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https://www.readbyqxmd.com/read/29311364/isg15-induced-il-10-is-a-novel-anti-inflammatory-myeloid-axis-disrupted-during-active-tuberculosis
#1
Paula Fernandes Dos Santos, Johan Van Weyenbergh, Murilo Delgobo, Daniel de Oliveira Patricio, Brian J Ferguson, Rodrigo Guabiraba, Tim Dierckx, Soraya Maria Menezes, André Báfica, Daniel Santos Mansur
IFN-stimulated gene 15 (ISG15) deficiency in humans leads to severe IFNopathies and mycobacterial disease, the latter being previously attributed to its extracellular cytokine-like activity. In this study, we demonstrate a novel role for secreted ISG15 as an IL-10 inducer, unique to primary human monocytes. A balanced ISG15-induced monocyte/IL-10 versus lymphoid/IFN-γ expression, correlating with p38 MAPK and PI3K signaling, was found using targeted in vitro and ex vivo systems analysis of human transcriptomic datasets...
January 8, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29311240/potential-immunological-biomarkers-for-detection-of-mycobacterium-tuberculosis-infection-in-endemic-setting-ethiopia
#2
Takele Teklu, Kwon Keehwan, Biniam Wondale, Milkessa HaileMariam, Mengistu Legesse, Girmay Medhin, Aboma Zewude, Rembert Pieper, Gobena Ameni
Accurate diagnosis and early treatment of tuberculosis (TB) and latent TB infection (LTBI) are vital to prevent and control TB. The lack of specific biomarkers hinders these efforts. This study's purpose was to screen immunological markers that discriminate M. tuberculosis (Mtb) infection outcomes in an endemic setting of Ethiopia. Whole blood from 90 participants was stimulated using the ESAT-6/CFP-10 antigen cocktail. The interferon-γ (IFN-γ)-based QuantiFERON diagnostic test was used to distinguish between LTBI and uninfected control cases...
January 8, 2018: Infection and Immunity
https://www.readbyqxmd.com/read/29310595/potential-of-dosr-and-rpf-antigens-from-mycobacterium-tuberculosis-to-discriminate-between-latent-and-active-tuberculosis-in-a-tuberculosis-endemic-population-of-medellin-colombia
#3
Leonar Arroyo, Diana Marín, Kees L M C Franken, Tom H M Ottenhoff, Luis F Barrera
BACKGROUND: Tuberculosis (TB) remains one of the most deadly infectious diseases. One-third to one-fourth of the human population is estimated to be infected with Mycobacterium tuberculosis (Mtb) without showing clinical symptoms, a condition called latent TB infection (LTBI). Diagnosis of Mtb infection is based on the immune response to a mixture of mycobacterial antigens (PPD) or to Mtb specific ESAT-6/CFP10 antigens (IGRA), highly expressed during the initial phase of infection. However, the immune response to PPD and IGRA antigens has a low power to discriminate between LTBI and PTB...
January 8, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29306028/unique-mtb-protein-found-in-urine-of-tb-patients-discovery-of-a-unique-mycobacterium-tuberculosis-protein-through-proteomic-analysis-of-urine-from-patients-with-active-tuberculosis
#4
Nira Pollock, Rakesh Dhiman, Nada Daifalla, Maha Farhat, Antonio Campos-Neto
Identification of pathogen-specific biomarkers present in patients' serum or urine samples can be a useful diagnostic approach. In efforts to discover Mycobacterium tuberculosis (Mtb) biomarkers we identified by mass spectroscopy a unique 21-mer Mtb peptide sequence (VVLGLTVPGGVELLPGVALPR) present in the urines of TB patients from Zimbabwe. This peptide has 100% sequence homology with the protein TBCG_03312 from the C strain of Mtb (a clinical isolate identified in New York, NY, USA) and 95% sequence homology with Mtb oxidoreductase (MRGA423_21210) from the clinical isolate MTB423 (identified in Kerala, India)...
January 3, 2018: Microbes and Infection
https://www.readbyqxmd.com/read/29289622/presepsin-as-a-novel-diagnostic-biomarker-for-differentiating-active-pulmonary-tuberculosis-from-bacterial-community-acquired-pneumonia
#5
Zhi-Jiang Qi, Han Yu, Jing Zhang, Chun-Sheng Li
BACKGROUND: The expression of presepsin in active pulmonary tuberculosis (APTB) is unknown. We observed the expression of presepsin in APTB, and to evaluate the value for discriminating between APTB and bacterial community acquired pneumonia (BCAP). METHODS: Consecutive APTB patients who were accurately diagnosed by sputum culture and BCAP patients were enrolled from August 2013 to July 2015. Clinical data were collected, and plasma presepsin concentrations were tested...
