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https://www.readbyqxmd.com/read/28424528/elucidation-of-quantitative-structural-diversity-of-remarkable-rearrangement-regions-shufflons-in-inci2-plasmids
#1
Tsuyoshi Sekizuka, Michiko Kawanishi, Mamoru Ohnishi, Ayaka Shima, Kengo Kato, Akifumi Yamashita, Mari Matsui, Satowa Suzuki, Makoto Kuroda
A multiple DNA inversion system, the shufflon, exists in incompatibility (Inc) I1 and I2 plasmids. The shufflon generates variants of the PilV protein, a minor component of the thin pilus. The shufflon is one of the most difficult regions for de novo genome assembly because of its structural diversity even in an isolated bacterial clone. We determined complete genome sequences, including those of IncI2 plasmids carrying mcr-1, of three Escherichia coli strains using single-molecule, real-time (SMRT) sequencing and Illumina sequencing...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28421056/high-prevalence-of-colistin-resistance-and-mcr-1-gene-in-escherichia-coli-isolated-from-food-animals-in-china
#2
Xianhui Huang, Linfeng Yu, Xiaojie Chen, Chanping Zhi, Xu Yao, Yiyun Liu, Shengjun Wu, Zewen Guo, Linxian Yi, Zhenling Zeng, Jian-Hua Liu
The objective of this study was to determine the minimal inhibitory concentration of colistin for Escherichia coli from food animals and the possible underlying colistin resistance mechanisms. During 2007-2014, 4,438 E. coli isolates of food animal origins were collected. The susceptibility of colistin was tested by the agar dilution method. Mutations in pmrA, pmrB, and mgrB and the presence of mcr-1 gene were determined by PCR and DNA sequencing. Complementation experiments were carried out to evaluate the contribution of the mutations to colistin resistance...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28420384/molecular-characteristic-of-mcr-1-producing-escherichia-coli-in-a-chinese-university-hospital
#3
Qing-Wen He, Xiao-Hong Xu, Fang-Jun Lan, Zhi-Chang Zhao, Zhi-Yun Wu, Ying-Ping Cao, Bin Li
BACKGROUND: Colistin has been considered as a last-line treatment option in severe infections caused by multidrug-resistant (MDR) gram-negative pathogens. However, the emergence of the mobile colistin resistance gene (mcr-1) has challenged this viewpoint. The aim of this study is to explore the prevalence of mcr-1 in Escherichia coli (E. coli) in a Chinese teaching hospital, and investigate their molecular characteristics. METHODS: A total of 700 E. coli isolates were used to screen mcr-1 by PCR and sequencing in a Chinese university hospital from August 2014 to August 2015...
April 19, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28418313/mcr-1-colistin-resistance-in-esbl-producing-klebsiella-pneumoniae-france
#4
Yvan Caspar, Mylène Maillet, Patricia Pavese, Gilles Francony, Jean-Paul Brion, Marie-Reine Mallaret, Richard Bonnet, Frédéric Robin, Racha Beyrouthy, Max Maurin
We report intestinal carriage of an extended-spectrum β-lactamase-producing Klebsiella pneumoniae strain with high-level resistance to colistin (MIC 24 mg/L) in a patient in France who had been hospitalized for fungal meningitis. The strain had the mcr-1 plasmid gene and an inactivated mgrB gene, which are associated with colistin resistance.
May 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28416556/colistin-resistant-mcr-1-positive-escherichia-coli-in-public-beaches-an-infectious-threat-emerging-in-recreational-waters
#5
Miriam R Fernandes, Fábio P Sellera, Fernanda Esposito, Caetano P Sabino, Louise Cerdeira, Nilton Lincopan
The emergence and rapid spread of colistin-resistant Escherichia coli carrying the mcr-1 gene has generated an urgent need to strengthen surveillance. We have performed a meticulous investigation of strains of this sort, resulting in the first identification of international clones of E. coli carrying IncX4 plasmid-mediated mcr-1 and blaCTX-M genes in recreational waters of public urban beaches in cities with high tourist turnover, highlighting a new environmental reservoir.
