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https://www.readbyqxmd.com/read/28411078/performance-of-the-xpert-%C3%A2-carba-r-v2-in-the-daily-workflow-of-a-hygiene-unit-in-a-country-with-a-low-prevalence-of-carbapenemase-producing-enterobacteriaceae
#1
Yannick Hoyos, Souad Ouzani, Laurent Dortet, Nicolas Fortineau, Thierry Naas
Early detection of patients colonised with carbapenemase-producing Enterobacteriaceae (CPE) is crucial for implementing proper infection control measures. Here we evaluated the biological performance of the Xpert(®) Carba-R v2 (Cepheid) in the daily workflow of a hygiene unit in a country with a low CPE prevalence. Patients repatriated from countries known for high CPE prevalence or contact patients of a known CPE carrier were targeted as being 'high-risk patients' for CPE carriage. Between September 2015 and March 2016, 241 'high-risk patients' for CPE carriage were screened using the Xpert(®) Carba-R v2 and by plating on chromID(®) CARBA Smart medium (bioMérieux) with and without ertapenem-containing enrichment culture for 24 h...
April 12, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28398123/heterologous-complementation-reveals-a-specialized-activity-for-baca-in-the-medicago-sinorhizobium-meliloti-symbiosis
#2
George C diCenzo, Maryam Zamani, Hannah N Ludwig, Turlough M Finan
The bacterium Sinorhizobium meliloti Rm2011 forms N2-fixing root nodules on alfalfa and other leguminous plants. The pSymB chromid contains a 110-kb region (the ETR region) showing high synteny to a chromosomally located region in Sinorhizobium fredii NGR234 and related rhizobia. We recently introduced the ETR region from S. fredii NGR234 into the S. meliloti chromosome. Here, we report that, unexpectedly, the S. fredii NGR234 ETR region did not complement deletion of the S. meliloti ETR region in symbiosis with Medicago sativa...
April 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28389190/detection-of-vancomycin-resistant-enterococci-vre-in-stool-specimens-submitted-for-clostridium-difficile-toxin-testing
#3
Sevim Özsoy, Arzu İlki
The aim of this study was to determine the association between Clostridium difficile (C. difficile) and vancomycin-resistant Enterococcus (VRE) and efficacy of screening stools submitted for C. difficile toxin assay for prevalence of VRE. Between April 2012 and February 2014, 158 stool samples submitted for C. difficile toxin to the Marmara University Microbiology Laboratory, were included in the study. Stool samples were analyzed by enzyme immuno assay test; VIDAS (bioMerieux, France) for Toxin A&B. Samples were inoculated on chromID VRE (bioMerieux, France) and incubated 24h at 37°C...
March 17, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28378973/tawny-owl-strix-aluco-as-a-potential-transmitter-of-enterobacteriaceae-epidemiologically-relevant-for-forest-service-workers-nature-protection-service-and-ornithologists
#4
Grzegorz Grzywaczewski, Danuta Kowalczyk-Pecka, Szymon Cios, Wiktor Bojar, Andrzej Jankuszew, Hubert Bojar, Marcin Kolejko
Established taxa within the Enterobacteriaceae wereisolated from cloacal swabs of Strix aluco chicks in nest boxes located at five research sites. ChromID ESBL medium (bioMerieux) was used to select a pool of Enterobacteriaceae strains producing extended-spectrum beta-lactamases. Drug sensitivity of the chosen strains was determined from the full pool of Enterobacteriaceae to 6 chemotherapeutics of different mechanisms of action. The study evaluated the sensitivity of ESBL-synthesizing isolates to substances belonging to penicillins, cephalosporins, cephamycins, clavams, carbapenems and monobactams...
March 31, 2017: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/28340557/development-of-selective-medium-for-imp-type-carbapenemase-producing-enterobacteriaceae-in-stool-specimens
#5
Norihisa Yamamoto, Ryuji Kawahara, Yukihiro Akeda, Rathina Kumar Shanmugakani, Hisao Yoshida, Hideharu Hagiya, Naohiro Hara, Isao Nishi, Satomi Yukawa, Rumiko Asada, Yumi Sasaki, Kazuhiro Maeda, Noriko Sakamoto, Shigeyuki Hamada, Kazunori Tomono
BACKGROUND: Identification of carbapenemase-producing Enterobacteriaceae (CPE) in faecal specimens is challenging. This fact is particularly critical because low-level carbapenem-resistant organisms such as IMP-producing CPE are most prevalent in Japan. We developed a modified selective medium more suitable for IMP-type CPE. METHODS: Fifteen reference CPE strains producing different types of β-lactamases were used to evaluate the commercially available CHROMagar KPC and chromID CARBA as well as the newly prepared MC-ECC medium (CHROMagar ECC supplemented with meropenem, cloxacillin, and ZnSO4) and M-ECC medium (CHROMagar ECC supplemented with meropenem and ZnSO4)...
