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https://www.readbyqxmd.com/read/28225009/transcriptomic-responses-of-the-olive-fruit-fly-bactrocera-oleae-and-its-symbiont-candidatus-erwinia-dacicola-to-olive-feeding
#1
Nena Pavlidi, Anastasia Gioti, Nicky Wybouw, Wannes Dermauw, Michael Ben-Yosef, Boaz Yuval, Edouard Jurkevich, Anastasia Kampouraki, Thomas Van Leeuwen, John Vontas
The olive fruit fly, Bactrocera oleae, is the most destructive pest of olive orchards worldwide. The monophagous larva has the unique capability of feeding on olive mesocarp, coping with high levels of phenolic compounds and utilizing non-hydrolyzed proteins present, particularly in the unripe, green olives. On the molecular level, the interaction between B. oleae and olives has not been investigated as yet. Nevertheless, it has been associated with the gut obligate symbiotic bacterium Candidatus Erwinia dacicola...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28222361/biological-and-catalytic-applications-of-green-synthesized-fluorescent-n-doped-carbon-dots-using-hylocereus-undatus
#2
Velusamy Arul, Thomas Nesakumar Jebakumar Immanuel Edison, Yong Rok Lee, Mathur Gopalakrishnan Sethuraman
In this work, a simple hydrothermal route for the synthesis of fluorescent nitrogen doped carbon dots (N-CDs) is reported. The Hylocereus undatus (H. undatus) extract and aqueous ammonia are used as carbon and nitrogen source, respectively. The optical properties of synthesized N-CDs are analyzed using UV-Visible (UV-Vis) and fluorescence spectroscopy. The surface morphology, elemental composition, crystallinity and functional groups present in the N-CDs are examined using high resolution transmission electron microscopy (HR-TEM) with energy dispersive spectroscopy (EDS), selected area electron diffraction (SAED), X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy, respectively...
February 13, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28217828/photophysics-of-egfp-e222h-mutant-with-comparisons-to-model-chromophores-excited-state-pk-s-progressions-quenching-and-exciton-interaction
#3
William Kirk, Thomas Allen, Elena Atanasova, William Wessels, Janet Yao, Franklyn Prendergast
A novel version of the well-known and commercially successful Green Fluorescent Protein (GFP) variant known as EGFP, with an introduced E222H mutation, was produced in this laboratory. Given the current state of hypotheses about the role of glutamate 222, and the observed dominance of the phenolate absorption with an E222H variant observed from earlier study, the new mutant was considered a natural choice to investigate more fully the acid-base behavior of the chromophore in absorption and fluorescence. The bulk of this investigation concerns fitting the excitation, emission and absorption spectra to vibrational progressions of a novel 'q-deformed' type at various values of pH, and protein concentration...
February 20, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28217242/investigating-state-restriction-in-fluorescent-protein-fret-using-time-resolved-fluorescence-and-anisotropy
#4
Thomas S Blacker, WeiYue Chen, Edward Avezov, Richard J Marsh, Michael R Duchen, Clemens F Kaminski, Angus J Bain
Most fluorescent proteins exhibit multiexponential fluorescence decays, indicating a heterogeneous excited state population. FRET between fluorescent proteins should therefore involve multiple energy transfer pathways. We recently demonstrated the FRET pathways between EGFP and mCherry (mC), upon the dimerization of 3-phosphoinositide dependent protein kinase 1 (PDK1), to be highly restricted. A mechanism for FRET restriction based on a highly unfavorable κ(2) orientation factor arising from differences in donor-acceptor transition dipole moment angles in a far from coplanar and near static interaction geometry was proposed...
January 26, 2017: Journal of Physical Chemistry. C, Nanomaterials and Interfaces
https://www.readbyqxmd.com/read/28214496/new-diagnostic-modalities-in-the-evaluation-of-lymphedema
#5
REVIEW
Thomas F O'Donnell, John C Rasmussen, Eva M Sevick-Muraca
OBJECTIVE: Currently, lymphedema (LED) is typically diagnosed clinically on the basis of a patient's history and characteristic physical findings. Whereas the diagnosis of LED is sometimes confirmed by lymphoscintigraphy (LSG), the technique is limited in both its ability to identify disease and to guide therapy. Recent advancements provide opportunities for new imaging techniques not only to assist in the diagnosis of LED, based on anatomic changes, but also to assess contractile function and to guide therapeutic intervention...
