keyword
MENU ▼
Read by QxMD icon Read
search

pseudomonas biofilm

keyword
https://www.readbyqxmd.com/read/29909325/a-novel-microfluidic-system-enables-visualization-and-analysis-of-antibiotic-resistance-gene-transfer-to-activated-sludge-bacteria-in-biofilm
#1
Yong Qiu, Jing Zhang, Bing Li, Xianghua Wen, Peng Liang, Xia Huang
Antibiotic resistance genes (ARGs) in environment have become a growing public concern, due to their potential to be obtained by pathogens and their duplication along cell division. Horizontal gene transfer (HGT) was reported to be responsible for ARGs dissemination in microbes, but the HGT feature in environmental biofilm was still unclear due to insufficient assay tools. To address this challenge, we applied a novel microfluidic system to cultivate thin biofilm by continuous supply of nutrients and close contact between cells...
June 14, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29902594/characterization-of-clinically-relevant-model-bacterial-strains-of-pseudomonas-aeruginosa-for-anti-biofilm-testing-of-materials
#2
Olena Rzhepishevska, Nataliia Limanska, Mykola Galkin, Alicia Lacoma, Margaretha Lundquist, Dmytro Sokol, Shoghik Hakobyan, Anders Sjöstedt, Cristina Prat, Madeleine Ramstedt
There is a great interest in developing novel anti-biofilm materials in order to decrease medical device-associated bacterial infections causing morbidity and high healthcare costs. However, the testing of novel materials is often done using bacterial lab strains that may not exhibit the same phenotype as clinically relevant strains infecting medical devices. Furthermore, no consensus of strain selection exists in the field, making results very difficult to compare between studies. In this work, 19 clinical isolates of Pseudomonas aeruginosa originating from intubated patients in an intensive care unit have been characterized and compared to the lab reference strain PAO1 and a rmlC lipopolysaccharide mutant of PAO1...
June 11, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29902223/modelling-of-ciprofloxacin-killing-enhanced-by-hyperbaric-oxygen-treatment-in-pseudomonas-aeruginosa-pao1-biofilms
#3
Peter Alexander Vistar Gade, Terkel Bo Olsen, Peter Østrup Jensen, Mette Kolpen, Niels Høiby, Kaj-Åge Henneberg, Thomas Sams
OUTLINE: In chronic lung infections by Pseudomonas aeruginosa (PA) the bacteria thrive in biofilm structures protected from the immune system of the host and from antibiotic treatment. Increasing evidence suggests that the susceptibility of the bacteria to antibiotic treatment can be significantly enhanced by hyperbaric oxygen treatment. The aim of this study is to simulate the effect of ciprofloxacin treatment in a PAO1 biofilm model with aggregates in agarose when combined with hyperbaric oxygen treatment...
2018: PloS One
https://www.readbyqxmd.com/read/29901811/decreased-susceptibility-of-streptococcus-anginosus-to-vancomycin-in-a-multispecies-biofilm-is-due-to-increased-thickness-of-the-cell-wall
#4
Sarah Tavernier, Andrea Sass, Michiel De Bruyne, Femke Baeke, Riet De Rycke, Aurélie Crabbé, Ilse Vandecandelaere, Filip Van Nieuwerburgh, Tom Coenye
Background: Streptococcus anginosus, Pseudomonas aeruginosa and Staphylococcus aureus are often co-isolated from the sputum of cystic fibrosis patients. It was recently shown that S. anginosus is protected from the activity of vancomycin when it grows in a multispecies biofilm with P. aeruginosa and S. aureus. Objectives: Elucidating the underlying cause of the reduced susceptibility of S. anginosus to vancomycin when growing in a multispecies biofilm with P. aeruginosa and S...
June 12, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29898603/role-of-ionic-strength-in-the-retention-and-initial-attachment-of-pseudomonas-putida-to-quartz-sand
#5
Somayeh Ramezanian, Hien Xuan Ta, Balasingam Muhunthan, Nehal Abu-Lail
Biofilms in soil can offer solutions to many geotechnical problems. Controlling biofilm formation in soil requires a fundamental understanding of how bacterial cells interact with soil under relevant environmental conditions. Here, atomic force microscopy (AFM) was used to quantify the adhesion forces acting between a model soil microbe, Pseudomonas putida, and a model of quartz sand under a range of ionic strengths (ISs; 0.02M-0.52M) that bracket common environmental conditions present in soil. AFM forces were decoupled into specific and nonspecific components following the established Poisson statistical analysis method...
