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leishmania parasites

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https://www.readbyqxmd.com/read/28227369/hybrid-segmentation-of-fluorescent-leschmania-infected-images-using-a-watersched-and-combined-region-merging-based-method
#1
F Ouertani, H Amiri, J Bettaib, R Yazidi, A Ben Salah, F Ouertani, H Amiri, J Bettaib, R Yazidi, A Ben Salah, A Ben Salah, H Amiri, J Bettaib, R Yazidi, F Ouertani
Automatic parasite segmentation in fluorescent images is of high importance as it serves as an easier and faster tool for detecting and counting parasites in each focus. In this paper we present a hybrid segmentation for the Promastigote form of Leishmania parasites in Indirect Immunofluorescence (IIF) images, combining edge and region-based techniques through the morphological algorithm of watershed. The proposed approach deals first with a pre-processing step to correct illumination non-uniformities in the fluorescence Leishmania-infected images before performing the initial segmentation by means of the watershed algorithm...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28224222/immunopathological-characterization-of-human-cutaneous-leishmaniasis-lesions-caused-by-leishmania-viannia-spp-in-amazonian-brazil
#2
Cláudia Maria Castro Gomes, Maria Gloria Teixeira Sousa, Joyce Prieto Bezerra Menezes, Marliane Campos Batista, Ana Carolina Stocco Lima, Walter Belda, Daniel Bradshaw, Monica Elinor Alves Gama, Márcia Dalastra Laurenti, Fernando Tobias Silveira, Carlos Eduardo Pereira Corbett
American cutaneous leishmaniasis (ACL) is a chronic infectious disease caused by different protozoan species of Leishmania, and it is endemic in both tropical and subtropical countries. Using immunohistochemistry, we investigate the density of CD68(+), lysozyme(+), CD1a(+), factor XIIIa(+), CD4(+), CD8(+), CD56(+), interferon (IFN)-γ(+), and inducible NO synthase (iNOS(+)) cells. These cells were analyzed from 22 biopsy samples obtained from the lesions of ACL patients, whose infection was caused by Leishmania (Viannia) spp...
February 21, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28219639/leishmania-l-amazonensis-induces-hyperalgesia-in-balb-c-mice-contribution-of-endogenous-spinal-cord-tnf%C3%AE-and-nf%C3%AE%C2%BAb-activation
#3
Sergio M Borghi, Victor Fattori, Kenji W Ruiz-Miyazawa, Milena M Miranda-Sapla, Rúbia Casagrande, Phileno Pinge-Filho, Wander R Pavanelli, Waldiceu A Verri
: Cutaneous leishmaniasis (CL) is the most common form of the leishmaniasis in humans. Ulcerative painless skin lesions are predominant clinical features of CL. Wider data indicate pain accompanies human leishmaniasis, out with areas of painless ulcerative lesions per se. In rodents, Leishmania (L.) major infection induces nociceptive behaviors that correlate with peripheral cytokine levels. However, the role of the spinal cord in pain processing after Leishmania infection has not been investigated...
February 17, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28216511/global-kinetoplastea-phylogeny-inferred-from-a-large-scale-multigene-alignment-including-parasitic-species-for-better-understanding-transitions-from-a-free-living-to-a-parasitic-lifestyle
#4
Euki Yazaki, Sohta A Ishikawa, Keitaro Kume, Akira Kumagai, Takashi Kamaishi, Goro Tanifuji, Tetsuo Hashimoto, Yuji Inagaki
All members of the order Trypanosomatida known to date are parasites that are most likely descendants of a free-living ancestor. Trypanosomatids are an excellent model to assess the transition from a free-living to a parasitic lifestyle, because a large amount of experimental data has been accumulated for well-studied members that are harmful to humans and livestock (Trypanosoma spp. and Leishmania spp.). However, recent advances in our understanding of the diversity of trypanosomatids and their close relatives (i...
February 20, 2017: Genes & Genetic Systems
https://www.readbyqxmd.com/read/28214869/effects-of-antimalarial-tafenoquine-on-blood-platelet-activity-and-survival
#5
Hang Cao, Rosi Bissinger, Anja T Umbach, Abdulla Al Mamun Bhuyan, Florian Lang, Meinrad Gawaz
BACKGROUND/AIMS: The 8-aminoquinoline tafenoquine has been shown to be effective against Plasmodia, Leishmania and Trypanosoma. The substance is at least in part effective by triggering apoptosis of the parasites. Moreover, tafenoquine has been shown to trigger eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. The effect of tafenoquine on eryptosis is in part due to stimulation of Ca2+ entry and oxidative stress...
