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https://www.readbyqxmd.com/read/28324160/regulation-of-blood-oxygen-transport-in-hibernating-mammals
#1
Inge G Revsbech, Angela Fago
Along with the periodic reductions in O2 requirements of mammalian hibernators during winter, the O2 affinity of the blood of mammalian hibernators is seasonally regulated to help match O2 supply to consumption, contributing to limit tissue oxidative stress, particularly at arousals. Specifically, mammalian hibernators consistently show an overall increase in the blood-O2 affinity, which causes a decreased O2 unloading to tissues, while having similar or lower tissue O2 tensions during hibernation. This overview explores how the decreased body temperature and concentration of red blood cell 2,3-diphosphoglycerate (DPG) that occur in hibernation contribute separately or in combination to the concurrent increase in the O2 affinity of the hemoglobin, the O2 carrier protein of the blood...
March 21, 2017: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/28260494/hemoglobin-and-myoglobin-as-reducing-agents-in-biological-systems-redox-reactions-of-globins-with-copper-and-iron-salts-and-complexes
#2
REVIEW
G B Postnikova, E A Shekhovtsova
In addition to reversible O2 binding, respiratory proteins of the globin family, hemoglobin (Hb) and myoglobin (Mb), participate in redox reactions with various metal complexes, including biologically significant ones, such as those of copper and iron. HbO2 and MbO2 are present in cells in large amounts and, as redox agents, can contribute to maintaining cell redox state and resisting oxidative stress. Divalent copper complexes with high redox potentials (E0, 200-600 mV) and high stability constants, such as [Cu(phen)2](2+), [Cu(dmphen)2](2+), and CuDTA oxidize ferrous heme proteins by the simple outer-sphere electron transfer mechanism through overlapping π-orbitals of the heme and the copper complex...
December 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28123493/experimental-studies-on-the-inactivation-of-hbv-in-blood-via-riboflavin-photochemical-treatment
#3
Zi-Yu Zhou, Xing-Xiu Bi
The aim of the study was to examine the influence of riboflavin photochemical method on the inactivation of hepatitis B virus (HBV) and the functions of red blood cells. Twenty patients suffering viral hepatitis B were selected in this study, and venous blood was collected and final concentration of 1,500 µmol/l riboflavin were added, to accept the λ=400-500 nm. The light intensity of 40,000 lux was treated with 2 h. The effect of inactivation was then evaluated and the function of red blood cells was detected...
January 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28073405/rhodanobacter-humi-sp-nov-a-novel-acidtolerant-and-alkalitolerant-gammaproteobacterium-isolated-from-kyonggi-university-forest-soil
#4
Ram Hari Dahal, Jaisoo Kim
A novel acidtolerant and alkalitolerant gammaproteobacterium designated strain RS22T was isolated from Kyonggi University forest soil. Cells were aerobic, Gram-staining-negative, catalase- and oxidase- positive, non-motile, non-spore-forming, rod-shaped, and yellow-pigmented. Flexirubin-type pigments were absent. Strain RS22T assimilates lactic acid, L-proline, and 3-hydroxybenzoic acid; tolerates 4 % of NaCl, ferments glucose, and can grow at pH 11.0. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RS22T formed a lineage within the class Gammaproteobacteria of the phylum Proteobacteria that was distinct from various species of the genus Rhodanobacter, including Rhodanobacter denitrificans 2APBS1T (97...
January 10, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28032538/alkalicoccus-halolimnae-gen-nov-sp-nov-a-moderately-halophilic-bacterium-isolated-from-a-salt-lake-and-reclassification-of-bacillus-saliphilus-as-alkalicoccus-saliphilus-comb-nov
#5
Baisuo Zhao, Weidong Lu, Shanshan Zhang, Kang Liu, Yanchun Yan, Jun Li
A Gram-stain-positive, cocci-shaped, non-spore-forming, and moderately halophilic bacterium, designed BZ-SZ-XJ29T, was isolated from a salt lake of China. On the basis of 16S rRNA gene sequence similarity, the most closely phylogenetic relatives were Bacillus saliphilus 6AGT (97.3 %) and other five Bacillus species (95.4-96.3 %). However, it shared only 89.5 % with Bacillus subtilis subsp. subtilis DSM 10T, indicating that this isolate might not be a member of Bacillus genus. The genomic DNA G+C content was 40...
