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https://www.readbyqxmd.com/read/29145635/trex-a-web-portal-for-exploration-of-trna-derived-fragments-in-arabidopsis-thaliana
#1
Agnieszka Thompson, Andrzej Zielezinski, Patrycja Plewka, Maciej Szymanski, Przemyslaw Nuc, Zofia Szweykowska-Kulinska, Artur Jarmolowski, Wojciech M Karlowski
tRNA-derived fragments (tRFs) constitute a new class of short regulatory RNAs that are a product of nascent or mature tRNA processing. tRF sequences have been identified in all domains of life; however, most published research pertains to human, yeast and some bacterial organisms. Despite growing interest in plant tRFs and accumulating evidence of their function in plant development and stress responses, no public, web-based repository dedicated to these molecules is currently available. Here, we introduce tRex (http://combio...
November 14, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29143817/greater-post-neolithic-wealth-disparities-in-eurasia-than-in-north-america-and-mesoamerica
#2
Timothy A Kohler, Michael E Smith, Amy Bogaard, Gary M Feinman, Christian E Peterson, Alleen Betzenhauser, Matthew Pailes, Elizabeth C Stone, Anna Marie Prentiss, Timothy J Dennehy, Laura J Ellyson, Linda M Nicholas, Ronald K Faulseit, Amy Styring, Jade Whitlam, Mattia Fochesato, Thomas A Foor, Samuel Bowles
How wealth is distributed among households provides insight into the fundamental characters of societies and the opportunities they afford for social mobility. However, economic inequality has been hard to study in ancient societies for which we do not have written records, which adds to the challenge of placing current wealth disparities into a long-term perspective. Although various archaeological proxies for wealth, such as burial goods or exotic or expensive-to-manufacture goods in household assemblages, have been proposed, the first is not clearly connected with households, and the second is confounded by abandonment mode and other factors...
November 15, 2017: Nature
https://www.readbyqxmd.com/read/29143703/dose-response-evaluation-of-the-standardized-ileal-digestible-tryptophan-lysine-ratio-to-maximize-growth-performance-of-growing-finishing-gilts-under-commercial-conditions
#3
M A D Gonçalves, M D Tokach, N M Bello, K J Touchette, R D Goodband, J M DeRouchey, J C Woodworth, S S Dritz
Environmental regulations as well as economic incentives have resulted in greater use of synthetic amino acids in swine diets. Tryptophan is typically the second limiting amino acid in corn-soybean meal-based diets. However, using corn-based co-products emphasizes the need to evaluate the pig's response to increasing Trp concentrations. Therefore, the objective of these studies was to evaluate the dose-response to increasing standardized ileal digestible (SID) Trp : Lys on growth performance of growing-finishing gilts housed under large-scale commercial conditions...
November 16, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29141714/falciparum-malaria-outbreak-in-sabah-linked-to-an-immigrant-rubber-tapper
#4
Saffree Mohammad Jeffree, Kamruddin Ahmed, Nazarudin Safian, Rohaizat Hassan, Omar Mihat, Khamisah Awang Lukman, Shamsul Bahari Shamsudin, Fadzilah Kamaludin
Sabah is a Malaysian state situated in the northern part of Borneo, and it is endemic for malaria. The incidence of malaria is the lowest (0.05/1,000 population) in Penampang districts of Sabah. In June 26, 2012, two Plasmodium falciparum malaria cases were notified to public health department from a village in Penampang. Immediate investigation was initiated to identify the risk factors and to institute control measures. We performed active case finding by asking household members of all houses in the village regarding malaria symptoms and by examining blood smears...
November 6, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29137946/distinct-environmental-enrichment-protocols-reduce-anxiety-but-differentially-modulate-pain-sensitivity-in-rats
#5
Louise Faggionato Kimura, Vânia Gomes de Moura Mattaraia, Gisele Picolo
Environmental enrichment (EE) can alter anxiety as well as perception of nociceptive stimuli, suggesting a relationship between well-being and analgesia. Considering that, we aimed to evaluate the influence of different EE types on anxiety and peripheral pain sensibility of male Wistar rats. Animals were submitted to two different types of EE: On the first one, animals were housed in standard size cages after weaning and received three different objects, one type per week, on a regular basis (simple EE - sEE)...
