keyword
MENU ▼
Read by QxMD icon Read
search

Proteomic follicular fluid human

keyword
https://www.readbyqxmd.com/read/27023353/assessment-of-blood-contamination-in-biological-fluids-using-maldi-tof-ms
#1
Katrina Laks, Tiina Kirsipuu, Tuuli Dmitrijeva, Andres Salumets, Peep Palumaa
Biological fluid sample collection often includes the risk of blood contamination that may alter the proteomic profile of biological fluid. In proteomics studies, exclusion of contaminated samples is usually based on visual inspection and counting of red blood cells in the sample; analysis of specific blood derived proteins is less used. To fill the gap, we developed a fast and sensitive method for ascertainment of blood contamination in crude biological fluids, based on specific blood-derived protein, hemoglobin detection by MALDI-TOF MS...
June 2016: Protein Journal
https://www.readbyqxmd.com/read/26098221/from-global-proteome-profiling-to-single-targeted-molecules-of-follicular-fluid-and-oocyte-contribution-to-embryo-development-and-ivf-outcome
#2
REVIEW
Moncef Benkhalifa, Aicha Madkour, Noureddine Louanjli, Nouzha Bouamoud, Brahim Saadani, Ismail Kaarouch, Hikmat Chahine, Omar Sefrioui, Philippe Merviel, Henri Copin
The development of in vitro fertilization (IVF) techniques for infertility management has led to the investigation of the proteome of follicular fluid and oocyte. In addition, different markers contributing to oocyte maturation and embryo development potential have been reported in the literature. Different techniques were utilized to analyze whole proteome or single protein markers in follicular fluid and oocytes, particularly in animal models. Data from several studies have generated large amounts of information, however, an ideal profile to predict the best oocytes and embryos suitable for implantation are still to be uncovered...
August 2015: Expert Review of Proteomics
https://www.readbyqxmd.com/read/26094490/preconception-folic-acid-use-influences-the-follicle-fluid-proteome
#3
John M Twigt, Karel Bezstarosti, Jeroen Demmers, Jan Lindemans, Joop S E Laven, Régine P Steegers-Theunissen
BACKGROUND: The investigation of the human follicle fluid proteome has gained much interest in the search of new markers as predictors for in vitro fertilization and intracytoplasmic sperm injection (IVF/ICSI) treatment outcome. Follicular fluid folate, as substrate of one carbon (1-C) metabolism, affects follicular metabolism and oocyte and embryo quality. From this background, we aim to identify a folate-related follicle fluid proteome that associates with IVF/ICSI treatment outcome...
August 2015: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/25892677/afamin-a-pleiotropic-glycoprotein-involved-in-various-disease-states
#4
REVIEW
Hans Dieplinger, Benjamin Dieplinger
The human glycoprotein afamin was discovered as the fourth member of the albumin gene family. Despite intense research over the last 20 years, our knowledge of afamin's physiological or pathophysiological functions is still very limited. Circulating afamin is primarily of hepatic origin and abundant concentrations are found in plasma, cerebrospinal, ovarian follicular and seminal fluids. In vitro binding studies revealed specific binding properties for vitamin E. A previously performed analytical characterization and clinical evaluation study of an enzyme-linked immunosorbent assay for quantitative measurement of afamin in human plasma demonstrated that the afamin assay meets the quality specifications for laboratory medicine...
June 15, 2015: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/25838815/proteomic-analysis-of-human-follicular-fluid-from-fertile-women
#5
Alberuni M Zamah, Maria E Hassis, Matthew E Albertolle, Katherine E Williams
BACKGROUND: Follicular fluid is a unique biological fluid in which the critical events of oocyte and follicular maturation and somatic cell-germ cell communication occur. Because of the intimate proximity of follicular fluid to the maturing oocyte, this fluid provides a unique window into the processes occurring during follicular maturation. A thorough identification of the specific components within follicular fluid may provide a better understanding of intrafollicular signaling, as well as reveal potential biomarkers of oocyte health for women undergoing assisted reproductive treatment...
