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Packed RBC

Claudia Delgado-Corcoran, Katherine H Wolpert, Kathryn Lucas, Stephanie Bodily, Angela P Presson, Susan L Bratton
OBJECTIVE: To determine whether judicious blood testing impacts timing or amount of packed RBC transfusions in infants after heart surgery. DESIGN: A retrospective study comparing before and after initiation of a quality improvement process. SETTING: A university-affiliated cardiac ICU at a tertiary care children's hospital. PATIENTS: Infants less than 1 year old with Risk Adjustment for Congenital Heart Surgery category 4, 5, 6, or d-transposition of great arteries (Risk Adjustment for Congenital Heart Surgery 3) consecutively treated during 2010 through 2013...
September 22, 2016: Pediatric Critical Care Medicine
Eric Seibert, Anja Richter, Martin K Kuhlmann, Suxin Wang, Nathan W Levin, Peter Kotanko, Garry J Handelman
Introduction The achievement of erythropoiesis in hemodialysis (HD) patients is typically managed with erythropoiesis-stimulating-agents (ESA's) and intravenous iron (IV-iron). Using this treatment strategy, HD patients frequently show an elevated fraction of red blood cells (RBC) with hemoglobin (Hb) content per cell that is below the normal range, called hypochromic RBC. The low Hb content per RBC is the result of the clinical challenge of providing sufficient iron content to the bone marrow during erythropoiesis...
September 12, 2016: Hemodialysis International
K Sunil Raviraj, P Miglani, A Garg, P K Agarwal
Mucormycosis, is an emerging fungal infection in immunocompromised and diabetic individuals, usually affects rhino-orbito-cerebral, cutaneous and pulmonary regions. But mucormycosis in immunocompetent environment is rare and occurrence of gastric mucormycosis is unusual. We report a case of 19 year old female, with no pre-existing co-morbidities, presented with fever, dysentery, vomiting, and melena for 4 days. On evaluation she was found to have pancytopenia, acute kidney injury, hemolytic anemia, coagulopathy and hepatic derangement and treated with hemodialysis, plasmapheresis along with antibiotics and packed cell RBC transfusion...
October 2015: Journal of the Association of Physicians of India
Samuel B Berry, Syrena C Fernandes, Anjali Rajaratnam, Nicholas S DeChiara, Charles R Mace
The quantification of blood cells provides critical information about a patient's health status. Sophisticated analytical equipment, such as hematology analyzers, have been developed to perform these measurements, but limited-resource settings often lack the infrastructure required to purchase, operate, and maintain instrumentation. To address these practical challenges, paper-based microfluidic devices have emerged as a platform to develop diagnostic assays specifically for use at the point-of-care. To date, paper-based microfluidic devices have been used broadly in diagnostic assays that apply immunoassay, clinical chemistry, and electrochemistry techniques...
October 7, 2016: Lab on a Chip
Ronald Tesoriero, Brandon Bruns, Mayur Narayan, Joseph Dubose, Sundeep Guliani, Megan Brenner, Sharon Boswell, Deborah M Stein, Thomas Scalea
INTRODUCTION: Major pelvic disruption with hemorrhage has a high rate of lethality. Angiographic embolization remains the mainstay of treatment. Delays to angiography have been shown to worsen outcomes, in part because time spent awaiting mobilization of resources needed to perform angiography allows ongoing hemorrhage. Alternative techniques like pelvic pre-peritoneal packing (PPP) and aortic balloon occlusion (REBOA) now exist. We hypothesized that time to angiographic embolization at our level 1 trauma center would be longer than 90 minutes...
September 16, 2016: Journal of Trauma and Acute Care Surgery
Yiyi Cao, Li Yang, Nannan Feng, Oumin Shi, Jing Xi, Xinyue You, Chunrong Yin, Huan Yang, Katsuyoshi Horibata, Masamitsu Honma, Biyun Qian, Wei Weng, Yang Luan
Erythrocyte-based PIG-A assay is sensitive and reliable in detecting exposure to mutagenetic agents in animal studies, but there are few data from human populations. In this study, we employed a method for detecting CD59 phenotypic variants, resulting from mutation in the PIG-A gene, in human red blood cells (RBCs), and determined the CD59-deficient RBC (RBC(CD59-) ) frequencies in 217 subjects from general population. The majority of subjects had a relatively low mutant frequencies (MFs) (average, 5.25 ± 3...
October 2016: Environmental and Molecular Mutagenesis
Giuseppe Paglia, Angelo D'Alessandro, Óttar Rolfsson, Ólafur E Sigurjónsson, Aarash Bordbar, Sirus Palsson, Travis Nemkov, Kirk C Hansen, Sveinn Gudmundsson, Bernhard O Palsson
Metabolomic investigations of packed red blood cells (RBCs) stored under refrigerated conditions in saline adenine glucose mannitol (SAGM) additives have revealed the presence of 3 distinct metabolic phases, occurring on days 0-10, 10-18, and after day 18 of storage. Here we used receiving operating characteristics curve analysis to identify biomarkers that can differentiate between the 3 metabolic states. We first recruited 24 donors and analyzed 308 samples coming from RBC concentrates stored in SAGM and additive solution 3...
