keyword
MENU ▼
Read by QxMD icon Read
search

Tandem

keyword
https://www.readbyqxmd.com/read/29050215/proteomics-analysis-of-bladder-cancer-invasion-targeting-eif3d-for-therapeutic-intervention
#1
Agnieszka Latosinska, Marika Mokou, Manousos Makridakis, William Mullen, Jerome Zoidakis, Vasiliki Lygirou, Maria Frantzi, Ioannis Katafigiotis, Konstantinos Stravodimos, Marie C Hupe, Maciej Dobrzynski, Walter Kolch, Axel S Merseburger, Harald Mischak, Maria G Roubelakis, Antonia Vlahou
Patients with advanced bladder cancer have poor outcomes, indicating a need for more efficient therapeutic approaches. This study characterizes proteomic changes underlying bladder cancer invasion aiming for the better understanding of disease pathophysiology and identification of drug targets. High resolution liquid chromatography coupled to tandem mass spectrometry analysis of tissue specimens from patients with non-muscle invasive (NMIBC, stage pTa) and muscle invasive bladder cancer (MIBC, stages pT2+) was conducted...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29049967/halogenated-fatty-amides-a-brand-new-class-of-disinfection-by-products
#2
Dmitry S Kosyakov, Nikolay V Ul'yanovskii, Mark S Popov, Tomas B Latkin, Albert T Lebedev
An array of similar halogenated nitrogen-containing compounds with elemental composition CnH2nNO2X, CnH2n-2NO2X and CnH2n-1NOX2 (X = Cl, Br; n = 16, 18, 22) was detected in drinking water with high performance liquid chromatography - high resolution mass spectrometry (HPLC-HRMS) method. Compounds of this type were never mentioned among disinfection by-products. Tandem mass spectrometry allowed referring them to halohydrines or dihalogenated fatty amides, the products of conjugated electrophilic addition of halogens to the double bonds of unsaturated fatty amides...
October 6, 2017: Water Research
https://www.readbyqxmd.com/read/29049352/exposure-to-intrauterine-inflammation-alters-metabolomic-profiles-in-the-amniotic-fluid-fetal-and-neonatal-brain-in-the-mouse
#3
Amy G Brown, Natalia M Tulina, Guillermo O Barila, Michael S Hester, Michal A Elovitz
INTRODUCTION: Exposure to prenatal inflammation is associated with diverse adverse neurobehavioral outcomes in exposed offspring. The mechanism by which inflammation negatively impacts the developing brain is poorly understood. Metabolomic profiling provides an opportunity to identify specific metabolites, and novel pathways, which may reveal mechanisms by which exposure to intrauterine inflammation promotes fetal and neonatal brain injury. Therefore, we investigated whether exposure to intrauterine inflammation altered the metabolome of the amniotic fluid, fetal and neonatal brain...
2017: PloS One
https://www.readbyqxmd.com/read/29048990/title-site-specific-glycosylation-profile-of-influenza-a-h1n1-hemagglutinin-through-tandem-mass-spectrometry
#4
Esteban Cruz, Joel Cain, Ben Crossett, Veysel Kayser
The study of influenza virus evolution in humans has revealed a significant role of glycosylation profile alterations in the viral glycoproteins - hemagglutinin (HA) and neuraminidase (NA), in the emergence of both seasonal and pandemic strains. Viral antigenic drift can modify the number and location of glycosylation sites, altering a wide range of biological activities and the antigenic properties of the strain. In view of the key role of glycans in determining antigenicity, elucidating the glycosylation profiles of influenza strains is a requirement towards the development of improved vaccines...
October 19, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29048886/a-mild-hydroaminoalkylation-of-conjugated-dienes-using-a-unified-cobalt-and-photoredox-catalytic-system
#5
Scott M Thullen, Tomislav Rovis
Metallo-photoredox catalysis has redefined the available bond disconnections in the synthetic arsenal. By harnessing the one-electron chemistry of photoredox catalysis in tandem with low-valent cobalt catalysts, new methods by which functionalities may be stitched together become available. Herein we describe the coupling of photoredox-generated α-amino radical species with conjugated dienes using a unified cobalt and iridium catalytic system in order to access a variety of useful homoallylic amines from simple commercially available starting materials...
