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https://www.readbyqxmd.com/read/27890797/3-bromopyruvate-treatment-induces-alterations-of-metabolic-and-stress-related-pathways-in-glioblastoma-cells
#1
Davide Chiasserini, Magdalena Davidescu, Pier Luigi Orvietani, Federica Susta, Lara Macchioni, Maya Petricciuolo, Emilia Castigli, Rita Roberti, Luciano Binaglia, Lanfranco Corazzi
: Glioblastoma (GBM) is the most common and aggressive brain tumours of adults. The metabolic phenotype of GBM cells is highly dependent on glycolysis; therefore, therapeutic strategies aimed at interfering with glycolytic pathways are under consideration. 3-Bromopyruvate (3BP) is a potent antiglycolytic agent, with a variety of targets and possible effects on global cell metabolism. Here we analyzed the changes in protein expression on a GBM cell line (GL15 cells) caused by 3BP treatment using a global proteomic approach...
November 23, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27883075/direct-electrical-control-of-igg-conformation-and-functional-activity-at-surfaces
#2
Paola Ghisellini, Marialuisa Caiazzo, Andrea Alessandrini, Roberto Eggenhöffner, Massimo Vassalli, Paolo Facci
We have devised a supramolecular edifice involving His-tagged protein A and antibodies to yield surface immobilized, uniformly oriented, IgG-type, antibody layers with Fab fragments exposed off an electrode surface. We demonstrate here that we can affect the conformation of IgGs, likely pushing/pulling electrostatically Fab fragments towards/from the electrode surface. A potential difference between electrode and solution acts on IgGs' charged aminoacids modulating the accessibility of the specific recognition regions of Fab fragments by antigens in solution...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27871938/the-role-of-gpi-anchored-axonal-glycoproteins-in-neural-development-and-neurological-disorders
#3
REVIEW
Gianfranco Gennarini, Antonella Bizzoca, Sabrina Picocci, Daniela Puzzo, Patrizia Corsi, Andrew J W Furley
This review article focuses on the Contactin (CNTN) subset of the Immunoglobulin supergene family (IgC2/FNIII molecules), whose components share structural properties (the association of Immunoglobulin type C2 with Fibronectin type III domains), as well as a general role in cell contact formation and axonal growth control. IgC2/FNIII molecules include 6 highly related components (CNTN 1-6), associated with the cell membrane via a Glycosyl Phosphatidyl Inositol (GPI)-containing lipid tail. Contactin 1 and Contactin 2 share ~50 (49...
November 18, 2016: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/27866936/current-applications-and-different-approaches-for-microbial-l-asparaginase-production
#4
REVIEW
Jorge Javier Muso Cachumba, Felipe Antonio Fernandes Antunes, Guilherme Fernando Dias Peres, Larissa Pereira Brumano, Júlio César Dos Santos, Silvio Silvério Da Silva
l-asparaginase (EC 3.5.1.1) is an enzyme that catalysis mainly the asparagine hydrolysis in l-aspartic acid and ammonium. This enzyme is presented in different organisms, such as microorganisms, vegetal, and some animals, including certain rodent's serum, but not unveiled in humans. It can be used as important chemotherapeutic agent for the treatment of a variety of lymphoproliferative disorders and lymphomas (particularly acute lymphoblastic leukemia (ALL) and Hodgkin's lymphoma), and has been a pivotal agent in chemotherapy protocols from around 30 years...
October 27, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27854256/natural-deep-eutectic-solvents-nades-as-a-tool-for-bioavailability-improvement-pharmacokinetics-of-rutin-dissolved-in-proline-glycine-after-oral-administration-in-rats-possible-application-in-nutraceuticals
#5
Marta Faggian, Stefania Sut, Beatrice Perissutti, Valeria Baldan, Iztok Grabnar, Stefano Dall'Acqua
There is a need for innovation in plant-derived pharmaceuticals, food supplements and nutraceutical products regarding the use of more eco-sustainable solvents for their extraction. Furthermore, the poor oral bioavailability of several phytochemicals with health promoting effects stimulates the research in the field of pharmaceutical formulations. Natural Deep Eutectic Solvents (NADES) are formed by natural compounds, and can be considered as future solvents being especially useful for the preparation of nutraceuticals and food-grade extracts...
