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https://www.readbyqxmd.com/read/27865622/protecting-from-envelope-stress-variations-on-the-phage-shock-protein-theme
#1
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Riccardo Manganelli, Maria Laura Gennaro
During envelope stress, critical inner-membrane functions are preserved by the phage-shock-protein (Psp) system, a stress response that emerged from work with Escherichia coli and other Gram-negative bacteria. Reciprocal regulatory interactions and multiple effector functions are well documented in these organisms. Searches for the Psp system across phyla reveals conservation of only one protein, PspA. However, examination of Firmicutes and Actinobacteria reveals that PspA orthologs associate with non-orthologous regulatory and effector proteins retaining functions similar to those in Gram-negative counterparts...
November 16, 2016: Trends in Microbiology
https://www.readbyqxmd.com/read/22337887/discovery-and-analysis-of-cofactor-dependent-phosphoglycerate-mutase-homologs-as-novel-phosphoserine-phosphatases-in-hydrogenobacter-thermophilus
#2
Yoko Chiba, Kenro Oshima, Hiroyuki Arai, Masaharu Ishii, Yasuo Igarashi
Phosphoserine phosphatase (PSP) catalyzes the dephosphorylation of phosphoserine to serine and inorganic phosphate. PSPs, which have been found in all three domains of life, belong to the haloacid dehalogenase-like hydrolase superfamily. However, certain organisms, particularly bacteria, lack a classical PSP gene, although they appear to possess a functional phosphoserine synthetic pathway. The apparent lack of a PSP ortholog in Hydrogenobacter thermophilus, an obligately chemolithoautotrophic and thermophilic bacterium, represented a missing link in serine anabolism because our previous study suggested that serine should be synthesized from phosphoserine...
April 6, 2012: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/20673359/sampling-and-pyrosequencing-methods-for-characterizing-bacterial-communities-in-the-human-gut-using-16s-sequence-tags
#3
Gary D Wu, James D Lewis, Christian Hoffmann, Ying-Yu Chen, Rob Knight, Kyle Bittinger, Jennifer Hwang, Jun Chen, Ronald Berkowsky, Lisa Nessel, Hongzhe Li, Frederic D Bushman
Intense interest centers on the role of the human gut microbiome in health and disease, but optimal methods for analysis are still under development. Here we present a study of methods for surveying bacterial communities in human feces using 454/Roche pyrosequencing of 16S rRNA gene tags. We analyzed fecal samples from 10 individuals and compared methods for storage, DNA purification and sequence acquisition. To assess reproducibility, we compared samples one cm apart on a single stool specimen for each individual...
July 30, 2010: BMC Microbiology
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