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Lactate Dehydrogenase

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https://www.readbyqxmd.com/read/29458133/artesunate-derived-monomeric-dimeric-and-trimeric-experimental-drugs-their-unique-mechanistic-basis-and-pronounced-antiherpesviral-activity
#1
Friedrich Hahn, Tony Fröhlich, Theresa Frank, Luca D Bertzbach, Stephan Kohrt, Benedikt B Kaufer, Thomas Stamminger, Svetlana B Tsogoeva, Manfred Marschall
Human cytomegalovirus (HCMV) is a major human pathogen and is associated with severe pathology, such as life-threatening courses of infection in immunocompromised individuals and neonates. Currently, antiviral therapy is still hampered by a considerable toxicity of the available drugs and induction of viral resistance. Recently, we and others reported the very potent antiviral activity of the broad antiinfective drug artesunate in vitro and in vivo. Here, we investigated further optimized analogs including monomeric, dimeric and trimeric derivatives belonging to this highly interesting chemical group of experimental drugs (sesquiterpenes/trioxanes) and compared these to the previously identified trimeric artesunate compound TF27...
February 16, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29458021/increased-mortality-from-influenza-infection-in-long-chain-acyl-coa-dehydrogenase-knockout-mice
#2
Apurva Shinde, Jiadi Luo, Sivakama S Bharathi, Huifang Shi, Megan E Beck, Kevin J McHugh, John F Alcorn, Jieru Wang, Eric S Goetzman
We previously showed that the mitochondrial fatty acid oxidation enzyme long-chain acyl-CoA dehydrogenase (LCAD) is expressed in alveolar type II pneumocytes and that LCAD-/- mice have altered breathing mechanics and surfactant defects. Here, we hypothesized that LCAD-/- mice would be susceptible to influenza infection. Indeed, LCAD-/- mice demonstrated increased mortality following infection with 2009 pandemic influenza (A/CA/07/09). However, the mortality was not due to increased lung injury, as inflammatory cell counts, viral titers, and histology scores all showed non-significant trends toward milder injury in LCAD-/- mice...
February 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29457737/determining-the-value-of-cerebrospinal-fluid-lactate-dehydrogenase-level-in-differentiating-subarachnoid-hemorrhage-from-traumatic-lumbar-puncture
#3
Morteza Saeedi, Elnaz Vahidi, Shirin Asri, Amirhosein Jahanshir
CONTEXT: - Lumbar puncture (LP) is still an important modality in the diagnosis of subarachnoid hemorrhage (SAH). Rapid and correct fluid analysis can provide patients with a better prognosis by appropriate intervention. OBJECTIVE: - To determine the value of cerebrospinal fluid lactate dehydrogenase level in differentiation between SAH and traumatic LP. DESIGN: - This was a cross-sectional observational study. Patients with a diagnostic suspicion of SAH, who were admitted to the emergency department, were enrolled in our study based on the inclusion criteria...
February 19, 2018: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/29456986/extract-from-teucrium-polium-l-protects-rat-heart-against-oxidative-stress-induced-by-ischemic-reperfusion-injury
#4
Maryam Mahmoudabady, Milad Haghshenas, Saeed Niazmand
Background: The deleterious effect of oxidative stress on myocardial ischemia-reperfusion (I/R) has already been shown in previous studies. Since Teucrium polium has anti-oxidative and cardio-protective properties, the aim of this study was to investigate the effects of this plant on I/R injuries in the isolated rat heart. Materials and Methods: The myocardial I/R injury of rat was created by Langendorff retrograde perfusion technology. The heart was preperfused with Krebs-Henseleit (K-H) solution containing T...
2018: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29455176/late-diagnosis-a-case-of-rapidly-progressive-extranodal-nk-t-cell-lymphoma-nasal-type
#5
Hiroyuki Mori, Kei Ebisawa, Mitsushige Nishimura, Kenji Kanazawa
Extranodal natural killer (NK)/T cell lymphoma, nasal type is a condition that has poor prognosis. Accurate diagnosis of lymphoma is made by pathological findings. We report a case of extranodal NK/T cell lymphoma, nasal type affecting the lung and liver and which was difficult to diagnose because of negative biopsy results from multiple sites. A 39-year-old man who had dry cough and fever for 1 month was referred to our hospital. He had pancytopenia and elevated serum levels of lactate dehydrogenase and soluble interleukin-2 receptor...
