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biomarkers for tuberculosis

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https://www.readbyqxmd.com/read/28928738/age-stratified-t-cell-responses-in-children-infected-with-mycobacterium-tuberculosis
#1
Alexandra Dreesman, Véronique Corbière, Violette Dirix, Kaat Smits, Sara Debulpaep, Iris De Schutter, Myriam Libin, Mahavir Singh, Anne Malfroot, Camille Locht, Françoise Mascart
Tuberculosis (TB) in young children differs from adult TB in that the risk of rapid progression to active TB (aTB) is higher in children than in adults. The reasons for this increased risk are not fully understood. Early differentiation remains difficult between children at risk to develop aTB from those who will remain healthy and develop a latent TB infection (LTBI). Biomarkers to differentiate aTB from LTBI in children, especially in very young children, are urgently needed. To identify M. tuberculosis-specific functional T cell subsets related to clinical manifestations in children, we enrolled 87 children exposed to M...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28910318/characterization-of-a-novel-panel-of-plasma-micrornas-that-discriminates-between-mycobacterium-tuberculosis-infection-and-healthy-individuals
#2
Jia-Yi Cui, Hong-Wei Liang, Xin-Ling Pan, Di Li, Na Jiao, Yan-Hong Liu, Jin Fu, Xiao-Yu He, Gao-Xiang Sun, Chun-Lei Zhang, Chi-Hao Zhao, Dong-Hai Li, En-Yu Dai, Ke Zen, Feng-Min Zhang, Chen-Yu Zhang, Xi Chen, Hong Ling
Cavities are important in clinical diagnosis of pulmonary tuberculosis (TB) infected by Mycobacterium tuberculosis. Although microRNAs (miRNAs) play a vital role in the regulation of inflammation, the relation between plasma miRNA and pulmonary tuberculosis with cavity remains unknown. In this study, plasma samples were derived from 89 cavitary pulmonary tuberculosis (CP-TB) patients, 89 non-cavitary pulmonary tuberculosis (NCP-TB) patients and 95 healthy controls. Groups were matched for age and gender. In the screening phase, Illumina high-throughput sequencing technology was employed to analyze miRNA profiles in plasma samples pooled from CP-TB patients, NCP-TB patients and healthy controls...
2017: PloS One
https://www.readbyqxmd.com/read/28905401/mycothiol-acetyltransferase-rv0819-of-mycobacterium-tuberculosis-is-a-potential-biomarker-for-direct-diagnosis-of-tuberculosis-using-patient-serum-specimens
#3
H Zeitoun, M Bahey-El-Din, M A Kassem, H M Aboushleib
Mycobacterium tuberculosis infection constitutes a global threat that results in significant morbidity and mortality worldwide. Efficient and early diagnosis of tuberculosis (TB) is of paramount importance for successful treatment. The aim of the current study is to investigate the mycobacterial mycothiol acetyltransferase Rv0819 as a potential novel biomarker for diagnosis of active TB infection. The gene encoding Rv0819 was cloned and successfully expressed in Escherichia coli. The recombinant Rv0819 was purified using metal affinity chromatography and was used to raise murine polyclonal antibodies against Rv0819...
September 14, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28889065/profiling-dendritic-cell-subsets-in-the-patients-with-active-pulmonary-tuberculosis
#4
Yuan-Bin Lu, De-Qian Xiao, Kui-Di Liang, Jun-Ai Zhang, Wan-Dang Wang, Shi-Yan Yu, Bi-Ying Zheng, Yu-Chi Gao, You-Chao Dai, Yan Jia, Chen Chen, Ze-Gang Zhuang, Xin Wang, Xiao-Xia Fu, Yong Zhou, Jixin Zhong, Zheng W Chen, Jun-Fa Xu
Dendritic cell (DC) plays an important role in the immune response against pulmonary tuberculosis. However, the phenotypic profile of DC subsets in peripheral blood in individuals with active pulmonary tuberculosis (APT) is still inconclusive. Here, we demonstrated that the absolute numbers of total DC (tDC), myeloid DC (mDC) and plasmacytoid DC (pDC) in individuals with APT were decreased compared to healthy controls (HCs). The decreased number of DCs, especially of pDC, seems to be a useful diagnostic marker of APT...
September 7, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28881572/a-single-nucleotide-polymorphism-in-the-interferon-%C3%AE-gene-ifng-874-t-a-is-associated-with-susceptibility-to-tuberculosis
#5
Zhang Wei, Shen Wenhao, Mi Yuanyuan, Li Yang, Zhou Daming, Xian Jiangchun, Jiang Jijun
Interferon-γ (Interferon gamma, IFNG) is an important cytokine involved in providing resistance to mycobacterial diseases. Common variants of IFNG, such as IFNG +874 T/A(rs2430561), may be related to tuberculosis susceptibility, but this association has not been consistently observed. We performed an updated meta-analysis to evaluate the association between the IFNG +874 T/A (rs2430561) polymorphism and tuberculosis susceptibility. PubMed and SinoMed databases were searched up to October 2016, and odds ratios (OR) and 95% confidence intervals (CI) were used to assess the association strength...
