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https://www.readbyqxmd.com/read/28648041/-effects-of-hypoxia-on-the-phenotype-transformation-of-human-dermal-fibroblasts-to-myofibroblasts-and-the-mechanism
#1
B Zhao, F Han, W Zhang, X J Wang, J Zhang, F F Yang, J H Shi, L L Su, D H Hu
Objective: To investigate the effects of hypoxia on the phenotype transformation of human dermal fibroblasts to myofibroblasts and the mechanism. Methods: The third passage of healthy adult human dermal fibroblasts in logarithmic phase were cultured in DMEM medium containing 10% fetal bovine serum for the following five experiments. (1) In experiments 1, 2, and 3, cells were divided into normoxia group and hypoxia group according to the random number table, with 10 dishes in each group. Cells of normoxia group were cultured in incubator containing 21% oxygen, while those of hypoxia group with 1% oxygen...
June 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28648037/-effects-of-activating-silent-information-regulator-1-on-early-kidney-damage-in-rats-with-severe-burn
#2
X Z Bai, T He, Y Liu, W Zhang, F Han, C Yang, W X Cai, Y H Jia, J H Shi, J T Han, L L Su, D H Hu
Objective: To investigate the effects of activating silent information regulator 1 (SIRT1) on the early kidney damage in rats with severe burn. Methods: Thirty healthy male SD rats were divided into sham injury group (SI), pure burn group (PB), and SIRT1 activator group (SA) according to the random number table, with 10 rats in each group. Rats in groups PB and SA were inflicted with 30% total body surface area full-thickness scald (hereinafter referred to as burn) on the back. Immediately after injury, rats in group PB were intraperitoneally injected with normal saline in the dosage of 50 mL/kg, and those in group SA with 1 mg/mL (final mass concentration) resveratrol in the dosage of 50 mL/kg...
June 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28648027/-sirt1-signaling-pathway-mediated-the-protective-effects-on-myocardium-of-rats-after-endurance-training-and-acute-exhaustive-exercise
#3
X Y Li, X Han, H M Zhang, H Tan, S F Han
Objective: To detect the expression of SIRT1 and Ac-FOXO1 in rats after endurance training and acute exhaustive exercise, and explicit the myocardial protective effect of SIRT1. Methods: Rats were randomly divided into four groups: control group(n=20), exhaustive exercise group (E group, n=20), exhaustive exercise group + endurance training (TE group, n=18), exhaustive exercise group + endurance training + selective SIRT1 inhibitor (TSE group, n=17). The Control and E groups were fed routinely for 5 weeks. The TE and TSE groups were subjected to swimming exercise for 5 weeks for endurance exercising...
June 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/28648016/-mechanisms-of-cytoskeleton-and-pi3k%C3%AE-rhoa-in-fine-particulate-matter-deteriorating-phagocytosis-defect-of-alveolar-macrophage-in-mice-with-chronic-obstructive-pulmonary-disease
#4
Q Xia, S T Hu, X L Zeng, H R Bao, X J Liu
Objective: To explore the mechanism of cytoskeleton and PI3Kδ-RhoA in fine particulate matter deteriorating phagocytosis defect of alveolar macrophage (AM) in chronic obstructive pulmonary disease (COPD) mice. Methods: Forty mice were randomly divided into four groups: health control group, COPD group, health PM2.5 group, COPD PM2.5 group and with ten in each group. A mouse model of COPD was established by cigarette smoke exposure, and health PM2.5 group and COPD PM2.5 group mice were given PM2.5 (588 μg/m(3)) aerosol inhalation for 90 days...
June 27, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28648004/-effects-of-botulinum-toxin-type-a-injection-for-pathological-characteristic-of-calf-in-rats-with-spinal-cord-injure
#5
X M Wei, Z L Dou, Y W Zhang, M Dai, F Yu, Q Y Wang, L Jiang
Objective: To explore the effect of BoTN-A on pathological characteristics of calf (GM) and motor function of ankle dorsiflexors in SCI model rats. Methods: Thirty-six SD male rats (weight 260-280 g) were randomly allocated into normal control group and 12w-control group, NS-injection group and BT-injection group in this study.NS/BT-injection groups were divided into two subgroups separately (2w-NS, 2w-BT, 8w-NS, 8w-BT). No injection was applied in normal control group or 12w-control group.Saline/BoTN-A was injected in right GM in NS/BT-injection group at two different time points(2w, 8w)...