December 28, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29287920/microbiological-diagnosis-of-infections-caused-by-the-genus-mycobacterium
#6
Sofía Samper, Julià González-Martin
Mycobacterial culture has a high sensitivity and is the test of choice for the microbiological diagnosis of tuberculosis and nontuberculous mycobacterial infections. However, the results of this culture require at least 2-3 weeks to obtain positivity. Staining is rapid and can be used as a complementary study, although its sensitivity is low. Gene amplification tests have an intermediate sensitivity and obtain results in 1-2 days. These last tests are indicated in cases with moderate or high clinical suspicion...
December 26, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29281987/orfeome-based-identification-of-biomarkers-for-serodiagnosis-of-mycobacterium-tuberculosis-latent-infection
#7
Fangbin Zhou, Xindong Xu, Sijia Wu, Xiaobing Cui, Weiqing Pan
BACKGROUND: The challenges posed by Mycobacterium tuberculosis infection require the gradual removal of the pool of latent tuberculosis infection (LTBI). The current cell-immune-based diagnostic tests used to identify LTBI individuals have several irreversible drawbacks. In the present study, we attempted to identify novel diagnostic antigens for LTBI. METHODS: A high-throughput glutathione S-transferase (GST)-fusion technology was used to express over 409 TB proteins and sera from LTBI and healthy individuals was used to interrogate these GST-TB fusion proteins...
December 28, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29260600/recent-insights-into-human-bronchial-proteomics-how-are-we-progressing-and-what-is-next
#8
Heng Wee Tan, Yan-Ming Xu, Dan-Dan Wu, Andy T Y Lau
The human respiratory system is highly prone to diseases and complications. Many lung diseases, including lung cancer (LC), tuberculosis (TB), and chronic obstructive pulmonary disease (COPD) have been among the most common causes of death worldwide. Cystic fibrosis (CF), the most common genetic disease in Caucasians, has adverse impacts on the lungs. Bronchial proteomics plays a significant role in understanding the underlying mechanisms and pathogenicity of lung diseases and provides insights for biomarker and therapeutic target discoveries...
December 20, 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/29250493/determination-of-lipoprotein-z-specific-iga-in-tuberculosis-and-latent-tuberculosis-infection
#9
Jia-Ni Xiao, Yanqing Xiong, Yingying Chen, Yang-Jiong Xiao, Ping Ji, Yong Li, Shu-Jun Wang, Guo-Ping Zhao, Qi-Jian Cheng, Shui-Hua Lu, Ying Wang
Tuberculosis (TB) remains one of the most severe infectious diseases. It is still of paramount importance to establish more accurate, rapid, and efficient diagnostic methods. Since infection with Mycobacterium tuberculosis (M. tb) is largely mediated through the respiratory tract, IgA responses against mycobacterial proteins are worthy of investigation for their potential clinical utility. In this study, the IgA response targeting lipoprotein Z (LppZ) was determined by using a homemade ELISA with plasma of TB patients (N = 125), LTBI individuals (N = 92), healthy controls (HCs) (N = 165), as well as TB patients undergoing anti-TB treatment (N = 9)...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29243878/predictive-value-of-c-reactive-protein-for-tuberculosis-bloodstream-infection-or-death-among-hiv-infected-individuals-with-chronic-non-specific-symptoms-and-negative-sputum-smear-microscopy
#10
Richard A Bedell, Monique van Lettow, Christopher Meaney, Elizabeth L Corbett, Adrienne K Chan, Robert S Heyderman, Suzanne T Anderson, Ann Åkesson, Moses Kumwenda, Rony Zachariah, Anthony D Harries, Andrew R Ramsay
BACKGROUND: To determine whether C-reactive protein (CRP) is an inflammatory biomarker that may identify patients at risk of infections or death. Mortality among HIV-infected persons commencing antiretroviral therapy (ART) is often attributed to tuberculosis (TB) or bloodstream infections (BSI). METHODS: In two district hospitals in southern Malawi we recruited HIV-infected adults with one or more unexplained symptoms present for at least one month (weight loss, fever, or diarrhea), and negative expectorated sputum microscopy for TB...
