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https://www.readbyqxmd.com/read/29341028/ameliorative-effects-of-zataria-multiflora-hydro-alcoholic-extract-on-gentamicin-induced-nephrotoxicity-in-rats
#1
Saeed Hajihashemi, Tahereh Jafarian, Mahboubeh Ahmadi, Ali Rahbari, Fatemeh Ghanbari
BACKGROUND: Nephrotoxicity is the major side effects of aminoglycoside antibiotics such as gentamicin. The aim of this study was to investigate the therapeutic effects of Z.multiflora (ZM) hydroalcoholic extract on gentamicin-induced nephrotoxicity. METHODS: Twenty-eight male Wistar rats categorized into four groups: 1) the control group without injection, 2) the gentamicin treated group (100 mg/kg, i.p) 3) the gentamicin and Z. Multiflora treated group; Z. Multiflora extract(0...
January 16, 2018: Drug Research
https://www.readbyqxmd.com/read/29338760/increased-plasmodium-chabaudi-malaria-mortality-in-mice-with-nutritional-iron-deficiency-can-be-reduced-by-short-term-adjunctive-iron-supplementation
#2
Filip C Castberg, Lasse Maretty, Trine Staalsoe, Casper Hempel, Erik Clasen-Linde, Lars Hviid, Jørgen A L Kurtzhals
BACKGROUND: Iron deficiency is the most widespread nutrient deficiency and an important cause of developmental impairment in children. However, some studies have indicated that iron deficiency can also protect against malaria, which is a leading cause of childhood morbidity and mortality in large parts of the world. This has rendered interventions against iron deficiency in malaria-endemic areas controversial. METHODS: The effect of nutritional iron deficiency on the clinical outcome of Plasmodium chabaudi AS infection in A/J mice and the impact of intravenous iron supplementation with ferric carboxymaltose were studied before and after parasite infection...
January 16, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29338257/a-dynamic-protein-protein-coupling-between-the-tonb-dependent-transporter-fhua-and-tonb
#3
Jessica Sarver, Michael Zhang, Lishan Liu, David Nyenhuis, David S Cafiso
Bacterial outer membrane TonB-dependent transporters function by executing cycles of binding and unbinding to the inner membrane protein TonB. In the vitamin B12 transporter BtuB and the ferric citrate transporter FecA, substrate binding increases the periplasmic exposure of the Ton box, an energy-coupling segment. This increased exposure appears to enhance the affinity of the transporter for TonB. Here, continuous wave and pulse EPR spectroscopy were used to examine the state of the Ton box in the Escherichia coli ferrichrome transporter, FhuA...
January 17, 2018: Biochemistry
https://www.readbyqxmd.com/read/29336556/linking-spectral-induced-polarization-sip-and-subsurface-microbial-processes-results-from-sand-column-incubation-experiments
#4
Adrian Mellage, Christina M Smeaton, Alex Furman, Estella Atekwana, Fereidoun Rezanezhad, Philippe Van Cappellen
Geophysical techniques, such as Spectral Induced Polarization (SIP), offer potentially powerful approaches for in-situ monitoring of subsurface biogeochemistry. The successful implementation of these techniques as monitoring tools for reactive transport phenomena, however, requires the de-convolution of multiple contributions to measured signals. Here, we present SIP spectra and complementary biogeochemical data obtained in saturated columns packed with alternating layers of ferrihydrite-coated and pure quartz sand, and inoculated with Shewanella oneidensis supplemented with lactate and nitrate...
January 16, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29334382/a-de-novo-enzyme-catalyzes-a-life-sustaining-reaction-in-escherichia-coli
#5
Ann E Donnelly, Grant S Murphy, Katherine M Digianantonio, Michael H Hecht
Producing novel enzymes that are catalytically active in vitro and biologically functional in vivo is a key goal of synthetic biology. Here we describe Syn-F4, the first de novo protein that meets both criteria. Purified Syn-F4 hydrolyzes the siderophore ferric enterobactin, and expression of Syn-F4 allows an inviable strain of Escherichia coli to grow in iron-limited medium. These findings demonstrate that entirely new sequences can provide life-sustaining enzymatic functions in living organisms.
