keyword
MENU ▼
Read by QxMD icon Read
search

Tandem

keyword
https://www.readbyqxmd.com/read/28214997/the-human-rit2-core-promoter-short-tandem-repeat-predominant-allele-is-species-specific-in-length-a-selective-advantage-for-human-evolution
#1
Babak Emamalizadeh, Abofazl Movafagh, Hossein Darvish, Somayeh Kazeminasab, Monavvar Andarva, Pegah Namdar-Aligoodarzi, Mina Ohadi
Evolutionary analyses of the critical core promoter interval support a selective advantage for expanding the length of certain short tandem repeats (STRs) in humans. We recently reported genome-wide data on human core promoter STRs that are "exceptionally long" (≥6-repeats). Near the top of the list, the neuron-specific gene, RIT2, contains one of the longest GA-STRs at 11-repeats. In the present study, we analyzed the evolutionary implications of this STR across species. We also studied this STR in a sample of 2,143 Iranian human subjects that encompassed a number of neuropsychiatric disorders and controls...
February 18, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/28214939/selective-determination-of-sulfonamides-from-environmental-water-based-on-magnetic-surface-molecularly-imprinting-technology
#2
Yang Xu, Qi Zhao, Liyan Jiang, Zhengqiang Li, Yanhua Chen, Lan Ding
In the study, a simple and selective method based on magnetic separation technology is presented for the extraction of sulfonamides (SAs) from environmental water, followed by liquid chromatography-tandem mass spectrometry. In this method, magnetic surface molecularly imprinted polymers (Fe3O4@SiO2@MIPs) with super-paramagnetic property and high selectivity toward SAs were developed as magnetic adsorbents. The Fe3O4@SiO2@MIPs were then applied to the selective extraction of SAs from environmental water. The extraction and enrichment were accomplished simultaneously in a single step by simply stirring the mixture of adsorbents and water samples...
February 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28214676/chiral-aminoquinoline-based-fluorescent-ph-probe-with-large-stokes-shift-for-bioimaging
#3
Yong-Sheng Xie, Xin-Ling Zhang, Kun Xie, Yanmei Zhao, Huan Wu, Jidong Yang
The aminoquinoline derivatives, (R)- and (S)-2-phenyl-2-(quinolin-6-ylamino)ethan-1-ol (R-PEO and S-PEO), were synthesized by a tandem one-pot three-step CN coupling method where Smiles rearrangement was the key procedure. The selected compound R-PEO showed a significant fluorescence enhancement with a turn-on ratio over 98-fold and enabled the real time determination of proton concentration in acidic solution. The fluorescence intensity of R-PEO exhibited strong pH-dependent performance with a large Stokes shift (115nm) and responded linearly to minor pH change within the range of 3...
February 12, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28214542/several-hpept1-transported-drugs-are-substrates-of-the-e-coli-proton-coupled-oligopeptide-transporter-ydgr
#4
Bala K Prabhala, Nanda G Aduri, Mazhar Iqbal, Moazur Rahman, Michael Gajhede, Paul R Hansen, Osman Mirza
Proton-dependent oligopeptide transporters (POTs) are secondary active transporters found in all kingdoms of life. POTs utilize the proton electrochemical gradient for the uptake of nutrient dipeptides and tripeptides. The human POT hPepT1 is known to transport a number of drugs. As part of ongoing studies on substrate specificities of POTs from E. coli, our aim in this study was to investigate whether bacterial POTs could also transport these drugs. For this, we selected the common orally administered drugs sulpiride, bestatin, valacyclovir, ampicillin and oseltamivir, that are all transported by hPepT1...
February 15, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28214376/sensitive-quantification-of-coixol-a-potent-insulin-secretagogue-in-scoparia-dulcis-extract-using-high-performance-liquid-chromatography-combined-with-tandem-mass-spectrometry-and-uv-detection
#5
Arslan Ali, Faraz Ul Haq, Qamar Ul Arfeen, Khaga Raj Sharma, Achyut Adhikari, Syed Ghulam Musharraf
Diabetes is a major global health problem which requires new studies for its prevention and control. Scoparia dulcis, an herbal product, is widely used for treatment of diabetes. Recent studies demonstrate coixol as a potent and non-toxic insulin secretagogue from S. dulcis. This study focuses on developing two quantitative methods of coixol in Scoparia dulcis methanol-based extracts. Quantification of coixol was performed using high performance liquid chromatography-tandem mass spectrometry (method 1) and high performance liquid chromatography-ultraviolet detection (method 2) with limit of detection of 0...
