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Lactate Dehydrogenase

Naveen K Singh, Sunil K Arya, Pedro Estrela, Pranab Goswami
A capacitive aptasensor for detecting the malaria biomarker, Plasmodium falciparum glutamate dehydrogenase (PfGDH), directly in human serum samples developed. A thiolated ssDNA aptamer (NG3) that binds specifically to PfGDH antigen with high affinity (Kd = 79 nM) was used to develop the aptasensor. The aptasensor produced capacitance response at an optimized frequency of 2 Hz in a non-Faradaic electrochemical impedance based signal transduction platform. The aptasensor exhibited a wide dynamic range of 100 fM-100 nM with a limits of detection of 0...
June 8, 2018: Biosensors & Bioelectronics
Bai Xuejuan, Wang Dongfang, Yinping Liu, Xiao Li, Yan Liang, Yourong Yang, Junxian Zhang, Minggui Lin, Wu Xueqiong
There is an urgent need for a more effective vaccine against tuberculosis (TB). Cytotoxic T lymphocytes (CTLs) play a critical role in combating Mycobacterium tuberculosis (M.tb). The identification of novel CTL epitopes is essential for the design of peptide-based vaccines. In this study, we predicted CTL epitope peptides of M.tb antigen Rv2629 restricted by HLA-A2, using bioinformatics methods. The affinity and stability of binding of these peptides with HLA-A2 molecules were detected by flow cytometry. Their ability to induce CTLs generation was determined in peripheral blood mononuclear cells (PBMCs) from healthy uninfected subjects, Latent tuberculosis infection (LTBI) subjects, and TB patients ex vivo...
June 14, 2018: Immunology Letters
L Han, F Batistel, Y Ma, A S M Alharthi, C Parys, J J Loor
The periparturient period is the most critical period during the lactation cycle of dairy cows and is characterized by increased oxidative stress status. The objective of this experiment was to evaluate the effect of supplementing rumen-protected methionine on nuclear factor erythroid 2-like 2 (NFE2L2, formerly NRF2) protein and target gene expression in the mammary gland during the early postpartal period. Multiparous Holstein cows were used in a block design experiment with 30 cows per treatment. Treatments consisting of a basal control diet (control) or the basal diet plus rumen-protected methionine (methionine) were fed from d -28 to 60 relative to parturition...
June 13, 2018: Journal of Dairy Science
Yongfeng Deng, Yan Zhang, Ruxia Qiao, Melvin M Bonilla, Xiaoliang Yang, Hongqiang Ren, Bernardo Lemos
This study was performed to reveal the health risks of co-exposure to organophosphorus flame retardants (OPFRs) and microplastics (MPs). We exposed mice to polyethylene (PE) and polystyrene (PS) MPs and OPFRs [tris (2-chloroethy) phosphate (TCEP) and tris (1,3-dichloro-2-propyl) phosphate (TDCPP)] for 90 days. Biochemical markers and metabolomics were used to determine whether MPs could enhance the toxicity of OPFRs. Superoxide dismutase (SOD) and catalase (CAT) increased (p < 0.05) by 21% and 26% respectively in 10 μg/L TDCPP + PE group compared to TDCPP group...
June 8, 2018: Journal of Hazardous Materials
Bingrui Ma, Naling Yu, Yuetong Han, Mengchun Gao, Sen Wang, Shanshan Li, Liang Guo, Zonglian She, Yangguo Zhao, Chunji Jin, Feng Gao
The performance, microbial enzymatic activity and microbial community of a sequencing batch reactor (SBR) have been explored under magnesium oxide nanoparticles (MgO NPs) stress. The NH4 + -N removal efficiency kept relatively stable during the whole operational process. The MgO NPs at 30-60 mg/L slightly restrained the removal of chemical oxygen demand (COD), and the presence of MgO NPs also affected the denitrification and phosphorus removal. The specific oxygen uptake rate, nitrifying and denitrifying rates, phosphorus removal rate, and microbial enzymatic activities distinctly varied with the increase of MgO NPs concentration...
June 13, 2018: Journal of Environmental Management
Wen-Yan Zhu, Xin Jin, Yong-Chi Ma, Zhi-Biao Liu
Ischemia and oxidative stress play crucial roles in the pathophysiology of sudden sensorineural hearing loss (SSNHL). Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine and serves an important role in hearing function. The present study was designed to evaluate the effect of MIF on oxygen-glucose deprivation (OGD)-induced ototoxicity and to elucidate its molecular mechanism. In HEI-OC1 auditory cells, OGD reduced cell viability and increased supernatant lactate dehydrogenase (LDH) and MIF in a time-dependent manner...
