keyword
MENU ▼
Read by QxMD icon Read
search

burkholderia

keyword
https://www.readbyqxmd.com/read/29234312/parallelized-aerobic-single-carbon-source-enrichments-from-different-natural-environments-contain-divergent-microbial-communities
#1
Theodore M Flynn, Jason C Koval, Stephanie M Greenwald, Sarah M Owens, Kenneth M Kemner, Dionysios A Antonopoulos
Microbial communities that inhabit environments such as soil can contain thousands of distinct taxa, yet little is known about how this diversity is maintained in response to environmental perturbations such as changes in the availability of carbon. By utilizing aerobic substrate arrays to examine the effect of carbon amendment on microbial communities taken from six distinct environments (soil from a temperate prairie and forest, tropical forest soil, subalpine forest soil, and surface water and soil from a palustrine emergent wetland), we examined how carbon amendment and inoculum source shape the composition of the community in each enrichment...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29233899/detection-of-cytosolic-shigella-flexneri-via-a-c-terminal-triple-arginine-motif-of-gbp1-inhibits-actin-based-motility
#2
Anthony S Piro, Dulcemaria Hernandez, Sarah Luoma, Eric M Feeley, Ryan Finethy, Azeb Yirga, Eva M Frickel, Cammie F Lesser, Jörn Coers
Dynamin-like guanylate binding proteins (GBPs) are gamma interferon (IFN-γ)-inducible host defense proteins that can associate with cytosol-invading bacterial pathogens. Mouse GBPs promote the lytic destruction of targeted bacteria in the host cell cytosol, but the antimicrobial function of human GBPs and the mechanism by which these proteins associate with cytosolic bacteria are poorly understood. Here, we demonstrate that human GBP1 is unique among the seven human GBP paralogs in its ability to associate with at least two cytosolic Gram-negative bacteria, Burkholderia thailandensis and Shigella flexneri Rough lipopolysaccharide (LPS) mutants of S...
December 12, 2017: MBio
https://www.readbyqxmd.com/read/29233255/burkholderia-stabilis-outbreak-associated-with-contaminated-commercially-available-washing-gloves-switzerland-may-2015-to-august-2016
#3
Rami Sommerstein, Urs Führer, Elia Lo Priore, Carlo Casanova, Dominik M Meinel, Helena Mb Seth-Smith, Andreas Kronenberg, On Behalf Of Anresis, Daniel Koch, Laurence Senn, Andreas F Widmer, Adrian Egli, Jonas Marschall, On Behalf Of Swissnoso
We describe an outbreak of Burkholderia stabilis associated with contaminated washing gloves, a commercially available Class I medical device. Triggered by an increase in Burkholderia cepacia complex (BCC) bacteremias and the detection of BCC in unopened packages of washing gloves, an ad hoc national outbreak committee comprising representatives of a public health organisation, a regulatory agency, and an expert association convened and commissioned an outbreak investigation. The investigation included retrospective case finding across Switzerland and whole genome sequencing (WGS) of isolates from cases and gloves...
December 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/29232837/a-burkholderia-pseudomallei-outer-membrane-vesicle-vaccine-provides-cross-protection-against-inhalational-glanders-in-mice-and-non-human-primates
#4
Sarah M Baker, Christopher J H Davitt, Natalya Motyka, Nicole L Kikendall, Kasi Russell-Lodrigue, Chad J Roy, Lisa A Morici
Burkholderia mallei is a Gram-negative, non-motile, facultative intracellular bacillus and the causative agent of glanders, a highly contagious zoonotic disease. B. mallei is naturally resistant to multiple antibiotics and there is concern for its potential use as a bioweapon, making the development of a vaccine against B. mallei of critical importance. We have previously demonstrated that immunization with multivalent outer membrane vesicles (OMV) derived from B. pseudomallei provide significant protection against pneumonic melioidosis...
December 9, 2017: Vaccines
https://www.readbyqxmd.com/read/29228248/comparison-of-the-intestinal-mucosal-microbiota-in-dogs-diagnosed-with-idiopathic-inflammatory-bowel-disease-and-dogs-with-food-responsive-diarrhea-before-and-after-treatment
#5
K Kalenyak, A Isaiah, R M Heilmann, J S Suchodolski, I A Burgener
We report the first study to evaluate the intestinal mucosal microbiota of dogs with inflammatory bowel disease (IBD) and dogs with food-responsive diarrhea (FRD) before and after treatment. It was hypothesized that differences in the microbial composition exist between both disease groups and within groups pre- vs. post-treatment. Duodenal and colonic biopsies were obtained endoscopically from 24 dogs (15 FRD, 9 IBD) before and after treatment. The intestinal microbiota was evaluated by Illumina sequencing of the bacterial 16S rRNA gene...
