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https://www.readbyqxmd.com/read/29782003/collection-and-extraction-of-occupational-air-samples-for-analysis-of-fungal-dna
#1
Angela R Lemons, William G Lindsley, Brett J Green
Traditional methods of identifying fungal exposures in occupational environments, such as culture and microscopy-based approaches, have several limitations that have resulted in the exclusion of many species. Advances in the field over the last two decades have led occupational health researchers to turn to molecular-based approaches for identifying fungal hazards. These methods have resulted in the detection of many species within indoor and occupational environments that have not been detected using traditional methods...
May 2, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29781254/determination-of-carbon-isotope-ratios-for-honey-samples-by-means-of-a-liquid-chromatography-isotope-ratio-mass-spectrometry-system-coupled-with-a-post-column-pump
#2
Hiroto Kawashima, Momoka Suto, Nana Suto
RATIONALE: Liquid chromatography/isotope ratio mass spectrometry (LC/IRMS) has been used to authenticate and trace products such as honey, wine, and lemon juice, and compounds such as caffeine and pesticides. However, LC/IRMS has several disadvantages, including the high cost of the CO2 membrane and blocking by solidified sodium persulfate. Here, we developed an improved system for determining carbon isotope ratios by LC/IRMS. METHODS: The main improvement was the use of a post-column pump...
May 20, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29775709/use-of-green-chemistry-methods-in-the-extraction-of-dietary-fibers-from-cactus-rackets-opuntia-ficus-indica-structural-and-microstructural-studies
#3
Marwa Cheikh Rouhou, Souhir Abdelmoumen, Sabu Thomas, Hamadi Attia, Dorra Ghorbel
This work aims to compare the development of three green chemistry methods in the extraction of cactus (Opuntia ficus indica) dietary fibers. The influence of extraction conditions (solvent nature: water, lemon juice and ethanol; extraction process: maceration and steam explosion; extraction time: 30 min, 1 h, 3 h, 5 h) on the chemical and structural composition was studied. Fourier Transform Infrared (FTIR) spectroscopy proved that all treatments do not affect the cellulose structure and could induce the reduction of hemicelluloses and lignin content...
May 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29764719/potential-use-of-bitter-melon-momordica-charantia-derived-compounds-as-antidiabetics-in-silico-and-in-vivo-studies
#4
Olusola Olalekan Elekofehinti, Esther Opeyemi Ariyo, Moses Orimoloye Akinjiyan, Olanrewaju Sam Olayeriju, Akeem Olalekan Lawal, Isaac Gbadura Adanlawo, Joao Batista Teixeira Rocha
Momordica charantia (bitter lemon) belongs to the cucurbitaceae family which has been extensively used in traditional medicines for the cure of various ailments such as cancer and diabetes. The underlying mechanism of M. charantia to maintain glycemic control was investigated. GLP-1 and DPP-4 gene modulation by M. charantia (5-20% inclusion in rats diet) was investigated in vivo by RT-PCR and possible compounds responsible for diabetic action predicted through in silico approach. Phytochemicalss previously characterized from M...
May 12, 2018: Pathophysiology: the Official Journal of the International Society for Pathophysiology
https://www.readbyqxmd.com/read/29749849/a-faculty-professional-development-model-that-improves-student-learning-encourages-active-learning-instructional-practices-and-works-for-faculty-at-multiple-institutions
#5
Karen N Pelletreau, Jennifer K Knight, Paula P Lemons, Jill S McCourt, John E Merrill, Ross H Nehm, Luanna B Prevost, Mark Urban-Lurain, Michelle K Smith
Helping faculty develop high-quality instruction that positively affects student learning can be complicated by time limitations, a lack of resources, and inexperience using student data to make iterative improvements. We describe a community of 16 faculty from five institutions who overcame these challenges and collaboratively designed, taught, iteratively revised, and published an instructional unit about the potential effect of mutations on DNA replication, transcription, and translation. The unit was taught to more than 2000 students in 18 courses, and student performance improved from preassessment to postassessment in every classroom...
