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https://www.readbyqxmd.com/read/29679903/isoharringtonine-inhibits-breast-cancer-stem-like-properties-and-stat3-signaling
#1
Wei Chen, Hui Wang, Mei Cheng, Ling Ni, Li Zou, Qin Yang, Xianghai Cai, Baowei Jiao
OBJECTIVES: Breast cancer stem cells (BCSCs) contribute to breast cancer progression, relapse, and treatment resistance. Identification of the natural inhibitory components of BCSCs is therefore critical for clinical treatment. Here, we investigated whether isoharringtonine (IHT) had inhibitory effects on BCSCs in breast cancer cell lines. METHODS: HCC1806, HCC1937, and MCF7 cells were treated with IHT. The proliferation and the migration of cells were detected by MTS assay and wound healing migration assay, respectively...
April 18, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29679893/identification-of-homocysteine-suppressive-mitochondrial-etc-complex-genes-and-tissue-expression-profile-novel-hypothesis-establishment
#2
Ramon Cueto, Lixiao Zhang, Hui Min Shan, Xiao Huang, Xinyuan Li, Ya-Feng Li, Jahaira Lopez, William Y Yang, Muriel Lavallee, Catherine Yu, Yong Ji, Xiaofeng Yang, Hong Wang
Hyperhomocysteinemia (HHcy) is an independent risk factor for cardiovascular disease (CVD) which has been implicated in matochondrial (Mt) function impairment. In this study, we characterized Hcy metabolism in mouse tissues by using LC-ESI-MS/MS analysis, established tissue expression profiles for 84 nuclear-encoded Mt electron transport chain complex (nMt-ETC-Com) genes in 20 human and 19 mouse tissues by database mining, and modeled the effect of HHcy on Mt-ETC function. Hcy levels were high in mouse kidney/lung/spleen/liver (24-14 nmol/g tissue) but low in brain/heart (~5 nmol/g)...
April 4, 2018: Redox Biology
https://www.readbyqxmd.com/read/29679872/maize-productivity-heavy-metals-uptake-and-their-availability-in-contaminated-clay-and-sandy-alkaline-soils-as-affected-by-inorganic-and-organic-amendments
#3
Mahmoud F Seleiman, Ahmed M S Kheir
Contamination of arid and semi-arid ecosystems by toxic heavy metals is a serious concern due to its impact on growth and productivity of crop and health risk through food chain. Therefore, the aims of this investigation were to study the impact of inorganic (i.e. nano hydroxyapatite, NHA and polyacrylamide, PAM) and organic (i.e. sugar beet factory lime, SBFL and biochar, BI) soil amendments on maize productivity grown in contaminated silty clay (i.e. fluvial, lacustrine) and sandy (i.e. marine) alkaline soils...
April 15, 2018: Chemosphere
https://www.readbyqxmd.com/read/29679805/poly-l-lysine-modified-cryogels-for-efficient-bilirubin-removal-from-human-plasma
#4
Mehmet Emin Çorman
In this study, poly-l-lysine (PLL) immobilized PHEMA cryogel was designed for the specific bilirubin removal from human plasma. The surface of PHEMA cryogels is surrounded by PLL chains to enhance specific binding of bilirubin molecules via specific complementary electrostatic interactions. The functionalization of the PHEMA cryogel was carried out by direct coupling of PLL to pre-activated OH-group of the HEMA alcohol units via carbodiimide activation. Prior to removal of bilirubin from human plasma, the optimization parameters including, initial bilirubin concentration, flow rate, temperature, ionic strength, and adsorption rates on adsorption of PLL-PHEMA cryogel were investigated from the aqueous medium...
April 5, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29679794/multi-paratopic-vegf-decoy-receptor-have-superior-anti-tumor-effects-through-anti-egfrs-and-targeted-anti-angiogenic-activities
#5
Dae Hee Lee, Myeong Youl Lee, Youngsuk Seo, Hyo Jeong Hong, Hyun Joo An, Jong Soon Kang, Ho Min Kim
Limitation of current anti-Vascular Endothelial Growth Factor (VEGF) cancer therapy is transitory responses, inevitable relapses and its insufficient tumor-targeting. Thus, multifaceted approaches, including the development of bispecific antibodies and combination strategies targeting different pathways have been proposed as an alternative. Here, we developed a novel multi-paratopic VEGF decoy receptor, Cetuximab-VEGF-Grab and Trastuzumab-VEGF-Grab, by genetically fusing VEGF decoy receptor (VEGF-Grab) to a single chain Fv of anti-Epidermal Growth Factor Receptor (EGFR) antibody (Cetuximab and Trastuzumab)...