April 17, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28416554/in-vitro-study-of-isapl1-mediated-mobilization-of-the-colistin-resistance-gene-mcr-1
#6
Laurent Poirel, Nicolas Kieffer, Patrice Nordmann
The plasmid-mediated mcr-1 gene encodes a phosphoethanolamine transferase conferring resistance to polymyxins. The mcr-1 gene is associated with insertion sequence ISApl1 (IS30 family). In-vitro mobilization assays demonstrated the functionality of the composite transposon structure ISApl1-mcr-1-ISApl1 Transposition generated a 2-bp duplication and occurred in AT-rich DNA regions. This is the first report demonstrating the mobility of the mcr-1 gene by transposition.
April 17, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28416545/dissemination-of-mcr-1-carrying-plasmids-among-colistin-resistant-salmonella-strains-from-humans-and-food-producing-animals-taiwan
#7
Chien-Shun Chiou, Ying-Tsong Chen, You-Wun Wang, Yen-Yi Liu, Hung-Chih Kuo, Yeuh-Hua Tu, Ann-Chi Lin, Ying-Shu Liao, Yu-Ping Hong
We detected the colistin-resistance gene mcr-1 in four Salmonella serovars isolated from humans and animals with diarrhea. The resistance gene was carried on different plasmids. One mcr-1-carrying conjugative plasmid, a variant of pHNSHP45, was disseminated among Salmonella isolates recovered from humans, pigs, and chickens.
April 17, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28412861/the-association-between-progestins-nuclear-receptors-expression-and-inflammation-in-endometrial-stromal-cells-from-women-with-endometriosis
#8
Giovanni Grandi, Michael D Mueller, Nick A Bersinger, Fabio Facchinetti, Brett D McKinnon
Endometriosis is an inflammatory disease and nuclear receptors play a crucial role in mediating the inflammatory response. In endometrial stromal cells (ESC), nuclear receptors expression can be influenced by the local environment. Progestins are first-line, on-label treatments of endometriosis that may have direct effects on endometriotic lesions through these nuclear receptors. Therefore, we investigated whether there was an association between nuclear receptors expression and the influence of progestins on inflammatory cytokines production in a preliminary, in vitro study with primary cultures...
April 17, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28411495/arbuscular-mycorrhiza-formation-and-its-function-under-elevated-atmospheric-o3-a-meta-analysis
#9
REVIEW
Shuguang Wang, Robert M Augé, Heather D Toler
We quantitatively evaluated the effects of elevated O3 on arbuscular mycorrhiza (AM) formation and on AM role in promoting plant growth in regard to several moderating variables (O3 levels, O3 exposure duration, plant types, AM fungi family, and additional stress) by means of meta-analysis of published data. The analysis consisted of 117 trials representing 20 peer-reviewed articles and 16 unpublished trials. Relative to non-mycorrhizal controls, AM inoculation did not significantly alter plant growth (shoot biomass, root biomass, total biomass and plant height) when O3 concentration was less than 80 ppb, but at concentrations above 80 ppb symbiosis was associated with increases of 68% in shoot biomass and 131% in root biomass...
April 12, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28409929/oxidative-deaminations-and-deisatinylations-of-ugi-azide-and-ugi-3cr-products-a-two-step-mcr-oxidation-protocol-toward-functionalized-%C3%AE-ketoamides-and-%C3%AE-ketotetrazoles
#10
Christopher Foley, Arthur Shaw, Christopher Hulme
A new postcondensation multicomponent reaction (MCR) methodology, comprising oxidative deaminations enabling access to multiple privileged carbonyl-containing scaffolds in two steps, is described. These protocols allow facile access to functionalized α-ketoamide and α-ketotetrazole small-molecule peptidomimetic-like building blocks from prototypical synthons with two points of diversity. Incorporation of chalcone and alkynyl moieties with further ring-forming reactions enables access to additional novel heterocyclic ring systems, including a unique and potentially highly pharmacologically relevant scaffold, a 1,2-selenazol-3(2H)-one...
