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2,3 DPG

J G Toffaletti, C R Rackley
Although part of a common "blood gas" test panel with pH and pCO2, the pO2, %O2Hb, and related parameters are independently used to detect and monitor oxygen deficits from a variety of causes. Measurement of blood gases and cooximetry may be done by laboratory analyzers, point of care testing, noninvasive pulse oximetry, and transcutaneous blood gases. The specimen type and mode of monitoring oxygenation that are chosen may be based on a combination of urgency, practicality, clinical need, and therapeutic objectives...
2016: Advances in Clinical Chemistry
Donyez Frikha-Dammak, Marie-Laure Fardeau, Jean-Luc Cayol, Lilia Ben Fguira-Fourati, Soumaya Najeh, Bernard Ollivier, Sami Maalej
A novel filamentous, halophilic, thermotolerant bacterium, strain SMBg3T was isolated from superficial sediment of a solar saltern in Sfax, Tunisia. The isolate is Gram-staining-positive, aerobic, catalase andoxidase-positive. Its optimum growth occurred at 40-45 °C, 10 % (w/v) NaCl and pH 8.0-9.0. Long and well developed aerial and substrate mycelia, with long chains of fluorescent and circular spores, were observed on all tested media. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SMBg3T belongs to an independent phylogenetic lineage of the family Thermoactinomycetaceae and shows a gene sequence similarity of 94 % with Desmospora activa, 94...
September 25, 2016: International Journal of Systematic and Evolutionary Microbiology
Angelo D'Alessandro, Travis Nemkov, Kaiqi Sun, Hong Liu, Anren Song, Andrew A Monte, Andrew W Subudhi, Andrew T Lovering, Daniel Dvorkin, Colleen G Julian, Christopher G Kevil, Gopi K Kolluru, Sruti Shiva, Mark T Gladwin, Yang Xia, Kirk C Hansen, Robert C Roach
Red blood cells (RBCs) are key players in systemic oxygen transport. RBCs respond to in vitro hypoxia through the so-called oxygen-dependent metabolic regulation, which involves the competitive binding of deoxyhemoglobin and glycolytic enzymes to the N-terminal cytosolic domain of band 3. This mechanism promotes the accumulation of 2,3-DPG, stabilizing the deoxygenated state of hemoglobin, and cytosol acidification, triggering oxygen off-loading through the Bohr effect. Despite in vitro studies, in vivo adaptations to hypoxia have not yet been completely elucidated...
October 7, 2016: Journal of Proteome Research
Lin Xu, Yue-Hong Wu, Shu-Ling Jian, Chun-Sheng Wang, Min Wu, Lei Cheng, Xue-Wei Xu
Two Gram-stain-negative, aerobic, motile by a single polar flagellum and rod-shaped strains, designated SCS-49T and SCS-111, were isolated from seawater of the South China Sea. The two strains grew at 4-35 °C with 0.5-7.5 % (w/v) NaCl and pH 6.5-9.0 and were able to reduce nitrate. Q-8 was the sole ubiquinone. The major fatty acids of both strains were C16:0, C18:1ω7c and summed feature 3 (C16:1ω7c and/or C16:1ω6c). The polar lipids include DPG, PE, PG, PGL, three unidentified glycolipids, five unidentified phospholipids and two to three unidentified lipids...
September 9, 2016: International Journal of Systematic and Evolutionary Microbiology
Zheng-Fei Yan, Huan Trinh, Gabriela Moya, Pei Lin, Chang-Tian Li, Moo-Chang Kook, Tae-Hoo Yi
A Gram-negative, aerobic, motile, rod with one polar flagellum, catalase and oxidase-negative strain, designated THG-YS3.6T, was isolated from rhizosphere soil of flower Mugunghwa collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-37 oC (optimum 25-30 oC), at pH 6-8 (optimum 7.0) and at 0-2.0% NaCl (optimum 1%). The isoprenoid quinone was ubiquinone-8 (Q-8). The major cellular fatty acids were iso-C11:0, iso-C11:0 3OH, iso-C15:0, iso-C16:0, C16:1 7c alcohol, C16:0, iso-C17:0, summed feature 9 (iso-C17:1 9c and/or C16:0 10-methyl)...
