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https://www.readbyqxmd.com/read/29042302/attenuation-of-pseudomonas-aeruginosa-quorum-sensing-virulence-and-biofilm-formation-by-extracts-of-andrographis-paniculata
#1
Malabika Banerjee, Soumitra Moulick, Kunal Kumar Bhattacharya, Debaprasad Parai, Subrata Chattopadhyay, Samir Kumar Mukherjee
Quorum-sensing (QS) is known to play an essential role in regulation of virulence factors and toxins during Pseudomonas aeruginosa infection which may frequently cause antibiotic resistance and hostile outcomes of inflammatory injury. Therefore, it is an urgent need to search for a novel agent with low risk of resistance development that can target QS and inflammatory damage prevention as well. Andrographis paniculata, a herbaceous plant under the family Acanthaceae, native to Asian countries and also cultivated in Scandinavia and some parts of Europe, has a strong traditional usage with its known antibacterial, anti-inflammatory, antipyretic, antiviral and antioxidant properties...
October 14, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29040295/hydrophobic-residues-are-critical-for-the-helix-forming-hemolytic-and-bactericidal-activities-of-amphipathic-antimicrobial-peptide-tp4
#2
Ting-Wei Chang, Shu-Yi Wei, Shih-Han Wang, Hung-Mu Wei, Yu-June Wang, Chiu-Feng Wang, Chinpan Chen, You-Di Liao
Antimicrobial peptides are important components of the host innate defense mechanism against invading pathogens, especially for drug-resistant bacteria. In addition to bactericidal activity, the 25 residue peptide TP4 isolated from Nile tilapia also stimulates cell proliferation and regulates the innate immune system in mice. In this report, TP4 hyperpolarized and depolarized the membrane potential of Pseudomonas aeruginosa at sub-lethal and lethal concentrations. It also inhibited and eradicated biofilm formation...
2017: PloS One
https://www.readbyqxmd.com/read/29039290/simulated-use-polytetrafluorethylene-biofilm-model-repeated-rounds-of-complete-reprocessing-lead-to-accumulation-of-organic-debris-and-viable-bacteria
#3
Michelle J Alfa, Harminder Singh, Zoann Nugent, Donald Duerksen, Gale Schultz, Carol Reidy, Pat DeGagne, Nancy Olson
OBJECTIVE Biofilm has been implicated in bacterial persistence and survival after endoscope reprocessing. In this study, we assessed the impact of different methods of reprocessing on organic residues and viable bacteria after repeated rounds of biofilm formation when each was followed by full reprocessing. METHODS ATS-2015, an artificial test soil containing 5-8 Log10 colony-forming units (CFU) of Enterococcus faecalis and Pseudomonas aeruginosa, was used to form biofilm in polytetrafluroethylene channels overnight on 5 successive days...
October 17, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/29039211/synergistic-effects-of-heat-and-antibiotics-on-pseudomonas-aeruginosa-biofilms
#4
Erica B Ricker, Eric Nuxoll
Upon formation of a biofilm, bacteria undergo several changes that prevent eradication with antimicrobials alone. Due to this resistance, the standard of care for infected medical implants is explantation of the infected implant and surrounding tissue, followed by eventual reimplantation of a replacement device. Recent studies have demonstrated the efficacy of heat shock for biofilm eradication. To minimize the heat required for in situ biofilm eradication, this study investigated the hypothesis that antibiotics, while ineffective by themselves, may substantially increase heat shock efficacy...
October 17, 2017: Biofouling
https://www.readbyqxmd.com/read/29038255/studies-of-pseudomonas-aeruginosa-mutants-indicate-pyoverdine-as-the-central-factor-in-inhibition-of-aspergillus-fumigatus-biofilm
#5
Gabriele Sass, Hasan Nazik, John Penner, Hemi Shah, Shajia Rahman Ansari, Karl V Clemons, Marie-Christine Groleau, Anna-Maria Dietl, Paolo Visca, Hubertus Haas, Eric Déziel, David A Stevens
Pseudomonas aeruginosa (Pa) and Aspergillus fumigatus (Af) are common opportunistic bacterial and fungal pathogens, respectively. They often co-exist in airways of immunocompromised patients and individuals with cystic fibrosis, where they form biofilms and cause acute and chronic illnesses. Hence the interactions between them have long been of interest, and it is known that Pa can inhibit Af in vitro We have approached the definition of the inhibitory Pa molecules by studying 24 Pa mutants, with various virulence genes deleted, for their ability to inhibit Af biofilms...
