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Cardiomyocyte Cell Culture

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https://www.readbyqxmd.com/read/28928797/role-of-mir-128-in-hypertension-induced-myocardial-injury
#1
Jie Yin, Hongyan Liu, Lei Huan, Suping Song, Liying Han, Faxin Ren, Zengtang Zhang, Zhiqiang Zang, Junye Zhang, Shu Wang
The present study aimed to investigate the role and mechanism of micro RNA (miR)-128 in hypertension-induced myocardial injury. The peripheral blood of patients with hypertension was collected and the expression of miR-128 was detected using fluorescence reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Primary myocardial cells isolated from rat in vitro were cultured under conditions of hypoxia and glucose deprivation, and miR-128 expression was measured by RT-qPCR. The expression of c-Met protein was measured using western blot analysis and the apoptosis of transfected cells was measured by flow cytometry in rat myocardial cells following transfection with miR-128 mimics or c-Met siRNA...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28928480/bisoprolol-protects-myocardium-cells-against-ischemia-reperfusion-injury-by-attenuating-unfolded-protein-response-in-rats
#2
Chengcheng Zhang, Songqing He, Yanming Li, Feng Li, Zhengbing Liu, Jing Liu, Jianbin Gong
Bisoprolol (B) exerts potential cardioprotective effects against myocardial ischemia/reperfusion (I/R) injury. Unfolded protein response (UPR) attenuates I/R injury induced apoptosis by reducing oxidative damage and inflammation response. The current study investigated whether the protective effects of bisoprolol resulted from modulating UPR and anti-inflammatory during myocardial I/R condition and elucidated its potential mechanisms. Sprague-Dawley rats were treated with B in the absence or presence of the injected UPR activator dithiothreitol (DTT) and then subjected to myocardial I/R surgery...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28920097/acute-cd47-blockade-during-ischemic-myocardial-reperfusion-enhances-phagocytosis-associated-cardiac-repair
#3
Shuang Zhang, Xin-Yi Yeap, Matthew DeBerge, Nivedita K Naresh, Kevin Wang, Zhengxin Jiang, Jane E Wilcox, Steven M White, John P Morrow, Paul W Burridge, Daniel Procissi, Evan A Scott, William Frazier, Edward B Thorp
Our data suggest that, after a myocardial infarction, integrin-associated protein CD47 on cardiac myocytes is elevated. In culture, increased CD47 on the surface of dying cardiomyocytes impairs phagocytic removal by immune cell macrophages. After myocardial ischemia and reperfusion, acute CD47 inhibition with blocking antibodies enhanced dead myocyte clearance by cardiac phagocytes and also improved the resolution of cardiac inflammation, reduced infarct size, and preserved cardiac contractile function. Early targeting of CD47 in the myocardium after reperfusion may be a new strategy to enhance wound repair in the ischemic heart...
August 2017: JACC. Basic to Translational Science
https://www.readbyqxmd.com/read/28912851/molecular-mechanism-of-mir-181b-in-heart-disease-due-to-pregnancy-induced-hypertension-syndrome
#4
Zheng Gao, Li Wang, Jinyun Wang, Fengyong Yang, Jin Qu
The present study aimed to investigate the molecular mechanisms of microRNA (miR)-181b in heart disease due to hypertensive disorders complicating pregnancy (HDCP) through regulating the expression of metallopeptidase inhibitor 3 (TIMP3). miR-181b expression was detected by reverse transcription-quantitative polymerase chain reaction in peripheral blood samples from patients with HDCP. These samples were analyzed for clinical pathological characteristics. The primary cardiomyocytes of rats were cultured in hypoxic conditions for 24 h, in which miR-181b expression was detected at different time points...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28889965/losartan-counteracts-the-effects-of-cardiomyocyte-swelling-on-glucose-uptake-and-insulin-receptor-substrate-1-levels
#5
Yamil Gerena, Janice Griselle Lozada, Bryan Jael Collazo, Jarold Méndez-Álvarez, Jennifer Méndez-Estrada, Walmor C De Mello
A growing body of evidence demonstrates an association between Angiotensin II (Ang II) receptor blockers (ARBs) and enhanced glucose metabolism during ischemic heart disease. Despite these encouraging results, the mechanisms responsible for these effects during ischemia remain poorly understood. In this study we investigated the influence of losartan, an AT1 receptor blocker, and secreted Ang II (sAng II) on glucose uptake and insulin receptor substrate (IRS-1) levels during cardiomyocyte swelling. H9c2 cells were differentiated to cardiac muscle and the levels of myogenin, Myosin Light Chain (MLC), and membrane AT1 receptors were measured using flow cytometry...
