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high frequency ultrasound

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https://www.readbyqxmd.com/read/28723853/high-frequency-ultrasound-description-of-sacral-tissue-characteristics-in-healthy-adults
#1
Ruth S Burk, Christine M Schubert, Anathea Pepperl, Mary Jo Grap
OBJECTIVES: The purpose of this study was to describe selected sacral tissue characteristics in a convenience sample of healthy volunteer subjects. DESIGN: Descriptive. SAMPLE AND SETTING: Fifty healthy volunteers in a clinical learning center in a school of nursing. METHODS: Sacral scans were obtained using a 20-MHz ultrasound scanning system in 3 positions: prone and 60° and 90° side-lying from the back. The images were analyzed by software in the ultrasound program using quantitative parameters of dermal thickness and density (dermal median intensity and derived number of low-echogenic pixels to total pixels [LEP:TP] ratio)...
July 18, 2017: Journal of Wound, Ostomy, and Continence Nursing
https://www.readbyqxmd.com/read/28722425/self-focused-alscn-film-ultrasound-transducer-for-individual-cell-manipulation
#2
Benpeng Zhu, Chunlong Fei, Chen Wang, Yuhang Zhu, Xiaofei Yang, Hairong Zheng, Qifa Zhou, K Kirk Shung
Precise cell positioning is indispensable in the fields of biophysics and cellular biology. Acoustic microbeam produced by a highly focused ultrasound transducer has recently been investigated for a particle or cell manipulation. By virtue of the relatively good piezoelectric property, Sc doped AlN film was introduced for a highly focused ultrasound transducer application. Using a sputtering approach, a self-focused AlScN film based device has been designed, fabricated, and characterized at a frequency of ∼230 MHz...
January 27, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28720900/low-frequency-ultrasound-induced-vegf-suppression-and-synergy-with-dendritic-cell-mediated-anti-tumor-immunity-in-murine-prostate-cancer-cells-in-vitro
#3
Wei Zhang, Wen-De Shou, Yan-Jun Xu, Wen-Kun Bai, Bing Hu
High tumor vascular endothelial growth factor (VEGF) levels are associated with poor treatment outcomes in prostate cancer (PCa), and immune deficiency in the PCa microenvironment, especially suppression of dendritic cell (DC) proliferation, has been confirmed. In this study, we (1) investigated whether VEGF participates in DC suppression in murine PCa cells (RM-1), (2) down-regulated VEGF expression using low-frequency ultrasound and microbubbles (UM), and (3) further explored any synergistic effect on immunological activation...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28718938/quality-control-of-ultrasound-for-fetal-biometry-results-from-the-intergrowth-21-st-project
#4
Angelo Cavallaro, Stephen T Ash, Raffaele Napolitano, Sikolia Wanyonyi, Eric O Ohuma, Malid Molloholli, Joyce Sande, Ippokratis Sarris, Christos Ioannou, Tess Norris, Vera Donadono, Maria Carvalho, Manorama Purwar, Fernando C Barros, Yasmin A Jaffer, Enrico Bertino, Ruyan Pang, Michael G Gravett, Laurent J Salomon, Julia Alison Noble, Douglas G Altman, Aris T Papageorghiou
OBJECTIVES: To assess a comprehensive package of ultrasound quality control in a large multicentre study of fetal growth - the Fetal Growth Longitudinal Study of the INTERGROWTH-21(st) Project. METHODS: We performed quality control (QC) measures on 20,313 ultrasound scan images taken prospectively from 4,321 fetuses at 14-41 weeks' gestation in eight geographical locations. At the time of each ultrasound examination, three fetal biometric variables were measured in triplicate on separately generated images: head circumference (HC), abdominal circumference (AC) and femur length (FL)...
July 18, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28715512/mouse-models-of-deep-vein-thrombosis
#5
T Schönfelder, S Jäckel, P Wenzel
The pathogenesis of venous thromboembolism (VTE) is still not completely understood. Experimental animals in which human deep vein thrombosis can be modeled are useful tools to investigate the pathogenesis of VTE. Besides the availability of transgenic and genetically modified mice, the use of high frequency ultrasound and intravital microscopy plays an important role in identifying thrombotic processes in mouse models. In this article, an overview about the application of various new technologies and existing mouse models is provided, and the impact of venous side branches on deep vein thrombosis in the mouse model is discussed...