February 18, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28214238/high-prevalence-of-low-serum-biologically-active-testosterone-in%C3%A2-older-male-veterans
#6
Christine F Conover, Joshua F Yarrow, Timothy J Garrett, Fan Ye, Eoin P Quinlivan, Darryl F Cannady, Mark D Peterson, Stephen E Borst
OBJECTIVES: Assess the prevalence of hypogonadism in older male Veterans by comparing direct measurements of total testosterone (T) and bioavailable testosterone (BioT) versus indirect BioT values derived from existing and newly developed regression analyses. DESIGN: Cohort study. SETTING: Malcom Randall VA Medical Center, Gainesville, FL. PARTICIPANTS: Community-dwelling male Veterans aged 60 and older (n = 203). MEASUREMENTS: Total T, BioT, albumin, sex hormone-binding globulin (SHBG), and body mass index were evaluated...
February 14, 2017: Journal of the American Medical Directors Association
https://www.readbyqxmd.com/read/28213869/persistence-of-touch-dna-on-burglary-related-tools
#7
Céline M Pfeifer, Peter Wiegand
Experts are increasingly concerned by issues regarding the activity level of DNA stains. A case from our burglary-related casework pointed out the need for experiments regarding the persistence of DNA when more than one person touched a tool handle. We performed short tandem repeat (STR) analyses for three groups of tools: (1) personal and mock owned tools; (2) tools, which were first "owned" by a first user and then handled in a burglary action by a second user; and (3) tools, which were first owned by a first user and then handled in a moderate action...
February 6, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28213654/molecular-epidemiology-of-leptospira-serogroup-pomona-infections-among-wild-and-domestic-animals-in-spain
#8
Z J Arent, C Gilmore, J M San-Miguel Ayanz, L Quevedo Neyra, F J García-Peña
Strains of Leptospira serogroup Pomona are known to cause widespread animal infections in many parts of the world. Forty-three isolates retrieved from domestic animals and wild small mammals suggest that serogroup Pomona is epidemiologically relevant in Spain. This is supported by the high prevalence of serovar Pomona antibodies in livestock and wild animals. In this study, the strains were serologically and genetically characterized in an attempt to elucidate their epidemiology. Serological typing was based on the microscopic agglutination test but molecular typing involved species-specific polymerase chain reaction, restriction endonuclease analysis, and multiple-locus variable-number tandem repeat analysis...
February 17, 2017: EcoHealth
https://www.readbyqxmd.com/read/28213513/runx1-cooperates-with-flt3-itd-to-induce-leukemia
#9
Kira Behrens, Katrin Maul, Nilgün Tekin, Neele Kriebitzsch, Daniela Indenbirken, Vladimir Prassolov, Ursula Müller, Hubert Serve, Jörg Cammenga, Carol Stocking
Acute myeloid leukemia (AML) is induced by the cooperative action of deregulated genes that perturb self-renewal, proliferation, and differentiation. Internal tandem duplications (ITDs) in the FLT3 receptor tyrosine kinase are common mutations in AML, confer poor prognosis, and stimulate myeloproliferation. AML patient samples with FLT3-ITD express high levels of RUNX1, a transcription factor with known tumor-suppressor function. In this study, to understand this paradox, we investigated the impact of RUNX1 and FLT3-ITD coexpression...
February 17, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28213406/mitochondria-regulated-formation-of-endothelial-extracellular-vesicles-shifts-the-mediator-of-flow-induced-vasodilation
#10
Julie K Freed, Matthew J Durand, Brian Robert Hoffmann, John C Densmore, Andrew S Greene, David D Gutterman
In order to examine the effect of endothelial-derived extracellular vesicles (eEVs) on the mechanism of flow-induced dilation (FID) composition, formation, and functional effects on the mechanism of FID were examined from two different eEV subtypes, one produced from ceramide, the other from plasminogen-activator inhibitor 1 (PAI-1). Using videomicroscopy, internal diameter changes in response to increases in flow were measured in human adipose resistance arteries acutely exposed (30min) to eEVs derived from cultured endothelial cells exposed to ceramide or PAI-1...
