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Spermatogenesis, lc-ms

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https://www.readbyqxmd.com/read/27545216/proteomic-characterization-of-histone-variants-in-the-mouse-testis-by-mass-spectrometry-based-top-down-analysis
#1
Ho-Geun Kwak, Naoshi Dohmae
Various histones, including testis-specific histones, exist during spermatogenesis and some of them have been reported to play a key role in chromatin remodeling. Mass spectrometry (MS)-based characterization has become the important step to understand histone structures. Although individual histones or partial histone variant groups have been characterized, the comprehensive analysis of histone variants has not yet been conducted in the mouse testis. Here, we present the comprehensive separation and characterization of histone variants from mouse testes by a top-down approach using MS...
November 15, 2016: Bioscience Trends
https://www.readbyqxmd.com/read/27330565/comprehensive-analysis-of-histone-post-translational-modifications-in-mouse-and-human-male-germ-cells
#2
Lacey J Luense, Xiaoshi Wang, Samantha B Schon, Angela H Weller, Enrique Lin Shiao, Jessica M Bryant, Marisa S Bartolomei, Christos Coutifaris, Benjamin A Garcia, Shelley L Berger
BACKGROUND: During the process of spermatogenesis, male germ cells undergo dramatic chromatin reorganization, whereby most histones are replaced by protamines, as part of the pathway to compact the genome into the small nuclear volume of the sperm head. Remarkably, approximately 90 % (human) to 95 % (mouse) of histones are evicted during the process. An intriguing hypothesis is that post-translational modifications (PTMs) decorating histones play a critical role in epigenetic regulation of spermatogenesis and embryonic development following fertilization...
2016: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/27173832/spermatogenesis-associated-proteins-at-different-developmental-stages-of-buffalo-testicular-seminiferous-tubules-identified-by-comparative-proteomic-analysis
#3
Yu-Lin Huang, Qiang Fu, Hong Pan, Fu-Mei Chen, Xiu-Ling Zhao, Huan-Jing Wang, Peng-Fei Zhang, Feng-Ling Huang, Yang-Qing Lu, Ming Zhang
The testicular seminiferous tubules contain Sertoli cells and different types of spermatogenic cells. They provide the microenvironment for spermatogenesis, but the precise molecular mechanism of spermatogenesis is still not well known. Here, we have employed tandem mass tag coupled to LC-MS/MS with the high-throughput quantitative proteomics technology to explore the protein expression from buffalo testicular seminiferous tubules at three different developmental stages (prepuberty, puberty, and postpuberty)...
July 2016: Proteomics
https://www.readbyqxmd.com/read/26680493/differences-in-bisphenol-a-and-estrogen-levels-in-the-plasma-and-seminal-plasma-of-men-with-different-degrees-of-infertility
#4
J Vitku, L Sosvorova, T Chlupacova, R Hampl, M Hill, V Sobotka, J Heracek, M Bicikova, L Starka
The general population is potentially exposed to many chemicals that can affect the endocrine system. These substances are called endocrine disruptors (EDs), and among them bisphenol A (BPA) is one of the most widely used and well studied. Nonetheless, there are still no data on simultaneous measurements of various EDs along with steroids directly in the seminal fluid, where deleterious effects of EDs on spermatogenesis and steroidogenesis are assumed. We determined levels of BPA and 3 estrogens using LC-MS/MS in the plasma and seminal plasma of 174 men with different degrees of infertility...
2015: Physiological Research
https://www.readbyqxmd.com/read/26208397/interaction-between-basigin-and-monocarboxylate-transporter-2-in-the-mouse-testes-and-spermatozoa
#5
Cheng Chen, Mamiko Maekawa, Kenji Yamatoya, Masami Nozaki, Chizuru Ito, Toshihiko Iwanaga, Kiyotaka Toshimori
Basigin is a member of the immunoglobulin superfamily and plays various important roles in biological events including spermatogenesis. To examine the basigin molecular variants during spermatogenesis and sperm maturation in the mouse, immunoprecipitated basigin samples from testis and epididymal spermatozoa were analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The results demonstrated that basigin molecules from the testis and spermatozoa were separable into two major bands and that the differences in the molecular sizes were possibly because of an endoproteolytic cleavage...
