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https://www.readbyqxmd.com/read/28728306/-interpretation-of-guidance-for-establishing-the-performance-characteristics-of-in-vitro-diagnostic-devices-for-the-detection-or-detection-and-differentiation-of-high-risk-human-papillomaviruses-issued-by-food-and-drug-administration-of-united-states-and-china
#1
R M Feng, W Chen, Y L Qiao
To establish the standardization of clinical verification designs for in vitro diagnostic devices(IVDs) of human papillomavirus, China Food and Drug Administration (CFDA) and United States Food and Drug Administration (FDA) issued guidelines of detailed study designs and effectiveness evaluation for accuracy validation and clinical performance of nucleic acid detection, respectively, including triage of a typical squamous cells of undetermined significance(ASC-US) patients and validation of combined screening and preliminary screening...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28727918/rapid-sensitive-and-quantitative-detection-of-anti-peg-antibodies-by-mpeg-coated-spr-sensors
#2
Peng Zhang, Fang Sun, Hsiang-Chieh Hung, Priyesh Jain, Kasey Joanne Leger, Shaoyi Jiang
Pre-existing and induced anti-poly(ethylene glycol) (PEG) antibodies (abs) have been shown to be related with limitation of therapeutic efficacy and reduction in tolerance of several therapeutic agents. However, the current methods to detect anti-PEG abs are tedious and usually lack of quantification. A facile, rapid, sensitive and reliable technique to detect anti-PEG abs is highly desired in both research and clinic settings. In this work, we have presented a surface plasmon resonance (SPR) biosensor technique for the detection of anti-PEG abs and compared three PEG surface chemistries...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28727807/digoxin-reveals-a-functional-connection-between-hiv-1-integration-preference-and-t-cell-activation
#3
Alexander Zhyvoloup, Anat Melamed, Ian Anderson, Delphine Planas, Chen-Hsuin Lee, Janos Kriston-Vizi, Robin Ketteler, Andy Merritt, Jean-Pierre Routy, Petronela Ancuta, Charles R M Bangham, Ariberto Fassati
HIV-1 integrates more frequently into transcribed genes, however the biological significance of HIV-1 integration targeting has remained elusive. Using a selective high-throughput chemical screen, we discovered that the cardiac glycoside digoxin inhibits wild-type HIV-1 infection more potently than HIV-1 bearing a single point mutation (N74D) in the capsid protein. We confirmed that digoxin repressed viral gene expression by targeting the cellular Na+/K+ ATPase, but this did not explain its selectivity. Parallel RNAseq and integration mapping in infected cells demonstrated that digoxin inhibited expression of genes involved in T-cell activation and cell metabolism...
July 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28727740/genetically-engineered-suicide-gene-in-mesenchymal-stem-cells-using-a-tet-on-system-for-anaplastic-thyroid-cancer
#4
Senthilkumar Kalimuthu, Ji Min Oh, Prakash Gangadaran, Liya Zhu, Ho Won Lee, Yong Hyun Jeon, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn
Anaplastic thyroid cancer (ATC) is the most aggressive malignancy of the thyroid, during which undifferentiated tumors arise from the thyroid follicular epithelium. ATC has a very poor prognosis due to its aggressive behavior and poor response to conventional therapies. Gene-directed enzyme/prodrug therapy using genetically engineered mesenchymal stromal cells (MSC) is a promising therapeutic strategy. The doxycycline (DOX)-controlled Tet inducible system is the most widely utilized regulatory system and could be a useful tool for therapeutic gene-based therapies...
2017: PloS One
https://www.readbyqxmd.com/read/28727662/paraphilic-thoughts-behaviors-and-sex-addiction-in-a-sample-of-persons-who-use-drugs-a-cross-sectional-study
#5
Jales Clemente, Sandra Cristina Pillon, Jair de Jesus Mari, Claudio Jeronimo da Silva, Paulo Roberto Henrique Santana, Alessandra Diehl
OBJECTIVE: The aim of this study was to evaluate the prevalence of paraphilic thoughts/behaviors (including paraphilic disorders) with and without sexual addiction and sexual-related conditions in a sample of substance disorder patients. METHODS: This was a cross-sectional study of substance-dependent individuals who sought outpatient treatment in São Paulo, Brazil. Data included sociodemographic information, drug of choice, responses to questions about sexual behavior, and to the following standardized questionnaires: (a) Sexual Addiction Screening Test, and (b) Childhood Trauma Questionnaire...
