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https://www.readbyqxmd.com/read/29156756/mir-221-regulated-kit-level-by-wild-type-or-leukemia-mutant-runx1-a-determinant-of-single-myeloblast-fate-decisions-that-collectively-drives-or-hinders-granulopoiesis
#1
Stefano Rossetti, Michael J Anauo, Nicoletta Sacchi
RUNX1, a master transcription factor of hematopoiesis, was shown to orchestrate both cell proliferation and differentiation during granulopoiesis by regulating microRNAs (miRs). In this study, taking advantage of the miR-ON reporter system, we monitored first, how the granulocyte colony stimulation factor (GCSF) temporally modulates the concomitant level variation of miR-221 and one of its prototypic targets, the stem cell factor receptor KIT, in single 32D(miR-ON-221) myeloblasts expressing wild type RUNX1...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156635/is-school-type-associated-with-objectively-measured-physical-activity-in-15-year-olds
#2
Lovro Štefan, Maroje Sorić, Antonela Devrnja, Hrvoje Podnar, Marjeta Mišigoj-Duraković
The main aims of this study were: (1) to determine the objectively assessed physical activity (PA) patterns in urban 15-year-old male and female adolescents according to school type and (2) to assess the differences in PA between school days and weekend days. In this cross-sectional study, participants were 187 secondary-school male and female adolescents (61.4% females) attending grammar and vocational schools. Patterns of PA were objectively evaluated using a multi-sensor body monitor for 5 consecutive days...
November 20, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29156123/a-nanopore-based-label-free-and-real-time-monitoring-assay-for-dna-methyltransferase-activity-and-inhibition
#3
Sana Rauf, Ling Zhang, Asghar Ali, Jalal Ahmad, Yang Liu, Jinghong Li
DNA methylation catalyzed by DNA methyltransferase plays an important role in many biological processes. However, conventional assays proposed for DNA methyltransferase activity are laborious and discontinuous. We describe a novel method for real-time monitoring of the activity and kinetics of Escherichia coli DNA adenine methyltransferase (Dam), using nanopore technique coupled with enzyme-linkage reactions. A double-stranded DNA probe AB having a recognition sequence 5'-GATC-3' for both Dam and MboI restriction endonuclease was prepared...
November 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29155720/m%C3%A3-ller-glia-cell-activation-in-a-laser-induced-retinal-degeneration-and-regeneration-model-in-zebrafish
#4
Federica M Conedera, Petra Arendt, Carolyn Trepp, Markus Tschopp, Volker Enzmann
A fascinating difference between teleost and mammals is the lifelong potential of the teleost retina for retinal neurogenesis and regeneration after severe damage. Investigating the regeneration pathways in zebrafish might bring new insights to develop innovative strategies for the treatment of retinal degenerative diseases in mammals. Herein, we focused on the induction of a focal lesion to the outer retina in adult zebrafish by means of a 532 nm diode laser. A localized injury allows investigating biological processes that take place during retinal degeneration and regeneration directly at the area of damage...
October 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155562/quantification-of-nanomaterial-nanomedicine-trafficking-in-vivo
#5
Liming Wang, Liang Yan, Jing Liu, Chunying Chen, Yuliang Zhao
Absorption, distribution, metabolism and excretion (ADME) of nanomaterials (NMs) or nanomedicines (NMs), are fundamental biological processes that dominate their nanomedical functions or nanotoxicities. Mostly, NMs exist in biological systems as a form of nanoparticles (NPs). However, to determine the composition, spatial distribution, and states of existence of NMs/NPs in vivo, upon entry into the body, as well as to monitor the dynamic changes of these information over time remain a challenge for the current analytical methodology due to their limited efficiently in characterizing interactions between these complex(nano)-complex(bio) systems...