June 20, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28647987/-distribution-of-hiv-1-subtypes-among-foreign-patients-in-guangzhou-between-2008-and-2010-and-in-2015
#6
Z G Han, H Wu, C Y Liang, K Gao, H X Mai, Y S Cai, H F Xu
Objective: To understand the characteristic of subtype distribution among foreigners who were living with HIV-1, in Guangzhou. Methods: HIV-1 RNAs were extracted from 114 serum specimens in foreigners diagnosed with HIV-1 infections between 2008 and 2010, and in 2015. Partial pol gene of HIV-1 genome from these RNA samples were amplified by nested reverse transcription polymerase chain reaction (nested-PCR) with nucleotide sequenced. Subsequently, phylogenetic tree was reconstructed using the pol sequences of samples and references...
June 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28647881/alterations-in-soil-microbial-communities-caused-by-treatments-with-penicillin-or-neomycin
#7
Qichun Zhang, Ouyang Kang, Salma Jabeen, Warren A Dick
Antibiotic residues in soils can lead to serious health risk and ecological hazards. In this study, the effects of penicillin and neomycin, two antibiotics widely used in animal production, were investigated on soil bacterial communities. Changes in the community structure were monitored using three 16S ribosomal DNA (rDNA) polymerase chain reaction-based approaches, including denaturing gradient gel electrophoresis (DGGE), amplified rDNA restriction analysis (ARDRA), and terminal-restriction fragment length polymorphism (T-RFLP) analysis...
June 25, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28647863/evaluation-of-cortical-mandibular-bone-in-patients-with-oral-squamous-cell-carcinoma
#8
Gustavo Davi Rabelo, Claudia Coutinho-Camillo, Luiz Paulo Kowalski, Nathalie Portero-Muzy, Jean-Paul Roux, Pascale Chavassieux, Fabio Abreu Alves
OBJECTIVES: The aim of the study was to evaluate the mandible cortical bone changes in patients with oral squamous cell carcinoma (OSCC). PATIENTS AND METHODS: Twenty patients who underwent some mandibular bone removal as part of the treatment of OSCC had bone samples collected in two parts: in the proximity of the tumor (BPT) and in the surgical margin (BEP). Cortical microarchitecture was analyzed trough micro-computed tomography, together with texture analysis, followed by microcrack evaluation in histological sections and gene expression of RANK, RANKL, OPG, and sclerostin by quantitative polymerase chain reaction...
June 24, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28647859/high-touch-surfaces-microbial-neighbours-at-hand
#9
REVIEW
L Cobrado, A Silva-Dias, M M Azevedo, A G Rodrigues
Despite considerable efforts, healthcare-associated infections (HAIs) continue to be globally responsible for serious morbidity, increased costs and prolonged length of stay. Among potentially preventable sources of microbial pathogens causing HAIs, patient care items and environmental surfaces frequently touched play an important role in the chain of transmission. Microorganisms contaminating such high-touch surfaces include Gram-positive and Gram-negative bacteria, viruses, yeasts and parasites, with improved cleaning and disinfection effectively decreasing the rate of HAIs...
June 25, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28647731/recovery-of-vocal-fold-epithelium-after-acute-phonotrauma
#10
Bernard Rousseau, Tsuyoshi Kojima, Carolyn K Novaleski, Emily E Kimball, Carla V Valenzuela, Masanobu Mizuta, James J Daniero, C Gaelyn Garrett, M Preeti Sivasankar
We investigated the timeline of tissue repair of vocal fold epithelium after acute vibration exposure using an in vivo rabbit model. Sixty-five New Zealand white breeder rabbits were randomized to 120 min of modal- or raised-intensity phonation. After the larynges were harvested at 0, 4, 8, and 24 h, and at 3 and 7 days, the vocal fold tissue was evaluated using electron microscopy and quantitative real-time polymerase chain reaction. There was an immediate decrease in the microprojection depth and height following raised-intensity phonation, paired with upregulation of cyclooxygenase-2...