September 29, 2016: Blood
Jae Min Lee, Eun Sun Kim, Hoon Jai Chun, Young-Jae Hwang, Jae Hyung Lee, Seung Hun Kang, In Kyung Yoo, Seung Han Kim, Hyuk Soon Choi, Bora Keum, Yeon Seok Seo, Yoon Tae Jeen, Hong Sik Lee, Soon Ho Um, Chang Duck Kim
BACKGROUND AND STUDY AIMS: Many patients with acute gastrointestinal bleeding present with anemia and frequently require red blood cell (RBC) transfusion. A restrictive transfusion strategy and a low hemoglobin (Hb) threshold for transfusion had been shown to produce acceptable outcomes in patients with acute upper gastrointestinal bleeding. However, most patients are discharged with mild anemia owing to the restricted volume of packed RBCs (pRBCs). We investigated whether discharge Hb influences the outcome in patients with acute nonvariceal upper gastrointestinal bleeding...
August 2016: Endoscopy International Open
G Thirumalaisamy, M R Purushothaman, P Vasantha Kumar, P Selvaraj
AIM: The study was undertaken to find out the effect of feeding cottonseed meal (CSM) on performance of hematological and serum biochemical parameters in broiler birds. MATERIALS AND METHODS: A 6-week biological trial was carried out with 432-day-old Cobb 400 broiler chicks distributed to nine experimental diets with six replicates, each containing eight chicks. The experimental diets were formulated based on total amino acids (BTAA) or based on digestible amino acids (BDAA) with or without iron supplementation with two levels of CSM (2% and 4%) and control diet based on maize - soybean...
July 2016: Veterinary World
Charlotte Cuerq, Lioara Restier, Jocelyne Drai, Emilie Blond, Adeline Roux, Sybil Charriere, Marie-Caroline Michalski, Mathilde Di Filippo, Emile Levy, Alain Lachaux, Noël Peretti
BACKGROUND: Chylomicron retention disease (CMRD), a rare genetic hypocholesterolemia, results in neuro-ophtalmologic damages, which can be prevented by high doses of vitamin E during infancy. In these patients, plasma vitamin E concentration is significantly reduced due to defects of chylomicron secretion. Vitamin E in adipose tissue (AT) and red blood cells (RBC) have been proposed as potential relevant biomarkers of vitamin E status but no reference values in children are available...
2016: Orphanet Journal of Rare Diseases
Nursakinah Suardi, Bashiru Kayode Sodipo, Mohd Zulkifli Mustafa, Zalila Ali
In this work we present influence of visible laser light on ATP level and viability of anaemic red blood cell (RBC). The visible laser lights used in this work are 460nm and 532nm. The responses of ATP level in anaemic and normal RBC before and after laser irradiation at different exposure time (30, 40, 50 and 60s) were observed. Three aliquots were prepared from the ethylenediaminetetraacetic acid (EDTA) blood sample. One served as a control (untreated) and another two were irradiated with 460nm and 560nm lasers...
September 2016: Journal of Photochemistry and Photobiology. B, Biology
Kent R Zettel, Mitchell Dyer, Jay S Raval, Xubo Wu, John R Klune, Andres Gutierrez, Darrell J Triulzi, Timothy R Billiar, Matthew D Neal
Red blood cell transfusions in the setting of trauma is a double edged sword, as it is a necessary component for life-sustaining treatment in massive hemorrhagic shock, but also associated with increased risk for nosocomial infections and immune suppression. The mechanisms surrounding this immune suppression are unclear. Using supernatant from human packed red blood cell (RBC), we demonstrate that clearance of E. coli by macrophages is inhibited both in vitro and in vivo using a murine model of trauma and hemorrhagic shock...
August 2, 2016: Shock
D Aktaran Bala, M Özcan
Human medicine studies have so far demonstrated that erythrocytes may be preserved and stored at low temperatures for decades retaining their metabolic and biochemical properties. However, detailed studies regarding this problem are not yet available in veterinary medicine. Therefore, the objective of the current study was to investigate time-dependent effects of long-term frozen storage of canine red blood cells. Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged by a cryogenic microcentrifuge and packed RBC suspensions were obtained...
2016: Polish Journal of Veterinary Sciences
M P Martínez, M I Conti, C E Lezón, R M Alippi, C E Bozzini
The recent report of a depression of stimulated production of erythropoietin (EPO) in mice with enhanced erythropoiesis suggests that unknown mechanism (s) other than hypoxia may be involved in the regulation of EPO formation. The present study was thus designed to investigate EPO production during acute hypoxemia in a mouse model in which the oxygen-carrying capacity of blood, the plasma EPO level, and the plasma EPO half-life were within normal values in spite of a marked depression of the red cell production rate (RCPR) induced by cyclophosphamide (CP) administration...