November 14, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27815287/microbial-and-viral-like-rhodopsins-present-in-coastal-marine-sediments-from-four-polar-and-subpolar-regions
#6
J L López, M Golemba, E Hernández, M Lozada, H M Dionisi, J Jansson, J Carroll, L Lundgren, S Sjöling, W P Mac Cormack
Rhodopsins are broadly distributed. In this work we analyzed 23 metagenomes corresponding to marine sediment samples from four regions which share cold climate conditions (Norway; Sweden; Argentina and Antarctica). In order to investigate the genes evolution of viral-rodopsins, an initial set of 6224 bacterial rhodopsins sequences according COG5524 were retrieved from the 23 metagenomes. After selection by the presence of transmembrane domains and alignment 123 viral (51) and non-viral (72) sequences (>50 aminoacids) were finally included in further analysis...
November 3, 2016: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/27770947/molecular-level-interactions-in-brushite-aminoacids-composites
#7
Alberto Lagazzo, Fabrizio Barberis, Cristina Carbone, Gianguido Ramis, Elisabetta Finocchio
The interaction of aminoacids (Glycine, Proline, Lysine) with brushite based bone cements has been investigated by several techniques (FTIR spectroscopy, Thermogravimetry-TG, Scanning Electron Microscopy-SEM, mechanical properties studies), with the aim to elucidate the properties of the resulting composite materials and the interaction occurring at molecular level between the inorganic matrix and the organic moieties. Brushite phase is predominantly obtained also in the presence of aminoacids added during preparation of the bone cement...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27752253/cytological-profile-of-antibacterial-ftsz-inhibitors-and-synthetic-peptide-mciz
#8
Lidia Araújo-Bazán, Laura B Ruiz-Avila, David Andreu, Sonia Huecas, José M Andreu
Cell division protein FtsZ is the organizer of the cytokinetic ring in almost all bacteria and a target for the discovery of new antibacterial agents that are needed to counter widespread antibiotic resistance. Bacterial cytological profiling, using quantitative microscopy, is a powerful approach for identifying the mechanism of action of antibacterial molecules affecting different cellular pathways. We have determined the cytological profile on Bacillus subtilis cells of a selection of small molecule inhibitors targeting FtsZ on different binding sites...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27718395/lichenysin-geminal-amino-acid-based-surfactants-synergistic-action-of-an-unconventional-antimicrobial-mixture
#9
Jonathan Coronel-León, Aurora Pinazo, Lourdes Pérez, Mª José Espuny, Ana Mª Marqués, Angeles Manresa
Recently it has been demonstrated that catanionic mixtures of oppositely charged surfactants have improved physicochemical-biological properties compared to the individual components. Isotherms of mixtures of an anionic biosurfactant (lichenysin) and a cationic aminoacid surfactant (C3(LA)2) indicate a strong interaction suggesting the formation of a new "pseudo-surfactant". The antimicrobial properties of the mixture lichenysin and C3(LA)2 M80:20, indicate a synergistic effect of the components. The mechanism of action on the bacterial envelope was assessed by flow cytometry and Transmission Electron Microscopy...
