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https://www.readbyqxmd.com/read/28438716/yap1-negatively-regulates-chondrocyte-differentiation-partly-by-activating-the-%C3%AE-catenin-signaling-pathway
#1
Beining Yang, Hualing Sun, Fangfang Song, Miao Yu, Yanru Wu, Jiawei Wang
YAP1 (Yes-associated protein 1) transcriptional coactivator is a downstream gene of the Hippo signaling pathway, which controls cell proliferation and differentiation. YAP1 plays a significant role in the regulation of cartilage and bone development. However, the molecular mechanism by which YAP1 regulates chondrocyte differentiation remains to be elucidated. Immunofluorescent staining was used to visualize the localization of YAP1 expression in the mouse chondroprogenitor ATDC5 cell line. ATDC5 cells with lentivirus-vector-mediated YAP1 overexpression and knockdown were established...
April 21, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28423208/combined-activity-of-temozolomide-and-the-mtor-inhibitor-temsirolimus-in-metastatic-melanoma-involves-dkk1
#2
Heike Niessner, Corinna Kosnopfel, Tobias Sinnberg, Daniela Beck, Kathrin Krieg, Ines Wanke, Konstantinos Lasithiotakis, Michael Bonin, Claus Garbe, Friedegund Meier
The BRAFV600E inhibitor vemurafenib achieves remarkable clinical responses in patients with BRAF-mutant melanoma, but its effects are limited by the onset of drug resistance. In the case of resistance, chemotherapy can still be applied as second line therapy. However, it yields low response rates and strategies are urgently needed to potentiate its effects. In a previous study, we showed that the inhibition of the PI3K-AKT-mTOR pathway significantly increases sensitivity of melanoma cells to chemotherapeutic drugs (1)...
April 19, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28396299/plasma-cytokine-profiling-to-predict-susceptibility-to-acute-mountain-sickness
#3
Hui Lu, Rong Wang, Wenbin Li, Hua Xie, Chang Wang, Ying Hao, Yuhuan Sun, Zhengping Jia
Extensive studies have been performed on acute mountain sickness (AMS), but biomarkers predicting AMS are lacking. Presently, the mainstay methods to identify AMS biomarkers include proteomic and genetic methods at high altitudes or in hypoxic simulated chambers. In the present study, we compared plasma cytokine profiles between AMS-susceptible individuals and AMS-resistant individuals at low altitude by cytokine array analysis. In total, 75 differentially expressed cytokines were identified between AMS-susceptible individuals and AMS-resistant individuals, most involved in inflammation...
November 1, 2016: European Cytokine Network
https://www.readbyqxmd.com/read/28388723/high-sclerostin-and-dickkopf-1-dkk-1-serum-levels-in-children-and-adolescents-with-type-1-diabetes-mellitus
#4
Maria Felicia Faienza, Annamaria Ventura, Maurizio Delvecchio, Anna Fusillo, Laura Piacente, Gabriella Aceto, Graziana Colaianni, Silvia Colucci, Luciano Cavallo, Maria Grano, Giacomina Brunetti
Context: Childhood type 1 diabetes mellitus (T1DM) is associated with decreased bone mass. Sclerostin and dickkopf-1 (DKK-1) are Wnt inhibitors that regulate bone formation. Objective: To evaluate sclerostin and DKK-1 levels in T1DM children and to analyze the influence of glycemic control on bone health. Design and setting: Cross-sectional study conducted at a clinical research center. Participants: One hundred and six T1DM subjects (12...
April 1, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28384913/study-of-dickkopf-1-dkk-1-gene-expression-in-hepatocellular-carcinoma-patients
#5
Mona Watany, Rehab Badawi, Walaa Elkhalawany, Sherief Abd-Elsalam
INTRODUCTION: Hepatocellular Carcinoma (HCC) is the sixth most common cancer in the world. Dickkopf -1 (DKK-1) protein is a new biomarker used in conjunction with Alpha Fetoprotein (AFP) to differentiate HCC from "non-malignant" liver disease. DKK-1 is an inhibitor of Wnt/β-catenin signaling pathway which is involved in embryogenesis and has been implicated in tumorigenesis in many tissues. AIM: To investigate the level of DKK-1 gene expression in the peripheral blood of patients with HCC who had a history of Hepatitis C Virus (HCV) and schistosomal infections...
