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https://www.readbyqxmd.com/read/29043376/enhanced-rhamnolipid-production-in-burkholderia-thailandensis-transposon-knockout-strains-deficient-in-polyhydroxyalkanoate-pha-synthesis
#1
Scott J Funston, Konstantina Tsaousi, Thomas J Smyth, Matthew S Twigg, Roger Marchant, Ibrahim M Banat
Microbially produced rhamnolipids have significant commercial potential; however, the main bacterial producer, Pseudomonas aeruginosa, is an opportunistic human pathogen, which limits biotechnological exploitation. The non-pathogenic species Burkholderia thailandensis produces rhamnolipids; however, yield is relatively low. The aim of this study was to determine whether rhamnolipid production could be increased in Burkholderia thailandensis through mutation of genes responsible for the synthesis of the storage material polyhydroxyalkanoate (PHA), thereby increasing cellular resources for the production of rhamnolipids...
October 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29033316/synergy-and-target-promiscuity-drive-structural-divergence-in-bacterial-alkylquinolone-biosynthesis
#2
Yihan Wu, Mohammad R Seyedsayamdost
Microbial natural products are genetically encoded by dedicated biosynthetic gene clusters (BGCs). A given BGC usually produces a family of related compounds that share a core but contain variable substituents. Though common, the reasons underlying this divergent biosynthesis are in general unknown. Herein, we have addressed this issue using the hydroxyalkylquinoline (HAQ) family of natural products synthesized by Burkholderia thailandensis. Investigations into the detailed functions of two analogs show that they act synergistically in inhibiting bacterial growth...
September 28, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/29032643/effects-of-extended-storage-of-chlorhexidine-gluconate-and-benzalkonium-chloride-solutions-on-the-viability-of-burkholderia-cenocepacia
#3
Youngbeom Ahn, Jeong Myeong Kim, Yong-Jin Lee, John J LiPuma, David Hussong, Bernard S Marasa, Carl E Cerniglia
Chlorhexidine gluconate (CHX) and benzalkonium chloride (BZK) formulations are frequently used as antiseptics in healthcare and consumer products. Burkholderia cepacia complex (BCC) contamination of pharmaceutical products could be due to the use of contaminated water in the manufacturing process, over-diluted antiseptic solutions in the product, and the use of outdated products, which in turn, reduces the antimicrobial activity of CHX and BZK. To establish a "safe use" period following opening containers of CHX and BZK, we measured the antimicrobial effects of CHX (2 ~ 10 µg/ml) and BZK (10 ~ 50 µg/ml) at sub-lethal concentrations on six strains of Burkholderia cenocepacia using chemical and microbiological assays...
October 14, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29030528/real-time-pcr-detection-of-burkholderia-cepacia-in-pharmaceutical-products-contaminated-with-low-levels-of-bacterial-contamination
#4
Luis Jimenez, Theranda Jashari, Jenifer Vasquez, Stephanie Zapata, Joy Bochis, Margarita Kulko, Victoria Ellman, Matthew Gardner, Tina Choe
A Real-Time (RT) PCR assay was developed to detect Burkholderia cepacia in pharmaceutical products contaminated with low levels of bacterial contamination. Different pharmaceutical suspensions were artificially contaminated with B. cepacia, Escherichia coli, Staphylococcus aureus, and Bacillus megaterium. After a 24-hour incubation in TSB with Tween 20, samples were streaked on mannitol salt, phenyl ethyl alcohol, eosin methylene blue, MacConkey, and pseudomonas isolation agar. Microbial DNA was extracted from each sample by using a Tris-EDTA, proteinase K, Tween 20 buffer...
October 12, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/29026657/whole-genome-sequencing-to-investigate-a-non-clonal-melioidosis-cluster-on-a-remote-australian-island
#5
Derek S Sarovich, Stephanie N J Chapple, Erin P Price, Mark Mayo, Matthew T G Holden, Sharon J Peacock, Bart J Currie
Melioidosis is a tropical disease caused by the bacterium Burkholderia pseudomallei. Outbreaks are uncommon and can generally be attributed to a single point source and strain. We used whole-genome sequencing to analyse B. pseudomallei isolates collected from an historical 2-year long case cluster that occurred in a remote northern Australian indigenous island community, where infections were previously linked to a contaminated communal water supply. We analysed the genome-wide relatedness of the two most common multilocus sequence types (STs) involved in the outbreak, STs 125 and 126...