October 4, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27683583/selenium-biofortification-in-radish-enhances-nutritional-quality-via-accumulation-of-methyl-selenocysteine-and-promotion-of-transcripts-and-metabolites-related-to-glucosinolates-phenolics-and-amino-acids
#10
Michela Schiavon, Chiara Berto, Mario Malagoli, Annarita Trentin, Paolo Sambo, Stefano Dall'Acqua, Elizabeth A H Pilon-Smits
Two selenium (Se) fertilization methods were tested for their effects on levels of anticarcinogenic selenocompounds in radish (Raphanus sativus), as well as other nutraceuticals. First, radish was grown on soil and foliar selenate applied 7 days before harvest at 0, 5, 10, and 20 mg Se per plant. Selenium levels were up to 1200 mg Se/kg DW in leaves and 120 mg Se/kg DW in roots. The thiols cysteine and glutathione were present at 2-3-fold higher levels in roots of Se treated plants, and total glucosinolate levels were 35% higher, due to increases in glucoraphanin...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27683036/proteomic-analysis-uncovers-common-effects-of-ifn-%C3%AE-and-il-27-on-the-hla-class-i-antigen-presentation-machinery-in-human-cancer-cells
#11
Andrea Petretto, Grazia Carbotti, Elvira Inglese, Chiara Lavarello, Maria Pia Pistillo, Valentina Rigo, Michela Croce, Luca Longo, Stefania Martini, Paola Vacca, Silvano Ferrini, Marina Fabbi
IL-27, a member of the IL-12-family of cytokines, has shown anti-tumor activity in several pre-clinical models due to anti-proliferative, anti-angiogenic and immune-enhancing effects. On the other hand, IL-27 demonstrated immune regulatory activities and inhibition of auto-immunity in mouse models. Also, we reported that IL-27, similar to IFN-γ, induces the expression of IL-18BP, IDO and PD-L1 immune regulatory molecules in human cancer cells. Here, a proteomic analysis reveals that IL-27 and IFN-γ display a broad overlap of functions on human ovarian cancer cells...
September 24, 2016: Oncotarget
https://www.readbyqxmd.com/read/27671515/processes-of-plasma-protein-n-homocysteinylation-in-multiple-sclerosis
#12
A Jamroz-Wiśniewska, J Bełtowski, H Bartosik-Psujek, G Wójcicka, K Rejdak
BACKGROUND: Homocysteine thiolactone (HTL) is a cyclic thioester of homocysteine (Hcy) contributing to the toxicity of this amino acid. HTL spontaneously reacts with protein lysine residues leading to altered properties of target proteins and induction of immune response. HTL is hydrolyzed to Hcy by plasma enzyme, paraoxonase 1 (PON1). Although both Hcy and PON1 may be involved in the pathogenesis of multiple sclerosis (MS), protein modification by HTL in this disease has not been studied so far...
October 14, 2016: International Journal of Neuroscience
https://www.readbyqxmd.com/read/27645741/bioanalytical-application-of-amino-acid-detection-by-capillary-electrophoresis
#13
Daniela Fico, Antonio Pennetta, Giuseppe E De Benedetto
This chapter illustrates the usefulness of capillary electrophoresis (CE) for the analysis of amino acids, and both normal and chiral separations are covered. In order to provide a general description of the main results and challenges in the biomedical field, some relevant applications and reviews on CE of amino acids are tabulated. Furthermore, some detailed experimental procedures are shown, regarding the CE analysis of amino acids in body fluids, in microdialysate, and released upon hydrolysis of proteins...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27627554/identification-of-small-peptides-of-acidic-collagen-extracts-from-silver-carp-skin-and-their-therapeutic-relevance
#14
Diana Wojtkowiak, Andrzej F Frydrychowski, Jakub Hadzik, Marzena Dominiak
BACKGROUND: Low-temperature techniques that prevent protein denaturation are being used to extract collagen from fish skin for cosmetic purposes. These extracts contain collagen with its triple helix structure preserved, as well as a number of other proteins. OBJECTIVES: The aim of the study was to investigate collagen extracts from the skin of silver carp for the presence of small-molecule peptides. MATERIAL AND METHODS: Liquid chromatography-mass spectrometry (HPLC-MS) was performed to analyze collagen extracts from silver carp skin for the presence of small-molecule peptides...
March 2016: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/27619930/kynurenine-pathway-and-white-matter-microstructure-in-bipolar-disorder
#15
Sara Poletti, Aye Mu Myint, Gregor Schüetze, Irene Bollettini, Elena Mazza, Doris Grillitsch, Clara Locatelli, Markus Schwarz, Cristina Colombo, Francesco Benedetti
Decreased availability of serotonin in the central nervous system has been suggested to be a central factor in the pathogenesis of depression. Activation of indoleamine 2-3 dioxygenase following a pro-inflammatory state could reduce the amount of tryptophan converted to serotonin and increase the production of tryptophan catabolites such as kynurenic acid, an antagonist of ionotropic excitatory aminoacid receptors, whose levels are reduced in bipolar disorder. Abnormalities in white matter (WM) integrity have been widely reported in BD...
