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Proteomic follicular fluid

Aleksandra E Lewandowska, Katarzyna Macur, Paulina Czaplewska, Joanna Liss, Krzysztof Łukaszuk, Stanisław Ołdziej
Analysis of proteomic composition of human follicular fluid (hFF) has been previously proposed as a potential tool of oocyte quality evaluation. In order to develop an efficient method to investigate the hFF proteome and peptidome components, we applied and tested a few prefractionation schemes of hFF material consisting of ultrafiltration, optional immunodepletion, and high pH RP-HPLC separation by building spectral libraries and comparing their quantification capabilities of unfractionated samples. Low Molecular-Weight Fraction peptides (LMWF, <10 kDa) and High Molecular-Weight Fraction proteins (HMWF, >10 kDa) resulting from ultrafiltration were analyzed separately...
March 10, 2018: Journal of Proteomics
Alexandre R Paula Junior, Mauricio F van Tilburg, Marina D P Lobo, Ana C O Monteiro-Moreira, Renato A Moreira, Carlos H S Melo, Joanna M G Souza-Fabjan, Airton A Araújo, Luciana M Melo, Dárcio I A Teixeira, Arlindo A Moura, Vicente J F Freitas
The present study was conducted to characterize the major proteome of ovarian follicular fluid from locally-adapted, "Canindé" goats in the northeast of Brazil. Eight estrous cycling goats received a hormonal treatment consisting of medroxyprogesterone acetate, D-cloprostenol and FSH. Fluid was collected by laparoscopy from small (<3mm), medium (3-4mm) and large (>4mm) follicles and then, proteins were analyzed by 2-D SDS-PAGE and tandem mass spectrometry. Thirty-six proteins were identified in the goat follicular fluid, including albumin, immunoglobulins, ceruloplasmin, complement factor B, alpha-1B-glycoprotein precursor, serotransferrin, complement C3 and serpins, among others...
January 2018: Animal Reproduction Science
Rodrigo de Andrade Ferrazza, Henry David Mogollón Garcia, Elizabeth Moreira Dos Santos Schmidt, Monika Mihm Carmichael, Fabiana Ferreira de Souza, Richard Burchmore, Roberto Sartori, Peter David Eckersall, João Carlos Pinheiro Ferreira
Bovine follicular fluid (FF) constitutes the microenvironment of follicles and includes various biologically active proteins. We performed a study involving 18 healthy nonlactating Holstein cows to determine the protein expression profile of FF at key stages of follicular development. Follicles were individually aspirated in vivo at predeviation (F1 ∼ 7.0 mm), deviation (F1 ∼ 8.5 mm), postdeviation (F1 ∼ 12.0 mm), and preovulatory stages of follicle development, which were confirmed by measurement of follicular estradiol and progesterone concentrations...
January 1, 2017: Biology of Reproduction
Y Wu, J Lin, B Han, L Wang, Y Chen, M Liu, J Huang
Follicular fluid (FF) accumulates in the antrum of the ovarian follicle. In addition, FF provides the microenvironment for oocyte development, oocyte maturation and competence, which are acquired during follicular development. Superstimulatory treatment of 1-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles are unable to completely mature and ovulate. Furthermore, the oocytes exhibit lower competence compared with those of ewes. In this study, we utilized an isobaric tag for relative and absolute quantification (iTRAQ)-based proteomics analysis and compare protein composition between pre-pubertal and adult superstimulated follicle FF in sheep...
February 2018: Reproduction in Domestic Animals, Zuchthygiene
Hee-Joung Lim, Ae Eun Seok, Jiyou Han, Jiyeong Lee, Sungeun Lee, Hee-Gyoo Kang, Byung Heun Cha, Yunseok Yang
OBJECTIVE: Hyperstimulation methods are broadly used for in vitro fertilization (IVF) in patients with infertility; however, the side effects associated with these therapies, such as ovarian hyperstimulation syndrome (OHSS), have not been well studied. N-glycoproteomes are subproteomes used for the remote sensing of ovarian stimulation in follicular growth. Glycoproteomic variation in human follicular fluid (hFF) has not been evaluated. In this study, we aimed to identify and quantify the glycoproteomes and N-glycoproteins (N-GPs) in natural and stimulated hFF using label-free nano-liquid chromatography/electrospray ionization-quad time-of-flight mass spectrometry...