April 14, 2017: Organic Letters
https://www.readbyqxmd.com/read/28400211/a-sybr-%C3%A2-green-based-real-time-pcr-method-for-improved-detection-of-mcr-1-mediated-colistin-resistance-in-human-stool-samples
#11
Valentina Donà, Odette J Bernasconi, Sara Kasraian, Regula Tinguely, Andrea Endimiani
OBJECTIVES: The aim of this study was to design a rapid and sensitive real-time PCR (rt-PCR) method for colistin resistance mcr-1 gene detection in human faecal samples. METHODS: Stools (n=88) from 36 volunteers were analysed. To isolate mcr-1-producing Enterobacteriaceae, samples were enriched overnight in Luria-Bertani (LB) broth containing 2mg/L colistin and were then plated on selective agar plates with 4mg/L colistin. A SYBR(®) Green-based rt-PCR targeting mcr-1 was then designed...
April 8, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28396167/comparative-study-of-ann-and-rsm-for-simultaneous-optimization-of-multiple-targets-in-fenton-treatment-of-landfill-leachate
#12
Mohammad Reza Sabour, Allahyar Amiri
In this study, two modeling methods, namely response surface methodology (RSM) and artificial neural networks (ANN), were applied to investigate the Fenton process performance in landfill leachate treatment. For this purpose, three targets were used to cover different aspects of post-treatment products such as supernatant and sludge: mass content ratio (MCR) and mass removal efficiency (MRE). It was observed that coagulation was dominant mechanism in all responses. The proposed models were evaluated based on correlation coefficient (R(2)), root mean square error (RMSE) and average error (AE) and both models seemed satisfactory...
April 7, 2017: Waste Management
https://www.readbyqxmd.com/read/28386512/study-of-chemical-intermediates-by-means-of-atr-ir-spectroscopy-and-hybrid-hard-and-soft-modelling-multivariate-curve-resolution-alternating-least-squares
#13
Junxiu Ma, Juan Qi, Xinyu Gao, Chunhua Yan, Tianlong Zhang, Hongsheng Tang, Hua Li
3,5-Diamino-1,2,4-triazole (DAT) became a significant energetic materials intermediate, and the study of its reaction mechanism has fundamental significance in chemistry. The aim of this study is to investigate the ability of online attenuated total reflection infrared (ATR-IR) spectroscopy combined with the novel approach of hybrid hard- and soft-modelling multivariate curve resolution-alternating least squares (HS-MCR) analysis to monitor and detect changes in structural properties of compound during 3,5-diamino-1,2,4-triazole (DAT) synthesis processes...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28383517/antibiotic-resistance-in-an-indian-rural-community-a-one-health-observational-study-on-commensal-coliform-from-humans-animals-and-water
#14
Manju Raj Purohit, Salesh Chandran, Harshada Shah, Vishal Diwan, Ashok J Tamhankar, Cecilia Stålsby Lundborg
Antibiotic-resistant bacteria are an escalating grim menace to global public health. Our aim is to phenotype and genotype antibiotic-resistant commensal Escherichia coli (E. coli) from humans, animals, and water from the same community with a 'one-health' approach. The samples were collected from a village belonging to demographic surveillance site of Ruxmaniben Deepchand (R.D.) Gardi Medical College Ujjain, Central India. Commensal coliforms from stool samples from children aged 1-3 years and their environment (animals, drinking water from children's households, common source- and waste-water) were studied for antibiotic susceptibility and plasmid-encoded resistance genes...
April 6, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28378841/%C3%AE-msh-and-melanocortin-receptors-at-early-ontogeny-in-european-sea-bass-dicentrarchus-labrax-l
#15
A Tsalafouta, M Gorissen, T N M Pelgrim, N Papandroulakis, G Flik, M Pavlidis
Temporal patterns of whole-body α-MSH concentrations and of transcripts of melanocortin receptors during early development as well as the endocrine response (α-MSH, cortisol, MCR mRNAs) to stress at the end of the larval period were characterized in Dicentrarchus labrax. Immunohistochemistry showed α-MSH positive cells in the pituitary pars intermedia in all stages examined. As development proceeds, α-MSH content gradually increases; mRNA levels of mc2r and mc4r remain low until first feeding where peak values are observed...
