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https://www.readbyqxmd.com/read/28541467/global-epidemiology-of-ctx-m-%C3%AE-lactamases-temporal-and-geographical-shifts-in-genotype
#1
Edward R Bevan, Annie M Jones, Peter M Hawkey
Globally, rates of ESBL-producing Enterobacteriaceae are rising. We undertook a literature review, and present the temporal trends in blaCTX-M epidemiology, showing that blaCTX-M-15 and blaCTX-M-14 have displaced other genotypes in many parts of the world. Explanations for these changes can be attributed to: (i) horizontal gene transfer (HGT) of plasmids; (ii) successful Escherichia coli clones; (iii) ESBLs in food animals; (iv) the natural environment; and (v) human migration and access to basic sanitation...
May 25, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28537547/increasing-proportion-of-carbapenemase-producing-enterobacteriaceae-and-emergence-of-a-mcr-1-producer-through-a-multicentric-study-among-hospital-based-and-private-laboratories-in-belgium-from-september-to-november-2015
#2
Te Din Huang, Pierre Bogaerts, Catherine Berhin, Martin Hoebeke, Caroline Bauraing, Youri Glupczynski
Carbapenemase-producing Enterobacteriaceae (CPE) strains have been increasingly reported in Belgium. We aimed to determine the proportion of CPE among Enterobacteriaceae isolated from hospitalised patients and community outpatients in Belgium in 2015. For the hospitalised patients, the results were compared to a previous similar survey performed in the same hospitals in 2012. Twenty-four hospital-based and 10 private laboratories collected prospectively 200 non-duplicated Enterobacteriaceae isolates from clinical specimens...
May 11, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28535607/generation-of-the-new-discovered-resistant-gene-mcr-1-knockout-in-escherichia-coli-using-crispr-cas9-system
#3
Lichang Sun, Tao He, Lili Zhang, Maoda Pang, Qiaoyan Zhang, Yan Zhou, Hongduo Bao, Ran Wang
The mcr-1 gene was the new discovered "superbug" gene which makes the bacteria highly resistant to the last-resort class of antibiotics in China in 2016. The mcr-1 gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy that CRISPR/Cas9 mediated approach can efficiently induce mcr-1 gene knockout in Escherichia coli. Our findings suggested that using CRISPPR/Cas9 system to knockout the resistant gene mcr-1 might be a potential anti-resistant strategy...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28526080/post-weaning-diarrhea-in-pigs-risk-factors-and-non-colistin-based-control-strategies
#4
REVIEW
Mohamed Rhouma, John Morris Fairbrother, Francis Beaudry, Ann Letellier
Post-weaning diarrhea (PWD) is one of the most serious threats for the swine industry worldwide. It is commonly associated with the proliferation of enterotoxigenic Escherichia coli in the pig intestine. Colistin, a cationic antibiotic, is widely used in swine for the oral treatment of intestinal infections caused by E. coli, and particularly of PWD. However, despite the effectiveness of this antibiotic in the treatment of PWD, several studies have reported high rates of colistin resistant E. coli in swine...
May 19, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28522705/draft-genome-sequences-of-colistin-resistant-mcr-1-producing-escherichia-coli-st1850-and-st74-strains-isolated-from-commercial-chicken-meat
#5
Daniel F Monte, Miriam R Fernandes, Louise Cerdeira, Tiago A de Souza, Andressa Mem, Bernadette D G M Franco, Mariza Landgraf, Nilton Lincopan
We present here the draft genome sequences of two colistin-resistant mcr-1-carrying Escherichia coli strains belonging to sequence type 74 (ST74) and ST1850, isolated from commercial chicken meat in Brazil. Assembly of this draft genome resulted in 5,022,083 and 4,950,681 bp, respectively, revealing the presence of the IncX4 plasmid-mediated mcr-1 gene responsible for resistance to colistin.