June 2017: Clinical and Experimental Reproductive Medicine
Xiaofang Shen, Xin Liu, Peng Zhu, Yuhua Zhang, Jiahui Wang, Yanwei Wang, Wenting Wang, Juan Liu, Ning Li, Fujun Liu
BACKGROUND: Human follicular fluid (HFF) provides a key environment for follicle development and oocyte maturation, and contributes to oocyte quality and in vitro fertilization (IVF) outcome. METHODS: To better understand folliculogenesis in the ovary, a proteomic strategy based on dual reverse phase high performance liquid chromatography (RP-HPLC) coupled to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (LC-MALDI TOF/TOF MS) was used to investigate the protein profile of HFF from women undergoing successful IVF...
July 27, 2017: Reproductive Biology and Endocrinology: RB&E
Aleksandra E Lewandowska, Katarzyna Macur, Paulina Czaplewska, Joanna Liss, Krzysztof Łukaszuk, Stanisław Ołdziej
Human follicular fluid (hFF) is a natural environment of oocyte maturation, and some components of hFF could be used to judge oocyte capability for fertilization and further development. In our pilot small-scale study three samples from four donors (12 samples in total) were analyzed to determine which hFF proteins/peptides could be used to differentiate individual oocytes and which are patient-specific. Ultrafiltration was used to fractionate hFF to high-molecular-weight (HMW) proteome (>10 kDa) and low-molecular-weight (LMW) peptidome (<10 kDa) fractions...
August 4, 2017: Journal of Proteome Research
Saranya Satitmanwiwat, Chinarat Changsangfah, Tassanee Faisaikarm, Kornkanok Promthep, Sujira Thammawung, Kulnasan Saikhun, Kampon Kaeoket
The objective of this study was to determine the effect of bovine follicular fluid proteins (bFF) and their differently charged groups as maturation media supplements for in vitro embryo development. bFF was obtained by aspiration from large healthy follicles (4-10 mm in diameter) and was precipitated by 30-50% (NH4)2SO4. The precipitated protein was fractionated into basic and acidic fractions by ion-exchanger columns. In experiment 1, the oocytes were matured in TCM-199 with 1) FBS+hormones (control) and 2) 10% bFF...
May 3, 2017: Journal of Veterinary Medical Science
Jae Won Oh, Seul Ki Kim, Kyung-Cho Cho, Min-Sik Kim, Chang Suk Suh, Jung Ryeol Lee, Kwang Pyo Kim
Poor ovarian response (POR) in controlled ovarian stimulation is often observed during in vitro fertilization and embryo transfer cycles and it is a major problem. A POR has been found to be related to several factors, including advanced age, high body mass index, and history of ovarian or pelvic surgery. However, it is difficult to predict POR, as there are no specific biomarkers known. In this study, we used quantitative proteomic analyses to investigate potential biomarkers that can predict poor response during in vitro fertilization based on follicular fluid samples...
March 2017: Proteomics
Maya Zachut, Pankaj Sood, Lilya Livshitz, Gitit Kra, Yishai Levin, Uzi Moallem
This article contains raw and processed data related to research published in Zachut et al. (2016) [1]. Proteomics data from preovulatory follicles in cows was obtained by liquid chromatography-mass spectrometry following protein extraction. Differential expression between controls and less fertile cows (LFC) was quantified using MS1 intensity based label-free. The only previous proteomic analysis of bovine FF detected merely 40 proteins in follicular cysts obtained from the slaughterhouse (Maniwa et al., 2005) [2], and the abundance of proteins in the bovine preovulatory FF remains unknown...