December 29, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28001312/critical-re-appraisal-of-blood-component-quality-after-overnight-hold-of-whole-blood-outside-current-room-temperature-limits
#6
I J Bontekoe, P F van der Meer, D de Korte
BACKGROUND AND OBJECTIVES: According to European guidelines, the temperature of whole blood (WB) has to be maintained at 20-24°C until processing within 24 h, but in blood bank practice, WB is frequently held at temperatures between 18-25°C. We aimed to assess the impact of these small temperature deviations on the quality of the blood components. MATERIALS AND METHODS: After rapid cooling, 7 WB units were held overnight at 18°C and 8 units at 25°C, reflecting worst case holding conditions, and separated into a red cell concentrate (RCC), plasma and buffy coat (BC)...
February 2017: Vox Sanguinis
https://www.readbyqxmd.com/read/28001307/storage-of-red-blood-cells-in-a-novel-polyolefin-blood-container-a-pilot-in-vitro-study
#7
H Gulliksson, S Meinke, A Ravizza, L Larsson, P Höglund
BACKGROUND AND OBJECTIVES: The present general plasticizer di-2-ethylhexyl-phthalate in polyvinylchloride (PVC) blood bags is only physically dispersed in PVC and will therefore leach into blood components. The objective of this study was to perform a first preliminary red blood cell (RBC) storage evaluation in a new blood bag manufactured of polyolefin without any inclusion of potentially migrating substances. STUDY DESIGN AND METHODS: This is a RBC storage study for 42 days...
January 2017: Vox Sanguinis
https://www.readbyqxmd.com/read/28000961/adiabatic-excitation-for-31-p-mr-spectroscopy-in-the-human-heart-at-7-t-a-feasibility-study
#8
Ladislav Valkovič, William T Clarke, Lucian A B Purvis, Benoit Schaller, Matthew D Robson, Christopher T Rodgers
PURPOSE: Phosphorus magnetic resonance spectroscopy ((31) P-MRS) provides a unique tool for assessing cardiac energy metabolism, often quantified using the phosphocreatine (PCr)/adenosine triphosphate (ATP) ratio. Surface coils are typically used for excitation for (31) P-MRS, but they create an inhomogeneous excitation field across the myocardium, producing undesirable, spatially varying partial saturation. Therefore, we implemented adiabatic excitation in a 3D chemical shift imaging (CSI) sequence for cardiac (31) P-MRS at 7 Tesla (T)...
December 21, 2016: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/27993597/a-comparison-of-blood-nitric-oxide-metabolites-and-hemoglobin-functional-properties-among-diving-mammals
#9
Angela Fago, Daniel Garcia Parraga, Elin E Petersen, Niels Kristensen, Lea Giouri, Frank B Jensen
The ability of marine mammals to hunt prey at depth is known to rely on enhanced oxygen stores and on selective distribution of blood flow, but the molecular mechanisms regulating blood flow and oxygen transport remain unresolved. To investigate the molecular mechanisms that may be important in regulating blood flow, we measured concentration of nitrite and S-nitrosothiols (SNO), two metabolites of the vasodilator nitric oxide (NO), in the blood of 5 species of marine mammals differing in their dive duration: bottlenose dolphin, South American sea lion, harbor seal, walrus and beluga whale...
March 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/27959788/alcaligenes-endophyticus-sp-nov-isolated-from-roots-of-ammodendron-bifolium
#10
Chun-Yan Lu, Yu-Qian Li, Ye Tian, Ming-Xian Han, Manik Prabhu Narsing Rao, Yan-Ru Li, Zhi-Nan Zhu, Da-Qiao Wei, Deng-Di An, Wen-Jun Li
A Gram-staining negative, rod-shaped, motile bacterium, designated AER10T, was isolated from roots of Ammodendron bifolium collected from Takeermohuer desert in Xinjiang Uygur Autonomous Region, north western China. Growth was found to occur from 10°C to 45°C, at pH 5.0-9.0 and could tolerate NaCl up to 10 % (w/v). 16S rRNA gene sequence result indicated that the strain AER10T belongs to the genus Alcaligenes and was closely related to Alcaligenes aquatilis (98.4 %), Alcaligenes faecalis subsp. parafaecalis (98...