August 12, 2016: International Journal of Systematic and Evolutionary Microbiology
D Aktaran Bala, M Özcan
Human medicine studies have so far demonstrated that erythrocytes may be preserved and stored at low temperatures for decades retaining their metabolic and biochemical properties. However, detailed studies regarding this problem are not yet available in veterinary medicine. Therefore, the objective of the current study was to investigate time-dependent effects of long-term frozen storage of canine red blood cells. Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged by a cryogenic microcentrifuge and packed RBC suspensions were obtained...
2016: Polish Journal of Veterinary Sciences
Jun Xia, Christopher A Dunlap, Lina Flor-Weiler, Alejandro P Rooney, Guan-Jun Chen, Zong-Jun Du
A bacterial strain, designated WDS2C18T, was isolated from a marine solar saltern in the coast of Weihai, Shandong Province, PR China. Cells of strain WDS2C18T were long rod-shaped, red, and approximately 6.0-12.0 µm in length and 0.3-0.4 µm in width. The strain was Gram-stain-negative, facultatively anaerobic, heterotrophic, catalase-positive and oxidase-negative. Optimal growth was observed at 40 °C, pH 7.5-8.0, with 8-12% (w/v) NaCl. Nitrate was not reduced. Glycerol, sucrose, starch and D-mannitol stimulated growth, but not glucose, D-fructose, D-galactose, D-lactose, D-maltose, D-mannose, D-xylose, D-ribose, D-arabinose, L-rhamnose or D-cellobiose...
May 25, 2016: International Journal of Systematic and Evolutionary Microbiology
Robert Naeije, Michele D'Alto
Pulmonary hypertension (PH) secondary to left heart diseases associated with an increased pulmonary venous pressure is the second of a total of five groups recognized in the classification of PH. Group 2 PH is the commonest form of PH, and is associated with high morbidity and mortality. The diagnosis of group 2 PH relies on a clinical probability assessment in which echocardiography plays a major role, eventually followed by the invasive measurements of a mean pulmonary artery pressure (mPAP) ≥25mmHg and a wedged PAP (PAWP) >15mmHg...
July 2016: Progress in Cardiovascular Diseases
Adam M Drobish, Brian D Emery, Anne M Whitney, Ana C Lauer, Maureen G Metcalfe, John R McQuiston
Eight Gram-stain-negative bacteria (B4199T, C6819, C6918, D2441, D3318, E1086, E1148 and E5571) were identified during a retrospective study of unidentified strains from a historical collection held in the Special Bacteriology Reference Laboratory at the Centers for Disease Control and Prevention. The strains were isolated from eight patients: five female, two male and one not specified. No ages were indicated for the patients. The sources were urine (3), leg tissue (2), foot wound, lung tissue and deep liver...
August 2016: International Journal of Systematic and Evolutionary Microbiology
Vishnuvardhan Reddy Sultanpuram, Thirumala Mothe
Two novel (S9T and S12) Gram-stain-positive, rod shaped, non-motile and endospore forming bacteria were isolated from Narayan sarovar Lake, in India. The high 16S rRNA gene sequence similarity (99.9 %) and DNA-DNA relatedness (86 + 2 %) indicated that strains S9T and S12 were members of a single species. Based on the 16S rRNA gene sequence analysis these strains were identified as belonging to the class Firmibacteria and were most closely related to Bacillus agaradhaerens PN-105T (96.8 % sequence similarity), Bacillus neizhouensis JSM 071004T (96...