March 2017: Journal of Vascular Surgery. Venous and Lymphatic Disorders
https://www.readbyqxmd.com/read/28212707/astrobiological-implications-of-dim-light-phototrophy-in-deep-sea-red-clays
#6
Anindita Das, Tanya Singh, P A LokaBharathi, Prashant K Dhakephalkar, Sweta Mallik, Pranav R Kshirsagar, N H Khadge, B Nagender Nath, Satadru Bhattacharya, Aditya Kumar Dagar, Prabhjot Kaur, Dwijesh Ray, Anil D Shukla, Christabelle E G Fernandes, Sheryl O Fernandes, Tresa Remya A Thomas, Mamatha S S, Babu Shashikant Mourya, Ram Murti Meena
Red clays of Central Indian Basin (CIB) under influence of trace of Rodriguez Triple Junction exhibited chemoautotrophy, low temperature hydrothermal alterations and photoautotrophic potential. Seamount flank TVBC-08, hosting such signatures revealed dominance of aerobic anoxygenic phototroph Erythrobacter, with 93% of total 454 pyrosequencing tags. Subsequently, enrichments for both aerobic (Erythrobacter) and anaerobic anoxygenic phototrophs (green and purple sulphur bacteria) under red and white LED light illumination, with average irradiance 30...
February 2017: Life Sciences in Space Research
https://www.readbyqxmd.com/read/28197579/protein-diffusion-in-a-bicontinuous-microemulsion-inducing-sub-diffusion-by-tuning-the-water-domain-size
#7
Ralph Neubauer, Sebastian Höhn, Martin Dulle, Alain Lapp, Christoph Schulreich, Thomas Hellweg
We study the diffusion of an enhanced green fluorescent protein (GFP+) in bicontinuous sugar-surfactant based microemulsions. The size of the water domains in such systems is controlled by changes of the oil-to-water ratio. Hence, microemulsions allow to produce confinement effects in a controlled way. At high water content the protein is found to exhibit Fickian diffusion. Decreasing the water domain size leads to a slowing down of the protein diffusion and sub-diffusive behavior is obtained on the scale observed by fluorescence correlation spectroscopy...
February 15, 2017: Soft Matter
https://www.readbyqxmd.com/read/28190649/factors-associated-with-deciding-between-risk-reducing-salpingo-oophorectomy-and-ovarian-cancer-screening-among-high-risk-women-enrolled-in-gog-0199-an-nrg-oncology-gynecologic-oncology-group-study
#8
Phuong L Mai, Marion Piedmonte, Paul K Han, Richard P Moser, Joan L Walker, Gustavo Rodriguez, John Boggess, Thomas J Rutherford, Oliver Zivanovic, David E Cohn, J Tate Thigpen, Robert M Wenham, Michael L Friedlander, Chad A Hamilton, Jamie Bakkum-Gamez, Alexander B Olawaiye, Martee L Hensley, Mark H Greene, Helen Q Huang, Lari Wenzel
OBJECTIVES: Women at increased genetic risk of ovarian cancer (OC) are recommended to have risk-reducing salpingo-oophorectomy (RRSO) after completion of reproductive planning. Effective screening has not been established, and novel screening modalities are being evaluated. METHODS: Participants chose either RRSO or a novel OC screening regimen (OCS) as their risk management option, and provided demographic and other data on BRCA mutation status, cancer worry, perceived intervention risks/benefits, perceived cancer risk, and quality-of-life at enrollment...