2015: Clinical Proteomics
https://www.readbyqxmd.com/read/25595538/preliminary-proteomic-analysis-on-the-alterations-in-follicular-fluid-proteins-from-women-undergoing-natural-cycles-or-controlled-ovarian-hyperstimulation
#6
Yan-Ting Wu, Yan Wu, Jun-Yu Zhang, Ning-Ning Hou, Ai-Xia Liu, Jie-Xue Pan, Jie-Yang Lu, Jian-Zhong Sheng, He-Feng Huang
PURPOSE: To study the differences in protein expression profiles of follicular fluid (FF) between controlled ovarian hyperstimulation (COH) and natural ovulatory cycles. METHODS: Twelve infertile women undergoing in vitro fertilization and embryo transfer (IVF-ET), with matched clinical information, were retrospectively recruited in the IVF center of our university hospital, including six undergoing COH and another six with natural cycles. FF was sampled from dominant follicles with mature oocytes...
March 2015: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/25393639/proteomics-of-follicular-fluid-from-women-with-polycystic-ovary-syndrome-suggests-molecular-defects-in-follicular-development
#7
Aditi S Ambekar, Dhanashree S Kelkar, Sneha M Pinto, Rakesh Sharma, Indira Hinduja, Kusum Zaveri, Akhilesh Pandey, T S Keshava Prasad, Harsha Gowda, Srabani Mukherjee
CONTEXT: Polycystic ovary syndrome (PCOS), a major cause of anovulatory infertility, is characterized by arrested follicular growth. Altered protein levels in the follicular fluid surrounding the ovum may reflect the molecular defects of folliculogenesis in these women. OBJECTIVE: To identify differentially regulated proteins in PCOS by comparing the follicular fluid protein repertoire of PCOS with healthy women. METHODS: The follicular fluid samples were collected from PCOS and normo-ovulatory women undergoing in vitro fertilization...
February 2015: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/25351721/overlap-of-proteomics-biomarkers-between-women-with-pre-eclampsia-and-pcos-a-systematic-review-and-biomarker-database-integration
#8
REVIEW
Gulafshana Hafeez Khan, Nicolas Galazis, Nikolina Docheva, Robert Layfield, William Atiomo
STUDY QUESTION: Do any proteomic biomarkers previously identified for pre-eclampsia (PE) overlap with those identified in women with polycystic ovary syndrome (PCOS). SUMMARY ANSWER: Five previously identified proteomic biomarkers were found to be common in women with PE and PCOS when compared with controls. WHAT IS KNOWN ALREADY: Various studies have indicated an association between PCOS and PE; however, the pathophysiological mechanisms supporting this association are not known...
January 2015: Human Reproduction
https://www.readbyqxmd.com/read/25268621/a-proteomic-analysis-of-human-follicular-fluid-comparison-between-younger-and-older-women-with-normal-fsh-levels
#9
Mahmoud Hashemitabar, Maryam Bahmanzadeh, Ali Mostafaie, Mahmoud Orazizadeh, Marzieh Farimani, Roshan Nikbakht
The follicular fluid (FF) is produced during folliculogenesis and contains a variety of proteins that play important roles in follicle development and oocyte maturation. Age-related infertility is usually considered as a problem that can be solved by assisted reproduction technology. Therefore, the identification of novel biomarkers that are linked to reproductive aging is the subject of this study. FF was obtained from healthy younger (20-32 years old) and older (38-42 years old) women undergoing intracytoplasmic sperm injection (ICSI) due to male factor infertility...
2014: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/24900998/power-of-proteomics-in-linking-oxidative-stress-and-female-infertility
#10
REVIEW
Sajal Gupta, Jana Ghulmiyyah, Rakesh Sharma, Jacques Halabi, Ashok Agarwal
Endometriosis, PCOS, and unexplained infertility are currently the most common diseases rendering large numbers of women infertile worldwide. Oxidative stress, due to its deleterious effects on proteins and nucleic acids, is postulated to be the one of the important mechanistic pathways in differential expression of proteins and in these diseases. The emerging field of proteomics has allowed identification of proteins involved in cell cycle, as antioxidants, extracellular matrix (ECM), cytoskeleton, and their linkage to oxidative stress in female infertility related diseases...