February 17, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28213295/determination-of-imm-h004-a-novel-neuroprotective-agent-in-rat-plasma-and-brain-tissue-by-liquid-chromatography-tandem-mass-spectrometry
#11
Ziqian Zhang, Xiangmeng Wu, Manman Zhao, Yakun Yang, Yan Wang, Jinping Hu, Baolian Wang, Li Sheng, Yan Li
A rapid and sensitive liquid chromatography-tandem mass spectrometry method for determination of IMM-H004, a novel neuroprotective agent, in rat plasma and brain was developed. Plasma and brain tissue homogenate samples containing IMM-H004 and propranolol (internal standard, IS) were prepared by using a direct protein precipitation of acetonitrile. Separation was carried out in Zorbax SB-C18 column at a flow rate of 0.3mL/min utilizing acetonitrile/water as mobile phases which contain 0.5% formic acid (v/v)...
February 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28213243/multi-residue-analysis-of-36-priority-and-emerging-pollutants-in-marine-echinoderms-holothuria-tubulosa-and-marine-sediments-by-solid-liquid-extraction-followed-by-dispersive-solid-phase-extraction-and-liquid-chromatography-tandem-mass-spectrometry-analysis
#12
J Martín, A Zafra-Gómez, F Hidalgo, A J Ibáñez-Yuste, E Alonso, J L Vilchez
Marine echinoderms are filter-feeding invertebrates widely distributed along the coasts, and which are therefore extensively exposed to anthropogenic xenobiotics. They can serve as good sentinels for monitoring a large variety of contaminants in marine ecosystems. In this context, a multi-residue analytical method has been validated and applied to Holothuria tubulosa specimens and marine sediments for the determination of 36 organic compounds, which belong to some of the most problematic groups of emerging and priority pollutants (perfluoroalkyl compounds, estrogens, parabens, benzophenones, plasticizers, surfactants, brominated flame retardants and alkylphenols)...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28213240/sensitive-and-precise-monitoring-of-phosphatidylethanol-in-human-blood-as-a-biomarker-for-alcohol-intake-by-ultrasound-assisted-dispersive-liquid-liquid-microextraction-combined-with-liquid-chromatography-tandem-mass-spectrometry
#13
Siming Wang, Ruiyue Yang, Fusui Ji, Hongxia Li, Jun Dong, Wenxiang Chen
Phosphatidylethanol (PEth) is a special phospholipid that is only formed in the presence of ethanol, and therefore, serves as a promising biomarker for alcohol intake. In this study, a simple, rapid and precise method based on LC-MS/MS combined with ultrasound-assisted dispersive liquid-liquid microextraction was developed and validated for the measurements of PEth (16:0/18:1, 16:0/18:2, 16:0/16:0, and 18:1/18:1) in human blood. The influences of several variables for sample extraction and MS detection were carefully investigated...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28213231/stable-isotope-labeling-assisted-liquid-chromatography-tandem-mass-spectrometry-for-the-analysis-of-perfluorinated-carboxylic-acids-in-serum-samples
#14
Shijuan Zhang, Zhongyin Ji, Zhiwei Sun, Mingshuang Li, Cuncun Sheng, Mingbo Yue, Yanxin Yu, Guang Chen, Jinmao You
A new stable isotope labeling (SIL) reagent pair, 10-methyl-acridone-2-sulfonohydrazide (MASH) and its deuterated counterpart d3-MASH was synthesized and successfully applied to the analysis of perfluorinated carboxylic acids (PFCAs) in serum samples. The limits of detection (LODs) were in the range of 0.07-0.42μg/L, and the limits of quantitation (LOQs) were in the range of 0.25-1.38μg/L. Besides ionization enhancing effect, MASH also showed excellent fluorescence property. Therefore, the mass spectrometer operation cost was greatly lowered by carrying out parameter optimization experiments on HPLC which is easier to operate and maintain...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28213209/molecularly-imprinted-solid-phase-extraction-method-for-simultaneous-determination-of-seven-nitroimidazoles-from-honey-by-hplc-ms-ms
#15
Xiu-Chun Guo, Zhao-Yang Xia, Hai-Hui Wang, Wen-Yi Kang, Li-Ming Lin, Wen-Qing Cao, Hong-Wei Zhang, Wen-Hui Zhou
In this work, a selective sample cleanup procedure that combined molecular imprinting technique with solid phase extraction was developed for the simultaneous extraction of the seven nitroimidazoles (NMZs) from honey samples. The molecular imprinting polymers for NMZs were prepared through bulk polymerization method using 2-methyl-5-nitroimidazole as template molecule, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross linking agent. The obtained molecular imprinting polymers showed high affinity to template molecule and was used as selective sorbent for simultaneously selective extraction of the seven NMZs from honey matrix...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28213178/the-atypical-excretion-profile-of-meldonium-comparison-of-urinary-detection-windows-after-single-and-multiple-dose-application-in-healthy-volunteers
#16
Christian Görgens, Sven Guddat, Christina Bosse, Hans Geyer, Valentin Pop, Wilhelm Schänzer, Mario Thevis
Following a one-year monitoring program providing unequivocal analytical evidence for a high prevalence in international elite sports, meldonium has been included in the World Anti-Doping Agency's (WADA) list of prohibited substances that came into effect on 1 January 2016. Despite of the polar and hydrophilic nature of the molecule, an unusual long detection window was observed in pilot elimination studies. Consequently, in the present study, urinary excretion profiles after single-dose (5 volunteers, 1×500mg) and multiple-dose oral application (5 volunteers; 2×500mg/day for 6days) were determined in order to facilitate the result management concerning meldonium findings in doping controls...
