Hao Huang, Tian-Tian Tong, Lee-Fong Yau, Jing-Rong Wang, Mao-Hua Lai, Chun-Ren Zhang, Xiao-Hui Wen, Shu-Na Li, Kun-Yin Li, Jian-Qiao Liu, Hong-Xia Ma, Benjamin K Tsang, Zhi-Hong Jiang
Targeted proteomics has advantages over earlier conventional technologies for protein detection. We developed and validated an LC/MRM-MS-based targeted proteomic method combined with immunoaffinity precipitation for the enrichment and detection of low abundance chemerin isoforms in human biofluids. After tryptic digestion, each chemerin isoform was characterized by isoform-specific peptides, and the absolute quantification was achieved by using stable isotope-labeled peptides as internal standards. In serum, follicular fluid and synovial fluid, a total of 6 chemerin isoforms were identified and quantified, among which a novel natural isoform 153Q was discovered for the first time...
December 1, 2020: Analytica Chimica Acta