2014: BioMed Research International
https://www.readbyqxmd.com/read/24715425/effects-of-liuwei-dihuang-granule-symbols-see-text-on-the-outcomes-of-in-vitro-fertilization-pre-embryo-transfer-in-infertility-women-with-kidney-yin-deficiency-syndrome-and-the-proteome-expressions-in-the-follicular-fluid
#11
RANDOMIZED CONTROLLED TRIAL
Fang Lian, Hai-cui Wu, Zhen-gao Sun, Ying Guo, Lei Shi, Ming-yue Xue
OBJECTIVE: To observe the effects of Liuwei Dihuang Granule ([symbols; see text], LDG) for tonifying Kidney (Shen) on the outcomes of in vitro fertilization pre-embryo transfer (IVF-ET) of infertility women with Kidney-yin deficiency syndrome and to explore its mechanism by detecting the proteome expression in the follicular fluid. METHODS: Sixty-six infertility patients of Kidney-yin deficiency syndrome who would undergo IVF-ET, were randomly assigned to a treatment group and a control group according to a random number table, 33 cases in each group...
July 2014: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/24146976/an-integrated-approach-based-on-multiplexed-protein-array-and-itraq-labeling-for-in-depth-identification-of-pathways-associated-to-ivf-outcome
#12
Valeria Severino, Livia Malorni, Anna Emilia Cicatiello, Vittoria D'Esposito, Salvatore Longobardi, Nicola Colacurci, Nadia Miraglia, Nicola Sannolo, Annarita Farina, Angela Chambery
The emergence of high-throughput protein quantification methodologies has enabled the comprehensive characterization by longitudinal and cross-sectional studies of biological fluids under physiological and pathological conditions. In particular, the simultaneous investigation of cytokines and growth factors signaling pathways and their associated downstream effectors by integrated multiplexed approaches offers a powerful strategy to gain insights into biological networks and processes in living systems. A growing body of research indicates that bioactive molecules of human reproductive fluids, including human follicular fluid (hFF), may affect oocyte quality, fertilization and embryo development, thus potentially influencing the physiopathology of pregnancy-related conditions...
2013: PloS One
https://www.readbyqxmd.com/read/23888310/a-proteomic-analysis-of-human-follicular-fluid-comparison-between-fertilized-oocytes-and-non-fertilized-oocytes-in-the-same-patient
#13
Bayasula, Akira Iwase, Hiroharu Kobayashi, Maki Goto, Tatsuo Nakahara, Tomoko Nakamura, Mika Kondo, Yoshinari Nagatomo, Tomomi Kotani, Fumitaka Kikkawa
PURPOSE: Human follicular fluid constitutes the microenvironment of follicles and includes various biological active proteins that can affect follicle growth and oocyte fertilization. Conducting proteomic evaluations of human follicular fluid may be helpful for identifying potential biomarkers possibly possessing a predictive value for oocyte quality and the success of in vitro fertilization. METHOD: We performed proteomic profiling of human follicular fluids containing oocytes that were fertilized and resulted in pregnancy and follicular fluids containing oocytes that were not fertilized in the same patients undergoing intracytoplasmic sperm injection using the LTQ Orbitrap coupled with liquid chromatography-tandem mass spectrometry (LC/MS/MS) analyses...
September 2013: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/23707233/proteomic-analysis-of-human-follicular-fluid-a-new-perspective-towards-understanding-folliculogenesis
#14
Aditi S Ambekar, Raja Sekhar Nirujogi, Srinivas M Srikanth, Sandip Chavan, Dhanashree S Kelkar, Indira Hinduja, Kusum Zaveri, T S Keshava Prasad, H C Harsha, Akhilesh Pandey, Srabani Mukherjee
UNLABELLED: Human follicular fluid is a complex body fluid that constitutes the microenvironment of developing follicles in the ovary. Follicular fluid contains a number of proteins that modulate oocyte maturation and ovulation. Information about the protein constituents of follicular fluid may provide a better understanding of ovarian physiology in addition to opening new avenues for investigating ovarian disorders. However, the composition of follicular fluid proteome remains poorly defined...
July 11, 2013: Journal of Proteomics
https://www.readbyqxmd.com/read/23500131/a-methodological-and-functional-proteomic-approach-of-human-follicular-fluid-en-route-for-oocyte-quality-evaluation
#15
L Bianchi, A Gagliardi, G Campanella, C Landi, A Capaldo, A Carleo, A Armini, V De Leo, P Piomboni, R Focarelli, L Bini
UNLABELLED: Human follicular fluid (HFF) has been proven to contain biologically active molecules and proteins that may affect follicle growth and oocyte fertilization. Based on this concept, HFF proteomic characterization is having a significant impact in the delineation of a biomarkers' profile for oocyte quality estimation and, maybe, for in vitro fertilization (IVF) success improvement. Follicular fluid is characterized by a vast protein complexity and a broad dynamic range of protein abundances that hinder its analysis...