June 13, 2018: Biointerphases
https://www.readbyqxmd.com/read/29894900/enhancing-extracellular-electron-transfer-between-pseudomonas-aeruginosa-pao1-and-light-driven-semiconducting-birnessite
#6
Guiping Ren, Yuan Sun, Yang Ding, Anhuai Lu, Yan Li, Changqiu Wang, Hongrui Ding
In recent years, considerable research effort has explored the interaction between semiconducting minerals and microorganisms, such relationship is a promising way to increase the efficiency of bioelectrochemical systems. Herein, the enhancement of electron transfer between birnessite photoanodes and Pseudomonas aeruginosa PAO1 under visible light was investigated. Under light illumination and positive bias, the light-birnessite-PAO1 electrochemical system generated a photocurrent of 279.57 μA/cm2 , which is 322% and 170% higher than those in the abiotic control and dead culture, suggesting photoenhanced electrochemical interaction between birnessite and Pseudomonas...
June 2, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29890626/cobalt-complex-with-thiazole-based-ligand-as-new-pseudomonas-aeruginosa-quorum-quencher-biofilm-inhibitor-and-virulence-attenuator
#7
Anabela Borges, Manuel Simões, Tamara R Todorović, Nenad R Filipović, Alfonso T García-Sosa
Pseudomonas aeruginosa is one of the most dreaded human pathogens, because of its intrinsic resistance to a number of commonly used antibiotics and ability to form sessile communities (biofilms). Innovative treatment strategies are required and that can rely on the attenuation of the pathogenicity and virulence traits. The interruption of the mechanisms of intercellular communication in bacteria (quorum sensing) is one of such promising strategies. A cobalt coordination compound (Co( HL )₂) synthesized from ( E )-2-(2-(pyridin-2-ylmethylene)hydrazinyl)-4-(p-tolyl)thiazole ( HL ) is reported herein for the first time to inhibit P...
June 8, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29888590/food-safe-modification-of-stainless-steel-food-processing-surfaces-to-reduce-bacterial-biofilms
#8
Tarek Samir Awad, Dalal Asker, Benjamin D Hatton
Biofilm formation on stainless steel (SS) surfaces of food processing plants, leading to foodborne illness outbreaks, is enabled by the attachment and confinement within microscale cavities of surface roughness (grooves, scratches). We report Foodsafe Oil-based Slippery Coatings (FOSCs) for food processing surfaces that suppress bacterial adherence and biofilm formation by trapping residual oil lubricant within these surface cavities to block microbial growth. SS surfaces were chemically functionalized with alkylphosphonic acid to preferentially wet a layer of food grade oil...
June 11, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29886364/effects-of-secondary-carbon-supplement-on-biofilm-mediated-biodegradation-of-naphthalene-by-mutated-naphthalene-1-2-dioxygenase-encoded-by-pseudomonas-putida-strain-kd9
#9
Kunal Dutta, Sergey Shityakov, Ibrahim Khalifa, Arpan Mal, Satya Priya Moulik, Amiya Kumar Panda, Chandradipa Ghosh
Polycyclic aromatic hydrocarbons (PAHs) belong to a diverse group of environmental pollutants distributed ubiquitously in the environment. The carcinogenic properties of PAHs are the main causes of harm to human health. The green technology, biodegradation have become convenient options to address the environmental pollution. In this study, we analyzed the biodegradation potential of naphthalene with secondary carbon supplements (SCSs) in carbon deficient media (CSM) by Pseudomonas putida strain KD9 isolated from oil refinerary waste...
May 18, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29886096/a-new-therapeutic-avenue-for-bronchiectasis-dry-powder-inhaler-of-ciprofloxacin-nanoplex-exhibits-superior-ex-vivo-mucus-permeability-and-antibacterial-efficacy-to-its-native-ciprofloxacin-counterpart
#10
The-Thien Tran, Celine Vidaillac, Hong Yu, Valerie F L Yong, Dan Roizman, Ravishankar Chandrasekaran, Albert Y H Lim, Teck Boon Low, Gan Liang Tan, John A Abisheganaden, Mariko Siyue Koh, Jeanette Teo, Sanjay H Chotirmall, Kunn Hadinoto
Non-cystic fibrosis bronchiectasis (NCFB) characterized by permanent bronchial dilatation and recurrent infections has been clinically managed by long-term intermittent inhaled antibiotic therapy among other treatments. Herein we investigated dry powder inhaler (DPI) formulation of ciprofloxacin (CIP) nanoplex with mannitol/lactose as the excipient for NCFB therapy. The DPI of CIP nanoplex was evaluated against DPI of native CIP in terms of their (1) dissolution characteristics in artificial sputum medium, (2) ex vivo mucus permeability in sputum from NCFB and healthy individuals, (3) antibacterial efficacy in the presence of sputum against clinical Pseudomonas aeruginosa strains (planktonic and biofilm), and (4) cytotoxicity towards human lung epithelial cells...