August 2017: Microbial Genomics
https://www.readbyqxmd.com/read/29025950/high-quality-draft-genome-sequence-of-burkholderia-contaminans-ch-1-a-gram-negative-bacterium-that-metabolizes-2-azahypoxanthine-a-plant-growth-regulating-compound
#6
Jae-Hoon Choi, Hikaru Sugiura, Ryota Moriuchi, Hirokazu Kawagishi, Hideo Dohra
Burkholderia contaminans strain CH-1 converts 2-azahypoxnathine to 2-aza-8-oxohypoxanthine, plant growth-regulating compounds, by oxidation. We report here the high-quality draft genome sequence of B. contaminans CH-1. The genome contains 8,065 protein-coding sequences, including several genes possibly involved in metabolizing 2-azahypoxanthine.
October 12, 2017: Genome Announcements
https://www.readbyqxmd.com/read/29024844/structural-characterisation-of-the-capsular-polysaccharide-expressed-by-burkholderia-thailandensis-strain-e555-wbii-pknock-kmr-and-assessment-of-the-significance-of-the-2-o-acetyl-group-in-immune-protection
#7
Marc Bayliss, Matthew I Donaldson, Sergey A Nepogodiev, Giulia Pergolizzi, Andrew E Scott, Nicholas J Harmer, Robert A Field, Joann L Prior
Burkholderia pseudomallei and its close relative B. mallei are human pathogens that are classified as Tier 1 bio-threat agents. Both organisms have previously been shown to constitutively produce a capsular polysaccharide (CPS) that is both a virulence determinant and protective antigen. Extraction and purification of CPS for use as a potential vaccine candidate requires containment level 3 laboratories which is expensive and time-consuming. B. thailandensis strain E555 is closely related to B. pseudomallei and B...
September 21, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/29022243/pyrosequencing-analysis-of-source-water-switch-and-sulfate-induced-bacterial-community-transformation-in-simulated-drinking-water-distribution-pipes
#8
Fan Yang, Baoyou Shi, Weiyu Zhang, Jing Cui, Jianbo Guo, Dongsheng Wang, Nan Wu, Xinyuan Liu
Inter-basin water transfer and source water switching will be increasingly launched due to significant population increase and the shortage of the local water resources in cities around the world. Source water switch may cause physiochemical and microbiological de-stabilization of pipe material, biofilms, and loose deposits in drinking water distribution system (DWDS). Great sulfate alteration during source water switch had been deemed as the main cause of a red water case that occurred in a northern China city...
October 11, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29022160/a-catalyst-from-burkholderia-cenocepacia-for-efficient-anti-prelog-s-bioreduction-of-3-5-bis-trifluoromethyl-acetophenone
#9
Songzhu Yu, Hongxia Li, Yan Lu, Guojun Zheng
(R)-3, 5-Bis(trifluoromethyl)phenyl ethanol is a key chiral intermediate for the synthesis of aprepitant. Through a genome mining approach, an NADPH-dependent short-chain dehydrogenases derived from Burkholderia cenocepacia (Bc-SDR) was discovered with excellent anti-Prelog's stereoselectivity of reducing 3, 5-bis(trifluoromethyl) acetophenone. The enzyme with 247 amino acids was successfully expressed in Escherichia coli and the molecular weight was about 26 kDa. Optimization of reaction conditions showed that the optimum temperature and pH of the enzyme was 25 °C and pH 7...
October 12, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29021156/safety-and-accuracy-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-to-identify-highly-pathogenic-organisms
#10
James T Rudrik, Marty K Soehnlen, Michael J Perry, Maureen Sullivan, Wanda Reiter-Kintz, Philip A Lee, Denise Pettit, Anthony Tran, Erin Swaney
Matrix-assisted laser desorption ionization -- time of flight mass spectrometry (MALDI-TOF MS) sample preparation methods including the direct, on-plate formic acid, and ethanol/formic acid tube extraction were evaluated for their ability to render highly pathogenic organisms non-viable and safe for handling in a Biosafety Level-2 laboratory. Of these, the tube extraction procedure was the most successful, with none of the tested strains surviving this sample preparation method. Tube extracts from several agents of bioterrorism and their near neighbors were analyzed in an eight laboratory study to examine the utility of the Bruker Biotyper and Vitek MS MALDI-TOF MS systems and their IVD, research use only, and Security-Relevant databases, as applicable, to accurately identify these agents...