January 26, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28210511/unusual-presentation-of-cutaneous-leishmaniasis-ocular-leishmaniasis
#6
Masoud Doroodgar, Moein Doroodgar, Abbas Doroodgar
The leishmaniases are parasitic diseases that are transmitted to humans by infected female sandflies. Cutaneous leishmaniasis (CL) is one of 3 main forms of the disease. CL is the most common form of the disease and is endemic in many urban and rural parts of Iran and usually caused by two species of Leishmania: L. major and L. tropica. We report a case of unusual leishmaniasis with 25 lesions on exposed parts of the body and right eyelid involvement (ocular leishmaniasis). The patient was a 75-year-old male farmer referred to health care center in Aran va Bidgol city...
2017: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/28208616/immunoinformatics-features-linked-to-leishmania-vaccine-development-data-integration-of-experimental-and-in-silico-studies
#7
Rory C F Brito, Frederico G Guimarães, João P L Velloso, Rodrigo Corrêa-Oliveira, Jeronimo C Ruiz, Alexandre B Reis, Daniela M Resende
Leishmaniasis is a wide-spectrum disease caused by parasites from Leishmania genus. There is no human vaccine available and it is considered by many studies as apotential effective tool for disease control. To discover novel antigens, computational programs have been used in reverse vaccinology strategies. In this work, we developed a validation antigen approach that integrates prediction of B and T cell epitopes, analysis of Protein-Protein Interaction (PPI) networks and metabolic pathways. We selected twenty candidate proteins from Leishmania tested in murine model, with experimental outcome published in the literature...
February 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28207936/th17-cells-and-neutrophils-close-collaborators-in-chronic-leishmania-mexicana-infections-leading-to-disease-severity
#8
Claudia Patricia Pedraza-Zamora, José Delgado-Domínguez, Jaime Zamora-Chimal, Ingeborg Becker
Cutaneous leishmaniasis caused by Leishmania mexicana is associated with an important inflammatory response. We here analyzed the kinetics of Th17 cells and neutrophils in earlobe lesions caused by Leishmania mexicana throughout 90 days of disease progresion in susceptible BALB/c and semi-resistant C57BL/6 mice infected with 1x10(5) Leishmania mexicana promastigotes. Cells in the lesions were extracted and quantified by flow cytometry, whereas their distribution in the tissues in relation to the parasites was analyzed by immunohistochemistry...
February 16, 2017: Parasite Immunology
https://www.readbyqxmd.com/read/28202044/the-history-of-leishmaniasis
#9
REVIEW
Dietmar Steverding
In this review article the history of leishmaniasis is discussed regarding the origin of the genus Leishmania in the Mesozoic era and its subsequent geographical distribution, initial evidence of the disease in ancient times, first accounts of the infection in the Middle Ages, and the discovery of Leishmania parasites as causative agents of leishmaniasis in modern times. With respect to the origin and dispersal of Leishmania parasites, the three currently debated hypotheses (Palaearctic, Neotropical and supercontinental origin, respectively) are presented...
February 15, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28192528/cd8-t-cell-cytotoxicity-mediates-pathology-in-the-skin-by-inflammasome-activation-and-il-1%C3%AE-production
#10
Fernanda O Novais, Augusto M Carvalho, Megan L Clark, Lucas P Carvalho, Daniel P Beiting, Igor E Brodsky, Edgar M Carvalho, Phillip Scott
Deregulated CD8+ T cell cytotoxicity plays a central role in enhancing disease severity in several conditions. However, we have little understanding of the mechanisms by which immunopathology develops as a consequence of cytotoxicity. Using murine models of inflammation induced by the protozoan parasite leishmania, and data obtained from patients with cutaneous leishmaniasis, we uncovered a previously unrecognized role for NLRP3 inflammasome activation and IL-1β release as a detrimental consequence of CD8+ T cell-mediated cytotoxicity, ultimately resulting in chronic inflammation...
