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https://www.readbyqxmd.com/read/28822938/do-natural-biofilm-impact-nzvi-mobility-and-interactions-with-porous-media-a-column-study
#1
Marc Crampon, Jennifer Hellal, Christophe Mouvet, Guillaume Wille, Caroline Michel, Anke Wiener, Juergen Braun, Patrick Ollivier
Nanoparticles (NP) used as remediation agents for groundwater treatment may interact with biofilms naturally present, altering NP mobility and/or reactivity and thereby NP effectiveness. The influence of the presence of a multi species biofilm on the mobility of two types of zero-valent iron NP (nZVI; NANOFER 25S and optimized NANOFER STAR, NanoIron s.r.o. (Czech Republic)) was tested in laboratory experiments with columns mimicking aquifer conditions. Biofilms were grown in columns filled with sand in nitrate reducing conditions using groundwater from an industrial site as inoculum...
August 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28819217/anti-biofilm-effects-of-anthranilate-on-a-broad-range-of-bacteria
#2
Xi-Hui Li, Soo-Kyoung Kim, Joon-Hee Lee
Anthranilate, one of tryptophan degradation products has been reported to interfere with biofilm formation by Pseudomonas aeruginosa. Here, we investigated the effects of anthranilate on biofilm formation by various bacteria and the mechanisms responsible. Anthranilate commonly inhibited biofilm formation by P. aeruginosa, Vibrio vulnificus, Bacillus subtilis, Salmonella enterica serovar Typhimurium, and Staphylococcus aureus, and disrupted biofilms preformed by these bacteria. Because anthranilate reduced intracellular c-di-GMP and enhanced swimming and swarming motilities in P...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28818490/inhibition-of-quorum-sensing-controlled-biofilm-formation-in-pseudomonas-aeruginosa-by-quorum-sensing-inhibitors
#3
Kalaiarasan Ellappan, Kottha Thirumalaswamy, Belgode Narasimha Harish, Gnanasambandam Vasuki, Veeresh Kumar Sali, James John
Antimicrobial therapy against extensively drug-resistant (XDR) P. aeruginosa biofilms is less efficient compared to the treatment of equal bacterial counts of free-floating planktonic cells, which has become a serious threat in hospital environment. P. aeruginosa regulate their cooperative activities and physiological process through a cell to cell chemical communication process called Quorum sensing (QS). This attracted our interest to synthesize, and to chemically characterize two anti-QS compounds, N-(4-{4-fluoroanilno} butanoyl) -L-homoserine lactone (FABHL) and N-(4-{4-chlororoanilno} butanoyl) -L-homoserine lactone (CABHL) to inhibit biofilm formation via disabling the QS circuits...
August 14, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28812948/age-related-resistance-in-arabidopsis-thaliana-involves-the-mads-domain-transcription-factor-short-vegetative-phase-and-direct-action-of-salicylic-acid-on-pseudomonas-syringae
#4
Daniel C Wilson, Christine J Kempthorne, Philip Carella, David K Liscombe, Robin Cameron
Arabidopsis thaliana exhibits a developmentally regulated disease-resistance response known as Age-Related Resistance (ARR), a process that requires intercellular accumulation of salicylic acid (SA), which is thought to act as an antimicrobial agent. ARR is characterized by enhanced resistance to some pathogens at the late adult-vegetative and reproductive stages. While the transition to flowering does not cause the onset of ARR, both processes involve the MADS-domain transcription factor SHORT VEGETATIVE PHASE (SVP)...
August 16, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28812768/chemical-nutritive-composition-and-a-wide-range-of-bioactive-properties-of-honey-mushroom-armillaria-mellea-vahl-fr-kummer
#5
Marina Kostić, Marija Smiljković, Jovana Petrović, Jasmina Glamočlija, Lillian Barros, Isabel C F R Ferreira, Ana Ćirić, Marina Soković
A. mellea fruiting bodies collected from nature were chemically characterized and shown to be rich in carbohydrates (81.25 g per 100 g dw), ash, fat and proteins (8.84 g per 100 g dw, 1.97 g per 100 g dw and 1.81 g per 100 g dw, respectively). Mannitol was the main free sugar while malic acid was the most abundant organic acid. δ-Tocopherol was the dominant form of tocopherols with 42.41 μg per 100 g dw. Polyunsaturated fatty acids were predominant, followed by saturated and monounsaturated fatty acids. A methanolic extract prepared from these samples was tested for antioxidant, quorum sensing and antimicrobial assays, as well as for its cytotoxicity effects...
