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Barbara Bilinska, Anna Hejmej, Malgorzata Kotula-Balak
One approach to visualize internal structures of the testis is histological sectioning of the material. The use of testicular samples allows a detailed analysis of the structure of both seminiferous tubules and the interstitial space. It is worth noting that key role in the control of germ cell development is assigned to Sertoli cells. Thus, in this chapter the special reference is made on visualization of Sertoli cells in the seminiferous epithelium in which they create a specialized microenvironment to support the germ cell development through the formation of the blood-testis barrier (BTB)...
2018: Methods in Molecular Biology
Margarita Lopez-Fernandez, Maria Romero-González, Alix Günther, Pier L Solari, Mohamed L Merroun
This study presents the effect of aqueous uranium speciation (U-hydroxides and U-hydroxo-carbonates) on the interaction of this radionuclide with the cells of the yeast Rhodotorula mucigilanosa BII-R8. This strain was isolated from Spanish bentonites considered as reference materials for the engineered barrier components of the future deep geological repository of radioactive waste. X-ray absorption and infrared spectroscopy showed that the aqueous uranium speciation has no effect on the uranium binding process by this yeast strain...
February 9, 2018: Chemosphere
Marie-Claude Savoy, Pei Mun Woo, Pauline Ulrich, Adrienne Tarres, Pascal Mottier, Aurélien Desmarchelier
A LC-MS/MS method for screening fourteen aminoglycosides in foodstuffs of animal origin is presented. Its scope includes raw materials and processed ingredients but also finished products composed of milk, meat, fish, egg or fat. Aminoglycosides are extracted in an acidic aqueous solution, which is first recovered after centrifugation, then diluted with a basic buffer and finally purified by molecularly imprinted polymer-solid phase extraction (MIP-SPE). Analytes are detected within 8 min by ion-pair reversed phase LC-MS/MS...
February 16, 2018: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Soyun Park, Min-Sung Kim, Woongsik Jang, Jin Kuen Park, Dong Hwan Wang
Herein, solvent-treated bandgap-tunable covalent organic nanosheets (CONs) were prepared via the Stille cross-coupling reaction. These materials are considered useful as interlayers in photovoltaic devices upon the alignment of energy levels between other components. Among various types of solar cells, according to the organic-interlayer study, inverted planar perovskite solar cells (PSCs) are mostly demanded to effectively transport and collect charge carriers due to their high performance. At first, the C-V analysis proved the energy levels of the frontier orbitals for CON-10 and CON-16 nanosheets; this verified the suitability of these nanosheets as hole transport layers (HTLs) with the PEDOT:PSS upon casting both films from DMSO...
February 16, 2018: Nanoscale
Francesco Gallo, Matteo Tebaldi, Carlo Tumscitz
Percutaneous treatment of chronic total occlusions (CTO) is one of the major challenges in contemporary interventional cardiology. These lesions are identified in 15% to 30% of all patients referred for coronary angiography. Percutaneous coronary intervention (PCI) of CTOs is technically challenging and requires familiarity with advanced interventional techniques as well as specialty equipment. The choice of strategy and materials is based on clinical features and coronary anatomy; the correct choice is essential to improve the success probability...
January 2018: Giornale Italiano di Cardiologia
Stefanos Diamantopoulos, Ioannis Kantemiris, Georgios Patatoukas, Maria Dilvoi, Efstathios Efstathopoulos, Vassilis Kouloulias, Kalliopi Platoni
PURPOSE: Plastic phantoms are commonly used in daily routine for dosimetric tasks in radiation therapy. Despite the fact that water is the reference medium according to the dosimetric protocols, measurements with non-water phantoms are easier to be performed. To succeed absorbed dose determination, certain scaling factors have to be applied to the acquired measurements. Taking into account the increased availability of three-dimensional (3D) printing, we attempted to obtain scaling factors for polylactic acid (PLA), a commonly used thermoplastic material for 3D printing...
February 16, 2018: Medical Physics
Jin-Sil Lee, Sang-Yun Kim, Soo-Jin Kwon, Tae-Wan Kim, Soon-Young Jeong, Chul-Ung Kim, Kwan-Young Lee
p-Xylene (PX) is an important large-volume commodity chemical in the petrochemical industry. Therefore, research on producing PX from bio-mass-derived resources is a considerable interest in relation to future alternative technologies. Recently, a new potential route for the direct and selective production of bio-based PX was reported, referred to as the Diels-Alder cycloaddition of biomassderived 2,5-dimethylfuran (DMF) and ethylene followed by the dehydration of an intermediate. Here, we prepared tungstated zirconia (WOx-ZrO2) materials at different calcination temperatures and times as solid acid catalysts for PX production...
