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https://www.readbyqxmd.com/read/29156555/identification-of-major-flavone-c-glycosides-and-their-optimized-extraction-from-cymbidium-kanran-using-deep-eutectic-solvents
#1
Kyung Min Jeong, Misuk Yang, Yan Jin, Eun Mi Kim, Jaeyoung Ko, Jeongmi Lee
Cymbidium kanran, an orchid exclusively distributed in Northeast Asia, has been highly valued as a decorative plant and traditional herbal medicine. Here, C. kanran extracts were prepared in 70% aqueous methanol using ultrasound-assisted extraction (UAE) and subjected to liquid chromatography-photodiode array detection and ultra-high performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry analysis, which were used for quantitative and qualitative analysis, respectively. It was found that the extracts were rich in flavone C-glycosides including vicenin-2, vicenin-3, schaftoside, vitexin, and isovitexin...
November 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29148140/haemodynamic-effects-of-an-acute-vasodilator-challenge-in-heart-failure-patients-with-reduced-ejection-fraction-and-different-forms-of-post-capillary-pulmonary-hypertension
#2
Stefano Ghio, Gabriele Crimi, Pier Luigi Temporelli, Egidio Traversi, Maria Teresa La Rovere, Antonia Cannito, Dario Vizza, Laura Scelsi, Claudia Raineri, Marco Guazzi, Luigi Oltrona Visconti
AIMS: The most recent European guidelines have proposed new definitions of pulmonary hypertension (PH) in left heart disease, to better approach the characteristics required to reflect the presence of pulmonary vascular disease. The purpose of this study was to assess whether different haemodynamic definitions of post-capillary PH imply a different reversibility of PH in response to acute vasodilator administration in heart failure patients with reduced ejection fraction and PH (HFrEF-PH)...
November 16, 2017: European Journal of Heart Failure
https://www.readbyqxmd.com/read/29134941/bacillus-marinisedimentorum-sp-nov-isolated-from-marine-sediment
#3
Ling-Yun Guo, Si-Kai Ling, Chang-Ming Li, Guan-Jun Chen, Zong-Jun Du
A novel Gram-stain-positive, motile and facultatively anaerobic strain, designated NC2-31(T), was isolated from sediment from the coast of Weihai, PR China. Optimal growth occurred at 37 °C, pH 7.5 and with 2.0-3.0 % (w/v) NaCl. MK-7 was the major respiratory quinone. Meso-diaminopimelic acid was a diagnostic diamino acid in the peptidoglycan. The major polar lipids of NC2-31(T) were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). The genomic DNA G+C content of the strain was 46...
November 14, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29039306/paenibacillus-azotifigens-sp-nov-a-novel-nitrogen-fixing-bacterium-isolated-from-paddy-soil
#4
Muhammad Zubair Siddiqi, Gyu-Min Choi, Kang Duk Choi, Wan-Taek Im
A nitrogen-fixing bacterium, designated NF2-4-5(T), was isolated from a paddy soil in Anseong City, Korea. Cells of strain NF2-4-5(T) were Gram-staining-positive, motile rods and aerobic. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Paenibacillus and was closely related to Paenibacillus graminis RSA19(T) (98.7 %), Paenibacillus jilunlii Be17(T) (98.6 %), Paenibacillus salinicaeni LAM0A28(T) (98.6 %) and Paenibacillus riograndensis SBR5(T) (98...
October 17, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28981425/blood-quality-diagnostic-device-detects-storage-differences-between-donors
#5
Vivek P Jani, Shawn Mailo, Ali Athar, Alfredo Lucas, Alexander T Williams, Pedro Cabrales
In 2013, nearly 15 million units of banked blood were transfused in the United States of America alone. Blood shortages are expected to increase globally. Donated blood is not equal due to differences in quality and deterioration rate. There are no methods to detect time-dependent biochemical and biophysical changes of red blood cells (RBCs) or the deterioration rate of donated RBCs. Nine randomly selected RBC units collected by the San Diego Blood Bank were examined for interdonor variability over six weeks of storage...