September 12, 2016: European Archives of Psychiatry and Clinical Neuroscience
https://www.readbyqxmd.com/read/27607846/role-of-progranulin-in-the-regulation-of-vascular-tone-patho-physiological-implications
#16
J Bełtowski
No abstract text is available yet for this article.
September 8, 2016: Acta Physiologica
https://www.readbyqxmd.com/read/27603757/the-phosphatidylinositol-3-kinase-class-iii-complex-containing%C3%A2-tcvps15-and-tcvps34-participates-in-autophagy-in-trypanosoma-cruzi
#17
Alejandra C Schoijet, Tamara Sternlieb, Guillermo D Alonso
Autophagy is a degradative process by which eukaryotic cells digest their own components to provide aminoacids that may function as energy source under nutritional stress conditions. There is experimental evidence for autophagy in parasitic protists belonging to the family Trypanosomatidae. However, few proteins implicated in this process have been characterized so far in these parasites. Moreover, it has been shown that autophagy is involved in Trypanosoma cruzi differentiation and thus might have a role in pathogenicity...
September 7, 2016: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/27598177/design-and-synthesis-of-analogues-of-marine-natural-product-galaxamide-an-n-methylated-cyclic-pentapeptide-as-potential-anti-tumor-agent-in-vitro
#18
Jignesh Lunagariya, Shenghui Zhong, Jianwei Chen, Defa Bai, Poonam Bhadja, Weili Long, Xiaojian Liao, Xiaoli Tang, Shihai Xu
Herein, we report design and synthesis of novel 26 galaxamide analogues with N-methylated cyclo-pentapeptide, and their in vitro anti-tumor activity towards the panel of human tumor cell line, such as, A549, A549/DPP, HepG2 and SMMC-7721 using MTT assay. We have also investigated the effect of galaxamide and its representative analogues on growth, cell-cycle phases, and induction of apoptosis in SMMC-7721 cells in vitro. Reckon with the significance of conformational space and N-Me aminoacid (aa) comprising this compound template, we designed the analogues with modification in N-Me-aa position, change in aa configuration from l to d aa and substitute one Leu-aa to d/l Phe-aa residue with respective to the parent structure...
September 3, 2016: Marine Drugs
https://www.readbyqxmd.com/read/27598135/evaluation-of-anthelmintic-activity-and-composition-of-pumpkin-cucurbita-pepo-l-seed-extracts-in-vitro-and-in-vivo-studies
#19
Maciej Grzybek, Wirginia Kukula-Koch, Aneta Strachecka, Aleksandra Jaworska, Andrew M Phiri, Jerzy Paleolog, Krzysztof Tomczuk
UNLABELLED: A significant number of studies report growing resistance in nematodes thriving in both humans and livestock. This study was conducted to evaluate the in vitro and in vivo anthelmintic efficiency of Curcubita pepo (C. pepo) L. hot water extract (HWE), cold water extract (CWE) or ethanol extract (ETE) on two model nematodes: Caenorhabditis elegans (C. elegans) and Heligmosoides bakeri (H. bakeri). METHODS: Raman, IR and LC-MS spectroscopy analyses were performed on the studied plant material to deliver qualitative and quantitative data on the composition of the obtained extracts: ETE, HWE and CWE...
2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27593613/enhanced-phytostabilization-of-cadmium-by-a-halophyte-acanthus-ilicifolius-l
#20
A M Shackira, Jos T Puthur
Heavy metal pollution in mangrove wetlands has become a growing matter of concern as it serves as sink and source for toxic heavy metals including cadmium (Cd). The present study evaluates the phytostabilization potential of a halophyte, A. ilicifolius L. towards Cd under hydroponic culture conditions. Accumulation, translocation and effects of Cd on the anti-oxidant system of A. ilicifolius were studied. Results indicated that A. ilicifolius accumulated Cd mainly in roots (96.4%) as compared to stem (1.4%) and leaves (0...
September 3, 2016: International Journal of Phytoremediation
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