Takuma Tsuchida, Youngmin A Lee, Naoto Fujiwara, Maria Ybanez, Brittany Allen, Sebastiao Martins, M Isabel Fiel, Nicolas Goossens, Hsin-I Chou, Yujin Hoshida, Scott L Friedman
BACKGROUND AND AIMS: Although the majority of patients with nonalcoholic fatty liver disease (NAFLD) have only steatosis without progression, a sizable fraction develop non-alcoholic steatohepatitis (NASH), which can lead to cirrhosis and hepatocellular carcinoma (HCC). Many established diet-induced mouse models for NASH require 24-52 weeks, which makes testing for drug response costly and time consuming. METHODS: We have sought to establish a murine NASH model with rapid progression of extensive fibrosis and HCC by using a western diet (WD), which is high-fat, high-fructose and high-cholesterol, combined with low dose weekly intraperitoneal carbon tetrachloride (CCl4 ), which served as an accelerator...
March 20, 2018: Journal of Hepatology