December 15, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/29230217/type-i-interferons-in-the-pathogenesis-of-tuberculosis-molecular-drivers-and-immunological-consequences
#11
REVIEW
Meg L Donovan, Thomas E Schultz, Taylor J Duke, Antje Blumenthal
Tuberculosis (TB) remains a major global health threat. Urgent needs in the fight against TB include improved and innovative treatment options for drug-sensitive and -resistant TB as well as reliable biological indicators that discriminate active from latent disease and enable monitoring of treatment success or failure. Prominent interferon (IFN) inducible gene signatures in TB patients and animal models of Mycobacterium tuberculosis infection have drawn significant attention to the roles of type I IFNs in the host response to mycobacterial infections...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29225091/gene-expression-profiling-of-the-trim-protein-family-reveals-potential-biomarkers-for-indicating-tuberculosis-status
#12
Yanqing Chen, Shuhui Cao, Yong Sun, Chuanyou Li
Tripartite motif (TRIM) family proteins play important regulatory roles in innate immune responses, the dysregulation of which cause several infectious diseases. However, the role and function of TRIM family proteins during tuberculosis (TB) infection remains unclear. In this study, we employed real-time quantitative PCR to profile the transcript levels of 72 TRIM genes from a cohort of 5 active TB patients, 5 latent tuberculosis infection (LTBI) subjects, and 5 healthy controls (HCs) in an initial discovery phase...
December 7, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29224833/hemostasis-and-lipoprotein-indices-signify-exacerbated-lung-injury-in-tuberculosis-with-diabetes-comorbidity
#13
Zhengwei Dong, Jingyun Shi, Anca Dorhoi, Jie Zhang, Adiilah K Soodeen-Lalloo, Wenlin Chen, Hongyun Yin, Wei Sha, Weitong Li, Ruijuan Zheng, Zhonghua Liu, Hua Yang, Lianhua Qin, Jie Wang, Xiaochen Huang, Chunyan Wu, Stefan H E Kaufmann, Yonghong Feng
BACKGROUND: Exacerbated immunopathology is a frequent consequence of tuberculosis (TB) that is complicated by diabetes mellitus (DM), yet the underlying mechanisms are still poorly defined. METHODS: In the two groups of age- and gender-matched TB patients with (DM-TB) and without DM (NDM-TB), we microscopically evaluated the areas of caseous necrosis and graded the extent of perinecrotic fibrosis in lung biopsies from the sputum smear-negative (SN) patients. We scored acid-fast bacilli in smear-positive (SP) patients and compiled computed tomography data from both SN-TB and SP-TB patients...
December 7, 2017: Chest
https://www.readbyqxmd.com/read/29208371/integrated-semi-targeted-metabolomics-analysis-reveals-distinct-metabolic-dysregulation-in-pleural-effusion-caused-by-tuberculosis-and-malignancy
#14
Nanying Che, Yan Ma, Huabin Ruan, Lina Xu, Xueying Wang, Xinting Yang, Xiaohui Liu
BACKGROUND: Tuberculous pleural effusion (TPE) and malignant pleural effusion (MPE) are the 2 most frequent causes of exudative pleural effusions (PEs). However, the clinical differentiation is challenging. METHODS: Metabolic signatures in pleural effusion from 156 patients were profiled. An integrated semi-targeted metabolomics platform was incorporated for high throughput metabolite identification and quantitation. In this platform, orbitrap based mass spectrometry with data dependent MS/MS acquisition was applied in the analysis...
December 5, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29196714/screening-and-identification-of-lncrnas-as-potential-biomarkers-for-pulmonary-tuberculosis
#15
Zhong-Liang Chen, Li-Liang Wei, Li-Ying Shi, Meng Li, Ting-Ting Jiang, Jing Chen, Chang-Ming Liu, Su Yang, Hui-Hui Tu, Yu-Ting Hu, Lin Gan, Lian-Gen Mao, Chong Wang, Ji-Cheng Li
Pulmonary tuberculosis (TB) is among the diseases with the highest morbidity and mortality worldwide. Effective diagnostic methods for TB are lacking. In this study, we investigated long non-coding RNAs (lncRNAs) in plasma using microarray and the potential diagnostic value of lncRNAs for TB. We found a total of 163 up-regulated lncRNAs and 348 down-regulated lncRNAs. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and coding-noncoding co-expression (CNC) analyses showed that functions of differentially expressed lncRNAs were mainly enriched in the regulation of alpha-beta T cell activation and the T cell receptor signalling pathway...