January 15, 2018: Nature Chemical Biology
https://www.readbyqxmd.com/read/29333856/analysis-of-the-ph-dependent-fe-iii-ion-chelating-activity-of-anthocyanin-extracted-from-black-soybean-glycine-max-l-merr-coats
#6
Yanli Xie, Xiaolu Zhu, Yuan Li, Cheng Wang
The Fe(III) chelating activity of anthocyanin extracted from black soybean coats was investigated at pH 3.0, 5.0, 6.5, 7.0 and 7.4 with fluorescence spectroscopy and microscale thermophoresis (MST). Cyanidin-3-glucoside (C3G) was determined to be 98% of the total anthocyanin by HPLC. The binding affinity (Ka) exhibited significant pH-dependent behavior; Ka was 9.7167, 1.0837, 1.4284, 5.4550 and 3.0269×104 M-1 at pH 3.0, 5.0, 6.5, 7.0 and 7.4, respectively (p<0.05). The MST data showed that ΔG<0 and ΔH<0, demonstrating that chelation is spontaneous and exothermic...
January 15, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29333391/analysis-of-antioxidant-and-anti-inflammatory-activities-of-solvent-fractions-from-rhynchosia-nulubilis-cultivated-with-ganoderma-lucidum-mycelium
#7
Mihye Park, Meera Kim
In this study, the crude ethanol Rhynchosia nulubilis cultivated with Ganoderma lucidum mycelium (RNGM) extract was solvent fractionated with organic solvents such as n-hexane, chloroform, ethyl acetate, and water. The anticancer activities, anti-inflammatory activity total polyphenols, total flavonoids, isoflavones, and β-glucan of the solvent fractions of RNGM were studied. The ethyl acetate fraction showed the highest 2,2-diphenyl-1-picrylhydrazyl scavenging activity of 76.60% at 800 μg/mL. The ethyl acetate fraction also showed higher antioxidant activity in 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and ferric reducing ability of plasma assays compared to the other fractions...
December 2017: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/29333388/enhancement-of-phenolic-production-and-antioxidant-activity-from-buckwheat-leaves-by-subcritical-water-extraction
#8
Dong-Shin Kim, Mi-Bo Kim, Sang-Bin Lim
To enhance the production of phenolic compounds with high antioxidant activity and reduce the level of phototoxic fagopyrin, buckwheat leaves were extracted with subcritical water (SW) at 100~220°C for 10~50 min. The major phenolic compounds were quercetin, gallic acid, and protocatechuic acid. The cumulative amount of individual phenolic compounds increased with increasing extraction temperature from 100°C to 180°C and did not change significantly at 200°C and 220°C. The highest yield of individual phenolic compounds was 1,632...
December 2017: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/29333386/antioxidant-and-anti-adipogenic-activities-of-trapa-japonica-shell-extract-cultivated-in-korea
#9
DooJin Lee, Ok-Hwan Lee, Geunpyo Choi, Jong Dai Kim
Trapa japonica shell contains phenolic compounds such as tannins. Studies regarding the antioxidant and anti-adipogenic effects of Trapa japonica shell cultivated in Korea are still unclear. Antioxidant and anti-adipogenic activities were measured by in vitro assays such as 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging activity, 2,2'-azinobis( 3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity, ferric reducing ability of plasma assay, reducing power, superoxide dismutase-like activity, and iron chelating ability in 3T3-L1 cells...
December 2017: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/29331810/behavior-of-cylindrospermopsis-raciborskii-during-coagulation-and-sludge-storage-higher-potential-risk-of-toxin-release-than-microcystis-aeruginosa
#10
Hongmin Li, Haiyan Pei, Hangzhou Xu, Yan Jin, Jiongming Sun
Owing to the global warming and its strong adaptability, Cylindrospermopsis raciborskii has spread world-wide. However, as one of toxic cyanobacteria in many drinking water sources, it has not been drawn proper consideration in drinking water treatment plants so far. The investigation aimed at unveiling the fate of C. raciborskii during polyaluminum ferric chloride (PAFC) coagulation and sludge storage, revealing its differences from Microcystis aeruginosa. Results showed that C. raciborskii cells were effectively removed intactly under optimum coagulation conditions, but PAFC at higher dosages (>10 mg/L) triggered additional cylindrospermopsins release...
January 5, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29330649/growth-of-leptospirillum-ferriphilum-in-sulfur-medium-in-co-culture-with-acidithiobacillus-caldus
#11
Sarah L Smith, D Barrie Johnson
Leptospirillum ferriphilum and Acidithiobacillus caldus are both thermotolerant acidophilic bacteria that frequently co-exist in natural and man-made environments, such as biomining sites. Both are aerobic chemolithotrophs; L. ferriphilum is known only to use ferrous iron as electron donor, while A. caldus can use zero-valent and reduced sulfur, and also hydrogen, as electron donors. It has recently been demonstrated that A. caldus reduces ferric iron to ferrous when grown aerobically on sulfur. Experiments were carried out which demonstrated that this allowed L...
