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Dongdong Zhao, Zhihui Zhou, Xiaoting Hua, Huichuan Zhang, Jingjing Quan, Xi Li, Yunsong Yu
Here we report the first clinical Escherichia coli isolate co-harboring mcr-1, blaKPC-2 and two copies of fosA3 from China. The five plasmids of the isolate were completely sequenced and analyzed. Gene mcr-1 and blaKPC-2 were located on IncI2 and IncR plasmid, respectively. A variety of other resistance determinants such as fosA3 (two copies), blaCTX-M-123 , blaOXA-1 and blaCTX-M-65 were also identified from the rest plasmids.
February 19, 2018: Infection, Genetics and Evolution
Mingjian Bai, Jing Feng, Guowei Liang
OBJECTIVE: We want to determine whether urinary myeloperoxidase to creatinine ratio could be served as a new marker for monitoring treatment effects of urinary tract infection or not. METHODS: A total of 328 patients suspected of UTI were enrolled in present study. Patients been received antibiotic therapy within two weeks were excluded (n = 26). Patients with urine contaminated specimens (n = 49) and negative urine culture results (n = 96) were also excluded, the remaining culture positive subjects (n = 157) were followed up for 7 to 14 days, finally, a total of 49 subjects were followed up and further divided into cure (n = 35) and none-cure (n = 14) subgroups according to urine culture results...
February 19, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Christopher Foley, Arthur Shaw, Christopher Hulme
Direct oxidative deamination of glyoxal-derived Ugi-azide and Ugi three-component reaction products readily affords vicinal tricarbonyls (α,β-diketoamides) and α,β-diketotetrazoles with two diversity elements. This significant extension of our previously described multicomponent reaction-oxidative deamination methodology is proposed to proceed through a mechanistically distinct SeO2 -mediated C-N oxidation derived from an active enol of α-amino-β-ketone systems, effectively an aza-Riley oxidation. This methodology accesses diverse VTC systems from prototypical amines, glyoxaldehydes, and isocyanide building blocks in a mere two steps...
February 21, 2018: Organic Letters
Bin Huang, Yuting He, Xingyan Ma, Renxin Cai, Jianming Zeng, Yang Lu, Weizheng Zhang, Kai Lan, Sunmei E, Yi-Wei Tang, Barry N Kreiswirth, Cha Chen, Liang Chen
Next generation sequencing of six mcr-1- harboring Escherichia coli and Klebsiella pneumoniae isolates collected from a tertiary care hospital in China revealed significant sequence variations in the regions flanking the mcr-1 gene. While sequence variations significantly affect the expression and promoter activity of mcr-1 , the mcr-1 gene expressions do not correlate with the in vitro colistin resistance levels, which warrants further in-depth investigations.
February 20, 2018: Antimicrobial Agents and Chemotherapy
Jilei Zhang, Li Chen, Jiawei Wang, Patrick Butaye, Ke Huang, Haixiang Qiu, Xiaomei Zhang, Weijuan Gong, Chengming Wang
OBJECTIVE: Colistin resistance has emerged worldwide and has been threatening the efficacy of one of the last-resort antimicrobials used for treatment of multidrug resistant Gram-negative bacteria. While five colistin resistance genes (mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5) have been described, few data are available on the prevalence of mcr-genes other than mcr-1 in human samples. RESULTS: In this study, the presence of five currently described colistin resistance genes (mcr 1-5) in vaginal swabs of women undergoing infertility evaluation was reported...
February 20, 2018: BMC Research Notes
Yongchang Xu, Jingxia Lin, Tao Cui, Swaminath Srinivas, Youjun Feng
Polymyxins such as colistin are antibiotics used as a final line of defense in the management of infections with multidrug-resistant Gram-negative bacteria. Although natural resistance to polymyxins is rare, the discovery of a mobilized colistin resistance gene ( mcr-1 ) in gut bacteria has raised significant concern. As an intramembrane enzyme, MCR-1 catalyzes the transfer of phosphoethanolamine (PEA) to the 1 (or 4')-phosphate group of the lipid A moiety of lipopolysaccharide (LPS), thereby conferring colistin resistance...
February 9, 2018: Journal of Biological Chemistry
Jiyun Li, Anette Hulth, Lennart E Nilsson, Stefan Börjesson, Baoli Chen, Zhenwang Bi, Yang Wang, Stefan Schwarz, Congming Wu
No abstract text is available yet for this article.
