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https://www.readbyqxmd.com/read/28732144/influence-of-high-latitude-light-conditions-on-sensory-quality-and-contents-of-health-and-sensory-related-compounds-in-swede-roots-brassica-napus-l-ssp-rapifera-metzg
#1
Jørgen A B Mølmann, Sidsel Fiskaa Hagen, Gunnar B Bengtsson, Tor J Johansen
BACKGROUND: Vegetable growers in Arctic areas usually must rely on market strategies based on regional origin and product quality. Swede roots (rutabaga) were grown in a phytotron to investigate the effect of high latitude light conditions on sensory quality and some health and sensory-related compounds. Experimental treatments included modifications of 24 h natural day length (69° 39' N), by moving plants at daily intervals to dark chambers with either no light, fluorescent growth light and/or low intensity photoperiod extension...
July 21, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28731618/controlling-the-near-infrared-transparency-of-costal-cartilage-by-impregnation-with-clearing-agents-and-magnetite-nanoparticles
#2
Yulia Alexandrovskaya, Kirill Sadovnikov, Andrey Sharov, Anna Sherstneva, Evgeniy Evtushenko, Alexander Omelchenko, Mariya Obrezkova, Valery Tuchin, Valery Lunin, Emil Sobol
Penetration depth of near infrared laser radiation to costal cartilage is controlled by the tissue absorption and scattering and it is the critical parameter to provide the relaxation of mechanical stress throughout the whole thickness of cartilage implant. To enhance the penetration for the laser radiation on 1.56 μm the optical clearing solutions of glycerol and fructose of various concentrations are tested. The effective and reversible tissue clearance was achieved. However, the increasing absorption of radiation should be concerned: 5-8(ᴼ) C increase of tissue temperature was detected...
July 21, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28731563/improving-fructose-utilization-and-butanol-production-by-clostridium-acetobutylicum-via-extracellular-redox-potential-regulation-and-intracellular-metabolite-analysis
#3
Li-Jie Chen, You-Duo Wu, Chuang Xue, Feng-Wu Bai
Jerusalem artichoke (JA) can grow well in marginal lands with high biomass yield, and thus is a potential energy crop for biorefinery. The major biomass of JA is from tubers, which contain inulin that can be easily hydrolyzed into a mixture of fructose and glucose, but fructose utilization for producing butanol as an advanced biofuel is poor compared to glucose-based ABE fermentation by Clostridium acetobutylicum. In this article, the impact of extracellular redox potential (ORP) on the process was studied using a mixture of fructose and glucose to simulate the hydrolysate of JA tubers...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28729959/shot-gun-proteome-and-transcriptome-mapping-of-the-jujube-floral-organ-and-identification-of-a-pollen-specific-s-locus-f-box-gene
#4
Ruihong Chen, Guoliang Chen, Jian Huang
The flower is a plant reproductive organ that forms part of the fruit produced as the flowering season ends. While the number and identity of proteins expressed in a jujube (Ziziphus jujuba Mill.) flower is currently unknown, integrative proteomic and transcriptomic analyses provide a systematic strategy of characterizing the floral biology of plants. We conducted a shotgun proteomic analysis on jujube flowers by using a filter-aided sample preparation tryptic digestion, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
2017: PeerJ
https://www.readbyqxmd.com/read/28728011/anti-inflammatory-effect-of-sugar-amino-acid-maillard-reaction-products-on-intestinal-inflammation-model-in%C3%A2-vitro-and-in%C3%A2-vivo
#5
Jun-Gu Oh, Su-Hyun Chun, Da Hyun Kim, Jin Hye Kim, Hye Soo Shin, Yong Soo Cho, Yong Ki Kim, Hee-Don Choi, Kwang-Won Lee
The Maillard reaction is a nonenzymatic reaction between an amino acid and a reducing sugar that usually occurs upon heating. This reaction occurs routinely in cooking, generates numerous products, which are collectively referred to as Maillard reaction products (MRPs) contributing to aroma and color features. Advanced glycation end-products (AGEs) transformed from MRPs are participated in many types of inflammation reaction. In this study, various sugar-amino acid MRPs were prepared from three different amino acids (lysine, arginine, and glycine) and sugars (glucose, fructose, and galactose) for 1 h with heating at 121 °C...