July 2016: Asian Journal of Andrology
https://www.readbyqxmd.com/read/25603787/bovine-sperm-plasma-membrane-proteomics-through-biotinylation-and-subcellular-enrichment
#6
Sergo Kasvandik, Gerly Sillaste, Agne Velthut-Meikas, Aavo-Valdur Mikelsaar, Triin Hallap, Peeter Padrik, Tanel Tenson, Ülle Jaakma, Sulev Kõks, Andres Salumets
A significant proportion of mammalian fertilization is mediated through the proteomic composition of the sperm surface. These protein constituents can present as biomarkers to control and regulate breeding of agricultural animals. Previous studies have addressed the bovine sperm cell apical plasma membrane (PM) proteome with nitrogen cavitation enrichment. Alternative workflows would enable to expand the compositional data more globally around the entire sperm's surface. We used a cell surface biotin-labeling in combination with differential centrifugation to enrich sperm surface proteins...
June 2015: Proteomics
https://www.readbyqxmd.com/read/25044914/proteomics-analysis-of-adult-testis-from-bombyx-mori
#7
Yan Zhang, Zhaoming Dong, Peiming Gu, Weiwei Zhang, Dandan Wang, Xiaomeng Guo, Ping Zhao, Qingyou Xia
The development of the testis involves a large number of tissue-specific proteins, possibly because the sperms in it are the most divergent of all cell types. In this study, LC-MS/MS was employed to investigate the protein compositions of the adult testis of silkworm. A total of 14,431 peptides were identified in the adult testis of Bombyx mori, which were matched to 2292 proteins. Thirty-two HSPs constitute a group of most abundant proteins in the adult testis, suggesting that they are critical for the development, differentiation, and survival of germ cells...
October 2014: Proteomics
https://www.readbyqxmd.com/read/24781426/high-throughput-sperm-differential-proteomics-suggests-that-epigenetic-alterations-contribute-to-failed-assisted-reproduction
#8
Rubén Azpiazu, Alexandra Amaral, Judit Castillo, Josep Maria Estanyol, Marta Guimerà, Josep Lluís Ballescà, Juan Balasch, Rafael Oliva
STUDY QUESTION: Are there quantitative alterations in the proteome of normozoospermic sperm samples that are able to complete IVF but whose female partner does not achieve pregnancy? SUMMARY ANSWER: Normozoospermic sperm samples with different IVF outcomes (pregnancy versus no pregnancy) differed in the levels of at least 66 proteins. WHAT IS KNOWN ALREADY: The analysis of the proteome of sperm samples with distinct fertilization capacity using low-throughput proteomic techniques resulted in the detection of a few differential proteins...
June 2014: Human Reproduction
https://www.readbyqxmd.com/read/24716618/parameters-of-the-reproductive-tract-spermatogenesis-daily-sperm-production-and-major-seminal-plasma-proteins-of-tropically-adapted-morada-nova-rams
#9
F M L Sousa, C H Lobo, E S B Menezes, J P A Rego, R V Oliveira, A C Lima-Souza, M Fioramonte, F C Gozzo, R C F F Pompeu, M J D Cândido, J T Oliveira, A A Moura
This study describes the reproductive parameters of Morada Nova rams, a breed of hair sheep from Brazil and with unique adaption to tropical environments. At 42 weeks of age, 15 rams were subjected to semen collection and, 1 week later, animals were slaughtered for collection of testes, epididymis and accessory sex glands. We conducted 2-D electrophoresis of seminal plasma proteins and major spots of stained gels were identified by LC-MS/MS. Total RNA was isolated from testis, epididymis and vesicular glands and subjected to qPCR...
June 2014: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/24524833/levels-of-the-retinoic-acid-synthesizing-enzyme-aldehyde-dehydrogenase-1a2-are-lower-in-testicular-tissue-from-men-with-infertility
#10
MULTICENTER STUDY
John K Amory, Samuel Arnold, María C Lardone, Antonio Piottante, Mauricio Ebensperger, Nina Isoherranen, Charles H Muller, Thomas Walsh, Andrea Castro
OBJECTIVE: To determine whether decreased testicular levels of enzymes necessary for retinoic acid biosynthesis were associated with male infertility, as retinoic acid is known to be necessary for spermatogenesis. DESIGN: Observational analysis of testicular tissue samples, sperm indices, and serum hormone concentrations. SETTING: Two infertility centers in Chile. PATIENT(S): 32 infertile men and 11 control men. INTERVENTION(S): Measurement of the three enzymes necessary for retinoic acid biosynthesis, aldehyde dehydrogenase (ALDH) 1A1, 1A2, and 1A3, in testicular tissue by a novel liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) peptide assay...
