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https://www.readbyqxmd.com/read/28433692/enhanced-biological-phosphorus-removal-at-low-sludge-retention-time-in-view-of-its-integration-in-a-stage-systems
#1
Carlos Chan, Albert Guisasola, Juan Antonio Baeza
The two-stage A/B WWTP configuration is being studied as a possible wastewater treatment with low energy consumption or even with a net energy generation. The first phase, A-stage, is designed to remove organic matter at very short Sludge Retention Time (SRT), while the B-stage is based on autotrophic nitrogen removal. However, P-removal in the A/B process usually only relies on precipitation. This work studies the potential inclusion of Enhanced Biological Phosphorus Removal (EBPR) in the A-stage phase. For this aim, the long-term operation of three different Sequencing Batch Reactors (SBR) enriched in Accumulibacter at low SRT was thoroughly monitored for more than three months each one...
April 9, 2017: Water Research
https://www.readbyqxmd.com/read/28433330/bivalent-rlp2086-trumenba%C3%A2-development-of-a-well-characterized-vaccine-through-commercialization
#2
Khurram Sunasara, John Cundy, Sriram Srinivasan, Brad Evans, Weiqiang Sun, Scott Cook, Eric Bortell, John Farley, Daniel Griffin, Michele Bailey Piatchek, Katherine Arch-Douglas
The phrase "Process is the Product" is often applied to biologics, including multicomponent vaccines composed of complex components that evade complete characterization. Vaccine production processes must be defined and locked early in the development cycle to ensure consistent quality of the vaccine throughout scale-up, clinical studies, and commercialization. This approach of front-loading the development work helped facilitate the accelerated approval of the Biologic License Application for the well-characterized vaccine bivalent rLP2086 (Trumenba®, Pfizer Inc) in 2014 under Breakthrough Therapy Designation...
April 19, 2017: Vaccine
https://www.readbyqxmd.com/read/28433060/enhanced-photoresponsive-polyethyleneimine-citric-acid-co-carbonized-dots-for-facile-and-selective-sensing-and-intracellular-imaging-of-cobalt-ions-at-physiologic-ph
#3
Wen-Sheng Zou, Qing-Chun Zhao, Jun Zhang, Xiao-Ming Chen, Xiu-Fang Wang, Lin Zhao, Shao-Hua Chen, Ya-Qin Wang
Whether as an important biological element or as a radioactive source/medicine, the monitoring of trace levels of cobalt ions (Co) has become a non-negligible factor for human health and green environment. Current technologies for the detection of Co are cost-expensive and time-consuming, and require cumbersome sample pretreatment process. Herein a novel sensing platform has been developed for Co detection based on the quenching of the enhanced fluorescence signal of polyamine functionalized C-dots. Amine groups at the surface of the C-dots can capture Zn(2+)/Cd(2+) to form coordination compound, which can inhibit the photoinduced electron transfer pathways of C-dots and then induce the fluorescence enhancement of the C-dots by ∼80% margin...
June 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28432990/influence-of-red-mud-on-soil-microbial-communities-application-and-comprehensive-evaluation-of-the-biolog-ecoplate-approach-as-a-tool-in-soil-microbiological-studies
#4
Viktória Feigl, Éva Ujaczki, Emese Vaszita, Mónika Molnár
Red mud can be applied as soil ameliorant to acidic, sandy and micronutrient deficient soils. There are still knowledge gaps regarding the effects of red mud on the soil microbial community. The Biolog EcoPlate technique is a promising tool for community level physiological profiling. This study presents a detailed evaluation of Biolog EcoPlate data from two case studies. In experiment "A" red mud from Ajka (Hungary) was mixed into acidic sandy soil in soil microcosms at 5-50 w/w%. In experiement "B" red mud soil mixture was mixed into low quality subsoil in a field experiment at 5-50 w/w%...
April 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28432628/design-of-water-quality-monitoring-networks-with-two-information-scenarios-in-tropical-andean-basins
#5
Juan Carlos Bastidas, Jorge Julián Vélez, Jeannette Zambrano, Adela Londoño
Design and redesign of water quality monitoring networks were evaluated for two similarly sized watersheds in the tropical Andes via optimization techniques using geographic information system technology (GIS) and a matter-element analysis of 5-day biological oxygen demand (BOD5) and total suspended solids (TSS). This resulted in a flexible, objectively based design for a 1128-km(2) watershed without prior water quality data (La Miel River), and a network redesign of a 1052-km(2) watershed with historical water quality monitoring (Chinchiná River)...
