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https://www.readbyqxmd.com/read/28212517/phytoremediation-of-dyes-using-lagerstroemia-speciosa-mediated-silver-nanoparticles-and-its-biofilm-activity-against-clinical-strains-pseudomonas-aeruginosa
#1
V Sai Saraswathi, Neethu Kamarudheen, K V BhaskaraRao, K Santhakumar
The aim of this study was to prepare silver nanoparticles by a green method using the aqueous leaves extract of Lagerstroemia speciosa. The prepared silver nanoparticles were characterized, studied for its photocatalytic and biofilm inhibition studies. The maximum absorbance peak was found at 427nm and thus confirming the formation of silver nanoparticles. The average size of silver nanoparticles synthesized was found to be 12nm using XRD and it was spherical in shape. The nanoparticles synthesized was investigated for photocatalytic activity for to two different dye molecules, methyl orange and methylene blue showing 310 and 290min degradation time respectively...
February 10, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28204863/influence-of-twitching-and-swarming-motilities-on-biofilm-formation-in-pseudomonas-strains
#2
Letícia Muner Otton, Marina da Silva Campos, Karine Lena Meneghetti, Gertrudes Corção
The genus Pseudomonas mainly includes opportunistic pathogens that rely on type IV pili as an important virulence factor, which is associated with adherence and biofilm formation. Pseudomonas infections are well known to be persistent and resilient in nature largely because of the tendency of the species to form biofilms. This study aimed at analyzing environmental strains of Pseudomonas genus with respect to their ability to execute twitching and swarming motilities as well as with respect to their ability to form biofilms both in the presence as well as in the absence of furanone, a substance that has the potential to prevent the formation of biofilms...
February 15, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28196974/the-efficacy-of-different-anti-microbial-metals-at-preventing-the-formation-of-and-eradicating-bacterial-biofilms-of-pathogenic-indicator-strains
#3
Natalie Gugala, Joe A Lemire, Raymond J Turner
The emergence of multidrug-resistant pathogens and the prevalence of biofilm-related infections have generated a demand for alternative anti-microbial therapies. Metals have not been explored in adequate detail for their capacity to combat infectious disease. Metal compounds can now be found in textiles, medical devices and disinfectants-yet, we know little about their efficacy against specific pathogens. To help fill this knowledge gap, we report on the anti-microbial and antibiofilm activity of seven metals: silver, copper, titanium, gallium, nickel, aluminum and zinc against three bacterial strains, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli...
February 15, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28188877/laser-deposition-of-poly-3-hydroxybutyric-acid-co-3-hydroxyvaleric-acid-lysozyme-microspheres-based-coatings-with-anti-microbial-properties
#4
V Grumezescu, A M Holban, L E Sima, M B Chiritoiu, G N Chiritoiu, A M Grumezescu, L Ivan, F Safciuc, F Antohe, C Florica, C R Luculescu, M C Chifiriuc, G Socol
The purpose of this study was to obtain, characterize and evaluate the cytotoxicity and antimicrobial activity of coatings based on poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) - Lysozyme (P(3HB-3HV)/Lys) and P(3HB-3HV) - Polyethylene glycol - Lysozyme (P(3HB-3HV)/PEG/Lys) spheres prepared by Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique, in order to obtain functional and improved Ti-based implants. Morphological investigation of the coatings by Infrared Microscopy (IRM) and SEM revealed that the average diameter of P(3HB-3HV)/Lys spheres is around 2μm and unlike the drop cast samples, IRM recorded on MAPLE films revealed a good distribution of monitored functional groups on the entire scanned surface...