2017: Gefässchirurgie: Zeitschrift Für Vaskuläre und Endovaskuläre Chirurgie
https://www.readbyqxmd.com/read/28711187/development-of-an-in-vitro-diaphragm-motion-reproduction-system
#6
Ai-Ho Liao, Ho-Chiao Chuang, Ming-Chih Shih, Hsiao-Yu Hsu, Der-Chi Tien, Chia-Chun Kuo, Shiu-Chen Jeng, Jeng-Fong Chiou
This study developed an in vitro diaphragm motion reproduction system (IVDMRS) based on noninvasive and real-time ultrasound imaging to track the internal displacement of the human diaphragm and diaphragm phantoms with a respiration simulation system (RSS). An ultrasound image tracking algorithm (UITA) was used to retrieve the displacement data of the tracking target and reproduce the diaphragm motion in real time using a red laser to irradiate the diaphragm phantom in vitro. This study also recorded the respiration patterns in 10 volunteers...
July 2017: Physica Medica: PM
https://www.readbyqxmd.com/read/28710752/ultrasound-imaging-of-apoptosis-spectroscopic-detection-of-dna-damage-effects-in-vivo
#7
Hadi Tadayyon, Mehrdad J Gangeh, Roxana Vlad, Michael C Kolios, Gregory J Czarnota
In this chapter, we describe two new methodologies: (1) application of high-frequency ultrasound spectroscopy for in vivo detection of cancer cell death in small animal models, and (2) extension of ultrasound spectroscopy to the lower frequency range (i.e., 1-10 MHz range) for the detection of cell death in vivo in preclinical and clinical settings. Experiments using tumor xenografts in mice and cancer treatments based on chemotherapy are described. Finally, we describe how one can detect cancer response to treatment in patients noninvasively early (within 1 week of treatment initiation) using low-frequency ultrasound spectroscopic imaging and advanced machine learning techniques...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28710751/ultrasound-imaging-of-dna-damage-effects-in-live-cultured-cells-and-in-brain-tissue
#8
Hadi Tadayyon, Mehrdad J Gangeh, Roxana Vlad, Michael C Kolios, Gregory J Czarnota
High-frequency ultrasound (>20 MHz) spectroscopy can be used to detect noninvasively DNA damage in cell samples in vitro, and in live tissue both ex vivo and in vivo. This chapter focuses on the former two aspects. Experimental evidence suggests that morphological changes that occur in cells undergoing apoptosis result in changes in frequency-dependent ultrasound backscatter. With advances in research, ultrasound spectroscopy is advancing the boundaries of fast, label-free, noninvasive DNA damage detection technology with potential use in personalized medicine and early therapy response monitoring...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28708551/indoor-airborne-ultrasonic-wireless-communication-using-ofdm-methods
#9
Wentao Jiang, William M D Wright
Concerns still exist over the safety of prolonged exposure to radio frequency (RF) wireless transmissions and there are also potential data security issues due to remote signal interception techniques such as Bluesniping. Airborne ultrasound may be used as an alternative to RF for indoor wireless communication systems for securely transmitting data over short ranges, as signals are difficult to intercept from outside the room. Two types of air-coupled capacitive ultrasonic transducer were used in the implementation of an indoor airborne wireless communication system...
July 11, 2017: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/28706248/acoustic-transfection-for-genomic-manipulation-of-single-cells-using-high-frequency-ultrasound
#10
Sangpil Yoon, Pengzhi Wang, Qin Peng, Yingxiao Wang, K Kirk Shung
Efficient intracellular delivery of biologically active macromolecules has been a challenging but important process for manipulating live cells for research and therapeutic purposes. There have been limited transfection techniques that can deliver multiple types of active molecules simultaneously into single-cells as well as different types of molecules into physically connected individual neighboring cells separately with high precision and low cytotoxicity. Here, a high frequency ultrasound-based remote intracellular delivery technique capable of delivery of multiple DNA plasmids, messenger RNAs, and recombinant proteins is developed to allow high spatiotemporal visualization and analysis of gene and protein expressions as well as single-cell gene editing using clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein-9 nuclease (Cas9), a method called acoustic-transfection...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28704403/genetic-variants-of-ptgs2-txa2r-and-txas1-are-associated-with-carotid-plaque-vulnerability-platelet-activation-and-txa2-levels-in-ischemic-stroke-patients
#11
Xingyang Yi, Jing Lin, Hua Luo, Chun Wang, Yingying Liu
Eicosanoids may play a role in ischemic stroke. However, the associations of variants in cyclooxygenase (COX) pathway genes and interaction among these variants with carotid plaque vulnerability are not fully understood. In present study, twelve variants in COX pathway genes were examined using matrix-assisted laser desorption ionization time-of-flight mass spectrometry method in 396 patients with ischemic stroke and 291 controls. Platelet aggregation, platelet-leukocyte aggregates, and urine 11-dehydrothromboxane B2 (11-dTxB2) were also measured...