February 10, 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/28190401/rapid-transcriptional-plasticity-of-duplicated-gene-clusters-enables-a-clonally-reproducing-aphid-to-colonise-diverse-plant-species
#9
Thomas C Mathers, Yazhou Chen, Gemy Kaithakottil, Fabrice Legeai, Sam T Mugford, Patrice Baa-Puyoulet, Anthony Bretaudeau, Bernardo Clavijo, Stefano Colella, Olivier Collin, Tamas Dalmay, Thomas Derrien, Honglin Feng, Toni Gabaldón, Anna Jordan, Irene Julca, Graeme J Kettles, Krissana Kowitwanich, Dominique Lavenier, Paolo Lenzi, Sara Lopez-Gomollon, Damian Loska, Daniel Mapleson, Florian Maumus, Simon Moxon, Daniel R G Price, Akiko Sugio, Manuella van Munster, Marilyne Uzest, Darren Waite, Georg Jander, Denis Tagu, Alex C C Wilson, Cock van Oosterhout, David Swarbreck, Saskia A Hogenhout
BACKGROUND: The prevailing paradigm of host-parasite evolution is that arms races lead to increasing specialisation via genetic adaptation. Insect herbivores are no exception and the majority have evolved to colonise a small number of closely related host species. Remarkably, the green peach aphid, Myzus persicae, colonises plant species across 40 families and single M. persicae clonal lineages can colonise distantly related plants. This remarkable ability makes M. persicae a highly destructive pest of many important crop species...
February 13, 2017: Genome Biology
https://www.readbyqxmd.com/read/28185644/bicuspid-aortic-valve-morphotype-correlates-with-regional-antioxidant-gene%C3%A2-expression-profiles-in-the-proximal%C3%A2-ascending-aorta
#10
Julie A Phillippi, Jennifer C Hill, Marie Billaud, Benjamin R Green, Mary P Kotlarczyk, Thomas G Gleason
BACKGROUND: Bicuspid aortic valve (BAV) is associated with asymmetric dilatation of the proximal ascending aorta. We previously demonstrated increased susceptibility of smooth muscle cells to oxidative stress in the BAV-aneurysmal aorta and hypothesized that antioxidant expression is regionally defined and influenced by the BAV morphotype. METHODS: BAV valve morphology was defined according to number of raphes: type 0 (0 raphes), type 1 (1 raphe), or type 2 (2 raphes) and by the raphe location among the left (L), right (R) or non (N) coronary cusps...
February 6, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28183912/merging-paleobiology-with-conservation-biology-to-guide-the-future-of-terrestrial-ecosystems
#11
REVIEW
Anthony D Barnosky, Elizabeth A Hadly, Patrick Gonzalez, Jason Head, P David Polly, A Michelle Lawing, Jussi T Eronen, David D Ackerly, Ken Alex, Eric Biber, Jessica Blois, Justin Brashares, Gerardo Ceballos, Edward Davis, Gregory P Dietl, Rodolfo Dirzo, Holly Doremus, Mikael Fortelius, Harry W Greene, Jessica Hellmann, Thomas Hickler, Stephen T Jackson, Melissa Kemp, Paul L Koch, Claire Kremen, Emily L Lindsey, Cindy Looy, Charles R Marshall, Chase Mendenhall, Andreas Mulch, Alexis M Mychajliw, Carsten Nowak, Uma Ramakrishnan, Jan Schnitzler, Kashish Das Shrestha, Katherine Solari, Lynn Stegner, M Allison Stegner, Nils Chr Stenseth, Marvalee H Wake, Zhibin Zhang
Conservation of species and ecosystems is increasingly difficult because anthropogenic impacts are pervasive and accelerating. Under this rapid global change, maximizing conservation success requires a paradigm shift from maintaining ecosystems in idealized past states toward facilitating their adaptive and functional capacities, even as species ebb and flow individually. Developing effective strategies under this new paradigm will require deeper understanding of the long-term dynamics that govern ecosystem persistence and reconciliation of conflicts among approaches to conserving historical versus novel ecosystems...