February 16, 2018: Journal of Antimicrobial Chemotherapy
Plamen Katsarov, Georgi Gergov, Aylin Alin, Bissera Pilicheva, Yahya Al-Degs, Vasil Simeonov, Margarita Kassarova
The prediction power of partial least squares (PLS) and multivariate curve resolution-alternating least squares (MCR-ALS) methods have been studied for simultaneous quantitative analysis of the binary drug combination - doxylamine succinate and pyridoxine hydrochloride. Analysis of first-order UV overlapped spectra was performed using different PLS models - classical PLS1 and PLS2 as well as partial robust M-regression (PRM). These linear models were compared to MCR-ALS with equality and correlation constraints (MCR-ALS-CC)...
March 1, 2018: Acta Pharmaceutica
F Del Bianco, M Morotti, M F Pedna, P Farabegoli, V Sambri
OBJECTIVES: To start a surveillance program to investigate the possible diffusion of mobilized colistin resistance genes in Enterobacteriaceae strains isolated in the Unit of Microbiology of the Great Romagna Hub Laboratory. METHODS: All the colistin resistant Enterobacteriaceae, isolated from August 1st 2016 to July 31st 2017, were prospectively evaluated for mcr-1 a mcr-2. Backdated survey of mcr-3, mcr-4 and mcr-5 was performed on the same group of isolates. Species identification was achieved by Vitek MS and the antibiotic susceptibility testing was performed both with Vitek-2 and Sensititre systems...
February 12, 2018: International Journal of Infectious Diseases: IJID
Qidong Wang, Chuangen Guo, Lan Zhang, Rui Zhang, Zhaoming Wang, Ying Xu, Wenbo Xiao
Objective To investigate changes in renal oxygenation levels by blood-oxygenation-level dependent (BOLD)-magnetic resonance imaging (MRI), and to evaluate BOLD-MRI for detecting early diabetic renal injury. Methods Seventy-five rats, with unilateral nephrectomy, were randomly divided into streptozotocin-induced diabetes mellitus (DM, n = 65) and normal control (NC, n = 10) groups. BOLD-MRI scans were performed at baseline (both groups) and at 3, 7, 14, 21, 28, 35, 42, 49, 56, 63 and 70 days (DM only). Renal cortical (C) and medullary (M) R2* signals were measured and R2* medulla/cortex ratio (MCR) was calculated...
January 1, 2018: Journal of International Medical Research
Jens A Hammerl, Maria Borowiak, Silvia Schmoger, Dina Shamoun, Mirjam Grobbel, Burkhard Malorny, Bernd-Alois Tenhagen, Annemarie Käsbohrer
No abstract text is available yet for this article.
February 12, 2018: Journal of Antimicrobial Chemotherapy
Wai-Sing Chan, Chun-Hang Au, Dona N Ho, Tsun-Leung Chan, Edmond Shiu-Kwan Ma, Bone Siu-Fai Tang
BACKGROUND: Human fecal carriage of Enterobacteriaceae possessing mobilized colistin resistance genes (mcr-1 and mcr-2) remains obscure in Hong Kong. As part of routine surveillance on emerging antibiotic resistance, we conducted a prospective study on this topic in a regional hospital in Hong Kong. METHODS: From October 31 to November 25, 2016, all fecal specimens submitted for routine analysis were included in this surveillance study. These comprised 672 consecutive routine fecal specimens collected from 616 individuals...
February 13, 2018: BMC Infectious Diseases
Camila Y Bastidas, Carlos von Plessing, José Troncoso, Rosario Del P Castillo
Fourier Transform infrared imaging and multivariate analysis were used to identify, at the microscopic level, the presence of florfenicol (FF), a heavily-used antibiotic in the salmon industry, supplied to fishes in feed pellets for the treatment of salmonid rickettsial septicemia (SRS). The FF distribution was evaluated using Principal Component Analysis (PCA) and Augmented Multivariate Curve Resolution with Alternating Least Squares (augmented MCR-ALS) on the spectra obtained from images with pixel sizes of 6...
February 7, 2018: Journal of Pharmaceutical and Biomedical Analysis
Rocío Ríos-Reina, M Lourdes Morales, Diego L García-González, José M Amigo, Raquel M Callejón
High-quality wine vinegars have been registered in Spain under protected designation of origin (PDO): "Vinagre de Jerez", "Vinagre de Condado de Huelva" and "Vinagre de Montilla-Moriles". The raw material, production and aging processes determine their quality and their aromatic composition. Vinegar volatile profile is usually analyzed by gas chromatography-mass spectrometry (GC-MS), being necessary a previous extraction step. Thus, three different sampling methods (Headspace solid phase microextraction "HS-SPME", Headspace stir bar sorptive extraction "HSSE" and Dynamic headspace extraction "DHS") were studied for the analysis of the volatile composition of Spanish PDO wine vinegars...