1996: Hematology (Amsterdam, Netherlands)
Edwin C Y Chow, Jason Z Ya Wang, Holly P Quach, Hui Tang, David C Evans, Albert P Li, Jose Silva, K Sandy Pang
Chimeric mouse liver models are useful in vivo tools for human drug metabolism studies; however, liver integrity and the microcirculation remain largely uninvestigated. Hence, we conducted liver perfusion studies to examine these attributes in FRGN [Fah(-/-), Rag2(-/-), and Il2rg(-/-), NOD strain] livers (control) and chimeric livers repopulated with mouse (mFRGN) or human (hFRGN) hepatocytes. In single-pass perfusion studies (2.5 ml/min), outflow dilution profiles of noneliminated reference indicators ((51)Cr-RBC, (125)I-albumin, (14)C-sucrose, and (3)H-water) revealed preservation of flow-limited distribution and reduced water and albumin spaces in hFRGN livers compared with FRGN livers, a view supported microscopically by tightly packed sinusoids...
September 2016: Drug Metabolism and Disposition: the Biological Fate of Chemicals
Anil Gattani, Arti Pathak, Ajeet Kumar, Vaibhav Mishra, Jitendra Singh Bhatia
AIM: The objective of this study was to evaluate the effect of season and sex on hemato-biochemical parameters of turkey (Meleagris gallopavo) in the arid tropical environment. MATERIALS AND METHODS: The experiment was conducted on 20-week old turkeys consisting of 20 males and 20 females. Blood was collected from all turkeys during January and May. Hemoglobin (Hb), red blood cell (RBC), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were estimated in whole blood and glucose, protein, albumin, globulin, A/G ratio, calcium, phosphorus, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in serum...
May 2016: Veterinary World
T G Poder, D Pruneau, J Dorval, L Thibault, J-F Fisette, S K Bédard, A Jacques, P Beauregard
BACKGROUND AND OBJECTIVES: Fluid warmers are routinely used to reduce the risk of hypothermia and cardiac complications associated with the infusion of cold blood products. However, warming blood products could generate haemolysis. This study was undertaken to compare the impact of temperature of blood warmers on the per cent haemolysis of packed red blood cells (RBCs) heated at different flow rates as well as non-flow conditions. MATERIALS AND METHODS: Infusion warmers used were calibrated at 41·5°C ± 0·5°C and 37·5°C ± 0·5°C...
June 9, 2016: Vox Sanguinis
Iver Anders Gaski, Jeppe Barckman, Paal Aksel Naess, Nils Oddvar Skaga, Jan Erik Madsen, Nils Einar Kløw, Gunnar Flugsrud, Christine Gaarder
BACKGROUND: Extraperitoneal pelvic packing (EPP) was introduced at Oslo University Hospital Ulleval (OUHU) in 1994. Published studies from other institutions have advocated its application as a first-line therapy in lieu of angiography. Extraperitoneal pelvic packing is invasive with a high risk of complications, and its role remains an issue of discussion. In line with international trends, an updated massive hemorrhage protocol was implemented at OUHU in 2007. We hypothesized a decreased need for EPP owing to the major changes in resuscitation strategies...
October 2016: Journal of Trauma and Acute Care Surgery
Philippe Chadebech, Marie-Amélie de Ménorval, Gwellaouen Bodivit, Armand Mekontso-Dessap, Sadaf Pakdaman, Alicia Jouard, Frédéric Galactéros, Philippe Bierling, Anoosha Habibi, France Pirenne
BACKGROUND: The transfusion of red blood cell (RBC) concentrates is the main treatment for acute vaso-occlusive symptoms in sickle cell disease (SCD). Units of packed RBCs (pRBCs) must retain optimal characteristics for transfusion throughout the storage period. Transfused RBCs interact with the plasma and the endothelium that lines blood vessels and may be the target of immune-hematologic conflict if the patient produces antibodies against RBCs. Questions remain concerning the benefit-risk balance of RBC transfusions, in particular about the shelf-life of the units...
July 2016: Transfusion
Erden Atilla, Selami Koçak Toprak, Sinem Civriz Bozdağ, Pervin Topçuoğlu, Önder Arslan
OBJECTIVE: The hemoglobin (Hb) content of the packed red blood cell (pRBC) differs in standart volume unit. The packed red pRBC transfusions are based on the number of units routinely. We aimed to use pRBCs according to total hemoglobin (Hb) content and compare the rates of achieving the target Hb concentration levels with the current transfusion practice. MATERIALS AND METHODS: 89 patients (55M; 34F) with median age 46 (range, 19-75) were enrolled and from 178 transfusion episodes 92 were randomized to Hb content based- study group and 86 to unit based- control group...
February 20, 2015: Turkish Journal of Haematology: Official Journal of Turkish Society of Haematology
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