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https://www.readbyqxmd.com/read/29661068/correlation-between-radiological-parameters-and-functional-outcomes-in-patients-older-than-60-years-of-age-with-distal-radius-fracture
#1
Rodrigo Gutiérrez-Monclus, Héctor Gutiérrez-Espinoza, Jonathan Zavala-González, Cristian Olguín-Huerta, David Rubio-Oyarzún, Felipe Araya-Quintanilla
BACKGROUND: The relationship between radiographic and functional outcomes is controversial in the elderly. The objective of this study is to determine whether there is a correlation between functional outcome and acceptable distal radius fracture (DRF) alignment in patients older than 60 years of age. METHODS: This correlation study was carried out at the Central Metropolitan Health Service of Chile. A total of 180 patients diagnosed with extra-articular DRF, according to the AO classification, were prospectively recruited...
April 1, 2018: Hand: Official Journal of the American Association for Hand Surgery
https://www.readbyqxmd.com/read/29610276/the-transcriptional-regulator-ccctc-binding-factor-limits-oxidative-stress-in-endothelial-cells
#2
Anna R Roy, Abdalla Ahmed, Peter V DiStefano, Lijun Chi, Nadiya Khyzha, Niels Galjart, Michael D Wilson, Jason E Fish, Paul Delgado Olguin
The CCCTC-binding factor (CTCF) is a versatile transcriptional regulator required for embryogenesis, but its function in vascular development or in diseases with a vascular component is poorly understood. Here, we found that endothelial Ctcf is essential for mouse vascular development and limits accumulation of reactive oxygen species (ROS). Conditional knockout of Ctcf in endothelial progenitors and their descendants affected embryonic growth, and caused lethality at embryonic day 10.5 owing to defective yolk sac and placental vascular development...
April 2, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29584529/characterization-and-pharmacokinetics-of-triamcinolone-acetonide-loaded-liposomes-topical-formulations-for-vitreoretinal-drug-delivery
#3
Juan C Altamirano-Vallejo, Jose Navarro-Partida, Alejandro Gonzalez-De la Rosa, Jane H Hsiao, Jose S Olguín-Gutierrez, Ana C Gonzalez-Villegas, Brian C Keller, Lourdes Bouzo-Lopez, Arturo Santos
PURPOSE: To achieve a safer alternative to intravitreal injection of corticosteroids, we developed and characterized triamcinolone acetonide-loaded liposomes formulations (TA-LFs) to be used topically for vitreoretinal drug delivery. METHODS: Four different 0.2% TA-LFs (TA-LF1 to TA-LF4) were generated and submitted to physicochemical characterization. Posteriorly, an ex vivo diffusion assay was performed using rabbit corneas as membranes. Finally, concentrations of triamcinolone acetonide (TA) were determined by high-performance liquid chromatography in ocular tissues from New Zealand white rabbits after multiple topical doses of TA-LF2 (6 times per day, 14 days)...
March 27, 2018: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29565169/biochemical-and-histological-changes-produced-by-sweeteners-and-cytarabine-in-the-brain-of-young-rats
#4
Ernestina Hernández García, Norma Osnaya Brizuela, Armando Valenzuela Peraza, David Calderón Guzmán, Maribel Ortiz Herrera, Hugo Juárez Olguín, Gerardo Barragán Mejía, Daniel Santamaría Del Ángel, Alberto Rojas Ochoa
OBJECTIVE: The aim of this study was to evaluate the effect of splenda and stevia on dopamine and 5-HIAA levels, and some biomarkers of oxidative stress in the presence of cytarabine. METHODS: Forty-eight young male Wistar rats each with a weight of 80 g (four weeks of age), distributed in six groups of eight animals each, were treated as follows: group 1, control (NaCl 0.9% vehicle); group 2, cytarabine (0.6 g/kg); group 3, stevia (0.6 g/kg); group 4, cytarabine + stevia; group 5, splenda; and group 6, cytarabine + splenda...