February 13, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28188871/cloning-characterization-and-subcellular-localization-of-nuclear-lim-interactor-interacting-factor-gene-from-leishmania-donovani
#11
R Ravinder, N Goyal
LIM domains are zinc-binding motifs that mediate protein-protein interactions and are found in a wide variety of cytoplasmic and nuclear proteins. The nuclear LIM domain family members have a number of different functions including transcription factors, gene regulation, cell fate determination, organization of the cytoskeleton and tumour formation exerting their function through various LIM domain interacting protein partners/cofactors. Nuclear LIM domain interacting proteins/factors have not been reported in any protozoan parasites including Leishmania...
February 7, 2017: Gene
https://www.readbyqxmd.com/read/28187163/enhanced-survival-of-leishmania-major-in-neutrophil-granulocytes-in-the-presence-of-apoptotic-cells
#12
Natallia Salei, Lars Hellberg, Jörg Köhl, Tamás Laskay
Neutrophil granulocytes are the first leukocytes that encounter and phagocytose Leishmania major (L. major) parasites in the infected skin. The parasites can nonetheless survive within neutrophils. However, the mechanisms enabling the survival of Leishmania within neutrophils are still elusive. Previous findings indicated that human neutrophils can engulf apoptotic cells. Since apoptotic neutrophils are abundant in infected tissues, we hypothesized that the uptake of apoptotic cells results in diminished anti-leishmanial activity and, consequently, contributes to enhanced survival of the parasites at the site of infection...
2017: PloS One
https://www.readbyqxmd.com/read/28182549/putative-drug-and-vaccine-target-identification-in-leishmania-donovani-membrane-proteins-using-na%C3%A3-ve-bayes-probabilistic-classifier
#13
Arvind Kumar Sinha, Pradeep Singh, Anand Prakash, Dharm Pal, Anuradha Dube, Awanish Kumar
Predicting the role of protein is one of the most challenging problems. There are few approaches available for the prediction of role of unknown protein in terms of drug target or vaccine candidate. We propose here Naïve Bayes probabilistic classifier, a promising method for reliable predictions. This method is tested on the proteins identified in our mass spectrometry based membrane protemics study of Leishmania donovani parasite that causes a fatal disease (Visceral Leishmaniasis) in humans all around the world...
January 2017: IEEE/ACM Transactions on Computational Biology and Bioinformatics
https://www.readbyqxmd.com/read/28182432/the-interaction-of-kmp-11-with-phospholipid-membranes-and-its-implications-in-leishmaniasis-the-effects-of-single-tryptophan-mutations-and-cholesterol
#14
Achinta Sannigrahi, Pabitra Maity, Sanat Karmakar, Krishnananda Chattopadhyay
KMP-11 is a small protein, which is believed to control overall bilayer pressure of leishmania parasite. Recent results suggested that membrane binding and the presence of cholesterol affect the efficacy of leishmanial infection, in which KMP-11 play an important role. Nevertheless, there exists no systematic study of membrane interaction with KMP-11 either in the absence or presence of cholesterol. In this paper, we investigated the interaction between KMP-11 and phospholipid membranes, using an unsatuarated (PC 18:1) 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and a saturated (PC 12:0) 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) lipid, as membrane mimics...
February 9, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28179142/rna-seq-in-kinetoplastids-a-powerful-tool-for-the-understanding-of-the-biology-and-host-pathogen-interactions
#15
REVIEW
Luz Helena Patino, Juan David Ramírez
The kinetoplastids include a large number of parasites responsible for serious diseases in humans and animals (Leishmania and Trypanosoma brucei) considered endemic in several regions of the world. These parasites are characterized by digenetic life cycles that undergo morphological and genetic changes that allow them to adapt to different microenvironments on their vertebrates and invertebrates hosts. Recent advances in ´omics´ technology, specifically transcriptomics have allowed to reveal aspects associated with such molecular changes...