July 11, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28727103/effects-of-dietary-calcium-fructoborate-supplementation-on-joint-comfort-and-flexibility-and-serum-inflammatory-markers-in-dogs-with-osteoarthritis
#6
A K Price, M R C de Godoy, T A Harper, K E Knap, S Joslyn, Z Pietrzkowski, B K Cross, K B Detweiler, K S Swanson
Our objective was to evaluate the short-term effects of calcium fructoborate (CFB) on gait, joint range of motion, serum inflammatory markers, and owner perception of pain in client-owned dogs. We used 59 osteoarthritic dogs with impairment, with dogs being randomly assigned to 4 treatments: placebo (60 mg fructose; = 15), low dose (69 mg CFB; = 14), high dose (127 mg CFB; = 14), or combination (69 mg CFB, 500 mg glucosamine hydrochloride and 200 mg chondroitin sulfate; = 16). Dogs up to 22.9 kg received 1 capsule/d, while dogs weighing 23 to 50 kg received 2 capsules/d...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28727036/supplementation-of-glycerol-or-fructose-via-drinking-water-to-grazing-lambs-on-tissue-glycogen-level-and-lipogenesis
#7
G Volpi-Lagreca, S K Duckett
Lambs ( = 18; 40.1 ± 7.4 kg BW) were used to assess supplementation of glycerol or fructose via drinking water on growth, tissue glycogen levels, postmortem glycolysis, and lipogenesis. Lambs were blocked by BW and allocated to alfalfa paddocks (2 lambs/paddock and 3 paddocks/treatment). Each paddock within a block was assigned randomly to drinking water treatments for 30 d: 1) control (CON), 2) 120 g fructose/L of drinking water (FRU), or 3) 120 g glycerol/L of drinking water (GLY). Lambs grazed alfalfa with free access to water treatments for 28 d and then were fasted in indoor pens for a final 2 d with access to only water treatments...
June 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28726643/reconstructed-ancestral-enzymes-reveal-that-negative-selection-drove-the-evolution-of-substrate-specificity-in-adp-dependent-kinases
#8
Víctor Castro-Fernandez, Alejandra Herrera-Morande, Ricardo Zamora, Felipe Merino, Felipe Gonzalez-Ordenes, Felipe Padilla-Salinas, Humberto M Pereira, Jose Brandão-Neto, Richard C Garratt, Victoria Guixe
One central goal in molecular evolution is to pinpoint the mechanisms and evolutionary forces that cause an enzyme to change its substrate specificity; however, these processes remain largely unexplored. Using the glycolytic ADP-dependent kinases of archaea, including the orders Thermococcales, Methanosarcinales, and Methanococcales, as a model and employing an approach involving paleoenzymology, evolutionary statistics, and protein structural analysis, we could track changes in substrate specificity during ADP-dependent kinase evolution along with the structural determinants of these changes...
July 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28725624/incretin-effect-of-urena-lobata-leaves-extract-on-structure-and-function-of-rats-islet-%C3%AE-cells
#9
Y Purnomo, D W Soeatmadji, S B Sumitro, M A Widodo
This study aims to determine the incretin effects of Urena lobata leaves extract on the structure and function of rats islet β-cells. This study utilizes male Sprague-Dawley rats divided into 2 control group and 3 test group (n = 5). Diabetic rats were induced with High Fructose Diet (HFD) and single dose intraperitoneal streptozotocin 25 mg/kg bw. Aqueous leaves extract of U. lobata was prepared by decoction methods and administrated orally with doses of 250, 500, and 1000 mg/kg bw for 4 weeks then incretin effect was evaluated by measuring serum GLP-1, insulin, and blood glucose levels...
