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https://www.readbyqxmd.com/read/28131956/evaluation-of-the-biofilm-forming-ability-and-its-associated-genes-in-staphylococcus-species-isolates-from-bovine-mastitis-in-argentinean-dairy-farms
#1
Verónica Felipe, Carolina A Morgante, Paola S Somale, Florencia Varroni, María L Zingaretti, Romina A Bachetti, Silvia G Correa, Carina Porporatto
Staphylococcus aureus and coagulase-negative staphylococci (CNS) are important causes of intramammary infection in dairy cattle, and their ability to produce biofilm is considered an important virulence property in the pathogenesis of mastitis. However, the published date on mechanisms and factors involved in infection persistence in the mammary gland remains unclear. The aim of this study was to investigate whether the main Staphylococcus species involved in bovine intramammary infections possess specific characteristics that promote colonization of the udder...
January 25, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28047586/su-g-201-10-experimental-determination-of-modified-tg-43-dosimetry-parameters-for-the-xoft-axxent%C3%A2-electronic-brachytherapy-source
#2
S Simiele, B Palmer, L DeWerd
PURPOSE: The establishment of an air kerma rate standard at NIST for the Xoft Axxent(®) electronic brachytherapy source (Axxent(®) source) motivated the establishment of a modified TG-43 dosimetry formalism. This work measures the modified dosimetry parameters for the Axxent(®) source in the absence of a treatment applicator for implementation in Xoft's treatment planning system. METHODS: The dose-rate conversion coefficient (DRCC), radial dose function (RDF) values, and polar anisotropy (PA) were measured using TLD-100 microcubes with NIST-calibrated sources...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28047086/su-g-tep2-02-characterization-of-an-improved-x-ray-source-for-use-with-the-xoft-surface-applicator
#3
L Kelley, R Neimeyer, T Rusch
PURPOSE: To measure the depth dose distribution and half value layers of an updated x-ray source using the Xoft Axxent Surface Applicator. METHODS: Modifications were made to the existing Axxent HDR X-ray source to increase the dose rate and improve the source life time. Measurements were made comparing dose distributions of this improved x-ray source and the existing Axxent HDR X-ray source. All sources were spatially characterized in a water tank prior to testing and each source passed all acceptance testing prior to measurement...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/27913076/biofilm-production-and-beta-lactamic-resistance-in-brazilian-staphylococcus-aureus-isolates-from-bovine-mastitis
#4
Viviane Figueira Marques, Cássia Couto da Motta, Bianca da Silva Soares, Dayanne Araújo de Melo, Shana de Mattos de Oliveira Coelho, Irene da Silva Coelho, Helene Santos Barbosa, Miliane Moreira Soares de Souza
Staphylococcus spp. play an important role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most relevant species due to the production of virulence factors such as slime, which is required for biofilm formation. This study aimed to evaluate biofilm production and its possible relation to beta-lactamic resistance in 20 S. aureus isolates from bovine mastitic milk. The isolates were characterized by pheno-genotypic and MALDI TOF-MS assays and tested for genes such as icaA, icaD, bap, agr RNAIII, agr I, agr II, agr III, and agr IV, which are related to slime production and its regulation...
January 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27902784/dynamic-in-vivo-mutations-within-the-ica-operon-during-persistence-of-staphylococcus-aureus-in-the-airways-of-cystic-fibrosis-patients
#5
Bianca Schwartbeck, Johannes Birtel, Janina Treffon, Lars Langhanki, Alexander Mellmann, Devika Kale, Janina Kahl, Nina Hirschhausen, Claudia Neumann, Jean C Lee, Friedrich Götz, Holger Rohde, Hanae Henke, Peter Küster, Georg Peters, Barbara C Kahl
Cystic fibrosis (CF) is associated with chronic bacterial airway infections leading to lung insufficiency and decreased life expectancy. Staphylococcus aureus is one of the most prevalent pathogens isolated from the airways of CF patients. Mucoid colony morphology has been described for Pseudomonas aeruginosa, the most common pathogen in CF, but not for S. aureus. From the airways of 8 of 313 CF patients (2.5%) mucoid S. aureus isolates (n = 115) were cultured with a mean persistence of 29 months (range 1 month, 126 months)...