October 19, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29048855/-determination-of-strobilurin-fungicides-in-fruits-and-their-mass-fragmentation-routes-by-ultra-performance-liquid-chromatography-tandem-mass-spectrometry
#6
Yao Zhou, Huiqin Yang, Yiyin Shi, Jiaxian Chen, Jian Zhu, Xiaojun Deng, Dehua Guo
A method was developed for the simultaneous determination of six strobilurin fungicide (E-metominostrobin, azoxystrobin, kresoxim-methyl, picoxystrobin, pyraclostrobin and trifloxystrobin) residues in orange, banana, apple and pineapple samples by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The fragmentation routes of all the compounds were explained by the aid of a fragment predicting software ACD Lab/MS Fragmenter. The samples were extracted by acetonitrile, then cleaned up by amino solid phase extraction cartridges (SupelClean LC-NH2)...
September 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048854/-determination-of-the-plant-growth-regulators-in-batatas-by-matrix-solid-phase-dispersive-extraction-and-high-performance-liquid-chromatography-with-tandem-mass-spectrometry
#7
Jing Zhang, Huihui Wan, Hua Zhang
An analytical method based on matrix solid-phase dispersive (MSPD) extraction and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established for the determination of forchlorfenuron, 6-benzylaminopurine (6-BA), N-(2-ethylhexyl)-5-norbornene-2,3-dicarboximide (MGK 264) and paclobutrazol in batatas. The samples were dispersed with silica by grinding, and then extracted with methanol. The determination was carried out on a Thermo hypersil GOLD C18 column (150 mm×2.1 mm, 5 μm) in gradient elution with mobile phases of methanol and ammonium formate (5 mmol/L, containing 0...
September 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048845/-determination-of-20-pyrethroid-residues-in-vegetable-foods-by-gas-chromatography-tandem-mass-spectrometry
#8
Li Qu, You Li, Jing Zeng, Yonggang Sheng, Xionghai Yi, Jia Cheng
A method of gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed for the simultaneous determination of 20 pyrethroid residues in vegetable foods. The samples were extracted by acetone-n-hexane (1:1, v/v), cleaned up by activated charcoal column and quantified by external standard method. The correlation coefficients (r(2)) of all the 20 pyrethroid pesticides in the range of 0.005-1.0 mg/L were not less than 0.990. The limits of detection (LODs) were 2.0 μ g/kg when signal/noise (S/N) > 3...
July 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048844/-determination-of-microcystins-in-algal-bloom-samples-by-ultra-performance-liquid-chromatography-tandem-mass-spectrometry-and-elimination-of-their-matrix-effects
#9
Fei Shen, Yanjuan Xu, Sheng Jiang, Bin Zhao, Junzhe Shi, Jing Chen
A rapid and simple method was commonly used for the determination of microcystins by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The reliability, accuracy and sensitivity of LC-MS/MS determination were affected by the matrix effect because of the complex matrices in algal bloom samples. In this study, the matrix interference and matrix effect problems were effectively reduced or eliminated by diluting samples, optimizing gradient elution programs, and reducing injection volumes...
July 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048837/-determination-of-tristyrylphenol-ethoxylates-by-high-performance-liquid-chromatography-tandem-mass-spectrometry
#10
Peng Zhang, Nannan Zhao, Hang Su, Fen Jin, Hua Shao, Maojun Jin, Shanshan Wang, Yongxin She, Lufei Zheng, Jing Wang
A high performance liquid chromatography-tandem mass spectrometry method for the analysis of tristyrylphenol ethoxylates (TSPnEO) was established. The separation effects of TSPnEO in the four liquid chromatography modes, including reversed-phase liquid chromatography column (XBridge C18, 150 mm×2.1 mm, 3.5 μ m), hydrophilic interaction liquid chromatography column (XBridge HILIC, 150 mm×2.1 mm, 3.5 μ m), amide column (XBridge Amide, 150 mm×2.1 mm, 3.5 μ m) and C18 column (XBridge C18, 50 mm×2.1 mm, 5 μ m) combined with silica column (Nova-Pak Silica, 150 mm×2...
