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biomarkers for tuberculosis

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https://www.readbyqxmd.com/read/28794177/discovery-and-validation-of-a-six-marker-serum-protein-signature-for-the-diagnosis-of-active-pulmonary-tuberculosis
#1
Mary A De Groote, David G Sterling, Thomas Hraha, Theresa Russell, Louis S Green, Kirsten Wall, Stephan Kraemer, Rachel Ostroff, Nebojsa Janjic, Urs A Ochsner
New non-sputum biomarker tests for active tuberculosis (TB) diagnostics are of highest priority for global TB control. We performed in-depth proteomic analysis using the 4000-plex SOMAscan assay on 1470 serum samples from seven TB-endemic countries. All samples were from patients with symptoms and signs suggestive of active pulmonary TB that were systematically confirmed or ruled-out for TB by culture and clinical follow-up. HIV-coinfection was present in 34%and 22% were sputum smear-negative. Serum protein biomarkers were identified by stability selection using L1-regularized logistic regression and by Kolmogorov-Smirnov (KS) statistics...
August 9, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28789704/immuno-diagnosis-of-mycobacterium-tuberculosis-in-sputum-and-reduction-of-timelines-for-its-positive-cultures-to-within-3%C3%A2-h-by-pathogen-specific-thymidylate-kinase-expression-assays
#2
Misaki Wayengera, Ivan Mwebaza, Johnson Welishe, Alice Bayiyana, David P Kateete, Eddie Wampande, Samuel Kirimunda, Edgar Kigozi, Fred Katabazi, Carol Musubika, Samuel Kyobe, Peace Babirye, Benon Asiimwe, Moses L Joloba
BACKGROUND: Laboratory diagnosis of Tuberculosis (TB) is traditionally based on microscopy and or culture. Microscopy is however, only sensitive to a specified degree of bacillary load not present in HIV co-infected persons. Traditional cultures of Mycobacterium tuberculosis (M. tb), on the other hand, take weeks to read-thereby delaying the critical decision whether or not, to treat. Although nucleic acids amplification tests (NAATS) applied directly on sputum or cultures can increase the sensitivity for TB diagnosis among those with HIV co-infection as well as reduce time-lines for positive culture detection, they do not replace the need for smear microscopy and culture...
August 8, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28782053/elucidating-the-structure-of-n-1-acetylisoputreanine-a-novel-polyamine-catabolite-in-human-urine
#3
Bryna L Fitzgerald, Sebabrata Mahapatra, Delphine K Farmer, Michael R McNeil, Robert A Casero, John T Belisle
An untargeted metabolomics approach was utilized to determine urinary metabolites that could serve as small-molecule biomarkers for treatment response to standard tuberculosis treatment. However, the majority of metabolites that most accurately distinguished patient samples at the time of diagnosis from those at 1 month after the start of therapy lacked structural identification. The detection of unknown metabolite structures is a well-known limitation of untargeted metabolomics and underscores a need for continued elucidation of novel metabolite structures...
July 31, 2017: ACS Omega
https://www.readbyqxmd.com/read/28766343/isothermal-point-mutation-detection-toward-a-first-pass-screening-strategy-for-multidrug-resistant-tuberculosis
#4
Benjamin Y C Ng, Eugene J H Wee, Kyra Woods, Will Anderson, Fiach Antaw, Hennes Z H Tsang, Nicholas P West, Matt Trau
Point mutations in DNA are useful biomarkers that can provide critical classification of disease for accurate diagnosis and to inform clinical decisions. Conventional approaches to detect point mutations are usually based on technologies such as real-time polymerase chain reaction (PCR) or DNA sequencing, which are typically slow and require expensive lab-based equipment. While rapid isothermal strategies such as recombinase polymerase amplification (RPA) have been proposed, they tend to suffer from poor specificity in discriminating point mutations...
August 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28756649/rnomic-identification-and-evaluation-of-npctb_6715-a-non-protein-coding-rna-gene-as-a-potential-biomarker-for-the-detection-of-mycobacterium-tuberculosis
#5
Priyatharisni Kanniappan, Siti Aminah Ahmed, Ganeswrie Rajasekaram, Citartan Marimuthu, Ewe Seng Ch'ng, Li Pin Lee, Carsten A Raabe, Timofey S Rozhdestvensky, Thean Hock Tang
Technological advances in RNA biology greatly improved transcriptome profiling during the last two decades. Besides the discovery of many small RNAs (sRNA) that are involved in the physiological and pathophysiological regulation of various cellular circuits, it becomes evident that the corresponding RNA genes might also serve as potential biomarkers to monitor the progression of disease and treatment. sRNA gene candidate npcTB_6715 was previously identified via experimental RNomic (unpublished data), and we report its application as potential biomarker for the detection of Mycobacterium tuberculosis (MTB) in patient samples...