June 24, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/28647730/qiliqiangxin-attenuates-adverse-cardiac-remodeling-after-myocardial-infarction-in-ovariectomized-mice-via-activation-of-ppar%C3%AE
#11
Shutong Shen, Huimin Jiang, Yihua Bei, Jialiang Zhang, Haifeng Zhang, Hongsheng Zhu, Chenlin Zhang, Wenming Yao, Cong Wei, Hongcai Shang, Xinli Li
BACKGROUND/AIMS: This study was designed to investigate the therapeutic effect of traditional Chinese medication Qiliqiangxin (QLQX) on adverse cardiac remodeling after myocardial infarction (MI) in bilateral ovariectomized (OVX) female mice. METHODS: Eight-week old female C57BL/6 mice were operated to ligate the left anterior descending coronary artery seven days after bilateral ovariectomy and were orally administered either QLQX or vehicle. 21 days after ligation, echocardiography was performed to evaluate the heart function of all mice...
June 23, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28647702/comparative-mirna-expression-profile-analysis-of-squamous-cell-carcinoma-and-peritumoral-mucosa-from-the-meso-and-hypopharynx
#12
Eva Orosz, Katalin Gombos, Tamas Riedling, Priscilla Afiakurue, Istvan Kiss, Jozsef Pytel, Imre Gerlinger, Istvan Szanyi
BACKGROUND/AIM: The role of microRNAs (miRNA) in carcinogenesis is related to their genome-regulatory function. The aim of the present study was to identify and compare miRNA expression signatures of meso- and hypopharynx squamous cell cancers in consideration of the cancer field hypothesis. PATIENTS AND METHODS: Thirteen snap-frozen biopsy series of tumors and peritumoral tissues from the meso- and hypopharynx were analyzed regarding their miRNA expression with quantitative real-time polymerase chain reaction (qRT-PCR)...
July 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28647698/increased-expression-of-follistatin-in-breast-cancer-reduces-invasiveness-and-clinically-correlates-with-better-survival
#13
Catherine Zabkiewicz, Jeyna Resaul, Rachel Hargest, Wen Guo Jiang, Lin Ye
BACKGROUND/AIM: Activin and its antagonist follistatin (FST) have been implicated in several solid tumours. This study investigated the role of FST in breast cancer. MATERIALS AND METHODS: FST expression was examined using reverse transcription polymerase chain reaction (RT-PCR), real-time quantitative polymerase chain reaction (qPCR) and immunohistochemistry in a cohort of breast cancer samples. Expression was correlated to pathological and prognostic parameters in our patient cohort...
July 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28647625/thermoresponsive-poly-glycidyl-ether-brushes-on-gold-surface-engineering-parameters-and-their-implication-for-cell-sheet-fabrication
#14
Silke Heinen, José Luis Cuellar-Camacho, Marie Weinhart
Thermoresponsive polymer coatings, optimized for cell adhesion and thermally-triggered cell detachment, allow the fabrication of confluent cell sheets with intact extracellular matrix. However, rational design guidelines for such coatings are rare, since temperature-triggered cell adhesion and detachment from thermoresponsive surfaces are mechanistically not well understood. Herein, we investigated the impact of molecular weight (2, 9, 24 kDa), grafting density (0.04 - 1.4 chains nm(-2)), morphology, and roughness of well-characterized thermoresponsive poly(glycidyl ether) brushes on the cell response at 37 and 20°C...
June 21, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28647624/shape-memory-polyurethanes-with-oxidation-induced-degradation-in-vivo-and-in-vitro-correlations-for-endovascular-material-applications
#15
Andrew C Weems, Kevin T Wacker, James K Carrow, Anthony J Boyle, Duncan J Maitland
The synthesis of thermoset shape memory polymer (SMP) polyurethanes from symmetric, aliphatic alcohols and diisocyanates has previously demonstrated excellent biocompatibility in short term in vitro and in vivo studies, although long term stability has not been investigated. Here we demonstrate that while rapid oxidation occurs in these thermoset SMPs, facilitated by the incorporation of multi-functional, branching amino groups, byproduct analysis does not indicate toxicological concern for these materials...