August 16, 2017: Food & Function
https://www.readbyqxmd.com/read/28811582/a-new-class-of-hybrid-secretion-system-is-employed-in-pseudomonas-amyloid-biogenesis
#6
Sarah L Rouse, William J Hawthorne, Jamie-Lee Berry, Dror S Chorev, Sandra A Ionescu, Sebastian Lambert, Fisentzos Stylianou, Wiebke Ewert, Uma Mackie, R Marc L Morgan, Daniel Otzen, Florian-Alexander Herbst, Per H Nielsen, Morten Dueholm, Hagan Bayley, Carol V Robinson, Stephen Hare, Stephen Matthews
Gram-negative bacteria possess specialised biogenesis machineries that facilitate the export of amyloid subunits for construction of a biofilm matrix. The secretion of bacterial functional amyloid requires a bespoke outer-membrane protein channel through which unfolded amyloid substrates are translocated. Here, we combine X-ray crystallography, native mass spectrometry, single-channel electrical recording, molecular simulations and circular dichroism measurements to provide high-resolution structural insight into the functional amyloid transporter from Pseudomonas, FapF...
August 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/28810631/hyperoside-inhibits-biofilm-formation-of-pseudomonas-aeruginosa
#7
Yixuan Sun, Fengjun Sun, Wei Feng, Xuewen Qiu, Yao Liu, Bo Yang, Yongchuan Chen, Peiyuan Xia
Pseudomonas aeruginosa (P. aeruginosa) is a common pathogen in hospital-acquired infection and is readily able to form biofilms. Due to its high antibiotic resistance, traditional antibacterial treatments exert a limited effect on P. aeruginosa biofilm infections. It has been indicated that hyperoside inhibits P. aeruginosa PAO1 (PAO1) biofilm formation without affecting growth. Therefore, the current study examined the biofilm formation and quorum sensing (QS) system of PAO1 in the presence of hyperoside. Confocal laser scanning microscopy analysis demonstrated that hyperoside significantly inhibited biofilm formation...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28810610/microbial-investigations-in-throat-swab-and-tracheal-aspirate-specimens-are-beneficial-to-predict-the-corresponding-endotracheal-tube-biofilm-flora-among-intubated-neonates-with-ventilator-associated-pneumonia
#8
Yun Pan, Lizhong Du, Qing Ai, Sijie Song, Xiaoli Tang, Danping Zhu, Jialin Yu
Ventilator-associated pneumonia (VAP) is a common nosocomial infection in neonatal intensive care units with high morbidity and mortality. Bacterial biofilm in the endotracheal tube (ET) provides a notable and persistent source of pathogens that may cause VAP, and thus is important for VAP detection. However, during intubation microbial investigations in ET, samples are unavailable due to the infeasibility of collecting ET samples during intubation of neonates. It is therefore of great importance to find alternative sources of samples that can help identify the ET biofilm flora...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28804019/pseudomonas-aeruginosa-auto-inducer3-oxo-c12-hsl-exerts-bacteriostatic-effect-and-inhibits-staphylococcus-epidermidis-biofilm
#9
Pradeep Kumar Singh, Vivek Kumar Yadav, Manmohit Kalia, Suhaga Dohare, Deepmala Sharma, Vishnu Agarwal
Pseudomonas aeruginosa has evolved the 3-oxo-C12-HSL and C4-HSL based quorum sensing system which is responsible for the regulation of various virulence factors and helps to dominates over other bacterial species. Staphylococcus epidermidis has frequently been reported with P. aeruginosa while the role of C4-HSL and 3-oxo-C12-HSL on the S. epidermidis had widely been unexplored, and as per our knowledge, this is the first report on the impact of C4-HSL and 3-oxo-C12-HSL overS. epidermidis growth and biofilm...
August 10, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28804018/anti-biofilm-activity-of-a22-s-3-4-dichlorobenzyl-isothiourea-hydrochloride-against-pseudomonas-aeruginosa-influence-on-biofilm-formation-motility-and-bioadhesion
#10
Pauline Cordenonsi Bonez, Grazielle Guidolin Rossi, Jardel Rodrigo Bandeira, Andiara Prates Ramos, Caren Rigon Mizdal, Vanessa Albertina Agertt, Eloísa Salete Segatto Dalla Nora, Márcia Ebling de Souza, Camilla Fillippi Dos Santos Alves, Fallon Siqueira Dos Santos, André Gündel, Rodrigo de Almeida Vaucher, Roberto Christ Vianna Santos, Marli Matiko Anraku de Campos
Bacterial biofilms are involved in various medical infections and for this reason it is of great importance understanding adhesion mechanisms of involved microorganisms is essential to develop new strategies of prevention and control. Different approaches have been used for preventing biofilm related infections in health care settings, such as use of surface coatings agents in medical implants. In this context, is necessary to explore new compounds with anti-biofilm activity. Thus, this study evaluated for the first time the action of A22 against biofilms of Pseudomonas aeruginosa PAO1 strain and multi-resistant clinical isolates on biotic and abiotic surfaces...