February 4, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28179112/glucose-6-phosphate-dehydrogenase-and-trypanothione-reductase-interaction-protects-leishmania-donovani-from-metalloid-mediated-oxidative-stress
#16
Ayan Kumar Ghosh, Savita Saini, Sushmita Das, Abhishek Mandal, Abul Hasan Sardar, Md Yousuf Ansari, Kumar Abhishek, Ajay Kumar, Ruby Singh, Sudha Verma, Asif Equbal, Vahab Ali, Pradeep Das
Exploration of metabolons as viable drug target is rare in kinetoplastid biology. Here we present a novel protein-protein interaction among Glucose-6-phosphate dehydrogenase (LdG6PDH) and Trypanothione reductase (LdTryR) of Leishmania donovani displaying interconnection between central glucose metabolism and thiol metabolism of this parasite. Digitonin fractionation patterns observed through immunoblotting indicated localisation of both LdG6PDH and LdTryR in cytosol. In-silico and in-vitro interaction observed by size exclusion chromatography, co-purification, pull-down assay and spectrofluorimetric analysis revealed LdG6PDH and LdTryR physically interact with each other in a NADPH dependent manner...
February 4, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28177050/achievement-of-constitutive-fluorescent-plexsy-egfp-leishmania-braziliensis-and-its-application-as-an-alternative-method-for-drug-screening-in-vitro
#17
Matheus Silva E Bastos, Luciana Ângelo de Souza, Thiago Souza Onofre, Abelardo Silva, Márcia Rogéria de Almeida, Gustavo Costa Bressan, Juliana Lopes Rangel Fietto
BACKGROUND: Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE: Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS: L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays...
February 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28177049/n-acetyl-cysteine-inhibits-liver-oxidative-stress-markers-in-balb-c-mice-infected-with-leishmania-amazonensis
#18
Juciano Gasparotto, Alice Kunzler, Mario Roberto Senger, Celeste da Silva Freitas de Souza, Salvatore Giovanni de Simone, Rafael Calixto Bortolin, Nauana Somensi, Felipe Dal-Pizzol, José Claudio Fonseca Moreira, Ana Lúcia Abreu-Silva, Kátia da Silva Calabrese, Floriano Paes Silva, Daniel Pens Gelain
BACKGROUND: Leishmaniasis is a parasitosis caused by several species of the genus Leishmania. These parasites present high resistance against oxidative stress generated by inflammatory cells. OBJECTIVES: To investigate oxidative stress and molecular inflammatory markers in BALB/c mice infected with L. amazonensis and the effect of antioxidant treatment on these parameters. METHODS: Four months after infection, oxidative and inflammatory parameters of liver, kidneys, spleen, heart and lungs from BALB/c mice were assessed...
February 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28177041/leishmania-in-synanthropic-rodents-rattus-rattus-new-evidence-for-the-urbanization-of-leishmania-leishmania-amazonensis
#19
Eloiza Teles Caldart, Roberta Lemos Freire, Fernanda Pinto Ferreira, Bruno Bergamo Ruffolo, Mônica Raquel Sbeghen, Marcelle Mareze, João Luis Garcia, Regina Mitsuka-Breganó, Italmar Teodorico Navarro
This study aimed to detect parasites from Leishmania genus, to determine the prevalence of anti-Leishmania spp. antibodies, to identify circulating species of the parasite, and to determine epidemiological variables associated with infection in rats caught in urban area of Londrina, Paraná, Brazil. Animal capture was carried out from May to December 2006, serological and molecular methods were performed. DNA was extracted from total blood, and nested-PCR, targeting SSu rRNA from Leishmania genus, was performed in triplicate...
February 6, 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/28174102/copper-salisylaldoxime-cusal-imparts-protective-efficacy-against-visceral-leishmaniasis-by-targeting-leishmania-donovani-topoisomerase-ib
#20
Manoj Kumar Singh, Siddhartha Kumar Bhaumik, Subir Karmakar, Joydeep Paul, Sudeshna Sawoo, Hemanta K Majumder, Amit Roy
In vitro and in vivo anti-leishmanial efficacy of copper salisylaldoxime (CuSAL), a transition metal complex, was evaluated and the underlying mechanism was studied. In vitro studies revealed that 30 μM of CuSAL causes 96% reduction in parasite burden in infected macrophages. CuSAL is least toxic in host cells. A dose of 5 mg/kg bodyweight per mice on alternate days (5 doses) gives ∼97% protection in both liver and spleen. Moreover, CuSAL potentially inhibits the catalytic activity of LdTOPILS and causes apoptosis of Leishmania parasites through induction of intracellular ROS generation and activation of caspase-like proteases...
February 4, 2017: Experimental Parasitology
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