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https://www.readbyqxmd.com/read/27225843/chromatin-dynamics-during-dna-replication
#1
Raz Bar-Ziv, Yoav Voichek, Naama Barkai
Chromatin is composed of DNA and histones, which provide a unified platform for regulating DNA-related processes, mostly through their post-translational modification. During DNA replication, histone arrangement is perturbed, first to allow progression of DNA polymerase and then during repackaging of the replicated DNA. To study how DNA replication influences the pattern of histone modification, we followed the cell-cycle dynamics of 10 histone marks in budding yeast. We find that histones deposited on newly replicated DNA are modified at different rates: While some marks appear immediately upon replication (e...
September 2016: Genome Research
https://www.readbyqxmd.com/read/19412618/gal4-induces-transcriptionally-active-puff-in-the-absence-of-dsaga-and-atac-specific-chromatin-acetylation-in-the-drosophila-melanogaster-polytene-chromosome
#2
Anita Ciurciu, István Tombácz, Cristina Popescu, Imre Boros
Puffs in the polytene chromosome of Drosophila melanogaster are characteristic of sites of high-level active transcription which can be observed directly under the microscope. We studied the dependence of puff formation on chromatin modifications at a site where a GAL4-inducible transgene is located in the 61C7 cytological region. Immunostaining of salivary gland polytene chromosomes indicated no increase of either dSAGA-specific histone H3 lysine 14, or ATAC-specific histone H4 lysine 5 and 12 acetylation in the puffed region...
August 2009: Chromosoma
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