December 12, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27926823/aliidiomarina-soli-sp-nov-isolated-from-saline-alkaline-soil
#11
Lian Xu, Ji-Quan Sun, Li-Juan Wang, Xiao-Zhen Liu, Yin-Yao Ji, Zhong-Qiu Shao, Xiao-Lei Wu
A Gram-stain-negative, motile, non-spore-forming bacterium, designated strain Y4G10-17T, was isolated from the saline-alkali farmland top soil, Inner Mongolia, northern China. Strain Y4G10-17T could grow at 4-45 °C (with 30 °C as the optimal temperature), pH 6.0-12.0 (optimal at pH 9.0) and in the presence of 1.0-12.0 % (w/v) NaCl (optimal at 4.0-6.0 %). Phylogenetic analysis based on the eight different copies of the 16S rRNA gene sequences revealed that strain Y4G10-17T shared the highest sequence similarity with Aliidiomarina maris CF12-14T, 97...
April 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27717415/monitoring-oxygen-status
#12
REVIEW
J G Toffaletti, C R Rackley
Although part of a common "blood gas" test panel with pH and pCO2, the pO2, %O2Hb, and related parameters are independently used to detect and monitor oxygen deficits from a variety of causes. Measurement of blood gases and cooximetry may be done by laboratory analyzers, point of care testing, noninvasive pulse oximetry, and transcutaneous blood gases. The specimen type and mode of monitoring oxygenation that are chosen may be based on a combination of urgency, practicality, clinical need, and therapeutic objectives...
2016: Advances in Clinical Chemistry
https://www.readbyqxmd.com/read/27671055/paludifilum-halophilum-gen-nov-sp-nov-a-thermoactinomycete-isolated-from-superficial-sediment-of-a-solar-saltern
#13
Donyez Frikha-Dammak, Marie-Laure Fardeau, Jean-Luc Cayol, Lilia Ben Fguira-Fourati, Soumaya Najeh, Bernard Ollivier, Sami Maalej
A novel filamentous, halophilic, thermotolerant bacterium, strain SMBg3T was isolated from superficial sediment of a solar saltern in Sfax, Tunisia. The isolate is Gram-staining-positive, aerobic, catalase- and oxidase-positive. Optimum growth occurred at 40-45 °C, with 10 % (w/v) NaCl and at pH 8.0-9.0. Long and well developed aerial and substrate mycelia, with long chains of fluorescent and circular spores, were observed on all tested media. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SMBg3T belongs to an independent phylogenetic lineage of the family Thermoactinomycetaceae and shows a gene sequence similarity of 94 % with Desmospora activa DSM 45169T 94...
December 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27646145/altitudeomics-red-blood-cell-metabolic-adaptation-to-high-altitude-hypoxia
#14
Angelo D'Alessandro, Travis Nemkov, Kaiqi Sun, Hong Liu, Anren Song, Andrew A Monte, Andrew W Subudhi, Andrew T Lovering, Daniel Dvorkin, Colleen G Julian, Christopher G Kevil, Gopi K Kolluru, Sruti Shiva, Mark T Gladwin, Yang Xia, Kirk C Hansen, Robert C Roach
Red blood cells (RBCs) are key players in systemic oxygen transport. RBCs respond to in vitro hypoxia through the so-called oxygen-dependent metabolic regulation, which involves the competitive binding of deoxyhemoglobin and glycolytic enzymes to the N-terminal cytosolic domain of band 3. This mechanism promotes the accumulation of 2,3-DPG, stabilizing the deoxygenated state of hemoglobin, and cytosol acidification, triggering oxygen off-loading through the Bohr effect. Despite in vitro studies, in vivo adaptations to hypoxia have not yet been completely elucidated...
October 7, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27612938/pseudohongiellanitratireducens-sp-nov-isolated-from-seawater-and-emended-description-of-the-genus-pseudohongiella
#15
Lin Xu, Yue-Hong Wu, Shu-Ling Jian, Chun-Sheng Wang, Min Wu, Lei Cheng, Xue-Wei Xu
Two Gram-stain-negative, aerobic, motile by a single polar flagellum and rod-shaped strains, designated SCS-49T and SCS-111, were isolated from seawater of the South China Sea. The two strains grew at 4-35 °C, with 0.5-7.5 % (w/v) NaCl and at pH 6.5-9.0 and were able to reduce nitrate. Q-8 was the sole ubiquinone. The major fatty acids of the two strains were C16 : 0, C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospoglycolipid, three unidentified glycolipids, five unidentified phospholipids and two to three unidentified lipids...
