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https://www.readbyqxmd.com/read/29473844/antimicrobial-and-antibiofilm-activity-and-machine-learning-classification-analysis-of-essential-oils-from-different-mediterranean-plants-against-pseudomonas-aeruginosa
#1
Marco Artini, Alexandros Patsilinakos, Rosanna Papa, Mijat Božović, Manuela Sabatino, Stefania Garzoli, Gianluca Vrenna, Marco Tilotta, Federico Pepi, Rino Ragno, Laura Selan
Pseudomonas aeruginosa is a ubiquitous organism and opportunistic pathogen that can cause persistent infections due to its peculiar antibiotic resistance mechanisms and to its ability to adhere and form biofilm. The interest in the development of new approaches for the prevention and treatment of biofilm formation has recently increased. The aim of this study was to seek new non-biocidal agents able to inhibit biofilm formation, in order to counteract virulence rather than bacterial growth and avoid the selection of escape mutants...
February 23, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29472912/the-antimicrobial-activity-of-a-carbon-monoxide-releasing-molecule-ebor-corm-1-is-shaped-by-intraspecific-variation-within-pseudomonas-aeruginosa-populations
#2
Lindsey Flanagan, Rachel R Steen, Karinna Saxby, Mirre Klatter, Benjamin J Aucott, Craig Winstanley, Ian J S Fairlamb, Jason M Lynam, Alison Parkin, Ville-Petri Friman
Carbon monoxide releasing molecules (CORMs) have been suggested as a new synthetic class of antimicrobials to treat bacterial infections. Here we utilized a novel EBOR-CORM-1 ([NEt4 ][MnBr2 (CO)4 ]) capable of water-triggered CO-release, and tested its efficacy against a collection of clinical Pseudomonas aeruginosa strains that differ in infection-related virulence traits. We found that while EBOR-CORM-1 was effective in clearing planktonic and biofilm cells of P. aeruginosa strain PAO1 in a concentration dependent manner, this effect was less clear and varied considerably between different P...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29472905/1-2-4-dichlorophenethyl-amino-3-phenoxypropan-2-ol-kills-pseudomonas-aeruginosa-through-extensive-membrane-damage
#3
Valerie Defraine, Veerle Liebens, Evelien Loos, Toon Swings, Bram Weytjens, Carolina Fierro, Kathleen Marchal, Liam Sharkey, Alex J O'Neill, Romu Corbau, Arnaud Marchand, Patrick Chaltin, Maarten Fauvart, Jan Michiels
The ever increasing multidrug-resistance of clinically important pathogens and the lack of novel antibiotics have resulted in a true antibiotic crisis where many antibiotics are no longer effective. Further complicating the treatment of bacterial infections are antibiotic-tolerant persister cells. Besides being responsible for the recalcitrant nature of chronic infections, persister cells greatly contribute to the observed antibiotic tolerance in biofilms and even facilitate the emergence of antibiotic resistance...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29471219/the-effects-of-extrinsic-factors-on-the-structural-and-mechanical-properties-of-pseudomonas-fluorescens-biofilms-a-combined-study-of-nutrient-concentrations-and-shear-conditions
#4
Ashley Allen, Olivier Habimana, Eoin Casey
The growth of biofilms on surfaces is a complicated process influenced by several environmental factors such as nutrient availability and fluid shear. In this study, combinations of growth conditions were selected for the study of Pseudomonas fluorescens biofilms including as cultivation time (24- or 48 h), nutrient levels (1:1 or 1:10 King B medium), and shear conditions (75 RPM shaking, 0.4 mL min-1 or 0.7 mL min-1 ). The use of Confocal Laser Scanning Microscopy (CLSM) determined biofilm structure, while liquid-phase Atomic Force Microscopy (AFM) techniques resolved the mechanical properties of biofilms...
February 15, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29470394/a-novel-early-warning-system-based-on-a-sediment-microbial-fuel-cell-for-in-situ-and-real-time-hexavalent-chromium-detection-in-industrial-wastewater
#5
Shuai Zhao, Pu Liu, Yongyan Niu, Zhengjun Chen, Aman Khan, Pengyun Zhang, Xiangkai Li
Hexavalent chromium (Cr(VI)) is a well-known toxic heavy metal in industrial wastewater, but in situ and real time monitoring cannot be achieved by current methods used during industrial wastewater treatment processes. In this study, a Sediment Microbial Fuel Cell (SMFC) was used as a biosensor for in situ real-time monitoring of Cr(VI), which was the organic substrate is oxidized in the anode and Cr(VI) is reduced at the cathode simultaneously. The pH 6.4 and temperature 25 °C were optimal conditions for the operation...