April 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28374600/diffuse-reflectance-spectroscopy-of-hidden-objects-part-ii-recovery-of-a-target-spectrum
#16
Alexey L Pomerantsev, Oxana Ye Rodionova, Alexej N Skvortsov
In this study, we consider the reconstruction of a diffuse reflectance near-infrared spectrum of an object (target spectrum) in case the object is covered by an interfering absorbing and scattering layer. Recovery is performed using a new empirical method, which was developed in our previous study. We focus on a system, which consists of several layers of polyethylene (PE) film and underlayer objects with different spectral features. The spectral contribution of the interfering layer is modeled by a three-component two-parameter multivariate curve resolution (MCR) model, which was built and calibrated using spectrally flat objects...
January 1, 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28373993/efficacy-of-administration-of-an-angiotensin-converting-enzyme-inhibitor-for-two-years-on-autonomic-and-peripheral-neuropathy-in-patients-with-diabetes-mellitus
#17
Triantafyllos Didangelos, Konstantinos Tziomalos, Charalambos Margaritidis, Zisis Kontoninas, Ioannis Stergiou, Stefanos Tsotoulidis, Eleni Karlafti, Alexandros Mourouglakis, Apostolos I Hatzitolios
Aim. To evaluate the effect of quinapril on diabetic cardiovascular autonomic neuropathy (CAN) and peripheral neuropathy (DPN). Patients and Methods. Sixty-three consecutive patients with diabetes mellitus [43% males, 27 with type 1 DM, mean age 52 years (range 22-65)], definite DCAN [abnormal results in 2 cardiovascular autonomic reflex tests (CARTs)], and DPN were randomized to quinapril 20 mg/day (group A, n = 31) or placebo (group B, n = 32) for 2 years. Patients with hypertension or coronary heart disease were excluded...
2017: Journal of Diabetes Research
https://www.readbyqxmd.com/read/28373195/structural-modification-of-lipopolysaccharide-conferred-by-mcr-1-in-gram-negative-eskape-pathogens
#18
Yi-Yun Liu, Courtney E Chandler, Lisa M Leung, Christi L McElheny, Roberta T Mettus, Robert M Q Shanks, Jian-Hua Liu, David R Goodlett, Robert K Ernst, Yohei Doi
mcr-1 was initially reported as the first plasmid-mediated colistin resistance gene in clinical isolates of Escherichia coli and Klebsiella pneumoniae in China and has subsequently been identified worldwide in various species of the family Enterobacteriaceae. mcr-1 encodes a phosphoethanolamine transferase and its expression has been shown to generate phosphoethanolamine-modified bis-phosphorylated hexa-acylated lipid A in E. coli Here, we investigated the effect of mcr-1 on colistin susceptibility and lipopolysaccharide structures in laboratory and clinical strains of the Gram-negative ESKAPE pathogens, which are often treated by colistin clinically...
April 3, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28373188/molecular-characterization-of-escherichia-coli-isolates-carrying-mcr-1-fosa3-and-esbl-genes-from-food-samples-in-china
#19
Xiaobo Liu, Ruichao Li, Zhiwei Zheng, Kaichao Chen, Miaomiao Xie, Edward Wai-Chi Chan, Shu Geng, Sheng Chen
The study surveyed the prevalence of mcr-1 in ESBL-producing E. coli of food origin in China and identified strains that carried mcr-1, fosA3 and ESBL genes, which were encoded in various plasmids. The mcr-1 and ESBL genes could be co-transferred by one or different types of plasmids. The presence of these multiple-drug-resistant E. coli strains in our food products might pose huge threat to public health.
April 3, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28370547/real-time-monitoring-of-enzyme-catalysed-reactions-using-deep-uv-resonance-raman-spectroscopy
#20
Chloe Westley, Heidi Fisk, Yun Xu, Katherine Hollywood, Andrew Carnell, Jason Micklefield, Nicholas Turner, Royston Goodacre
For enzyme-catalysed biotransformations, continuous in situ detection methods minimise the need for sample manipulation, ultimately leading to more accurate real-time kinetic determinations of substrate(s) and product(s). We have established for the first time an on-line, real-time quantitative approach to monitor simultaneously multiple biotransformations based on UV resonance Raman (UVRR) spectroscopy. In order to exemplify the generality and versatility of this approach, multiple substrates and enzyme systems were used involving nitrile hydratase and xanthine oxidase, both of which are of industrial and biological significance, and incorporating multi-step enzymatic conversions...
March 29, 2017: Chemistry: a European Journal
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