June 21, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28647583/the-temporal-expression-of-adipokines-during-spinal-fusion
#16
Sohrab Virk, Alicia L Bertone, Hayam Hamaz Hussein, Jeffrey M Toth, Mari Kaido, Safdar Khan
BACKGROUND CONTEXT: Adipokines are secreted by white adipose tissue and have been associated with fracture healing. Our goal was to report the temporal expression of adipokines during spinal fusion in an established rabbit model. PURPOSE: Our goal was to report the temporal expression of adipokines during spinal fusion in an established rabbit model. STUDY DESIGN: Laboratory animal model METHODS: New Zealand white rabbits were assigned to either sham surgery (n=2), unilateral posterior spinal fusion (n=14) or bilateral posterior spinal fusion (n=14)...
June 21, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28647556/synapsin-i-phosphorylation-is-dysregulated-by-beta-amyloid-oligomers-and-restored-by-valproic-acid
#17
Jade Marsh, Saifuddien Haji Bagol, Robin S B Williams, George Dickson, Pavlos Alifragis
Alzheimer's disease is the most prevalent form of dementia in the elderly but the precise causal mechanisms are still not fully understood. Growing evidence supports a significant role for Aβ42 oligomers in the development and progression of Alzheimer's. For example, intracellular soluble Aβ oligomers are thought to contribute to the early synaptic dysfunction associated with Alzheimer's disease, but the molecular mechanisms underlying this effect are still unclear. Here, we identify a novel mechanism that contributes to our understanding of the reported synaptic dysfunction...
June 21, 2017: Neurobiology of Disease
https://www.readbyqxmd.com/read/28647539/chemical-addressability-of-potato-virus-x-for-its-applications-in-bio-nanotechnology
#18
Duc H T Le, He Hu, Ulrich Commandeur, Nicole F Steinmetz
Potato virus X (PVX), a type member of the plant virus potexvirus group, offers a unique nanotechnology platform based on its high aspect ratio, flexible filamentous shape. The PVX platform has already been engineered and studied for its uses in imaging, drug delivery, and immunotherapies. While genetic engineering procedures are well established for PVX, there is limited information about chemical conjugation strategies for functionalizing PVX, partly due to the lack of structural information of PVX at high resolution...
June 21, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28647518/increasing-the-accuracy-of-proteomic-typing-by-decellularisation-of-amyloid-tissue-biopsies
#19
P Patrizia Mangione, Giuseppe Mazza, Janet A Gilbertson, Nigel B Rendell, Diana Canetti, Sofia Giorgetti, Luca Frenguelli, Marco Curti, Tamer Rezk, Sara Raimondi, Mark B Pepys, Philip N Hawkins, Julian D Gillmore, Graham W Taylor, Massimo Pinzani, Vittorio Bellotti
Diagnosis and treatment of systemic amyloidosis depend on accurate identification of the specific amyloid fibril protein forming the tissue deposits. Confirmation of monoclonal immunoglobulin light chain amyloidosis (AL), requiring cytotoxic chemotherapy, and avoidance of such treatment in non-AL amyloidosis, are particularly important. Proteomic analysis characterises amyloid proteins directly. It complements immunohistochemical staining of amyloid to identify fibril proteins and gene sequencing to identify mutations in the fibril precursors...
June 21, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28647469/intra-articular-injection-of-microrna-140-alleviates-osteoarthritis-progression-by-modulating-extracellular-matrix-homeostasis-in-rats
#20
Hai-Bo Si, Yi Zeng, Shu-Yun Liu, Zong-Ke Zhou, You-Nan Chen, Jing-Qiu Cheng, Yan-Rong Lu, Bin Shen
OBJECTIVE: Disruptions of extracellular matrix (ECM) homeostasis are key events in the pathogenesis of osteoarthritis (OA). MicroRNA-140 (miRNA-140) is expressed specifically in cartilage and regulates ECM-degrading enzymes. Our objective in this study was to determine if intra-articular injection of miRNA-140 can attenuate OA progression in rats. DESIGN: miRNA-140 levels in human normal and OA cartilage derived chondrocytes and synovial fluid were assessed by polymerase chain reaction (PCR)...
June 21, 2017: Osteoarthritis and Cartilage
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