May 18, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28507112/mcr-1-and-oxa-48-in-vivo-acquisition-in-kpc-producing-escherichia-coli-after-colistin-treatment
#6
Racha Beyrouthy, Frederic Robin, Aude Lessene, Igor Lacombat, Laurent Dortet, Thierry Naas, Valérie Ponties, Richard Bonnet
The spread of mcr-1-encoding plasmids into carbapenem-resistant Enterobacteriaceae raises concerns about the emergence of untreatable bacteria. We report the acquisition of mcr-1 in a carbapenem- resistant E. coli after a 3-week course of colistin in a patient repatriated to France from Portugal. Whole-genome sequencing revealed that the KPC-producing E. coli strain acquired two plasmids, an IncL OXA-48-encoding plasmid and an IncX4 mcr-1-encoding plasmid. This is the first report of mcr-1 in carbapenemase-encoding bacteria in France...
May 15, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28505353/co-occurrence-of-mcr-1-and-blakpc-2-in-a-clinical-isolate-of-escherichia-coli-in-brazil
#7
Tanise Vendruscolo Dalmolin, Luiza Castro, Fabiana Q Mayer, Alexandre P Zavascki, Andreza Francisco Martins, Daiana de Lima-Morales, Afonso Luís Barth
No abstract text is available yet for this article.
May 15, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28505268/pharmacodynamics-of-dose-escalated-front-loading-polymyxin-b-regimens-against-polymyxin-resistant-mcr-1-harbouring-escherichia-coli
#8
Nicholas M Smith, Zackery P Bulman, Arthur O Sieron, Jürgen B Bulitta, Patricia N Holden, Roger L Nation, Jian Li, Gerard D Wright, Brian T Tsuji
Objectives: Gram-negative bacteria harbouring the mcr-1 plasmid are resistant to the 'last-line' polymyxins and have been reported worldwide. Our objective was to define the impact of increasing the initial polymyxin B dose intensity against an mcr-1 -harbouring strain to delineate the impact of plasmid-mediated polymyxin resistance on the dynamics of bacterial killing and resistance. Methods: A hollow fibre infection model (HFIM) was used to simulate polymyxin B regimens against an mcr-1 -harbouring Escherichia coli (MIC 8 mg/L) over 10 days...
May 15, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28501714/determination-of-polymer-crystallinity-by-the-multivariable-curve-resolution-method-in-13-c-solid-nmr-spectrum
#9
Xiaoyue Zhou, Kaipin Xu, Pei Ni, Qun Chen, Shanmin Zhang
Most of the polymers are composed of a crystal part, an amorphous part, and a transitional interfacial part. These components present disparate physical and chemical characteristics. However, it always suffers from peak overlapping in solid NMR spectrum in order to acquire polymer's crystallinity. The polyethylene oxide (PEO) sample was tested using the Torchia pulse sequence combined with the Multivariate Curve Resolution (MCR) method. A two dimensional CP/MAS spectrum containing spin-lattice relaxation time (T1) information was acquired...
April 27, 2017: Solid State Nuclear Magnetic Resonance
https://www.readbyqxmd.com/read/28487432/deciphering-mcr-2-colistin-resistance
#10
Jian Sun, Yongchang Xu, Rongsui Gao, Jingxia Lin, Wenhui Wei, Swaminath Srinivas, Defeng Li, Run-Shi Yang, Xing-Ping Li, Xiao-Ping Liao, Ya-Hong Liu, Youjun Feng
Antibiotic resistance is a prevalent problem in public health worldwide. In general, the carbapenem β-lactam antibiotics are considered a final resort against lethal infections by multidrug-resistant bacteria. Colistin is a cationic polypeptide antibiotic and acts as the last line of defense for treatment of carbapenem-resistant bacteria. Very recently, a new plasmid-borne colistin resistance gene, mcr-2, was revealed soon after the discovery of the paradigm gene mcr-1, which has disseminated globally. However, the molecular mechanisms for MCR-2 colistin resistance are poorly understood...
