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https://www.readbyqxmd.com/read/28321424/cell-based-meniscus-repair-and-regeneration-at-the-brink-of-clinical-translation-a-systematic-review-of-preclinical-studies
#1
Jasmijn V Korpershoek, Tommy S de Windt, Michella H Hagmeijer, Lucienne A Vonk, Daniel B F Saris
BACKGROUND: Meniscus damage can be caused by trauma or degeneration and is therefore common among patients of all ages. Repair or regeneration of the menisci could be of great importance not only for pain relief or regaining function but also to prevent degenerative disease and osteoarthritis. Current treatment does not offer consistent long-term improvement. Although preclinical research focusing on augmentation of meniscal tear repair and regeneration after meniscectomy is encouraging, clinical translation remains difficult...
February 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28278383/3d-printed-poly-%C3%AE%C2%B5-caprolactone-scaffold-augmented-with-mesenchymal-stem-cells-for-total-meniscal-substitution
#2
Zheng-Zheng Zhang, Shao-Jie Wang, Ji-Ying Zhang, Wen-Bo Jiang, Ai-Bing Huang, Yan-Song Qi, Jian-Xun Ding, Xue-Si Chen, Dong Jiang, Jia-Kuo Yu
BACKGROUND: Total meniscectomy leads to knee osteoarthritis in the long term. The poly(ε-caprolactone) (PCL) scaffold is a promising material for meniscal tissue regeneration, but cell-free scaffolds result in relatively poor tissue regeneration and lead to joint degeneration. HYPOTHESIS: A novel, 3-dimensional (3D)-printed PCL scaffold augmented with mesenchymal stem cells (MSCs) would offer benefits in meniscal regeneration and cartilage protection. STUDY DESIGN: Controlled laboratory study...
March 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28219952/enthesis-regeneration-a-role-for-gli1-progenitor-cells
#3
Andrea G Schwartz, Leesa M Galatz, Stavros Thomopoulos
The tendon enthesis originates from a specific pool of hedgehog-active Gli1+ progenitor cells that differentiate and produce mineralized fibrocartilage. The current study investigated the regenerative capacity of this cell population by comparing the responses of early postnatal and mature entheses to injury. Lineage tracing studies demonstrated that the original Gli1+ cell population had the capacity to heal immature entheses after injury but this capacity was lost after the cells differentiated into mature fibrochondrocytes...
February 20, 2017: Development
https://www.readbyqxmd.com/read/28214835/up-regulation-of-tgf-%C3%AE-promotes-tendon-to-bone-healing-after-anterior-cruciate-ligament-reconstruction-using-bone-marrow-derived-mesenchymal-stem-cells-through-the-tgf-%C3%AE-mapk-signaling-pathway-in-a-new-zealand-white-rabbit-model
#4
Rui Wang, Bin Xu, Hong-Gang Xu
BACKGROUND/AIMS: This study aimed to explore the role of TGF-β in tendon-to-bone healing after anterior cruciate ligament (ACL) reconstruction using bone marrow-derived mesenchymal stem cells (BMSCs) through the TGF-β/MAPK signaling pathway in a New Zealand white rabbit model. METHODS: A total of 72 healthy male New Zealand white rabbits were selected for these experiments. Flow cytometry and immunofluorescence were used to detect the expression of BMSC surface markers, and qRT-PCR was performed to detect TGF-β mRNA expression...
January 20, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28161573/chondrogenically-primed-tonsil-derived-mesenchymal-stem-cells-encapsulated-in-riboflavin-induced-photocrosslinking-collagen-hyaluronic-acid-hydrogel-for-meniscus-tissue-repairs
#5
Rachel H Koh, Yinji Jin, Byung-Jae Kang, Nathaniel S Hwang
Current meniscus tissue repairing strategies involve partial or total meniscectomy, followed by allograft transplantation or synthetic material implantation. However, allografts and synthetic implants have major drawbacks such as the limited supply of grafts and lack of integration into host tissue, respectively. In this study, we investigated the effects of conditioned medium (CM) from meniscal fibrochondrocytes and TGF-β3 on tonsil-derived mesenchymal stem cells (T-MSCs) for meniscus tissue engineering. CM-expanded T-MSCs were encapsulated in riboflavin-induced photocrosslinked collagen-hyaluronic acid (COL-RF-HA) hydrogels and cultured in chondrogenic medium containing TGF-β3...