February 22, 2018: Sensors
https://www.readbyqxmd.com/read/29470048/designing-porphyrinic-covalent-organic-frameworks-for-the-photodynamic-inactivation-of-bacteria
#6
Jan Hynek, Jaroslav Zelenka, Jiri Rathousky, Pavel Kubát, Tomáš Ruml, Jan Demel, Kamil Lang
Microbial colonization of biomedical devices is a recognized complication contributing to healthcare-associated infections. One of the possible approaches to prevent surfaces from the biofilm formation is antimicrobial photodynamic inactivation, based on the cytotoxic effect of singlet oxygen, O2 (1 Δg ), a short-lived, highly oxidative species, produced by energy transfer between excited photosensitizers and molecular oxygen. We synthesized porphyrin-based covalent organic frameworks (COFs) by the Schiff-base chemistry...
February 22, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29469562/surface-topography-hinders-bacterial-surface-motility
#7
Yow-Ren Chang, Eric R Weeks, William A Ducker
We demonstrate that the surface motility of the bacterium, Pseudomonas aeruginosa, is hindered by a crystalline hemispherical topography with wavelength in the range 2-8 µm. The motility was determined by analysis of time-lapse microscopy images of cells in flowing growth medium maintained at 37°C. The net displacement of bacteria over 5 minutes is much lower on surfaces containing 2-8 µm hemispheres than on flat topography, but displacement on the 1 µm hemispheres is not lower. That is, there is a threshold between 1 µm and 2 µm for response to the topography...
February 22, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29468490/negative-pressure-wound-therapy-reduces-the-motility-of-pseudomonas-aeruginosa-and-enhances-wound-healing-in-a-rabbit-ear-biofilm-infection-model
#8
Wang Guoqi, Li Zhirui, Wang Song, Li Tongtong, Zhang Lihai, Zhang Licheng, Tang Peifu
Pseudomonas aeruginosa motility, virulence factors and biofilms are known to be detrimental to wound healing. The efficacy of negative pressure wound therapy (NPWT) against P. aeruginosa has been little studied, either in vitro or in vivo. The present study evaluated the effect of negative pressure (NP) on P. aeruginosa motility in vitro, and the effect of NPWT on virulence factors and biofilms in vivo. P. aeruginosa motility was quantified under different levels of NP (atmospheric pressure, - 75, - 125, - 200 mmHg) using an in vitro model...
February 21, 2018: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29464939/genome-analysis-of-two-novel-pseudomonas-strains-exhibiting-differential-hypersensitivity-reactions-on-tobacco-seedlings-reveals-differences-in-nonflagellar-t3ss-organization-and-predicted-effector-proteins
#9
Caetanie F Tchagang, Renlin Xu, Cyr Lézin Doumbou, James T Tambong
Multilocus sequence analysis (MLSA) of two new biological control strains (S1E40 and S3E12) of Pseudomonas was performed to assess their taxonomic position relative to close lineages, and comparative genomics employed to investigate whether these strains differ in key genetic features involved in hypersensitivity responses (HRs). Strain S3E12, at high concentration, incites HRs on tobacco and corn plantlets while S1E40 does not. Phylogenies based on individual genes and 16S rRNA-gyrB-rpoB-rpoD concatenated sequence data show strains S1E40 and S3E12 clustering in distinct groups...
February 21, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/29463606/functional-analyses-of-the-rsmy-and-rsmz-small-non-coding-regulatory-rnas-in-pseudomonas-aeruginosa
#10
Kayley H Janssen, Manisha R Diaz, Matthew Golden, Justin W Graham, Wes Sanders, Matthew C Wolfgang, Timothy L Yahr
Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen with distinct acute and chronic virulence phenotypes. Whereas acute virulence is typically associated with expression of a type III secretion system (T3SS), chronic virulence is characterized by biofilm formation. Many of the phenotypes associated with acute and chronic virulence are inversely regulated by RsmA and RsmF. RsmA and RsmF are both members of the CsrA family of RNA-binding proteins and regulate protein synthesis at the post-transcriptional level...
