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https://www.readbyqxmd.com/read/29844902/gene-expression-changes-associated-with-chemotherapy-resistance-in-ewing-sarcoma-cells
#1
Leonardo Horbach, Marialva Sinigaglia, Camila Alves Da Silva, Danielly Brufatto Olguins, Lauro José Gregianin, Algemir Lunardi Brunetto, André Tesainer Brunetto, Rafael Roesler, Caroline Brunetto De Farias
Ewing Sarcoma (ES) is a highly aggressive bone and soft tissue childhood cancer. The development of resistance to chemotherapy is common and remains the main cause of treatment failure. We herein evaluated the expression of genes associated with chemotherapy resistance in ES cell lines. A set of genes (CCAR1, TUBA1A, POLDIP2, SMARCA4 and SMARCB1) was data-mined for resistance against doxorubicin and vincristine, which are the standard drugs used in the treatment of patients with ES. The expression of each gene in SK-ES-1 ES cells was reported before and after exposure to a drug resistance-inducing protocol...
June 2018: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/29843731/tyrphostin-ag17-inhibits-adipocyte-differentiation-in-vivo-and-in-vitro
#2
Alberto Camacho, Juan Carlos Segoviano-Ramírez, Adriana Sánchez-Garcia, Jose de Jesus Herrera-de la Rosa, Jaime García-Juarez, Carlos Alberto Hernandez-Puente, Geovana Calvo-Anguiano, Sergio Rodolfo Maltos-Uro, Alejandra Olguin, Gabriel Gojon-Romanillos, Gabriel Gojon-Zorrilla, Rocio Ortiz-Lopez
BACKGROUND: Excessive subcutaneous adiposity in obesity is associated to positive white adipocyte tissue (WAT) differentiation (adipogenesis) and WAT expandability. Here, we hypothesized that supplementation with the insulin inhibitor and mitochondrial uncoupler, Tyrphostin (T-AG17), in vitro and in vivo inhibits adipogenesis and adipocyte hypertrophy. METHODS: We used a 3T3-L1 proadipocyte cell line to identify the potential effect of T-AG17 on adipocyte differentiation and fat accumulation in vitro...
May 29, 2018: Lipids in Health and Disease
https://www.readbyqxmd.com/read/29789313/blast-xyplot-viewer-a-tool-for-performing-blast-in-whole-genome-sequenced-bacteria-archaea-and-visualize-whole-results-simultaneously
#3
Yagul Pedraza-Pérez, Rodrigo Alberto Cuevas-Vede, Ángel Bernardo Canto-Gómez, Liliana López-Pliego, Rosa María Gutiérrez-Ríos, Ismael Hernández-Lucas, Gustavo Rubín-Linares, Ygnacio Martínez-Laguna, Jesús Francisco López-Olguín, Luis Ernesto Fuentes-Ramírez
One of the most commonly used tools to compare protein or DNA sequences against databases is BLAST. We introduce a web tool that allows the performance of BLAST-searches of protein/DNA sequences in whole-genome sequenced bacteria/archaea, and displays a large amount of BLAST-results simultaneously. The circular bacterial replicons are projected as horizontal lines with fixed length of 360, representing the degrees of a circle. A coordinate system is created with length of the replicon along the x -axis and the number of replicon used on the y -axis...
May 22, 2018: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29773670/high-%C3%AE-tocopherol-dosing-increases-lipid-metabolism-by-changing-redox-state-in-damaged-rat-gastric-mucosa-and-liver-after-ethanol-treatment
#4
Marisela Olguín-Martínez, Diego R Hernández-Espinosa, Rolando Hernández-Muñoz
Regeneration of ethanol-injured rat gastric mucosa must undergo changes in major metabolic pathways to achieve DNA replication and cell proliferation. These events are highly dependent on glucose utilization and inhibited by vitamin E (α-tocopherol) administration. Therefore, this study was aimed at assessing lipid metabolism in the gastric mucosa and ethanol-induced gastric damage and the effect of α-tocopherol administration. For this, rates of fatty acid β-oxidation and lipogenesis were tested in gastric mucosa samples...
May 17, 2018: Clinical Science (1979-)
https://www.readbyqxmd.com/read/29744938/accurate-determination-of-energy-requirements-in-hospitalised-patients-with-parenteral-nutrition
#5
G Quiroz-Olguín, I Medina-Vera, A E Serralde-Zúñiga, A Gulias-Herrero, A I Sánchez-Rosales, M Guevara-Cruz
BACKGROUND: The prescription of parenteral nutrition (PN) in hospitalised patients requires an estimation of the energy requirements. Most studies employing prediction equations (PEs) to estimate energy requirements have focused on critically ill patients. The present study aimed to evaluate several PEs of the resting energy expenditure (REE) to identify the most accurate equation for estimating the REE required for PN. METHODS: This cross-sectional and descriptive study included patients hospitalised with medical or surgical diagnoses, making them candidates for PN...