July 19, 2017: Journal of Addiction Medicine
https://www.readbyqxmd.com/read/28727274/the-effect-of-depression-and-side-effects-of-antiepileptic-drugs-on-injuries-in-patients-with-epilepsy
#6
S Gur-Ozmen, M Mula, N Agrawal, H R Cock, D Lozsadi, T J von Oertzen
BACKGROUND AND PURPOSE: People with epilepsy are at increased risk of accidents and injuries but, despite several studies on this subject, data regarding preventable causes are still contradictory. The aim of this study was to investigate the relationship between injuries, side effects of antiepileptic drugs (AEDs) and depression. METHODS: Data from a consecutive sample of adult patients with epilepsy attending the outpatient clinics at St George's University Hospital in London were included...
July 20, 2017: European Journal of Neurology: the Official Journal of the European Federation of Neurological Societies
https://www.readbyqxmd.com/read/28726548/wnt-yap-interactions-during-neural-tissue-patterning-of-human-induced-pluripotent-stem-cells
#7
Julie Bejoy, Liqing Song, Yi Zhou, Yan Li
Human induced pluripotent stem cells (hiPSCs) have special ability to self-assemble into neural spheroids or mini-brain like structures. During the self-assembly process, Wnt signaling plays an important role in regional patterning and establishing positional identity of hiPSC-derived neural progenitors. Recently, the role of Wnt signaling in regulating Yes-associated protein (YAP) expression (nuclear or cytoplasmic), the pivotal regulator during organ growth and tissue generation, has attracted increasing interests...
July 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28724759/cholesterol-25-hydroxylase-inhibits-porcine-reproductive-and-respiratory-syndrome-virus-replication-through-enzyme-activity-dependent-and-independent-mechanisms
#8
Wenting Ke, Liurong Fang, Huiyuan Jing, Ran Tao, Ting Wang, Yang Li, Siwen Long, Dang Wang, Shaobo Xiao
Cholesterol 25-hydroxylase (CH25H) has recently been identified as a host restriction factor that exerts antiviral effects by catalyzing the production of 25-hydroxycholesterol (25HC). CH25H can be rapidly induced upon infection with some viruses. Porcine reproductive and respiratory syndrome virus (PRRSV), an arterivirus, has ranked among the most important swine pathogens since it was discovered in the late 1980s. In this study, we found that PRRSV infection significantly downregulated the expression of CH25H in cells by a so far unknown mechanism, suggesting that CH25H might exert antiviral activity against PRRSV...
July 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28724352/a-permutation-based-non-parametric-analysis-of-crispr-screen-data
#9
Gaoxiang Jia, Xinlei Wang, Guanghua Xiao
BACKGROUND: Clustered regularly-interspaced short palindromic repeats (CRISPR) screens are usually implemented in cultured cells to identify genes with critical functions. Although several methods have been developed or adapted to analyze CRISPR screening data, no single specific algorithm has gained popularity. Thus, rigorous procedures are needed to overcome the shortcomings of existing algorithms. METHODS: We developed a Permutation-Based Non-Parametric Analysis (PBNPA) algorithm, which computes p-values at the gene level by permuting sgRNA labels, and thus it avoids restrictive distributional assumptions...
July 19, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28724038/sexual-addiction-in-drug-addicts-the-impact-of-drug-of-choice-and-poly-addiction
#10
Nelson Antonio, Alessandra Diehl, Marcelo Niel, Sandra Pillon, Lilian Ratto, Maria Carolina Pinheiro, Dartiu Silveira, Thais Zelia Otani, Victor Otani, Quirino Cordeiro, Ricardo Ushida
Objective: To compare the risk of comorbid sexual addiction in a sample of individuals with a diagnosis of substance dependence, stratifying the sample by drug of choice as well as by mono versus polysubstance addiction. Method: All data were collected at Santa Casa de São Paulo, Brazil. The study sample comprised all alcohol or drug dependents admitted to the Addiction Treatment Unit between November 2013 and August 2014. A generalized linear model with a binomial distribution was performed to compare the odds of having a Sexual Addiction Screening Test (SAST) score greater than 6 points in the subgroups analyzed...