February 17, 2018: BMJ Case Reports
https://www.readbyqxmd.com/read/29454865/inhibitory-effects-of-selenium-on-cadmium-induced-cytotoxicity-in-pc12-cells-via-regulating-oxidative-stress-and-apoptosis
#6
Kaniz Fatima Binte Hossain, Md Mostafizur Rahman, Md Tajuddin Sikder, Takeshi Saito, Toshiyuki Hosokawa, Masaaki Kurasaki
Purpose of this study is to investigate mechanism/s of cyto-protection by selenium (Na2 SeO3 ; Se4+ ) against cadmium (CdCl2 ; Cd2+ )-induced cytotoxicity using PC12 cells. In addition, Se (5, 10, 20 and 40 μM) and Cd (2.5, 5 and 10 μM)-induced cytotoxicity is determined. Cytotoxicity assays and western blot analyses confirmed that Se (≥10 μM) promotes autophagic cell death via inhibition of mTOR activation and p62 accumulation due to increase of cellular oxidative stress. On the other hand, co-presence of non-toxic Se (5 μM) and toxic Cd (5 μM) showed to increase cell viability, glutathione and glutathione peroxidase 1 (GPx1) levels, and to decrease DNA fragmentation and lactate dehydrogenase (LDH) activity compared to Cd-treated (5 μM) cells alone...
February 15, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29454001/brain-bioenergetics-in-rats-with-acute-hyperphenylalaninemia
#7
Nádia Weber Dimer, Bruna Klippel Ferreira, Jotele Fontana Agostini, Maria Luiza Gomes, Luiza Wilges Kist, Fernanda Malgarin, Milena Carvalho-Silva, Lara Mezari Gomes, Joyce Rebelo, Marisa Jádna Silva Frederico, Fátima Regina Mena Barreto Silva, Eduardo Pacheco Rico, Mauricio Reis Bogo, Emilio Luiz Streck, Gustavo Costa Ferreira, Patrícia Fernanda Schuck
Phenylketonuria (PKU) is a disorder of phenylalanine (Phe) metabolism caused by deficient phenylalanine hydroxylase activity. The deficiency results in increased levels of Phe and its metabolites in fluids and tissues of patients. PKU patients present neurological signals and symptoms including hypomyelination and intellectual deficit. This study assessed brain bioenergetics at 1 h after acute Phe administration to induce hyperphenylalaninemia (HPA) in rats. Wistar rats were randomized in two groups: HPA animals received a single subcutaneous administration of Phe (5...
February 14, 2018: Neurochemistry International
https://www.readbyqxmd.com/read/29449156/stochastic-expression-of-lactate-dehydrogenase-a-induces-escherichia-coli-persister-formation
#8
Naoki Yamamoto, Rino Isshiki, Yuto Kawai, Daiki Tanaka, Tetsushi Sekiguchi, Shinya Matsumoto, Satoshi Tsuneda
Bacterial persisters are phenotypic variants that survive the treatment of lethal doses of growth-targeting antibiotics without mutations. Although the mechanism underlying persister formation has been studied for decades, how the persister phenotype is switched on and protects itself from antibiotics has been elusive. In this study, we focused on the lactate dehydrogenase gene (ldhA) that was upregulated in an Escherichia coli persister-enriched population. A survival rate assay using an ldhA-overexpressing strain showed that ldhA expression induced persister formation...
February 12, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29446169/oxygen-concentration-modulates-cellular-senescence-and-autophagy-in-human-trophoblast-cells
#9
Kotomi Seno, Nao Tanikawa, Hironori Takahashi, Akihide Ohkuchi, Hirotada Suzuki, Shigeki Matsubara, Hisataka Iwata, Takehito Kuwayama, Koumei Shirasuna
PROBLEM: We investigated the effect of oxygen concentrations on cellular senescence and autophagy and examined the role of autophagy in human trophoblast cells. METHOD OF STUDY: Human first-trimester trophoblast cells (Sw.71) were incubated under 21%, 5%, or 1% O 2 concentrations for 24 hours. We examined the extent of senescence caused using senescence-associated β-galactosidase (SA-β-Gal) and senescence-associated secretory phenotype (SASP) as markers. Moreover, we examined the role of autophagy in causing cellular senescence using an autophagy inhibitor (3-methyladenine, 3MA)...
February 15, 2018: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29446092/association-of-salivary-lactate-dehydrogenase-level-with-systemic-inflammation-in-a-japanese-population
#10
N Miyoshi, T Tanigawa, S Nishioka, K Maruyama, E Eguchi, K Tanaka, I Saito, K Yamazaki, Y Miyake
BACKGROUND AND OBJECTIVE: Salivary lactate dehydrogenase (LDH) was reported to be a useful parameter for the screening of periodontal disease. We performed a cross-sectional study to verify the usefulness of salivary LDH as a biomarker of periodontitis and to investigate the association of severity of periodontitis with systemic inflammation by measuring salivary LDH and serum high sensitive C-reactive protein (hs-CRP) levels in a community-based middle-aged and elderly population in Japan...