August 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28881482/altered-expression-of-antigen-specific-memory-and-regulatory-t-cell-subsets-differentiate-latent-and-active-tuberculosis
#6
Balaji Pathakumari, Santhi Devasundaram, Alamelu Raja
Though one-third of the world population is infected with M. tuberculosis, only 5-10% of the infected individuals will develop active TB disease and rest will remain infected with no symptoms known as latent TB infection (LTBI). Identifying biomarkers that differentiate latent and active TB disease enables effective TB control, since early detection, treatment of active TB and preventive treatment of LTBI individuals are crucial steps involved in TB control. Here, we have evaluated the frequency of antigen specific memory and regulatory T cells (Tregs) in 15 healthy household contacts (HHC) and 15 pulmonary TB patients (PTB) to identify biomarkers for differential diagnosis of LTBI and active TB...
September 7, 2017: Immunology
https://www.readbyqxmd.com/read/28864369/predictive-factors-and-treatment-outcomes-of-tuberculous-pleural-effusion-in-patients-with-cancer-and-pleural-effusion
#7
Jaehee Lee, Yong Dae Lee, Jae Kwang Lim, Deok Heon Lee, Seung Soo Yoo, Shin Yup Lee, Seung Ick Cha, Jae Yong Park, Chang Ho Kim
BACKGROUND: Patients with cancer are at an increased risk of tuberculosis. As pleural effusion has great clinical significance in patients with cancer, the differential diagnosis between tuberculous pleural effusion (TPE) and malignant pleural effusion (MPE) is important. However, the predictive factors and treatment outcomes of TPE in patients with cancer have rarely been studied. MATERIALS AND METHODS: Confirmed TPE cases identified at cancer diagnosis and during anticancer management from 2008-2015 were retrospectively investigated...
August 2017: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/28859607/suitability-of-saliva-for-tuberculosis-diagnosis-comparing-with-serum
#8
Anna Ritah Namuganga, Novel N Chegou, Paul Mubiri, Gerhard Walzl, Harriet Mayanja-Kizza
BACKGROUND: In the search for fast, simple and better ways for diagnosis of tuberculosis (TB), there is need to discover and evaluate new biomarkers that are found in samples other than sputum to determine their effectiveness. This study examined the utility of saliva vis-a-vis serum by evaluating levels of biomarkers found in saliva and serum from TB suspects. METHODS: Study enrolled tuberculosis suspects. Sputum MGIT was used as the gold standard for active TB...
August 31, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28854691/the-clinical-recovery-of-tuberculosis-patients-undergoing-specific-treatment-is-associated-with-changes-in-the-immune-and-neuroendocrine-responses
#9
Ariana Díaz, Bettina Bongiovanni, Luciano D'Attilio, Natalia Santucci, Griselda Dídoli, Rocío Del Valle Fernández, Leandro Kovalevski, Susana Lioi, Walter Gardeñez, Nadia Brandan, Luis J Nannini, Hugo Besedovsky, Adriana Del Rey, Oscar Bottasso, María Luisa Bay
Tuberculosis (TB) caused by Mycobacterium tuberculosis is a health problem worldwide. Patients with pulmonary TB show a neuro-immune-endocrine imbalance characterized by an impaired cellular immunity together with increased plasma levels of cortisol, pro- and anti-inflammatory cytokines and markedly decreased dehydroepiandrosterone (DHEA) levels. Extending these findings, we now investigated the immune-endocrine profile of TB patients undergoing specific treatment. Patients (n = 24) were bled at diagnosis (T0), 2, 4, 6 months after treatment initiation and 3 months following its completion...
September 29, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28846924/the-circular-rna-of-peripheral-blood-mononuclear-cells-hsa_circ_0005836-as-a-new-diagnostic-biomarker-and-therapeutic-target-of-active-pulmonary-tuberculosis
#10
Ze-Gang Zhuang, Jun-Ai Zhang, Hou-Long Luo, Gan-Bin Liu, Yuan-Bin Lu, Nan-Hai Ge, Bi-Ying Zheng, Rui Xi Li, Chen Chen, Xin Wang, Yu-Qing Liu, Feng-Hui Liu, Yong Zhou, Xiao-Zhen Cai, Zheng W Chen, Jun-Fa Xu
It has been reported that circular RNA (circRNA) is associated with human cancer. However, few studies have been reported in active pulmonary tuberculosis (APTB). The global circRNA expression was detected in the peripheral blood mononuclear cells (PBMCs) of APTB patients (n=5) and health controls (HC) (n=5) by using high-throughput sequencing. According to the systematical bioinformatics analysis, the basic content of circRNAs and their fold changes in the two groups were calculated. We selected 6 significant differentially expressed circRNAs, hsa_circ_0005836, hsa_circ_0009128, hsa_circ_0003519, hsa_circ_0023956, hsa_circ_0078768, and hsa_circ_0088452 and validated the expression in PBMCs from APTB (n=10) and HC (n=10) by real-time quantitative reverse transcription-polymerase chain reactions (qRT-PCRs)...