January 12, 2018: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29329289/burkholderia-pseudomallei-modulates-host-iron-homeostasis-to-facilitate-iron-availability-and-intracellular-survival
#12
Imke H E Schmidt, Claudia Gildhorn, Martha A L Böning, Vera A Kulow, Ivo Steinmetz, Antje Bast
BACKGROUND: The control over iron homeostasis is critical in host-pathogen-interaction. Iron plays not only multiple roles for bacterial growth and pathogenicity, but also for modulation of innate immune responses. Hepcidin is a key regulator of host iron metabolism triggering degradation of the iron exporter ferroportin. Although iron overload in humans is known to increase susceptibility to Burkholderia pseudomallei, it is unclear how the pathogen competes with the host for the metal during infection...
January 12, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29328981/a-simple-lc-ms-ms-method-for-determination-of-deferasirox-in-human-plasma-troubleshooting-of-interference-from-ferric-ion-in-method-development-and-its-application
#13
Tengfei Li, Zhimin Cui, Yan Wang, Wen Yang, Duo Li, QinXin Song, Luning Sun, Li Ding
As an orally active iron chelator, deferasirox forms its ion complexes in the prepared plasma samples and LC-MS mobile phase where ferric ion exists, and then comparing with the nominal concentration level, a lower detected concentration level of deferasirox would be obtained after LC-MS analysis, if no proper treatment was adopted. Meanwhile, the phenomenon would be observed that multiple repeat injections of the same deferasirox plasma sample in the same tube would show the lower and lower detected concentration levels of deferasirox, which caused by more and more ferric ions from the injection needle dissolved in the sample solution as multiple repeated injections...
December 28, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29324685/novel-spectroscopic-and-electrochemical-sensors-and-nanoprobes-for-the-characterization-of-food-and-biological-antioxidants
#14
REVIEW
Reşat Apak, Sema Demirci Çekiç, Ayşem Üzer, Saliha Esin Çelik, Mustafa Bener, Burcu Bekdeşer, Ziya Can, Şener Sağlam, Ayşe Nur Önem, Erol Erçağ
Since an unbalanced excess of reactive oxygen/nitrogen species (ROS/RNS) causes various diseases, determination of antioxidants that can counter oxidative stress is important in food and biological analyses. Optical/electrochemical nanosensors have attracted attention in antioxidant activity (AOA) assessment because of their increased sensitivity and selectivity. Optical sensors offer advantages such as low cost, flexibility, remote control, speed, miniaturization and on-site/in situ analysis. Electrochemical sensors using noble metal nanoparticles on modified electrodes better catalyze bioelectrochemical reactions...
January 11, 2018: Sensors
https://www.readbyqxmd.com/read/29324642/antibacterial-and-antioxidant-capacities-and-attenuation-of-lipid-accumulation-in-3t3-l1-adipocytes-by-low-molecular-weight-fucoidans-prepared-from-compressional-puffing-pretreated-sargassum-crassifolium
#15
Chun-Yung Huang, Chia-Hung Kuo, Chia-Hsin Lee
In this study, we extracted fucoidan from compressional-puffing-pretreated Sargassum crassifolium by hot water. The crude extract of fucoidan (SC) was degraded by various degradation reagents and four low-molecular-weight (LMW) fucoidans, namely SCO (degradation by hydrogen peroxide), SCA (degradation by ascorbic acid), SCOA (degradation by hydrogen peroxide + ascorbic acid), and SCH (degradation by hydrogen chloride) were obtained. The degradation reagents studied could effectively degrade fucoidan into LMW fucoidans, as revealed by intrinsic viscosity, agarose gel electrophoresis, and molecular weight analyses...
January 11, 2018: Marine Drugs
https://www.readbyqxmd.com/read/29320163/reaction-intermediates-of-nitric-oxide-synthase-from-deinococcus-radiodurans-as-revealed-by-pulse-radiolysis-evidence-for-intramo-lecular-electron-transfer-from-biopterin-to-feii-o2-complex
#16
Yuko Tsutsui, Kazuo Kobayashi, Fusako Takeuchi, Motonari Tsubaki, Takahiro Kozawa
Nitric oxide synthase (NOS) is a cytochrome P450-type mono-oxygenase that catalyzes the oxidation of L-arginine (Arg) to nitric oxide (NO) through a reaction intermediate N-hydroxy-L-arginine (NHA). The mechanism underlying the reaction catalyzed by NOS from Deinococcus radiodurans was investigated using pulse radiolysis. Radiolytically-generated hydrated electrons reduced the heme iron of NOS within 2 μs. Subsequently, ferrous heme reacted with O2 to form a ferrous-dioxygen intermediated with a second-order rate constant of 2...