April 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28432341/detection-of-red-blood-cell-surface-antigens-by-probe-triggered-cell-collision-and-flow-retardation-in-an-autonomous-microfluidic-system
#6
Éva Sautner, Krisztián Papp, Eszter Holczer, Eszter L Tóth, Rita Ungai-Salánki, Bálint Szabó, Péter Fürjes, József Prechl
Microfluidic devices exploit combined physical, chemical and biological phenomena that could be unique in the sub-millimeter dimensions. The current goal of development of Point-of-Care (POC) medical devices is to extract the biomedical information from the blood. We examined the characteristics of blood flow in autonomous microfluidic devices with the aim to realize sensitive detection of interactions between particulate elements of the blood and the appropriately modified surfaces of the system. As a model experiment we demonstrated the fast analysis of the AB0 blood group system...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28431764/seasonal-monitoring-of-blue-mussel-mytilus-spp-populations-in-a-harbor-area-a-focus-on-responses-to-environmental-factors-and-chronic-contamination
#7
C Lacroix, E Duvieilbourg, N Guillou, J Guyomarch, C Bassoulet, D Moraga, G Chapalain, M Auffret
Coastal waters corresponding to macrotidal systems are among the most variable marine biotopes. Sessile animals as bivalve mollusks may however be found forming intertidal beds at high densities, as allowed by full adaptation to local conditions. A better knowledge of adaptive responses to environmental factors is required to foresee possible adverse effects of global change. At the sub-cellular level, transcriptional responses are among the earliest signals of environmental disturbances and they can reveal subtle and meaningful changes in organism exposed to stress...
April 12, 2017: Marine Environmental Research
https://www.readbyqxmd.com/read/28431363/nanomaterial-based-electrochemical-sensors-for-arsenic-a-review
#8
REVIEW
Sureshkumar Kempahanumakkagari, Akash Deep, Ki-Hyun Kim, Suresh Kumar Kailasa, Hye-On Yoon
The existence of arsenic in the environment poses severe global health threats. Considering its toxicity, the sensing of arsenic is extremely important. Due to the complexity of environmental and biological samples, many of the available detection methods for arsenic have serious limitations on selectivity and sensitivity. To improve sensitivity and selectivity and to circumvent interferences, different electrode systems have been developed based on surface modification with nanomaterials including carbonaceous nanomaterials, metallic nanoparticles (MNPs), metal nanotubes (MNTs), and even enzymes...
April 13, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28431313/raman-spectroscopy-for-the-characterization-of-different-fractions-of-hemp-essential-oil-extracted-at-130%C3%A2-c-using-steam-distillation-method
#9
Muhammad Asif Hanif, Haq Nawaz, Saima Naz, Rubina Mukhtar, Nosheen Rashid, Ijaz Ahmad Bhatti, Muhammad Saleem
In this study, Raman spectroscopy along with Principal Component Analysis (PCA) is used for the characterization of pure essential oil (pure EO) isolated from the leaves of the Hemp (Cannabis sativa L.,) as well as its different fractions obtained by fractional distillation process. Raman spectra of pure Hemp essential oil and its different fractions show characteristic key bands of main volatile terpenes and terpenoids, which significantly differentiate them from each other. These bands provide information about the chemical composition of sample under investigation and hence can be used as Raman spectral markers for the qualitative monitoring of the pure EO and different fractions containing different active compounds...