July 29, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28752079/unique-chemokine-profiles-of-lung-tissues-distinguish-post-chemotherapeutic-persistent-and-chronic-tuberculosis-in-a-mouse-model
#6
Soomin Park, Seung-Hun Baek, Sang-Nae Cho, Young-Saeng Jang, Ahreum Kim, In-Hong Choi
There is a substantial need for biomarkers to distinguish latent stage from active Mycobacterium tuberculosis infections, for predicting disease progression. To induce the reactivation of tuberculosis, we present a new experimental animal model modified based on the previous model established by our group. In the new model, the reactivation of tuberculosis is induced without administration of immunosuppressive agents, which might disturb immune responses. To identify the immunological status of the persistent and chronic stages, we analyzed immunological genes in lung tissues from mice infected with M...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28743248/immunological-recovery-in-tuberculosis-hiv-co-infected-patients-on-antiretroviral-therapy-implication-for-tuberculosis-preventive-therapy
#7
Basel Karo, Gérard Krause, Stefanie Castell, Christian Kollan, Osamah Hamouda, Walter Haas
BACKGROUND: Understanding the immune response to combination antiretroviral therapy (cART) is essential for a clear approach to tuberculosis (TB) preventive therapy. We investigated the immunological recovery in cART-treated HIV-infected patients developing TB compared to those who remained free of TB. METHODS: We extracted data of HIV-infected patients from a multicenter cohort for the HIV clinical surveillance in Germany. No patients included in our study had TB at the beginning of the observation...
July 25, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28709490/serum-levels-of-soluble-urokinase-plasminogen-activator-receptor-supar-as-a-marker-of-tuberculosis-treatment-efficacy
#8
V Indumati, V Vijay, D Krishnaswamy, V Rajeshwari, A Ramesh, D Shantala, A Shilpa
BACKGROUND: Upon diagnosis, Pulmonary Tuberculosis patients are treated for TB for a period of 6-9 months. At present, there exists very little indication of the efficacy of the particular treatment. A few previous studies have shown that soluble urokinase plasminogen activator receptor (SuPAR) may be used as treatment efficacy marker. SuPAR is a cellular receptor for serine protease urokinase plasminogen activator (uPA). Bacterial endotoxins and cytokines of the innate immune system stimulate the secretion of uPA in monocytes & neutrophils...
July 2017: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/28701467/the-cross-species-mycobacterial-growth-inhibition-assay-mgia-project-2010-2014
#9
Michael J Brennan, Rachel Tanner, Sheldon Morris, Thomas J Scriba, Jacqueline M Achkar, Andrea Zelmer, David Hokey, Angelo Izzo, Sally Sharpe, Ann Williams, Adam Penn-Nicholson, Mzwandile Erasmus, Elena Stylianou, Daniel F Hoft, Helen McShane, Helen A Fletcher
The development of a functional biomarker assay in the tuberculosis (TB) field would be widely recognized as a major advance in efforts to efficiently develop and test novel TB vaccine candidates. We present preliminary studies using mycobacterial growth inhibition assays (MGIAs) to detect a BCG vaccine response across species, and extend this work to determine if a standardized MGIA can be applied in characterizing new generation TB vaccines. The comparative MGIA studies reviewed here aimed to evaluate robustness, reproducibility, and ability to reflect in vivo responses...
July 12, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28698665/antigens-rv0310c-and-rv1255c-are-promising-novel-biomarkers-for-the-diagnosis-of-mycobacterium-tuberculosis-infection
#10
Liulin Luo, Lin Zhu, Jun Yue, Jianping Liu, Guoyuan Liu, Xuelian Zhang, Honghai Wang, Ying Xu
This study aimed to identify novel immunogenic epitopes from Mycobacterium tuberculosis (MTB) that could be used in tuberculosis (TB) diagnostics. To determine the diagnostic potential of mycobacterial antigens in serodiagnosis of TB, 256 patients were enrolled in a study and divided into two groups: 126 smear-positive pulmonary TB patients (SPPT) and 130 smear-negative pulmonary TB patients (SNPT); 152 bacillus Calmette-Guerin (BCG)-vaccinated healthy people were used as a control. Murine results showed that antigens Rv0310c-E from RD 8 and Rv1255c-E from RD 10 were strongly immunogenic to Th1 cells and induced a great humoral response...