October 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28841837/decreased-ex-vivo-production-of-interferon-gamma-is-associated-with-severity-and-poor-prognosis-in-patients-with-lupus
#11
Sung Soo Ahn, Eun Seong Park, Joo Sung Shim, Sang-Jun Ha, Beom Seok Kim, Seung Min Jung, Sang-Won Lee, Yong-Beom Park, Jason Jungsik Song
BACKGROUND: Lupus pathogenesis is closely associated with interferon gamma (IFN-γ), which plays a central role in innate and adaptive immunity. The aim of this study was to evaluate the ex vivo production of IFN-γ after stimulation of peripheral blood mononuclear cells with phytohemagglutinin (PHA) in patients with lupus, according to disease activity. METHODS: This study included 118 patients with lupus who had undergone IFN-γ-releasing assays (IGRAs) to screen for tuberculosis...
August 25, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28820984/identification-of-m-tuberculosis-antigens-in-the-sera-of-tuberculosis-patients-using-biomimetic-affinity-chromatography-in-conjunction-with-esi-cid-ms-ms
#12
Guorong Ma, Jinting Pan, Jincheng Han, Lina Gao, Shulin Zhang, Rongxiu Li
The profiling of abnormally-expressed proteins in host cells using mass spectrometry (MS) analysis is a classical approach for screening disease-associated biomarkers in clinical diagnosis. However, few pathogen-specific antigens can currently be detected in serum using this proteomic approach, since these are very low-abundant proteins that are easily masked by host high-abundant proteins. Identification of pathogen-specific antigens in the sera of tuberculosis patients is crucial for the clinical diagnosis of this infectious disease, especially in immune-compromised patients...
September 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28794177/discovery-and-validation-of-a-six-marker-serum-protein-signature-for-the-diagnosis-of-active-pulmonary-tuberculosis
#13
Mary A De Groote, David G Sterling, Thomas Hraha, Theresa Russell, Louis S Green, Kirsten Wall, Stephan Kraemer, Rachel Ostroff, Nebojsa Janjic, Urs A Ochsner
New non-sputum biomarker tests for active tuberculosis (TB) diagnostics are of highest priority for global TB control. We performed in-depth proteomic analysis using the 4000-plex SOMAscan assay on 1470 serum samples from seven TB-endemic countries. All samples were from patients with symptoms and signs suggestive of active pulmonary TB that were systematically confirmed or ruled-out for TB by culture and clinical follow-up. HIV-coinfection was present in 34%and 22% were sputum smear-negative. Serum protein biomarkers were identified by stability selection using L1-regularized logistic regression and by Kolmogorov-Smirnov (KS) statistics...
August 9, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28789704/immuno-diagnosis-of-mycobacterium-tuberculosis-in-sputum-and-reduction-of-timelines-for-its-positive-cultures-to-within-3%C3%A2-h-by-pathogen-specific-thymidylate-kinase-expression-assays
#14
Misaki Wayengera, Ivan Mwebaza, Johnson Welishe, Alice Bayiyana, David P Kateete, Eddie Wampande, Samuel Kirimunda, Edgar Kigozi, Fred Katabazi, Carol Musubika, Samuel Kyobe, Peace Babirye, Benon Asiimwe, Moses L Joloba
BACKGROUND: Laboratory diagnosis of Tuberculosis (TB) is traditionally based on microscopy and or culture. Microscopy is however, only sensitive to a specified degree of bacillary load not present in HIV co-infected persons. Traditional cultures of Mycobacterium tuberculosis (M. tb), on the other hand, take weeks to read-thereby delaying the critical decision whether or not, to treat. Although nucleic acids amplification tests (NAATS) applied directly on sputum or cultures can increase the sensitivity for TB diagnosis among those with HIV co-infection as well as reduce time-lines for positive culture detection, they do not replace the need for smear microscopy and culture...