November 11, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/29137939/in-silico-identification-and-in-vivo-characterization-of-small-molecule-therapeutic-hypothermia-mimetics
#6
Claire Coderch, María Díaz de Cerio, Jose María Zapico, Rafael Peláez, Ignacio M Larrayoz, Ana Ramos, Alfredo Martínez, Beatriz de Pascual-Teresa
Hypothermia has been proved to have a beneficial effect on several pathologies. CIRBP is one of the so termed cold-shock proteins involved in this process. In this work, we have detected small molecules capable of modulating the activity of CIRBP in the absence of a cold stimulus, by High Throughput Virtual Screening (HTVS) of the Diversity Set IV of the NCI and 15 compounds of our in-house data base. Fifteen compounds were selected from the HTVS to carry out a second screening through a cell-based Western blot assay...
October 31, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29137558/testing-a-web-based-trained-peer-model-to-build-capacity-for-evidence-based-practices-in-community-mental-health-systems
#7
Ramaris E German, Abby Adler, Sarah A Frankel, Shannon Wiltsey Stirman, Paola Pinedo, Arthur C Evans, Aaron T Beck, Torrey A Creed
OBJECTIVE: Use of expert-led workshops plus consultation has been established as an effective strategy for training community mental health (CMH) clinicians in evidence-based practices (EBPs). Because of high rates of staff turnover, this strategy inadequately addresses the need to maintain capacity to deliver EBPs. This study examined knowledge, competency, and retention outcomes of a two-phase model developed to build capacity for an EBP in CMH programs. METHODS: In the first phase, an initial training cohort in each CMH program participated in in-person workshops followed by expert-led consultation (in-person, expert-led [IPEL] phase) (N=214 clinicians)...
November 15, 2017: Psychiatric Services: a Journal of the American Psychiatric Association
https://www.readbyqxmd.com/read/29134026/type-material-of-acanthocephala-nematoda-and-other-non-helminths-phyla-cnidaria-annelida-and-arthropoda-housed-in-the-helminthological-collection-of-the-oswaldo-cruz-institute-fiocruz-chioc-rio-de-janeiro-brazil-from-1979-to-2016
#8
Daniela A Lopes, Delir Corrêa Gomes, Marcelo Knoff
The third part of the catalogue of type material in the Helminthological Collection of the Oswaldo Cruz Institute/FIOCRUZ (CHIOC), comprising types deposited between 1979 and 2016, is presented to complement the first list of all types that was published in 1979. This part encompasses Acanthocephala, Nematoda and the other non-helminth phyla Cnidaria, Annelida, and Arthropoda. Platyhelminthes was covered in the first (Monogenoidea) and second (Rhabditophora Trematoda and Cestoda) parts of the catalogue published in September 2016 and March 2017, respectively...
2017: ZooKeys
https://www.readbyqxmd.com/read/29133313/site-specific-cancer-mortality-inequalities-by-employment-and-occupational-groups-a-cohort-study-among-belgian-adults-2001-2011
#9
Katrien Vanthomme, Laura Van den Borre, Hadewijch Vandenheede, Paulien Hagedoorn, Sylvie Gadeyne
OBJECTIVE: This study probes into site-specific cancer mortality inequalities by employment and occupational group among Belgians, adjusted for other indicators of socioeconomic (SE) position. DESIGN: This cohort study is based on record linkage between the Belgian censuses of 1991 and 2001 and register data on emigration and mortality for 01/10/2001 to 31/12/2011. SETTING: Belgium. PARTICIPANTS: The study population contains all Belgians within the economically active age (25-65 years) at the census of 1991...
November 12, 2017: BMJ Open
https://www.readbyqxmd.com/read/29132960/influenza-derived-peptides-cross-react-with-allergens-and-provide-asthma-protection
#10
Chrysanthi Skevaki, Christoph Hudemann, Mikhail Matrosovich, Christian Möbs, Sinu Paul, Andreas Wachtendorf, Bilal Alashkar Alhamwe, Daniel P Potaczek, Stefanie Hagner, Diethard Gemsa, Holger Garn, Alessandro Sette, Harald Renz
BACKGROUND: The hygiene hypothesis is the leading concept to explain the current asthma epidemic, which is built on the observation that a lack of bacterial contact early in life induces allergic Th2 immune responses. OBJECTIVE: Since little is known about the contribution of respiratory viruses in this context, we evaluated the effect of prior influenza-infection on the development of allergic asthma. METHODS: Mice were infected with influenza and once recovered, subjected to an ovalbumin or house dust mite-induced experimental asthma protocol...