April 16, 2018: Biomaterials
https://www.readbyqxmd.com/read/29679786/simultaneous-analysis-of-historical-emerging-and-novel-brominated-flame-retardants-in-food-and-feed-using-a-common-extraction-and-purification-method
#6
Emmanuelle Bichon, Ingrid Guiffard, Anaïs Vénisseau, Elodie Lesquin, Vincent Vaccher, Philippe Marchand, Bruno Le Bizec
Brominated Flame Retardants (BFRs) are still widely used for industrial purposes. These contaminants may enter the food chain where they mainly occur in food of animal origin. The aim of our work was to provide a unique method able to quantify the widest range of BFRs in feed and food items. After freeze-drying and grinding, a pressurized liquid extraction was carried out. The extract was purified on acidified silica, Florisil® and carbon columns, the four separated fractions were analyzed by gas and liquid chromatography coupled to high resolution and tandem mass spectrometry...
April 13, 2018: Chemosphere
https://www.readbyqxmd.com/read/29679756/myh9-structure-functions-and-role-of-non-muscle-myosin-iia-in-human-disease
#7
REVIEW
Alessandro Pecci, Xuefei Ma, Anna Savoia, Robert S Adelstein
The MYH9 gene encodes the heavy chain of non-muscle myosin IIA, a widely expressed cytoplasmic myosin that participates in a variety of processes requiring the generation of intracellular chemomechanical force and translocation of the actin cytoskeleton. Non-muscle myosin IIA functions are regulated by phosphorylation of its 20 kDa light chain, of the heavy chain, and by interactions with other proteins. Variants of MYH9 cause an autosomal-dominant disorder, termed MYH9-related disease, and may be involved in other conditions, such as chronic kidney disease, non-syndromic deafness, and cancer...
April 18, 2018: Gene
https://www.readbyqxmd.com/read/29679724/harvest-and-trade-of-caterpillar-mushroom-ophiocordyceps-sinensis-and-the-implications-for-sustainable-use-in-the-tibet-region-of-southwest-china
#8
Jun He
ETHNOPHARMACOLOGICAL RELEVANCE: caterpillar mushroom (Ophiocordyceps sinensis) is a unique medicinal fungi which is only found in alpine grasslands in Himalayan mountain regions and the Tibetan Plateau. Known locally as Yartsa Gunbu, it has been widely used in Tibetan and Chinese Medicine for centuries. It is crucial to understand local commercial harvest and trade practices of caterpillar mushroom to support the sustainable management of this valuable resource. However, data derived from empirically grounded research is currently limited, particularly in China...
April 18, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29679702/alamandine-attenuates-sepsis-associated-cardiac-dysfunction-via-inhibiting-mapks-signaling-pathways
#9
Peng Li, Xi-Ru Chen, Fei Xu, Chi Liu, Chang Li, Hui Liu, Hui Wang, Wei Sun, Yan-Hui Sheng, Xiang-Qing Kong
Sepsis-induced myocardial dysfunction represents a major cause of death in intensive care units. Alamandine is an important biologically active peptide in the renin-angiotensin system. The present study evaluated whether alamandine improves cardiac dysfunction, inflammation, and apoptosis, and affects the signaling pathways involved in these events. Experiments were carried out in mice treated with lipopolysaccharide (LPS) or alamandine, and in neonatal rat cardiomyocytes. Alamandine increased the ejection fraction and fractional shortening, both of which were decreased upon LPS infusion in mice...
April 18, 2018: Life Sciences
https://www.readbyqxmd.com/read/29679651/use-of-pcr-denaturing-gradient-gel-electrophoresis-for-the-discrimination-of-candida-species-isolated-from-natural-habitats
#10
Abd El-Latif Hesham, Vijai Kumar Gupta, Bhim Pratap Singh
Candida species are opportunistic microbes that cause chronic infections for a human being. Therefore, the exact identification of Candida species is extremely important for improved therapeutic strategy against these species. Identification based on conventional methods cannot differentiate between some of yeasts species, hence PCR based molecular techniques and sequencing could be an alternative tool for the yeasts identification. A quick molecular method based on the polymerase chain reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) was applied for distinguishing strains belonging to the Candida species...