October 5, 2017: IEEE Transactions on Biomedical Circuits and Systems
https://www.readbyqxmd.com/read/28935251/leachable-diphenylguanidine-from-rubber-closures-used-in-pre-filled-syringes-a-case-study-to-understand-solid-and-solution-interactions-with-oxytocin
#6
Ahmed S Zidan, Sabir M Aqueel, Alaadin Alayoubi, Adil Mohammad, Jinhui Zhang, Ziyaur Rahman, Patrick Faustino, Richard T Lostritto, Muhammad Ashraf
Leachables derived from multi-component drug-device syringe systems can result in changes to the quality of drug products. Diphenylguanidine (DPG), a leachable released from styrene butadiene rubber syringe plungers, interacts with Oxytocin to form protein-adducts. This study investigated the mechanism and kinetics of this interaction in both solid and solution states through in-vitro tests and spectroscopic methods For solid state interaction, the protein-adducts with DPG were characterized using SEM, XRD, DSC, FTIR, (13)C ss NMR, and dissolution analysis...
September 19, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28910585/analysis-of-glycols-glycol-ethers-and-other-volatile-organic-compounds-present-in-household-water-based-hand-pump-sprays
#7
Tsuyoshi Kawakami, Kazuo Isama, Toshiko Tanaka-Kagawa, Hideto Jinnno
The aim of this investigation is to clarify the types and concentrations of VOCs present in various commercial household water-based hand pump spray products used in Japan, and to estimate their average concentrations in indoor air when the spray product is used. We selected glycol and glycol ethers as the main target compounds, as these chemicals were detected at high frequencies and concentrations in a national survey of Japanese indoor air pollution. The extraction of these chemicals using graphite carbon cartridges was examined, with good recoveries and reproducibilities being obtained...
November 10, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28879839/ramlibacter-rhizophilus-sp-nov-isolated-from-rhizosphere-soil-of-national-flower-mugunghwa-from-south-korea
#8
Zheng-Fei Yan, Huan Trinh, Gabriela Moya, Pei Lin, Chang-Tian Li, MooChang Kook, Tae-Hoo Yi
A Gram-negative, aerobic, non-motile, long rods or coccoid without flagellum strain, designated THG-YS3.2.7(T), was isolated from therhizosphere soil of a Mugunghwa flower collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-40 °C (optimum 28-37 °C), at pH 6-8 (optimum 7) and at 0-5 % NaCl (optimum 1 %). The predominant ubiquinone was ubiquinone 8 (Q-8). The major cellular fatty acids were C10 : 0, C10 : 0 3OH, C16 : 0, C17 : 0, C17 : 0 cyclo, C18 : 0, C18 : 3ω6c (6,9,12), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1ω6c)...
October 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28851819/o2-binding-and-co2-sensitivity-in-hemoglobins-of-subterranean-african-mole-rats
#9
Roy E Weber, Jennifer U M Jarvis, Angela Fago, Nigel C Bennett
Inhabiting deep and sealed subterranean burrows, mole rats exhibit a remarkable suite of specializations, including eusociality (living in colonies with single breeding queens), extraordinary longevity, cancer immunity and poikilothermy, and extreme tolerance of hypoxia and hypercapnia.With little information available on adjustments in hemoglobin (Hb) function that may mitigate the impact of exogenous and endogenous constraints on the uptake and internal transport of O2, we measured hematological characteristics, as well as Hb-O2 binding affinities and their sensitivities to pH (Bohr effect), CO2, temperature and 2,3 diphosphoglycerate (DPG, the major allosteric modulator of Hb-O2 affinity in the red cells) in four social and two solitary species of African mole rats (family Bathyergidae) originating from different biomes and soil types across Central and Southern Africa...