2017: PloS One
https://www.readbyqxmd.com/read/28691450/ultrasound-imaging-of-oral-fibroma-a-case-report
#12
D Di Stasio, D Lauritano, R Paparella, R Franco, M Montella, R Serpico, A Lucchese
Oral mucosa is constantly under the influence of various internal and external stimuli, therefore it is interested by a range of developmental disorders, irritation, inflammation, and neoplastic conditions. Traumatic fibroma is one of these lesions. The aim of this work is to obtain an ultrasound characterization of a lip fibroma with a high frequency probe (8-18 MHz) as feasibility and experimental model of this non-invasive technique in the oral mucosa compared with its histo-pathological section. Ultrasound characterization of a fibroma in a 50-year-old patient in the right retro commissural area of mucosa was performed...
April 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28686673/focal-areas-of-increased-lipid-concentration-on-the-coating-of-microbubbles-during-short-tone-burst-ultrasound-insonification
#13
Klazina Kooiman, Tom van Rooij, Bin Qin, Frits Mastik, Hendrik J Vos, Michel Versluis, Alexander L Klibanov, Nico de Jong, Flordeliza S Villanueva, Xucai Chen
Acoustic behavior of lipid-coated microbubbles has been widely studied, which has led to several numerical microbubble dynamics models that incorporate lipid coating behavior, such as buckling and rupture. In this study we investigated the relationship between microbubble acoustic and lipid coating behavior on a nanosecond scale by using fluorescently labeled lipids. It is hypothesized that a local increased concentration of lipids, appearing as a focal area of increased fluorescence intensity (hot spot) in the fluorescence image, is related to buckling and folding of the lipid layer thereby highly influencing the microbubble acoustic behavior...
2017: PloS One
https://www.readbyqxmd.com/read/28679279/in-vivo-measurement-of-basilar-membrane-vibration-in-the-unopened-chinchilla-cochlea-using-high-frequency-ultrasound
#14
Thomas G Landry, Manohar L Bance, Jeffrey Leadbetter, Robert B Adamson, Jeremy A Brown
The basilar membrane and organ of Corti in the cochlea are essential for sound detection and frequency discrimination in normal hearing. There are currently no methods used for real-time high resolution clinical imaging or vibrometry of these structures. The ability to perform such imaging could aid in the diagnosis of some pathologies and advance understanding of the causes. It is demonstrated that high frequency ultrasound can be used to measure basilar membrane vibrations through the round window of chinchilla cochleas in vivo...
June 2017: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/28679248/ultrasound-field-characterization-using-synthetic-schlieren-tomography
#15
Aki Pulkkinen, Jarkko J Leskinen, Aimo Tiihonen
Synthetic schlieren imaging, also known as background oriented schlieren imaging, is used to determine the acoustical field of a focused ultrasound transducer operating at 1.01 MHz frequency with peak pressure amplitude of 0.97 MPa. The measurement setup is composed of a commercial off-the-shelf digital single-lens reflex (DSLR) camera with an ordinary objective, a high power light-emitting diode driven in pulsating mode, water tank, ultrasound transducer, rotation stage, and driving electronics. Measurements are performed in tomographic fashion by rotating the ultrasound transducer within the water tank and photographing an imaged target behind the ultrasound field...
June 2017: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/28678582/efficacy-of-monopolar-dielectric-transmission-radiofrequency-in-panniculus-adiposus-and-cellulite-reduction
#16
Manuel Albornoz-Cabello, Alfonso Javier Ibáñez Vera, Blanca De la Cruz-Torres
INTRODUCTION: Despite its high incidence rate, there are few studies regarding cellulite treatment. Most of them present non-validated evaluation tools. Radio frequency is a focused treatment very used in aesthetics to reduce it. OBJECTIVES: To know the efficacy of Monopolar Dielectric Radiofrequency (MDR) treatment in dynamic application to reduce cellulite, panniculus adiposus and gluteal and posterior thigh regions. METHODS: Experimental study consisting of inferior members of nine women...