February 1, 2018: Journal of Nanoscience and Nanotechnology
Marcello Casa, Maria Sarno, Rosalba Liguori, Claudia Cirillo, Alfredo Rubino, Emanuele Bezzeccheri, Johan Liu, Paolo Ciambelli
Decoration with silver nanoparticles was obtained by coating graphene with a polydopamine layer, able to induce spontaneous metallic nanoparticles formation without any specific chemical interfacial modifier, neither using complex instrumentation. The choice of dopamine was inspired by the composition of adhesive proteins in mussels, related to their robust attach to solid surfaces. The synthesis procedure started from graphite and involved eco-friendly compounds, such as Vitamin C and glucose as reducing agent and water as reaction medium...
February 1, 2018: Journal of Nanoscience and Nanotechnology
L S F Pereira, M F Pedrotti, P Dalla Vecchia, J S F Pereira, E M M Flores
A simple and automated system based on combustion followed by a pyrohydrolysis reaction was proposed for further halogens determination. This system was applied for digestion of soils containing high (90%) and also low (10%) organic matter content for further halogens determination. The following parameters were evaluated: sample mass, use of microcrystalline cellulose and heating time. For analytes absorption, a diluted alkaline solution (6 mL of 25 mmol L -1 NH 4 OH) was used in all experiments. Up to 400 mg of soil with high organic matter content and 100 mg of soil with low organic matter content (mixed with 400 mg of cellulose) could be completely digested using the proposed system...
June 20, 2018: Analytica Chimica Acta
Yuzo Nishizaki, Naoko Sato-Masumoto, Aki Yokota, Tsuyoshi Mikawa, Koichi Nakashima, Taichi Yamazaki, Miho Kuroe, Masahiko Numata, Toshihide Ihara, Yusai Ito, Naoki Sugimoto, Kyoko Sato
To accurately determine carminic acid (CA) and its derivative 4-aminocarminic acid (4-ACA), a novel, high-performance liquid chromatography with photodiode array detector (HPLC/PDA) method using relative molar sensitivity (RMS) was developed. The method requires no analytical standards of CA and 4-ACA; instead it uses the RMS values with respect to caffeine (CAF), which is used as an internal standard. An off-line combination of 1 H-quantitative nuclear magnetic resonance spectroscopy ( 1 H-qNMR) and HPLC/PDA was able to precisely determine the RMSs of CA 274nm /CAF 274nm and 4-ACA 274nm /CAF 274nm ...
February 15, 2018: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Zsolt Varga, Adrian Nicholl, Jozsef Zsigrai, Maria Wallenius, Klaus Mayer
The present work describes a method for the preparation and validation of plutonium age dating reference materials. The test samples prepared in this context could be used to validate experimental protocols for determining the production date of plutonium via the 234 U/ 238 Pu, 235 U/ 239 Pu, 236 U/ 240 Pu and 241 Am/ 241 Pu chronometers. The starting material was prepared using reactor-grade plutonium, which was purified using a dedicated method to guarantee high Pu recovery, while maximizing U and Am separation efficiencies...
February 15, 2018: Analytical Chemistry
Zhidong Luo, Shuran Fan, Chuying Gu, Weicong Liu, Baohong Li, Jianqiang Liu
Metal-organic frameworks (MOFs) are compounds consisting of metal ions or clusters coordinated to organic ligands to form one-, two-, or three-dimensional structures. They are a subclass of coordination polymers (CPs), with the special feature that they are often porous. The organic ligands are sometimes referred to as second building structs. The new porous materials have paid great attention because of the variety of advantages such as tunable components, structural diversity, high loading performance and favorable biodegradability...
February 14, 2018: Current Medicinal Chemistry
M Pettersson, J Öhlen, F Friberg, L C Hydén, C Wallengren, E Sarenmalm, E Carlsson
AIMS AND OBJECTIVES: To describe preoperative communication after a person-centred intervention in nurses' consultations with patients undergoing surgery for colorectal cancer. BACKGROUND: Patients all over the world scheduled for surgery are referred for preoperative consultations with health care professionals. The goal is to assess the risk for perioperative complications, improving quality of care and enabling patients to be prepared for surgery and recovery...