February 10, 2017: Science
https://www.readbyqxmd.com/read/28177692/neuromuscular-manifestations-of-work-related-myalgia-in-women-specific-to-extensor-carpi-radialis-brevis
#12
Howard J Green, Don Ranney, Natasha Kyle, David Lounsbury, Ian C Smith, Riley Stewart, Melissa M Thomas, Heather Tick, A Russell Tupling
This study assessed neuromuscular function in the extensor carpi radialis brevis (ECRB) of female workers diagnosed with work-related myalgia (WRM, n=14, age 45.2±1.9 years) and the ECRB of healthy controls (CON, n=10, age 34.6±2.5 years). Groups were compared on voluntary and electrically evoked functional responses at rest (Pre), immediately following a 5 min repetitive task (Post-0) performed at 60% maximal voluntary contraction (MVC), and after 5 min of recovery (Post-5). Despite near complete motor unit activation (MUA)(CON 98±1% vs WRM 99±1%), at Pre, WRM produced 26% less (P<0...
November 24, 2016: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28161102/residual-radioactivity-of-treated-green-diamonds
#13
Philippe Cassette, Franck Notari, Marie-Christine Lépy, Candice Caplan, Sylvie Pierre, Thomas Hainschwang, Emmanuel Fritsch
Treated green diamonds can show residual radioactivity, generally due to immersion in radium salts. We report various activity measurements on two radioactive diamonds. The activity was characterized by alpha and gamma ray spectrometry, and the radon emanation was measured by alpha counting of a frozen source. Even when no residual radium contamination can be identified, measurable alpha and high-energy beta emissions could be detected. The potential health impact of radioactive diamonds and their status with regard to the regulatory policy for radioactive products are discussed...
January 29, 2017: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/28156133/phycoremediation-of-municipal-wastewater-by-microalgae-to-produce-biofuel
#14
Amit Kumar Singh, Nikunj Sharma, Humaira Farooqi, Malik Zainul Abdin, Thomas Mock, Shashi Kumar
Municipal wastewater (WW), if not properly remediated, poses a threat to the environment and human health by carrying significant loads of nutrients and pathogens. These contaminants pollute rivers, lakes and natural reservoirs where they cause eutrophication and pathogen-mediated diseases. However, the high nutrient content of WW makes it an ideal environment for remediation with microalgae that require high nutrient concentrations for growth and are not susceptible to toxins and pathogens. Given that an appropriate algal strain is used for remediation, the incurred biomass can be refined for the production of biofuel...
February 3, 2017: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/28154576/regulatory-shifts-in-plastid-transcription-play-a-key-role-in-morphological-conversions-of-plastids-during-plant-development
#15
REVIEW
Monique Liebers, Björn Grübler, Fabien Chevalier, Silva Lerbs-Mache, Livia Merendino, Robert Blanvillain, Thomas Pfannschmidt
Plastids display a high morphological and functional diversity. Starting from an undifferentiated small proplastid, these plant cell organelles can develop into four major forms: etioplasts in the dark, chloroplasts in green tissues, chromoplasts in colored flowers and fruits and amyloplasts in roots. The various forms are interconvertible into each other depending on tissue context and respective environmental condition. Research of the last two decades uncovered that each plastid type contains its own specific proteome that can be highly different from that of the other types...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28150483/from-gold-standard-resection-to-reproducible-future-standard-endoscopic-enucleation-of-the-prostate-eep-what-we-know-about-anatomical-enucleation
#16
Richard Naspro, Fernando Gomez Sancha, Michele Manica, Agostino Meneghini, Sascha Ahyai, Tevita Aho, Cristian Fiori, Ivano Vavassori, Luigi F DA Pozzo, Vito Pansadoro, Francesco Montorsi, Thomas R Herrmann
INTRODUCTION: Open prostatectomy (OP) and transurethral resection of the prostate (TURP) have traditionally been the most common surgical approaches for the treatment of benign prostatic hyperplasia causing bladder outlet obstruction and have certainly passed the test of time. In time, many endoscopic surgical procedures have been described as an alternative mini-invasive treatment. Holmium laser enucleation (HoLEP) guaranteed functional outcomes similar to OP and TURP with lower peri-operative complication rates for any prostate size...