December 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27521144/lysobacter-rhizophilus-sp-nov-isolated-from-rhizosphere-soil-of-mugunghwa-the-national-flower-of-south-korea
#16
Zheng-Fei Yan, Huan Trinh, Gabriela Moya, Pei Lin, Chang-Tian Li, Moo-Chang Kook, Tae-Hoo Yi
A Gram-staining-negative, aerobic, motile, rod-shaped, catalase- and oxidase-negative strain with one polar flagellum, designated THG-YS3.6T, was isolated from rhizosphere soil of a mugunghwa flower collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-37 °C (optimum 25-30 °C), at pH 6-8 (optimum 7.0) and with 0-2.0 % NaCl (optimum 1 %). The isoprenoid quinone was ubiquinone-8 (Q-8). The major cellular fatty acids were iso-C11  : 0, iso-C11 : 0 3-OH, iso-C15 : 0, iso-C16 : 0, C16 : 1ω7c alcohol, C16 : 0, iso-C17 : 0 and summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl)...
November 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27487516/the-effects-of-freezing-on-long-term-storage-of-canine-erythrocytes
#17
D Aktaran Bala, M Özcan
Human medicine studies have so far demonstrated that erythrocytes may be preserved and stored at low temperatures for decades retaining their metabolic and biochemical properties. However, detailed studies regarding this problem are not yet available in veterinary medicine. Therefore, the objective of the current study was to investigate time-dependent effects of long-term frozen storage of canine red blood cells. Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged by a cryogenic microcentrifuge and packed RBC suspensions were obtained...
2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/27226210/longibacter-salinarum-gen-nov-sp-nov-isolated-from-a-marine-solar-saltern
#18
Jun Xia, Christopher A Dunlap, Lina Flor-Weiler, Alejandro P Rooney, Guan-Jun Chen, Zong-Jun Du
A bacterial strain, designated WDS2C18T, was isolated from a marine solar saltern from the coast of Weihai, Shandong Province, China. Cells of strain WDS2C18T were long rod-shaped, red, and approximately 6.0-12.0 µm in length and 0.3-0.4 µm in width. The strain was Gram-stain-negative, facultatively anaerobic, heterotrophic, catalase-positive and oxidase-negative. Optimal growth was observed at 40 °C, at pH 7.5-8.0 with 8-12 % (w/v) NaCl. Nitrate was not reduced. Glycerol, sucrose, starch and d-mannitol stimulated growth, but not glucose, d-fructose, d-galactose, d-lactose, maltose, d-mannose, d-xylose, d-ribose, d-arabinose, l-rhamnose or cellobiose...
September 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27195751/the-diagnostic-challenge-of-group-2-pulmonary-hypertension
#19
REVIEW
Robert Naeije, Michele D'Alto
Pulmonary hypertension (PH) secondary to left heart diseases associated with an increased pulmonary venous pressure is the second of a total of five groups recognized in the classification of PH. Group 2 PH is the commonest form of PH, and is associated with high morbidity and mortality. The diagnosis of group 2 PH relies on a clinical probability assessment in which echocardiography plays a major role, eventually followed by the invasive measurements of a mean pulmonary artery pressure (mPAP) ≥25mmHg and a wedged PAP (PAWP) >15mmHg...
July 2016: Progress in Cardiovascular Diseases
https://www.readbyqxmd.com/read/27169721/oblitimonas-alkaliphila-gen-nov-sp-nov-in-the-family-pseudomonadaceae-recovered-from-a-historical-collection-of-previously-unidentified-clinical-strains
#20
Adam M Drobish, Brian D Emery, Anne M Whitney, Ana C Lauer, Maureen G Metcalfe, John R McQuiston
Eight Gram-stain-negative bacteria (B4199T, C6819, C6918, D2441, D3318, E1086, E1148 and E5571) were identified during a retrospective study of unidentified strains from a historical collection held in the Special Bacteriology Reference Laboratory at the Centers for Disease Control and Prevention. The strains were isolated from eight patients: five female, two male and one not specified. No ages were indicated for the patients. The sources were urine (3), leg tissue (2), foot wound, lung tissue and deep liver...
August 2016: International Journal of Systematic and Evolutionary Microbiology
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