October 11, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29017247/a-17-year-nationwide-study-of-burkholderia-cepacia-complex-bloodstream-infections-among-patients-in-the-united-states-veterans-health-administration
#11
Nadim G El Chakhtoura, Elie Saade, Brigid M Wilson, Federico Perez, Krisztina M Papp-Wallace, Robert A Bonomo
Background: Burkholderia cepacia complex (Bcc) are a group of multidrug-resistant gram-negative bacteria rarely reported in patients without cystic fibrosis (CF) or immunocompromising conditions. We investigated Bcc bloodstream infections (BSIs) in a cohort of non-CF patients from the US Veterans Health Administration (VHA). Methods: Using VHA databases, we identified patients with Bcc BSI at facilities nationwide from 1999 through 2015. We ascertained clinical characteristics, treatments, and outcomes and identified factors associated with 30-day mortality in logistic regression analysis...
October 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29016340/presence-of-burkholderia-pseudomallei-in-soil-and-paddy-rice-water-in-a-rice-field-in-northeast-thailand-but-not-in-air-and-rainwater
#12
Catherine E L Ong, Gumphol Wongsuvan, Janet S W Chew, Tan Yian Kim, Low Hwee Teng, Premjit Amornchai, Vanaporn Wuthiekanun, Nicholas P J Day, Sharon J Peacock, Tan Yoke Cheng, Eric P H Yap, Direk Limmathurotsakul
Environmental Burkholderia pseudomallei has been postulated to be aerosolized during ploughing and heavy rain, and could result in inhalational melioidosis. Here, we determined the presence of B. pseudomallei in soil, paddy field water (PFW), air, and rainwater samples in a single rice paddy field in Ubon Ratchathani, northeast Thailand. In 2012, we collected 100 soil samples during the dry season, 10 PFW samples during the monsoon season, 77 air samples during ploughing (N = 31) and heavy rains (N = 46), and 60 rainwater samples during 12 rain events...
October 2, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29016319/clinical-epidemiology-of-septic-arthritis-caused-by-burkholderia-pseudomallei-and-other-bacterial-pathogens-in-northeast-thailand
#13
Prapit Teparrukkul, Jiraphorn Nilsakul, Susanna Dunachie, Direk Limmathurotsakul
Septic arthritis is a medical emergency, and if not treated appropriately, it can be associated with high morbidity and mortality. Melioidosis, a serious infectious disease caused by the Gram-negative bacillus Burkholderia pseudomallei, is highly endemic in South and Southeast Asia and northern Australia. We reviewed the medical charts of adult patients admitted with bacterial septic arthritis at Sunpasitthiprasong Hospital, Ubon Ratchathani, northeast Thailand from January 2012 to December 2014. Bacterial septic arthritis was defined as one or more hot swollen joints with isolation of a pathogenic organism from an affected joint or from blood...
October 2, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28994855/melioidos-%C3%A2-en-viktig-diagnos-vid-sv%C3%A3-r-sjukdom-efter-utlandsresa
#14
Åsa Gylfe, Sara Cajander, Tara Wahab, Martin Angelin
Melioidosis, an important diagnosis in the severely ill traveler Melioidosis is a common tropical infection in Southeast Asia and is caused by the highly pathogenic soil bacterium Burkholderia pseudomallei. Diagnosis and treatment is often challenging due to variations in clinical presentation, limited antibiotic susceptibility and high risk of recurring infection. In this report, three cases with different clinical presentations are described.
October 9, 2017: Läkartidningen
https://www.readbyqxmd.com/read/28988383/diversity-of-free-living-amoebae-in-soils-and-their-associated-human-opportunistic-bacteria
#15
Elodie Denet, Bénédicte Coupat-Goutaland, Sylvie Nazaret, Michel Pélandakis, Sabine Favre-Bonté
Free-living amoebae (FLA) are ubiquitous protozoa found worldwide in the environment. They feed by phagocytosis on various microorganisms. However, some bacteria, i.e., amoebae-resistant bacteria (ARB) or bacterial endocytobionts, can resist phagocytosis and even multiply inside FLA. This study investigated the diversity of culturable FLA in various soils from agricultural and mining sites and their bacterial endocytobionts. FLA were cultured on non-nutrient agar with alive Escherichia coli and identified by PCR and sequencing...