February 1, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/27994624/regulatory-mechanisms-of-the-ihh-pthrp-signaling-pathway-in-fibrochondrocytes-in-entheses-of-pig-achilles-tendon
#6
Xuesong Han, Yanfeng Zhuang, Zhihong Zhang, Lin Guo, Wanming Wang
This study is aimed at exploring the effect of stress stimulation on the proliferation and differentiation of fibrochondrocytes in entheses mediated via the Indian hedgehog (Ihh)/parathyroid hormone-related protein (PTHrP) signaling pathway. Differential stress stimulation on fibrochondrocytes in entheses was imposed. Gene expression and protein levels of signaling molecules including collagen type I (Col I), Col II, Col X, Ihh, and PTHrP in the cytoplasm of fibrochondrocytes were detected. Ihh signal blocking group was set up using Ihh signaling pathway-specific blocking agent cyclopamine...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27989921/a-model-system-for-developing-a-tissue-engineered-meniscal-enthesis
#7
Mary Clare McCorry, Melissa M Mansfield, Xiaozhou Sha, Daniel J Coppola, Jonathan W Lee, Lawrence J Bonassar
The meniscus acts as a stabilizer, lubricator, and load distributer in the knee joint. The mechanical stability of the meniscus depends on its connection to the underlying bone by a fibrocartilage to bone transition zone called the meniscal enthesis. Tissue engineered menisci hold great promise as a treatment alternative however lack a means of integrated fixation to the underlying bone needed in order for a tissue engineered meniscal replacement to be successful. Tissue engineering the meniscal enthesis is a difficult task given the complex gradients of cell type, mineral, and extracellular matrix molecules...
October 29, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27925474/fiber-development-and-matrix-production-in-tissue-engineered-menisci-using-bovine-mesenchymal-stem-cells-and-fibrochondrocytes
#8
Mary Clare McCorry, Lawrence J Bonassar
Mesenchymal stem cells (MSCs) have been investigated with promising results for meniscus healing and tissue engineering. While MSCs are known to contribute to extracellular matrix (ECM) production, less is known about how MSCs produce and align large organized fibers for application to tissue engineering the meniscus. The goal of this study was to investigate the capability of MSCs to produce and organize ECM molecules compared to meniscal fibrochondrocytes (FCCs). Bovine FCCs and MSCs were encapsulated in an anatomically accurate collagen meniscus using monoculture and co-culture of each cell type...
December 7, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27824291/development-of-a-micronized-meniscus-extracellular-matrix-scaffold-for-potential-augmentation-of-meniscal-repair-and-regeneration
#9
Farrah A Monibi, Chantelle C Bozynski, Keiichi Kuroki, Aaron M Stoker, Ferris M Pfeiffer, Seth L Sherman, James L Cook
Decellularized scaffolds composed of extracellular matrix (ECM) hold promise for repair and regeneration of the meniscus, given the potential for ECM-based biomaterials to aid in stem cell recruitment, infiltration, and differentiation. The objectives of this study were to decellularize canine menisci to fabricate a micronized, ECM-derived scaffold and to determine the cytocompatibility and repair potential of the scaffold ex vivo. Menisci were decellularized with a combination of physical agitation and chemical treatments...
December 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27718449/amecm-dcb-scaffold-prompts-successful-total-meniscus-reconstruction-in-a-rabbit-total-meniscectomy-model
#10
Zhiguo Yuan, Shuyun Liu, Chunxiang Hao, Weimin Guo, Shuang Gao, Mingjie Wang, Mingxue Chen, Zhen Sun, Yichi Xu, Yu Wang, Jiang Peng, Mei Yuan, Quan-Yi Guo
Tissue-engineered meniscus regeneration is a very promising treatment strategy for meniscus lesions. However, generating the scaffold presents a huge challenge for meniscus engineering as this has to meet particular biomechanical and biocompatibility requirements. In this study, we utilized acellular meniscus extracellular matrix (AMECM) and demineralized cancellous bone (DCB) to construct three different types of three-dimensional porous meniscus scaffold: AMECM, DCB, and AMECM/DCB, respectively. We tested the scaffolds' physicochemical characteristics and observed their interactions with meniscus fibrochondrocytes to evaluate their cytocompatibility...