December 6, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29228063/what-matters-in-chronic-burkholderia-cenocepacia-infection-in-cystic-fibrosis-insights-from-comparative-genomics
#6
Jaroslav Nunvar, Vaclav Capek, Karel Fiser, Libor Fila, Pavel Drevinek
Burkholderia cenocepacia causes severe pulmonary infections in cystic fibrosis (CF) patients. Since the bacterium is virtually untreatable by antibiotics, chronic infections persist for years and might develop into fatal septic pneumonia (cepacia syndrome, CS). To devise new strategies to combat chronic B. cenocepacia infections, it is essential to obtain comprehensive knowledge about their pathogenesis. We conducted a comparative genomic analysis of 32 Czech isolates of epidemic clone B. cenocepacia ST32 isolated from various stages of chronic infection in 8 CF patients...
December 11, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29215024/identification-of-srna-mediated-responses-to-nutrient-depletion-in-burkholderia-pseudomallei
#7
Hirzahida Mohd-Padil, Nadzirah Damiri, Suhaila Sulaiman, Shiao-Fei Chai, Sheila Nathan, Mohd Firdaus-Raih
The Burkholderia genus includes many species that are known to survive in diverse environmental conditions including low nutrient environments. One species, Burkholderia pseudomallei is a versatile pathogen that can survive in a wide range of hosts and environmental conditions. In this study, we investigated how a nutrient depleted growth environment evokes sRNA mediated responses by B. pseudomallei. Computationally predicted B. pseudomallei D286 sRNAs were mapped to RNA-sequencing data for cultures grown under two conditions: (1) BHIB as a nutrient rich media reference environment and (2) M9 media as a nutrient depleted stress environment...
December 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29214486/metabolism-mediated-induction-of-zinc-tolerance-in-brassica-rapa-by-burkholderia-cepacia-cs2-1
#8
Sang-Mo Kang, Raheem Shahzad, Saqib Bilal, Abdul Latif Khan, Young-Hyun You, Won-Hee Lee, Hee-La Ryu, Ko-Eun Lee, In-Jung Lee
Brassica rapa (Chinese cabbage) is an essential component of traditional Korean food. However, the crop is often subject to zinc (Zn+) toxicity from contaminated irrigation water, which, as a result, compromises plant growth and production, as well as the health of human consumers. The present study investigated the bioaccumulation of Zn+ by Burkholderia cepacia CS2-1 and its effect on the heavy metal tolerance of Chinese cabbage. Strain CS2-1 was identified and characterized on the basis of 16S rRNA sequences and phylogenetic analysis...
December 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29212292/construction-of-luminescence-and-fluorescence-tagged-burkholderia-pseudomallei-for-pathogen-tracking-in-a-mouse-model
#9
Yong-Woo Shin, Deok Bum Park, Myung-Min Choi, Jeong-Hoon Chun, Baik-Lin Seong, Gi-Eun Rhie
Molecular imaging is a powerful method for tracking various infectious disease causing pathogens in host organisms. Currently, a dual molecular imaging method which can provide temporal and spatial information in infected hosts at organism, organ, tissue and cellular levels simultaneously has not been reported for Burkholderia pseudomallei, a high-risk pathogen that causes melioidosis. In this study, we have established an experiment method that provides spatio-temporal information in infected hosts using luminescent and fluorescent dual-labeled B...