October 7, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28986923/crystal-structure-of-d-glycero-%C3%AE-d-manno-heptose-1-phosphate-adenylyltransferase-from-burkholderia-pseudomallei
#16
Jimin Park, Hyojin Kim, Suwon Kim, Daeun Lee, Mi-Sun Kim, Dong Hae Shin
The crystal structure of HldC from B. pseudomallei (BpHldC), the fourth enzyme of the heptose biosynthesis pathway, has been determined. BpHldC converts ATP and d-glycero-β-d-manno-heptose-1-phosphate into ADP-d-glycero-β -d-manno-heptose and pyrophosphate. The crystal structure of BpHldC belongs to the nucleotidyltransferase α/β phosphodiesterase superfamily sharing a common Rossmann-like α/β fold with a conserved T/HXGH sequence motif. The invariant catalytic key residues of BpHldC indicate that the core catalytic mechanism of BpHldC may be similar to that of other closest homologues...
October 7, 2017: Proteins
https://www.readbyqxmd.com/read/28982193/cysteamine-mediated-clearance-of-antibiotic-resistant-pathogens-in-human-cystic-fibrosis-macrophages
#17
Chandra L Shrestha, Kaivon D Assani, Hannah Rinehardt, Florentina Albastroiu, Shuzhong Zhang, Richard Shell, Amal O Amer, Larry S Schlesinger, Benjamin T Kopp
Members of the Burkholderia cepacia complex are virulent, multi-drug resistant pathogens that survive and replicate intracellularly in patients with cystic fibrosis (CF). We have discovered that B. cenocepacia cannot be cleared from CF macrophages due to defective autophagy, causing continued systemic inflammation and infection. Defective autophagy in CF is mediated through constitutive reactive oxygen species (ROS) activation of transglutaminase-2 (TG2), which causes the sequestration (accumulation) of essential autophagy initiating proteins...
2017: PloS One
https://www.readbyqxmd.com/read/28978597/melioidosis-presenting-as-septicaemia-and-facial-nerve-palsy
#18
Tessa Antony, Swathy Moorthy, Anbumani Narayanaswamy, Preetam Arthur
A 43-year-old farmer presented with acute onset pneumonia, septicaemia and peripheral facial nerve palsy (left side). Burkholderia pseudomallei was isolated from the blood culture of the patient. The patient was successfully treated with intravenous meropenem and oral cotrimoxazole for 2 weeks followed by maintenance therapy with cotrimoxazole. The case is reported to increase awareness among the clinicians and microbiologists regarding melioidosis.
October 4, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28973030/cholesterol-and-host-cell-surface-proteins-contribute-to-cell-cell-fusion-induced-by-the-burkholderia-type-vi-secretion-system-5
#19
Liam Whiteley, Maria Haug, Kristina Klein, Matthias Willmann, Erwin Bohn, Salvatore Chiantia, Sandra Schwarz
Following escape into the cytoplasm of host cells, Burkholderia pseudomallei and the related species Burkholderia thailandensis employ the type VI secretion system 5 (T6SS-5) to induce plasma membrane fusion with an adjacent host cell. This process leads to the formation of multinucleated giant cells and facilitates bacterial access to an uninfected host cell in a direct manner. Despite its importance in virulence, the mechanism of the T6SS-5 and the role of host cell factors in cell-cell fusion remain elusive...
2017: PloS One
https://www.readbyqxmd.com/read/28972189/the-lipopolysaccharide-core-oligosaccharide-of-burkholderia-plays-a-critical-role-in-maintaining-a-proper-gut-symbiosis-with-the-bean-bug-riptortus-pedestris
#20
Jiyeun Kate Kim, Ho Am Jang, Min Seon Kim, Jae Hyun Cho, Jum Beom Lee, Flaviana Di Lorenzo, Luisa Sturiale, Alba Silipo, Antonio Molinaro, Bok Luel Lee
Lipopolysaccharide, the outer cell wall component of Gram-negative bacteria, has been shown to be important for symbiotic associations. We recently reported that the lipopolysaccharide O-antigen of Burkholderia enhances the initial colonization of the midgut of the bean bug, Riptortus pedestris. However, the midgut-colonizing Burkholderia symbionts lack the O-antigen, but display the core oligosaccharide on the cell surface. In this study, we investigated the role of the core oligosaccharide, which directly interacts with the host midgut, in the Riptortus-Burkholderia symbiosis...
September 25, 2017: Journal of Biological Chemistry
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