June 2018: CBE Life Sciences Education
https://www.readbyqxmd.com/read/29748285/an-anatomic-transcriptional-atlas-of-human-glioblastoma
#6
Ralph B Puchalski, Nameeta Shah, Jeremy Miller, Rachel Dalley, Steve R Nomura, Jae-Guen Yoon, Kimberly A Smith, Michael Lankerovich, Darren Bertagnolli, Kris Bickley, Andrew F Boe, Krissy Brouner, Stephanie Butler, Shiella Caldejon, Mike Chapin, Suvro Datta, Nick Dee, Tsega Desta, Tim Dolbeare, Nadezhda Dotson, Amanda Ebbert, David Feng, Xu Feng, Michael Fisher, Garrett Gee, Jeff Goldy, Lindsey Gourley, Benjamin W Gregor, Guangyu Gu, Nika Hejazinia, John Hohmann, Parvinder Hothi, Robert Howard, Kevin Joines, Ali Kriedberg, Leonard Kuan, Chris Lau, Felix Lee, Hwahyung Lee, Tracy Lemon, Fuhui Long, Naveed Mastan, Erika Mott, Chantal Murthy, Kiet Ngo, Eric Olson, Melissa Reding, Zack Riley, David Rosen, David Sandman, Nadiya Shapovalova, Clifford R Slaughterbeck, Andrew Sodt, Graham Stockdale, Aaron Szafer, Wayne Wakeman, Paul E Wohnoutka, Steven J White, Don Marsh, Robert C Rostomily, Lydia Ng, Chinh Dang, Allan Jones, Bart Keogh, Haley R Gittleman, Jill S Barnholtz-Sloan, Patrick J Cimino, Megha S Uppin, C Dirk Keene, Farrokh R Farrokhi, Justin D Lathia, Michael E Berens, Antonio Iavarone, Amy Bernard, Ed Lein, John W Phillips, Steven W Rostad, Charles Cobbs, Michael J Hawrylycz, Greg D Foltz
Glioblastoma is an aggressive brain tumor that carries a poor prognosis. The tumor's molecular and cellular landscapes are complex, and their relationships to histologic features routinely used for diagnosis are unclear. We present the Ivy Glioblastoma Atlas, an anatomically based transcriptional atlas of human glioblastoma that aligns individual histologic features with genomic alterations and gene expression patterns, thus assigning molecular information to the most important morphologic hallmarks of the tumor...
May 11, 2018: Science
https://www.readbyqxmd.com/read/29739035/next-generation-sequencing-panel-based-on-single-molecule-molecular-inversion-probes-for-detecting-genetic-variants-in-children-with-hypopituitarism
#7
María I Pérez Millán, Sebastian A Vishnopolska, Alexandre Z Daly, Juan P Bustamante, Adriana Seilicovich, Ignacio Bergadá, Débora Braslavsky, Ana C Keselman, Rosemary M Lemons, Amanda H Mortensen, Marcelo A Marti, Sally A Camper, Jacob O Kitzman
BACKGROUND: Congenital Hypopituitarism is caused by genetic and environmental factors. Over 30 genes have been implicated in isolated and/or combined pituitary hormone deficiency. The etiology remains unknown for up to 80% of the patients, but most cases have been analyzed by limited candidate gene screening. Mutations in the PROP1 gene are the most common known cause, and the frequency of mutations in this gene varies greatly by ethnicity. We designed a custom array to assess the frequency of mutations in known hypopituitarism genes and new candidates, using single molecule molecular inversion probes sequencing (smMIPS)...
May 8, 2018: Molecular Genetics & Genomic Medicine
https://www.readbyqxmd.com/read/29738503/investigation-of-the-antifungal-activity-and-mode-of-action-of-thymus-vulgaris-citrus-limonum-pelargonium-graveolens-cinnamomum-cassia-ocimum-basilicum-and-eugenia-caryophyllus-essential-oils
#8
Katarzyna Gucwa, Sławomir Milewski, Tomasz Dymerski, Piotr Szweda
The antimicrobial activity of plant oils and extracts has been recognized for many years. In this study the activity of Thymus vulgaris , Citrus limonum , Pelargonium graveolens , Cinnamomum cassia , Ocimum basilicum , and Eugenia caryophyllus essential oils (EOs) distributed by Pollena Aroma (Nowy Dwór Mazowiecki, Poland) was investigated against a group of 183 clinical isolates of C. albicans and 76 isolates of C. glabrata . All of the oils exhibited both fungistatic and fungicidal activity toward C. albicans and C...
May 8, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29735577/hepatitis-a-virus-and-hepatitis-e-virus-emerging-and-re-emerging-enterically-transmitted-hepatitis-viruses
#9
Stanley M Lemon, Christopher M Walker
Over the past two decades, progress in understanding human infections with hepatitis A virus (HAV) and hepatitis E virus (HEV) has been eclipsed by the priority of combating persistent hepatitis B virus (HBV) and hepatitis C virus (HCV) infections. During that time, the global burden of liver disease caused by enteric hepatitis viruses has not abated. Because of vaccines, hepatitis A has become increasingly a disease of adults instead of early childhood in many regions of the world, resulting in an age-related shift toward more severe disease...