February 20, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29463605/phenazines-regulate-nap-dependent-denitrification-in-pseudomonas-aeruginosa-biofilms
#11
Yu-Cheng Lin, Matthew D Sekedat, William Cole Cornell, Gustavo M Silva, Chinweike Okegbe, Alexa Price-Whelan, Christine Vogel, Lars E P Dietrich
Microbes in biofilms face the challenge of substrate limitation. In particular, cells in Pseudomonas aeruginosa biofilms growing in the laboratory or during host colonization often become limited for oxygen. Previously we found that phenazines, antibiotics produced by P. aeruginosa , balance the intracellular redox state for cells in biofilms. Here, we show that genes involved in denitrification are induced in phenazine-null (Δ phz ) mutant biofilms grown under an aerobic atmosphere, even in the absence of nitrate...
February 20, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29463543/mesoscopic-energy-minimization-drives-pseudomonas-aeruginosa-biofilm-morphologies-and-consequent-stratification-of-antibiotic-activity-based-on-cell-metabolism
#12
M V Sheraton, K H Yam, C H Tan, H S Oh, E Mancini, L Yang, S A Rice, P M A Sloot
Segregation of bacteria based on their metabolic activity in biofilms plays an important role in the development of antibiotic drug resistance. Mushroom-shaped biofilm structures, which are reported for many bacteria, exhibit topographically varying levels of multiple drug resistance from the cap of the mushroom to its stalk. Understanding the dynamics behind the formation of such structures can aid in design of drug delivery systems, antibiotics, or physical systems for removal of biofilms. We explore the development of metabolically heterogenous Pseudomonas aeruginosa biofilms using numerical models and laboratory knock-out experiments on wild-type and chemotaxis deficient mutants...
February 20, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29463534/alginate-oligosaccharide-induced-modification-of-the-lasi-lasr-and-rhli-rhlr-quorum-sensing-systems-in-pseudomonas-aeruginosa
#13
Alison A Jack, Saira Khan, Lydia C Powell, Manon F Pritchard, Konrad Beck, Hina Sadh, Lucy Sutton, Alessandra Cavaliere, Hannah Florance, Philip D Rye, David W Thomas, Katja E Hill
Pseudomonas aeruginosa plays a major role in many chronic infections. Its ability to readily form biofilms contributes to its success as an opportunistic pathogen and its resistance/tolerance to antimicrobial/antibiotic therapy. A low molecular weight alginate oligomer (OligoG CF-5/20), derived from marine algae, has previously been shown to impair motility in P. aeruginosa biofilms and disrupt pseudomonal biofilm assembly. As these bacterial phenotypes are regulated by quorum sensing (QS), we hypothesized that OligoG CF-5/20 may induce alterations in QS signalling in P...
February 20, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29462817/the-interaction-of-n-acetylcysteine-and-serum-transferrin-promotes-bacterial-biofilm-formation
#14
Supeng Yin, Bei Jiang, Guangtao Huang, Yulong Zhang, Bo You, Yu Chen, Yali Gong, Jing Chen, Zhiqiang Yuan, Yan Zhao, Ming Li, Fuquan Hu, Zichen Yang, Yizhi Peng
BACKGROUND/AIMS: N-acetylcysteine (NAC) is a novel and promising agent with activity against bacterial biofilms. Human serum also inhibits biofilm formation by some bacteria. We tested whether the combination of NAC and human serum offers greater anti-biofilm activity than either agent alone. METHODS: Microtiter plate assays and confocal laser scanning microscopy were used to evaluate bacterial biofilm formation in the presence of NAC and human serum. qPCR was used to examine expression of selected biofilm-associated genes...
February 15, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29454827/development-of-thermostable-amylase-enzyme-from-bacillus-cereus-for-potential-antibiofilm-activity
#15
Ramalingam Vaikundamoorthy, Rajaram Rajendran, Ananth Selvaraju, Kaviyarasan Moorthy, Santhanam Perumal
The marine bacterial strain Bacillus cereus was used to produce amylase enzyme and has excellent alkali-stable and thermostable enzymatic activity. The combined effects of pH, temperature and incubation time on amylase activity were studied using response surface methodology. The amylase enzyme activity was also determined in the presence of various metal ions, chelating agents, detergents and the results showed that the maximum enzyme activity was observed in the presence of calcium chloride (96.1%), EDTA (63...