July 2017: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/28725337/complete-genome-sequence-of-leuconostoc-suionicum-dsm-20241-t-provides-insights-into-its-functional-and-metabolic-features
#10
Byung Hee Chun, Se Hee Lee, Hye Hee Jeon, Dong-Woon Kim, Che Ok Jeon
The genome of Leuconostoc suionicum DSM 20241(T) (=ATCC 9135(T) = LMG 8159(T) = NCIMB 6992(T)) was completely sequenced and its fermentative metabolic pathways were reconstructed to investigate the fermentative properties and metabolites of strain DSM 20241(T) during fermentation. The genome of L. suionicum DSM 20241(T) consists of a circular chromosome (2026.8 Kb) and a circular plasmid (21.9 Kb) with 37.58% G + C content, encoding 997 proteins, 12 rRNAs, and 72 tRNAs. Analysis of the metabolic pathways of L...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28724959/hepatic-metabolic-effects-of-curcuma-longa-extract-supplement-in-high-fructose-and-saturated-fat-fed-rats
#11
Fabrice Tranchida, Zo Rakotoniaina, Laetitia Shintu, Léopold Tchiakpe, Valérie Deyris, Mehdi Yemloul, Pierre Stocker, Nicolas Vidal, Odile Rimet, Abel Hiol, Stefano Caldarelli
The metabolic effects of an oral supplementation with a Curcuma longa extract, at a dose nutritionally relevant with common human use, on hepatic metabolism in rats fed a high fructose and saturated fatty acid (HFS) diet was evaluated. High-resolution magic-angle spinning NMR and GC/MS in combination with multivariate analysis have been employed to characterize the NMR metabolite profiles and fatty acid composition of liver tissue respectively. The results showed a clear discrimination between HFS groups and controls involving metabolites such as glucose, glycogen, amino acids, acetate, choline, lysophosphatidylcholine, phosphatidylethanolamine, and β-hydroxybutyrate as well as an increase of MUFAs and a decrease of n-6 and n-3 PUFAs...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724642/fructose-back-to-the-future
#12
John L Sievenpiper
No abstract text is available yet for this article.
July 19, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28724270/synergistic-effects-of-ad-libitum-low-dose-fructose-drinking-and-low-dose-streptozotocin-treatment-in-wistar-rats-a-mild-model-of-type-2-diabetes
#13
Asie Sadeghi, Maani Beigy, Samira Alizadeh, Hossein Mazloom, Sanaz Vakili, Saideh Ahmadi, Reza Meshkani
To develop a convenient animal model of T2D by pretreatment with low-dose 10% w/v fructose (FRC) solution followed by the injection of low doses of streptozotocin (STZ) in Wistar rats. For this 8-week experimental study; rats were first fed a standard chow ad-libitum diet and either tap water (n=40) or 10% w/v FRC solution (n=40) for 4 weeks. Next, rats in each category were randomly allocated to 4 subgroups (n=10 each) of low-dose STZ (25,35, and 45 mg/kg). The final mean fasting blood sugar (FBG) of FRC+STZ45 (197±55...
May 2017: Acta Medica Iranica
https://www.readbyqxmd.com/read/28723898/fructose-1-6-bisphosphate-and-aldolase-mediate-glucose-sensing-by-ampk
#14
Chen-Song Zhang, Simon A Hawley, Yue Zong, Mengqi Li, Zhichao Wang, Alexander Gray, Teng Ma, Jiwen Cui, Jin-Wei Feng, Mingjiang Zhu, Yu-Qing Wu, Terytty Yang Li, Zhiyun Ye, Shu-Yong Lin, Huiyong Yin, Hai-Long Piao, D Grahame Hardie, Sheng-Cai Lin
The major energy source for most cells is glucose, from which ATP is generated via glycolysis and/or oxidative metabolism. Glucose deprivation activates AMP-activated protein kinase (AMPK), but it is unclear whether this activation occurs solely via changes in AMP or ADP, the classical activators of AMPK. Here, we describe an AMP/ADP-independent mechanism that triggers AMPK activation by sensing the absence of fructose-1,6-bisphosphate (FBP), with AMPK being progressively activated as extracellular glucose and intracellular FBP decrease...
July 19, 2017: Nature
https://www.readbyqxmd.com/read/28720943/non-enzymatic-browning-due-to-storage-is-reduced-by-using-clarified-lemon-juice-as-acidifier-in-industrial-scale-production-of-canned-peach-halves
#15
Domingo Saura, Salud Vegara, Nuria Martí, Manuel Valero, José Laencina
Non-enzymatic browning (NEB) in canned peach halves in syrup during storage was investigated. Absorbance at 420 nm (A420), colorimetric parameters (CIELab, TCD and La/b), fructose, glucose and sucrose, total sugar, organic acids, ascorbic acid (AA), dehydroascorbic acid, and 2,3-diketogulonic acid were used to estimate the extent of NEB during 1 year of storage at 30 °C and the relationships between each of these parameters and A420 were established. The investigation was carried out to explore the possibility of replacing the E330 commonly used as acidifier by turbid or clarified lemon juice (TLJ or CLJ) to obtain a product having good nutrition with better retention of quality...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28720574/fructose-1-6-bisphosphatase-inhibits-erk-activation-and-bypasses-gemcitabine-resistance-in-pancreatic-cancer-by-blocking-iqgap1-mapk-interaction
#16
Xin Jin, Yunqian Pan, Liguo Wang, Tao Ma, Lizhi Zhang, Amy H Tang, Daniel D Billadeau, Heshui Wu, Haojie Huang
Dysregulation of the mitogen-activated protein kinase (MAPK) pathway correlates with progression of pancreatic ductal adenocarcinoma (PDAC) progression. IQ motif containing GTPase activating protein 1 (IQGAP1) is a MAPK scaffold that directly regulates the activation of RAF, MEK, and extracellular signaling-regulated kinases (ERK). Fructose-1,6-bisphosphatase (FBP1), a key enzyme in gluconeogenesis, is transcriptionally downregulated in various cancers, including PDAC. Here we demonstrate that FBP1 acts as a negative modulator of the IQGAP1-MAPK signaling axis in PDAC cells...