July 12, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28689652/field-evaluation-of-candidate-baits-for-oral-delivery-of-bcg-vaccine-to-european-badgers-meles-meles
#11
Kate Palphramand, Richard Delahay, Andrew Robertson, Sonya Gowtage, Gareth A Williams, Robbie A McDonald, Mark Chambers, Stephen P Carter
The control of tuberculosis (TB) in cattle in the UK and Ireland is compromised by transmission of Mycobacterium bovis to cattle from the European badger (Meles meles), which acts as a wildlife reservoir. Vaccination of badgers could potentially contribute to TB control but the only licensed vaccine is injectable BadgerBCG which requires the live-capture of badgers. Current research is aimed at developing an oral TB vaccine (where vaccine is contained within bait) that is intended to be more cost-effective to deploy over large areas...
July 6, 2017: Vaccine
https://www.readbyqxmd.com/read/28683213/proteomics-of-culture-filtrate-of-prevalent-mycobacterium-tuberculosis-strains-2d-page-map-and-maldi-tof-ms-analysis
#12
Gavish Kumar, Hari Shankar, Divakar Sharma, Prashant Sharma, Deepa Bisht, Vishwa M Katoch, Beenu Joshi
Although diverse efforts have been done to identify biomarkers for control of tuberculosis using laboratory strain Mycobacterium tuberculosis H37Rv, the disease still poses a threat to mankind. There are many emerging M. tuberculosis strains, and proteomic profiling of these strains might be important to find out potential targets for diagnosis and/or prevention of tuberculosis. We evaluated the comparative proteomic profiling of culture filtrate (CF) proteins from prevalent M. tuberculosis strains (Central Asian or Delhi type; CAS1_Del, East African-Indian; EAI-3 and Beijing family) by 2D polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry...
July 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28681821/risk-factors-and-frequency-of-tuberculosis-associated-immune-reconstitution-inflammatory-syndrome-among-hiv-tuberculosis-co-infected-patients-in-southern-india
#13
Ramachandran Vignesh, Chinnambedu R Swathirajan, Sunil S Solomon, Esaki Muthu Shankar, Kailapuri G Murugavel
Immune reconstitution inflammatory syndrome (IRIS) continues to be a complication in HIV/tuberculosis (TB) co-infected patients initiating highly active antiretroviral therapy (HAART). The aim of this study was to evaluate the risk factors associated with developing IRIS to identify a possible biomarker to predict or diagnose IRIS in patients initiating HAART. A total of 175 HIV/TB co-infected patients initiating HAART were followed up longitudinally during September 2010 to May 2013 attending a HIV care clinic in Chennai...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28655597/identification-of-host-proteins-predictive-of-early-stage-mycobacterium-tuberculosis-infection
#14
Charles M Bark, Ameur M Manceur, LaShaunda L Malone, Mary Nsereko, Brenda Okware, Harriet K Mayanja, Moses L Joloba, Isabelle Rajotte, Marija Mentinova, Phyla Kay, Seydina Lo, Patrick Tremblay, Catherine M Stein, W Henry Boom, Eustache Paramithiotis
The objective of this study was to identify blood-based protein biomarkers of early stage Mycobacterium tuberculosis (Mtb) infection. We utilized plasma and serum specimens from TB patients and their contacts (age≥12) enrolled in a household contact study in Uganda. In the discovery phase cross-sectional samples from 104 HIV-uninfected persons classified as either active TB, latent Mtb infection (LTBI), tuberculin skin test (TST) converters, or persistent TST-negative were analyzed. Two hundred eighty-nine statistically significant (false discovery rate corrected p<0...