February 13, 2018: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/29564766/isolation-and-culture-of-mouse-placental-endothelial-cells
#5
Lijun Chi, Paul Delgado-Olguin
Isolation and culture of endothelial cells (ECs) is a useful tool to study the cellular processes involved in vascular development and vascular maturation. In this chapter, we describe a method to isolate and culture endothelial cells from placentae. This method takes advantage of two transgenes: ROSA26mT/mG , which drives the expression of GFP upon Cre-mediated recombination, and Tie2-Cre, which expresses Cre driven by the Tie2 promoter in endothelial progenitors and their descendants. GFP-expressing endothelial cells are isolated through fluorescence-activated cell sorting (FACS)...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29564765/isolating-embryonic-cardiac-progenitors-and-cardiac-myocytes-by-fluorescence-activated-cell-sorting
#6
Abdalla Ahmed, Paul Delgado-Olguin
Isolation of highly purified populations of embryonic cardiomyocytes enables the study of congenital cardiac phenotypes at the cellular level. Fluorescent-activated cell sorting (FACS) is normally used to isolate fluorescently tagged cells. Here we describe the isolation of differentiating mouse embryonic cardiac progenitors and cardiomyocytes at embryonic day (E) 9.5 and E13.5, respectively by FACS. Over 50,000 differentiating cardiac progenitors and 200,000 cardiomyocytes can be obtained in a single prep using the methods described...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29564762/live-imaging-of-fetal-intra-abdominal-organs-using-two-photon-laser-scanning-microscopy
#7
Yuhki Koike, Bo Li, Yong Chen, Hiromu Miyake, Carol Lee, Lijun Chi, Richard Wu, Mikihiro Inoue, Keiichi Uchida, Masato Kusunoki, Paul Delgado-Olguin, Agostino Pierro
The processes by which the intra-abdominal organ circulatory system develops in the embryo and during organogenesis are unclear. Previous studies have used fixed tissues to study the development of abdominal organ vasculature in the embryo; however, the intravital circulation of intra-abdominal organs in rodent fetal development has not been studied. This protocol describes a system that uses two-photon laser-scanning microscopy (TPLSM) for real-time observation and quantification of normal and pathologic live fetal intra-abdominal dynamics while the fetus is still connected to the mother via the umbilical cord...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29564758/visualizing-the-vascular-network-in-the-mouse-embryo-and-yolk-sac
#8
Anna R Roy, Paul Delgado-Olguin
Whole mount immunofluorescence is a valuable technique that can be used to visualize vascular networks in early developing embryonic tissues. This technique involves the permeabilization of fixed mouse embryos and yolk sacs, and primary antibody tagging of the endothelial cell marker platelet endothelial cell adhesion molecule 1 (Pecam-1). A secondary antibody tagged with a fluorophore targets the primary antibody, fluorescently labeling endothelial cells and revealing vascular networks.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29564757/mouse-genotyping
#9
Sandra Vuong, Paul Delgado-Olguin
Genotyping is an invaluable tool for identifying organisms carrying genetic variations including insertions and deletions. This method involves the extraction of DNA from animal tissue samples and subsequent amplification of genomic regions of interest by polymerase chain reaction (PCR). The amplified products are analyzed by agarose gel electrophoresis based on their size.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29555646/cell-extrinsic-hematopoietic-impact-of-ezh2-inactivation-in-fetal-liver-endothelial-cells
#10
Wen Hao Neo, Christopher A G Booth, Emanuele Azzoni, Lijun Chi, Paul Delgado-Olguín, Marella F T R de Bruijn, Sten Eirik W Jacobsen, Adam J Mead
Despite the well-established cell-intrinsic role of epigenetic factors in normal and malignant hematopoiesis, their cell-extrinsic role remains largely unexplored. Herein we investigated the hematopoietic impact of inactivating Ezh2 , a key component of polycomb repressive complex 2 (PRC2), in the fetal liver (FL) vascular niche. Hematopoietic specific ( Vav -iCre) Ezh2 -inactivation enhanced FL hematopoietic stem cell (HSC) expansion with normal FL erythropoiesis. In contrast, endothelium ( Tie2 -Cre) targeted Ezh2 -inactivation resulted in embryonic lethality with severe anemia at E13...
March 19, 2018: Blood
https://www.readbyqxmd.com/read/29539615/epidermal-growth-factor-receptor-regulation-of-ewing-sarcoma-cell-function
#11
Nathália Kersting, Bárbara Kunzler Souza, Igor Araujo Vieira, Rafael Pereira Dos Santos, Danielly Brufatto Olguins, Lauro José Gregianin, André Tesainer Brunetto, Algemir Lunardi Brunetto, Rafael Roesler, Caroline Brunetto de Farias, Gilberto Schwartsmann
OBJECTIVE: Ewing sarcoma (ES) is a type of childhood cancer probably arising from stem mesenchymal or neural crest cells. The epidermal growth factor receptor (EGFR) acts as a driver oncogene in many types of solid tumors. However, its involvement in ES remains poorly understood. METHODS: Human SK-ES-1 and RD-ES ES cells were treated with EGF, the EGFR inhibitor tyrphostin (AG1478), or phosphoinositide 3-kinase (PI3K) or extracellular-regulated kinase (ERK)/mitogen-activated kinase (MAPK) inhibitors...