August 29, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28801583/effect-of-x-ray-irradiation-on-hepatocarcinoma-cells-and-erythrocytes-in-salvaged-blood
#10
Feng-Jiang Zhang, Jin-Ting Yang, Li-Hui Tang, Wen-Na Wang, Kai Sun, Yue Ming, Kanhar Ghulam Muhammad, Yin-Fei Zheng, Min Yan
The broad clinical acceptance of intraoperative blood salvage and its applications in cancer surgery remain controversial. Until now, a method that can safely eliminate cancer cells while preserving erythrocytes does not exist. Here, we investigated whether X-ray generated from linear accelerator irradiation at a certain dose can kill hepatocarcinoma cells while preserving erythrocytes. HepG2, SK-Hep1 or Huh7 cells were mixed into the aliquots of erythrocytes obtained from healthy volunteers. After the mixed cells were exposed to 30 Gy and 50 Gy X-rays irradiation, the viability, clonogenicity, DNA synthesis and tumorigenicity of the tumor cells were determined by the MTT assay, plate colony formation, 5-ethynyl-2'-deoxyuridine incorporation, and subcutaneous xenograft implantation into immunocompromised mice...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28711785/a-fit-for-purpose-lc-ms-ms-method-for-the-simultaneous-quantitation-of-atp-and-2-3-dpg-in-human-k2edta-whole-blood
#11
Hyeryun Kim, Penelope Kosinski, Charles Kung, Lenny Dang, Yue Chen, Hua Yang, Yuan-Shek Chen, Jordyn Kramer, Guowen Liu
Many hemolytic anemias results in major metabolic abnormalities: two common metabolite abnormalities include increased levels of 2,3-diphosphoglycerate (2,3-DPG) and decreased levels of adenosine triphosphate (ATP). To better monitor the concentration changes of these metabolites, the development of a reliable LC-MS/MS method to quantitatively profile the concentrations of 2, 3-DPG and ATP in whole blood is essential to understand the effects of investigational therapeutics. Accurate quantification of both compounds imposes great challenges to bioanalytical scientists due to their polar, ionic and endogenous nature...
September 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28653084/bacillus-alcaliphilum-sp-nov-a-bacterium-isolated-from-a-soda-lake
#12
Vishnuvardhan Reddy Sultanpuram, Thirumala Mothe, Sasikala Chintalapati, Venkata Ramana Chintalapati
Two novel (14B(T) and 7B) Gram-stain-positive, rod-shaped, motile and endospore-forming bacterial strains were isolated from Lonar soda lake, India. Based on 16S rRNA gene sequence analysis, the strains 14B(T) and 7B were identified as belonging to the class Firmibacteria and were most closely related to Bacillus halodurans LMG 7121(T) (99.7 and 99.8%, respectively), Bacillus okuhidensis LMG 22468(T) (99.1 and 99.2%, respectively) and other members in the genus Bacillus (<97.0%). However, the DNA-DNA relatedness studies indicated that the strains 14B(T) and 7B were distantly related to B...
June 26, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28598831/rat-red-blood-cell-storage-lesions-in-various-additive-solutions
#13
Vivek P Jani, Ozlem Yalcin, Alexander T Williams, Mark A Popovsky, Pedro Cabrales
BACKGROUND: Small rodent models are routinely used to evaluate the safety and efficacy of blood transfusions. Limited comprehensive literature exists about effect of different storage solutions in rat red blood cells (RBCs) characteristics. RBCs undergo time dependent biochemical and biophysical changes during storage known as hypothermic storage lesions (HSLs). OBJECTIVE: This study evaluates the effects of RBC additive solutions (AS) during storage of rat RBCs...
2017: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/28471090/the-prognostic-importance-of-the-diastolic-pulmonary-gradient-transpulmonary-gradient-and-pulmonary-vascular-resistance-in-patients-undergoing-transcatheter-aortic-valve-replacement
#14
Nathan W Brunner, Su Fei Yue, Dion Stub, Jian Ye, Anson Cheung, Jonathon Leipsic, Sandra Lauck, Danny Dvir, Gidon Perlman, Nay Htun, Peter Fahmy, Roshan Prakash, Lim Eng, Kevin Ong, Michael Tsang, John A Cairns, John G Webb, David A Wood
OBJECTIVES: To evaluate the association between markers of precapillary pulmonary hypertension (PH) and survival in transcatheter aortic valve replacement (TAVR). BACKGROUND: The importance of precapillary PH has been sparsely investigated in patients undergoing TAVR. It may prove an important risk factor for poor outcomes. METHODS: We identified patients at our institution undergoing TAVR with a baseline right heart catheterization (RHC) demonstrating PH...
May 4, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/28324160/regulation-of-blood-oxygen-transport-in-hibernating-mammals
#15
Inge G Revsbech, Angela Fago
Along with the periodic reductions in O2 requirements of mammalian hibernators during winter, the O2 affinity of the blood of mammalian hibernators is seasonally regulated to help match O2 supply to consumption, contributing to limit tissue oxidative stress, particularly at arousals. Specifically, mammalian hibernators consistently show an overall increase in the blood-O2 affinity, which causes a decreased O2 unloading to tissues, while having similar or lower tissue O2 tensions during hibernation. This overview explores how the decreased body temperature and concentration of red blood cell 2,3-diphosphoglycerate (DPG) that occur in hibernation contribute separately or in combination to the concurrent increase in the O2 affinity of the hemoglobin, the O2 carrier protein of the blood...