June 13, 2018: Biochemical and Biophysical Research Communications
Ryoko Suzuki, Xiong Wei, Pamela K Allen, James W Welsh, Ritsuko Komaki, Steven H Lin
BACKGROUND AND PURPOSE: We sought factors associated with the development of brain metastases after treatment of small cell lung cancer (SCLC) in patients without brain involvement at diagnosis. METHODS: We analyzed 293 patients with SCLC without brain metastases who received chemotherapy, thoracic radiation therapy (TRT), or both in 2001-2015. Pretreatment hematologic markers (platelet count, neutrophil count, lymphocyte count, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and lactate dehydrogenase) and other clinical characteristics were evaluated for correlation with brain metastases-free survival (BMFS)...
June 12, 2018: Radiotherapy and Oncology: Journal of the European Society for Therapeutic Radiology and Oncology
Jun Lu, Zhonghong Wei, Hua Jiang, Lu Cheng, Qiuhua Chen, Mingqi Chen, Jing Yan, Zhiguang Sun
BACKGROUND: Sepsis is a major health care problem, which affects millions of people around the world. Glucose metabolic reprogramming of immune cells plays a crucial role during advancement of sepsis. However, the association between glucose metabolic reprogramming and mortality in patients with sepsis is unclear. Lactate dehydrogenase (LDH) catalyzes the last step of glycolysis. Investigating the relationship between LDH and mortality is important to understand the effect of metabolic reprogramming on prognosis of patients with sepsis...
August 2018: Journal of Surgical Research
Baek-Rock Oh, Sung-Mok Lee, Sun-Yeon Heo, Jeong-Woo Seo, Chul Ho Kim
BACKGROUND: 1,3-Propanediol (1,3-PDO) is important building blocks for the bio-based chemical industry, Klebsiella pneumoniae can be an attractive candidate for their production. However, 1,3-PDO production is high but productivity is generally low by K. pneumoniae. In this study, repeated fed-batch cultivation by a lactate and 2,3-butanediol (2,3-BDO) deficient mutant of K. pneumoniae were investigated for efficient 1,3-PDO production from industrial by-products such as crude glycerol...
June 15, 2018: Microbial Cell Factories
Xisi Wang, Lijun Wang, Yan Su, Zhixia Yue, Tianyu Xing, Wen Zhao, Qian Zhao, Chao Duan, Cheng Huang, Dawei Zhang, Mei Jin, Xianfeng Cheng, Shenglan Chen, Yi Liu, Xiaoli Ma
To evaluate plasma cell-free DNA (cfDNA) as a promising biomarker for neuroblastoma (NB) tumor burden. Seventy-nine eligible patients with newly diagnosed NB were recruited from Beijing Children's Hospital between April 2016 and April 2017. Additionally, from September 2011 to June 2017, 79 patients with stable NB were evaluated with a median follow-up time of 21 months. Approximately 2 mL of peripheral blood was drawn upon enrollment, and plasma cfDNA levels were measured via quantitative polymerase chain reaction (qPCR)...
June 14, 2018: Cancer Medicine
S Ran, M Chu, S Gu, J Wang, J Liang
AIM: To investigate the ability of E. faecalis to cause apoptosis and pyroptosis of human osteoblastic MG63 cells and whether the inflammasome is involved in this process. METHODOLOGY: MG63 cells were infected with E. faecalis at a multiplicity of infection (MOI) of 10, 100, 500, and 1000 for 6 and 12 h. The cell proliferation was determined with the Cell Counting Kit-8. Apoptosis and pyroptosis after E. faecalis infection was evaluated by flow cytometry and a lactate dehydrogenase (LDH) cytotoxicity assay kit...
June 14, 2018: International Endodontic Journal
Tomasz Walski, Anna Drohomirecka, Jolanta Bujok, Albert Czerski, Grzegorz Wąż, Natalia Trochanowska-Pauk, Michał Gorczykowski, Romuald Cichoń, Małgorzata Komorowska
Aim: An activation of non-specific inflammatory response, coagulation disorder, and blood morphotic elements damage are the main side effects of the extracorporeal circulation (ECC). Red-to-near-infrared radiation (R/NIR) is thought to be capable of stabilizing red blood cell (RBC) membrane through increasing its resistance to destructive factors. We focused on the development of a method using low-level light therapy (LLLT) in the spectral range of R/NIR which could reduce blood trauma caused by the heart-lung machine during surgery...
2018: Frontiers in Physiology
Gilad Barshad, Amit Blumberg, Tal Cohen, Dan Mishmar
Oxidative phosphorylation (OXPHOS), a fundamental energy source in all human tissues, requires interactions between mitochondrial (mtDNA) and nuclear (nDNA)-encoded protein subunits. Although such interactions are fundamental to OXPHOS, bi-genomic co-regulation is poorly understood. To address this question, we analyzed ~8,500 RNA-seq experiments from 48 human body sites. Despite well-known variation in mitochondrial activity, quantity and morphology, we found overall positive mtDNA-nDNA OXPHOS genes' co-expression across human tissues...