March 14, 2018: Oncology
https://www.readbyqxmd.com/read/29533739/%C3%AE-actin-shows-limited-mobility-and-is-only-required-for-supraphysiological-insulin-stimulated-glucose-transport-in-young-adult-soleus-muscle
#12
Agnete B Madsen, Jonas R Knudsen, Carlos Henriquez-Olguin, Yeliz Angin, Kristien J Zaal, Lykke Sylow, Peter Schjerling, Evelyn Ralston, Thomas E Jensen
Studies in skeletal muscle cell cultures suggest that the cortical actin cytoskeleton is a major requirement for insulin-stimulated glucose transport, implicating the β-actin isoform which, in many cell types, is the main actin isoform. However, it is not clear that β-actin plays such a role in mature skeletal muscle. Neither dependency of glucose transport on β-actin nor actin reorganization upon glucose transport have been tested in mature muscle. To investigate the role of β-actin in fully differentiated muscle, we have performed a detailed characterization of wildtype and muscle-specific β-actin knockout (KO) mice...
March 13, 2018: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29466371/ezh2-is-not-required-for-cardiac-regeneration-in-neonatal-mice
#13
Abdalla Ahmed, Tao Wang, Paul Delgado-Olguin
The neonatal mouse heart has the remarkable capacity to regenerate lost myocardium within the first week of life. Neonatal cardiomyocytes re-express fetal genes that control cell proliferation after injury to promote regeneration. The loss of regenerative capacity of the heart one week after birth coincides with repression of a fetal transcriptional program coordinated by epigenetic regulators. The histone methyltransferase enhancer of zeste homolog 2 (Ezh2) is a repressor of fetal cardiac transcriptional programs and suppresses cardiomyocyte cell proliferation, suggesting a potential function in heart regeneration...
2018: PloS One
https://www.readbyqxmd.com/read/29465957/-effect-of-management-on-tree-diversity-in-secondary-vegetation-in-the-biosphere-reserve-of-calakmul-campeche-mexico
#14
Ana María Báez-Vargas, Ligia Esparza-Olguín, Eduardo Martínez-Romero, Susana Ochoa-Gaona, Neptalí Ramírez-Marcial, Noel Antonio González-Valdivia
The tropical dry forest (BTS) of Yucatan Peninsula has been managed for centuries, but the relationship between these management efforts and their effects on trees diversity has not been fully understood. The aim of this study was to evaluate the effect of forest management (thinning, harvesting and enrichment of species), in the structure of secondary vegetation derived from dry tropical forests, in two communities in Calakmul, Campeche, Southeast Mexico. We analyzed changes in the composition, species richness, species diversity, and structure in secondary vegetation subject to following types of management: (1) secondary vegetation with beekeeping management (MA), secondary vegetation with forest management (MF), natural secondary vegetation (SM) and tropical dry forest (BTS)...
March 2017: Revista de Biología Tropical
https://www.readbyqxmd.com/read/29425778/preface
#15
EDITORIAL
Eugenia J Olguín, Joan García, Jaume Puigagut
No abstract text is available yet for this article.