May 9, 2017: MBio
https://www.readbyqxmd.com/read/28483954/genetic-and-functional-characterization-of-blactx-m-199-a-novel-tazobactam-and-sulbactam-resistance-encoding-gene-located-in-a-conjugative-mcr-1-bearing-inci2-plasmid
#11
Jiachang Cai, Qipeng Cheng, Yingbo Shen, Danxia Gu, Ying Fang, Edward Wai-Chi Chan, Sheng Chen
The study reported the genetic and functional characterization of a novel CTX-M-199 β-lactamase, which was encoded by a blaCTX-M-64 variant gene found in a conjugative mcr-1-bearing IncI2 plasmid and exhibited resistance to β-lactamase inhibitors, tazobactam and sulbactam.
May 8, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28481978/application-of-the-cghra-framework-to-the-genomic-characterization-of-diffuse-large-b-cell-lymphoma
#12
Sylvain Mareschal, Philippe Ruminy, Marion Alcantara, Céline Villenet, Martin Figeac, Sydney Dubois, Philippe Bertrand, Abdelilah Bouzelfen, Pierre-Julien Viailly, Dominique Penther, Hervé Tilly, Christian Bastard, Fabrice Jardin
Motivation: Although sequencing-based technologies are becoming the new reference in genome analysis, comparative genomic hybridization arrays (aCGH) still constitute a simple and reliable approach for copy number analysis. The most powerful algorithms to analyse such data have been freely provided by the scientific community for many years, but combining them is a complex scripting task. Results: The cghRA framework combines a user-friendly graphical interface and a powerful objectoriented command-line interface to handle a full aCGH analysis, as is illustrated in an original series of 107 Diffuse Large B-Cell Lymphomas...
May 8, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28480991/unraveling-the-multistimuli-responses-of-a-complex-dynamic-system-of-pseudopeptidic-macrocycles
#13
Angel M Valdivielso, Francesc Puig-Castellví, Joan Atcher, Jordi Solà, Romà Tauler, Ignacio Alfonso
Dynamic combinatorial libraries (DCLs) are excellent benchmark models to study the stimuli-responsiveness of chemical networks. However, increasingly complex systems are difficult to analyze with simple data analysis methods, because many variables and connections must be considered for their full understanding. Here we propose the use of multivariate data analysis methods to bisect the evolution of a complex synthetic dynamic library of pseudopeptidic macrocycles, containing side chains with charges of different sign...
May 8, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28480907/metabolomic-analysis-of-the-effects-of-cadmium-and-copper-treatment-in-oryza-sativa-l-using-untargeted-liquid-chromatography-coupled-to-high-resolution-mass-spectrometry-and-all-ion-fragmentation
#14
Meritxell Navarro-Reig, Joaquim Jaumot, Benjamín Piña, Encarnación Moyano, Maria Teresa Galceran, Romà Tauler
While the knowledge of plant metabolomes has increased in the last few years, their response to the presence of toxicants is still poorly understood. Here, we analyse the metabolomic changes in Japanese rice (Oryza sativa var. Japonica) upon exposure to heavy metals (Cd(ii) and Cu(ii)) in concentrations from 10 to 1000 μM. After harvesting, rice metabolites were extracted from aerial parts of the plants and analysed by HPLC (HILIC TSK gel amide-80 column) coupled to a mass spectrometer quadrupole-Orbitrap (Q-Exactive)...
May 8, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28479289/nationwide-surveillance-of-clinical-carbapenem-resistant-enterobacteriaceae-cre-strains-in-china
#15
Rong Zhang, Lizhang Liu, Hongwei Zhou, Edward Waichi Chan, Jiaping Li, Ying Fang, Yi Li, Kang Liao, Sheng Chen
The increasing incidence of carbapenem-resistant Enterobacteriaceae (CRE) - mediated hospital infections in China prompted a need to investigate the genetic basis of emergence of such strains. A nationwide survey was conducted in China covering a total of 1105 CRE strains collected from 25 geographical locales with results showing that acquisition of two carbapenemase genes, blaKPC-2 and blaNDM, was responsible for phenotypic resistance in 90% of the CRE strains tested (58% and 32% respectively), among which several major strain types, such as ST11 of K...