December 2016: Biomaterials
https://www.readbyqxmd.com/read/27536780/redifferentiation-of-high-throughput-generated-fibrochondrocyte-micro-aggregates-impact-of-low-oxygen-tension
#11
Elke Berneel, Charlot Philips, Heidi Declercq, Ria Cornelissen
In meniscus tissue engineering strategies, enhancing the matrix quality of the neomeniscal tissue is important. When the differentiated phenotype of fibrochondrocytes is lost, the quality of the matrix becomes compromised. The objective of this study was to produce uniform fibrochondrocyte micro-aggregates with desirable phenotype and tissue homogeneity in large quantities using a simple and reproducible method. Furthermore, we investigated if hypoxia could enhance the matrix quality. Porcine fibrochondrocytes were expanded at 21% oxygen until passage 3 (P3) and a gene expression profile was determined...
2016: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/27347022/osteoarthritis-prevention-and-meniscus-regeneration-induced-by-transplantation-of-mesenchymal-stem-cell-sheet-in-a-rat-meniscal-defect-model
#12
Yiying Qi, Guangnan Chen, Gang Feng
Transplantation of mesenchymal stem cells (MSCs) is a potential therapy for meniscus regeneration. However, when using single cell suspension injection, there is frequently a significant loss of cells, with only a small percentage of cells remaining at the target site. This issue may be solved with the use of MSC sheets. In the present study, we investigated whether the use of MSC sheets were able to regenerate the meniscus effectively in a rat meniscectomized model. The anterior half of the medial meniscus in 10 rats was excised and an MSC sheet was transplanted in the MSC sheet treatment group, while untreated rats served as the control...
July 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27314395/fibrochondrocyte-growth-and-functionality-on-tio%C3%A2-nanothin-films
#13
Sharon Ronald, David K Mills
Disorders affecting the temporomandibular joint (TMJ) are a long-standing health concern. TMJ disorders (TMJD) are often associated with an internal disc derangement accompanied by a suite of symptoms including joint noises, jaw dysfunction, and severe pain. The severity of patient symptoms and their reoccurrence can be alleviated to some extent with conservative therapy; however, refractory cases often require surgery that has shown only limited success. Bioengineered scaffolds with cell supportive surfaces an d nanoarchitectures that mimic TMJ tissue structure may offer an alternative treatment modality...
2016: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/27302206/biomedical-grade-high-mannuronic-acid-content-biomvm-alginate-enhances-the-proteoglycan-production-of-primary-human-meniscal-fibrochondrocytes-in-a-3-d-microenvironment
#14
Ana Rey-Rico, Angelique Klich, Magali Cucchiarini, Henning Madry
Alginates are important hydrogels for meniscus tissue engineering as they support the meniscal fibrochondrocyte phenotype and proteoglycan production, the extracellular matrix (ECM) component chiefly responsible for its viscoelastic properties. Here, we systematically evaluated four biomedical- and two nonbiomedical-grade alginates for their capacity to provide the best three-dimensional (3-D) microenvironment and to support proteoglycan synthesis of encapsulated human meniscal fibrochondrocytes in vitro. Biomedical-grade, high mannuronic acid alginate spheres (BioLVM, BioMVM) were the most uniform in size, indicating an effect of the purity of alginate on the shape of the spheres...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27159292/local-administration-of-simvastatin-stimulates-healing-of-an-avascular-meniscus-in-a-rabbit-model-of-a-meniscal-defect
#15
Shurong Zhang, Takehiko Matsushita, Ryosuke Kuroda, Kyohei Nishida, Tokio Matsuzaki, Tomoyuki Matsumoto, Koji Takayama, Kanto Nagai, Shinya Oka, Yasuhiko Tabata, Kouki Nagamune, Masahiro Kurosaka
BACKGROUND: Repair of an avascular meniscus is challenging because of its low capacity for healing. Several reports have shown that simvastatin stimulates the anabolic activity of intervertebral fibrochondrocytes, suggesting that simvastatin may be used for the treatment of meniscal defects. PURPOSE: To test whether the local administration of simvastatin stimulates healing of an avascular meniscus in rabbits. STUDY DESIGN: Controlled laboratory study...