April 18, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29679602/solubility-enhanced-gmyl6-fused-with-a-hexa-lysine-tag-promotes-the-cytotoxicity-of-human-nk-cells
#11
Juanjuan Liu, Yuanyuan Jin, Xiaozhou Feng, Sen Zou, Guangxin Lv, Zhifei Zhang, Zhaoyong Yang
Goat myosin light chain 6 (gMYL6) is a constituent of certain extracted immunization-induced goat anti-cancer bioactive peptides (ACBPs). However, little is known about its activity onto NK cells which are the basic cellular attackers in cancer immunotherapy for patients with malignancies. Because of the complicated extraction process and low yield of gMYL6 out of the goat ACBPs' mixture, the Nano-flow liquid chromatography and C-terminal polycationic tag expression strategy were used to identify and enrich the peptide to investigate its bioactivity against cancers/tumors...
April 18, 2018: Immunology Letters
https://www.readbyqxmd.com/read/29679557/acetaminophen-induced-liver-injury-is-attenuated-in-transgenic-fat-1-mice-endogenously-synthesizing-long-chain-n-3-fatty-acids
#12
Ruibing Feng, Yang Wang, Conghui Liu, Chunyan Yan, Hang Zhang, Huanxing Su, Jing X Kang, Chang-Zhen Shang, Jian-Bo Wan
Acetaminophen (APAP) overdose-caused hepatotoxicity is the most commonly cause of drugs-induced liver failurecharacterized by oxidative stress, mitochondrial dysfunction, and cell damage. Therapeutic efficacy of omega-3 polyunsaturated fatty acids (n-3 PUFAs) in several models of liver disease is well documented. However, the impacts of n-3 PUFA on APAP hepatotoxicity are not adequately addressed. In this study, the fat-1 transgenic mice that synthesize endogenous n-3 PUFA and wild type (WT) littermates were injected intraperitoneally with APAP at the dose of 400 mg/kg to induce liver injury, and euthanized at 0 h, 2 h, 4 h and 6 h post APAP injection for sampling...
April 18, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29679553/potential-diagnostic-role-of-circulating-mirnas-in-breast-cancer-implications-on-clinicopathological-characters
#13
Menha Swellam, Hekmat M El Magdoub, Naglaa M Hassan, Mona M Hefny, Mohamed Emam Sobeih
BACKGROUND: Circulating miRNAs are stable in body fluids and resembles their levels in cancer tissue/cells. They have been expressed in many cancers among them is breast cancer. Authors aimed to investigate the expression level of three circulating oncomiRNAs (miRNA-21, miRNA-222 and miRNA-373) in serum samples as a minimally non-invasive method for early detection of breast cancer, and study their relation with clinicopathological characters. METHODS: MiRNAs expression levels were determined using quantitative real-time polymerase chain reaction (qPCR) in serum samples from three groups: primary breast cancer patients (n = 137), benign breast lesion patients (n = 60), and healthy individuals as control group (n = 38)...
April 18, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29679546/lithium-chloride-licl-induced-autophagy-and-downregulated-expression-of-transforming-growth-factor-%C3%AE-induced-protein-tgfbi-in-granular-corneal-dystrophy
#14
Danyao Nie, Yun Peng, Ming Li, Xinhua Liu, Meilan Zhu, Lin Ye
This study evaluated whether lithium chloride (LiCl) prevents cytoplasmic accumulation of mutant-transforming growth factor β-induced protein (Mut-TGFBI) in granular corneal dystrophy (GCD) via activation of the autophagy pathway. Levels of TGFBI and microtubule-associated protein 1A/1B-light chain 3 (LC3) in 3 GCD patients and healthy controls were analyzed by immunohistochemistry (IHC) staining and Western blot. Primary corneal fibroblasts were isolated and transfected with wild type or mutant type TGFBI over-expressed vectors, and then treated with LiCl and/or autophagy inhibitor 3-methyladenine (3-MA)...
April 18, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29679529/influence-of-human-milk-and-parenteral-lipid-emulsions-on-serum-fatty-acid-profiles-in-extremely-preterm-infants
#15
Anders K Nilsson, Chatarina Löfqvist, Svetlana Najm, Gunnel Hellgren, Karin Sävman, Mats X Andersson, Lois E H Smith, Ann Hellström
BACKGROUND: Infants born prematurely are at risk of a deficiency in ω-6 and ω-3 long-chain polyunsaturated fatty acids (LC-PUFAs) arachidonic acid (AA) and docosahexaenoic acid (DHA). We investigated how fatty acids from breast milk and parenteral lipid emulsions shape serum LC-PUFA profiles in extremely preterm infants during early perinatal life. METHODS: Ninety infants born < 28 weeks gestational age were randomized to receive parenteral lipids with or without the ω-3 LC-PUFAs eicosapentaenoic acid (EPA) and DHA (SMOFlipid: Fresenius Kabi, Uppsala, Sweden, or Clinoleic: Baxter Medical AB, Kista, Sweden, respectively)...