April 26, 2016: International Journal of Systematic and Evolutionary Microbiology
Y Subhash, John J Bang, Taek H You, Sang-Seob Lee
Strain S3T was isolated from the lagoon sediments, and appeared as transparent colonies on agar plates with cells staining Gram-negative. Catalase and oxidase were positive. Strain S3T hydrolyzed starch, casein and tween-20 while urea, chitin, gelatin and tween-80 was not. C18:1ω6c/C18:1ω7c, C16:16c/C16:17c, cyclo-C17:0 and C16:0 were predominant fatty acids with minor amounts of C10:0 -3OH, C12:0, C14:0 and C16:0 ¬-2OH. Strain S3T contained diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE) as major polar lipids with minor amounts of unidentified phospholipid (PL) and unidentified lipids (L1-2)...
April 26, 2016: International Journal of Systematic and Evolutionary Microbiology
Shanshan Zhang, Zhaojun Li, Yanchun Yan, Chuanlun Zhang, Jun Li, Baisuo Zhao
A Gram-stain-positive, rod-shaped, aerobic and moderately haloalkaliphilic bacterium, designated BZ-SZ-XJ18T, was isolated from the mixed water and sediment of a saline-alkaline lake located in the Xinjiang Uyghur Autonomous Region of China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BZ-SZ-XJ18T was a member of the genus Bacillus. The closest phylogenetic relatives were Bacillus saliphilus 6AGT (96.7 % 16S rRNA gene sequence similarity), 'Bacillusdaqingensis' X10-1 (96.6 %), Bacillus luteus JC167T (96...
June 2016: International Journal of Systematic and Evolutionary Microbiology
Xiaoming Zhou, Xuliang Zhang, Ying Zhang, Shuqing Hong, Lin Li, Zhong Liu
BACKGROUND: Previous studies have demonstrated that human RBCs can survival from freeze-drying, but there is still some uncertainty on whether and how the cell properties change during the processes. OBJECTIVE: The present study is to evaluate the effects of lyophilization and rehydration on the membrane surface antigens, such as CD antigens and blood group antigens, of human RBCs. MATERIALS AND METHODS: Human RBCs were lyophilized first through a simplified protocol, in which glycerol were used in the lyoprotectant solution instead of trehalose...
January 2016: Cryo Letters
Brian Hung-Hin Lang, Diane T Y Chan, Felix Che-Lok Chow, Kai Pun Wong, Rita Y K Chang
BACKGROUND: It remains uncertain whether a parathyroid gland (PG) that appears darkened or severely bruised but still has an attached vascular pedicle should be left in situ or taken out and auto-transplanted following total thyroidectomy. Our study aimed to examine the impact of discolored PGs (DPGs) on short- and long-term hypoparathyroidism. METHODS: One hundred and three patients who underwent total thyroidectomy with 4 clearly identified PGs were analyzed. Location (superior/inferior) and color of each PG were recorded...
July 2016: World Journal of Surgery
Andrew V Scott, Enika Nagababu, Daniel J Johnson, Khaled M Kebaish, Joshua A Lipsitz, Ian M Dwyer, Gabriel S Zuckerberg, Viachaslau M Barodka, Dan E Berkowitz, Steven M Frank
BACKGROUND: Stored red blood cells (RBCs) are deficient in 2,3-diphosphoglycerate (2,3-DPG), but it is unclear how autologous salvaged blood (ASB) compares with stored blood and how rapidly 2,3-DPG levels return to normal after transfusion. Therefore, we compared levels of 2,3-DPG in stored versus ASB RBCs and in patients' blood after transfusion. METHODS: Twenty-four patients undergoing multilevel spine fusion surgery were enrolled. We measured 2,3-DPG and the oxyhemoglobin dissociation curve (P50) in samples taken from the ASB and stored blood bags before transfusion and in blood samples drawn from patients before and after transfusion...