May 2017: Revista da Associação Médica Brasileira
https://www.readbyqxmd.com/read/28723871/optimal-anti-retroviral-prophylaxis-in-infants-at-high-risk-of-acquiring-human-immunodeficiency-virus-a-systematic-review
#11
Sara Beste, Shaffiq Essajee, George Siberry, Alisse Hannaford, Jasmeen Dara, Nandita Sugandhi, Martina Penazzato
INTRODUCTION: The risk of perinatal HIV infection can be dramatically reduced through maternal antiretroviral therapy (ART) and infant antiretroviral (ARV) postnatal prophylaxis. The 2013 WHO guidelines recommended four to six weeks of nevirapine or zidovudine as post-natal prophylaxis, with possible extension to 12 weeks for high-risk breastfed infants. A systematic review was undertaken to determine if there is evidence for the WHO to recommend enhanced or extended prophylaxis for high-risk infants...
July 18, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28723278/a-case-of-cannabinoid-hyperemesis-syndrome-with-heliobacter-pylori-and-pre-eclampsia-during-pregnancy
#12
Madeline Manning Meurer, Kalyan Chakrala, Dinesh Gowda, Charles Burns, Randall Kelly, Natalia Schlabritz-Loutsevitch
The condition termed cannabinoid hyperemesis syndrome (CHS) was characterized a decade ago by Allen et al. and includes cyclic episodes of nausea and vomiting and the learned behavior of hot bathing in individuals with chronic cannabis abuse. During pregnancy, the differential diagnosis of this syndrome is challenging since it can be masked by typical symptoms of early pregnancy or by hyperemesis gravidarum, a complication of early pregnancy associated with excessive nausea and vomiting. We herein describe the case of a 21-year-old primigravida patient diagnosed with hyperemesis gravidarum at 6 weeks of gestation and with pre-eclampsia at 35 weeks...
July 19, 2017: Substance Abuse
https://www.readbyqxmd.com/read/28723256/quantitative-testing-of-buprenorphine-and-norbuprenorphine-to-identify-urine-sample-spiking-during-office-based-opioid-treatment
#13
Joji Suzuki, Jennifer Zinser, Mohammed Issa, Claudia Rodriguez
BACKGROUND: Patients may spike urine samples with buprenorphine during office-based opioid treatment to simulate adherence to prescribed buprenorphine, potentially to conceal diversion of medications. However, routine immunoassay screens do not detect instances of spiking, as these would simply result in a positive result. We report on our clinic's experience using quantitative urine testing for buprenorphine and norbuprenorphine to facilitate the identification of urine spiking. METHODS: This is a retrospective chart review of 168 consecutive patients enrolled in outpatient buprenorphine treatment at an urban academic medical setting between May 2013 and August 2014...
July 19, 2017: Substance Abuse
https://www.readbyqxmd.com/read/28723151/fusion-of-structure-and-ligand-based-methods-for-identification-of-novel-cdk2-inhibitors
#14
Priya Mahajan, Gousia Chashoo, Monika Gupta, Amit Kumar, Parvinder Pal Singh, Amit Nargotra
Cyclin dependent kinases plays a central role in cell cycle regulation which makes them a promising target with multifarious therapeutic potential. CDK2 regulates various events of the eukaryotic cell division cycle and the pharmacological evidences indicated that over expression of CDK2 causes abnormal cell-cycle regulation, which was directly associated with hyper proliferation of cancer cells. Therefore, CDK2 is regarded as a potential target molecule for anti-cancer medication. Thus to decline CDK2 activity by potential lead compounds has proved to be an effective treatment for cancer...
July 19, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28722470/novel-potent-inhibitors-of-the-histone-demethylase-kdm1a-lsd1-orally-active-in-a-murine-promyelocitic-leukemia-model
#15
Paolo Trifirò, Anna Cappa, Silvia Brambillasca, Oronza A Botrugno, Maria Rosaria Cera, Roberto Dal Zuffo, Paola Dessanti, Giuseppe Meroni, Florian Thaler, Manuela Villa, Saverio Minucci, Ciro Mercurio, Mario Varasi, Paola Vianello
BACKGROUND: Histone lysine demethylases (KDMs) are well-recognized targets in oncology drug discovery. They function at the post-translation level controlling chromatin conformation and gene transcription. KDM1A is a flavin adenine dinucleotide-dependent amine oxidase, overexpressed in several tumor types, including acute myeloid leukemia, neuroblastoma and non-small-cell lung cancer. Among the many known monoamine oxidase inhibitors screened for KDM1A inhibition, tranylcypromine emerged as a moderately active hit, which irreversibly binds to the flavin adenine dinucleotide cofactor...