February 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28373995/dickkopf-1-is-a-biomarker-for-systemic-lupus-erythematosus-and-active-lupus-nephritis
#6
Jing Xue, Jiali Yang, Lijuan Yang, Shaolan Zhou, Chen Ji, Xuemei Wang, Nan Yu, Xiaoming Liu, Shuhong Chi
An early diagnosis of lupus nephritis (LN) has an important clinical implication in guiding treatments of systemic lupus erythematosus (SLE) in clinical settings. In this study, the concentrations of Wnt-3A, Frizzled-8 (FZD-8), and Dickkopf-1 (DKK-1) of Wnt signaling, as well as their diagnostic values for accessing LN, were evaluated by ELISA in sera and urine of 111 SLE patients (31 with LN and 80 without LN) and 70 healthy cohorts. Significantly more abundances of DKK-1 protein were determined in both of sera and urine of SLE patients compared to healthy cohorts (p < 0...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28369789/increased-il-20-and-il-24-target-osteoblasts-and-synovial-monocytes-in-spondyloarthritis
#7
Tue Wenzel Kragstrup, Morten Nørgaard Andersen, Berit Schiøttz-Christensen, Anne Grethe Jurik, Malene Hvid, Bent Deleuran
The pathogenesis of spondyloarthritis (SpA) involves activation of the innate immune system, inflammation and new bone formation. The two cytokines IL-20 and IL-24 have been shown to link innate immune activation and tissue homeostasis. We hypothesized that these two cytokines are secreted as part of activation of the innate immune system and affect bone homeostasis in SpA. IL-20 and IL-24 were measured in plasma from axial SpA patients (n=83). Peripheral SpA patients (n=16) were included for in vitro cell culture studies...
April 2, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28365691/dickkopf-related-protein-2-is-epigenetically-inactivated-and-suppresses-colorectal-cancer-growth-and-tumor-metastasis-by-antagonizing-wnt-%C3%AE-catenin-signaling
#8
Can Wang, Yujuan Yue, Bianfei Shao, Zhu Qiu, Junhao Mu, Jun Tang, Xiaofan Han, Tingxiu Xiang, Guosheng Ren
BACKGROUND/AIMS: Aberrant activation of the Wnt/β-catenin signaling pathway plays a key role in the pathogenesis of multiple tumors including digestive cancers. Recent studies have reported that Dickkopf-related protein 2 (DKK2) is epigenetically inactivated in numerous types of cancers and that its gene products exhibit tumor-suppressive properties. However, the biological functions and underlying molecular mechanisms of DKK2 in colon carcinoma remains obscure. METHODS: We examined the expression of DKK2 in colon tumor cell lines by RT-PCR and its promoter methylation status in colon tumor cell lines and primary tumors by methylation-specific PCR (MSP)...
March 30, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28358733/plasma-dickkopf-related-protein-1-an-antagonist-of-the-wnt-pathway-is-associated-with-hiv-associated-neurocognitive-impairment
#9
Chunjiang Yu, Melanie Seaton, Scott Letendre, Robert Heaton, Lena Al-Harthi
OBJECTIVE: DKK1 is a soluble antagonist of the Wnt pathway. It binds to and sequesters LRP5/6 away from Wnts. Because the Wnt pathway regulates synaptic transmission and plasticity, we hypothesized that increased DKK1 would increase the risk for neurocognitive impairment (NCI) in HIV+ individuals. We evaluated here the relationship between plasma DKK1 and global NCI. METHODS: Plasma samples and data from 41 HIV+ and 42 HIV- adults were obtained from the University of California, San Diego...
March 29, 2017: AIDS
https://www.readbyqxmd.com/read/28356933/dkk4-enhances-resistance-to-chemotherapeutics-5-fu-and-yn968d1-in-colorectal-cancer-cells
#10
Shengli He, Jie Shen, Nanhua Hu, Xuanfu Xu, Jin Li
Dickkopf-related protein 4 (DKK4) is a target of the β-catenin/transcription factor 4 complex in colorectal cancer. Previous research has demonstrated that its expression level may vary and has indicated that it may have a role in the development of resistance to chemotherapy in colorectal cancer cells. In the present study, DKK4 was over expressed in several colorectal cancer cell lines. The DKK4 over-expressing cell lines were screened using reverse transcription quantitative polymerase chain reaction analysis and western blotting...