February 15, 2018: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29445991/rainbow-trout-oncorhynchus-mykiss-anesthesia-with-myrcene-efficacy-and-physiological-responses-in-comparison-with-eugenol
#11
Ali Taheri Mirghaed, Mahyar Yasari, Seyed Saeed Mirzargar, Seyyed Morteza Hoseini
The aim of the present study was to investigate anesthetic efficacy of myrcene in rainbow trout (Oncorhynchus mykiss) along with the fish biochemical response to anesthesia in comparison with eugenol. In the first experiment, 240 fish were stocked in 12 tanks and acclimatized to experimental conditions for 2 weeks. Then, the fish of each tank were subjected to one concentration of either eugenol (12, 20, 30, 50, 80, and 130 μL/L) or myrcene (100, 150, 200, 300, 400, and 500 μL/L) concentrations. Induction time of and recovery time from anesthesia were recorded for each fish separately...
February 14, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29444941/lactic-acid-downregulates-viral-microrna-to-promote-epstein-barr-virus-immortalized-b-lymphoblastic-cell-adhesion-and-growth
#12
Xiaohui Mo, Fang Wei, Yin Tong, Ling Ding, Qing Zhu, Shujuan Du, Fei Tan, Caixia Zhu, Yuyan Wang, Qian Yu, Yeqiang Liu, Erle S Robertson, Zhenghong Yuan, Qiliang Cai
High plasma lactate is associated with poor prognosis of many malignancies, but its role in virally mediated cancer progression and underlying molecular mechanisms are unclear. Epstein-Barr virus (EBV), the first human oncogenic virus, causes several cancers, including B cell lymphoma. Here, we report that lactate dehydrogenase (LDH-A) expression and lactate production are elevated in EBV-immortalized B lymphoblastic cells, and lactic acid (LA, acidic lactate) at low concentration triggers EBV-infected B cell adhesion, morphological changes, and proliferation in vitro and in vivo Moreover, LA-induced responses of EBV-infected B cells uniquely occurs in viral latency type III and it is dramatically associated with the inhibition of global viral microRNAs, particularly the miR-BHRF1 cluster, and the high expression of SMAD3 , JUN , and COL1A genes...
February 14, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29444744/overexpression-of-pyruvate-dehydrogenase-e1a-subunit-inhibits-warburg-effect-and-induces-cell-apoptosis-through-mitochondria-mediated-pathway-in-hepatocellular-carcinoma
#13
Jihong Sun, Jingjing Li, Zhixian Guo, Lu Sun, Chenghui Juan, Yubing Zhou, Hongli Gu, Yan Yu, Qiuyue Hu, Quancheng' Kan, Zujiang Yu
Most cancers rely disproportionately on glycolysis for energy even in the presence of adequate oxygen supply, a condition known as "aerobic glycolysis", or the "Warburg effect". Pyruvate dehydrogenase E1a subunit (PDHA1) is one of main factors for metabolic switch from oxidative phosphorylation (OXPHOS) to aerobic glycolysis and has been suggested to be closely associated with tumorigenesis. Here we observed that PDHA1 protein was reduced in hepatocellular carcinoma (HCC) specimens by immunohistochemistry and western blot, which was significantly associated with poor overall survival...
February 14, 2018: Oncology Research
https://www.readbyqxmd.com/read/29444683/potential-effects-of-the-combination-of-nicotinamide-vitamin-b2-and-vitamin-c-on-oxidative-mediated-hepatotoxicity-induced-by-thioacetamide
#14
Samir A E Bashandy, Hossam Ebaid, Sherif A Abdelmottaleb Moussa, Ibrahim M Alhazza, Iftekhar Hassan, Abdulaziz Alaamer, Jameel Al Tamimi
BACKGROUND: The liver disease is one of the most important traditional public health problems in Egypt. Oxidative stress is attributed to such pathological condition that further contributes to the initiation and progression of liver injury. In the present study, we have investigated if the strong antioxidant power of Nicotinamide (NA), Vitamin B2 (VB2), and Vitamin C (VC) can ameliorate TAA-induced oxidative stress-mediated liver injury in the rats. METHODS: Thirty-six albino rats were divided into six groups: Control group; TAA group (IP injection with TAA at a dosage of 200 mg/Kg three times a week for two months); TAA + NA group (rats administered with NA at a dosage of 200 mg/kg daily besides TAA as in the control); TAA + VB2 group (rats administered with vitamin B2 at a dosage of 30 mg/kg daily besides injection with TAA); TAA + VC group (rats administered with vitamin C at a dosage of 200 mg/kg daily along with injection of TAA)...