February 7, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28188589/synergistic-activity-of-sub-inhibitory-concentrations-of-curcumin-with-ceftazidime-and-ciprofloxacin-against-pseudomonas-aeruginosa-quorum-sensing-related-genes-and-virulence-traits
#5
Shekoufeh Roudashti, Habib Zeighami, Hesam Mirshahabi, Shahin Bahari, Ali Soltani, Fakhri Haghi
Quorum sensing (QS) system in Pseudomonas aeruginosa may be an important target for pharmacological intervention. The present study aimed to investigate the synergetic activity of sub-MIC concentrations of curcumin (C) with ceftazidime (CAZ) and ciprofloxacin (CIP) against P. aeroginusa QS system. We determined the MIC and synergistic activity of C, CAZ and CIP against P. aeroginusa PAO1 using broth microdilution and checkerboard titration methods. The activity of sub-MIC (1/4 and 1/16 MIC) concentrations of C on the QS signal molecules was assessed using a reporter strain assay...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28187935/effect-of-bacteria-on-the-degradation-ability-of-pleurotus-ostreatus
#6
Hana Válková, Čeněk Novotný, Kateřina Malachová, Pavlína Šlosarčíková, Jindřich Fojtík
White-rot fungi are efficient degraders of lignin whose extracellular enzymes have a potential to degrade organopollutants. In natural conditions these fungi enter into interactions with other organisms, which may affect their biodegradation capacity. The aim was to investigate the ability of Pleurotus ostreatus to form stable biofilms and to test the capacity of the fungus to degrade Remazol Brilliant Blue R in mixed cultures with bacteria. Bacterial counts were determined to see the behavior of the bacterium in the mixed culture with the fungus...
February 7, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28186120/dimerisation-induced-formation-of-the-active-site-and-the-identification-of-three-metal-sites-in-eal-phosphodiesterases
#7
Dom Bellini, Sam Horrell, Andrew Hutchin, Curtis W Phippen, Richard W Strange, Yuming Cai, Armin Wagner, Jeremy S Webb, Ivo Tews, Martin A Walsh
The bacterial second messenger cyclic di-3',5'-guanosine monophosphate (c-di-GMP) is a key regulator of bacterial motility and virulence. As high levels of c-di-GMP are associated with the biofilm lifestyle, c-di-GMP hydrolysing phosphodiesterases (PDEs) have been identified as key targets to aid development of novel strategies to treat chronic infection by exploiting biofilm dispersal. We have studied the EAL signature motif-containing phosphodiesterase domains from the Pseudomonas aeruginosa proteins PA3825 (PA3825(EAL)) and PA1727 (MucR(EAL))...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28173753/conjugative-type-ivb-pilus-recognizes-lipopolysaccharide-of-recipient-cells-to-initiate-papi-1-pathogenicity-island-transfer-in-pseudomonas-aeruginosa
#8
Toan Phuoc Hong, Michelle Q Carter, Paolo Struffi, Stefano Casonato, Youai Hao, Joseph S Lam, Stephen Lory, Olivier Jousson
BACKGROUND: Pseudomonas aeruginosa pathogenicity island 1 (PAPI-1) is one of the largest genomic islands of this important opportunistic human pathogen. Previous studies have shown that PAPI-1 encodes several putative virulence factors, including a major regulator of biofilm formation and antibiotic-resistance traits. PAPI-1 is horizontally transferable into recipient strains lacking this island via conjugation mediated by the specialized type IV pilus. The PAPI-1 encodes a cluster of ten genes associated with the synthesis and assembly of the type IV pilus...
February 7, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28167524/gas-sensing-and-signaling-in-the-pas-heme-domain-of-the-pseudomonas-aeruginosa-aer2-receptor
#9
Darysbel Garcia, Emilie Orillard, Mark S Johnson, Kylie J Watts
: The Aer2 chemoreceptor from Pseudomonas aeruginosa contains a PAS sensing domain that coordinates b-type heme and signals in response to the binding of O2, CO or NO. PAS-heme structures suggest that Aer2 uniquely coordinates heme via a His residue on a 310 helix (H234 on Eη), stabilizes O2 binding via a Trp residue (W283), and signals via both W283 and an adjacent Leu residue (L264). Ligand binding may displace L264 and reorient W283 for hydrogen-bonding to the ligand. Here we clarified the mechanisms by which Aer2-PAS binds heme, regulates ligand-binding, and initiates conformational signaling...
February 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28167523/the-role-of-cyclic-di-gmp-and-exopolysaccharide-in-type-iv-pilus-dynamics
#10
Jan Ribbe, Amy E Baker, Sebstian Euler, George A O'Toole, Berenike Maier
For Pseudomonas aeruginosa the levels of cyclic di-GMP govern the transition from the planktonic state to biofilm formation. Type IV pili (T4P) are crucial determinants of biofilm structure and dynamics, but it is unknown how the levels of c-di-GMP affect pilus dynamics. Here, we scrutinized how c-di-GMP affects molecular motor properties and adhesive behavior of T4P. By means of retraction, T4P generated forces of ∼ 30 pN. Deletion mutants in the proteins with known roles in biofilm formation, swarming motility and exopolysaccharide (EPS) production, specifically, the diguanulate cyclases sadC and roeA or the c-di-GMP phosphodiesterase bifA, showed only modest effects on velocity or force of T4P retraction...