April 2014: Fertility and Sterility
https://www.readbyqxmd.com/read/24422521/secretome-analysis-of-testicular-peritubular-cells-a-window-into-the-human-testicular-microenvironment-and-the-spermatogonial-stem-cell-niche-in-man
#11
Florian Flenkenthaler, Stefanie Windschüttl, Thomas Fröhlich, J Ullrich Schwarzer, Artur Mayerhofer, Georg J Arnold
Spermatogonial stem cells (SSCs) are vital for lifelong spermatogenesis in man. In their niches, a special growth factor milieu and structural support by surrounding cells are thought to ensure their maintenance. In man, the cells of the wall of seminiferous tubules, human testicular peritubular cells (HTPCs), are considered to contribute to this microenvironment and the overall testicular microenvironment via secreted proteins. Therefore, the secretome of cultured HTPCs from five individual men was analyzed by LC-MS/MS...
March 7, 2014: Journal of Proteome Research
https://www.readbyqxmd.com/read/23800682/testicular-phosphoproteome-in-perfluorododecanoic-acid-exposed-rats
#12
Zhimin Shi, Junjie Hou, Xuejiang Guo, Hongxia Zhang, Fuquan Yang, Jiayin Dai
Perfluorododecanoic acid (PFDoA) is a common environmental pollutant, which has been detected in human sera and has adverse effects on testicular function in animal models. Exploring phosphorylation events in testes helps elucidate the specific phosphorylation signals involved in testicular toxicity of PFDoA. Combining efficient prefractionation of tryptic peptide mixtures using self-packed reversed phase C18 columns with TiO2 and IMAC phosphopeptide enrichment techniques followed by 2D-LC-MS/MS, we identified 4077 unique phosphopeptides from 1777 proteins with a false discovery rate below 1...
August 14, 2013: Toxicology Letters
https://www.readbyqxmd.com/read/23413144/intratesticular-13-cis-retinoic-acid-is-lower-in-men-with-abnormal-semen-analyses-a-pilot-study
#13
J J Nya-Ngatchou, S L M Arnold, T J Walsh, C H Muller, S T Page, N Isoherranen, J K Amory
Intratesticular retinoic acid is necessary for spermatogenesis, but the relationship between intratesticular retinoic acid and sperm quality in man has not been studied. We hypothesized that intratesticular concentrations of retinoic acid would be lower in men with abnormal semen analyses compared to men with normal semen analyses. We recruited men requiring scrotal or penile surgery in a pilot observational study examining the relationship between sperm quality and intratesticular and serum retinoic acid. Twenty-four men provided two pre-operative blood and semen samples, and underwent a testicular biopsy during surgery...
March 2013: Andrology
https://www.readbyqxmd.com/read/23072795/proteomics-in-reproductive-biology-beacon-for-unraveling-the-molecular-complexities
#14
REVIEW
Rahul D Upadhyay, N H Balasinor, Anita V Kumar, Geetanjali Sachdeva, Priyanka Parte, Kushaan Dumasia
Proteomics, an interface of rapidly evolving advances in physics and biology, is rapidly developing and expanding its potential applications to molecular and cellular biology. Application of proteomics tools has contributed towards identification of relevant protein biomarkers that can potentially change the strategies for early diagnosis and treatment of several diseases. The emergence of powerful mass spectrometry-based proteomics technique has added a new dimension to the field of medical research in liver, heart diseases and certain forms of cancer...
January 2013: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/22332119/identification-of-testis-14-3-3-binding-proteins-by-tandem-affinity-purification
#15
Pawan Puri, Amparo Acker-Palmer, Ryan Stahler, Yijing Chen, Douglas Kline, Srinivasan Vijayaraghavan
The 14-3-3 family of proteins interacts with various cellular phosphoproteins and regulates multiple cell signaling cascades. Identification of 14-3-3 interactors is important to define 14-3-3 functions in various biological pathways. The binding partners of protein 14-3-3 in testis are not known. The main goal of this study was to identify the 14-3-3 interactome in testis to determine the 14-3-3 regulated cellular processes in testis. We used transgenic mice expressing tandem affinity tagged 14-3-3ζ (TAP-14-3-3ζ) driven by the ubiquitin promoter to isolate 14-3-3 binding proteins...