November 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27889140/genotyping-of-methicillin-resistant-staphylococcus-aureus-mrsa-isolated-from-milk-and-dairy-products-in-south-italy
#6
M G Basanisi, G La Bella, G Nobili, I Franconieri, G La Salandra
Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen emerging in hospitals as well as community and livestock. MRSA is a significant and costly public health concern because it may enter the human food chain and contaminate milk and dairy products causing foodborne illness. This study aimed to determine the occurrence and the characteristics of MRSA isolated from 3760 samples of milk and dairy products in a previous survey conducted in southern Italy during 2008-2014. Overall out of 484 S. aureus strains isolated, 40 (8...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27865056/the-caspase-activated-dnase-apoptosis-and-beyond
#7
REVIEW
Brian D Larsen, Claus S Sørensen
Organismal development and function requires multiple and accurate signal transduction pathways to ensure that proper balance between cell proliferation, differentiation, inactivation, and death is achieved. Cell death via apoptotic caspase signal transduction is extensively characterized and integral to this balance. Importantly, the view of apoptotic signal transduction has expanded over the previous decades. Subapoptotic caspase signaling has surfaced as mechanism that can promote the adoption of a range of cellular fates...
November 19, 2016: FEBS Journal
https://www.readbyqxmd.com/read/27852529/seasonal-variation-in-cervical-artery-dissection-in-the-hunter-new-england-region-new-south-wales-australia-a-retrospective-cohort-study
#8
Lucy C Thomas, Andrew P Makaroff, Chris Oldmeadow, John R Attia, Christopher R Levi
BACKGROUND: Cervical artery dissection (CAD) is a leading cause of ischemic stroke among middle aged adults, yet the aetiology remains poorly understood. There are reports from colder northern hemisphere sites of a seasonal pattern in the incidence of CAD. Seasonality may suggest some transient putative pro-inflammatory mechanism but it is unknown whether this also exists in temperate climates. AIMS: To investigate the seasonal variation in incidence of CAD in the xx Region, Australia, and to compare seasonal incidence and selected clinical features between cases of carotid and vertebral artery dissection...
October 20, 2016: Manual Therapy
https://www.readbyqxmd.com/read/27796447/endovascular-treatment-of-acute-internal-carotid-artery-dissections-technical-considerations-clinical-and-angiographic-outcome
#9
Wiebke Kurre, Kai Bansemir, Marta Aguilar Pérez, Rosa Martinez Moreno, Elisabeth Schmid, Hansjörg Bäzner, Hans Henkes
INTRODUCTION: In acute internal carotid artery dissection (a-ICAD) with concomitant intracranial large vessel occlusion or haemodynamic impairment, the effectiveness of medical treatment is limited and endovascular therapy (EVT) can be considered. Feasibility, safety and outcome of EVT in a-ICAD are not well described yet. METHODS: From an institutional database, we retrospectively selected consecutive patients treated for a-ICAD between January 2007 and July 2015...
December 2016: Neuroradiology
https://www.readbyqxmd.com/read/27761465/in-vitro-and-in-vivo-biofilm-characterization-of-methicillin-resistant-staphylococcus-aureus-from-patients-associated-with-pharyngitis-infection
#10
Shanmugaraj Gowrishankar, Arumugam Kamaladevi, Krishnaswamy Balamurugan, Shunmugiah Karutha Pandian
The present investigation was deliberately aimed at evaluating the biofilm-forming ability of 63 clinical MRSA isolates recovered from pharyngitis patients through different phenotypic assays. The molecular detection of adhesion (icaA/icaD/icaB/icaC), adhesins (fnbA/fnbB, clfA, and cna), staphylococcal accessory regulator (sarA), and α-toxin (hla) genes was done by employing polymerase chain reaction (PCR). Out of 63 isolates, 49 (77.8%) were found slime positive by the Congo red agar (CRA) method and 44 (69...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27755284/physical-activity-and-pediatric-obesity-a-quantile-regression-analysis
#11
Jonathan A Mitchell, Marsha Dowda, Russell R Pate, Katarzyna Kordas, Karsten Froberg, Luís B Sardinha, Elin Kolle, Angela Page
PURPOSE: We aimed to determine whether moderate to vigorous physical activity (MVPA) and sedentary behavior (SB) were independently associated with body mass index (BMI) and waist circumference (WC) in children and adolescents. METHODS: Data from the International Children's Accelerometry Database were used to address our objectives (N = 11,115; 6-18 yr; 51% female). We calculated age- and gender-specific BMI and WC z-scores and used accelerometry to estimate MVPA and total SB...
