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Zehui Qu, Fei Gao, Liwei Li, Yujiao Zhang, Yifeng Jiang, Lingxue Yu, Yanjun Zhou, Hao Zheng, Wu Tong, Guoxin Li, Guangzhi Tong
Significant differences exist between the highly pathogenic (HP) porcine reproductive and respiratory syndrome virus (PRRSV) and its attenuated pathogenic (AP) strain in the ability to infect host cells. The mechanisms by which different virulent strains invade host cells remain relatively unknown. In this study, pulmonary alveolar macrophages (PAMs) were infected with HP-PRRSV (HuN4) and AP-PRRSV (HuN4-F112) for 24 h, then harvested and subjected to label-free quantitative mass spectrometry. A total of 2849 proteins were identified, including 95 that were differentially expressed...
October 20, 2017: Proteomics
Randall S Prather, Kevin D Wells, Kristin M Whitworth, Maureen A Kerrigan, Melissa S Samuel, Alan Mileham, Luca N Popescu, Raymond R R Rowland
After infection of the porcine dam at about 90 days of gestation, porcine reproductive and respiratory syndrome virus (PRRSV) crosses the placenta and begins to infect fetuses. Outcomes of include abortion, fetal death and respiratory disease in newborn piglets. CD163 is the receptor for the virus. In this study, CD163-positive fetuses, recovered between 109 days of gestation or 20 days after maternal infection, were completely protected from PRRSV in dams possessing a complete knockout of the CD163 receptor...
October 17, 2017: Scientific Reports
Cindy Wenke, Janina Pospiech, Tobias Reutter, Uwe Truyen, Stephanie Speck
Air filtration has been shown to be efficient in reducing pathogen burden in circulating air. We determined at laboratory scale the retention efficiency of different air filter types either composed of a prefilter (EU class G4) and a secondary fiberglass filter (EU class F9) or consisting of a filter mat (EU class M6 and F8-9). Four filter prototypes were tested for their capability to remove aerosol containing equine arteritis virus (EAV), porcine reproductive and respiratory syndrome virus (PRRSV), bovine enterovirus 1 (BEV), Actinobacillus pleuropneumoniae (APP), and Staphylococcus (S...
2017: PloS One
Jeoung Hwa Shin, Won Hyun Ji, Chanhee Chae, Shela Gorinstein, Yun Gyong Ahn
This study deals with the levels of toxic polychlorinated dibenzo-p-dioxin and furan congeners (PCDD/Fs) in the livers of piglets affected by infectious diseases using isotope dilution high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS). Seventeen toxic congeners in the liver samples infected with bacterial and viral diseases were compared. For porcine reproductive and respiratory syndrome virus (PRRSV) samples, the North American- and European-type PRRS diseases were observed. This study shows that there are significantly different levels of PCDD/Fs, present, which vary according to the types of diseases as evidenced by our analysis of the piglet liver samples...
August 2017: International Journal of Experimental Pathology
Taofeng Du, Yunpeng Shi, Shuqi Xiao, Na Li, Qin Zhao, Angke Zhang, Yuchen Nan, Yang Mu, Yani Sun, Chunyan Wu, Hongtao Zhang, En-Min Zhou
BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV) could lead to pandemic diseases and huge financial losses to the swine industry worldwide. Curcumin, a natural compound, has been reported to serve as an entry inhibitor of hepatitis C virus, chikungunya virus and vesicular stomatitis virus. In this study, we investigated the potential effect of curcumin on early stages of PRRSV infection. RESULTS: Curcumin inhibited infection of Marc-145 cells and porcine alveolar macrophages (PAMs) by four different genotype 2 PRRSV strains, but had no effect on the levels of major PRRSV receptor proteins on Marc-145 cells and PAMs or on PRRSV binding to Marc-145 cells...
October 10, 2017: BMC Veterinary Research
Tamás Süli, Máté Halas, Zsófia Benyeda, Réka Boda, Sándor Belák, Marta Martínez-Avilés, Eduardo Fernández-Carrión, José Manuel Sánchez-Vizcaíno
Highly contagious and emerging diseases cause significant losses in the pig producing industry worldwide. Rapid and exact acquisition of real-time data, like body temperature and animal movement from the production facilities would enable early disease detection and facilitate adequate response. In this study, carried out within the European Union research project RAPIDIA FIELD, we tested an online monitoring system on pigs experimentally infected with the East European subtype 3 Porcine Reproductive & Respiratory Syndrome Virus (PRRSV) strain Lena...