November 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29155255/the-role-of-epigenetics-in-cardiovascular-health-and-aging-a-focus-on-physical-activity-and-nutrition
#6
REVIEW
Robert G Wallace, Laura C Twomey, Marc-Antoine Custaud, Jonathan Turner, Niall Moyna, Philip M Cummins, Ronan P Murphy
The cardiovascular system is responsible for transport of blood and nutrients to tissues, and is pivotal to the physiological health and longevity. Epigenetic modification is a natural, age-associated process resulting in highly contextualised gene expression with clear implications for cell differentiation and disease onset. Biological/epigenetic age is independent of chronological age, constituting a highly reflective snapshot of an individual's overall health. Accelerated vascular aging is of major concern, effectively lowering disease threshold...
November 16, 2017: Mechanisms of Ageing and Development
https://www.readbyqxmd.com/read/29154986/gv1001-induces-apoptosis-by-reducing-angiogenesis-in-rcc-cells-both-in-vitro-and-in-vivo
#7
Ga Eun Kim, Ae Ryang Jung, Mee Young Kim, Joseph Bada Lee, Ji Houn Im, Kyu Won Lee, Yong Hyun Park, Ji Youl Lee
Objective To investigate the anti-cancer effects of GV1001 and its biological mechanism of action in renal cell carcinoma (RCC). Methods The effects of GV1001 on cell survival and apoptosis in RCC cells were examined in vitro using cell viability assay, fluorescence-activated cell sorting (FACS), and Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. To evaluate the effect of GV1001 on migration, invasion and angiogenesis, we used wound healing, invasion, endothelial cell tube formation assay and western blot analysis...
November 15, 2017: Urology
https://www.readbyqxmd.com/read/29154875/optimization-of-pegylation-reaction-time-and-molar-ratio-of-rhg-csf-toward-increasing-bioactive-potency-of-monopegylated-protein
#8
Jamila Behi, RymHassiki, Nadia Ben Said, Balkiss Bouhaouala-Zahar, Mohamed Lamine Benkhoud
PEGylation is one of the strategies used for enhancing in vivo residence time of recombinant human Granulocyte Colony-Stimulating Factor (rhG-CSF) and therefore reducing in dose frequency to better fit with patient comfort treatment. In this study, three methoxy polyethylene glycol propionaldehydes (mPEG- ALD) of 10, 20 and 30kDa MW were utilized to produce biologically active monoPEGylated rhG-CSF with enhanced molecular weight. PEGylation reactions were carried out at room temperature and pH 5.0 in the presence of cyanoborohydride and two mPEG-ALD: protein molar ratios (3:1 and 5:1)...
November 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29153877/slowly-biodegradable-organic-compounds-impact-the-biostability-of-non-chlorinated-drinking-water-produced-from-surface-water
#9
W A M Hijnen, R Schurer, J A Bahlman, H A M Ketelaars, R Italiaander, A van der Wal, P W J J van der Wielen
It is possible to distribute drinking water without a disinfectant residual when the treated water is biologically stable. The objective of this study was to determine the impact of easily and slowly biodegradable compounds on the biostability of the drinking water at three full-scale production plants which use the same surface water, and on the regrowth conditions in the related distribution systems. Easily biodegradable compounds in the drinking water were determined with AOC-P17/Nox during 2012-2015. Slowly biodegradable organic compounds measured as particulate and/or high-molecular organic carbon (PHMOC), were monitored at the inlet and after the different treatment stages of the three treatments during the same period...
November 6, 2017: Water Research
https://www.readbyqxmd.com/read/29153726/bioindicators-for-monitoring-marine-litter-ingestion-and-its-impacts-on-mediterranean-biodiversity
#10
REVIEW
Maria Cristina Fossi, Cristina Pedà, Montserrat Compa, Catherine Tsangaris, Carme Alomar, Francoise Claro, Christos Ioakeimidis, Francois Galgani, Tatjana Hema, Salud Deudero, Teresa Romeo, Pietro Battaglia, Franco Andaloro, Ilaria Caliani, Silvia Casini, Cristina Panti, Matteo Baini
The Mediterranean Sea has been described as one of the most affected areas by marine litter in the world. Although effects on organisms from marine plastic litter ingestion have been investigated in several oceanic areas, there is still a lack of information from the Mediterranean Sea. The main objectives of this paper are to review current knowledge on the impact of marine litter on Mediterranean biodiversity, to define selection criteria for choosing marine organisms suitable for use as bioindicator species, and to propose a methodological approach to assessing the harm related to marine litter ingestion in several Mediterranean habitats and sub-regions...