July 2017: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/28260494/hemoglobin-and-myoglobin-as-reducing-agents-in-biological-systems-redox-reactions-of-globins-with-copper-and-iron-salts-and-complexes
#16
REVIEW
G B Postnikova, E A Shekhovtsova
In addition to reversible O2 binding, respiratory proteins of the globin family, hemoglobin (Hb) and myoglobin (Mb), participate in redox reactions with various metal complexes, including biologically significant ones, such as those of copper and iron. HbO2 and MbO2 are present in cells in large amounts and, as redox agents, can contribute to maintaining cell redox state and resisting oxidative stress. Divalent copper complexes with high redox potentials (E0, 200-600 mV) and high stability constants, such as [Cu(phen)2](2+), [Cu(dmphen)2](2+), and CuDTA oxidize ferrous heme proteins by the simple outer-sphere electron transfer mechanism through overlapping π-orbitals of the heme and the copper complex...
December 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28123493/experimental-studies-on-the-inactivation-of-hbv-in-blood-via-riboflavin-photochemical-treatment
#17
Zi-Yu Zhou, Xing-Xiu Bi
The aim of the study was to examine the influence of riboflavin photochemical method on the inactivation of hepatitis B virus (HBV) and the functions of red blood cells. Twenty patients suffering viral hepatitis B were selected in this study, and venous blood was collected and final concentration of 1,500 µmol/l riboflavin were added, to accept the λ=400-500 nm. The light intensity of 40,000 lux was treated with 2 h. The effect of inactivation was then evaluated and the function of red blood cells was detected...
January 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28073405/rhodanobacter-humi-sp-nov-an-acid-tolerant-and-alkalitolerant-gammaproteobacterium-isolated-from-forest-soil
#18
Ram Hari Dahal, Jaisoo Kim
A novel acid-tolerant and alkalitolerant gammaproteobacterium designated strain RS22T was isolated from Kyonggi University forest soil. Cells were aerobic, Gram-stain-negative, catalase- and oxidase-positive, non-motile, non-spore-forming, rod-shaped and yellow-pigmented. Flexirubin-type pigments were absent. Strain RS22T was able to assimilate lactic acid, l-proline and 3-hydroxybenzoic acid; it tolerated 4 % (w/v) NaCl, fermented glucose and was able to grow at pH 11.0. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RS22T formed a lineage within the class Gammaproteobacteria of the phylum Proteobacteria that was distinct from various species of the genus Rhodanobacter, including Rhodanobacter denitrificans 2APBS1T (97...
May 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28032538/reclassification-of-bacillus-saliphilus-as-alkalicoccus-saliphilus-gen-nov-comb-nov-and-description-of-alkalicoccus-halolimnae-sp-nov-a-moderately-halophilic-bacterium-isolated-from-a-salt-lake
#19
Baisuo Zhao, Weidong Lu, Shanshan Zhang, Kang Liu, Yanchun Yan, Jun Li
A Gram-stain-positive, cocci-shaped, non-spore-forming and moderately halophilic bacterium, designed BZ-SZ-XJ29T, was isolated from a salt lake of China. On the basis of 16S rRNA gene sequence similarity, the closest phylogenetic relatives were Bacillus saliphilus 6AGT (97.3 % 16S rRNA gene sequence similarity) and five other species of the genus Bacillus(95.4-96.3 %). However, strain BZ-SZ-XJ29T shared only 89.5 % 16S rRNA gene sequence similarity with Bacillus subtilis subsp. subtilis DSM 10T, indicating that this isolate might not be a member of the genus Bacillus...
May 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28001312/critical-re-appraisal-of-blood-component-quality-after-overnight-hold-of-whole-blood-outside-current-room-temperature-limits
#20
I J Bontekoe, P F van der Meer, D de Korte
BACKGROUND AND OBJECTIVES: According to European guidelines, the temperature of whole blood (WB) has to be maintained at 20-24°C until processing within 24 h, but in blood bank practice, WB is frequently held at temperatures between 18-25°C. We aimed to assess the impact of these small temperature deviations on the quality of the blood components. MATERIALS AND METHODS: After rapid cooling, 7 WB units were held overnight at 18°C and 8 units at 25°C, reflecting worst case holding conditions, and separated into a red cell concentrate (RCC), plasma and buffy coat (BC)...
February 2017: Vox Sanguinis
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