February 15, 2018: Journal of Clinical Nursing
Shubham Chandak, Kedar Tatwawadi, Tsachy Weissman, Inanc Birol
Motivation: New Generation Sequencing (NGS) technologies for genome sequencing produce large amounts of short genomic reads per experiment, which are highly redundant and compressible. However, general-purpose compressors are unable to exploit this redundancy due to the special structure present in the data. Results: We present a new algorithm for compressing reads both with and without preserving the read order. In both cases, it achieves 1.4×-2× compression gain over state-of-the-art read compression tools for datasets containing as many as 3 billion Illumina reads...
February 15, 2018: Bioinformatics
Lindsey A Sharpe, Julia E Vela Ramirez, Olivia M Haddadin, Kathleen A Ross, Balaji Narasimhan, Nicholas A Peppas
Multi-compartmental polymer carriers, referred to as Polyanhydride-Releasing Oral MicroParticle Technology (PROMPT), were formed by a pH-triggered anti-solvent precipitation technique. Polyanhydride nanoparticles were encapsulated into anionic pH-responsive microparticles, allowing for nanoparticle encapsulation in acidic conditions and subsequent release in neutral pH conditions. The effects of varying the nanoparticle composition and feed ratio on the encapsulation efficiency were evaluated. Nanoparticle encapsulation was confirmed by confocal microscopy and infrared spectroscopy...
February 14, 2018: Biomacromolecules
Hongliang Yao, Weiwei Su, Linhuo Lin, Wenjun Pan, Xuan Zeng, Panlin Li
Naringin is a flavanone that widely presents on daily diet and traditional medicinal materials. The ratios of naringin C-2 diastereomers are found different in reported samples, thus suspiciously leading to various functions. In this study, we measured the interconversion of C-2 diastereomers intensively with ultimate high-performance liquid chromatography and circular dichroism spectra. We examined the diastereomeric naringins in fresh citrus fruit, Huajuhong decoction pieces, and naringin tablet; evaluated the impact of tablet production procedures in factory; and monitored the rapid racemization in incubation...
February 14, 2018: Chirality
Qingming Ma, Hao Yuan, Yang Song, Youchuang Chao, Sze Yi Mak, Ho Cheung Shum
Partitioning refers to the distribution of solute molecules in the two immiscible phases of a mixture of two solutions, such as an aqueous two-phase system (ATPS). The partitioning of RNA and peptide has been adjusted in situ to facilitate their assembly into intracellular membraneless organelles. Despite the immense potential of this approach in artificial systems, a partitioning-dependent assembly of macromolecules has been limited, due to the sophisticated processing associated with their in situ modification...
February 14, 2018: Soft Matter
Cheng Ma, Zhengwei Huang, Yuanming Zhu, Xiaona Chen, Vikramjeet Singh, Ying Huang, Xin Pan, Chuanbin Wu
Osmotic pump delivery systems have made significant advances in the past decades for controlled drug release over a long period of time. Usually, osmotic pump products require sophisticated and expensive laser drill technology resulting in increase in production cost and decrease in production efficiency. In this study, a lamotrigine extended release tablet based on a controlled-porosity osmotic pump (CPOP) system was developed to circumvent laser drill technology in reference, Lamictal XR®. The tablet core was coated by a polymer blend of Acryl-EZE® and HPMC E5...
September 1, 2017: Die Pharmazie
Jourdan T Holder, Susan M Reynolds, Linsey W Sunderhaus, René H Gifford
Considerable advancements in cochlear implant technology (e.g., electric acoustic stimulation) and assessment materials have yielded expanded criteria. Despite this, it is unclear whether individuals with better audiometric thresholds and speech understanding are being referred for cochlear implant workup and pursuing cochlear implantation. The purpose of this study was to characterize the mean auditory and demographic profile of adults presenting for preoperative cochlear implant workup. Data were collected prospectively for all adult preoperative workups at Vanderbilt from 2013 to 2015...
January 2018: Trends in Hearing
Barry Reid, Alaric Taylor, Yinong Chen, Benjamin Schmidt-Hansberg, Stefan Guldin
Generating mesoporous films with adequate film thickness and refractive index is a common method to achieve amplitude and phase matching in low-cost interference-based antireflective coatings (ARCs). For high surface energy materials, pores on the 2 - 50 nm, i.e. the sub-wavelength scale, are subject to capillary condensation by surrounding gas phase water molecules which hampers their functioning. In this work, we examine the effect of relative humidity on mesoporous ARCs and present a simple method for the preparation of ARCs with robust operation under variable conditions...
February 14, 2018: ACS Applied Materials & Interfaces
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