May 9, 2018: Journal of Human Nutrition and Dietetics: the Official Journal of the British Dietetic Association
https://www.readbyqxmd.com/read/29730778/expression-of-genes-associated-with-inflammation-and-iron-metabolism-in-3t3-l1-cells-induced-with-macrophages-conditioned-medium-glucose-and-iron
#6
L Briones, M Andrews, F Pizarro, M Arredondo-Olguín
Obesity is characterized by a chronic inflammatory process, with an increased volume of total adipose tissue, especially visceral, which secretes pro-inflammatory cytokines such as TNF-α and IL-6. Hepcidin (Hpc), a main iron metabolism regulator, is synthetized by an IL-6 stimuli, among others, in liver and adipose tissue, favoring an association between the inflammatory process and iron metabolism. Still there are questions remain regarding the interaction of these factors. Our aim was to study the effect of a macrophage-conditioned medium (MCM) on adipocyte cells challenged with glucose and/or iron...
May 5, 2018: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/29692906/phenotypic-and-molecular-characterization-of-klebsiella-spp-isolates-causing-community-acquired-infections
#7
U Garza-Ramos, H Barrios-Camacho, S Moreno-Domínguez, J Toribio-Jiménez, D Jardón-Pineda, J Cuevas-Peña, A Sánchez-Pérez, J Duran-Bedolla, J Olguín-Rodriguez, A Román-Román
Klebsiella spp. isolates from community-acquired infections were characterized. A total of 39 Klebsiella spp. isolates were obtained from outpatients at four rural hospitals in Mexico (2013-2014). The biochemical tests identified all as being K. pneumoniae . The molecular multiplex-PCR test identified 36 (92.4%) K. pneumoniae isolates and one (2.5%) K. variicola isolate, and phylogenetic analysis of the rpoB gene identified two isolates (5.1%) belonging to K. quasipneumoniae subsp. quasipneumoniae and K...
May 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29684784/pilot-scale-outdoor-production-of-scenedesmus-sp-in-raceways-using-flue-gases-and-centrate-from-anaerobic-digestion-as-the-sole-culture-medium
#8
Ahlem Jebali, F Gabriel Acién, Erika Rodriguez Barradas, Eugenia J Olguín, Sami Sayadi, Emilio Molina Grima
This work investigated the production of Scenedesmus sp. in semi-continuous mode in three pilot-scale outdoor raceways (7.2 m2 ) using flue gas for CO2 supply and centrate from the anaerobic digestion of urban wastewater as the sole nutrient source. Experiments were performed at different culture depths, 5, 10 and 15 cm, while evaluating two centrate concentrations (30% and 45%) at dilution rates of 0.2 and 0.3 d-1 . Under optimal conditions of 30% centrate, 0.3 d-1 dilution rate and a 15 cm culture depth, a maximum biomass productivity of 22...
August 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29661068/correlation-between-radiological-parameters-and-functional-outcomes-in-patients-older-than-60-years-of-age-with-distal-radius-fracture
#9
Rodrigo Gutiérrez-Monclus, Héctor Gutiérrez-Espinoza, Jonathan Zavala-González, Cristian Olguín-Huerta, David Rubio-Oyarzún, Felipe Araya-Quintanilla
BACKGROUND: The relationship between radiographic and functional outcomes is controversial in the elderly. The objective of this study is to determine whether there is a correlation between functional outcome and acceptable distal radius fracture (DRF) alignment in patients older than 60 years of age. METHODS: This correlation study was carried out at the Central Metropolitan Health Service of Chile. A total of 180 patients diagnosed with extra-articular DRF, according to the AO classification, were prospectively recruited...
April 1, 2018: Hand: Official Journal of the American Association for Hand Surgery
https://www.readbyqxmd.com/read/29610276/the-transcriptional-regulator-ccctc-binding-factor-limits-oxidative-stress-in-endothelial-cells
#10
Anna R Roy, Abdalla Ahmed, Peter V DiStefano, Lijun Chi, Nadiya Khyzha, Niels Galjart, Michael D Wilson, Jason E Fish, Paul Delgado-Olguín
The CCCTC-binding factor (CTCF) is a versatile transcriptional regulator required for embryogenesis, but its function in vascular development or in diseases with a vascular component is poorly understood. Here, we found that endothelial Ctcf is essential for mouse vascular development and limits accumulation of reactive oxygen species (ROS). Conditional knockout of Ctcf in endothelial progenitors and their descendants affected embryonic growth, and caused lethality at embryonic day 10.5 because of defective yolk sac and placental vascular development...
