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bell's palcy

A W Bell, M A Ward, W P Blackstock, H N Freeman, J S Choudhary, A P Lewis, D Chotai, A Fazel, J N Gushue, J Paiement, S Palcy, E Chevet, M Lafrenière-Roula, R Solari, D Y Thomas, A Rowley, J J Bergeron
A mass spectrometric analysis of proteins partitioning into Triton X-114 from purified hepatic Golgi apparatus (84% purity by morphometry, 122-fold enrichment over the homogenate for the Golgi marker galactosyl transferase) led to the unambiguous identification of 81 proteins including a novel Golgi-associated protein of 34 kDa (GPP34). The membrane protein complement was resolved by SDS-polyacrylamide gel electrophoresis and subjected to a hierarchical approach using delayed extraction matrix-assisted laser desorption ionization mass spectrometry characterization by peptide mass fingerprinting, tandem mass spectrometry to generate sequence tags, and Edman sequencing of proteins...
February 16, 2001: Journal of Biological Chemistry
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