March 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/27751333/bilateral-spontaneous-internal-carotid-artery-dissection-managed-with-endovascular-stenting-a-case-report
#12
Manoj Kumar Agarwala, Azeez Asad, Naveen Gummadi, Sundar Chidambaram, J Venkateswaralu
Carotid artery dissection (CAD) is a frequent cause of stroke, accounting for up to 25% of all ischemic strokes in young and middle-aged patients.(1,2) It may be traumatic or spontaneous, with multi-factorial etiology. A tear in the arterial wall causes intrusion of blood within its layers, producing intra-luminal stenosis, or aneurysmal dilatation.(3) Thrombo-embolism arising from this anatomic disruption has been postulated as the essential stroke mechanism in CAD.(4) Bilateral internal carotid artery dissection (ICAD) has been rarely reported...
September 2016: Indian Heart Journal
https://www.readbyqxmd.com/read/27716472/efficiency-of-a-cleaning-protocol-for-the-removal-of-enterotoxigenic-staphylococcus-aureus-strains-in-dairy-plants
#13
José Guilherme Prado Martin, Gabriela de Oliveira E Silva, Carolina Rodrigues da Fonseca, Caio Baptista Morales, Caroline Souza Pamplona Silva, Daniel Lima Miquelluti, Ernani Porto
Staphylococci are considered a major concern in dairy plants mainly due to the intensive production flow, automation of processing plants and increased demand in the microbiological quality of dairy products. This study aimed to identify S. aureus strains isolated from three Brazilian dairy plants, evaluate the influence of time, temperature and contact surface on the bacterial adhesion process, as well as the efficiency of simulated hygiene and sanitation protocol in removing adhered cells. For genotypic analyses, the presence of icaA and icaD in strains was evaluated...
December 5, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27699055/a-rare-presentation-of-spontaneous-internal-carotid-artery-dissection-with-horner-s-syndrome-viith-xth-and-xiith-nerve-palsies
#14
Azer Majeed, Nuno Pedro Lobato Ribeiro, Asem Ali, Mohsen Hijazi, Hina Farook
Spontaneous internal carotid artery dissection (sICAD) is an uncommon cause of isolated cranial nerve palsies. Commonly patients present with stroke, headache, facial pain and Horner's syndrome, with upto 16% having cranial nerve palsies. We present the case of a 55-year-old man who presented with hoarseness, dysphagia and tongue swelling, mimicking a tongue base tumor. He was found to have unilateral VIIth, Xth and XIIth nerve palsies with Horner's syndrome. Magnetic resonance imaging showed high signal changes and loss of signal void in right internal carotid artery, later confirmed by Angiography as a dissection with pseudo-aneurysm...
October 2016: Oxford Medical Case Reports
https://www.readbyqxmd.com/read/27694725/biofilm-forming-and-antimicrobial-resistance-traits-of-staphylococci-isolated-from-goat-dairy-plants
#15
Myrella Cariry Lira, Paricia Emília Naves Givisiez, Francisca Geovânia Canafístula De Sousa, Marciane Magnani, Evandro Leite De Souza, Denis Augusto Spricigo, Wondwossen Abebe Gebreyes, Celso José Bruno De Oliveira
INTRODUCTION: Biofilm-associated antimicrobial resistance is of increasing importance to the maintenance and spread of foodborne pathogens in the food industry. This study aimed to investigate the ability to form biofilm and the antimicrobial resistance of staphylococci contaminating small-scale goat milk dairy plants. METHODOLOGY: Sixty isolates were tested for antimicrobial resistance against 20 drugs by the microdilution method. Biofilm-forming traits were assessed by the microtiter plate method (MtP), Congo red agar method (CRA), and icaD gene detection by polymerase chain reaction (PCR)...