June 7, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29886016/the-fapf-amyloid-secretion-transporter-possesses-an-atypical-asymmetric-coiled-coil
#11
Sarah L Rouse, Fisentzos Stylianou, H Y Grace Wu, Jamie-Lee Berry, Lee Sewell, R Marc L Morgan, Andrea C Sauerwein, Steve Matthews
Gram-negative bacteria possess specialised biogenesis machineries that facilitate the export of amyloid subunits, the fibres of which are key components of their biofilm matrix. The secretion of bacterial functional amyloid requires a specialised outer-membrane protein channel through which unfolded amyloid substrates are translocated. We previously reported the crystal structure of the membrane-spanning domain of the amyloid subunit transporter FapF from Pseudomonas. However, the structure of the periplasmic domain, which is essential for amyloid transport, is yet to be determined...
June 7, 2018: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29885070/expression-and-regulation-of-the-pseudomonas-aeruginosa-hibernation-promoting-factor
#12
Tatsuya Akiyama, Kerry S Williamson, Michael J Franklin
Bacterial biofilms contain subpopulations of cells that are dormant and highly tolerant to antibiotics. While dormant, the bacteria must maintain the integrity of macromolecules required for resuscitation. Previously, we showed that hibernation promoting factor (HPF) is essential for protecting Pseudomonas aeruginosa from ribosomal loss during dormancy. In this study, we mapped the genetic components required for hpf expression. Using 5'-RACE and fluorescent protein reporter fusions, we show that hpf is expressed as part of the rpoN operon, but that hpf also has a second promoter (Phpf ) within the rpoN gene...
June 8, 2018: Molecular Microbiology
https://www.readbyqxmd.com/read/29883434/critical-assessment-of-methods-to-quantify-biofilm-growth-and-evaluate-antibiofilm-activity-of-host-defence-peptides
#13
Evan F Haney, Michael J Trimble, John T Cheng, Quentin Vallé, Robert E W Hancock
Biofilms are multicellular communities of bacteria that can adhere to virtually any surface. Bacterial biofilms are clinically relevant, as they are responsible for up to two-thirds of hospital acquired infections and contribute to chronic infections. Troublingly, the bacteria within a biofilm are adaptively resistant to antibiotic treatment and it can take up to 1000 times more antibiotic to kill cells within a biofilm when compared to planktonic bacterial cells. Identifying and optimizing compounds that specifically target bacteria growing in biofilms is required to address this growing concern and the reported antibiofilm activity of natural and synthetic host defence peptides has garnered significant interest...
May 21, 2018: Biomolecules
https://www.readbyqxmd.com/read/29879584/enhancement-of-bioelectricity-generation-via-heterologous-expression-of-irre-in-pseudomonas-aeruginosa-inoculated-mfcs
#14
Jianmei Luo, Tingting Wang, Xiao Li, Yanan Yang, Minghua Zhou, Ming Li, Zhongli Yan
Low electricity power output (EPT) is still the main bottleneck limited the industrial application of microbial fuel cells (MFCs). Herein, EPT enhancement by introducing an exogenous global regulator IrrE derived from Deinococcus radiodurans into electrochemically active bacteria (EAB) was explored using Pseudomonas aeruginosa PAO1 as a model strain, achieving a power density 71% higher than that of the control strain. Moreover, IrrE-expressing strain exhibited a remarkable increase in the total amount of electron shuttles (majorly phenazines compounds) and a little decrease in internal resistance, which should underlie the enhancement in extracellular electron transfer (EET) efficiency and EPT...