June 1, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29584529/characterization-and-pharmacokinetics-of-triamcinolone-acetonide-loaded-liposomes-topical-formulations-for-vitreoretinal-drug-delivery
#11
Juan C Altamirano-Vallejo, Jose Navarro-Partida, Alejandro Gonzalez-De la Rosa, Jane H Hsiao, Jose S Olguín-Gutierrez, Ana C Gonzalez-Villegas, Brian C Keller, Lourdes Bouzo-Lopez, Arturo Santos
PURPOSE: To achieve a safer alternative to intravitreal injection of corticosteroids, we developed and characterized triamcinolone acetonide-loaded liposomes formulations (TA-LFs) to be used topically for vitreoretinal drug delivery. METHODS: Four different 0.2% TA-LFs (TA-LF1 to TA-LF4) were generated and submitted to physicochemical characterization. Posteriorly, an ex vivo diffusion assay was performed using rabbit corneas as membranes. Finally, concentrations of triamcinolone acetonide (TA) were determined by high-performance liquid chromatography in ocular tissues from New Zealand white rabbits after multiple topical doses of TA-LF2 (6 times per day, 14 days)...
March 27, 2018: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29565169/biochemical-and-histological-changes-produced-by-sweeteners-and-cytarabine-in-the-brain-of-young-rats
#12
Ernestina Hernández García, Norma Osnaya Brizuela, Armando Valenzuela Peraza, David Calderón Guzmán, Maribel Ortiz Herrera, Hugo Juárez Olguín, Gerardo Barragán Mejía, Daniel Santamaría Del Ángel, Alberto Rojas Ochoa
OBJECTIVE: The aim of this study was to evaluate the effect of splenda and stevia on dopamine and 5-HIAA levels, and some biomarkers of oxidative stress in the presence of cytarabine. METHODS: Forty-eight young male Wistar rats each with a weight of 80 g (four weeks of age), distributed in six groups of eight animals each, were treated as follows: group 1, control (NaCl 0.9% vehicle); group 2, cytarabine (0.6 g/kg); group 3, stevia (0.6 g/kg); group 4, cytarabine + stevia; group 5, splenda; and group 6, cytarabine + splenda...
February 13, 2018: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/29564766/isolation-and-culture-of-mouse-placental-endothelial-cells
#13
Lijun Chi, Paul Delgado-Olguin
Isolation and culture of endothelial cells (ECs) is a useful tool to study the cellular processes involved in vascular development and vascular maturation. In this chapter, we describe a method to isolate and culture endothelial cells from placentae. This method takes advantage of two transgenes: ROSA26 mT/mG , which drives the expression of GFP upon Cre-mediated recombination, and Tie2-Cre, which expresses Cre driven by the Tie2 promoter in endothelial progenitors and their descendants. GFP-expressing endothelial cells are isolated through fluorescence-activated cell sorting (FACS)...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29564765/isolating-embryonic-cardiac-progenitors-and-cardiac-myocytes-by-fluorescence-activated-cell-sorting
#14
Abdalla Ahmed, Paul Delgado-Olguin
Isolation of highly purified populations of embryonic cardiomyocytes enables the study of congenital cardiac phenotypes at the cellular level. Fluorescent-activated cell sorting (FACS) is normally used to isolate fluorescently tagged cells. Here we describe the isolation of differentiating mouse embryonic cardiac progenitors and cardiomyocytes at embryonic day (E) 9.5 and E13.5, respectively by FACS. Over 50,000 differentiating cardiac progenitors and 200,000 cardiomyocytes can be obtained in a single prep using the methods described...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29564762/live-imaging-of-fetal-intra-abdominal-organs-using-two-photon-laser-scanning-microscopy
#15
Yuhki Koike, Bo Li, Yong Chen, Hiromu Miyake, Carol Lee, Lijun Chi, Richard Wu, Mikihiro Inoue, Keiichi Uchida, Masato Kusunoki, Paul Delgado-Olguin, Agostino Pierro
The processes by which the intra-abdominal organ circulatory system develops in the embryo and during organogenesis are unclear. Previous studies have used fixed tissues to study the development of abdominal organ vasculature in the embryo; however, the intravital circulation of intra-abdominal organs in rodent fetal development has not been studied. This protocol describes a system that uses two-photon laser-scanning microscopy (TPLSM) for real-time observation and quantification of normal and pathologic live fetal intra-abdominal dynamics while the fetus is still connected to the mother via the umbilical cord...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29564758/visualizing-the-vascular-network-in-the-mouse-embryo-and-yolk-sac