February 6, 2018: New Biotechnology
https://www.readbyqxmd.com/read/29390337/linezolid-associated-optic-neuropathy-in-a-pediatric-patient-with-mycobacterium-nonchromogenicum-a-case-report
#16
Napoleón González Saldaña, Diego Mauricio Galvis Trujillo, Ana Maria Borbolla Pertierra, Ana Ivette Mondragón Pineda, Hugo Juárez Olguín
BACKGROUND: Toxic optic neuropathies are alterations of the optic nerve and can be caused by environmental, pharmacological, or nutritional agents. CASE: It is about a 7-year-old male patient, a native of the State of Mexico, Mexico who was diagnosed with cervical mycobacterial lymphadenitis that required management with linezolid. OBSERVATIONS: After 7 months of treatment, visual acuity of the left eye decreased and was accompanied by headache...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29344269/early-and-partial-reduction-in-cd4-foxp3-regulatory-t-cells-during-colitis-associated-colon-cancer-induces-cd4-and-cd8-t-cell-activation-inhibiting-tumorigenesis
#17
Jonadab E Olguín, Itzel Medina-Andrade, Emmanuel Molina, Armando Vázquez, Thalia Pacheco-Fernández, Rafael Saavedra, Carlos Pérez-Plasencia, Yolanda I Chirino, Felipe Vaca-Paniagua, Luis E Arias-Romero, Emma B Gutierrez-Cirlos, Sonia A León-Cabrera, Miriam Rodriguez-Sosa, Luis I Terrazas
Colorectal cancer (CRC) is the second most commonly diagnosed cancer in women and the third in men in North America and Europe. CRC is associated with inflammatory responses in which intestinal pathology is caused by different cell populations including a T cell dysregulation that concludes in an imbalance between activated T (Tact) and regulatory T (Treg) cells. Treg cells are CD4+Foxp3+ cells that actively suppress pathological and physiological immune responses, contributing to the maintenance of immune homeostasis...
2018: Journal of Cancer
https://www.readbyqxmd.com/read/29281144/size-growth-and-density-data-for-shallow-water-sea-urchins-from-mexico-to-the-aleutian-islands-alaska-1956-2016
#18
Thomas A Ebert, Louis M Barr, James L Bodkin, Dirk Burcham, Dominique Bureau, Henry S Carson, Nancy L Caruso, Jennifer E Caselle, Jeremy T Claisse, Sabrina Clemente, Kathryn Davis, Paul M Detwiler, John D Dixon, David O Duggins, John M Engle, James A Estes, Scott D Groth, Benjamin M Grupe, Peter Halmay, Kyle P Hebert, José C Hernández, Laura J Jurgens, Peter E Kalvass, Michael C Kenner, Brenda Konar, David J Kushner, Lynn C Lee, David L Leighton, Gabriela Montaño-Moctezuma, J Eric Munk, Irma Olguin, Julio S Palleiro, David O Parker, John S Pearse, Daniel J Pondella, Laura Rogers-Bennett, Stephen C Schroeter, Andrew Olaf Shelton, Jorge Sonnenholzner, Ian K Taniguchi, Glenn R VanBlaricom, Jane C Watson, Benjamin P Weitzman, Jonathan P Williams, Jennifer Yakimishyn, Zane Zhang
Size, growth, and density have been studied for North American Pacific coast sea urchins Strongylocentrotus purpuratus, S. droebachiensis, S. polyacanthus, Mesocentrotus (Strongylocentrotus) franciscanus, Lytechinus pictus, Centrostephanus coronatus, and Arbacia stellata by various workers at diverse sites and for varying lengths of time from 1956 to present. Numerous peer-reviewed publications have used some of these data but some data have appeared only in graduate theses or the gray literature. There also are data that have never appeared outside original data sheets...
March 2018: Ecology
https://www.readbyqxmd.com/read/29226152/the-phytoestrogen-genistein-produces-similar-effects-as-17-%C3%AE-estradiol-on-anxiety-like-behavior-in-rats-at-12-weeks-after-ovariectomy
#19
Juan Francisco Rodríguez-Landa, Jonathan Cueto-Escobedo, Abraham Puga-Olguín, Eduardo Rivadeneyra-Domínguez, Blandina Bernal-Morales, Emma Virginia Herrera-Huerta, Andrea Santos-Torres
The phytoestrogen genistein produces anxiolytic-like effects in ovariectomized rats, which highlights its potential therapeutic effect in ameliorating anxiety in surgical menopausal women. However, no studies have directly compared the effects of identical doses of genistein and 17 β -estradiol, the main estrogen used in hormone replacement therapy in menopausal women. The present study evaluated the anxiolytic-like effects of identical doses of genistein and 17 β -estradiol (0.045, 0.09, and 0.18 mg/kg/7 days, s...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29171626/-exercise-as-a-tool-to-reduce-body-weight
#20
Carlos Burgos, Carlos Henríquez-Olguín, Rodrigo Ramírez-Campillo, Sandra Mahecha Matsudo, Hugo Cerda-Kohler
No abstract text is available yet for this article.
June 2017: Revista Médica de Chile
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