August 10, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28802176/a-rapid-and-simple-protocol-to-prepare-a-living-biocomposite-that-mimics-electroactive-biofilms
#11
Stéphane Pinck, Mathieu Etienne, Manuel Dossot, Frédéric P A Jorand
A living material was formed by self-assembly of bacterial cells (Shewanella oneidensis MR-1 or Pseudomonas fluorescens) with carbon nanotubes in the presence of cytochrome c from a bovine heart with the goal to mimic electroactive biofilms. The role of cytochrome c on self-assembly, cell viability and extracellular electron transfer was studied. Scanning electron microscopy and dynamic light scattering experiments highlighted its role on the self-assembly of bacteria‑carbon nanotube aggregates within only 2h in solution...
August 1, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28801691/a-new-data-processing-routine-facilitating-the-identification-of-surface-adhered-proteins-from-bacterial-conditioning-films-via-qcm-d-maldi-tof-ms
#12
Siegfried Hohmann, Anke Neidig, Boris Kühl, Frank Kirschhöfer, Jörg Overhage, Gerald Brenner-Weiß
Conditioning films are an important factor in the initiation and development of microbial biofilms, which are the leading cause of chronic infections associated with medical devices. Here, we analyzed the protein content of conditioning films formed after exposure to supernatants of cultures of the human pathogen Pseudomonas aeruginosa PAO1. Adhesion of substances from the supernatant was monitored using quartz crystal microbalance with dissipation monitoring (QCM-D) sensor chips modified with the commonly used implant material titanium dioxide (TiO2)...
August 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28799693/the-small-rna-real-a-novel-regulatory-element-embedded-in-the-pseudomonas-aeruginosa-quorum-sensing-networks
#13
Sara Carloni, Raffaella Macchi, Sara Sattin, Silvia Ferrara, Giovanni Bertoni
The small RNA ReaL of the opportunistic pathogen Pseudomonas aeruginosa has been characterized. Our results indicate that ReaL contributes to P. aeruginosa virulence. In the Galleria mellonella infection model, reaL gene deletion resulted in decreased virulence, while ReaL overexpression resulted in a hyper-virulent phenotype. We also demonstrate that ReaL is embedded in the P. aeruginosa quorum sensing (QS) with the role of linking las to pqs systems. We show that ReaL is negatively regulated by the las regulator LasR and impacts positively the synthesis of the pqs quinolone signal PQS by a positive post-transcriptional effect on the pqsC gene...
August 11, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28798903/anti-quorum-sensing-and-anti-biofilm-activity-of-delftia-tsuruhatensis-extract-by-attenuating-the-quorum-sensing-controlled-virulence-factor-production-in-pseudomonas-aeruginosa
#14
Vijay K Singh, Avinash Mishra, Bhavanath Jha
Multidrug-resistance bacteria commonly use cell-to-cell communication that leads to biofilm formation as one of the mechanisms for developing resistance. Quorum sensing inhibition (QSI) is an effective approach for the prevention of biofilm formation. A Gram-negative bacterium, Delftia tsuruhatensis SJ01, was isolated from the rhizosphere of a species of sedge (Cyperus laevigatus) grown along the coastal-saline area. The isolate SJ01 culture and bacterial crude extract showed QSI activity in the biosensor plate containing the reference strain Chromobacterium violaceum CV026...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28798773/planktonic-growth-of-pseudomonas-aeruginosa-around-a-dual-species-biofilm-supports-the-growth-of-fusobacterium-nucleatum-within-that-biofilm
#15
James C Wang, Joehassin Cordero, Yan Sun, Mayank Aranke, Randall Wolcott, Jane A Colmer-Hamood, Abdul N Hamood
PURPOSE: The goal of this study was to understand the potential interaction between Pseudomonas aeruginosa and Fusobacterium nucleatum within the middle ear. METHODS: We examined the microbiota of ear fluid and tympanostomy tubes (TTs) obtained from patients with posttympanostomy tube otorrhea. We also examined biofilms formed by P. aeruginosa and F. nucleatum, singly or together, under aerobic or anaerobic conditions. RESULTS: While the facultative anaerobe P...