November 10, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29131344/aerrow-a-probe-format-graphite-calorimeter-for-absolute-dosimetry-of-high-energy-photon-beams-in-the-clinical-environment
#11
James Renaud, Arman Sarfehnia, Julien Bancheri, Jan Seuntjens
In this work, the design, operation, initial experimental evaluation, and characterization of a small-scale graphite calorimeter probe - herein referred to as the Aerrow - developed for routine use in the clinical environment, is described. Similar in size and shape to a Farmer type cylindrical ionization chamber, the Aerrow represents the first translation of calorimetry intended for direct use by clinical physicists in the radiotherapy clinic METHODS: Based on a numerically-optimized design obtained in previous work, a functioning Aerrow prototype capable of two independent modes of operation (quasi-adiabatic and isothermal) was constructed in-house...
November 13, 2017: Medical Physics
https://www.readbyqxmd.com/read/29129432/occurrence-and-human-exposure-to-brominated-and-organophosphorus-flame-retardants-via-indoor-dust-in-a-brazilian-city
#12
Joyce Cristale, Tiago Gomes Aragão Belé, Silvia Lacorte, Mary Rosa Rodrigues de Marchi
Indoor dust is considered an important human exposure route to flame retardants (FRs), which has arised concern due the toxic properties of some of these substances. In this study, ten organophosphorus flame retardants (OPFRs), eight polybrominated diphenyl ethers (PBDEs) and four new brominated flame retardants (NBFRs) were determined in indoor dust from different places in Araraquara-SP (Brazil). The sampled places included houses, apartments, offices, primary schools and cars. The analysis of the sample extracts was performed by gas chromatography coupled to mass spectrometry and two ionization techniques were used (electron ionization - EI; electron capture negative ionization - ECNI)...
November 9, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29126026/legacy-and-novel-brominated-flame-retardants-in-indoor-dust-from-beijing-china-occurrence-human-exposure-assessment-and-evidence-for-pbdes-replacement
#13
Jiandi Wang, Yuwei Wang, Zhixiong Shi, Xianqing Zhou, Zhiwei Sun
Levels of legacy brominated flame retardants (BFRs), including tetrabromobisphenol A (TBBPA), hexabromocyclododecane isomers (HBCDs) and polybrominated diphenyl ethers (PBDEs), and six currently used novel BFRs were determined in house dust and office dust collected from a community in Beijing, China. This is the first study where the three kinds of legacy BFRs and novel BFRs were all measured in dust samples from China. HPLC-MS/MS was used for the detection of TBBPA and HBCDs, and the other BFRs were tested on a GC-NCI/MS...
November 7, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29124425/steroid-hormones-in-environmental-matrices-extraction-method-comparison
#14
Gangadhar Andaluri, Rominder P S Suri, Kendon Graham
The U.S. Environmental Protection Agency (EPA) has developed methods for the analysis of steroid hormones in water, soil, sediment, and municipal biosolids by HRGC/HRMS (EPA Method 1698). Following the guidelines provided in US-EPA Method 1698, the extraction methods were validated with reagent water and applied to municipal wastewater, surface water, and municipal biosolids using GC/MS/MS for the analysis of nine most commonly detected steroid hormones. This is the first reported comparison of the separatory funnel extraction (SFE), continuous liquid-liquid extraction (CLLE), and Soxhlet extraction methods developed by the U...
November 9, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29119691/the-detection-of-porcine-circovirus-3-in-guangxi-china
#15
S Wen, W Sun, Z Li, X Zhuang, G Zhao, C Xie, M Zheng, J Jing, P Xiao, M Wang, J Han, J Ren, H Liu, H Lu, N Jin
Porcine circovirus type 3 (PCV3) is a novel circovirus that was firstly detected in the USA. PCV3 is associated with porcine dermatitis and nephropathy syndrome (PDNS), reproductive failure and cardiac and multisystemic inflammation. Latterly, PCV3 was detected in Guangxi, China. Forty-one of 108 (37.96%) samples and nine of 47 (19.14%) samples were PCV3 positive in pig farms and pig slaughter houses, respectively. Three PCV3 strains were sequenced and designated PCV3-China/GX2016-1, PCV3-China/GX2016-2 and PCV3-China/GX2016-3...