September 7, 2017: Peptides
https://www.readbyqxmd.com/read/28886967/class-i-hdacs-control-a-jip1-dependent-pathway-for-kinesin-microtubule-binding-in-cardiomyocytes
#6
Weston W Blakeslee, Ying-Hsi Lin, Matthew S Stratton, Philip D Tatman, Tianjing Hu, Bradley S Ferguson, Timothy A McKinsey
Class I histone deacetylase (HDAC) inhibitors block hypertrophy and fibrosis of the heart by suppressing pathological signaling and gene expression programs in cardiac myocytes and fibroblasts. The impact of HDAC inhibition in unstressed cardiac cells remains poorly understood. Here, we demonstrate that treatment of cultured cardiomyocytes with small molecule HDAC inhibitors leads to dramatic induction of c-Jun amino-terminal kinase (JNK)-interacting protein-1 (JIP1) mRNA and protein expression. In contrast to prior findings, elevated levels of endogenous JIP1 in cardiomyocytes failed to significantly alter JNK signaling or cardiomyocyte hypertrophy...
September 5, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28885685/gut-microbiota-derived-endotoxin-enhanced-the-incidence-of-cardia-bifida-during-cardiogenesis
#7
Jing Zhang, Guang Wang, Jia Liu, Lin-Rui Gao, Meng Liu, Chao-Jie Wang, Manli Chuai, Yongping Bao, Ge Li, Rui-Man Li, Yu Zhang, Xuesong Yang
Background Cytotoxicity and inflammation-associated toxic responses could be induced by bacterial lipopolysaccharides (LPS) in vitro and in vivo respectively. However, the mechanism involved in LPS-induced cardiac malformation in prenatal fetus is still unknown. Methods and results In this study, we demonstrated that LPS was induced in gut microbiota imbalance mice, and next, LPS exposure during gastrulation in the chick embryo increased the incidence of cardia bifida. Gene transfection and tissue transplantation trajectory indicated that LPS exposure restricted the cell migration of cardiac progenitors to primary heart field in gastrula chick embryos...
September 8, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28882480/short-term-administration-of-nicotinamide-mononucleotide-preserves-cardiac-mitochondrial-homeostasis-and-prevents-heart-failure
#8
Rongli Zhang, Yuyan Shen, Lin Zhou, Panjamaporn Sangwung, Hisashi Fujioka, Lilei Zhang, Xudong Liao
Heart failure is associated with mitochondrial dysfunction so that restoring or improving mitochondrial health is of therapeutic importance. Recently, reduction in NAD(+) levels and NAD(+)-mediated deacetylase activity has been recognized as negative regulators of mitochondrial function. Using a cardiac specific KLF4 deficient mouse line that is sensitive to stress, we found mitochondrial protein hyperacetylation coupled with reduced Sirt3 and NAD(+) levels in the heart before stress, suggesting that the KLF4-deficient heart is predisposed to NAD(+)-associated defects...
September 5, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28875579/a-cardiac-patch-from-aligned-microvessel-and-cardiomyocyte-patches
#9
Jeremy A Schaefer, Pilar A Guzman, Sonja B Riemenschneider, Timothy J Kamp, Robert T Tranquillo
Cardiac tissue engineering aims to produce replacement tissue patches in the lab to replace or treat infarcted myocardium. However, current patches lack pre-formed microvascularization and are therefore limited in thickness and force production. In the present study, we sought to assess whether a bi-layer patch composed of a layer made from human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and a microvessel layer composed of self-assembled human blood outgrowth endothelial cells (BOECs) and pericytes (PCs) was capable of engrafting on the epicardial surface of a nude rat infarct model and becoming perfused by the host four weeks after acute implantation...
September 5, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28871207/optical-flow-based-non-invasive-analysis-of-cardiomyocyte-contractility
#10
Andras Czirok, Dona Greta Isai, Edina Kosa, Sheeja Rajasingh, William Kinsey, Zoltan Neufeld, Johnson Rajasingh
Characterization of cardiomyocyte beat patterns is needed for quality control of cells intended for surgical injection as well as to establish phenotypes in disease modeling or toxicity studies. Optical-flow based analysis of videomicroscopic recordings offer a manipulation-free and efficient characterization of contractile cycles, an important characteristics of cardiomyocyte phenotype. We demonstrate that by appropriate computational analysis of optical flow data one can identify distinct contractile centers and distinguish active cell contractility from passive elastic tissue deformations...