July 18, 2017: Cancer Research
https://www.readbyqxmd.com/read/28720279/metabolomic-analysis-identifies-altered-metabolic-pathways-in-multiple-sclerosis
#17
Simone Poddighe, Federica Murgia, Lorena Lorefice, Sonia Liggi, Eleonora Cocco, Maria Giovanna Marrosu, Luigi Atzori
Multiple sclerosis (MS) is a chronic, demyelinating disease that affects the central nervous system and is characterized by a complex pathogenesis and difficult management. The identification of new biomarkers would be clinically useful for more accurate diagnoses and disease monitoring. Metabolomics, the identification of small endogenous molecules in, offers an instantaneous molecular snapshot of the MS phenotype. Here the metabolomic profiles (utilizing plasma from patients with MS) were characterized with a Gas cromatography-mass spectrometry-based platform followed by a multivariate statistical analysis and comparison with a healthy control (HC) population...
July 15, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28719195/fluorinated-boronic-acid-appended-pyridinium-salts-and-19f-nmr-spectroscopy-for-diol-sensing
#18
Jörg Axthelm, Sven H C Askes, Martin Elstner, Upendar Reddy Gandra, Helmar Görls, Peter Bellstedt, Alexander Schiller
The identification and discrimination of diols is of fundamental importance in medical diagnostics, such as measuring the contents of glucose in the urine of diabetes patients. Diol sensors are often based on fluorophore-appended boronic acids, but these severely lack discrimination power and their response is one-dimensional. As an alternative strategy, we present the use of fluorinated boronic acid-appended pyridinium salts in combination with 19F NMR spectroscopy. A pool of 59 (bio)analytes was screened, containing monosaccharides, phosphorylated and N-acetylated sugars, polyols, carboxylic acids, nucleotides and amines...
July 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28718839/dose-dependent-prebiotic-effect-of-lactulose-in-a-computer-controlled-in-vitro-model-of-the-human-large-intestine
#19
Melanie K Bothe, Annet J H Maathuis, Susann Bellmann, Jos M B M van der Vossen, Dirk Berressem, Annalena Koehler, Susann Schwejda-Guettes, Barbara Gaigg, Angelika Kuchinka-Koch, John F Stover
Lactulose, a disaccharide of galactose and fructose, used as a laxative or ammonia-lowering drug and as a functional food ingredient, enhances growth of Bifidobacterium and Lactobacillus at clinically relevant dosages. The prebiotic effect of subclinical dosages of Lactulose, however, remains to be elucidated. This study analyses changes in the microbiota and their metabolites after a 5 days Lactulose treatment using the TIM-2 system, a computer-controlled model of the proximal large intestine representing a complex, high density, metabolically active, anaerobic microbiota of human origin...
July 18, 2017: Nutrients
https://www.readbyqxmd.com/read/28715508/biofilm-extracellular-polysaccharides-degradation-during-starvation-and-enamel-demineralization
#20
Bárbara Emanoele Costa Oliveira, Jaime Aparecido Cury, Antônio Pedro Ricomini Filho
This study was conducted to evaluate if extracellular polysaccharides (EPS) are used by Streptococcus mutans (Sm) biofilm during night starvation, contributing to enamel demineralization increasing occurred during daily sugar exposure. Sm biofilms were formed during 5 days on bovine enamel slabs of known surface hardness (SH). The biofilms were exposed to sucrose 10% or glucose + fructose 10.5% (carbohydrates that differ on EPS formation), 8x/day but were maintained in starvation during the night. Biofilm samples were harvested during two moments, on the end of the 4th day and in the morning of the 5th day, conditions of sugar abundance and starvation, respectively...
2017: PloS One
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