April 1, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28428191/the-role-of-minimal-residual-disease-testing-in-myeloma-treatment-selection-and-drug-development-current-value-and-future-applications
#10
Kenneth C Anderson, Daniel Auclair, Gary J Kelloff, Caroline C Sigman, Herve Avet-Loiseau, Ann T Farrell, Nicole J Gormley, Shaji K Kumar, Ola Landgren, Nikhil C Munshi, Michele Cavo, Faith E Davies, Alessandra Di Bacco, Jennifer S Dickey, Steven I Gutman, Howard R Higley, Mohamad A Hussein, J Milburn Jessup, Ilan R Kirsch, Richard F Little, Robert D Loberg, Jens G Lohr, Lata Mukundan, James L Omel, Trevor J Pugh, Gregory H Reaman, Michael D Robbins, A Kate Sasser, Nancy Valente, Elena Zamagni
Treatment of myeloma has benefited from the introduction of more effective and better tolerated agents, improvements in supportive care, better understanding of disease biology, revision of diagnostic criteria, and new sensitive and specific tools for disease prognostication and management. Assessment of minimal residual disease (MRD) in response to therapy is one of these tools, as longer progression-free survival (PFS) is seen consistently among patients who have achieved MRD negativity. Current therapies lead to unprecedented frequency and depth of response, and next generation flow and sequencing methods to measure MRD in bone marrow are in use and being developed with sensitivities in the range of 10(-5)‒10(-6) cells...
April 20, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28427292/chemical-analyses-and-in-vitro-and-in-vivo-toxicity-of-fruit-methanol-extract-of-sechium-edule-var-nigrum-spinosum
#11
Itzen Aguiñiga-Sánchez, Jorge Cadena-Íñiguez, Edelmiro Santiago-Osorio, Guadalupe Gómez-García, Víctor Manuel Mendoza-Núñez, Juana Rosado-Pérez, Mirna Ruíz-Ramos, Víctor Manuel Cisneros-Solano, Edgar Ledesma-Martínez, Angel de Jesus Delgado-Bordonave, Ramón Marcos Soto-Hernández
CONTEXT: Sechium edule (Jacq.) Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor cell lines. OBJECTIVE: This study investigates the chemical composition and biological activities of extracts obtained from S. edule var. nigrum spinosum. MATERIALS AND METHODS: The leukemia P388 cell line and mononuclear bone marrow cells (MNCBMs) were treated with the extract at a concentration ranging from 40 to 2370 μg/mL for cytotoxicity and viability assays...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28426437/supersonic-transient-magnetic-resonance-elastography-for-quantitative-assessment-of-tissue-elasticity
#12
Yu Liu, Jingfei Liu, Brett Z Fite, Josquin Foiret, Asaf Ilovitsh, J Kent Leach, Erik Dumont, Charles F Caskey, Katherine W Ferrara
Non-invasive, quantitative methods to assess the properties of biological tissues are needed for many therapeutic and tissue engineering applications. Magnetic resonance elastography (MRE) has historically relied on external vibration to generate periodic shear waves. In order to focally assess a biomaterial or to monitor the response to ablative therapy, the interrogation of a specific region of interest by a focused beam is desirable and transient MRE (t-MRE) techniques have previously been developed to accomplish this goal...
April 20, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28425787/defining-the-proteomic-landscape-of-rheumatoid-arthritis-progress-and-prospective-clinical-applications
#13
Lucía Lourido, Francisco J Blanco, Cristina Ruiz-Romero
The heterogeneity of Rheumatoid Arthritis (RA) and the absence of clinical tests accurate enough to identify the early stages of this disease have hampered its management. Therefore, proteomics research is increasingly focused on the discovery of novel biological markers, which would not only be able make an early diagnosis, but also to gain insight into the different pathological mechanisms underlying the heterogeneity of RA and also to stratify patients, which is critical to enabling effective treatments...
April 20, 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/28425543/in-situ-nuclear-magnetic-resonance-microimaging-of-live-biofilms-in-a-microchannel
#14
R S Renslow, M J Marshall, A E Tucker, W B Chrisler, X-Y Yu
Biofilms are comprised of microbial cells and an extracellular polymeric substance (EPS) matrix that supports interactions between community members and with the local environment. The highly hydrated EPS matrix makes the application of many biofilm visualization techniques difficult. Hence, to better visualize how biofilms interact with their environment, there is a need for imaging techniques to monitor hydrated state biofilm dynamics. We employed an in situ dynamic approach to construct label-free images of biofilms...
April 20, 2017: Analyst
https://www.readbyqxmd.com/read/28424742/fret-from-single-to-multiplexed-signaling-events
#15
REVIEW
Gertrude Bunt, Fred S Wouters
Förster resonance energy transfer (FRET) is a powerful tool for the visualization of molecular signaling events such as protein activities and interactions in cells. In its different implementations, FRET microscopy has been mainly used for monitoring single events. Recently, there has been a trend of extending FRET imaging towards the simultaneous detection of multiple events and interactions. The concomitant increase in experimental complexity requires a deeper understanding of the biophysical background of FRET...