March 24, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28283520/biogeography-of-a-novel-clade-of-ensifer-meliloti-associated-with-the-australian-legume-trigonella-suavissima
#6
Bertrand Eardly, Patrick Elia, John Brockwell, Daniel Golemboski, Peter van Berkum
Here we describe a novel clade within Ensifer meliloti and consider how geographic and ecological isolation contributed to the limited distribution of this group. Members of the genus Ensifer are best known for their ability to form nitrogen-fixing symbioses with forage legumes of three related genera, Medicago L., Melilotus Mill., and Trigonella L., which are members of the tribe Trifoleae. They have a natural distribution extending from the Mediterranean basin through west Asia, where there is an unsurpassed number of species belonging to these genera...
March 10, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28267783/fecal-carriage-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-and-klebsiella-pneumoniae-after-urinary-tract-infection-a-three-year-prospective-cohort-study
#7
Silje B Jørgensen, Arne Søraas, Arnfinn Sundsfjord, Knut Liestøl, Truls M Leegaard, Pål A Jenum
We have performed a prospective cohort study to investigate the duration of and risk factors for prolonged fecal carriage of ESBL-producing Escherichia coli or Klebsiella pneumoniae in patients with community acquired urinary tract infection caused by these bacteria. From 2009 to 2011, 101 Norwegian patients were recruited. Stool swabs and questionnaires were collected every three months for one year and at the end of the study in 2012. Information on antibiotic prescriptions was collected from the Norwegian Prescription Database...
2017: PloS One
https://www.readbyqxmd.com/read/28264559/a-family-of-single-copy-repabc-type-shuttle-vectors-stably-maintained-in-the-alpha-proteobacterium-sinorhizobium-meliloti
#8
Johannes Döhlemann, Marcel Wagner, Carina Happel, Martina Carrillo, Patrick Sobetzko, Tobias J Erb, Martin Thanbichler, Anke Becker
A considerable share of bacterial species maintains segmented genomes. Plant symbiotic α-proteobacterial rhizobia contain up to six repABC-type replicons in addition to the primary chromosome. These low or unit-copy replicons, classified as secondary chromosomes, chromids, or megaplasmids, are exclusively found in α-proteobacteria. Replication and faithful partitioning of these replicons to the daughter cells is mediated by the repABC region. The importance of α-rhizobial symbiotic nitrogen fixation for sustainable agriculture and Agrobacterium-mediated plant transformation as a tool in plant sciences has increasingly moved biological engineering of these organisms into focus...
March 20, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28131671/comparison-of-two-rapid-biochemical-tests-and-four-chromogenic-selective-media-for-detection-of-carbapenemase-producing-gram-negative-bacteria
#9
Vladimira Hinić, Ivo Amrein, Sabrina Stammler, Judith Heckendorn, Dominik Meinel, Reno Frei, Adrian Egli
We evaluated RAPIDEC® CARBA NP, Neo-Rapid CARB, chromID® CARBA SMART (CARB/OXA), Brilliance™ CRE/ESBL, ChromArt CRE and BBL™ CHROMagar™ CPE for the detection of carbapenemase-producing bacteria. The analytical sensitivity of RAPIDEC® CARBA NP was better than that of Neo-Rapid CARB. A combination of carbapenemase and ESBL screening plates could be advantageous.
January 25, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28119780/prevalence-and-molecular-epidemiology-of-clostridium-difficile-infection-in-thailand
#10
P Putsathit, M Maneerattanaporn, P Piewngam, P Kiratisin, T V Riley
Little is known about Clostridium difficile infection (CDI) in Asia generally, and specifically in Thailand. Given the high prevalence of inappropriate antibiotic usage in this region, CDI is likely to be common. This study investigated the prevalence and molecular epidemiology of CDI in Thailand. Stool specimens collected from inpatients with diarrhoea at Siriraj hospital in Bangkok (n = 422) were cultured on ChromID Cdiff agar and any presumptive C. difficile colonies were identified, PCR ribotyped and toxin profiled...