July 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048836/-rapid-screening-the-alkaloids-of-poppy-shell-in-hot-pot-condiment-beef-noodle-soup-and-seasoning-by-direct-analysis-in-real-time-tandem-mass-spectrometry
#11
Baile Zhang, Lihong Gao, Yingshuang Xie, Wei Zhou, Xiaofeng Chen, Chunni Lei, Huan Zhang
A direct analysis in real time tandem mass spectrometry (DART-MS/MS) method was established for quickly screening five illegally added alkaloids of poppy shell from the hot pot condiment, beef noodle soup and seasoning. The samples were extracted and purified by acetonitrile, and then injected under the conditions of ionization temperature of 300℃, grid electrode voltage of 150 V and sampling rate of 0.8 mm/s using DART in the positive ion mode. The determination was conducted by tandem mass spectrometry in positive ESI mode under multiple reaction monitoring (MRM) mode...
July 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048835/-determination-of-pyriminobac-methyl-and-bispyribac-sodium-residues-in-rice-by-liquid-chromatography-tandem-mass-spectrometry-based-on-quechers
#12
Meiling Qin, Shuangshuang Chai, Youning Ma, Huanhuan Gao, Hantong Zhang, Qiao He
A method was developed for the determination of pyriminobac-methyl and bispyribac-sodium residues in rice by liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with modified QuEChERS. The samples were extracted with acidified acetonitrile, and then purified by octadecylsilane bonded silica (C18) adsorbents. The analytes were separated on a ZORBAX SB C18 column through a gradient elution using 0.1% (v/v) aqueous formic acid aqueous containing 5 mmol/L ammonium acetate and acetonitrile as mobile phases...
July 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048829/-rapid-determination-of-spirotetramat-and-its-metabolite-residues-in-milk-by-ultra-performance-liquid-chromatography-tandem-mass-spectrometry
#13
Tinghui Yi, Bobin Tang, Yun Zou, Cunxian Xi, Guomin Wang
A method for the rapid and simultaneous determination of spirotetramat (BYI08330) and its four metabolites in milk was developed with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The samples were extracted with acetonitrile for precipitating proteins, degreased with hexane, and analyzed by UPLC-MS/MS in positive electrospray ionization and multiple reaction monitoring (MRM) mode. Good linear relationships between peak areas and mass concentrations of the analytes were obtained in the range of 0...
July 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048827/-simultaneous-determination-of-11-mycotoxins-in-feeds-by-high-performance-liquid-chromatography-tandem-mass-spectrometry
#14
Yunji Wei, Min Feng, Zhenyi Zhu, Jian He, Jinrong Shen, Zhenghe He, Xian Qin, Liying Zhou
A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the simultaneous determination of 11 mycotoxins in feeds. The samples were extracted with acetonitrile, then cleaned up by multifunctional purification column filler and PRIME HLB solid phase extraction column. The 11 mycotoxins were separated on an Agilent Zorbax SB-C18 column (150 mm×2.1 mm, 3.5 μ m) with gradient elution program, and methanol-5 mmol/L ammonium acetate solution containing 0.1%(v/v) formic acid were used as mobile phases...
August 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048822/-determination-of-pyrethroid-pesticides-in-tea-by-series-two-solid-phase-extraction-columns-cleanup-and-gas-chromatography-tandem-mass-spectrometry
#15
Li Liu, Qi Zhang, Haiyan Wang, Feng Feng, Hairong Cao, Zhen Yang, Zhijun Ke, Xiyao Chen
A rapid determination method of three pyrethroid pesticides (cyfluthrin, cypermethrin and fenvalerate) in tea was developed by series two-solid phase extraction-column cleanup and external standard method coupled with gas chromatography-tandem mass spectrometry (GC-MS/MS) in 30 min. The pesticide residues in tea were extracted with acetonitrile, and cleaned up by Carb/NH2 and SLH solid-phase extraction columns. After filtration, the target compounds were analyzed by GC-MS and quantified by the external standard method...