February 1, 2017: Minerva Urologica e Nefrologica, the Italian Journal of Urology and Nephrology
https://www.readbyqxmd.com/read/28143870/early-detection-of-ovarian-cancer-using-the-risk-of-ovarian-cancer-algorithm-with-frequent-ca125-testing-in-women-at-increased-familial-risk-combined-results-from-two-screening-trials
#17
Steven J Skates, Mark H Greene, Saundra S Buys, Phuong L Mai, Powel H Brown, Marion Piedmonte, Gustavo C Rodriguez, John O Schorge, Mark Sherman, Mary B Daly, Thomas J Rutherford, Wendy R Brewster, David M O'Malley, Edward E Partridge, John Boggess, Charles W Drescher, Claudine Isaacs, Andrew Berchuck, Susan M Domchek, Susan A Davidson, Robert P Edwards, Steven A Elg, Katie Wakeley, Kelly-Anne Phillips, Deborah K Armstrong, Ira R Horowitz, Carol J Fabian, Joan Walker, Patrick M Sluss, William R Welch, Lori Minasian, Nora Horick, Carol H Kasten, Susan Nayfield, David S Alberts, Dianne M Finkelstein, Karen Lu
PURPOSE: Women at familial/genetic ovarian cancer risk often undergo screening despite unproven efficacy. Research suggests each woman has her own CA125 baseline; significant increases above this level may identify cancers earlier than standard 6-12 monthly CA125>35U/mL. EXPERIMENTAL DESIGN: Data from prospective Cancer Genetics Network and Gynecologic Oncology Group trials, which screened 3,692 women (13,080 woman-screening years) with a strong breast/ovarian cancer family history or BRCA1/2 mutations, were combined to assess a novel screening strategy...
January 31, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28141933/the-social-value-of-childhood-vaccination-in-the-united-states
#18
Thomas J Philipson, Julia Thornton Snider, Ayman Chit, Sarah Green, Philip Hosbach, Taylor Tinkham Schwartz, Yanyu Wu, Wade M Aubry
OBJECTIVES: To determine the lifetime social value of using the guideline-recommended vaccines for children born in the United States in 2009. STUDY DESIGN: This study utilized an economic model with parameter values sourced from clinical and observational data, as well as the literature. METHODS: The model quantified the health effects of routine vaccination for 14 diseases in terms of quality-adjusted life-years (QALYs) saved. The health effects were then valued by applying an economic value of a QALY...
January 2017: American Journal of Managed Care
https://www.readbyqxmd.com/read/28139919/genome-resolved-meta-omics-ties-microbial-dynamics-to-process-performance-in-biotechnology-for-thiocyanate-degradation
#19
Rose S Kantor, Robert J Huddy, Ramsunder Iyer, Brian C Thomas, Christopher T Brown, Karthik Anantharaman, Susannah Tringe, Robert L Hettich, Susan T L Harrison, Jillian F Banfield
Remediation of industrial wastewater is important for preventing environmental contamination and enabling water reuse. Biological treatment for one industrial contaminant, thiocyanate (SCN(-)), relies upon microbial hydrolysis, but this process is sensitive to high loadings. To examine the activity and stability of a microbial community over increasing SCN(-) loadings, we established and operated a continuous-flow bioreactor fed increasing loadings of SCN(-). A second reactor was fed ammonium sulfate to mimic breakdown products of SCN(-)...
February 13, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28137881/alterations-of-p11-in-brain-tissue-and-peripheral-blood-leukocytes-in-parkinson-s-disease
#20
Holly Green, Xiaoqun Zhang, Katarina Tiklova, Nikolaos Volakakis, Lovisa Brodin, Louise Berg, Paul Greengard, Thomas Perlmann, Per Svenningsson
Individuals with Parkinson's disease (PD) often suffer from comorbid depression. P11 (S100A10), a member of the S100 family of proteins, is expressed widely throughout the body and is involved in major depressive disorder and antidepressant response. Central p11 levels are reduced in postmortem tissue from depressed individuals; however, p11 has not yet been investigated in PD patients with depression or those without depression. We investigated p11 levels in postmortem PD brains and assessed whether peripheral p11 levels correlate with disease severity...
January 30, 2017: Proceedings of the National Academy of Sciences of the United States of America
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