September 30, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/27677627/spontaneous-and-unruptured-chronic-intracranial-artery-dissection-high-resolution-magnetic-resonance-imaging-findings
#16
Seung Chai Jung, Ho Sung Kim, Choong-Gon Choi, Sang Joon Kim, Sun U Kwon, Dong-Wha Kang, Jong S Kim
PURPOSE: The aim of this article is to present high-resolution magnetic resonance imaging (HR-MRI) findings of chronic stage spontaneous and unruptured intracranial artery dissection (ICAD). MATERIAL AND METHODS: From March 2012 to April 2016 a total of 29 patients (15 male and14 female, age range 37-68 years) with chronic stage spontaneous and unruptured ICAD (vertebral artery 27, posterior inferior cerebellar artery 1 and middle cerebral artery 1) were retrospectively enrolled...
September 27, 2016: Clinical Neuroradiology
https://www.readbyqxmd.com/read/27620826/is-staphylococci-population-from-milk-of-healthy-goats-safe
#17
Patricia Ruiz, Iris Barragán, Susana Seseña, María Llanos Palop
The aim of this study was to assess the species and the genetic diversity of the staphylococci population in raw milk from healthy goats. Isolates representative of all genotypes were screened for their potential pathogenicity by the occurrence of some relevant safety-related properties, such as antibiotic resistance, presence of virulence factor genes, biofilm formation ability and biogenic amine production. A total of 314 staphylococci were isolated, and randomly amplified polymorphic DNA-PCR analysis displayed 48 genotypes...
December 5, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27596733/marein-protects-against-methylglyoxal-induced-apoptosis-by-activating-the-ampk-pathway-in-pc12-cells
#18
Baoping Jiang, Liang Le, Haibo Liu, Lijia Xu, Chunnian He, Keping Hu, Yong Peng, Peigen Xiao
Diabetic encephalopathy, which is characterized by cognitive decline and dementia, commonly occurs in patients with long-standing diabetes. Previous studies have suggested that methylglyoxal (MG), an endogenous toxic compound, plays an important role in diabetic complications such as cognitive impairment. MG induces neuronal apoptosis. To clarify whether marein, a major compound from the hypoglycemic plant Coreopsis tinctoria, prevents PC12 cell damage induced by MG, we cultured PC12 cells in the presence of MG and marein...
2016: Free Radical Research
https://www.readbyqxmd.com/read/27592437/investigation-of-the-antibiotic-resistance-and-biofilm-forming-ability-of-staphylococcus-aureus-from-subclinical-bovine-mastitis-cases
#19
Özkan Aslantaş, Cemil Demir
A total of 112 Staphylococcus aureus isolates obtained from subclinical bovine mastitis cases were examined for antibiotic susceptibility and biofilm-forming ability as well as genes responsible for antibiotic resistance, biofilm-forming ability, and adhesin. Antimicrobial susceptibility of the isolates were determined by disk diffusion method. Biofilm forming ability of the isolates were investigated by Congo red agar method, standard tube method, and microplate method. The genes responsible for antibiotic resistance, biofilm-forming ability, and adhesion were examined by PCR...
November 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27580333/genes-encoding-adhesion-factors-and-biofilm-formation-in-methicillin-resistant-staphylococcus-aureus-in-morocco
#20
Bahija Serray, Salwa Oufrid, Imane Hannaoui, Fatna Bourjilate, Nabila Soraa, Mostafa Mliji, Mohammed Sobh, Abderrahmane Hammoumi, Mohammed Timinouni, Mohamed El Azhari
INTRODUCTION: Infections involving methicillin-resistant Staphylococcus aureus (MRSA) remain a serious threat to hospitalized patients worldwide. MRSA is characterized by recalcitrance to antimicrobial therapy, which is a function not only of widespread antimicrobial resistance, but also the capacity to form biofilms. The present study evaluated the presence of genes encoding adhesion factors and the biofilm-forming capacity in MRSA. METHODOLOGY: In this study, 53 isolates of MRSA, recovered from December 2010 to May 2014 in a mother and child hospital, CHU Mohamed VI in Marrakech, Morocco, were screened for the presence of bap and ica genes associated with biofilm formation, and for bbp, cna, ebpS, eno, fib, fnbA, fnbB, clfA, and clfB genes that encode microbial surface components recognizing adhesive matrix molecules (MSCRAMMs)...
August 31, 2016: Journal of Infection in Developing Countries
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