September 2, 2013: Journal of Proteomics
https://www.readbyqxmd.com/read/23338533/apolipoprotein-a-iv-as-a-novel-gene-associated-with-polycystic-ovary-syndrome
#16
Yong-Soo Kim, Bon-Hee Gu, Bum-Chae Choi, Myung-Sun Kim, Sangjin Song, Ji-Hyun Yun, Mi Kyung Chung, Cheol-Hee Choi, Kwang-Hyun Baek
Polycystic ovary syndrome (PCOS) is a common endocrine-metabolic disorder, affecting 6-10% of women of reproductive age. The etiology remains poorly understood. To investigate the differentially expressed proteins from PCOS patients versus healthy women, the protein expression in follicular fluid was analyzed using two-dimensional electrophoresis (2-DE). Since follicular fluid contains a number of secretory proteins required for oocyte fertilization and follicle maturation, it is possible that follicular fluid can be used as a provisional source for identifying pivotal proteins associated with PCOS...
March 2013: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/22988950/protein-and-steroid-profiles-in-follicular-fluid-after-ovarian-hyperstimulation-as-potential-biomarkers-of-ivf-outcome
#17
Mark M Kushnir, Tord Naessén, Kjell Wanggren, Alan L Rockwood, David K Crockett, Jonas Bergquist
Controlled ovarian hyperstimulation is performed to assist with generation of multiple mature oocytes for use in in vitro fertilization (IVF). The goal of our study was to evaluate differences in protein and steroid profiles in ovarian follicular fluid (hFF) samples obtained during oocyte retrieval from women undergoing IVF treatment and to identify physiological pathways associated with the proteins. The hFF samples were depleted of abundant proteins, fractionated by ultrafiltration, digested, and analyzed by nano-LC-QTOF...
October 5, 2012: Journal of Proteome Research
https://www.readbyqxmd.com/read/22935150/different-protein-expression-patterns-associated-with-polycystic-ovary-syndrome-in-human-follicular-fluid-during-controlled-ovarian-hyperstimulation
#18
Guo Dai, Guangxiu Lu
Polycystic ovary syndrome (PCOS) is one of the most common causes of anovulatory infertility, affecting 5-10% of females during their reproductive life. Currently the pathology of PCOS is largely unknown. To identify the differential protein expression in follicular fluids from PCOS and normal subjects during controlled ovarian hyperstimulation, we performed an initial proteomic study including two-dimensional gel electrophoresis (2DE) analysis and mass spectroscopy, and confirmed results by western blot. Thirty-two protein spots were shown to be significantly differentially expressed between PCOS and normal follicular fluids, of which 20 unique proteins were identified to be associated with cellular metabolism and physiological processes; 13 of these proteins were upregulated while seven were downregulated in PCOS follicular fluids...
2012: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/22589193/proteomic-analysis-of-the-microenvironment-of-developing-oocytes
#19
John Twigt, Regine P Steegers-Theunissen, Karel Bezstarosti, Jeroen A A Demmers
We utilized a setup based on extensive pre-fractionation of proteolytic peptides and nanoflow reversed-phase LC-MS/MS to identify the (sub)proteome of human follicular fluid (FF). In this in-depth screen, 246 specific proteins were identified, the majority of which are involved in coagulation- and immune-response pathways. Our aim is to define a set of FF protein markers, which could predict oocyte quality.
May 2012: Proteomics
https://www.readbyqxmd.com/read/22050422/improvement-of-2d-page-resolution-of-human-porcine-and-canine-follicular-fluid-comparison-of-two-immunodepletion-columns
#20
COMPARATIVE STUDY
S Fahiminiya, S Roche, N Gérard
Follicular fluid provides the microenvironment within which somatic cells proliferate and differentiate, and the oocyte matures. It contains a number of soluble factors implicated in various stages of follicular development, most of them being functionally unknown. The presence of several high-abundance proteins, mainly originating from the blood circulation, is a major challenge of follicular fluid proteomic analysis, as these proteins can mask or decrease the visualization of follicle-specific proteins. In this study, we evaluated the efficiency of two immunodepletion columns (ProteomeLab™ IgY-HSA and MARS-6) on follicular fluids of human, porcine and canine prior to 2D-PAGE...
October 2012: Reproduction in Domestic Animals, Zuchthygiene
keyword
keyword
112524
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"