April 26, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28477103/design-synthesis-and-fluorescence-property-evaluation-of-blue-emitting-triazole-linked-chromene-peptidomimetics
#16
T Jency Mohan, D Bahulayan
A highly efficient "Click with MCR" strategy for the three-step synthesis of two types of blue emitting chromene peptidomimetics is described. The peptidomimetics were synthesized via a copper-catalyzed [3[Formula: see text]2] azide-alkyne cycloaddition between chromene alkynes obtained from a three-component reaction and the peptide azides obtained from Ugi or Mannich type multicomponent reactions. The photophysical properties of the peptidomimetics are comparable with commercial fluorophores. Computational studies using drug property descriptors support the possibility of using these molecules for modulating difficult target classes having large, flat, and groove-shaped binding sites...
May 6, 2017: Molecular Diversity
https://www.readbyqxmd.com/read/28467974/expression-of-sialyl-tn-sugar-antigen-in-bladder-cancer-cells-affects-response-to-bacillus-calmette-gu%C3%A3-rin-bcg-and-to-oxidative-damage
#17
Paulo F Severino, Mariana Silva, Mylene Carrascal, Nadia Malagolini, Mariella Chiricolo, Giulia Venturi, Annalisa Astolfi, Mariangela Catera, Paula A Videira, Fabio Dall'Olio
The sialyl-Tn (sTn) antigen is an O-linked carbohydrate chain aberrantly expressed in bladder cancer (BC), whose biosynthesis is mainly controlled by the sialyltransferase ST6GALNAC1. Treatment with Bacillus Calmette-Guérin (BCG) is the most effective adjuvant immunotherapy for superficial BC but one third of the patients fail to respond. A poorly understood correlation between the expression of sTn and BC patient's response to BCG was previously observed. By analyzing tumor tissues, we showed that patients with high ST6GALNAC1 and IL-6 mRNA expression were BCG responders...
April 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28458209/effect-of-naphtha-diluent-on-greenhouse-gases-and-reduced-sulfur-compounds-emissions-from-oil-sands-tailings
#18
Kathleen F Gee, Ho Yin Poon, Zaher Hashisho, Ania C Ulrich
The long-term storage of oil sands tailings has resulted in the evolution of greenhouse gases (CH4 and CO2) as a result of residual organics biodegradation. Recent studies have identified black, sulfidic zones below the tailings-water interface, which may be producing toxic sulfur-containing gases. An anaerobic mesocosm study was conducted over an 11-week period to characterize the evolution of CH4, CO2 and reduced sulfur compounds (RSCs) (including H2S) in tailings as it relates to naphtha-containing diluent concentrations (0...
April 27, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28449732/three-cases-of-mcr-1-positive-colistin-resistant-escherichia-coli-bloodstream-infections-in-italy-august-2016-to-january-2017
#19
Marta Corbella, Bianca Mariani, Carolina Ferrari, Francesco Comandatore, Erika Scaltriti, Piero Marone, Patrizia Cambieri
We describe three cases of bloodstream infection caused by colistin-resistant Escherichia coli in patients in a tertiary hospital in Italy, between August 2016 and January 2017. Whole genome sequencing detected the mcr-1 gene in three isolated strains belonging to different sequence types (STs). This occurrence of three cases with mcr-1-positive E. coli belonging to different STs in six months suggests a widespread problem in settings where high multidrug resistance is endemic such as in Italy.
April 20, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28438944/genomic-insights-into-mcr-1-positive-plasmids-carried-by-the-colistin-resistant-escherichia-coli-from-the-inpatients
#20
Qingjing Wang, Jian Sun, Youfa Ding, Xing-Ping Li, Ya-Hong Liu, Youjun Feng
Colistin, a family of cationic polypeptide antibiotics, acts as a final resort against infections by the multidrug-resistant Gram-negative pathogens.….
April 24, 2017: Antimicrobial Agents and Chemotherapy
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