2017: International Journal of Otolaryngology
https://www.readbyqxmd.com/read/28797964/enhancing-biodegradation-of-c16-alkyl-quaternary-ammonium-compounds-using-an-oxygen-based-membrane-biofilm-reactor
#16
YenJung Sean Lai, Aura Ontiveros-Valencia, Zehra Esra Ilhan, Yun Zhou, Evelyn Miranda, Juan Maldonado, Rosa Krajmalnik-Brown, Bruce E Rittmann
Quaternary ammonium compounds (QACs) (e.g., hexadecyltrimethyl-ammonium bromide, CTAB) are emerging contaminants with widespread use as surfactants and disinfectants. Because the initial step of QAC biodegradation is mono-oxygenation, QAC degraders require O2, but normal aeration leads to serious foaming. Here, we developed and tested an oxygen-based membrane biofilm reactor (O2-MBfR) that delivers O2 by diffusion through the walls of hollow-membranes to a biofilm accumulating on the outer surface of membranes...
July 2, 2017: Water Research
https://www.readbyqxmd.com/read/28797773/methyl-propiolate-and-3-butynone-starting-points-for-synthesis-of-amphiphilic-1-2-3-triazole-peptidomimetics-for-antimicrobial-evaluation
#17
Thomas A Bakka, Morten B Strøm, Jeanette H Andersen, Odd R Gautun
A library of 29 small 1,4-substituted 1,2,3-triazoles was prepared for studies of antimicrobial activity. The pharmacophore model investigated with these substrates was based on small peptidomimetics of antimicrobial peptides and antimicrobials isolated from marine organisms from sub-arctic regions. Using methyl 1,2,3-triazole-carboxylates and 1,2,3-triazole methyl ketones prepared through "click" chemistry we were able to synthesize the different cationic amphiphiles through three steps or less. Several structural modifications to the lipopohilic side and hydrophilic sides of the amphiphiles were investigated and compared with regards to antimicrobial activity and cytotoxicity in particular...
July 29, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28792484/hospital-drains-as-reservoirs-of-pseudomonas-aeruginosa-multiple-locus-variable-number-of-tandem-repeats-analysis-genotypes-recovered-from-faucets-sink-surfaces-and-patients
#18
Cindy Lalancette, Dominique Charron, Céline Laferrière, Patrick Dolcé, Eric Déziel, Michèle Prévost, Emilie Bédard
Identifying environmental sources of Pseudomonas aeruginosa (Pa) related to hospital-acquired infections represents a key challenge for public health. Biofilms in water systems offer protection and favorable growth conditions, and are prime reservoirs of microorganisms. A comparative genotyping survey assessing the relationship between Pa strains recovered in hospital sink biofilm and isolated in clinical specimens was conducted. Environmental strains from drain, faucet and sink-surface biofilm were recovered by a culture method after an incubation time ranging from 48 to 240 h...
August 9, 2017: Pathogens
https://www.readbyqxmd.com/read/28791256/in-vivo-host-pathogen-interaction-as-revealed-by-global-proteomic-profiling-of-zebrafish-larvae
#19
Francisco Díaz-Pascual, Javiera Ortíz-Severín, Macarena A Varas, Miguel L Allende, Francisco P Chávez
The outcome of a host-pathogen interaction is determined by the conditions of the host, the pathogen, and the environment. Although numerous proteomic studies of in vitro-grown microbial pathogens have been performed, in vivo proteomic approaches are still rare. In addition, increasing evidence supports that in vitro studies inadequately reflect in vivo conditions. Choosing the proper host is essential to detect the expression of proteins from the pathogen in vivo. Numerous studies have demonstrated the suitability of zebrafish (Danio rerio) embryos as a model to in vivo studies of Pseudomonas aeruginosa infection...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28790407/employing-high-frequency-alternating-magnetic-fields-for-the-non-invasive-treatment-of-prosthetic-joint-infections
#20
Rajiv Chopra, Sumbul Shaikh, Yonatan Chatzinoff, Imalka Munaweera, Bingbing Cheng, Seth M Daly, Yin Xi, Chenchen Bing, Dennis Burns, David E Greenberg
Treatment of prosthetic joint infection (PJI) usually requires surgical replacement of the infected joint and weeks of antibiotic therapy, due to the formation of biofilm. We introduce a non-invasive method for thermal destruction of biofilm on metallic implants using high-frequency (>100 kHz) alternating magnetic fields (AMF). In vitro investigations demonstrate a >5-log reduction in bacterial counts after 5 minutes of AMF exposure. Confocal and scanning electron microscopy confirm removal of biofilm matrix components within 1 minute of AMF exposure, and combination studies of antibiotics and AMF demonstrate a 5-log increase in the sensitivity of Pseudomonas aeruginosa to ciprofloxacin...
August 8, 2017: Scientific Reports
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