May 7, 2018: Cold Spring Harbor Perspectives in Medicine
https://www.readbyqxmd.com/read/29735576/enterically-transmitted-non-a-non-b-hepatitis-and-the-discovery-of-hepatitis-e-virus
#10
Stanley M Lemon, Christopher M Walker
The recognition of hepatitis E as a discreet disease entity in the late 1970s followed the development of serological tests for hepatitis A and the discovery that large waterborne outbreaks of hepatitis in India were not caused by hepatitis A virus (HAV). These "enterically transmitted non-A, non-B hepatitis" outbreaks had distinctive epidemiologic features, including the highest attack rates among young adults, little secondary household transmission of infection, and severe disease in pregnant women...
May 7, 2018: Cold Spring Harbor Perspectives in Medicine
https://www.readbyqxmd.com/read/29733893/applying-the-3rs-to-neuroscience-research-involving-nonhuman-primates
#11
Roger N Lemon
This Feature focuses on UK neuroscience research using nonhuman primates (NHPs), and the application of the 3Rs, in the light of the recent EU SCHEER report and subsequent article by Prescott et al. The challenge of understanding the human brain and its disorders means that NHP research is still very much needed, although it is essential that this research is complemented by studies using other approaches, such as human volunteers and patients, and other alternatives to NHP use. Analysis of recent publications shows that these complementary approaches are already being actively exploited by NHP researchers in the UK...
May 4, 2018: Drug Discovery Today
https://www.readbyqxmd.com/read/29733321/noninvasive-high-throughput-determination-of-sleep-duration-in-rodents
#12
R Michelle Saré, Abigail Lemons, Anita Torossian, Carolyn Beebe Smith
Traditionally, sleep is monitored by an electroencephalogram (EEG). EEG studies in rodents require surgical implantation of the electrodes followed by a long recovery period. To perform an EEG recording, the animal is connected to a receiver, creating an unnatural tether to the head-mount. EEG monitoring is time consuming, carries risk to the animal, and is not a completely natural setting for the measurement of sleep. Alternative methods to detect sleep, particularly in a high-throughput fashion, would greatly advance the field of sleep research...
April 18, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29731580/evaluation-of-biosafe-alternatives-as-xylene-substitutes-in-hematoxylin-and-eosin-staining-procedure-a-comparative-pilot-study
#13
Taneeru Sravya, Guttikonda Venkateswara Rao, Masabattula Geetha Kumari, Yerraguntla Vidya Sagar, Yeluri Sivaranjani, Kondamarri Sudheerkanth
Background: Xylene is synthetic hydrocarbon produced from coal tar known for its wide usage as universal solvent which has many hazardous effects. The aim of this study is to compare the efficacy of xylene-free hematoxylin and eosin (H and E) sections with conventional H and E sections. Materials and Methods: The study included ninety paraffin-embedded tissue blocks. Of these, sixty blocks were processed with sesame oil (xylene alternative) and thirty blocks with xylene...
January 2018: Journal of Oral and Maxillofacial Pathology: JOMFP
https://www.readbyqxmd.com/read/29729504/new-food-approaches-to-reduce-and-or-eliminate-increased-gastric-acidity-related-to-gastroesophageal-pathologies
#14
Ciro Langella, Daniele Naviglio, Marina Marino, Armando Calogero, Monica Gallo
OBJECTIVE: Gastroesophageal reflux disease is very common in industrialized countries and rapidly and significantly increasing even in developing countries. The approach in this study is one not commonly found to date in the scientific literature. To assess the ability of reduced-carbohydrate diets and foods that are enriched with acid potential of hydrogen (pH; lemon and tomato) to quickly and exponentially reduce symptoms that are related to conditions such as gastritis and gastroesophageal reflux and unrelated to Helicobacter pylori...
March 22, 2018: Nutrition
https://www.readbyqxmd.com/read/29729045/characterization-of-fungi-in-office-dust-comparing-results-of-microbial-secondary-metabolites-fungal-its-region-sequencing-viable-culture-and-other-microbial-indices
#15
Ju-Hyeong Park, Michael Sulyok, Angela R Lemons, Brett J Green, Jean M Cox-Ganser
Recent developments in molecular and chemical methods have enabled the analysis of fungal DNA and secondary metabolites, often produced during fungal growth, in environmental samples. We compared three fungal analytical methods by analyzing floor dust samples collected from an office building for fungi using viable culture, internal transcribed spacer (ITS) sequencing, and secondary metabolites using liquid chromatography-tandem mass spectrometry. Of the 32 metabolites identified, 29 had a potential link to fungi with levels ranging from 0...