February 2017: Oncology Letters
https://www.readbyqxmd.com/read/28356931/microrna-320a-downregulation-mediates-human-liver-cancer-cell-proliferation-through-the-wnt-%C3%AE-catenin-signaling-pathway
#11
Caicheng Lu, Zengwei Liao, Minxian Cai, Guirong Zhang
MicroRNAs (miRs) have emerged as key epigenetic regulators involved in cancer progression. miR-320a has been demonstrated to be a novel tumor suppressive microRNA in several types of cancers. In the present study, the role of miR-320a in human hepatocellular carcinoma (HCC) was investigated. The expression levels of miR-320a and messenger RNA were determined by reverse transcription-quantitative polymerase chain reaction, while cell cycle and cell apoptosis were analyzed by flow cytometry. The cell proliferative ability was determined by Cell Counting Kit-8 assay and colony formation assay...
February 2017: Oncology Letters
https://www.readbyqxmd.com/read/28352232/dickkopf-3-upregulates-vegf-in-cultured-human-endothelial-cells-by-activating-activin-receptor-like-kinase-1-alk1-pathway
#12
Carla L Busceti, Simona Marchitti, Franca Bianchi, Paola Di Pietro, Barbara Riozzi, Rosita Stanzione, Milena Cannella, Giuseppe Battaglia, Valeria Bruno, Massimo Volpe, Francesco Fornai, Ferdinando Nicoletti, Speranza Rubattu
Dkk-3 is a member of the dickkopf protein family of secreted inhibitors of the Wnt pathway, which has been shown to enhance angiogenesis. The mechanism underlying this effect is currently unknown. Here, we used cultured HUVECs to study the involvement of the TGF-β and VEGF on the angiogenic effect of Dkk-3. Addition of hrDkk-3 peptide (1 or 10 ng/ml) to HUVECs for 6 or 12 h enhanced the intracellular and extracellular VEGF protein levels, as assessed by RTPCR, immunoblotting, immunocytochemistry and ELISA...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28350100/overexpression-of-mir-203-increases-the-sensitivity-of-nsclc-a549-h460-cell-lines-to-cisplatin-by-targeting-dickkopf-1
#13
Ruirui Cheng, Chunya Lu, Guojun Zhang, Guowei Zhang, Guoqiang Zhao
The number of new lung cancer cases diagnosed yearly is high, and the mortality rate has not substantially declined. Non-small cell lung cancer (NSCLC) is the most common type of lung cancer, and adenocarcinoma accounts for the largest proportion of NSCLC. Currently, platinum-based combined chemotherapy, particularly cisplatin (DDP) is still the main form of treatment for advanced NSCLC. However, cisplatin resistance often occurs in patients who receive chemotherapy. Previous studies offer various explanations for how miRNAs affect cisplatin resistance, but the underlying mechanism remains largely unknown...
April 2017: Oncology Reports
https://www.readbyqxmd.com/read/28346008/wnt-%C3%AE-catenin-signaling-is-required-for-distraction-osteogenesis-in-rats
#14
Xuemei Wang, En Luo, Ruiye Bi, Bin Ye, Jing Hu, Shujuan Zou
OVERVIEW: The Wnt signaling pathway plays crucial roles in embryonic skeletal development and postnatal bone regeneration. However, mechanisms of Wnt signaling functioning in distraction osteogenesis (DO) haven't been well characterized. MATERIALS AND METHODS: We established a DO model using Sprague-Dawley rat tibia. And a Wnt signaling blocking agent, recombinant rat Dickkopf-related protein 1 (rrDkk1), was locally applied in the distracted gap to study the role of Wnt signaling during DO process...
March 1, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28342972/remodeling-the-th1-polarized-systemic-environment-contributes-to-neurogenesis-and-cognitive-function-via-the-wnt7a-pathway-in-neonatal-mice
#15
YunLong Xu, Fen He, FangFang Qi, Guangqi Yang, FuXiang Zheng, JunHua Yang, Xiao Wang, JunXiu Liu, JunTao Zou
Neonatal Bacillus Calmette-Guérin (BCG) vaccination results in a positive effect on hippocampal neurogenesis and cognition. Serum cytokines are considered to be the chief culprit. In this study, serum from BCG-treated mice was identified as Th1 polarized serum. The serum showed an increased ratio of IFN-γ to IL-4 and decreased levels of TNF-α and IL-6. After Th1 polarized serum was injected intraperitoneally into postnatal mice, the levels of cytokines and ratio of IFN-γ to IL-4 in the serum and hippocampus of postnatal mice showed a similar alteration as those in Th1 polarized serum...