February 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28166091/oral-polyphosphate-suppresses-bacterial-collagenase-production-and-prevents-anastomotic-leak-due-to-serratia-marcescens-and-pseudomonas-aeruginosa
#11
Sanjiv K Hyoju, Robin E Klabbers, Melissa Aaron, Monika A Krezalek, Alexander Zaborin, Mara Wiegerinck, Neil H Hyman, Olga Zaborina, Harry Van Goor, John C Alverdy
OBJECTIVE: The objective of this study was to determine the effect of polyphosphate on intestinal bacterial collagenase production and anastomotic leak in mice undergoing colon surgery. BACKGROUND: We have previously shown that anastomotic leak can be caused by intestinal pathogens that produce collagenase. Because bacteria harbor sensory systems to detect the extracellular concentration of phosphate which controls their virulence, we tested whether local phosphate administration in the form of polyphosphate could attenuate pathogen virulence and prevent leak without affecting bacterial growth...
February 3, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/28163960/screening-for-antibacterial-and-antibiofilm-activities-in-astragalus-angulosus
#12
Hussein Kanaan, Majid El-Mestrah, Alaa Sweidan, Falah As-Sadi, Ali Al Bazzal, Ali Chokr
AIM: In a search for finding novel therapeutic agents, extracts from an endemic Lebanese plant, Astragalus angulosus, were evaluated for their potential in-vitro antibacterial and antibiofilm activities against three Gram-positive bacterial strains; Staphylococcus epidermidis (CIP444), Staphylococcus aureus (ATCC25923), and Enterococcus faecalis (ATCC29212); in addition to two Gram-negative strains, Escherichia coli (ATCC35218) and Pseudomonas aeruginosa (ATCC27853). MATERIALS AND METHODS: The plant was collected in April of 2013 and divided into several different portions, then its extracts were obtained by maceration using two different solvents...
January 2017: Journal of Intercultural Ethnopharmacology
https://www.readbyqxmd.com/read/28161488/cold-atmospheric-pressure-plasma-elimination-of-clinically-important-single-and-mixed-species-biofilms
#13
Martina Modic, Neil P McLeod, J Mark Sutton, James L Walsh
Mixed-species biofilms reflect the natural environment of many pathogens in clinical settings and are highly resistant to disinfection methods. An indirect cold atmospheric-pressure air-plasma system was evaluated under two different discharge conditions for its ability to kill representative Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) pathogens. Plasma treatment of individual 24-h-old biofilms and mixed-species biofilms that contained additional species (Enterococcus faecalis and Klebsiella pneumoniae) was considered...
February 1, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28156000/rhamnolipids-from-pseudomonas-aeruginosa-strain-w10-as-antibiofilm-antibiofouling-products-for-metal-protection
#14
Alif Chebbi, Mohamed Elshikh, Farazul Haque, Syed Ahmed, Sara Dobbin, Roger Marchant, Sami Sayadi, Mohamed Chamkha, Ibrahim M Banat
Industrial biofouling-problems associated with the accumulation of microorganisms from flowing water and fluids on processing surfaces can cause severe problems. A Pseudomonas aeruginosa strain W10 was isolated from industrial setting and found to produce predominantly di-rhamnolipids (Rha-Rha-C10-C10) with a yield of around 10 g L(-1) and a critical micelle concentration (CMC) of 80 mg L(-1) . P. aeruginosa W10 rhamnolipids were able to disrupt up to 99% of 48 h pre-formed biofilms of the Gram-positive organisms Bacillus licheniformis CAN55, Staphylococcus capitis SH6, and a mixed culture (strains CAN55, SH6, and W10), under static conditions, at concentrations of 0...
February 3, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28155216/evaluation-of-the-role-of-substrate-and-albumin-on-pseudomonas-aeruginosa-biofilm-morphology-through-fesem-and-ftir-studies-on-polymeric-biomaterials
#15
S Dutta Sinha, Susmita Chatterjee, P K Maiti, S Tarafdar, S P Moulik
Bacterial biofilms pose the greatest challenge to implant surgeries leading to device-related infections and implant failure. Our present study aims at monitoring the variation in the biofilm architecture of a clinically isolated strain and ATCC 27853 strain of Pseudomonas aeruginosa on two polymeric biomaterials, used in implants. The perspective of our study is to recognize the potential of these two biomaterials to create biofilm infections and develop the understanding regarding their limitations of use and handle patients with this deeper insight...
