Kinga Szigeti, Ivanna Ihnatovych, Emily Notari, Ryu P Dorn, Ivan Maly, Muye He, Barbara Birkaya, Shreyas Prasad, Robin Schwartz Byrne, Dinesh C Indurthi, Erik Nimmer, Yuna Heo, Kolos Retfalvi, Lee Chaves, Norbert Sule, Wilma A Hofmann, Anthony Auerbach, Gregory Wilding, Yongho Bae, Jessica Reynolds
BACKGROUND: Human restricted genes contribute to human specific traits in the immune system. CHRFAM7A, a uniquely human fusion gene, is a negative regulator of the α7 nicotinic acetylcholine receptor (α7 nAChR), the highest Ca2+ conductor of the ACh receptors implicated in innate immunity. Understanding the mechanism of how CHRFAM7A affects the immune system remains unexplored. METHODS: Two model systems are used, human induced pluripotent stem cells (iPSC) and human primary monocytes, to characterize α7 nAChR function, Ca2+ dynamics and decoders to elucidate the pathway from receptor to phenotype...
April 2, 2024: EBioMedicine