March 22, 2017: Neurobiology of Learning and Memory
https://www.readbyqxmd.com/read/28337453/mir-373-3p-targets-dkk1-to-promote-emt-induced-metastasis-via-the-wnt-%C3%AE-catenin-pathway-in-tongue-squamous-cell-carcinoma
#16
Junquan Weng, Hui Zhang, Cheng Wang, Jianfeng Liang, Guanhui Chen, Wenqing Li, Haikuo Tang, Jinsong Hou
MicroRNAs (miRNAs) regulate gene expression and at the same time mediate tumorigenesis. miR-373-3p has diverse effects in tumors, but its role in tongue squamous cell carcinoma (TSCC) remains unknown. The purpose of this study is to determine the function of miR-373-3p in the progression of TSCC. Our results brought to light that miR-373-3p is markedly upregulated in clinical TSCC tissues compared with paired adjacent normal tissues and has significant correlation with a more aggressive TSCC phenotype in patients...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28332354/cyclized-oligopeptide-targeting-lrp5-6-dkk1-interaction-reduces-the-growth-of-tumor-burden-in-a-multiple-myeloma-mouse-model
#17
Bo Mi Park, Eun Jin Kim, Hee Jin Nam, Dongdong Zhang, Chu Hyun Bae, Myeongmo Kang, Heeyoun Kim, Weontae Lee, Bjarne Bogen, Sung Kil Lim
PURPOSE: Dickkopf 1 (DKK1) has been extensively investigated in mouse models of multiple myeloma, which results in osteolytic bone lesions. Elevated DKK1 levels in bone marrow plasma and serum inhibit the differentiation of osteoblast precursors. Present pharmaceutical approaches to target bone lesions are limited to antiresorptive agents. In this study, we developed a cyclized oligopeptide against DKK1-low density lipoprotein receptor-related protein (LRP) 5/6 interaction and tested the effects of the oligopeptide on tumor burden...
May 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/28303752/bone-morphogenetic-proteins-and-dickkopf-1-in-ankylosing-spondylitis
#18
H-T Liao, Y-F Lin, C-Y Tsai, T-C Chou
OBJECTIVES: To determine bone morphogenetic proteins (BMPs) and Dickkopf homologue-1 (Dkk-1) levels in ankylosing spondylitis (AS). METHOD: Serum BMPs and Dkk-1 were measured in 72 AS patients and 30 healthy controls. For AS patients, we recorded the demographic data, disease activity, functional index, and global assessment with questionnaires, and image changes with roentgenography. We also measured human leucocyte antigen-B27 and systemic inflammatory reactants...
March 17, 2017: Scandinavian Journal of Rheumatology
https://www.readbyqxmd.com/read/28299863/dickkopf-3-attenuates-xanthine-dehydrogenase-expression-to-prevent-oxidative-stress-induced-apoptosis
#19
Shuang Qui, Junko Kano, Masayuki Noguchi
Dickkopf (DKK) 3 is a DKK glycoprotein family member that controls cell fate during embryogenesis and exerts opposing effects on survival in a cell type-dependent manner; however, the mechanisms governing its pro-apoptosis versus pro-survival functions remain unclear. Here, we investigated DKK3 function in Li21 hepatoma cells and tPH5CH immortalized hepatocytes. DKK3 knockdown by siRNA resulted in reactive oxygen species accumulation and subsequent apoptosis, which were abrogated by administration of the antioxidant N-acetyl-cysteine...
April 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/28273073/src-2-mediated-coactivation-of-anti-tumorigenic-target-genes-suppresses-myc-induced-liver-cancer
#20
Shruthy Suresh, Deniz Durakoglugil, Xiaorong Zhou, Bokai Zhu, Sarah A Comerford, Chao Xing, Xian-Jin Xie, Brian York, Kathryn A O'Donnell
Hepatocellular carcinoma (HCC) is the fifth most common solid tumor in the world and the third leading cause of cancer-associated deaths. A Sleeping Beauty-mediated transposon mutagenesis screen previously identified mutations that cooperate with MYC to accelerate liver tumorigenesis. This revealed a tumor suppressor role for Steroid Receptor Coactivator 2/Nuclear Receptor Coactivator 2 (Src-2/Ncoa2) in liver cancer. In contrast, SRC-2 promotes survival and metastasis in prostate cancer cells, suggesting a tissue-specific and context-dependent role for SRC-2 in tumorigenesis...
March 2017: PLoS Genetics
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