December 8, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29209289/co-existence-of-rhizobia-and-diverse-non-rhizobial-bacteria-in-the-rhizosphere-and-nodules-of-dalbergia-odorifera-seedlings-inoculated-with-bradyrhizobium-elkanii-rhizobium-multihospitium-like-and-burkholderia-pyrrocinia-like-strains
#10
Junkun Lu, Fucheng Yang, Shengkun Wang, Haibin Ma, Junfeng Liang, Yinglong Chen
Rhizobia induce root nodules and fix atmospheric N2 for most legume species in exchange for carbon. However, the diverse endophytic non-rhizobial bacteria in legume nodules that co-exist with rhizobia are often ignored because they are difficult to cultivate using routine cultivation approaches. To enhance our understanding of the incidence and diversity of legume-bacteria associations, a high-throughput sequencing analysis of bacterial 16S rRNA genes was used to examine the bacterial community in the rhizospheres and root nodules of Dalbergia odorifera seedlings that were uninoculated or inoculated with Bradyrhizobium elkanii H255, Rhizobium multihospitium-like HT221, or Burkholderia pyrrocinia-like H022238, in two growth media (nitrogen [N]-supplied soil or N-omitted potting mix)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29208745/the-complex-quorum-sensing-circuitry-of-burkholderia-thailandensis-is-both-hierarchically-and-homeostatically-organized
#11
Servane Le Guillouzer, Marie-Christine Groleau, Eric Déziel
The genome of the bacterium Burkholderia thailandensis encodes three complete LuxI/LuxR-type quorum sensing (QS) systems: BtaI1/BtaR1 (QS-1), BtaI2/BtaR2 (QS-2), and BtaI3/BtaR3 (QS-3). The LuxR-type transcriptional regulators BtaR1, BtaR2, and BtaR3 modulate the expression of target genes in association with various N-acyl-l-homoserine lactones (AHLs) as signaling molecules produced by the LuxI-type synthases BtaI1, BtaI2, and BtaI3. We have systematically dissected the complex QS circuitry of B. thailandensis strain E264...
December 5, 2017: MBio
https://www.readbyqxmd.com/read/29208140/suspected-cases-of-intracontinental-burkholderia-pseudomallei-sequence-type-homoplasy-resolved-using-whole-genome-sequencing
#12
Ammar Aziz, Derek S Sarovich, Tegan M Harris, Mirjam Kaestli, Evan McRobb, Mark Mayo, Bart J Currie, Erin P Price
Burkholderia pseudomallei is a Gram-negative environmental bacterium that causes melioidosis, a disease of high mortality in humans and animals. Multilocus sequence typing (MLST) is a popular and portable genotyping method that has been used extensively to characterise the genetic diversity of B. pseudomallei populations. MLST has been central to our understanding of the underlying phylogeographical signal present in the B. pseudomallei genome, revealing distinct populations on both the intra- and the inter-continental level...
November 2017: Microbial Genomics
https://www.readbyqxmd.com/read/29208119/comparative-analysis-of-the-burkholderia-cenocepacia-k56-2-essential-genome-reveals-cell-envelope-functions-that-are-uniquely-required-for-survival-in-species-of-the-genus-burkholderia
#13
April S Gislason, Keith Turner, Mike Domaratzki, Silvia T Cardona
Burkholderia cenocepacia K56-2 belongs to the Burkholderia cepacia complex, a group of Gram-negative opportunistic pathogens that have large and dynamic genomes. In this work, we identified the essential genome of B. cenocepacia K56-2 using high-density transposon mutagenesis and insertion site sequencing (Tn-seq circle). We constructed a library of one million transposon mutants and identified the transposon insertions at an average of one insertion per 27 bp. The probability of gene essentiality was determined by comparing of the insertion density per gene with the variance of neutral datasets generated by Monte Carlo simulations...
November 2017: Microbial Genomics
https://www.readbyqxmd.com/read/29204272/novel-antibiotic-combinations-proposed-for-treatment-of-burkholderia-cepacia-complex-infections
#14
Omar M El-Halfawy, Marwa M Naguib, Miguel A Valvano
Effective strategies to manage Burkholderia cepacia complex (Bcc) infections in cystic fibrosis (CF) patients are lacking. We tested combinations of clinically available antibiotics and show that moxifloxacin-ceftazidime could inhibit 16 Bcc clinical isolates at physiologically achievable concentrations. Adding low dose of colistin improved the efficacy of the combo, especially at conditions mimicking CF respiratory secretions.
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29201605/culture-free-detection-of-crop-pathogens-at-the-single-cell-level-by-micro-raman-spectroscopy
#15
Qinhua Gan, Xuetao Wang, Yun Wang, Zhenyu Xie, Yang Tian, Yandu Lu
The rapid and sensitive identification of invasive plant pathogens has important applications in biotechnology, plant quarantine, and food security. Current methods are far too time-consuming and need a pre-enrichment period ranging from hours to days. Here, a micro-Raman spectroscopy-based bioassay for culture-free pathogen quarantine inspection at the single cell level within 40 min is presented. The application of this approach can readily and specifically detect plant pathogens Burkholderia gladioli pv...