January 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/28108388/c-%C3%A2-difficile-pcr-ribotype-023-might-go-undetected-when-using-chromid-c-%C3%A2-difficile-agar
#11
COMPARATIVE STUDY
E Reigadas, L Alcalá, M Marín, A Martín, E Bouza
We compared the performance of the new chromogenic medium ChromID C. difficile with that of CLO agar. ChromID C. difficile agar is a sensitive medium that can accelerate the presumptive identification of C. difficile, however ribotype 023 might go undetected when using this chromogenic medium.
April 2017: Anaerobe
https://www.readbyqxmd.com/read/28073895/evaluation-of-genotypic-and-phenotypic-methods-to-detect-carbapenemase-production-in-gram-negative-bacilli
#12
Allison R McMullen, Melanie L Yarbrough, Meghan A Wallace, Angela Shupe, Carey-Ann D Burnham
BACKGROUND: Carbapenemase-producing gram-negative bacteria (CP-GNB) are an urgent and expanding public health threat. Rapid and accurate identification of these organisms facilitates infection prevention efforts in healthcare facilities. The objective of our study was to evaluate methods to detect and identify CP-GNB. METHODS: We examined 189 carbapenem-resistant GNB(CR-GNB), including Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii complex, using 3 different methods: 2 methods to screen isolates of GNB for carbapenemase production [the carbapenem inactivation method (CIM) and 2 chromogenic agars] and a molecular method (Cepheid GeneXpert Carba-R) to identify the mechanism of carbapenem resistance and the associated resistance genes (blaKPC, blaNDM, blaIMP, blaOXA-48-like, and blaVIM)...
March 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28056800/networking-in-microbes-conjugative-elements-and-plasmids-in-the-genus-alteromonas
#13
Mario López-Pérez, Nieves Ramon-Marco, Francisco Rodriguez-Valera
BACKGROUND: To develop evolutionary models for the free living bacterium Alteromonas the genome sequences of isolates of the genus have been extensively analyzed. However, the main genetic exchange drivers in these microbes, conjugative elements (CEs), have not been considered in detail thus far. In this work, CEs have been searched in several complete Alteromonas genomes and their sequence studied to understand their role in the evolution of this genus. Six genomes are reported here for the first time...
January 5, 2017: BMC Genomics
https://www.readbyqxmd.com/read/27936054/high-prevalence-of-faecal-carriage-of-esbl-producing-enterobacteriaceae-among-children-in-dar-es-salaam-tanzania
#14
Marit G Tellevik, Bjørn Blomberg, Øyvind Kommedal, Samuel Y Maselle, Nina Langeland, Sabrina J Moyo
BACKGROUND: Faecal carriage of ESBL-producing bacteria is a potential risk for transmission and infection. Little is known about faecal carriage of antibiotic resistance in Tanzania. This study aimed to investigate the prevalence of faecal carriage of ESBL-producing Enterobacteriaceae and to identify risk factors for carriage among young children in Tanzania. METHODOLOGY/PRINCIPAL FINDINGS: From August 2010 to July 2011, children below 2 years of age were recruited in Dar es Salaam, including healthy community children (n = 250) and children hospitalized due to diarrhoea (n = 250) or other diseases (n = 103)...
2016: PloS One
https://www.readbyqxmd.com/read/27760718/laboratory-detection-of-intestinal-carriage-of-carbapenemase-producing-enterobacteriaceae-a-comparison-of-algorithms-using-the-carba-np-test
#15
COMPARATIVE STUDY
James Knox, Claire Gregory, Louise Prendergast, Chandrika Perera, Jennifer Robson, Lynette Waring
Stool specimens spiked with a panel of 46 carbapenemase-producing Enterobacteriaceae (CPE) and 59 non-carbapenemase producers were used to compare the diagnostic accuracy of 4 testing algorithms for the detection of intestinal carriage of CPE: (1) culture on Brilliance ESBL agar followed by the Carba NP test; (2) Brilliance ESBL followed by the Carba NP test, plus chromID OXA-48 agar with no Carba NP test; (3) chromID CARBA agar followed by the Carba NP test; (4) chromID CARBA followed by the Carba NP test, plus chromID OXA-48 with no Carba NP test...