June 14, 2018: Genome Research
Zhaoshuo Lu, Jingli Zhu, Xinxin Wang, Jian Yao, Yue Wu, Decai Wang
OBJECTIVE: To study the neurotoxicity and mechanism of polychlorinated biphenyl( PCB) in BV-2 cells. METHODS: The experiment was divided into control group and PCB118, 138, 153, 180 treated group, the dosages were 0. 000, 0. 015, 0. 030, 0. 050, 0. 100, 0. 150 μmol/L. Through the statistical analysis of the survival rate of the cells, the final dose was divided into low, middle and high dose levels. Among them, the high dose group except PCB118 was 0. 15 μmol/L, the PCB138, 153, 180 concentration was 0...
January 2018: Wei Sheng Yan Jiu, Journal of Hygiene Research
Jinlian Li, Na Li, Xiaoqing Dong, Jing Yang, Haiyun Luan
OBJECTIVE: To investigate whether the protective effects of L-carnitine( LC) against hydrogen peroxide( H_2O_2)-induced injury in hepatocytes were related to nuclear factor-kappa B( NF-κB). METHODS: CCK-8 and lactate dehydrogenase( LDH)methods were used to detect the influences of NF-κB inhibitors on the cell damage induced by H_2O_2. The effects of LC on the NF-κB expressions in H_2O_2-treated HL7702 cells were determined by Western blot. The translocation of NF-κB was observed by immunofluorescence staining...
July 2017: Wei Sheng Yan Jiu, Journal of Hygiene Research
Tomáš Smutný, Riina Harjumäki, Liisa Kanninen, Marjo Yliperttula, Petr Pávek, Yan-Ru Lou
Herbal medicines have been increasingly used in the last three decades. Despite their popularity, safety issues with herbal products need to be addressed. We performed a feasibility study of the toxic responses of human induced pluripotent stem cell-derived hepatocytes (iHep cells) to phytochemicals in comparison with hepatoblasoma-derived HepG2 cells and long-term human hepatocytes (LTHHs). The iHep cells expressed typical hepatocyte markers cytochrome P450 3A4 (CYP3A4), hepatocyte nuclear factor 4α, and albumin despite the expression of immature markers α-fetoprotein and cytokeratin 19...
June 11, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Chunfu Wan, Yueyue Wei, Jianguo Ma, Xiaoyong Geng
The present study aimed to investigate the protective effects and molecular mechanisms of scoparone on ischemia‑reperfusion (I/R) injury in primary cultured cardiac myocytes and rats. An in vivo rat model of I/R injury and an in vitro primary cultured cardiac myocyte model of oxygen‑glucose deprivation/reoxygenation were used to investigate the protective effects of scoparone. Cell viability, lactate dehydrogenase (LDH) release, superoxide dismutase (SOD), creatine kinase (CK) and malondialdehyde (MDA) levels, and reactive oxygen species (ROS) production were subsequently measured...
June 4, 2018: Molecular Medicine Reports
Satoshi Sugita, Hideki Enokida, Hirofumi Yoshino, Kazutaka Miyamoto, Masaya Yonemori, Takashi Sakaguchi, Yoichi Osako, Masayuki Nakagawa
The active form of the small GTPase RAS binds to downstream effectors to promote cell growth and proliferation. RAS signal enhancement contributes to tumorigenesis, invasion, and metastasis in various different cancers. HRAS proto-oncogene GTPase (HRAS), one of the RAS isoforms, was the first human oncogene for which mutations were reported in T24 bladder cancer (BC) cells in 1982, and HRAS mutation or upregulation has been reported in several cancers. According to data from The Cancer Genome Atlas, HRAS expression was significantly upregulated in clinical BC samples compared to healthy samples (P=0...
June 11, 2018: International Journal of Oncology
Jarunee Leetheeragul, Dittakarn Boriboonhirunsarn, Kanit Reesukumal, Nusara Srisaimanee, Siriluck Horrasith, Tuangsit Wataganara
INTRODUCTION: There is little information on the effect of maternal characteristics and on-admission laboratory parameters to the therapeutic serum magnesium sulfate (MgSO4) levels in women with preeclampsia (PE). We sought to identify factors that may predict timely attainment of therapeutic serum magnesium levels after intravenous administration for seizure prophylaxis. MATERIALS AND METHODS: On-admission factors of 360 women with PE who received intravenous MgSO4 (4-g loading and 2-g/hr maintenance) for seizure prophylaxis were retrospectively reviewed...
June 13, 2018: Journal of Maternal-fetal & Neonatal Medicine
Søren Kristiansen, Lennart Friis-Hansen, Claus Antonio Juel Jensen, Steen Ingemann Hansen
We verified the lactate dehydrogenase (LDH) reference interval (RI) provided by the Nordic Reference Interval Project (NORIP). The serum LDH concentration was analysed on the Dimension Vista 1500 system with an IFCC method with a bias of +2.1 % and +2.7 % against NFKK Reference Serum X and ERM-AD453/IFCC, respectively, showing verification of transference of the NORIP RI. Selective data mining in clinical laboratory information systems for retrospective serum LDH test results was used to calculate an indirect RI...
June 13, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
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