November 16, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29153172/a-100-year-review-metabolic-health-indicators-and-management-of-dairy-cattle
#11
T R Overton, J A A McArt, D V Nydam
Our aim in this Journal of Dairy Science centennial review is to describe the evolution of focus on metabolic indicators, from discovery and description to evaluation at the individual cow and subsequently herd levels, over the past 100 yr. Furthermore, we discuss current and future technologies that will be used in the dairy industry to utilize these indicators widely going forward. Knowledge of chemical changes in various fluids (e.g., blood, urine, and milk) accompanying numerous metabolic disease states in the dairy cow has existed since almost the beginning of the Journal of Dairy Science 100 yr ago...
December 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29153166/a-100-year-review-practical-female-reproductive-management
#12
J S Stevenson, J H Britt
Basic knowledge of mechanisms controlling reproductive processes in mammals was limited in the early 20th century. Discoveries of physiologic processes and mechanisms made early in the last century laid the foundation to develop technologies and programs used today to manage and control reproduction in dairy cattle. Beyond advances made in understanding of gonadotropic support and control of ovarian and uterine functions in basic reproductive biology, advancements made in artificial insemination (AI) and genetics facilitated rapid genetic progress of economically important traits in dairy cattle...
December 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29152025/advances-in-microfluidic-devices-made-from-thermoplastics-used-in-cell-biology-and-analyses
#13
REVIEW
Elif Gencturk, Senol Mutlu, Kutlu O Ulgen
Silicon and glass were the main fabrication materials of microfluidic devices, however, plastics are on the rise in the past few years. Thermoplastic materials have recently been used to fabricate microfluidic platforms to perform experiments on cellular studies or environmental monitoring, with low cost disposable devices. This review describes the present state of the development and applications of microfluidic systems used in cell biology and analyses since the year 2000. Cultivation, separation/isolation, detection and analysis, and reaction studies are extensively discussed, considering only microorganisms (bacteria, yeast, fungi, zebra fish, etc...
September 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/29151844/pym-a-new-affordable-image-based-method-using-a-raspberry-pi-to-phenotype-plant-leaf-area-in-a-wide-diversity-of-environments
#14
Benoît Valle, Thierry Simonneau, Romain Boulord, Francis Sourd, Thibault Frisson, Maxime Ryckewaert, Philippe Hamard, Nicolas Brichet, Myriam Dauzat, Angélique Christophe
Background: Plant science uses increasing amounts of phenotypic data to unravel the complex interactions between biological systems and their variable environments. Originally, phenotyping approaches were limited by manual, often destructive operations, causing large errors. Plant imaging emerged as a viable alternative allowing non-invasive and automated data acquisition. Several procedures based on image analysis were developed to monitor leaf growth as a major phenotyping target. However, in most proposals, a time-consuming parameterization of the analysis pipeline is required to handle variable conditions between images, particularly in the field due to unstable light and interferences with soil surface or weeds...
2017: Plant Methods
https://www.readbyqxmd.com/read/29150907/sensitivity-of-doping-biomarkers-after-administration-of-a-single-dose-testosterone-gel
#15
Jenny Mullen, Annica Börjesson, Oscar Hopcraft, Jenny J Schulze, Magnus Ericsson, Anders Rane, Mikael Lehtihet, Lena Ekström
Micro-doping with testosterone (T) is challenging to detect with the current doping tests. Today, the methods available to detect T are longitudinally monitoring of urine biomarkers in the Athlete Biological Passport (ABP) and measuring the isotopic composition of excreted biomarkers to distinguish the origin of the molecule. In this study, we investigated the detectability of a single dose of 100 mg T gel in 8 healthy male subjects. We also studied which biomarkers were most sensitive to T gel administration, including blood biomarkers...