May 4, 2018: Indoor Air
https://www.readbyqxmd.com/read/29726761/development-of-infectious-cdna-clones-of-citrus-yellow-vein-clearing-virus-using-a-novel-and-rapid-strategy
#16
Tian Tian Cui, Yu Bin, Jian Hong Yan, Peng Ying Mei, Zhong An Li, Chang Yong Zhou, Zhen Song
Yellow vein clearing disease (YVCD) causes significant economic losses in lemon and other species of citrus. Usually, citrus yellow vein clearing virus (CYVCV) is considered to be the causal agent of YVCD. However, mixed infection of CYVCV and Indian citrus ringspot virus (ICRSV) or other pathogens is often detected in citrus plants with YVCD. In this study, we re-examined the causal agent of YVCD to fulfill Koch's postulates. First, the full-length genome of CYVCV isolate AY (CYVCV-AY) was amplified by long-distance RT-PCR from a Eureka lemon [Citrus limon (L) Brum...
May 4, 2018: Phytopathology
https://www.readbyqxmd.com/read/29726671/the-ethics-of-bundled-payments-in-total-joint-replacement-cherry-picking-and-lemon-dropping
#17
Casey Jo Humbyrd
The Centers for Medicare & Medicaid Services has initiated bundled payments for hip and knee total joint replacement in an effort to decrease healthcare costs and increase quality of care. The ethical implications of this program have not been studied. This article considers the ethics of patient selection to improve outcomes; specifically, screening patients by body mass index to determine eligibility for total joint replacement. I argue that this type of screening is not ethically defensible, and that the bundled payment program as structured is likely to lead to unfair restrictions on who receives total joint replacements...
2018: Journal of Clinical Ethics
https://www.readbyqxmd.com/read/29712683/comparative-pathology-of-hepatitis-a-virus-and-hepatitis-e-virus-infection
#18
John M Cullen, Stanley M Lemon
Hepatitis A virus (HAV) and hepatitis E virus (HEV) cause acute, self-limiting hepatic infections that are usually spread by the fecal-oral route in humans. Naturally occurring and experimental infections are possible in a variety of nonhuman primates and, in the case of HEV, a number of other species. Many advances in understanding the pathogenesis of these viruses have come from studies in experimental animals. In general, animals infected with these viruses recapitulate the histologic lesions seen in infected humans, but typically with less severe clinical and histopathological manifestations...
April 30, 2018: Cold Spring Harbor Perspectives in Medicine
https://www.readbyqxmd.com/read/29703387/food-drug-interactions-precipitated-by-fruit-juices-other-than-grapefruit-juice-an-update-review
#19
REVIEW
Meng Chen, Shu-Yi Zhou, Erlinda Fabriaga, Pian-Hong Zhang, Quan Zhou
This review addressed drug interactions precipitated by fruit juices other than grapefruit juice based on randomized controlled trials (RCTs). Literature was identified by searching PubMed, Cochrane Library, Scopus and Web of Science till December 30 2017. Among 46 finally included RCTs, six RCTs simply addressed pharmacodynamic interactions and 33 RCTs studied pharmacokinetic interactions, whereas seven RCTs investigated both pharmacokinetic and pharmacodynamic interactions. Twenty-two juice-drug combinations showed potential clinical relevance...
April 2018: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/29698776/aloysia-citrodora-pal%C3%A3-u-lemon-verbena-a-review-of-phytochemistry-and-pharmacology
#20
REVIEW
Roodabeh Bahramsoltani, Pourouchista Rostamiasrabadi, Zahra Shahpiri, André M Marques, Roja Rahimi, Mohammad Hosein Farzaei
ETHNOPHARMACOLOGICAL RELEVANCE: Aloysia citrodora Paláu (Lippia citriodora Kunth), commonly known as "lemon verbena" is a medicinal plant native to South America, North Africa, and South of Europe which is used by native people for several indications such as diarrhea, flatulence, insomnia, and rheumatism. AIM OF THE REVIEW: Despite the wide biological activities of lemon verbena, there is no current review summarizing medicinal properties of the plant; thus, this paper aims to discuss current state of the art regarding the phytochemistry, pharmacology, and therapeutic applications of A...
April 23, 2018: Journal of Ethnopharmacology
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