April 21, 2018: JPEN. Journal of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/29679476/linear-ubiquitin-chain-binding-domains
#16
REVIEW
Lilian Fennell, Simin Rahighi, Fumiyo Ikeda
Ubiquitin modification (ubiquitination) of target proteins can vary with respect to chain lengths, linkage type, and chain forms, such as homologous, mixed and branched ubiquitin chains. Thus, ubiquitination can generate multiple unique surfaces on a target protein substrate. Ubiquitin-binding domains (UBDs) recognize ubiquitinated substrates, by specifically binding to these unique surfaces, modulate the formation of cellular signaling complexes and regulate downstream signaling cascades. Among the eight different homotypic chain types, Met1-linked (also termed linear) chains are the only chains in which linkage occurs on a non-Lys residue of ubiquitin...
April 21, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29679463/cdp-choline-protects-liver-from-ischemia-reperfusion-injury-preserving-mitochondrial-function-and-reducing-oxidative-stress
#17
Cecilia Zazueta, Mabel Buelna-Chontal, Arturo Macías-López, Nadia G Román-Anguiano, Héctor González-Pacheco, Natalia Pavón, Rashidi Springall, Alberto Aranda-Frausto, Rafael Bojalil, Alejandro Silva-Palacios, Rodrigo Velázquez-Espejel, Sonia Galvan Arzate, Francisco Correa
CDP-choline (citicoline) participates as intermediary in the synthesis of phosphatidylcholine, an essential component of cellular membranes. Citicoline treatment has shown beneficial effects in cerebral ischemia, but its potential to diminish reperfusion damage in liver has not been explored. In this work, we evaluated the hepatoprotective effect of citicoline and its possible association with inflammatory/oxidative stress and mitochondrial function, as they are main cellular features of reperfusion damage...
April 20, 2018: Liver Transplantation
https://www.readbyqxmd.com/read/29679435/validation-of-host-specific-bacteroidales-quantitative-pcr-assays-and-their-application-to-microbial-source-tracking-of-drinking-water-sources-in-the-kathmandu-valley-nepal
#18
Bikash Malla, Rajani Ghaju Shrestha, Sarmila Tandukar, Dinesh Bhandari, Daisuke Inoue, Kazunari Sei, Yasuhiro Tanaka, Jeevan B Sherchand, Eiji Haramoto
AIMS: To validate host-specific Bacteroidales assays to identify faecal-source contamination of drinking water sources in the Kathmandu Valley, Nepal. METHODS AND RESULTS: A total of 54 composite faecal-source samples were collected from human sewage, ruminants, pigs, dogs, chickens, and ducks, which were analyzed by quantitative polymerase chain reaction using human-specific (BacHum, HF183 SYBR, gyrB, and HF183 TaqMan), ruminant-specific (BacCow and BacR), pig-specific (Pig2Bac and PF163), and dog-specific assays (BacCan SYBR)...
April 20, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29679429/molecular-diagnosis-of-strongyloides-stercoralis-among-transplant-candidates
#19
Fabiana M Paula, Fernanda M Malta, Priscilla D Marques, Gessica B Melo, Marcelo A Corral, Maiara Gottardi, João R R Pinho, Elenice M N Gonçalves, Vera L P Castilho, Ligia C Pierrotti, Edson Abdala, Silvia F Costa, Pedro P Chieffi, Ronaldo C B Gryschek
Strongyloidiasis can occur without any symptoms or as a potentially fatal hyperinfection or disseminated infection, principally in immunosuppressed patients. Our study aimed to evaluate the application of conventional polymerase chain reaction (cPCR) and real-time PCR (qPCR). Polymerase chain reaction (PCR) and real-time PCR (qPCR) targeting the 18S rRNA gene for detection of Strongyloides stercoralis infection among transplant candidates were applied in stool samples obtained from 150 transplant candidates, preliminarily analyzed by parasitological methods...
April 20, 2018: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/29679359/the-impact-of-national-policies-on-animal-disease-reporting-within-selected-pacific-island-countries-and-territories-picts
#20
Andrew Tukana, Robert Hedlefs, Bruce Gummow
A semi-systematic literature review of national policies was carried out in relation to surveillance and disease reporting in Pacific Island Countries and Territories (PICTs). It also analysed the animal disease reporting structures in Fiji, Papua New Guinea (PNG), Vanuatu and the Solomon Islands. The strengths, weaknesses, opportunities and threats (SWOT) of those reporting structures were examined in relation to how they impacted the detection and management of animal diseases in PICTs. Field missions collected information on animal disease reporting structures and these were discussed in detail with country officials and documented...
April 20, 2018: Tropical Animal Health and Production
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