August 8, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28782053/elucidating-the-structure-of-n-1-acetylisoputreanine-a-novel-polyamine-catabolite-in-human-urine
#15
Bryna L Fitzgerald, Sebabrata Mahapatra, Delphine K Farmer, Michael R McNeil, Robert A Casero, John T Belisle
An untargeted metabolomics approach was utilized to determine urinary metabolites that could serve as small-molecule biomarkers for treatment response to standard tuberculosis treatment. However, the majority of metabolites that most accurately distinguished patient samples at the time of diagnosis from those at 1 month after the start of therapy lacked structural identification. The detection of unknown metabolite structures is a well-known limitation of untargeted metabolomics and underscores a need for continued elucidation of novel metabolite structures...
July 31, 2017: ACS Omega
https://www.readbyqxmd.com/read/28766343/isothermal-point-mutation-detection-toward-a-first-pass-screening-strategy-for-multidrug-resistant-tuberculosis
#16
Benjamin Y C Ng, Eugene J H Wee, Kyra Woods, Will Anderson, Fiach Antaw, Hennes Z H Tsang, Nicholas P West, Matt Trau
Point mutations in DNA are useful biomarkers that can provide critical classification of disease for accurate diagnosis and to inform clinical decisions. Conventional approaches to detect point mutations are usually based on technologies such as real-time polymerase chain reaction (PCR) or DNA sequencing, which are typically slow and require expensive lab-based equipment. While rapid isothermal strategies such as recombinase polymerase amplification (RPA) have been proposed, they tend to suffer from poor specificity in discriminating point mutations...
September 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28756649/rnomic-identification-and-evaluation-of-npctb_6715-a-non-protein-coding-rna-gene-as-a-potential-biomarker-for-the-detection-of-mycobacterium-tuberculosis
#17
Priyatharisni Kanniappan, Siti Aminah Ahmed, Ganeswrie Rajasekaram, Citartan Marimuthu, Ewe Seng Ch'ng, Li Pin Lee, Carsten A Raabe, Timofey S Rozhdestvensky, Thean Hock Tang
Technological advances in RNA biology greatly improved transcriptome profiling during the last two decades. Besides the discovery of many small RNAs (sRNA) that are involved in the physiological and pathophysiological regulation of various cellular circuits, it becomes evident that the corresponding RNA genes might also serve as potential biomarkers to monitor the progression of disease and treatment. sRNA gene candidate npcTB_6715 was previously identified via experimental RNomic (unpublished data), and we report its application as potential biomarker for the detection of Mycobacterium tuberculosis (MTB) in patient samples...
July 29, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28752079/unique-chemokine-profiles-of-lung-tissues-distinguish-post-chemotherapeutic-persistent-and-chronic-tuberculosis-in-a-mouse-model
#18
Soomin Park, Seung-Hun Baek, Sang-Nae Cho, Young-Saeng Jang, Ahreum Kim, In-Hong Choi
There is a substantial need for biomarkers to distinguish latent stage from active Mycobacterium tuberculosis infections, for predicting disease progression. To induce the reactivation of tuberculosis, we present a new experimental animal model modified based on the previous model established by our group. In the new model, the reactivation of tuberculosis is induced without administration of immunosuppressive agents, which might disturb immune responses. To identify the immunological status of the persistent and chronic stages, we analyzed immunological genes in lung tissues from mice infected with M...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28743248/immunological-recovery-in-tuberculosis-hiv-co-infected-patients-on-antiretroviral-therapy-implication-for-tuberculosis-preventive-therapy
#19
Basel Karo, Gérard Krause, Stefanie Castell, Christian Kollan, Osamah Hamouda, Walter Haas
BACKGROUND: Understanding the immune response to combination antiretroviral therapy (cART) is essential for a clear approach to tuberculosis (TB) preventive therapy. We investigated the immunological recovery in cART-treated HIV-infected patients developing TB compared to those who remained free of TB. METHODS: We extracted data of HIV-infected patients from a multicenter cohort for the HIV clinical surveillance in Germany. No patients included in our study had TB at the beginning of the observation...
July 25, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28709490/serum-levels-of-soluble-urokinase-plasminogen-activator-receptor-supar-as-a-marker-of-tuberculosis-treatment-efficacy
#20
V Indumati, V Vijay, D Krishnaswamy, V Rajeshwari, A Ramesh, D Shantala, A Shilpa
BACKGROUND: Upon diagnosis, Pulmonary Tuberculosis patients are treated for TB for a period of 6-9 months. At present, there exists very little indication of the efficacy of the particular treatment. A few previous studies have shown that soluble urokinase plasminogen activator receptor (SuPAR) may be used as treatment efficacy marker. SuPAR is a cellular receptor for serine protease urokinase plasminogen activator (uPA). Bacterial endotoxins and cytokines of the innate immune system stimulate the secretion of uPA in monocytes & neutrophils...
July 2017: Indian Journal of Tuberculosis
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