic. Chronic infections are especially challenging to treat, and the identification of in vitro phenotypic markers which signal progression from acute to persistent infection would be extremely valuable. First, a phenotyping strategy was developed employing colony morphotyping, chemical sensitivity testing, macrophage infection, and lipopolysaccharide fingerprint analyses to distinguish Burkholderia strains...
October 13, 2016: Archives of Microbiology
Talerngsak Kanjanabuch, Nuttha Lumlertgul, Lachlan J Pearson, Tanittha Chatsuwan, Krit Pongpirul, Asada Leelahavanichkul, Nisa Thongbor, Gunticha Nuntawong, Laksamon Praderm, Pantiwa Wechagama, Surapong Narenpitak, Apinya Wechpradit, Worauma Punya, Guttiga Halue, Phetpailin Naka, Somboon Jeenapongsa, Somchai Eiam-Ong
♦ Background: Melioidosis, an infectious disease caused by Burkholderia pseudomallei, is endemic in Southeast Asia and Northern Australia. Although a wide range of clinical manifestations from this organism are known, peritonitis associated with peritoneal dialysis (PD) has rarely been reported. ♦ Patients and Methods: Peritoneal dialysis patients from all regions in Thailand were eligible for the study if they had peritonitis and either peritoneal fluid or effluent culture positive for B.pseudomallei Patient data obtained included baseline characteristics, laboratory investigations, treatments, and clinical outcomes...
October 13, 2016: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
Elvira E Ziganshina, Dilyara M Sharifullina, Andrey P Lozhkin, Rustem N Khayrullin, Igor M Ignatyev, Ayrat M Ziganshin
Atherosclerosis is considered a chronic disease of the arterial wall and is the major cause of severe disease and death among individuals all over the world. Some recent studies have established the presence of bacteria in atherosclerotic plaque samples and suggested their possible contribution to the development of cardiovascular disease. The main objective of this preliminary pilot study was to better understand the bacterial diversity and abundance in human atherosclerotic plaques derived from common carotid arteries of individuals with atherosclerosis (Russian nationwide group) and contribute towards the further identification of a main group of atherosclerotic plaque bacteria by 454 pyrosequencing their 16S ribosomal RNA (16S rRNA) genes...
2016: PloS One
Chinn-Woan Lowe, Benjamin A Satterfield, Daniel B Nelson, Joseph D Thiriot, Michael J Heder, Jordon K March, David S Drake, Cynthia S Lew, Annette J Bunnell, Emily S Moore, Kim L O'Neill, Richard A Robison
The Burkholderia pseudomallei complex classically consisted of B. mallei, B. pseudomallei, and B. thailandensis, but has now expanded to include B. oklahomensis, B. humptydooensis, and three unassigned Burkholderia clades. Methods for detecting and differentiating the B. pseudomallei complex has been the topic of recent research due to phenotypic and genotypic similarities of these species. B. mallei and B. pseudomallei are recognized as CDC Tier 1 select agents, and are the causative agents of glanders and melioidosis, respectively...
2016: PloS One
Ujjwayini Ray, Soma Dutta, Suresh Ramasubban, Dhiman Sen, Indrajeet Kumar Tiwary
OBJECTIVE: Melioidosis caused by the Gram-negative bacterium Burkholderia pseudomallei is a very serious infection and has been sporadically reported from the Indian subcontinent. This disease entity can have acute and chronic presentations involving different organ systems. The purpose of this study is to analyze the risk factors, clinical presentations, therapy and outcome of culture proven cases of melioidosis. METHODS: We carried out a retrospective study of eight culture proven cases of melioidosis at a tertiary care hospital in West Bengal...
May 2016: Journal of the Association of Physicians of India
Elin Djurhuus Samuelsen, Nora Badawi, Ole Nybroe, Sebastian R Sørensen, Jens Aamand
Pesticide-polluted drinking water may be remediated by inoculating waterworks sand filters with specific degrading bacteria. However, degradation efficiency is often hampered by the poor adhesion behaviour of the introduced bacteria. The phenoxy acid herbicide 4-chloro-2-methyl-phenoxy-acetic acid (MCPA) is a widespread groundwater contaminant. The aim of this study was to investigate whether specific surface characteristics of MCPA-degrading bacteria could be linked to their degrading capabilities in sand filters...
October 12, 2016: Applied Microbiology and Biotechnology
Rongpong Plongla, Theofano Panagea, David H Pincus, Melissa C Jones, Peter H Gilligan
Environmental glucose non-fermenting Gram-negative bacilli other than Pseudomonas aeruginosa are frequently isolated from respiratory specimens of patients with cystic fibrosis (CF) (1).….