July 2017: EBioMedicine
https://www.readbyqxmd.com/read/28634479/whole-blood-profiling-of-bacillus-calmette-gu%C3%A3-rin-induced-trained-innate-immunity-in-infants-identifies-epidermal-growth-factor-il-6-platelet-derived-growth-factor-ab-bb-and-natural-killer-cell-activation
#15
Steven G Smith, Johanneke Kleinnijenhuis, Mihai G Netea, Hazel M Dockrell
Vaccination of infants with bacillus Calmette-Guérin (BCG) activates both the innate and adaptive arms of the immune response. The antimycobacterial effects of these responses most likely account for the ability of BCG to protect against childhood forms of tuberculosis (TB). There is also evidence for a heterologous protective effect of BCG vaccination against TB-unrelated mortality in low birth weight infants. A possible mechanism of action of this effect, the induction of trained innate immunity, has been demonstrated when cells from BCG-vaccinated adults are restimulated in vitro with non-related microbial stimuli...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28628015/latent-tuberculosis-potential-biomarkers-reactivation-of-infection
#16
I Pantsulaia, N Kikodze, T Chikovani
Tuberculosis continues to be one of the most important infectious causes of death worldwide. Despite substantial investments and progress made in expansion of directly observed therapy short course (DOTS) strategy, improved treatment completion rates and inadequate case detection remains a major obstacle for global control of Tuberculosis. The global case detection rate has increased from 28% to 60%. However, it is estimated that approximately 50% of patients with tuberculosis are still not diagnosed or treated appropriately...
May 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28627685/metabolomic-analysis-based-on-1h-nuclear-magnetic-resonance-spectroscopy-metabolic-profiles-in-tuberculous-malignant-and-transudative-pleural-effusion
#17
Cheng Wang, Jingjin Peng, Yanling Kuang, Jiaqiang Zhang, Luming Dai
Pleural effusion is a common clinical manifestation with various causes. Current diagnostic and therapeutic methods have exhibited numerous limitations. By involving the analysis of dynamic changes in low molecular weight catabolites, metabolomics has been widely applied in various types of disease and have provided platforms to distinguish many novel biomarkers. However, to the best of our knowledge, there are few studies regarding the metabolic profiling for pleural effusion. In the current study, 58 pleural effusion samples were collected, among which 20 were malignant pleural effusions, 20 were tuberculous pleural effusions and 18 were transudative pleural effusions...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28610788/evaluation-of-antigen-specific-interleukin-1%C3%AE-as-a-biomarker-to-detect-cattle-infected-with-mycobacterium-bovis
#18
Mahmoud M Elnaggar, Gaber S Abdellrazeq, Alaa Elsisy, Asmaa H Mahmoud, Abdelrazeq Shyboub, Martina Sester, Samy A Khaliel, Mahavir Singh, Helmy A Torky, William C Davis
Bovine tuberculosis (bTB) is a major world-wide health problem that has been difficult to control, due to the lack of an effective vaccine and limited ability of the tuberculin skin test (TST) and the ancillary whole blood interferon-gamma (IFN-γ) release assay (IGRA) to detect all infected animals. A 6 h cytokine flow cytometric IFN-γ (CFC) assay was developed in effort to overcome these limitations and expand methods for studying the mechanisms of bTB immunopathogenesis. The present study was conducted to evaluate IL-1β as a biomarker to use in conjunction with the IFN-γ CFC assay to improve the diagnostic accuracy for bTB...
July 2017: Tuberculosis
https://www.readbyqxmd.com/read/28608729/accelerating-tuberculosis-vaccine-trials-with-diagnostic-and-prognostic-biomarkers
#19
Stefan H E Kaufmann, January Weiner, Jeroen Maertzdorf
The most recent estimates on tuberculosis (TB) morbidity and mortality reveal that the global disease burden is even higher than previously assumed. Better drugs, diagnostics and vaccines are major requirements to control the ongoing TB pandemic. The high complexity of the infectious process and the underlying pathology, however, challenge elucidation of protective immune mechanisms at the various stages towards active TB disease, which need to be understood for rational design of novel intervention measures...
August 2017: Expert Review of Vaccines
https://www.readbyqxmd.com/read/28605426/biomarkers-of-cerebral-injury-and-inflammation-in-pediatric-tuberculous-meningitis
#20
Ursula K Rohlwink, Katya Mauff, Katalin A Wilkinson, Nico Enslin, Emmanuel Wegoye, Robert J Wilkinson, Anthony A Figaji
Background: Tuberculous meningitis (TBM) leads to death or disability in half the affected individuals. Tools to assess severity and predict outcome are lacking. Neuro-specific biomarkers could serve as markers of the severity and evolution of brain injury, but have not been widely explored in TBM. We examined biomarkers of neurological injury (neuromarkers) and inflammation in pediatric TBM and their association with outcome. Methods: Blood and cerebrospinal fluid (CSF) of children with TBM and hydrocephalus taken on admission and over 3 weeks were analysed for neuromarkers S100B, neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP), and multiple inflammatory markers...
June 9, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
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