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GLUT expression mononuclear cells

Elham Rahmani, Mehri Jamilian, Bahareh Dadpour, Zahra Nezami, Zahra Vahedpoor, Samaneh Mahmoodi, Esmat Aghadavod, Mohsen Taghizadeh, Abolfazl Beiki Hassan, Zatollah Asemi
BACKGROUND: This study was conducted to determine the effects of fish oil administration on gene expression related to insulin, lipid and inflammation in women with polycystic ovary syndrome (PCOS). MATERIALS AND METHODS: This randomized, double-blind, placebo-controlled trial was conducted among 40 subjects with PCOS, aged 18-40 years. Subjects were randomly allocated into two groups to take either 1000 mg omega-3 fatty acids from fish oil (n = 20) or placebo (n = 20) twice a day for 12 weeks...
March 2018: European Journal of Clinical Investigation
Mehri Jamilian, Azadeh Shojaei, Mansooreh Samimi, Faraneh Afshar Ebrahimi, Esmat Aghadavod, Maryam Karamali, Mohsen Taghizadeh, Hamidreza Jamilian, Somayeh Alaeinasab, Sadegh Jafarnejad, Zatollah Asemi
OBJECTIVE: The aim of this study was to evaluate the effects of omega-3 and vitamin E co-supplementation on parameters of mental health and gene expression related to insulin and inflammation in subjects with polycystic ovary syndrome (PCOS). METHODS: Forty PCOS women were allocated into two groups and treated with 1000mg omega-3 fatty acids plus 400 IU vitamin E supplements (n = 20) or placebo (n = 20) per day for 12 weeks. Parameters of mental health were recorded at baseline and after the 12-week intervention...
March 15, 2018: Journal of Affective Disorders
Gaia Spinetti, Elena Sangalli, Claudia Specchia, Francesco Villa, Chiara Spinelli, Rita Pipolo, Albino Carrizzo, Simona Greco, Christine Voellenkle, Carmine Vecchione, Paolo Madeddu, Fabio Martelli, Annibale Alessandro Puca
The study of the health status in long-living individuals (LLIs) may help identifying health-span and life-span determinants. BPI-Fold-Containing-Family-B-Member-4 (BPIFB4) protein is higher in healthy vs. non-healthy (frail) LLIs serum and its longevity-associated variant forced expression improves cardiovascular outcomes in ischemia mice models. Thus, we tested the association of BPIFB4 and ischemia-responding HIF-1α pathway components (i.e. CXCR4 , AK3, ALDO-C, ADM , VEGF-A , GLUT-1 and miR-210) with human life-span and health-span by analyzing mRNA expression in circulating mononuclear cells (MNCs) of LLIs (N=14 healthy; N=31 frail) and young controls (N=63)...
January 22, 2017: Aging
Britta Stapel, Alexandra Kotsiari, Michaela Scherr, Denise Hilfiker-Kleiner, Stefan Bleich, Helge Frieling, Kai G Kahl
The use of antipsychotics carries the risk of metabolic side effects, such as weight gain and new onset type-2 diabetes mellitus. The mechanisms of the observed metabolic alterations are not fully understood. We compared the effects of two atypical antipsychotics, one known to favor weight gain (olanzapine), the other not (aripiprazole), on glucose metabolism. Primary human peripheral blood mononuclear cells (PBMC) were isolated and stimulated with olanzapine or aripiprazole for 72 h. Cellular glucose uptake was analyzed in vitro by 18F-FDG uptake...
May 2017: Journal of Psychiatric Research
Elena Lima-Cabello, Victor Alche, Rhonda C Foley, Sofianos Andrikopoulos, Grant Morahan, Karam B Singh, Juan D Alche, Jose C Jimenez-Lopez
SCOPE: We have investigated the potential use of β-conglutin protein isoforms from narrow-leafed lupin (Lupinus angustifolius L.) as a diabetes treatment. METHODS AND RESULTS: We produced purified recombinant β1-, β2-, β3-, β4-, and β6-conglutin proteins and showed that β1, β3, and β6 could bind to insulin. To assess β-conglutin proteins modulatory effect on insulin activation meditated kinases, whole blood and peripheral blood mononuclear cell cultures from type 2 diabetes (T2D) and healthy control subjects (C) were incubated with conglutin proteins...