March 2018: Food Research International
Silvia De Luca, Eleonora Ciotoli, Alessandra Biancolillo, Remo Bucci, Andrea D Magrì, Federico Marini
A chromatographic procedure (HPLC-DAD) using a relatively rapid gradient has been combined with a chemometric curve deconvolution method, multivariate curve resolution-alternating least squares (MCR-ALS), in order to quantify caffeine and chlorogenic acid in green coffee beans. Despite that the HPLC analysis (at these specific operating conditions) presents some coeluting peaks, MCR-ALS allowed their resolution and, consequently, the creation of a calibration curve to be used for the quantification of the analytes of interest; this procedure led to a high accuracy in the quantification of caffeine and chlorogenic acid present in the samples...
February 11, 2018: Environmental Science and Pollution Research International
Laura Pont, Victoria Sanz-Nebot, Marta Vilaseca, Joaquim Jaumot, Roma Tauler, Fernando Benavente
In this study, we describe a chemometric data analysis approach to assist in the interpretation of the complex datasets from the analysis of high-molecular mass oligomeric proteins by ion mobility mass spectrometry (IM-MS). The homotetrameric protein transthyretin (TTR) is involved in familial amyloidotic polyneuropathy type I (FAP-I). FAP-I is associated with a specific TTR mutant variant (TTR(Met30)) that can be easily detected analyzing the monomeric forms of the mutant protein. However, the mechanism of protein misfolding and aggregation onset, which could be triggered by structural changes in the native tetrameric protein, remains under investigation...
May 1, 2018: Talanta
Yi-Ming Wang, Ling-Cong Kong, Jie Liu, Hong-Xia Ma
Background: Bacterial infections have become more challenging to treat due to the emergence of multidrug-resistant pathogenic bacteria. Combined antibiotics prove to be a relatively effective method to control such resistant strains. This study aim to investigate synergistic activity of eugenol combined with colistin against a collection of clinical isolated Escherichia coli (E.coli) strains, and to evaluate potential interaction. Methods: Antimicrobial susceptibility, minimum inhibitory concentration (MIC) and fractional inhibitory concentration index (FICI) of the bacteria were determined by disk diffusion assay, broth microdilution method and checkerboard assay, respectively...
2018: Antimicrobial Resistance and Infection Control
Elaa Maamar, Carla Andrea Alonso, Zaineb Hamzaoui, Nouha Dakhli, Mohamed Salah Abbassi, Sana Ferjani, Mabrouka Saidani, Ilhem Boutiba-Ben Boubaker, Carmen Torres
Our study aimed to investigate colistin resistance and the mechanisms involved in a collection of 35 extended-spectrum beta-lactamase (ESBL) and 13 CMY-2-producing E. coli strains which were previously recovered from chicken gut microbiota in Tunisia, as well as to determine the genetic location of mcr genes. Forty-eight ESBL and CMY-2-producing E. coli strains were obtained from 137 fecal samples of healthy chickens during 2013. These strains were tested for colistin resistance by the broth microdilution method, and screened for mcr-1 and mcr-2 genes by PCR...
January 29, 2018: International Journal of Food Microbiology
Liliane Okdah, Stéphanie Le Page, Abiola Olumuyiwa Olaitan, Grégory Dubourg, Linda Hadjadj, Jean-Marc Rolain
BACKGROUND: The recent emergence of colistin resistance, particularly mcr-1 plasmid-mediated colistin resistance in Gram negative bacteria, has led to renewed interest in antibiotic combinations to overcome clinical therapeutic impasses. OBJECTIVE: We aim to evaluate the potential of the synergistic and bactericidal activity of colistin in combination with sulphonamide compounds including sulfadiazine, sulfamethoxazole, and trimethoprim-sulfamethoxazole, against clinical colistin-resistant bacterial strains, including strains with the plasmidic mcr-1encoding gene...
February 5, 2018: International Journal of Antimicrobial Agents
Xiangen Wu, Wanjun Lu, Louis M Streacker, Henry S Ashbaugh, Dor Ben-Amotz
Experimental Raman multivariate curve resolution (Raman-MCR) and molecular dynamics (MD) simulations are performed to demonstrate that the vibrational frequency and tetrahedrality of water molecules in the hydration-shells of short-chain alcohols differ from pure water and undergo a crossover above 100○C (at 30 MPa) to a structure that is less tetrahedral than pure water. Our results demonstrate that the associated crossover length scale decreases with increasing temperature, suggesting that there is a fundamental connection between the spectroscopically observed crossover and that predicted to take place around idealized purely-repulsive solutes dissolved in water, although the water structure changes in the hydration-shells of alcohols are far smaller than those associated with an idealized "dewetting" transition...
February 8, 2018: Journal of Physical Chemistry Letters
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