December 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29178041/cellular-immune-response-in-mdr-tb-patients-to-different-protein-expression-of-mdr-and-susceptible-mycobacterium-tuberculosis-rv0147-a-novel-mdr-tb-biomarker
#16
Alireza Hadizadeh Tasbiti, Shamsi Yari, Seyed Davar Siadat, Payam Tabarsi, Kayvan Saeedfar, Fatemeh Yari
Tuberculosis (TB) is a crucial public health problem with prevalence of multidrug resistant (MDR) rising. An accurate TB biomarker is urgently needed to monitor the response to treatment in patients with MDR tuberculosis. To analyze interaction between selected MDR-TB purified protein and immune cells, dendritic cells from MDR-TB patients and healthy subjects were stimulated by 55KDa protein fractions (Rv0147). The purified proteins identified by proteomic techniques (two-dimensional gel electrophoresis, mass spectrometry) and peptide sequences are known to bind a MHC class I alleles which are extracted from the Immune Epitope Database and Analysis Resource database ( www...
November 24, 2017: Immunologic Research
https://www.readbyqxmd.com/read/29174965/il-6-release-of-rv0183-antigen-stimulated-whole-blood-is-a-potential-biomarker-for-active-tuberculosis-patients
#17
Yongliang Liu, Xiaofei Li, Wei Liu, Yang Liu, Zhouyue Zhong, Lili Wang, ShengXiang Ge, Jun Zhang, Ningshao Xia
OBJECTIVE: New tests for diagnosing active tuberculosis (aTB) are urgently needed, and TB antigen-specific cell-mediated immunity can be expected to develop new testing methods of aTB. MATERIALS AND METHODS: Rv0183 protein, the only monoglyceride lipase identified in mycobacteria, was used to stimulate freshly heparin-treated whole blood. The Rv0183-specific cytokines/chemokines response associated with aTB was screened firstly with 4 aTB patients and 4 LTBIs, and further evaluated in 192 suspected aTB patients and 372 healthy individuals...
November 24, 2017: Journal of Infection
https://www.readbyqxmd.com/read/29170044/differential-gene-expression-in-mycobacterium-bovis-challenged-monocyte-derived-macrophages-of-cattle
#18
Sanjeev Kumar Shukla, Shubhra Shukla, Anuj Chauhan, Sarvjeet, Rehan Khan, Lakshya Veer Singh, Naveen Sharma, Chandan Prakash, Ajay Vir Singh, Manjit Panigrahi
A functional genomics approach was used to examine the immune response for transcriptional profiling of PBMC M. bovis infected cattle and healthy control cattle to stimulation with bovine tuberculin (purified protein derivative PPD-b). Total cellular RNA was extracted from non-challenged control and M. bovis challenged MDM for all animals at intervals of 6 h post-challenge, in response to in-vitro challenge with M. bovis (multiplicity of infection 2:1) and prepared for global gene expression analysis using the Agilent Bovine (V2) Gene Expression Microarray, 8 × 60 K...
November 20, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29160384/current-use-and-acceptability-of-novel-diagnostic-tests-for-active-tuberculosis-a-worldwide-survey
#19
Massimo Amicosante, Lia D'Ambrosio, Marcela Munoz, Fernanda Carvalho de Queiroz Mello, Marc Tebruegge, Novel Njweipi Chegou, Fouad Seghrouchni, Rosella Centis, Delia Goletti, Graham Bothamley, Giovanni Battista Migliori
OBJECTIVE: To determine the current use and potential acceptance (by tuberculosis experts worldwide) of novel rapid tests for the diagnosis of tuberculosis that are in line with World Health Organization target product profiles. METHODS: A multilingual survey was disseminated online between July and November of 2016. RESULTS: A total of 723 individuals from 114 countries responded to the survey. Smear microscopy was the most commonly used rapid tuberculosis test (available to 90...
September 2017: Jornal Brasileiro de Pneumologia: Publicaça̋o Oficial da Sociedade Brasileira de Pneumologia e Tisilogia
https://www.readbyqxmd.com/read/29129701/biomarkers-for-treatment-monitoring-in-tuberculosis-a-new-hope
#20
Subash Babu
No abstract text is available yet for this article.
December 2017: EBioMedicine
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