March 2016: Anesthesia and Analgesia
Nusratgul Anwar, Gulsimay Abaydulla, Bolatkhan Zayadan, Mehfuzem Abdurahman, Buayshem Hamood, Raziya Erkin, Nurimangul Ismayil, Manziram Rozahon, Hormathan Mamtimin, Erkin Rahman
A Gram-stain negative, aerobic, non-motile, short rod-shaped bacterium, designated KBL-4-9T, was isolated from the storage liquid in the stems of Populus euphratica trees in Xinjiang, PR China. Strain KBL-4-9T grew at 4-45 ℃ (optimum 37 ℃), 1-3 % (w/v) NaCl (optimum 1 %, w/v) and pH 5.5-9.5 (optimum pH 7.5). Phylogentic analyses based on 16S rRNA gene sequences indicated that strain KBL-4-9T belonged to the genus Pseudomonas and showed the highest 16S rRNA gene sequence similarity of 97.36 % to Pseudomonas pelagia CL-AP6T, followed by Pseudomonas xinjiangensis S3-3T (97...
January 18, 2016: International Journal of Systematic and Evolutionary Microbiology
Xia Qiu, Zhihao Qu, Fan Jiang, Yingjun Lin, Yumin Zhang, Xulu Chang, Xuyang Da, Sangsang Deng, Myongchol Kim, Chengxiang Fang, Fang Peng
A novel psychrotolerant bacterium, designed strain M6-79T, was isolated from an arctic glacial foreland soil sample collected from Ny-Ålesund in the Svalbard Archipelago, Norway. Cells of strain M6-79T were Gram-stain-negative, rod-shaped and produced a red-pigment. Strain M6-79T was strictly aerobic, non-motile, non-endospore-forming, oxidase-negative and catalase-positive. Based on the 16S rRNA gene sequence analysis, strain M6-79T was phylogenetically related to Roseomonas aquatica TR53T (95.2 %), Roseomonas lacus TH-G33T (94...
December 23, 2015: International Journal of Systematic and Evolutionary Microbiology
Rui Zhong, Hua Liu, Hong Wang, Xiaojuan Li, Zeng He, Meiduo Gangla, Jingdan Zhang, Dingding Han, Jiaxin Liu
Hypoxia has been reported to cause the significant enhancement of hemoglobin (Hb) and hematocrit (Hct), which stabilizes at relatively high levels after an individual ascends to a high altitude. However, the quality of the suspended red blood cells (SRBCs) obtained from individuals at high altitudes such as Tibetan plateau migrants after storage has not been studied. In this study, we compared the storage quality of SRBCs prepared from Tibetan plateau and Deyang lowland populations by adding a normal volume of mannitol-adenine-phosphate (MAP), which is a common additive solution used in blood storage in Asian countries...
2015: PloS One
Lei Song, Hongcan Liu, Jian Wang, Ying Huang, Xin Dai, Xiqiu Han, Yuguang Zhou
Two Gram-staining-positive, endospore-forming, rod-shaped bacterial strains, designated as strain SW109T and strain W006, were isolated from a seawater sample collected from the Indian Ocean. The strains were strictly aerobic, catalase- and oxidase-positive and motile by peritrichous flagella. The predominant cellular fatty acids (> 10%) were anteiso-C15: 0, iso-C15: 0 and iso-C14: 0. The major menaquinone was menaquinone-7 (MK-7) and the major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE)...
November 26, 2015: International Journal of Systematic and Evolutionary Microbiology
Kunho Kim, Sathiyaraj Srinivasan, Sang-Seob Lee
Two novel bacterial strains, designated C5(T) and C9 were isolated from a fish cage at Tongyeong, South Korea and were characterized to determine their taxonomic position. The strains were Gram-negative, non-motile, strictly aerobic and short rod shaped. Growth occurred between 20 and 32 °C (optimum 30 °C) and at pH 6.0-9.0 (optimum pH 8.0) and at 0-10 % NaCl (optimum at 2 % NaCl). Based on the 16S rRNA gene sequence analysis, they were identified as a member of the genus Loktanella that belongs to the phylum Proteobacteria...
February 2016: Current Microbiology
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