October 16, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/29038052/serine-threonine-protein-kinase-ppka-contributes-to-the-adaptation-and-virulence-in-pseudomonas-aeruginosa
#6
Jianyi Pan, Zhenzhong Zha, Pengfei Zhang, Ran Chen, Chen Ye, Ting Ye
Pseudomonas aeruginosa is a leading cause of nosocomial infections worldwide and has become a serious public health threat, which is attributed to a large extent to its extraordinary environmental adaptability and diverse virulence factors that result in infection and progression of pathogenesis. The eukaryote-type serine/threonine (Ser/Thr) protein kinases, known for playing major regulatory roles in eukaryotes, have been demonstrated to play a central role in regulating various bacterial cellular processes via catalyzing protein phosphorylation...
October 13, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29034850/pseudomonas-aeruginosa-variants-obtained-from-veterinary-clinical-samples-reveal-a-role-for-cyclic-di-gmp-in-biofilm-formation-and-colony-morphology
#7
Maria T Brock, Galya C Fedderly, Grace I Borlee, Michael M Russell, Liliana K Filipowska, Doreene R Hyatt, Ryan A Ferris, Bradley R Borlee
Overuse of antibiotics is contributing to an emerging antimicrobial resistance crisis. To better understand how bacteria adapt tolerance and resist antibiotic treatment, Pseudomonas aeruginosa isolates obtained from infection sites sampled from companion animals were collected and evaluated for phenotypic differences. Selected pairs of clonal isolates were obtained from individual infection samples and were assessed for antibiotic susceptibility, cyclic di-GMP levels, biofilm production, motility and genetic-relatedness...
October 16, 2017: Microbiology
https://www.readbyqxmd.com/read/29033313/developing-a-model-for-cystic-fibrosis-sociomicrobiology-based-on-antibiotic-and-environmental-stress
#8
Susana Patrícia Lopes, Nuno Filipe Azevedo, Maria Olívia Pereira
Cystic fibrosis (CF) infections are invariably biofilm-mediated and polymicrobial, being safe to assume that a myriad of factors affects the sociomicrobiology within the CF infection site and modulate the CF community dynamics, by shaping their social activities, overall functions, virulence, ultimately affecting disease outcome. This work aimed to assess changes in the dynamics (particularly on the microbial composition) of dual-/three-species biofilms involving CF-classical (Pseudomonas aeruginosa) and unusual species (Inquilinus limosus and Dolosigranulum pigrum), according to variable oxygen conditions and antibiotic exposure...
September 28, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29033275/mechanisms-of-humoral-immune-response-against-pseudomonas-aeruginosa-biofilm-infection-in-cystic-fibrosis
#9
REVIEW
Renan Marrichi Mauch, Peter Østrup Jensen, Claus Moser, Carlos Emilio Levy, Niels Høiby
P. aeruginosa chronic lung infection is the major cause of morbidity and mortality in patients with cystic fibrosis (CF), and is characterized by a biofilm mode of growth, increased levels of specific IgG antibodies and immune complex formation. However, despite being designed to combat this infection, such elevated humoral response is not associated with clinical improvement, pointing to a lack of anti-pseudomonas effectiveness. The mode of action of specific antibodies, as well as their structural features, and even the background involving B-cell production, stimulation and differentiation into antibody-producing cells in the CF airways are poorly understood...
October 12, 2017: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
https://www.readbyqxmd.com/read/29032178/igg-avidity-to-pseudomonas-aeruginosa-over-the-course-of-chronic-lung-biofilm-infection-in-cystic-fibrosis
#10
Renan Marrichi Mauch, Lena Lingren Nørregaard, Oana Ciofu, Carlos Emilio Levy, Niels Høiby
BACKGROUND AND OBJECTIVES: The mechanisms leading to low effectiveness of the humoral immune response against P. aeruginosa in cystic fibrosis (CF) are poorly understood. The aim of the present study was to assess the avidity maturation of specific antipseudomonal IgG before and during the development of chronic lung infection in a cohort of Danish CF patients. METHODS: Avidity maturation was assessed against a pooled P. aeruginosa antigen (St-Ag) and against P...
