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https://www.readbyqxmd.com/read/28210260/from-the-cradle-to-the-grave-the-role-of-macrophages-in-erythropoiesis-and-erythrophagocytosis
#1
REVIEW
Thomas R L Klei, Sanne M Meinderts, Timo K van den Berg, Robin van Bruggen
Erythropoiesis is a highly regulated process where sequential events ensure the proper differentiation of hematopoietic stem cells into, ultimately, red blood cells (RBCs). Macrophages in the bone marrow play an important role in hematopoiesis by providing signals that induce differentiation and proliferation of the earliest committed erythroid progenitors. Subsequent differentiation toward the erythroblast stage is accompanied by the formation of so-called erythroblastic islands where a central macrophage provides further cues to induce erythroblast differentiation, expansion, and hemoglobinization...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28202115/-clinical-features-and-pathogenic-gene-detection-of-diamond-blackfan-anemia
#2
Xu He, Zhi-Liang Xu
OBJECTIVE: To investigate the clinical features of Diamond-Blackfan anemia (DBA) and related pathogenic genes. METHODS: A retrospective analysis was performed for the clinical data of two children with DBA, and related literature was reviewed. RESULTS: The two children with DBA (2-3 months old) manifested with severe normochromic normocytic anemia, decreased reticulocyte count, and increased serum iron and serum ferritin. Normal white blood cell and platelet counts were noted in the two patients...
February 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/28197649/dose-response-relationship-of-temozolomide-determined-by-the-pig-a-comet-and-micronucleus-assay
#3
M Guérard, G Johnson, S Dertinger, G Duran-Pacheco, J Funk, A Zeller
Temozolomide (TMZ), a monofunctional alkylating agent, was selected as a model compound to determine its quantitative genotoxic dose-response relationship in different tissues (blood, liver, and jejunum) and endpoints [Pig-a-, comet-, and micronucleus assay (MNT)] in male rats. TMZ was administered p.o. over 5 consecutive days (day 1-5), followed by a treatment-free period of 50 days (day 6-56) and a final administration prior to necropsy (day 57-59). TMZ showed a dose-dependent increase in DNA damage in all interrogated endpoints...
February 15, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28195038/structure-of-the-malaria-vaccine-candidate-antigen-cyrpa-and-its-complex-with-a-parasite-invasion-inhibitory-antibody
#4
Paola Favuzza, Elena Guffart, Marco Tamborrini, Bianca Scherer, Anita M Dreyer, Arne C Rufer, Johannes Erny, Joerg Hoernschemeyer, Ralf Thoma, Georg Schmid, Bernard Gsell, Araceli Lamelas, Joerg Benz, Catherine Joseph, Hugues Matile, Gerd Pluschke, Markus G Rudolph
Invasion of erythrocytes by Plasmodial merozoites is a composite process involving the interplay of several proteins. Among them, the Plasmodium falciparum Cysteine-Rich Protective Antigen (PfCyRPA) is a crucial component of a ternary complex, including Reticulocyte binding-like Homologous protein 5 (PfRH5) and the RH5-interacting protein (PfRipr), essential for erythrocyte invasion. Here we present the crystal structure of PfCyRPA and of its complex with the antigen-binding fragment of a parasite growth inhibitory antibody...
February 14, 2017: ELife
https://www.readbyqxmd.com/read/28185829/a-systematic-review-of-the-literature-for-severity-predictors-in-children-with-sickle-cell-anemia
#5
Emily Riehm Meier, Ross M Fasano, Paul R Levett
All patients with HbSS (SCA) share the same genetic mutation but the clinical phenotype is variable and difficult to predict early in life. A reliable severity predictor would be invaluable toward directing therapeutic decisions in those patients at highest risk of SCA complications. A search of PubMed, Cochrane Clinical Trials Register, and Scopus was performed to determine which SCA severity predictors have been validated in pediatric patients. The full text of 94 of the 590 references identified was reviewed based on the title/abstract...
February 2, 2017: Blood Cells, Molecules & Diseases
https://www.readbyqxmd.com/read/28177364/effects-of-group-size-floor-space-allowance-during-the-nursery-phase-of-production-on-growth-physiology-and-hematology-in-replacement-gilts
#6
S R Callahan, A J Cross, A E DeDecker, M D Lindemann, M J Estienne
The objective was to determine effects of nursery group-size-floor space allowance on growth, physiology, and hematology of replacement gilts. A 3 × 3 factorial arrangement of treatments was used wherein gilts classified as large, medium, or small ( = 2537; BW = 5.6 ± 0.6 kg) from 13 groups of weaned pigs were placed in pens of 14, 11, or 8 pigs resulting in floor space allowances of 0.15, 0.19, or 0.27 m/pig, respectively. Pigs were weighed on d 0 (weaning) and d 46 (exit from nursery). The ADG was affected by group-size-floor space allowance × pig size ( = 0...