September 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28868595/heat-deactivation-of-the-stonefish-synanceia-horrida-venom-implications-for-first-aid-management
#11
Samantha Barnett, Silvia Saggiomo, Michael Smout, Jamie Seymour
OBJECTIVES: To investigate the effects of temperature and hot water immersion time on neutralising venom lethality of the Australian estuarine stonefish (Synanceia horrida). DESIGN: Depths of the spines were measured while venom was extracted from S. horrida individuals. The venom was then exposed to temperatures of 4°C, 37.0°C, 40.1°C, 42.3°C, 45.0°C, 47.7°C, 55.2°C, and 60.0°C for either five or 20 minutes incubation periods. Venom samples were added to cultured human cardiomyocytes and cell viability curves were produced using the ACEA's xCELLigence real-time cell monitoring system...
September 2017: Diving and Hyperbaric Medicine: the Journal of the South Pacific Underwater Medicine Society
https://www.readbyqxmd.com/read/28864889/methylglyoxal-derived-advanced-glycation-end-products-contribute-to-negative-cardiac-remodeling-and-dysfunction-post-myocardial-infarction
#12
Nick J R Blackburn, Branka Vulesevic, Brian McNeill, Cagla Eren Cimenci, Ali Ahmadi, Mayte Gonzalez-Gomez, Aleksandra Ostojic, Zhiyuan Zhong, Michael Brownlee, Paul J Beisswenger, Ross W Milne, Erik J Suuronen
Advanced glycation end-products (AGEs) have been associated with poorer outcomes after myocardial infarction (MI), and linked with heart failure. Methylglyoxal (MG) is considered the most important AGE precursor, but its role in MI is unknown. In this study, we investigated the involvement of MG-derived AGEs (MG-AGEs) in MI using transgenic mice that over-express the MG-metabolizing enzyme glyoxalase-1 (GLO1). MI was induced in GLO1 mice and wild-type (WT) littermates. At 6 h post-MI, mass spectrometry revealed that MG-H1 (a principal MG-AGE) was increased in the hearts of WT mice, and immunohistochemistry demonstrated that this persisted for 4 weeks...
September 1, 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/28859292/exosomes-secreted-by-cardiomyocytes-subjected-to-ischaemia-promote-cardiac-angiogenesis
#13
Teresa M Ribeiro-Rodrigues, Tiago L Laundos, Rita Pereira-Carvalho, Daniela Batista-Almeida, Ricardo Pereira, Vanessa Coelho-Santos, Ana P Silva, Rosa Fernandes, Monica Zuzarte, Francisco J Enguita, Marina C Costa, Perpetua Pinto-do-Ó, Marta T Pinto, Pedro Gouveia, Lino Ferreira, Justin C Mason, Paulo Pereira, Brenda R Kwak, Diana S Nascimento, Henrique Girão
Aims: Myocardial infarction (MI) is the leading cause of morbidity and mortality worldwide and results from an obstruction in the blood supply to a region of the heart. In an attempt to replenish oxygen and nutrients to the deprived area, affected cells release signals to promote the development of new vessels and confer protection against MI. However, the mechanisms underlying the growth of new vessels in an ischaemic scenario remain poorly understood. Here, we show that cardiomyocytes subjected to ischaemia release exosomes that elicit an angiogenic response of endothelial cells (ECs)...
September 1, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28859128/plasma-microvesicle-analysis-identifies-microrna-129-5p-as-a-biomarker-of-heart-failure-in-univentricular-heart-disease
#14
Sweta Ramachandran, Alexander Lowenthal, Carissa Ritner, Shiri Lowenthal, Harold S Bernstein
Biomarkers of heart failure in adults have been extensively studied. However, biomarkers to monitor the progression of heart failure in children with univentricular physiology are less well understood. We proposed that as mediators of diverse pathophysiology, miRNAs contained within circulating microvesicles could serve as biomarkers for the presence and progression of heart failure in univentricular patients. To test this, we studied the association of heart failure with elevations in specific miRNAs isolated from circulating microvesicles in a cohort of children with univentricular heart disease and heart failure...
2017: PloS One
https://www.readbyqxmd.com/read/28857948/suppression-of-oxidative-stress-and-apoptosis-in-electrically-stimulated-neonatal-rat-cardiomyocytes-by-resveratrol-and-underlying-mechanisms
#15
Liqi Ge, Zhirong Wang, Chengzong Li, Yao Zhang, Lei Chen
PURPOSE: We explored effects of resveratrol on oxidative stress in cardiomyocytes subjected to rapid electrical stimulation (RES) and also investigated underlying mechanisms. METHODS: Neonatal rat ventricular myocytes cultured were subjected to RES at 4.0 Hz, with or without resveratrol, an NADPH oxidase inhibitor apocyanin (APO) or a Ca/calmodulin dependent protein kinase II (CaMKII) inhibitor autocamtide-2-inhibitory peptide (AIP). Cell counts, to optimize resveratrol concentration, and angiotensin II content were evaluated...