October 11, 2017: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
https://www.readbyqxmd.com/read/29031224/direct-one-pot-synthesis-of-cinnamaldehyde-immobilized-on-gold-nanoparticles-and-their-antibiofilm-properties
#11
Mohankandhasamy Ramasamy, Jin-Hyung Lee, Jintae Lee
The objective of the present study was to develop a one-pot strategy to synthesis gold nanoparticle complexes using cinnamaldehyde, a potent antibiofilm agent which in its free form, exhibits high volatility and unstable nature. Hence, we developed cinnamaldehyde gold nanoparticles (CGNPs) in a single step to overcome the limitations of free cinnamaldehyde. Furthermore, reduction abilities of cinnamaldehyde under different experimental conditions, that is, varying precursor concentrations of cinnamaldehyde and gold, metal salts, pH, temperature, and light sources, were investigated...
October 6, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29030956/spatial-transcriptomes-within-the-pseudomonas-aeruginosa-biofilm-architecture
#12
Yun Heacock-Kang, Zhenxin Sun, Jan Zarzycki-Siek, Ian A McMillan, Michael H Norris, Andrew P Bluhm, Darlene Cabanas, Dawson Fogen, Hung Vo, Stuart P Donachie, Bradley R Borlee, Christopher D Sibley, Shawn Lewenza, Michael J Schurr, Herbert P Schweizer, Tung T Hoang
Bacterial cooperative associations and dynamics in the biofilm microenvironments are of special interest in recent years. Knowledge of localized gene-expression and corresponding bacterial behaviors within the biofilm architecture at a global scale has been limited, due to a lack of robust technology to study limited number of cells in stratified layers of biofilms. With our recent pioneering developments in single bacterial cell transcriptomic analysis technology, we generated herein an unprecedented spatial transcriptome map of the mature in vitro Pseudomonas aeruginosa biofilm model, revealing contemporaneous yet altered bacterial behaviors at different layers within the biofilm architecture (i...
October 14, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/29025325/versatility-of-targeted-antibiotic-loaded-gold-nanoconstructs-for-the-treatment-of-biofilm-associated-bacterial-infections
#13
Daniel G Meeker, Tengjiao Wang, Walter N Harrington, Vladimir P Zharov, Sarah A Johnson, Samir V Jenkins, Stephanie E Oyibo, Christopher M Walker, Weston B Mills, Mark E Shirtliff, Karen E Beenken, Jingyi Chen, Mark S Smeltzer
BACKGROUND: We previously demonstrated that a photoactivatable therapeutic approach employing antibiotic-loaded, antibody-conjugated, polydopamine (PDA)-coated gold nanocages (AuNCs) could be used for the synergistic killing of bacterial cells within a biofilm. The approach was validated with a focus on Staphylococcus aureus using an antibody specific for staphylococcal protein A (Spa) and an antibiotic (daptomycin) active against Gram-positive cocci including methicillin-resistant S...
October 12, 2017: International Journal of Hyperthermia
https://www.readbyqxmd.com/read/29021784/synthetic-peptides-to-target-stringent-response-controlled-virulence-in-a-pseudomonas-aeruginosa-murine-cutaneous-infection-model
#14
Daniel Pletzer, Heidi Wolfmeier, Manjeet Bains, Robert E W Hancock
Microorganisms continuously monitor their surroundings and adaptively respond to environmental cues. One way to cope with various stress-related situations is through the activation of the stringent stress response pathway. In Pseudomonas aeruginosa this pathway is controlled and coordinated by the activity of the RelA and SpoT enzymes that metabolize the small nucleotide secondary messenger molecule (p)ppGpp. Intracellular ppGpp concentrations are crucial in mediating adaptive responses and virulence. Targeting this cellular stress response has recently been the focus of an alternative approach to fight antibiotic resistant bacteria...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29018773/activity-of-bacteriophages-in-removing-biofilms-of-pseudomonas-aeruginosa-isolates-from-chronic-rhinosinusitis-patients
#15
Stephanie A Fong, Amanda Drilling, Sandra Morales, Marjolein E Cornet, Bradford A Woodworth, Wytske J Fokkens, Alkis J Psaltis, Sarah Vreugde, Peter-John Wormald
Introduction:Pseudomonas aeruginosa infections are prevalent amongst chronic rhinosinusitis (CRS) sufferers. Many P. aeruginosa strains form biofilms, leading to treatment failure. Lytic bacteriophages (phages) are viruses that infect, replicate within, and lyse bacteria, causing bacterial death. Aim: To assess the activity of a phage cocktail in eradicating biofilms of ex vivo P.aeruginosa isolates from CRS patients. Methods: P. aeruginosa isolates from CRS patients with and without cystic fibrosis (CF) across three continents were multi-locus sequence typed and tested for antibiotic resistance...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28994493/biofilm-removal-potential-of-neutral-electrolyzed-water-on-pathogen-and-spoilage-bacteria-in-dairy-model-systems
#16
Mehran Moradi, Hossein Tajik
AIMS: This study aimed to investigate the efficacy of neutral electrolyzed water (NEW) on biofilm and planktonic forms of Listeria monocytogenes, Escherichia coli and Pseudomonas aeruginosa in Luria-Bertani (LB) broth, 0.5 and 3% w/w fat pasteurized and UHT milk. ' METHODS AND RESULTS: Biofilm was established on stainless steels in a simulated industrial model biofilm reactor. NEW had a bactericidal effect on planktonic forms of all bacteria at 25 μg mL(-1) concentration...