September 28, 2017: Research in Veterinary Science
Yonlayong Woonwong, Roongtham Kedkovid, Jirapat Arunorat, Chaitawat Sirisereewan, Teerawut Nedumpun, Korakrit Poonsuk, Yaowalak Panyasing, Pariwat Poolperm, Alongkot Boonsoongnern, Roongroje Thanawongnuwech
An effective gilt acclimatization program is one of the most important management strategies for controlling porcine reproductive and respiratory syndrome virus (PRRSV) infection. Recently, oral fluid samples have been used as alternative diagnostic samples for various swine diseases. This study utilized oral fluids for PRRSV monitoring during the gilt acclimatization period in PRRSV endemic farms. The study was performed in two selected commercial breeding herds (farm A and farm B). PRRSV RNA and PRRSV-specific antibodies were monitored using oral fluid and serum samples...
October 5, 2017: Tropical Animal Health and Production
Xiang Zhou, Jennifer J Michal, Zhihua Jiang, Bang Liu
Porcine respiratory and reproductive syndrome (PRRS), caused by PRRS virus (PRRSV), is one of the most serious infectious diseases in the swine industry worldwide. Indigenous Chinese Tongcheng (TC) pigs reportedly show strong resistance to PRRSV infection. The miRNA expression profiles of porcine alveolar macrophages (PAMs) of control TC pigs and those infected with PRRSV in vivo were analyzed by high-throughput sequencing to explore changes induced by infection. A total of 182 known miRNAs including 101 miRNA-5p and 81 miRNA-3p were identified with 23 up-regulated differentially expressed miRNAs (DEmiRNAs) and 25 down-regulated DEmiRNAs...
October 2, 2017: Journal of Applied Genetics
Xi-Wen Chen, Lian Li, Miao Yin, Qian Wang, Wen-Tao Luo, Ying Ma, Zhong-Hui Pu, Jie-Long Zhou
To monitor the genetic variation of PRRSV, the ORF5 gene of the PRRSV-SN strain found in Suining City, Sichuan Province, was cloned and sequenced. The results showed that the PRRSV-SN strain was a highly pathogenic PRRSV (HP-PRRSV) variant strain with the North American (NA) genotype. Homology analysis showed that the ORF5 gene of the PRRSV-SN isolate shared 89.4% (86.5%) nucleotide (amino acid) sequence similarity with the North American strain VR-2332, 98.8% (96%) similarity with JXA1, and 63.8% (57.7%) similarity with the European type representative strain Lelystad virus...
September 29, 2017: Microbial Pathogenesis
Guangping Huang, Xianyong Liu, Xiaoli Tang, Li Du, Wenhai Feng, Xiaoxiang Hu, Liangquan Zhu, Qiuyan Li, Xun Suo
T cell-mediated immunity plays a prominent role in combating pathogens infection. Both the engagement of the T cell receptor with the peptide-bound major histocompatibility complex and a costimulatory signal are needed for the complete activation of the T cell. To determine whether host immune responses to vaccination could be improved by enhancing CD28-mediated costimulation and verify whether the boosted immune responses could protect the host against viral challenge, we produced a transgenic pig line expressing an extra copy of the CD28 gene controlled by its own promoter at the Rosa26 locus...
2017: Frontiers in Immunology
Christiane Weissenbacher-Lang, Nora Nedorost, Christian Knecht, Isabel Hennig-Pauka, Mathias Huber, Thomas Voglmayr, Herbert Weissenböck
Pneumocystis carinii f. sp. suis (PCS) nucleic acid and antibody profiles on two Austrian-farrow-to-finish farms were investigated. Furthermore, associations with other respiratory pathogens were evaluated. Respiratory specimen and sera from pigs of five age classes between the 1st week and the 3rd month of life as well as samples from sows were analyzed. On Farm A, PCS infection occurred early in life. The suckling piglets were already infected in the 1st week of life and the pigs remained positive until the 3rd month of life...
2017: PloS One
Fanfan Zhang, Yu Ye, Deping Song, Nannan Guo, Qi Peng, Anqi Li, Xingrong Zhou, Yanjun Chen, Min Zhang, Dongyan Huang, Yuxin Tang
BACKGROUND: Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has spread to many countries around the world, leading to significant economic losses in the pork industry. Therefore, a rapid and sensitive method for detection of PDCoV in clinical samples is urgently needed. RESULTS: In this study, we developed a single-tube one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay specific for nucleocapsid gene to diagnose and monitor PDCoV infections...
September 21, 2017: Biological Research
Yang Zong, Xiaoyin Zong, Wenlong Xia, Zhi Wu, Guangya Li, Yangyang Li, Xinyu Zhang, Xiaoli Xia, Huaichang Sun
Environmental surveillance of porcine reproductive and respiratory syndrome virus (PRRSV) represents a key issue in control of the disease. CD151 has recently been recognized as one of several receptors for PRRSV. We describe here a novel method for concentration of PRRSV from the environmental samples by CD151-binding capture. After fusion to self-aggregating peptide ELK16, the large extracellular loop (LEL) of porcine CD151 and its two segments (namely N63 and C63) were expressed in E. coli as protein aggregates...