November 18, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29150298/spontaneous-osteoclastogenesis-hypothesis-for-gender-unrelated-osteoporosis-screening-and-diagnosis
#16
F Salamanna, R Giardino, M Fini
Women are at greater risk of developing osteoporosis (OP). However, in the past few years it has become more widely recognized that OP is a significant problem also in men although OP is frequently under-diagnosed and, consequently, under treated in men. Most guidelines, screening and fracture risk evaluation methods as well as pharmacologic agents have been developed for women and then adapted to men. Bone Mineral Density (BMD) measurement by Dual X-ray Absorptiometry (DEXA) is reported as T score and the capability of DEXA to diagnose OP and predict fracture risk is still debated...
November 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/29149935/biomarkers-in-sepsis
#17
REVIEW
Tjitske S R van Engelen, Willem Joost Wiersinga, Brendon P Scicluna, Tom van der Poll
A biomarker is a characteristic by which a (patho)physiologic process can be identified. Biomarkers can be of diagnostic value (to discriminate infection from noninfectious conditions or to determine the causative pathogen), of prognostic value (assigning risk profiles and predict outcome), and in the future may be of theranostic value (aid in selection and monitoring of therapy). Systems biology provides a promising tool for the discovery of novel biomarkers. Biomarkers can be the key to personalized targeted treatment in the future clinical management of sepsis...
January 2018: Critical Care Clinics
https://www.readbyqxmd.com/read/29149857/putative-progressive-and-abortive-feline-leukemia-virus-infection-outcomes-in-captive-jaguarundis-puma-yagouaroundi
#18
Claudia Filoni, A Katrin Helfer-Hungerbuehler, José Luiz Catão-Dias, Mara Cristina Marques, Luciana Neves Torres, Manfred Reinacher, Regina Hofmann-Lehmann
BACKGROUND: Feline leukemia virus (FeLV) is an exogenous gammaretrovirus of domestic cats (Felis catus) and some wild felids. The outcomes of FeLV infection in domestic cats vary according to host susceptibility, virus strain, and infectious challenge dose. Jaguarundis (Puma yagouaroundi) are small wild felids from South and Central America. We previously reported on FeLV infections in jaguarundis. We hypothesized here that the outcomes of FeLV infection in P. yagouaroundi mimic those observed in domestic cats...
November 17, 2017: Virology Journal
https://www.readbyqxmd.com/read/29149764/human-saliva-as-a-diagnostic-material
#19
REVIEW
Sylwia Chojnowska, Tomasz Baran, Iwona Wilińska, Paulina Sienicka, Iwona Cabaj-Wiater, Małgorzata Knaś
Today blood biochemical laboratory tests are essential elements to the diagnosis and monitoring of the treatment of diseases. However, many researchers have suggested saliva as an preferable diagnostic material. The collection of saliva is simple, painless, cheap and safe, both for patients and medical staff. An additional advantage of saliva is the fact that it may be retrieved several times a day, which makes repeat analysis much easier. Furthermore, saliva has very high durability. Although 94-99% of salivary content is water, saliva also contains numerous cellular elements and many organic and inorganic substances, including most biological markers present in the blood and urine that may be used in the early detection and monitoring of many dental and general diseases...
November 14, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/29149240/z-scores-based-methods-and-their-application-to-biological-monitoring-an-example-in-professional-soccer-players
#20
Guillaume Saulière, Jérôme Dedecker, Laurie-Anne Marquet, Pierre Rochcongar, Jean-Francois Toussaint, Geoffroy Berthelot
The clinical and biological follow-up of individuals, such as the biological passport for athletes, is typically based on the individual and longitudinal monitoring of hematological or urine markers. These follow-ups aim to identify abnormal behavior by comparing the individual's biological samples to an established baseline. These comparisons may be done via different ways, but each of them requires an appropriate extra population to compute the significance levels, which is a non-trivial issue. Moreover, it is not necessarily relevant to compare the measures of a biomarker of a professional athlete to that of a reference population (even restricted to other athletes), and a reasonable alternative is to detect the abnormal values by considering only the other measurements of the same athlete...
November 15, 2017: Biostatistics
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