May 2017: Molecular Nutrition & Food Research
Carolina De Ciuceis, Vincenzo Flati, Claudia Rossini, Anna Rufo, Enzo Porteri, Jacopo Di Gregorio, Beatrice Petroboni, Elisa La Boria, Carlotta Donini, Evasio Pasini, Enrico Agabiti Rosei, Damiano Rizzoni
It was previously demonstrated that metabolic syndrome in humans is associated with an impairment of insulin signalling in circulating mononuclear cells. At least in animal models of hypertension, angiotensin-converting enzyme (ACE) inhibitors and angiotensin receptor blockers (ARB) may correct alterations of insulin signalling in the skeletal muscle. In the first study, we investigated the effects of a 3-month treatment with an ARB with additional PPARγ agonist activity, telmisartan, or with a dihydropyridine calcium channel blocker, nifedipine, on insulin signalling in patients with mild-moderate essential hypertension...
December 2014: Blood Pressure
N J O'Boyle, G A Contreras, S A Mattmiller, L M Sordillo
The transition period of dairy cows is characterized by dramatic changes in metabolism and immune cell function that contributes to increased susceptibility to several economically important diseases. Monocyte and macrophage populations increase in blood and tissues of cows during the transition period and have enhanced inflammatory responses that may contribute to increased severity of disease. Glucose is a major energy source for activated monocytes and glucose uptake is facilitated by glucose transporters (GLUT)...
October 2012: Journal of Dairy Science
Pham Van Phuc, Truong Hai Nhung, Dang Thi Tung Loan, Doan Chinh Chung, Phan Kim Ngoc
Umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) are multipotent cells. They are able to differentiate into functional cells from not only mesoderm but also endoderm. Many researches showed that cells derived from fresh human UCB could transdifferentiate into insulin-secreting cells. In this study, transdifferentiating potential of cryopreserved human UCB-derived MSCs into insulin-secreting cell was investigated. Fresh human UCB was enriched the mononuclear cells by Ficoll-Paque centrifugation...
January 2011: In Vitro Cellular & Developmental Biology. Animal
H J Knowles, N A Athanasou
Hypoxia is an important regulator of bone biology and stimulates osteoclast differentiation from monocytic precursors. Hypoxia-inducible factor (HIF) is a key pro-tumourigenic transcription factor mediating pathways of hypoxia-inducible gene expression. We have described expression of HIF-1alpha and HIF-2alpha in the multi-nucleated, osteoclast-like giant cells and the mononuclear stromal component of giant cell tumour of bone (GCTB), a locally osteolytic primary bone tumour. HIF induction was observed in culture in the osteoblastic MG-63 cell line, primary GCTB stromal cells, and monocyte-derived osteoclasts following stimulation with hypoxia (0...
May 2008: Journal of Pathology
Juan T Borda, Xavier Alvarez, Mahesh Mohan, Marion S Ratterree, Kathrine Phillippi-Falkenstein, Andrew A Lackner, Bruce A Bunnell
Globoid cell leukodystrophy, or Krabbe's disease, is a severe disorder of the central and peripheral nervous system caused by the absence of galactocerebrosidase (GALC) activity. Herein, we describe the clinical, neuropathological, histochemical, and immunohistological features observed in rhesus macaques affected with Krabbe's disease. Clinical signs included pronounced muscle tremors of head and limbs, difficulty ambulating, ataxia, hypermetria, proprioceptive deficits, and respiratory abnormalities. Histopathologically, all animals presented with evidence of demyelination in the peripheral and central nervous systems and accumulation of mononuclear and multinuclear globoid cells in the cerebral and cerebellar white matter associated with severe gliosis...
January 2008: American Journal of Pathology
J Rasschaert, D Liu, B Kutlu, A K Cardozo, M Kruhøffer, T F ØRntoft, D L Eizirik
AIMS/HYPOTHESIS: Viral infections and local production of IFN-gamma might contribute to beta-cell dysfunction/death in Type 1 Diabetes. Double stranded RNA (dsRNA) accumulates in the cytosol of viral-infected cells, and exposure of purified rat beta cells to dsRNA (tested in the form of polyinosinic-polycytidylic acid, PIC) in combination with IFN-gamma results in beta-cell dysfunction and apoptosis. To elucidate the molecular mechanisms involved in PIC + IFN-gamma-effects, we determined the global profile of genes modified by these agents in primary rat beta cells...
December 2003: Diabetologia
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