February 2, 2017: Progress in biomaterials
https://www.readbyqxmd.com/read/28150937/nontoxic-cationic-coumarin-polyester-coatings-prevent-pseudomonas-aeruginosa-biofilm-formation
#16
Elaheh A Chamsaz, Steven Mankoci, Hazel A Barton, Abraham Joy
The rapid increase in bacterial infections and antimicrobial resistance is a growing public health concern. Infections arising from bacterial contamination of surgical tools, medical implants, catheters, and hospital surfaces can potentially be addressed by antimicrobial polymeric coatings. The challenge in developing such polymers for in vivo use is the ability to achieve high antimicrobial efficacy while at the same time being nontoxic to human cells. Although several classes of antimicrobial polymers have been developed, many of them cannot be used in the clinical setting due to their nonselective toxicity toward bacteria and mammalian cells...
February 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28150115/green-synthesis-of-rifampicin-loaded-copper-nanoparticles-with-enhanced-antimicrobial-activity
#17
Marta J Woźniak-Budych, Łucja Przysiecka, Krzysztof Langer, Barbara Peplińska, Marcin Jarek, Maciej Wiesner, Grzegorz Nowaczyk, Stefan Jurga
The antimicrobial properties of copper and rifampicin-loaded copper nanoparticles were investigated using four strains: Staphylococcus aureus, Escherichia coli, Bacillus pumilis and Pseudomonas fluorescens. Spherical-shaped copper nanoparticles were synthesized via green reduction method from the peppermint extract. It was found that adsorption of rifampicin on the copper nanosurface enhances its biological activity and prevents the development of resistance. The interactions between rifampicin-copper nanoparticles and bacteria cells were monitored using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM)...
March 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28149169/anti-quorum-sensing-and-anti-biofilm-activity-of-amomum-tsaoko-amommum-tsao-ko-crevost-et-lemarie-on-foodborne-pathogens
#18
Md Ramim Tanver Rahman, Zaixiang Lou, Fuhao Yu, Peng Wang, Hongxin Wang
Cell-to-cell communication or quorum sensing (QS) leads to biofilm formation and causing other virulence factors which are extreme problems for food safety, biofilm related infectious diseases etc. This study evaluated the anti-QS activity of the Amomum tsaoko extract (0.5-4 mg/ml) by using Chromobacterium violaceum a biosensor strain and biofilm formation by crystal violate assay. Experimental results demonstrated that the overall yield of Amomum tsao-ko extract was 11.33 ± 0.3% (w/w). MIC for Staphylococcus aureus (Gram positive), Salmonella Typhimurium and Pseudomonas aeruginosa (Gram negative) was 1, 2 and 2 mg/ml, respectively...
February 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28147485/hydrodynamic-effects-on-biofilms-at-the-biointerface-using-a-microfluidic-electrochemical-cell-case-study-of-pseudomonas-sp
#19
Mir Pouyan Zarabadi, François Paquet-Mercier, Steve J Charette, Jesse Greener
The anchoring biofilm layer is expected to exhibit a different response to environmental stresses than for portions in the bulk, due to the protection from other strata and the proximity to the attachment surface. The effect of hydrodynamic stress on surface-adhered biofilm layers was tested using a specially designed microfluidic bio flow cell with an embedded three-electrode detection system. In situ electrochemical impedance spectroscopy (EIS) measurements of biocapacitance and bioresistance of Pseudomonas sp...
February 14, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28144921/comparative-transcriptome-analysis-of-trueperella-pyogenes-reveals-a-novel-antimicrobial-strategy
#20
Kelei Zhao, Wujiao Li, Ting Huang, Xuhao Song, Xiuyue Zhang, Bisong Yue
Trueperella pyogenes is a prevalent opportunistic bacterium that normally causes diverse suppurative lesions, endometritis and pneumonia in various economically important animals. Although the genomic information of this species has been announced, little is known about its functional profiles. In this study, by performing a comparative transcriptome analysis between the highly and moderately virulent T. pyogenes isolates, we found the expression of a LuxR-type DNA-binding response regulator, PloR, was significantly up-regulated in the highly virulent T...
January 31, 2017: Archives of Microbiology
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