January 10, 2018: Biochemistry
https://www.readbyqxmd.com/read/29319418/aluminum-and-iron-leaching-from-power-plant-coal-fly-ash-for-preparation-of-polymeric-aluminum-ferric-chloride
#17
Yadong Zhang, Ming Li, Dong Liu, Xulian Hou, Jinglun Zou, Xiaotong Ma, Fanyi Shang, Zhaowen Wang
In this work, aluminum and iron existing in coal fly ash were extracted by method of hydrochloric acid leaching. Effects of solid-liquid ratio, reaction temperature, reaction time, acid concentration, and raw ash mesh on recovery efficiencies of Al2O3 and Fe2O3 were investigated. XRD analysis indicated that anhydrite, hematite, mullite and quartz were the dominant minerals in the raw fly ash sample. XRF technique was applied to determine the mass fractions of chemical components in the raw ash and leached residues, while the concentrations of Al2O3 and Fe2O3 in leaching solutions were measured by titration method...
January 10, 2018: Environmental Technology
https://www.readbyqxmd.com/read/29319001/vertically-grown-zinc-oxide-nanorods-functionalized-with-ferric-oxide-for-in-vivo-and-non-enzymatic-glucose-detection
#18
Mohammed Marie, Anishkumar Manoharan, Andrian V Kuchuk, Simon Ang, M Omar Manasreh
An enzyme-free glucose sensor out of vertically grown Zinc oxide nanorods (ZnO NRs) functionalized with ferric oxide Fe2O3 is investigated. The well-aligned and high-dense ZnO NRs were synthesized on an FTO/glass substrate by sol-gel and hydrothermal growth methods. Dip-coating technique was utilized to modify the surface of the as-grown ZnO NRs with Fe2O3, and the immobilized surface was coated with a layer of a nafion membrane. The fabricated glucose sensor was characterized amperometrically using three electrodes station at room temperature in the phosphate buffer solution, where ZnO NRs/Fe2O3/nafion membrane was the sensing or working electrode, platinum plate and silver/silver chloride were the counter and the reference electrodes respectively...
January 10, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29315586/dietary-enzymatically-treated-artemisia-annua-l-improves-meat-quality-antioxidant-capacity-and-energy-status-of-breast-muscle-in-heat-stressed-broilers
#19
Xiaoli Wan, Hussain Ahmad, Lili Zhang, Zhiyue Wang, Tian Wang
BACKGROUND: Heat stress (HS) is detrimental to animal origin food production. Artemisia annua L., a natural source of phenolic compounds and flavonoids, exhibits antioxidant property. This study was conducted to evaluate the effects of dietary enzymatically treated Artemisia annua L. (EA) supplementation on meat quality, antioxidant capacity and energy status of breast muscle in heat-stressed broilers. RESULTS: The EA inclusion increased the redness value, reduced drip loss, decreased reactive oxygen metabolites and thiobarbituric acid-reactive substances, increased antioxidant enzyme activities, and reduced the ferric reducing antioxidant power and free radical scavenging abilities of breast muscle in heat-treated broilers...
January 9, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29310631/acute-effect-of-clitoria-ternatea-flower-beverage-on-glycemic-response-and-antioxidant-capacity-in-healthy-subjects-a-randomized-crossover-trial
#20
Charoonsri Chusak, Thavaree Thilavech, Christiani Jeyakumar Henry, Sirichai Adisakwattana
BACKGROUND: Clitoria ternatea L., a natural food-colorant containing anthocyanin, demonstrated antioxidant and antihyperglycemic activity. The aim of this study was to determine the effects of Clitoria ternatea flower extract (CTE) on postprandial plasma glycemia response and antioxidant status in healthy men. METHODS: In a randomized, crossover study, 15 healthy men (ages 22.53 ± 0.30 years; with body mass index of 21.57 ± 0.54 kg/m2) consumed five beverages: (1) 50 g sucrose in 400 mL water; (2) 1 g CTE in 400 mL of water; (3) 2 g CTE in 400 mL of water; (4) 50 g sucrose and 1 g CTE in 400 mL of water; and (5) 50 g sucrose and 2 g CTE in 400 mL of water...
January 8, 2018: BMC Complementary and Alternative Medicine
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