#16
Anna R Roy, Paul Delgado-Olguin
Whole mount immunofluorescence is a valuable technique that can be used to visualize vascular networks in early developing embryonic tissues. This technique involves the permeabilization of fixed mouse embryos and yolk sacs, and primary antibody tagging of the endothelial cell marker platelet endothelial cell adhesion molecule 1 (Pecam-1). A secondary antibody tagged with a fluorophore targets the primary antibody, fluorescently labeling endothelial cells and revealing vascular networks.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29564757/mouse-genotyping
#17
Sandra Vuong, Paul Delgado-Olguin
Genotyping is an invaluable tool for identifying organisms carrying genetic variations including insertions and deletions. This method involves the extraction of DNA from animal tissue samples and subsequent amplification of genomic regions of interest by polymerase chain reaction (PCR). The amplified products are analyzed by agarose gel electrophoresis based on their size.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29555646/cell-extrinsic-hematopoietic-impact-of-ezh2-inactivation-in-fetal-liver-endothelial-cells
#18
Wen Hao Neo, Christopher A G Booth, Emanuele Azzoni, Lijun Chi, Paul Delgado-Olguín, Marella F T R de Bruijn, Sten Eirik W Jacobsen, Adam J Mead
Despite the well-established cell-intrinsic role of epigenetic factors in normal and malignant hematopoiesis, their cell-extrinsic role remains largely unexplored. Herein we investigated the hematopoietic impact of inactivating Ezh2 , a key component of polycomb repressive complex 2 (PRC2), in the fetal liver (FL) vascular niche. Hematopoietic specific ( Vav -iCre) Ezh2 inactivation enhanced FL hematopoietic stem cell (HSC) expansion with normal FL erythropoiesis. In contrast, endothelium ( Tie2 -Cre) targeted Ezh2 inactivation resulted in embryonic lethality with severe anemia at embryonic day 13...
May 17, 2018: Blood
https://www.readbyqxmd.com/read/29539615/epidermal-growth-factor-receptor-regulation-of-ewing-sarcoma-cell-function
#19
Nathália Kersting, Bárbara Kunzler Souza, Igor Araujo Vieira, Rafael Pereira Dos Santos, Danielly Brufatto Olguins, Lauro José Gregianin, André Tesainer Brunetto, Algemir Lunardi Brunetto, Rafael Roesler, Caroline Brunetto de Farias, Gilberto Schwartsmann
OBJECTIVE: Ewing sarcoma (ES) is a type of childhood cancer probably arising from stem mesenchymal or neural crest cells. The epidermal growth factor receptor (EGFR) acts as a driver oncogene in many types of solid tumors. However, its involvement in ES remains poorly understood. METHODS: Human SK-ES-1 and RD-ES ES cells were treated with EGF, the EGFR inhibitor tyrphostin (AG1478), or phosphoinositide 3-kinase (PI3K) or extracellular-regulated kinase (ERK)/mitogen-activated kinase (MAPK) inhibitors...
March 14, 2018: Oncology
https://www.readbyqxmd.com/read/29533739/%C3%AE-actin-shows-limited-mobility-and-is-only-required-for-supraphysiological-insulin-stimulated-glucose-transport-in-young-adult-soleus-muscle
#20
Agnete B Madsen, Jonas R Knudsen, Carlos Henriquez-Olguin, Yeliz Angin, Kristien J Zaal, Lykke Sylow, Peter Schjerling, Evelyn Ralston, Thomas E Jensen
Studies in skeletal muscle cell cultures suggest that the cortical actin cytoskeleton is a major requirement for insulin-stimulated glucose transport, implicating the β-actin isoform which, in many cell types, is the main actin isoform. However, it is not clear that β-actin plays such a role in mature skeletal muscle. Neither dependency of glucose transport on β-actin nor actin reorganization upon glucose transport have been tested in mature muscle. To investigate the role of β-actin in fully differentiated muscle, we have performed a detailed characterization of wildtype and muscle-specific β-actin knockout (KO) mice...
March 13, 2018: American Journal of Physiology. Endocrinology and Metabolism
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