July 19, 2017: Future Medicinal Chemistry
https://www.readbyqxmd.com/read/28722340/a-novel-small-molecule-inhibitor-of-prion-replication-and-mutant-prion-protein-toxicity
#16
Tania Massignan, Valeria Sangiovanni, Silvia Biggi, Claudia Stincardini, Saioa R Elezgarai, Giulia Maietta, Ivan A Andreev, Nina K Ratmanova, Dmitry S Belov, Evgeny R Lukyanenko, Grigory M Belov, Maria Letizia Barreca, Andrea Altieri, Alexander V Kurkin, Emiliano Biasini
Prion diseases are a class of neurodegenerative disorders characterized by the accumulation in the brain of a self-replicating, misfolded isoform (PrPSc) of the cellular prion protein (PrPC), a cell-surface glycoprotein of uncertain function. Emerging evidence suggests that PrPC may passively serve as a substrate for the replication of PrPSc, and actively transduce its toxic effects to neuronal cells. The vast majority of previous drug screening campaigns have only targeted PrPSc replication, with largely unsuccessful results...
July 19, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28721669/mimicking-parkinson-s-disease-in-a-dish-merits-and-pitfalls-of-the-most-commonly-used-dopaminergic-in-vitro-models
#17
REVIEW
Fernanda Martins Lopes, Ivi Juliana Bristot, Leonardo Lisbôa da Motta, Richard B Parsons, Fabio Klamt
Parkinson's disease (PD) is the second most common neurodegenerative disorder and has both unknown etiology and non-curative therapeutic options. Patients begin to present the classic motor symptoms of PD-tremor at rest, bradykinesia and rigidity-once 50-70% of the dopaminergic neurons of the nigrostriatal pathway have degenerated. As a consequence of this, it is difficult to investigate the early-stage events of disease pathogenesis. In vitro experimental models are used extensively in PD research because they present a controlled environment that enables the direct investigation of the early molecular mechanisms that are potentially involved with dopaminergic degeneration, as well as for the screening of potential therapeutic drugs...
July 18, 2017: Neuromolecular Medicine
https://www.readbyqxmd.com/read/28721630/in-situ-salification-in-polar-solvents-a-paradigm-for-enabling-drug-delivery-of-weakly-ionic-drugs-as-amorphous-solid-dispersion
#18
Rashmi Nair, Ioorika Lamare, Nirbhay Kumar Tiwari, Punna Rao Ravi, Raviraj Pillai
Solubility challenge for a poorly water-soluble drug gets further intensified when it is weakly ionic because the most common solubility enhancement technique, salt formation, becomes less feasible. Salt screening for such drugs often concludes with either a difficult to crystalize salt or an unstable salt, leading the scientists to explore other solubility enhancement technique like amorphous solid dispersions which is comparatively costlier, time-consuming and may require use of hazardous organic solvents...
July 18, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28721301/synthesis-and-characterization-of-n-mannich-based-prodrugs-of-ciprofloxacin-and-norfloxacin-in-vitro-anthelmintic-and-cytotoxic-evaluation
#19
Mona Piplani, Harish Rajak, Prabodh Chander Sharma
Prodrugs, the inert derivatives of existing drugs have successfully contributed to the modification of their physicochemical properties. The improved antimicrobial potential due to enhanced lipophilicity of some of the synthesized prodrugs of antibacterial agents by various schemes has already been reported. In the current study, synthesis, characterization, and biological evaluation of some more lipid based prodrugs/compounds of ciprofloxacin and norfloxacin has been carried out. The synthesized prodrugs/compounds have been screened for anthelmintic activity using Indian earthworms and cytotoxic activity against human lung cancer cell lines A-549 employing sulforhodamine B (SRB) assay method...
July 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/28721038/toxicity-based-toxicokinetic-toxicodynamic-assessment-of-bioaccumulation-and-nanotoxicity-of-zerovalent-iron-nanoparticles-in-caenorhabditis-elegans
#20
Ying-Fei Yang, Yi-Jun Lin, Chung-Min Liao
Elucidating the relationships between the toxicity-based-toxicokinetic (TBTK)/toxicodynamic (TD) properties of engineered nanomaterials and their nanotoxicity is crucial for human health-risk analysis. Zerovalent iron (Fe(0)) nanoparticles (NPs) are one of the most prominent NPs applied in remediating contaminated soils and groundwater. However, there are concerns that Fe(0)NP application contributes to long-term environmental and human health impacts. The nematode Caenorhabditis elegans is a surrogate in vivo model that has been successfully applied to assess the potential nanotoxicity of these nanomaterials...
2017: International Journal of Nanomedicine
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