April 2017: Biophysical Reviews
https://www.readbyqxmd.com/read/28424669/inferring-microbial-interactions-in-the-gut-of-the-hong-kong-whipping-frog-polypedates-megacephalus-and-a-validation-using-probiotics
#16
Francis Cheng-Hsuan Weng, Grace Tzun-Wen Shaw, Chieh-Yin Weng, Yi-Ju Yang, Daryi Wang
The concerted activity of intestinal microbes is crucial to the health and development of their host organisms. Investigation of microbial interactions in the gut should deepen our understanding of how these micro-ecosystems function. Due to advances in Next Generation Sequencing (NGS) technologies, various bioinformatic strategies have been proposed to investigate these microbial interactions. However, due to the complexity of the intestinal microbial community and difficulties in monitoring their interactions, at present there is a gap between the theory and biological application...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28424339/discovery-of-double-stranded-genomic-dna-in-circulating-exosomes
#17
Raghu Kalluri, Valerie S LeBleu
It is becoming increasingly clear that small vesicles released from cells (extracellular vesicles [EVs]) represent a heterogeneous population implicated in cell-to-cell communication. The classifications and nomenclature of EVs are evolving as enrichment strategies and specific characteristics are being unraveled. At present, physical properties of EVs-namely, size, shape, and density-are often used to identify subpopulations of EVs. A distinct group of EVs, termed exosomes, largely defined by their small size (∼40-150 nm) and proposed subcellular origin, has been extensively studied in several aspects of cancer biology...
April 19, 2017: Cold Spring Harbor Symposia on Quantitative Biology
https://www.readbyqxmd.com/read/28424107/early-maturation-and-substance-use-across-adolescence-and-young-adulthood-a-longitudinal-study-of-finnish-twins
#18
Jeanne E Savage, Richard J Rose, Lea Pulkkinen, Karri Silventoinen, Tellervo Korhonen, Jaakko Kaprio, Nathan Gillespie, Danielle M Dick
Early maturation, indexed by pubertal development (PD), has been associated with earlier initiation and greater frequency of adolescent substance use, but this relationship may be biased by confounding factors and effects that change across development. Using a population-based Finnish twin sample (N = 3,632 individuals), we conducted twin modeling and multilevel structural equation modeling of the relationship between PD and substance use at ages 12-22. Shared environmental factors contributed to early PD and heavier substance use for females...
April 20, 2017: Development and Psychopathology
https://www.readbyqxmd.com/read/28421220/a-highly-selective-colorimetric-and-environment-sensitive-optical-potassium-ion-sensor
#19
Guangjie Song, Ruofan Sun, Jiqing Du, Meiwan Chen, Yanqing Tian
Potassium ions (K(+)) play vital roles in many biological processes and thus highly selective sensors for K(+) are critical for disease diagnosis and health monitoring. Herein, we report a colorimetric K(+) sensor (KS7) in which a hemicyanine dye was used as a fluorophore and phenylaza-[18]crown-6 lariat ether (ACLE) was utilized as a K(+) ligand. The maximum absorption peak of KS7 shifted hypsochromically by 77 nm (from 515 to 438 nm) with an isosbestic point at 452 nm upon the addition of K(+) to its aqueous solution accompanied by a color change from red to yellow...
April 19, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28420793/regulating-plant-physiology-with-organic-electronics
#20
David J Poxson, Michal Karady, Roger Gabrielsson, Aziz Y Alkattan, Anna Gustavsson, Siamsa M Doyle, Stéphanie Robert, Karin Ljung, Markus Grebe, Daniel T Simon, Magnus Berggren
The organic electronic ion pump (OEIP) provides flow-free and accurate delivery of small signaling compounds at high spatiotemporal resolution. To date, the application of OEIPs has been limited to delivery of nonaromatic molecules to mammalian systems, particularly for neuroscience applications. However, many long-standing questions in plant biology remain unanswered due to a lack of technology that precisely delivers plant hormones, based on cyclic alkanes or aromatic structures, to regulate plant physiology...
April 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
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