February 6, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28213161/a-genomic-view-of-short-tandem-repeats
#17
REVIEW
Melissa Gymrek
Short tandem repeats (STRs) are some of the fastest mutating loci in the genome. Tools for accurately profiling STRs from high-throughput sequencing data have enabled genome-wide interrogation of more than a million STRs across hundreds of individuals. These catalogs have revealed that STRs are highly multiallelic and may contribute more de novo mutations than any other variant class. Recent studies have leveraged these catalogs to show that STRs play a widespread role in regulating gene expression and other molecular phenotypes...
February 14, 2017: Current Opinion in Genetics & Development
https://www.readbyqxmd.com/read/28212902/intrauterine-valproate-exposure-is-associated-with-alterations-in-hippocampal-cell-numbers-and-folate-metabolism-in-a-rat-model-of-valproate-teratogenicity
#18
Alexander Semmler, Christian Frisch, Christiane Bleul, Desiree Smith, Laurent Bigler, Jean-Christophe Prost, Henk Blom, Michael Linnebank
PURPOSE: Valproate is one of the most commonly used anticonvulsive drugs. Despite its significant benefits, the teratogenicity of valproate is a relevant problem in the treatment of women of childbearing age. In addition to major congenital malformations, such as neural tube defects, reduced intelligence and attention after intrauterine valproate exposure are reported. Until now the mechanisms of teratogenicity of VPA are poorly understood and concepts how to reduce valproate teratogenicity are lacking...
January 27, 2017: Seizure: the Journal of the British Epilepsy Association
https://www.readbyqxmd.com/read/28212656/using-a-vector-pool-containing-variable-strength-promoters-to-optimize-protein-production-in-yarrowia-lipolytica
#19
Rémi Dulermo, François Brunel, Thierry Dulermo, Rodrigo Ledesma-Amaro, Jérémy Vion, Marion Trassaert, Stéphane Thomas, Jean-Marc Nicaud, Christophe Leplat
BACKGROUND: The yeast Yarrowia lipolytica is an increasingly common biofactory. To enhance protein expression, several promoters have been developed, including the constitutive TEF promoter, the inducible POX2 promotor, and the hybrid hp4d promoter. Recently, new hp4d-inspired promoters have been created that couple various numbers of UAS1 tandem elements with the minimal LEU2 promoter or the TEF promoter. Three different protein-secretion signaling sequences can be used: preLip2, preXpr2, and preSuc2...
February 17, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28212567/ribosomal-dna-status-inferred-from-dna-cloud-assays-and-mass-spectrometry-identification-of-agarose-squeezed-proteins-interacting-with-chromatin-aspic-ms
#20
Kamil Krol, Justyna Jendrysek, Janusz Debski, Marek Skoneczny, Anna Kurlandzka, Joanna Kaminska, Michal Dadlez, Adrianna Skoneczna
Ribosomal RNA-encoding genes (rDNA) are the most abundant genes in eukaryotic genomes. To meet the high demand for rRNA, rDNA genes are present in multiple tandem repeats clustered on a single or several chromosomes and are vastly transcribed. To facilitate intensive transcription and prevent rDNA destabilization, the rDNA-encoding portion of the chromosome is confined in the nucleolus. However, the rDNA region is susceptible to recombination and DNA damage, accumulating mutations, rearrangements and atypical DNA structures...
February 15, 2017: Oncotarget
keyword
keyword
113123
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"