October 12, 2016: Journal of Clinical Microbiology
Alessandro Gori, Laura Sola, Paola Gagni, Giulia Bruni, Marta Liprino, Claudio Peri, Giorgio Colombo, Marina Cretich, Marcella Chiari
The generation of robust analytical data using microarray platforms strictly relies on optimal ligand-target interaction at the sensor surface which, in turn, is inherently bound to the correct immobilization scheme of the interrogated bioprobes. In the present work, we performed a rigorous comparative analysis of the impact of peptide ligands immobilization strategy in the screening of Burkholderia cepacia complex (BCC) infections in patients affected by cystic fibrosis (CF). We generated arrays of previously validated Burkholderia-derived peptide probes, that were selectively oriented on polymeric coatings by means of different click-type reactions including thiol-maleimide, copper-catalyzed-azide-alkyne-cycloaddition (CuAAC) and strain-promoted-azide-alkyne-cycloaddition (SPAAC)...
October 12, 2016: Bioconjugate Chemistry
Kay M Tomashek, Aidsa Rivera, Brenda Torres-Velasquez, Elizabeth A Hunsperger, Jorge L Munoz-Jordan, Tyler M Sharp, Irma Rivera, Dario Sanabria, Dianna M Blau, Renee Galloway, Jose Torres, Rosa Rodriguez, Javier Serrano, Carlos Chávez, Francisco Dávila, Janice Perez-Padilla, Esther M Ellis, Gladys Caballero, Laura Wright, Sherif R Zaki, Carmen Deseda, Edda Rodriguez, Harold S Margolis
BACKGROUND: Dengue is a leading cause of morbidity throughout the tropics; however, accurate population-based estimates of mortality rates are not available. METHODS/PRINCIPAL FINDINGS: We established the Enhanced Fatal Acute Febrile Illness Surveillance System (EFASS) to estimate dengue mortality rates in Puerto Rico. Healthcare professionals submitted serum and tissue specimens from patients who died from a dengue-like acute febrile illness, and death certificates were reviewed to identify additional cases...
October 2016: PLoS Neglected Tropical Diseases
Bin-Yan Wu, Jian-Ren Ye, Lin Huang, Ling-Min He, De-Wei Li
Burkholderia pyrrocinia strain JK-SH007 isolated from poplar stems plays a highly significant role in the growth promotion and the biocontrol of poplar canker during colonization in poplar. In this research, the ideal reference gene was filtered and determined for the transcript normalization. Additionally, the expression of pyrG under all four conditions was relatively stable in B. pyrrocinia JK-SH007.
October 7, 2016: Journal of Microbiological Methods
Rodrigo S Raposo, M Catarina M D de Almeida, M da Conceição M A de Oliveira, M Manuela da Fonseca, M Teresa Cesário
Efficient production of poly-3-hydroxybutyrate (P(3HB)) based on glucose-xylose mixtures simulating different types of lignocellulosic hydrolysate (LCH) was addressed using Burkholderia sacchari, a wild strain capable of metabolizing both sugars and producing P(3HB). Carbon catabolite repression was avoided by maintaining glucose concentration below 10g/L. Xylose concentrations above 30g/L were inhibitory for growth and production. In fed-batch cultivations, pulse size and feed addition rate were controlled in order to reach high productivities and efficient sugar consumptions...
October 5, 2016: New Biotechnology
Yuichi Katanami, Satoshi Kutsuna, Atsuko Horino, Takehiro Hashimoto, Yoshikazu Mutoh, Kei Yamamoto, Nozomi Takeshita, Kayoko Hayakawa, Shuzo Kanagawa, Yasuyuki Kato, Norio Ohmagari
Melioidosis, an infectious disease with high mortality, caused by Burkholderia pseudomallei, is endemic in southeast Asia and northern Australia. In Indonesia, autochthonous cases have been rarely reported, with most cases being sporadic and occurring in travelers. Herein, we report a fatal case of neurological melioidosis in a traveler from Indonesia presenting with septic shock.
October 5, 2016: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
Nazanin Seyed Khoei, Silvia Lampis, Emanuele Zonaro, Kim Yrjälä, Paolo Bernardi, Giovanni Vallini
Microorganisms capable of transforming toxic selenium oxyanions into non-toxic elemental selenium (Se°) may be considered as biocatalysts for the production of selenium nanoparticles (SeNPs), eventually exploitable in different biotechnological applications. Two Burkholderia fungorum strains (B. fungorum DBT1 and B. fungorum 95) were monitored during their growth for both capacity and efficiency of selenite (SeO3(2-)) reduction and elemental selenium formation. Both strains are environmental isolates in origin: B...
October 4, 2016: New Biotechnology
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