February 12, 2018: Bioorganic Chemistry
https://www.readbyqxmd.com/read/29451920/an-upstream-sequence-modulates-phenazine-production-at-the-level-of-transcription-and-translation-in-the-biological-control-strain-pseudomonas-chlororaphis-30-84
#16
Jun Myoung Yu, Dongping Wang, Tessa R Ries, Leland S Pierson, Elizabeth A Pierson
Phenazines are bacterial secondary metabolites and play important roles in the antagonistic activity of the biological control strain P. chlororaphis 30-84 against take-all disease of wheat. The expression of the P. chlororaphis 30-84 phenazine biosynthetic operon (phzXYFABCD) is dependent on the PhzR/PhzI quorum sensing system located immediately upstream of the biosynthetic operon as well as other regulatory systems including Gac/Rsm. Bioinformatic analysis of the sequence between the divergently oriented phzR and phzX promoters identified features within the 5'-untranslated region (5'-UTR) of phzX that are conserved only among 2OHPCA producing Pseudomonas...
2018: PloS One
https://www.readbyqxmd.com/read/29449837/cyclic-di-gmp-and-oprf-are-involved-in-the-response-of-pseudomonas-aeruginosa-to-substrate-material-stiffness-during-attachment-on-polydimethylsiloxane-pdms
#17
Fangchao Song, Hao Wang, Karin Sauer, Dacheng Ren
Recently, we reported that the stiffness of poly(dimethylsiloxane) (PDMS) affects the attachment of Pseudomonas aeruginosa , and the morphology and antibiotic susceptibility of attached cells. To further understand how P. aeruginosa responses to material stiffness during attachment, the wild-type P. aeruginosa PAO1 and several isogenic mutants were characterized for their attachment on soft and stiff PDMS. Compared to the wild-type strain, mutation of the oprF gene abolished the differences in attachment, growth, and size of attached cells between soft and stiff PDMS surfaces...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29449129/sub-minimum-inhibitory-concentrations-of-ceftazidime-inhibit-pseudomonas-aeruginosa-biofilm-formation
#18
Satoshi Otani, Kazufumi Hiramatsu, Kazuhiko Hashinaga, Kosaku Komiya, Kenji Umeki, Kenji Kishi, Jun-Ichi Kadota
Pseudomonas aeruginosa exhibits the biofilm mode of growth and causes chronic as well as acute infections in humans. Several reports have shown that the treatments with sub-minimum inhibitory concentrations (sub-MICs) of antimicrobial agents influence biofilm formation by P. aeruginosa. The antibiotic ceftazidime (CAZ) is used to treat P. aeruginosa infections, but few studies have examined the effects of β-lactams on biofilm formation by P. aeruginosa. In this study, we investigated the role of sub-MICs of CAZ in the formation of P...
February 12, 2018: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/29449125/enhancing-the-anti-biofilm-activity-of-5-aryl-2-aminoimidazoles-through-nature-inspired-dimerisation
#19
Tran Thi Thu Trang, Lise Dieltjens, Geert Hooyberghs, Kai Waldrant, Denis S Ermolat'ev, Erik V Van der Eycken, Hans P L Steenackers
The increased tolerance of biofilms against disinfectants and antibiotics has stimulated research into new methods of biofilm prevention and eradication. In our previous work, we have identified the 5-aryl-2-aminoimidazole core as a scaffold that demonstrates preventive activity against biofilm formation of a broad range of bacterial and fungal species. Inspired by the dimeric nature of natural 2-aminoimidazoles of the oroidin family, we investigated the potential of dimers of our decorated 5-aryl-2-aminoimidazoles as biofilm inhibitors...
January 31, 2018: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29446642/revealing-the-dual-role-of-gallic-acid-in-modulating-ampicillin-sensitivity-of-pseudomonas-aeruginosa-biofilms
#20
Rekha Yamini Kosuru, Md Aashique, Aisha Fathima, Amrita Roy, Soumen Bera
AIM: To understand the effects of gallic acid (GA) on ampicillin (Amp) sensitive or resistant strain of Pseudomonas sp. and also in modulating the corresponding biofilms. METHODOLOGY: The cell viability was determined by broth dilution, dry weight and CFU assays. Biofilm formation was measured by crystal violet assay while oxygen consumption rate was measured to verify the metabolic status of the cells. The membrane damage and drug efflux/accumulation were studied by fluorimetric assays...
February 15, 2018: Future Microbiology
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