May 30, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29875759/pa0833-is-an-ompa-c-like-protein-that-confers-protection-against-pseudomonas-aeruginosa-infection
#15
Feng Yang, Jiang Gu, Jintao Zou, Langhuan Lei, Haiming Jing, Jin Zhang, Hao Zeng, Quanming Zou, Fenglin Lv, Jinyong Zhang
Pseudomonas aeruginosa is a formidable pathogen that causes infections with high mortality rates. Because of its ability to form biofilms and rapidly acquire resistance to many first-line antibiotics, P. aeruginosa -related infections are typically difficult to cure by traditional antibiotic treatment regimes. Thus, new strategies to prevent and treat such infections are urgently required. PA0833 is a newly identified protective antigen of P. aeruginosa that was identified in a screen using a reverse vaccine strategy in our laboratory...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29872101/terrein-is-an-inhibitor-of-quorum-sensing-and-c-di-gmp-in-pseudomonas-aeruginosa-a-connection-between-quorum-sensing-and-c-di-gmp
#16
Bomin Kim, Ji-Su Park, Ha-Young Choi, Sang Sun Yoon, Won-Gon Kim
To address the drug-resistance of bacterial pathogens without imposing a selective survival pressure, virulence and biofilms are highly attractive targets. Here, we show that terrein, which was isolated from Aspergillus terreus, reduced virulence factors (elastase, pyocyanin, and rhamnolipid) and biofilm formation via antagonizing quorum sensing (QS) receptors without affecting Pseudomonas aeruginosa cell growth. Additionally, the effects of terrein on the production of QS signaling molecules and expression of QS-related genes were verified...
June 5, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29867886/aerobic-vitamin-b-12-biosynthesis-is-essential-for-pseudomonas-aeruginosa-class-ii-ribonucleotide-reductase-activity-during-planktonic-and-biofilm-growth
#17
Anna Crespo, Núria Blanco-Cabra, Eduard Torrents
Pseudomonas aeruginosa is a major pathogenic bacterium in chronic infections and is a model organism for studying biofilms. P. aeruginosa is considered an aerobic bacterium, but in the presence of nitrate, it also grows in anaerobic conditions. Oxygen diffusion through the biofilm generates metabolic and genetic diversity in P. aeruginosa growth, such as in ribonucleotide reductase activity. These essential enzymes are necessary for DNA synthesis and repair. Oxygen availability determines the activity of the three-ribonucleotide reductase (RNR) classes...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29867883/synergy-of-sodium-nitroprusside-and-nitrate-in-inhibiting-the-activity-of-sulfate-reducing-bacteria-in-oil-containing-bioreactors
#18
Tekle T Fida, Johanna Voordouw, Maryam Ataeian, Manuel Kleiner, Gloria Okpala, Jaspreet Mand, Gerrit Voordouw
Sodium nitroprusside (SNP) disrupts microbial biofilms through the release of nitric oxide (NO). The actions of SNP on bacteria have been mostly limited to the genera Pseudomonas , Clostridium , and Bacillus . There are no reports of its biocidal action on sulfate-reducing bacteria (SRB), which couple the reduction of sulfate to sulfide with the oxidation of organic electron donors. Here, we report the inhibition and kill of SRB by low SNP concentrations [0.05 mM (15 ppm)] depending on biomass concentration...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29866808/type-1-does-the-two-step-type-1-secretion-substrates-with-a-functional-periplasmic-intermediate
#19
Timothy J Smith, Holger Sondermann, George A O'Toole
Bacteria have evolved several secretion strategies for polling and responding to environmental flux and insult. Of these, the type 1 secretion system (T1SS) is known to secrete an array of biologically diverse proteins - from small < 10 kDa bacteriocins to gigantic adhesins with a mass over 1 MDa. For the last several decades T1SS have been characterized as a one-step translocation strategy whereby the secreted substrate is transported directly into the extracellular environment from the cytoplasm with no periplasmic intermediate...
June 4, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29866805/rsmv-a-small-non-coding-regulatory-rna-in-pseudomonas-aeruginosa-that-sequesters-rsma-and-rsmf-from-target-mrnas
#20
Kayley H Janssen, Manisha R Diaz, Cindy J Gode, Matthew C Wolfgang, Timothy L Yahr
The Gram-negative opportunistic pathogen Pseudomonas aeruginosa has distinct genetic programs that favor either acute or chronic virulence gene expression. Acute virulence is associated with twitching and swimming motility, expression of a type III secretion system (T3SS), and the absence of alginate, Psl, or Pel polysaccharide production. Traits associated with chronic infection include growth as a biofilm, reduced motility, and expression of a type VI secretion system (T6SS). The Rsm post-transcriptional regulatory system plays important roles in the inverse control of phenotypes associated with acute and chronic virulence...
June 4, 2018: Journal of Bacteriology
keyword
keyword
113158
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"