September 19, 2017: Applied Microbiology and Biotechnology
Longtao Wang, Shouping Hu, Qiang Liu, Yiru Li, Lu Xu, Zhuo Zhang, Xuehui Cai, Xijun He
Macrophage polarization is a process by which macrophages acquire a distinct phenotypic and functional profile in response to microenvironmental signals. The classically and alternatively activated (M1 and M2, respectively) macrophage phenotypes are defined by the specific molecular characteristics induced in response to prototypic pro- and anti-inflammatory cues. In this study, we used LPS/IFN-γ and IL-4 to stimulate porcine alveolar macrophages (PAMs) in vitro and investigated the expression changes of several novel markers during macrophage polarization...
September 17, 2017: Journal of Veterinary Medical Science
X X Wang, F X Wang, Z G Li, Y J Wen, X Wang, N Song, H Wu
An accurate ELISA method to differentiate pigs infected with wild-type porcine reproductive and respiratory syndrome (PRRSV) strains from vaccinated ones would help to monitor PRRSV vaccination compliance. The recombinant protein GST-d120aa derived from the continuous deletion of 120 amino acids in the non-structural protein 2 region of the modified-live vaccine strain TJM-F92 was used to develop an indirect enzyme-linked immunosorbent assay (d120-ELISA) for differentiating serum antibodies against TJM-F92 from other PRRSV strains...
September 12, 2017: Journal of Virological Methods
Fanfan Zhang, Deping Song, Yu Ye, Nannan Guo, Min Zhang, Hao Li, Dan Lei, Wang Gong, Suxian Luo, Dongyan Huang, Yuxin Tang
We sequenced the complete genome of porcine reproductive and respiratory syndrome virus (PRRSV) strain JX/CH/2016. Phylogenetic analysis based on the sequences of the open reading frame 5 (ORF5) gene revealed that this strain belongs to subgenotype IV. This is the first report of the complete genome sequence of PRRSV-IV.
September 14, 2017: Genome Announcements
Yuming Li, Zhuanchang Wu, Ke Liu, Pengfei Qi, Jinpeng Xu, Jianchao Wei, Beibei Li, Donghua Shao, Yuanyuan Shi, Yafeng Qiu, Zhiyong Ma
Porcine reproductive and respiratory syndrome virus (PRRSV) has an important impact on the pig industry. Doxycycline (Dox) is a second-generation tetracycline widely used for treating bacterial infections. We evaluated the antiviral effect of Dox against PRRSV infection in Marc-145 cells that were susceptible to PRRSV infection. Dox significantly reduced the PRRSV-induced cytopathic effect and effectively restrained PRRSV replication in a dose-dependent manner. The 50% effective concentration of Dox was approximately 0...
September 15, 2017: FEMS Microbiology Letters
Yuming Li, Zhuanchang Wu, Ke Liu, Pengfei Qi, Jinpeng Xu, Jianchao Wei, Beibei Li, Donghua Shao, Yuanyuan Shi, Yafeng Qiu, Zhiyong Ma
Porcine reproductive and respiratory syndrome virus (PRRSV) causes porcine reproductive and respiratory syndrome, which is characterized by reproductive failure and respiratory disorders. The secretome of PRRSV-infected porcine alveolar macrophages (PAMs), which are the primary target cells of PRRSV, was analyzed by label-free quantitative proteomics to gain a profile of proteins secreted during PRRSV infection. A total of 95 secreted proteins with differentially expressed levels between PRRSV- and mock-infected PAMs was screened...
September 12, 2017: Proteomics
Yuchen Nan, Chunyan Wu, Guoqian Gu, Weiyao Sun, Yan-Jin Zhang, En-Min Zhou
Porcine reproductive and respiratory syndrome virus (PRRSV), one of the most economically significant pathogens worldwide, has caused numerous outbreaks during the past 30 years. PRRSV infection causes reproductive failure in sows and respiratory disease in growing and finishing pigs, leading to huge economic losses for the swine industry. This impact has become even more significant with the recent emergence of highly pathogenic PRRSV strains from China, further exacerbating global food security. Since new PRRSV variants are constantly emerging from outbreaks, current strategies for controlling PRRSV have been largely inadequate, even though our understanding of PRRSV virology, evolution and host immune response has been rapidly expanding...
2017: Frontiers in Microbiology
Rebecca A Ober, James B Thissen, Crystal J Jaing, Ada G Cino-Ozuna, Raymond R R Rowland, Megan C Niederwerder
Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) are two of the most important pathogens affecting the swine industry worldwide. Co-infections are common on a global scale, resulting in pork production losses through reducing weight gain and causing respiratory disease in growing pigs. Our initial work demonstrated that the fecal microbiome was associated with clinical outcome of pigs 70days post-infection (dpi) with PRRSV and PCV2. However, it remained uncertain if microbiome characteristics could predispose response to viral infection...
September 2017: Veterinary Microbiology
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