January 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28166632/metabolomics-based-elucidation-of-active-metabolic-pathways-in-erythrocytes-and-hsc-derived-reticulocytes
#7
Anubhav Srivastava, Krystal J Evans, Anna Elizabeth Sexton, Louis Schofield, Darren John Creek
A detailed analysis of the metabolic state of human stem cell derived erythrocytes allowed us to characterize the existence of active metabolic pathways in younger reticulocytes and compare them to mature erythrocytes. Using high resolution LC-MS based, untargeted metabolomics, we found that reticulocytes had a comparatively much richer repertoire of metabolites, which spanned a range of metabolite classes. An untargeted metabolomics analysis using stable isotope-labelled glucose showed that only glycolysis and the pentose phosphate pathway actively contributed to the biosynthesis of metabolites in erythrocytes, and these pathways were upregulated in reticulocytes...
February 7, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28153778/accelerating-the-clinical-development-of-protein-based-vaccines-for-malaria-by-efficient-purification-using-a-four-amino-acid-c-terminal-c-tag
#8
Jing Jin, Kathryn A Hjerrild, Sarah E Silk, Rebecca E Brown, Geneviève M Labbé, Jennifer M Marshall, Katherine E Wright, Sandra Bezemer, Stine B Clemmensen, Sumi Biswas, Yuanyuan Li, Aadil El-Turabi, Alexander D Douglas, Pim Hermans, Frank J Detmers, Willem A de Jongh, Matthew K Higgins, Rebecca Ashfield, Simon J Draper
Development of bespoke biomanufacturing processes remains a critical bottleneck for translational studies, in particular when modest quantities of a novel product are required for proof-of-concept Phase I/II clinical trials. In these instances the ability to develop a biomanufacturing process quickly and relatively cheaply, without risk to product quality or safety, provides a great advantage by allowing new antigens or concepts in immunogen design to more rapidly enter human testing. These challenges with production and purification are particularly apparent when developing recombinant protein-based vaccines for difficult parasitic diseases, with Plasmodium falciparum malaria being a prime example...
January 30, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28151426/decreasing-tfr1-expression-reverses-anemia-and-hepcidin-suppression-in-%C3%AE-thalassemic-mice
#9
Huihui Li, Tenzin Choesang, Weili Bao, Huiyong Chen, Maria Feola, Daniel Garcia-Santos, Jie Li, Shuming Sun, Antonia Follenzi, Petra Pham, Jing Liu, Jinghua Zhang, Prem Ponka, Xiuli An, Narla Mohandas, Robert Fleming, Stefano Rivella, Guiyuan Li, Yelena Ginzburg
Iron availability for erythropoiesis and its dysregulation in β-thalassemia are incompletely understood. We previously demonstrated that exogenous apo-transferrin leads to more effective erythropoiesis, decreasing erythroferrone and de-repressing hepcidin in β-thalassemic mice. Transferrin-bound iron binding to transferrin receptor 1 (TfR1) is essential for cellular iron delivery during erythropoiesis. We hypothesize that apo-transferrin's effect is mediated via decreased TfR1 expression, and evaluate TfR1 expression in β-thalassemic mice in vivo and in vitro with and without added apo-transferrin...
1, 2017: Blood
https://www.readbyqxmd.com/read/28149014/using-red-cell-indices-and-reticulocyte-parameters-for-carrier-screening-of-various-thalassemia-syndromes
#10
Orathai Tangvarasittichai, Nares Poonanan, Surapon Tangvarasittichai
Primary screening for thalassemia carriers usually involves an accurate blood count using an automated blood cell analyzer. We analyzed the red cell and reticulocyte parameters from 200 samples of various types of thalassemias and identified the discrimination criteria for differential diagnosis. These were separated into four groups based on genotypes. These groups included α-thalassemia, β-thalassemia, β-thalassemia with Hb E and Hb E trait, which are the important target of thalassemia screening. To compare the effectiveness of the screening strategies, seven selected screening tools were compared, including MCV alone (cutoff <80 fL); MRV alone (cutoff <100 fL); SD-C-NR alone (cutoff >28...