November 8, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29118030/large-scale-low-cost-ngs-library-preparation-using-a-robust-tn5-purification-and-tagmentation-protocol
#16
Bianca P Hennig, Lars Velten, Ines Racke, Chelsea Szu Tu, Matthias Thoms, Vladimir Rybin, Hüseyin Besir, Kim Remans, Lars M Steinmetz
Efficient preparation of high quality sequencing libraries that represent well the biological sample is a key step for using next-generation sequencing in research. Tn5 enables fast, robust, and highly efficient processing of limited input material while scaling to the parallel processing of hundreds of samples. Here, we present a robust Tn5 transposase purification strategy based on an N-terminal His6-Sumo3 tag. We demonstrate that libraries prepared with our in-house Tn5 are of the same quality as those processed with a commercially available kit (Nextera XT), while they reduce the cost of large-scale experiments dramatically...
November 8, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29117180/the-additional-benefit-of-residual-spraying-and-insecticide-treated-curtains-for-dengue-control-over-current-best-practice-in-cuba-evaluation-of-disease-incidence-in-a-cluster-randomized-trial-in-a-low-burden-setting-with-intensive-routine-control
#17
Maria Eugenia Toledo, Veerle Vanlerberghe, Julio Popa Rosales, Mayelin Mirabal, Pedro Cabrera, Viviana Fonseca, Tania Gómez Padrón, Mirtha Pérez Menzies, Domingo Montada, Patrick Van der Stuyft
BACKGROUND: Aedes control interventions are considered the cornerstone of dengue control programmes, but there is scarce evidence on their effect on disease. We set-up a cluster randomized controlled trial in Santiago de Cuba to evaluate the entomological and epidemiological effectiveness of periodical intra- and peri-domiciliary residual insecticide (deltamethrin) treatment (RIT) and long lasting insecticide treated curtains (ITC). METHODOLOGY/PRINCIPAL FINDINGS: Sixty three clusters (around 250 households each) were randomly allocated to two intervention (RIT and ITC) and one control arm...
November 8, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29116377/-effectiveness-of-preventive-house-visits-for-elderly-people-systematic-assessment-of-current-literature
#18
REVIEW
Jörg-Christian Renz, Matthias Meinck
BACKGROUND: Preventive home visits (PHV) document health resources and risks in the domestic environment in order to derive preventive recommendations. An earlier review did not yield reliable proof of their efficacy for the elderly; however, PHV are used increasingly more as a municipal service in Germany. OBJECTIVE: This article evaluates the efficacy of PHV by means of evidence-based criteria. MATERIAL AND METHODS: Independent of the reason, randomized controlled studies (RCT) and systematic reviews on PHV in the elderly (60+ years) that were published after 2004 were identified by means of searching pertinent literature databases...
November 7, 2017: Zeitschrift Für Gerontologie und Geriatrie
https://www.readbyqxmd.com/read/29114863/american-hybrid-donald-trump-and-the-strange-merger-of-populism-and-plutocracy
#19
Paul Pierson
Any effort to situate Trump's ascendance in the broader currents of cross-national developments, or in the longer course of American political development, must begin by recognizing it as a curious hybrid of populism and plutocracy. Although American right-wing populism has real social roots, it has long been nurtured by powerful elites seeking to undercut support for modern structures of economic regulation and the welfare state. American political institutions offered a distinctive opportunity for a populist figure to draw on this fury to first capture the nomination of the GOP, and from that position to ascend to the White House...
November 2017: British Journal of Sociology
https://www.readbyqxmd.com/read/29111480/draft-genome-sequences-of-two-fluoroquinolone-resistant-ctx-m-15-producing-escherichia-coli-st90-st23-complex-isolated-from-a-calf-and-a-dairy-cow-in-south-america
#20
Luciana Sartori, Miriam R Fernandes, Susan Ienne, Tiago A de Souza, Lilian Gregory, Louise Cerdeira, Nilton Lincopan
OBJECTIVES: Farm animals have been recognised as important carriers and reservoirs of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. The aim of this study was to report the draft genome sequences of two multidrug-resistant (MDR) CTX-M-15-producing E. coli strains (47VL and 13B) isolated from different bovine hosts (a calf and a dairy cow), housed separately in a commercial dairy farm in Brazil. METHODS: Total genomic DNA of the E. coli isolates was sequenced using an Illumina MiSeq paired-end 300-bp sequencing platform...
October 31, 2017: Journal of Global Antimicrobial Resistance
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