2011: Spermatogenesis
https://www.readbyqxmd.com/read/22205278/stk31-tdrd8-is-dynamically-regulated-throughout-mouse-spermatogenesis-and-interacts-with-miwi-protein
#16
Jianqiang Bao, Li Wang, Jianbo Lei, Yanqin Hu, Yue Liu, Hongbin Shen, Wei Yan, Chen Xu
Tudor-domain-containing proteins (TDRDs) are suggested to be critical regulators of germinal granules assembly involved in Piwi-interacting RNAs (piRNAs)-mediated pathways, of which associated components and the underlying functional mechanisms, however, remain to be elucidated. We herein characterized the expression pattern of STK31, a member of TDRDs subfamily (also termed as TDRD8), throughout spermatogenesis during mouse postnatal development. RT-PCR and Western blot verified its preferential expression in testis, but not in any other somatic tissues, in addition to embryonic stem cells...
March 2012: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/22172944/tumor-suppressor-cell-adhesion-molecule-1-cadm1-is-cleaved-by-a-disintegrin-and-metalloprotease-10-adam10-and-subsequently-cleaved-by-%C3%AE-secretase-complex
#17
Yusuke Nagara, Man Hagiyama, Naoya Hatano, Eugene Futai, Satoshi Suo, Yutaka Takaoka, Yoshinori Murakami, Akihiko Ito, Shoichi Ishiura
Cell adhesion molecule 1 (CADM1) is a type I transmembrane glycoprotein expressed in various tissues. CADM1 is a cell adhesion molecule with many functions, including roles in tumor suppression, apoptosis, mast cell survival, synapse formation, and spermatogenesis. CADM1 undergoes membrane-proximal cleavage called shedding, but the sheddase and mechanisms of CADM1 proteolysis have not been reported. We determined the cleavage site involved in CADM1 shedding by LC/MS/MS and showed that CADM1 shedding occurred in the membrane fraction and was inhibited by tumor necrosis factor-α protease inhibitor-1 (TAPI-1)...
January 6, 2012: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/21949745/first-transcriptome-of-the-testis-vas-deferens-male-accessory-gland-and-proteome-of-the-spermatophore-from-dermacentor-variabilis-acari-ixodidae
#18
Daniel E Sonenshine, Brooke W Bissinger, Noble Egekwu, Kevin V Donohue, Sayed M Khalil, R Michael Roe
Ticks are important vectors of numerous human diseases and animal diseases. Feeding stimulates spermatogenesis, mating and insemination of male factors that trigger female reproduction. The physiology of male reproduction and its regulation of female development are essentially a black box. Several transcriptomes have catalogued expression of tick genes in the salivary glands, synganglion and midgut but no comprehensive investigation has addressed male reproduction and mating. Consequently, a new global approach using transcriptomics, proteomics, and quantitative gene expression is needed to understand male reproduction and stimulation of female reproduction...
2011: PloS One
https://www.readbyqxmd.com/read/21800909/proteomic-analysis-of-insecticide-triazophos-induced-mating-responsive-proteins-of-nilaparvata-lugens-st%C3%A3-l-hemiptera-delphacidae
#19
Lin-Quan Ge, Yao Cheng, Jin-Cai Wu, Gary C Jahn
The brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is a classic example of a resurgent pest induced by insecticides. It has been demonstrated that triazophos treatment causes an increase in the content of male accessory gland proteins (Acps) that can be transferred to females via mating, influencing female reproduction. However, the mechanism of this type of insecticide-induced Acps in males and the subsequent stimulation of reproduction in females are not well understood. To identify changes in the types of Acps and reproductive proteins in mated females, we conducted a comparative proteomic analysis...
October 7, 2011: Journal of Proteome Research
https://www.readbyqxmd.com/read/21076435/proteomics-of-spermatogenesis-from-protein-lists-to-understanding-the-regulation-of-male-fertility-and-infertility
#20
REVIEW
Xiao-Yan Huang, Jia-Hao Sha
Proteomic technologies have undergone significant development in recent years, which has led to extensive advances in protein research. Currently, proteomic approaches have been applied to many scientific areas, including basic research, various disease and malignant tumour diagnostics, biomarker discovery and other therapeutic applications. In addition, proteomics-driven research articles examining reproductive biology and medicine are becoming increasingly common. The key challenge for this field is to move from lists of identified proteins to obtaining biological information regarding protein function...
January 2011: Asian Journal of Andrology
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