February 14, 2018: Lipids in Health and Disease
https://www.readbyqxmd.com/read/29444250/are-elevated-serum-haemolysis-markers-a-harbinger-of-adverse-events-in-heartmate-ii-patients
#15
Rashad Zayat, Mohamed Shoaib, Mohammad Amen Khattab, Usaama Ahmad, Andreas Goetzenich, Christian Stoppe, Ann Christina Foldenauer, Ajay Moza, Heike Schnoering, Rüdiger Autschbach, Lachmandath Tewarie
OBJECTIVES: Haemolysis during left ventricular assist device support is associated with thrombosis. In this retrospective study, we analysed whether low-level haemolysis (LLH) as defined by simultaneously elevated lactate dehydrogenase (LDH) and free haemoglobin (fHb) levels had an impact on thromboembolic and bleeding events and on von Willebrand factor levels in HeartMate II patients. METHODS: After exclusion of patients with LDH >700 U/l and fHb >40 mg/dl at hospital discharge, 79 HeartMate II patients were included...
February 12, 2018: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29442224/vitamin-c-and-sodium-bicarbonate-enhance-the-antioxidant-ability-of-h9c2-cells-and-induce-hsps-to-relieve-heat-stress
#16
Bin Yin, Shu Tang, Jiarui Sun, Xiaohui Zhang, Jiao Xu, Liangjiao Di, Zhihong Li, Yurong Hu, Endong Bao
Heat stress is exacerbated by global warming and affects human and animal health, leading to heart damage caused by imbalances in reactive oxygen species (ROS) and the antioxidant system, acid-base chemistry, electrolytes and respiratory alkalosis. Vitamin C scavenges excess ROS, and sodium bicarbonate maintains acid-base and electrolyte balance, and alleviates respiratory alkalosis. Herein, we explored the ability of vitamin C alone and in combination with equimolar sodium bicarbonate (Vitamin C-Na) to stimulate endogenous antioxidants and heat shock proteins (HSPs) to relieve heat stress in H9C2 cells...
February 13, 2018: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29440240/atypical-presentation-of-atypical-haemolytic-uraemic-syndrome
#17
Ratna Basak, Xiaotong Wang, Caitlin Keane, Robert Woroniecki
A 17-year-old girl presented with fever, myalgia, vomiting for 1 month and oliguria and dyspnoea for 4 days. She was tachycardic,hypertensive, with pedal oedema and decreased breath sounds. She had high serum creatinine (3 mg/dL), anaemia, thrombocytopenia, leucocytosis and eosinophilia with schistocytes. Lactate dehydrogenase, transaminases were high , with low haptoglobin and high ferritin (5269 ng/mL). Complement C3/C4 and fibrinogen were normal. Urinalysis showed large blood and protein and stool studies were negative...
February 11, 2018: BMJ Case Reports
https://www.readbyqxmd.com/read/29436842/diffusion-weighted-mri-in-myositis-the-value-of-adc-histogram-analysis
#18
Hans Jonas Meyer, Alexander Emmer, Malte Kornhuber, Alexey Surov
OBJECTIVE: Diffusion weighted imaging (DWI) has the potential of being able to reflect histopathology architecture. A novel imaging approach, namely histogram analysis, is used to further characterize tissues on MRI. The aim of this study was to correlate histogram parameters derived from ADC maps with serological parameters in myositis. METHODS: 16 patients with autoimmune myositis were included in this retrospective study. DWI was obtained on a 1.5T scanner by using the b values of 0 and 1000 s/mm2...
February 13, 2018: British Journal of Radiology
https://www.readbyqxmd.com/read/29436658/effect-of-garlic-on-rats-with-chronic-intermittent-hypoxia-combined-with-diabetes-mellitus
#19
Yinglan Peng, Ke Hu
The present study investigated the effect of garlic (G) on serum, liver, renal and cerebral parameters of rats with chronic intermittent hypoxia (CIH) combined with diabetes mellitus (DM). A total of 32 rats were divided into eight groups, with 4 rats/group. A total of three models were established, including CIH, DM and CIH‑DM, and an additional healthy control (C) group. Rats in C‑G, CIH‑G, DM‑G and CIH‑DM‑G groups were injected with a G extract daily. Serum, liver, renal and cerebral parameters were detected...
February 7, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29436603/%C3%AE-%C3%A2-lipoic-acid-can-greatly-alleviate-the-toxic-effect-of-ages-on-sh%C3%A2-sy5y-cells
#20
Guifen Niu, Jianfei Guo, Yaqiang Tian, Kexiang Zhao, Jian Li, Qian Xiao
The aim of the study was to explore the influence of α‑lipoic acid (α‑LA) on the cytotoxicity of advanced glycation end‑products (AGEs) against SH‑SY5Y cells. AGE‑bovine serum albumin (BSA) was incubated in vitro using SH‑SY5Y cells as a target model, and the control group was set. Cells were exposed to AGE‑BSA, and α‑LA was selectively added to the cells. Cell growth and death was determined by the MTT assay, which measures cellular metabolic rate, lactate dehydrogenase (LDH) leakage rate and cellular axonal length...
February 8, 2018: International Journal of Molecular Medicine
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