March 2017: Indian Journal of Clinical Biochemistry: IJCB
https://www.readbyqxmd.com/read/28135323/human-cord-blood-and-bone-marrow-cd34-cells-generate-macrophages-that-support-erythroid-islands
#11
Eyayu Belay, Brian J Hayes, C Anthony Blau, Beverly Torok-Storb
Recently, we developed a small molecule responsive hyperactive Mpl-based Cell Growth Switch (CGS) that drives erythropoiesis associated with macrophages in the absence of exogenous cytokines. Here, we compare the physical, cellular and molecular interaction between the macrophages and erythroid cells in CGS expanded CD34+ cells harvested from cord blood, marrow or G-CSF-mobilized peripheral blood. Results indicated that macrophage based erythroid islands could be generated from cord blood and marrow CD34+ cells but not from G-CSF-mobilized CD34+ cells...
2017: PloS One
https://www.readbyqxmd.com/read/28120415/immature-platelets-do-not-reliably-predict-platelet-recovery-in-patients-with-intensive-chemotherapy-or-stem-cell-transplantation
#12
L Meintker, J D Fritsch, J Ringwald, S W Krause
BACKGROUND AND OBJECTIVES: It has been reported that immature platelets may indicate imminent platelet (PLT) recovery following intensive chemotherapy and/or haematopoietic stem cell transplantation with the perspective to withhold unnecessary transfusions. The objective of our prospective study was to test immature PLTs as measured by two haematology analysers to predict PLT recovery. MATERIAL AND METHODS: This study monitored 51 courses of severe thrombocytopenia after chemotherapy in 35 adult haematology/oncology patients starting at a PLT count <70 × 10(9) /l until PLT recovery (>20 × 10(9) /l) or discharge from hospital...
January 25, 2017: Vox Sanguinis
https://www.readbyqxmd.com/read/28104703/risk-factors-for-mortality-in-adult-patients-with-sickle-cell-disease-a-meta-analysis-of-studies-in-north-america-and-europe
#13
Poulami Maitra, Melissa Caughey, Laura Robinson, Payal C Desai, Susan Jones, Mehdi Nouraie, Mark T Gladwin, Alan Hinderliter, Jianwen Cai, Kenneth I Ataga
Although recent studies show an improved survival of children with sickle cell disease in the US and Europe, mortality remains high for adult patients. This study was conducted to evaluate the factors associated with mortality in adult patients following the approval of hydroxyurea. We first evaluated the association between selected variables and mortality at an academic center (UNC cohort). Data sources were then searched for publications from 1998 to June, 2016, with meta-analysis of eligible studies conducted in North America and Europe to evaluate the associations of selected variables with mortality in adult patients...
January 19, 2017: Haematologica
https://www.readbyqxmd.com/read/28099399/the-association-of-serum-25-hydroxyvitamin-d-with-biomarkers-of-hemolysis-in-pediatric-patients-with-sickle-cell-disease
#14
Samuel A Adegoke, Josefina A P Braga, Adekunle D Adekile, Maria S Figueiredo
Although vitamin D deficiency (VDD) has been linked to anemia among sickle cell disease (SCD), its relationship with hemolysis is unclear. Serum 25-hydroxyvitamin D and biomarkers of hemolysis (hemoglobin [Hb]/hematocrit, reticulocyte percentage, absolute reticulocyte, and lactate dehydrogenase [LDH] levels) in 36 hydroxyurea-naive SCD children were quantified. Correlations were significantly positive with Hb/hematocrit (r=0.40, P=0.017; r=0.45, P=0.006, respectively); inverse with reticulocyte percentage, absolute reticulocyte, and LDH (r=-0...
January 17, 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28098787/pathological-impairment-cell-cycle-arrest-and-apoptosis-of-thymus-and-bursa-of-fabricius-induced-by-aflatoxin-contaminated-corn-in-broilers
#15
Xi Peng, Shiping Bai, Xuemei Ding, Keying Zhang
This study aimed to evaluate the comparative effects of aflatoxin-contaminated corn on the thymus and bursa of Fabricius (BF) in chickens by detecting histopathological lesions, cell cycle phase distribution and apoptosis. A total of 900 COBB500 male broilers were randomly allocated into five groups. The experiment lasted for six weeks and the five dietary treatments consisted of uncontaminated corn (control), 25% contaminated corn, 50% contaminated corn, 75% contaminated corn and 100% contaminated corn groups...