November 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/29199856/antimicrobial-activity-of-six-essential-oils-against-burkholderia-cepacia-complex-insights-into-mechanism-s-of-action
#16
Elena Perrin, Valentina Maggini, Isabel Maida, Eugenia Gallo, Katia Lombardo, Maria Pia Madarena, Silvia Buroni, Viola Camilla Scoffone, Fabio Firenzuoli, Alessio Mengoni, Renato Fani
AIM: To investigate the activity and mechanisms of action of six essential oils (EOs) against Burkholderia cepacia complex, opportunistic human pathogens highly resistant to antibiotics. MATERIALS & METHODS: Minimal inhibitory concentration of EOs alone, plus antibiotics or efflux pump inhibitors was determined. RESULTS: Origanum vulgare, Thymus vulgaris and Eugenia caryophyllata EOs resulted to be more active than the other EOs. EOs did not enhance antibiotic activity against the model strain B...
December 4, 2017: Future Microbiology
https://www.readbyqxmd.com/read/29194875/heterologous-expression-biosynthetic-studies-and-ecological-function-of-the-selective-gq-signaling-inhibitor-fr900359
#17
Max Crüsemann, Raphael Reher, Isabella Schamari, Alexander O Brachmann, Tsubasa Ohbayashi, Markus Kuschak, Davide Malfacini, Alexander Seidinger, Marta Pinto-Carbó, René Richarz, Tatjana Reuter, Stefan Kehraus, Asis Hallab, Misty Attwood, Helgi B Schiöth, Peter Mergaert, Yoshitomo Kikuchi, Till F Schäberle, Evi Kostenis, Daniela Wenzel, Christa E Müller, Jörn Piel, Aurélien Carlier, Leo Eberl, Gabriele Maria Koenig
The cyclic depsipeptide FR900359 (FR), isolated from the tropical plant Ardisia crenata, displays a strong and selective inhibition of Gq proteins, making it an indispensable pharmacological tool to study Gq-related processes, as well as a promising drug candidate. Gq inhibition is a novel mode of action for defense chemicals and crucial for the ecological function of FR, as shown by in vivo experiments on mice, affinity to insect Gq proteins and insect toxicity studies. The uncultured endosymbiont of A. crenata, 'Candidatus Burkholderia crenata' was sequenced, revealing the FR nonribosomal peptide synthetase (frs) gene cluster...
December 1, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29193830/effects-of-cosmetics-on-the-skin-microbiome-of-facial-cheeks-with-different-hydration-levels
#18
Hyo Jung Lee, Sang Eun Jeong, Soyoun Lee, Sungwoo Kim, Hyuntak Han, Che Ok Jeon
Basic cosmetics was used by volunteers belonging to high (HHG) and low (LHG) hydration groups for 4 weeks, and bacterial communities and biophysical parameters in facial skin were analyzed. Hydration level increases and transepidermal water loss and roughness decreases were observed in both groups after cosmetic use. Bacterial diversity was greater in LHG than HHG, and increased after cosmetic use in both groups. Bray-Curtis dissimilarities that were higher in LHG than HHG increased in HHG after cosmetic use, whereas they decreased in LHG...
November 29, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/29188170/the-role-of-climate-in-the-epidemiology-of-melioidosis
#19
REVIEW
Adam J Merritt, Timothy J J Inglis
Purpose of Review: Melioidosis epidemiology is susceptible to climate change through direct and indirect effects on human encounter with the causative agent, Burkholderia pseudomallei. This review describes the current depth of knowledge and recent advances in the understanding of this relationship and applies it to observations of melioidosis in Western Australia. Recent Findings: High maximum rainfall and dense cloud cover have been shown to predict environmental presence of B...
2017: Current Tropical Medicine Reports
https://www.readbyqxmd.com/read/29187254/acute-disseminated-melioidosis-giving-rise-to-pneumonia-and-renal-abscesses-complicated-with-thrombotic-thrombocytopenic-purpura-in-a-post-partum-woman-a-case-report
#20
Piyumi Sachindra Alwis Wijewickrama, Rohini Weerakoon
BACKGROUND: Melioidosis is an established endemic infection in Sri Lanka, caused by Burkholderia pseudomallei, a gram negative bacterium distributed in saprophytes in soil and surface water. Main mode of transmission is via percutaneous inoculation. Pneumonia is the most common presentation in acute disease. CASE PRESENTATION: We report a 33 year old previously healthy Sinhalese female with an occupational exposure to surface water in paddy fields, who was on postpartum day 6 following an uncomplicated pregnancy and delivery via an elective caesarian section...
November 29, 2017: BMC Research Notes
keyword
keyword
11357
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"