July 5, 2017: Journal of Cosmetic and Laser Therapy: Official Publication of the European Society for Laser Dermatology
https://www.readbyqxmd.com/read/28676883/dermoscopy-confocal-microscopy-and-other-non-invasive-tools-for-the-diagnosis-of-non-melanoma-skin-cancers-and-other-skin-conditions
#17
Josep Malvehy, Giovanni Pellacani
Non-melanoma skin cancer (NMSC), including basal cell and squamous cell carcinomas, is the most common form of neoplasm. The incidence of NMSC is increasing worldwide. The cure rate is high with early detection and appropriate treatment. Visual examination is the first screening tool used by clinicians, but it is sometimes not sufficient to discriminate benign from malignant lesions. Skin biopsy with histopathology is the gold standard for differential diagnosis; however, this procedure is invasive and occasionally painful...
July 5, 2017: Acta Dermato-venereologica
https://www.readbyqxmd.com/read/28675987/material-properties-of-human-ocular-tissue-at-7-%C3%A2%C2%B5m-resolution
#18
Daniel Rohrbach, Kazuyo Ito, Harriet O Lloyd, Ronald H Silverman, Kenji Yoshida, Tadashi Yamaguchi, Jonathan Mamou
Quantitative assessment of the material properties of ocular tissues can provide valuable information for investigating several ophthalmic diseases. Quantitative acoustic microscopy (QAM) offers a means of obtaining such information, but few QAM investigations have been conducted on human ocular tissue. We imaged the optic nerve (ON) and iridocorneal angle in 12-µm deparaffinized sections of the human eye using a custom-built acoustic microscope with a 250-MHz transducer (7-µm lateral resolution). The two-dimensional QAM maps of ultrasound attenuation (α), speed of sound ( c), acoustic impedance ( Z), bulk modulus ( K), and mass density (ρ) were generated...
July 1, 2017: Ultrasonic Imaging
https://www.readbyqxmd.com/read/28674501/the-digestive-tract-of-cephalopods-toward-non-invasive-in-vivo-monitoring-of-its-physiology
#19
Giovanna Ponte, Antonio V Sykes, Gavan M Cooke, Eduardo Almansa, Paul L R Andrews
Ensuring the health and welfare of animals in research is paramount, and the normal functioning of the digestive tract is essential for both. Here we critically assess non- or minimally-invasive techniques which may be used to assess a cephalopod's digestive tract functionality to inform health monitoring. We focus on: (i) predatory response as an indication of appetitive drive; (ii) body weight assessment and interpretation of deviations (e.g., digestive gland weight loss is disproportionate to body weight loss in starvation); (iii) oro-anal transit time requiring novel, standardized techniques to facilitate comparative studies of species and diets; (iv) defecation frequency and analysis of fecal color (diet dependent) and composition (parasites, biomarkers, and cytology); (v) digestive tract endoscopy, but passage of the esophagus through the brain is a technical challenge; (vi) high resolution ultrasound that offers the possibility of imaging the morphology of the digestive tract (e...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28672279/%C3%AE-elemene-regulates-endoplasmic-reticulum-stress-to-induce-the-apoptosis-of-nsclc-cells-through-perk-ire1%C3%AE-atf6-pathway
#20
Ying Liu, Zi-Yu Jiang, Yuan-Li Zhou, Hui-Hui Qiu, Gang Wang, Yi Luo, Jing-Bing Liu, Xiong-Wei Liu, Wei-Quan Bu, Jie Song, Li Cui, Xiao-Bin Jia, Liang Feng
Endoplasmic reticulum stress (ERs) has been regarded as an important cause for the pathogenesis of non-small-cell lung cancer (NSCLC). β-elemene is an active component in the essential oil extracted from a medicinal herb, Curcuma wenyujin, and has been reported to be effective against non-small-cell lung cancer (NSCLC). However, the potential effect and underlying mechanisms of β-elemene on regulating ERs to inhibit NSCLC are still unclear. In the present study, A549 cells and Lewis tumor-bearing C57BL/6J mice were established to evaluate this effect...
June 30, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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