July 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27113646/rock-inhibition-stimulates-sox9-smad3-dependent-col2a1-expression-in-inner-meniscus-cells
#16
Takayuki Furumatsu, Ami Maehara, Toshifumi Ozaki
BACKGROUND: Proper functioning of the meniscus depends on the composition and organization of its fibrocartilaginous extracellular matrix. We previously demonstrated that the avascular inner meniscus has a more chondrocytic phenotype compared with the outer meniscus. Inhibition of the Rho family GTPase ROCK, the major regulator of the actin cytoskeleton, stimulates the chondrogenic transcription factor Sry-type HMG box (SOX) 9-dependent α1(II) collagen (COL2A1) expression in inner meniscus cells...
July 2016: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
https://www.readbyqxmd.com/read/26971202/characterization-of-mesenchymal-stem-cells-and-fibrochondrocytes-in-three-dimensional-co-culture-analysis-of-cell-shape-matrix-production-and-mechanical-performance
#17
Mary Clare McCorry, Jennifer L Puetzer, Lawrence J Bonassar
BACKGROUND: Bone marrow mesenchymal stem cells (MSCs) have shown positive therapeutic effects for meniscus regeneration and repair. Preliminary in vitro work has indicated positive results for MSC applications for meniscus tissue engineering; however, more information is needed on how to direct MSC behavior. The objective of this study was to examine the effect of MSC co-culture with primary meniscal fibrochondrocytes (FCCs) in a three-dimensional collagen scaffold in fibrochondrogenic media...
March 12, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/26893631/targeted-transplantation-of-iron-oxide-labeled-adipose-derived-mesenchymal-stem-cells-in-promoting-meniscus-regeneration-following-a-rabbit-massive-meniscal-defect
#18
Yiying Qi, Zhigao Yang, Qianhai Ding, Tengfei Zhao, Zhongming Huang, Gang Feng
Repair of a massive meniscal defect remains a challenge in the clinic. However, targeted magnetic cell delivery, an emerging technique, may be useful in its treatment. The present study aimed to determine the effect of targeted intra-articular injection of superparamagnetic iron oxide (SPIO)-labeled adipose-derived mesenchymal stem cells (ASCs) in a rabbit model of a massive meniscal defect. ASCs may be directly labeled and almost 100% of the ASCs were labeled with SPIO after 24 h; these SPIO-labeled ASCs may be orientated by magnet...
February 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/26874799/effect-of-human-serum-and-2-different-types-of-platelet-concentrates-on-human-meniscus-cell-migration-proliferation-and-matrix-formation
#19
Undine Freymann, Sebastian Metzlaff, Jan-Philipp Krüger, Glen Hirsh, Michaela Endres, Wolf Petersen, Christian Kaps
PURPOSE: To evaluate the effect of 10% human serum (HS), 5% platelet-rich plasma (PRP), and 5% autologous conditioned plasma (ACP) on migration, proliferation, and extracellular matrix (ECM) synthesis of human meniscus cells. METHODS: Cell migration and proliferation on stimulation with HS, PRP, and ACP were assessed by chemotaxis assays and measurement of genomic DNA content. Meniscus cells were cultivated in pellets stimulated with 10% HS, 5% PRP, or 5% ACP. Meniscal ECM formation was evaluated by histochemical staining of collagen type I, type II, and proteoglycans and by analysis of fibrochondrocyte marker gene expression...
June 2016: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/26838935/acetabular-labral-reconstruction-with-autologous-tendon-tissue-in-a-porcine-model-in-vivo-histological-assessment-and-gene-expression-analysis-of-the-healing-tissue
#20
Yuan-Yuan Shi, Lin-Xin Chen, Yan Xu, Xiao-Qing Hu, Ying-Fang Ao, Jian-Quan Wang
BACKGROUND: Acetabular labral reconstruction with autologous tendons is the preferred method for treating a severely damaged labrum. However, the healing process of implants remains unknown. Similar to the human acetabular labrum, the porcine acetabular labrum is a fibrocartilage-like tissue. PURPOSE: This study aimed to characterize the histological healing process and gene expression profile of implants in a porcine model of acetabular labral reconstruction. STUDY DESIGN: Descriptive laboratory study...
April 2016: American Journal of Sports Medicine
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