August 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048816/-determination-of-nicotine-and-cotinine-in-urine-by-hydrophilic-interaction-chromatography-tandem-mass-spectrometry
#16
Mingxin Li, Qian Wang, Jing Zhu, Lin Ren, Li Yong, Xiaoli Zou
The analytical method of nicotine and cotinine in human urine with hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS) was established. After the urine sample containing nicotine-d4 and cotinine-d3 isotope internal standards being diluted with water, the filtrate was introduced into ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for analysis. Separation was performed on an ACQUITY UPLC(®) BEH HILIC column (50 mm×3.0 mm, 1.7 μ m), in which methanol and 0...
August 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048815/-simultaneous-rapid-determination-of-81-glucocorticoids-in-cosmetics-by-dispersive-solid-phase-extraction-and-liquid-chromatography-tandem-mass-spectrometry
#17
Huitai Luo, Xiaolan Huang, Huiqin Wu, Qiuyan Zhang, Zhixin Zhu, Fang Huang, Xiaoshan Lin, Yefen Ma, Xin Deng
A novel method was developed for the simultaneous rapid determination of 81 illegally added glucocorticoids (GCs) in cosmetics using dispersive-solid phase extraction (d-SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The analytes were extracted by acetonitrile after dispersing with water, and then purified using the C18 and primary secondary amine (PSA). The chromatographic separations were performed on a Poroshell 120 PFP column (100 mm×2.1 mm, 2.7 μ m) under multiple chromatographic retention modes...
August 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048813/-determination-of-three-tranquillizer-residues-in-animal-foods-by-quechers-high-performance-liquid-chromatography-tandem-mass-spectrometry
#18
You Zou, Linzhi Shao, Simin Chen, Shaolun Ouyang, Feng Lin
A method for simultaneous determination of chlorpromazine, diazepam and metolazone residues in porcine muscle, fish, liver and kidney was developed using QuEChERS and HPLC-MS/MS technique. The samples were extracted with ethyl acetate and cleaned up with C18, N-propylethylendiamine (PSA) and NH2 sorbents after using Na2SO4 as dehydrating agent. The analytes were separated by a special C18 column, Atlantis T3, and gradiently eluted with a mixed solution of 5 mmol/L formic acid and acetonitrile at a flow rate of 0...
August 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048812/-rapid-and-simultaneous-determination-of-seven-microcystins-in-fish-meat-by-liquid-chromatography-tandem-mass-spectrometry-coupled-with-pass-through-solid-phase-extraction
#19
Shiyan Li, Yang Wang, Dingnan Wang, Hongxi Wu, Xueyan Ding, Yiwei Cui, Qing Shen
An analytical method was developed for the simultaneous and rapid determination of seven microcystins in fish meat by liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with pass-through solid phase extraction (SPE). The samples were extracted with methanol-water (90:10, v/v) after heat treatment by water bath at 80℃, and then cleaned up with an Oasis PRiME HLB pass-through SPE column. The samples were analyzed directly on a Waters XSelect HSS T3 column using 0.1% (v/v) aqueous formic acid and acidified acetonitrile (0...
August 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048810/-simultaneous-determination-of-15-polycyclic-aromatic-hydrocarbons-in-cigarette-filter-by-gas-chromatography-tandem-mass-spectrometry
#20
Xiaotao Zhang, Li Zhang, Yibin Ruan, Weiwei Wang, Houwei Ji, Qiang Wan, Fucheng Lin, Jian Liu
A method for the simultaneous determination of 15 polycyclic aromatic hydrocarbons in cigarette filter was developed by isotope internal standard combined with gas chromatography-tandem mass spectrometry. The cigarette filters were extracted with dichloromethane, and the extract was filtered with 0.22 μm organic phase membrane. The samples were isolated by DB-5MS column (30 m×0.25 mm, 0.25 μm) and detected using multiple reaction monitoring mode of electron impact source under positive ion mode. The linearities of the 15 polycyclic aromatic hydrocarbons (acenapthylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, ben[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenzo[a,h]anthracene, benzo[g,h,i]perylene and indeno[1,2,3-c,d]pyrene) were good, and the correlation coefficients (R(2)) ranged from 0...
October 8, 2017: Se Pu, Chinese Journal of Chromatography
keyword
keyword
113123
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"