January 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27625418/evaluation-of-the-performance-of-c-diff-quik-chek-complete-and-its-usefulness-in-a-hospital-setting-with-a-high-prevalence-of-clostridium-difficile-infection
#16
Hae-Sun Chung, Miae Lee
Rapid and accurate diagnosis of Clostridium difficile infection (CDI) is crucial for patient care, infection control, and efficient surveillance. We evaluated C. DIFF QUIK CHEK COMPLETE (QCC; TechLab), which detects glutamate dehydrogenase (GDH) antigen (QCC-Ag) and toxin A/B (QCC-Tox) simultaneously, and compared it to the laboratory diagnostics for CDI currently in use in a tertiary hospital setting with a high prevalence of CDI. QCC, RIDASCREEN C. difficile toxin A/B assay (Toxin EIA; R-Biopharm AG), chromID C...
January 2017: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
https://www.readbyqxmd.com/read/27582518/culture-of-urine-specimens-by-use-of-chromid-cps-elite-medium-can-expedite-escherichia-coli-identification-and-reduce-hands-on-time-in-the-clinical-laboratory
#17
Melanie L Yarbrough, Meghan A Wallace, Cynthia Marshall, Erin Mathias, C A Burnham
Urine is one of the most common specimen types submitted to the clinical microbiology laboratory; the use of chromogenic agar is one method by which the laboratory might expedite culture results and reduce hands-on time and materials required for urine culture analysis. The objective of our study was to compare chromID CPS Elite (bioMérieux), a chromogenic medium, to conventional primary culture medium for evaluation of urine specimens. Remnant urine specimens (n = 200) were inoculated into conventional media and into chromID CPS Elite agar (chromID)...
November 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27562853/chromogenic-media-for-mrsa-diagnostics
#18
REVIEW
Zhenbo Xu, Yuchao Hou, Brian M Peters, Dingqiang Chen, Bing Li, Lin Li, Mark E Shirtliff
For the past decade, a number of chromogenic media for methicillin-resistance Staphylococcus aureus (MRSA) detection have been developed and applied, including Oxoid Brilliance™ MRSA, CHROMagar™ MRSA, BBL™ CHROMagar™ MRSA, MRSASelect and chromID MRSA. The advantages of these chromogenic media offers direct detection of visible staphylococcal colonies, coupled with the use of chromogenic enzymatic substrates that can be hydrolyzed by S. aureus to confirm species or strain identification. BBL™ CHROMagar™ MRSA and MRSASelect are designed for detection of nasal colonization by MRSA, while CHROMagar™ MRSA, Oxoid Brilliance™ MRSA and chromID MRSA are readily applied in bacterial screening...
November 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27556286/performance-of-the-artus-c-difficile-qs-rgq-kit-for-the-detection-of-toxigenic-clostridium-difficile
#19
Hee-Won Moon, Hyeong Nyeon Kim, Ji-Young Kim, Mina Hur, Heejung Kim, Yeo-Min Yun
OBJECTIVES: Nucleic acid amplification tests are commonly used for the direct detection of toxigenic Clostridium difficile. We evaluated the diagnostic performance of newly launched, artus C. difficile QS-RGQ Kit (artus C. difficile, QIAGEN, Hilden, Germany), in comparison with toxigenic culture (TC) and Xpert C. difficile (Cepheid, Sunnyvale, CA, USA). DESIGN AND METHODS: In prospectively collected 261 diarrheal specimens, the artus C. difficile and the Xpert C...
January 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/27534258/performance-of-the-chromid-salmonella-elite-chromogenic-agar-in-comparison-with-chromagar%C3%A2-salmonella-oxoid%C3%A2-brilliance%C3%A2-salmonella-and-hektoen-agars-for-the-isolation-of-salmonella-from-stool-specimens
#20
COMPARATIVE STUDY
Delphine Martiny, Anne Dediste, Claire Anglade, Linda Vlaes, Catherine Moens, Souad Mohamed, Olivier Vandenberg
chromID™ Salmonella Elite is compared with 3 culture media commonly used for Salmonella isolation from stool specimens. As results were equivalent to other chromogenic media (100% sensitivity, 98% specificity), only financial arguments should guide the choice for a medium with respect to another.
October 2016: Diagnostic Microbiology and Infectious Disease
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