January 13, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28096331/one-step-design-of-a-stable-variant-of-the-malaria-invasion-protein-rh5-for-use-as-a-vaccine-immunogen
#16
Ivan Campeotto, Adi Goldenzweig, Jack Davey, Lea Barfod, Jennifer M Marshall, Sarah E Silk, Katherine E Wright, Simon J Draper, Matthew K Higgins, Sarel J Fleishman
Many promising vaccine candidates from pathogenic viruses, bacteria, and parasites are unstable and cannot be produced cheaply for clinical use. For instance, Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) is essential for erythrocyte invasion, is highly conserved among field isolates, and elicits antibodies that neutralize in vitro and protect in an animal model, making it a leading malaria vaccine candidate. However, functional RH5 is only expressible in eukaryotic systems and exhibits moderate temperature tolerance, limiting its usefulness in hot and low-income countries where malaria prevails...
January 31, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28095473/the-asymmetric-cell-division-regulators-par3-scribble-and-pins-gpsm2-are-not-essential-for-erythroid-development-or-enucleation
#17
Christina B Wölwer, Nathan Gödde, Luke B Pase, Imogen A Elsum, Krystle Y B Lim, Faruk Sacirbegovic, Carl R Walkley, Sarah Ellis, Shigeo Ohno, Fumio Matsuzaki, Sarah M Russell, Patrick O Humbert
Erythroid enucleation is the process by which the future red blood cell disposes of its nucleus prior to entering the blood stream. This key event during red blood cell development has been likened to an asymmetric cell division (ACD), by which the enucleating erythroblast divides into two very different daughter cells of alternate molecular composition, a nucleated cell that will be removed by associated macrophages, and the reticulocyte that will mature to the definitive erythrocyte. Here we investigated gene expression of members of the Par, Scribble and Pins/Gpsm2 asymmetric cell division complexes in erythroid cells, and functionally tested their role in erythroid enucleation in vivo and ex vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28089659/corrigendum-to-unambiguous-determination-of-plasmodium-vivax-reticulocyte-invasion-by-flow-cytometry-int-j-parasitol-46-2016-31-39
#18
Jee-Sun Cho, Bruce Russell, Varakorn Kosaisavee, Rou Zhang, Yves Colin, Olivier Bertrand, Rajesh Chandramohanadas, Cindy S Chu, Francois Nosten, Laurent Renia, Benoit Malleret
No abstract text is available yet for this article.
January 13, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28088966/-delayed-hematologic-response-to-immunosuppressive-therapy-in-severe-aplastic-anemia
#19
Y Yang, W R Yang, Z J Wu, X Zhao, L Zhang, L P Jing, K Zhou, Y Li, G X Peng, Y Li, J P Li, L Song, L Ye, H H Fan, F K Zhang
Objective: To explore the characteristics of delayed hematologic response in very/severe aplastic anemia (V/SAA) patients who were treated with immunosuppressive treatment (IST) as first-line approach, and investigate the rationality of early salvage treatment in refractory patients. Methods: The data of V/SAA patients front-line treated with IST were retrospectively analyzed. Delayed response was defined as acquiring hematologic response between 6 and 12 months after 1 course of IST. The clinical as well as hematologic characteristics of the delayed responded patients were investigated...
December 14, 2016: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/28078434/a-retrospective-open-label-uncontrolled-study-of-epoetin-zeta-on-the-treatment-of-chemotherapy-induced-anemia-in-solid-tumors
#20
Constantinos E Alifieris, Kyriakos Orfanakos, Aristina Papanota, George P Stathopoulos, Nikolaos Sitaras, Dimitrios T Trafalis
PURPOSE: This is a single-center uncontrolled retrospective study to evaluate the efficacy and safety of the biosimilar epoetin zeta after approval in chemotherapy-induced anemia (CIA). METHODS: Patients screened were >18 years old suffering from solid malignancies and CIA with Hg ≤10 or <11 g/dl if symptomatic anemia. Patients had measurable disease by TNM and Eastern Cooperative Oncology Group (ECOG). Patients were treated for at least 12 weeks and the primary endpoint was to determine the incidence of blood transfusions, and secondarily, the overall safety and efficacy defined as ≥1 g/dl rise in Hb concentration or ≥40,000 cells/μl rise in reticulocyte count...
January 11, 2017: Journal of Cancer Research and Clinical Oncology
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