October 10, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28990487/gaba-a-primary-metabolite-controlled-by-the-gac-rsm-regulatory-pathway-favors-a-planktonic-over-a-biofilm-lifestyle-in-pseudomonas-protegens-cha0
#17
Kasumi Takeuchi
In Pseudomonas protegens CHA0 and other fluorescent pseudomonads, the Gac/Rsm signal transduction pathway is crucial for the expression of secondary metabolism and the biological control of fungi, nematodes, and insects. Based on the findings of a previous metabolomic study, the role of intracellular -aminobutyrate (GABA) as a potential signal in the Gac/Rsm pathway was investigated herein. The function and regulation of a gabDT (c01870-c01880) gene cluster in strain CHA0 were described. The gabT gene encoded -aminobutyrate transaminase (GABAT) and enabled the growth of the bacterium on GABA, whereas the upstream gabD gene (annotated as a gene encoding succinic semialdehyde dehydrogenase) had an unknown function...
October 9, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28989300/study-of-crystal-formation-and-nitric-oxide-no-release-mechanism-from-s-nitroso-n-acetylpenicillamine-snap-doped-carbosil-polymer-composites-for-potential-antimicrobial-applications
#18
Yaqi Wo, Zi Li, Alessandro Colletta, Jianfeng Wu, Chuanwu Xi, Adam J Matzger, Elizabeth J Brisbois, Robert H Bartlett, Mark E Meyerhoff
Stable and long-term nitric oxide (NO) releasing polymeric materials have many potential biomedical applications. Herein, we report the real-time observation of the crystallization process of the NO donor, S-nitroso-N-acetylpenicillamine (SNAP), within a thermoplastic silicone-polycarbonate-urethane biomedical polymer, CarboSil 20 80A. It is demonstrated that the NO release rate from this composite material is directly correlated with the surface area that the CarboSil polymer film is exposed to when in contact with aqueous solution...
July 15, 2017: Compos B Eng
https://www.readbyqxmd.com/read/28983655/mechanistic-understanding-of-phenyllactic-acid-mediated-inhibition-of-quorum-sensing-and-biofilm-development-in-pseudomonas-aeruginosa
#19
Maitrayee Chatterjee, Sharon D'Morris, Vinod Paul, Sruthi Warrier, Anil Kumar Vasudevan, Muralidharan Vanuopadath, Sudarslal Sadasivan Nair, Bindhu Paul-Prasanth, C Gopi Mohan, Raja Biswas
Pseudomonas aeruginosa depends on its quorum sensing (QS) system for its virulence factors' production and biofilm formation. Biofilms of P. aeruginosa on the surface of indwelling catheters are often resistant to antibiotic therapy. Alternative approaches that employ QS inhibitors alone or in combination with antibiotics are being developed to tackle P. aeruginosa infections. Here, we have studied the mechanism of action of 3-Phenyllactic acid (PLA), a QS inhibitory compound produced by Lactobacillus species, against P...
October 5, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28982395/pseudomonas-phage-inhibition-of-candida-albicans
#20
Hasan Nazik, Lydia-Marie Joubert, Patrick R Secor, Johanna M Sweere, Paul L Bollyky, Gabriele Sass, Lynette Cegelski, David A Stevens
Pseudomonas aeruginosa (Pa) and Candida albicans (Ca) are major bacterial and fungal pathogens in immunocompromised hosts, and notably in the airways of cystic fibrosis patients. The bacteriophages of Pa physically alter biofilms, and were recently shown to inhibit the biofilms of Aspergillus fumigatus. To understand the range of this viral-fungal interaction, we studied Pa phages Pf4 and Pf1, and their interactions with Ca biofilm formation and preformed Ca biofilm. Both forms of Ca biofilm development, as well as planktonic Ca growth, were inhibited by either phage...
October 6, 2017: Microbiology
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