August 18, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28842809/%C3%AE-catenin-stabilization-promotes-proliferation-and-increase-in-cardiomyocyte-number-in-chick-embryonic-epicardial-explant-culture
#16
Anisha Polley, Puja Sen, Arunima Sengupta, Santanu Chakraborty
Cardiomyocyte (CM) differentiation from proepicardial organ- (PEO) and embryonic epicardium (eEpi)-derived cells or EPDCs in a developing heart emerges as a wide interest in purview of cardiac repair and regenerative medicine. eEpi originates from the precursor PEO and EPDCs, which contribute to several cardiac cell types including smooth muscle cells, fibroblasts, endothelial cells, and CMs during cardiogenesis. Here in this report, we have analyzed several cardiac lineage-specific marker gene expressions between PEO and eEpi cells...
August 25, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28840975/cardioprotection-mediated-by-exosomes-is-impaired-in-the-setting-of-type-ii-diabetes-but-can-be-rescued-by-the-use-of-non-diabetic-exosomes-in-vitro
#17
Sean M Davidson, Jaime A Riquelme, Kaloyan Takov, Jose M Vicencio, Claire Boi-Doku, Vanessa Khoo, Christian Doreth, Dina Radenkovic, Sergio Lavandero, Derek M Yellon
Many patients with ischaemic heart disease also have diabetes. As myocardial infarction is a major cause of mortality and morbidity in these patients, treatments that increase cell survival in response to ischaemia and reperfusion are needed. Exosomes-nano-sized, lipid vesicles released from cells-can protect the hearts of non-diabetic rats. We previously showed that exosomal HSP70 activates a cardioprotective signalling pathway in cardiomyocytes culminating in ERK1/2 and HSP27 phosphorylation. Here, we investigated whether the exosomal cardioprotective pathway remains intact in the setting of type II diabetes...
August 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28838336/effect-of-cdc42-on-myocardial-ischemia-reperfusion-of-rats
#18
X Xu, L Kong, X Song, Z Hao, F Yuan
To investigate the effects and their possible mechanisms of cell division cycle 42 (Cdc42) to neonatal rat myocardial cells subjected to the ischemia-repefusion. Neonatal rat cardiomyocytes were cultured and then subjected to the ischemia-reperfusion. Experimental groups 1. Control group; 2. Ischemia-repefusion group (I/R group); 3. Oligofectamine group (Oli group); 4. Oligofectamine and antisense oligodeoxynucleotide (AS-ODN) group (As group); 5. Oligofectamine and missense oligodeoxynucleotide (MS-ODN) group (Ms group); 6...
August 15, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28837356/phenotypic-assays-for-characterizing-compound-effects-on-induced-pluripotent-stem-cell-derived-cardiac-spheroids
#19
Oksana Sirenko, Michael K Hancock, Carole Crittenden, Matthew Hammer, Sean Keating, Coby B Carlson, Grischa Chandy
Development of more complex, biologically relevant, and predictive cell-based assays for compound screening is a major challenge in drug discovery. The focus of this study was to establish high-throughput compatible three-dimensional (3D) cardiotoxicity assays using human induced pluripotent stem cell-derived cardiomyocytes. Using both high-content imaging and fast kinetic fluorescence imaging, the impact of various compounds on the beating rates and patterns of cardiac spheroids was monitored by changes in intracellular Ca(2+) levels with calcium-sensitive dyes...
August 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/28836732/concise-review-mesenchymal-stem-cells-in-cardiovascular-regeneration-emerging-research-directions-and-clinical-applications
#20
REVIEW
Marcin Majka, Maciej Sułkowski, Bogna Badyra, Piotr Musiałek
Experimental and early clinical data suggest that, due to several unique properties, mesenchymal stem cells (MSCs) may be more effective than other cell types for diseases that are difficult to treat or untreatable. Owing to their ease of isolation and culture as well as their secretory and immunomodulatory abilities, MSCs are the most promising option in the field of cell-based therapies. Although MSCs from various sources share several common characteristics, they also exhibit several important differences...
August 24, 2017: Stem Cells Translational Medicine
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