June 2016: Data in Brief
Federica Ciregia, Laura Giusti, Angelo Molinaro, Filippo Niccolai, Maria R Mazzoni, Teresa Rago, Massimo Tonacchera, Paolo Vitti, Gino Giannaccini, Antonio Lucacchini
Thyroid nodules are common in the general population and vary widely in their propensity to harbor thyroid malignancies. The category of follicular lesion of undetermined significance, for instance, carries only a 15% risk of malignancy. The overarching aim of this work was the proteomic study of thyroid cancer because more effort needs to be placed on differentiating malignant thyroid nodules to avoid unnecessary thyroidectomy. We used 2-dimensional electrophoresis coupled to nano-liquid chromatography electrospray ionization tandem mass spectrometry, to examine fine-needle aspiration (FNA), which was easily attainable from the wash of the syringe used for classical FNA biopsy...
October 2016: Translational Research: the Journal of Laboratory and Clinical Medicine
Qiang Fu, Yulin Huang, Zhiqiang Wang, Fumei Chen, Delun Huang, Yangqing Lu, Xianwei Liang, Ming Zhang
Follicular fluid (FF) accumulates in the antrum of the ovarian follicle and provides the microenvironment for oocyte development. FF plays an important role in follicle growth and oocyte maturation. The FF provides a unique window to investigate the processes occurring during buffalo follicular development. The observed low quality of buffalo oocytes may arise from the poor follicular microenvironment. Investigating proteins found in buffalo FF (BFF) should provide insight into follicular development processes and provide further understanding of intra-follicular maturation and oocytes quality...
April 29, 2016: International Journal of Molecular Sciences
Fabio Pagni, Gabriele De Sio, Mattia Garancini, Marcella Scardilli, Clizia Chinello, Andrew James Smith, Francesca Bono, Davide Leni, Fulvio Magni
Several proteomic strategies are used extensively for the purpose of biomarker discovery and in order to obtain insights into the molecular aspects of cancers, using either body fluids or tissue as samples. Among them, MALDI-imaging can be applied to cytological thyroid specimens to investigate the molecular signatures of different pathological conditions and highlight differences in the proteome that are of relevance for diagnostic and pathogenetic research. In this study, 26 ex-vivo fine needle aspirations from benign thyroid nodules (n = 13) and papillary thyroid carcinomas (n = 13) were analyzed by MALDI-imaging...
June 2016: Proteomics
Katrina Laks, Tiina Kirsipuu, Tuuli Dmitrijeva, Andres Salumets, Peep Palumaa
Biological fluid sample collection often includes the risk of blood contamination that may alter the proteomic profile of biological fluid. In proteomics studies, exclusion of contaminated samples is usually based on visual inspection and counting of red blood cells in the sample; analysis of specific blood derived proteins is less used. To fill the gap, we developed a fast and sensitive method for ascertainment of blood contamination in crude biological fluids, based on specific blood-derived protein, hemoglobin detection by MALDI-TOF MS...
June 2016: Protein Journal
Maya Zachut, Pankaj Sood, Yishai Levin, Uzi Moallem
UNLABELLED: The follicular fluid (FF) proteome can provide an indication of follicular quality. High-yielding dairy cows suffer from low fertility, which could be related to follicular function. However, the proteome of preovulatory follicles has never been described in cows. Our objectives were to: 1) define the bovine preovulatory FF proteome, and 2) examine differentially abundant proteins in FF of controls (CTL, n=10) and less fertile cows (LFC; failed to conceive following ≥6 inseminations, n=8)...
April 29, 2016: Journal of Proteomics
Xian Guo, Jie Pei, Xuezhi Ding, Min Chu, Pengjia Bao, Xiaoyun Wu, Chunnian Liang, Ping Yan
The breeding of yaks is highly seasonal, there are many crucial proteins involved in the reproduction control program, especially in follicular development. In order to isolate differential proteins between mature and immature follicular fluid (FF) of yak, the FF from yak follicles with different sizes were sampled respectively, and two-dimensional gel electrophoresis (2-DE) of the proteins was carried out. After silver staining, the Image Master 2D platinum software was used for protein analysis and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was performed for differential protein identification...
September 2016: Asian-Australasian Journal of Animal Sciences
Elisa Giacomini, Ana M Sanchez, Veronica Sarais, Soha Al Beitawi, Massimo Candiani, Paola Viganò
The study of follicular fluid (FF) content nearby endometriomas may assist in elucidating pathophysiology, possible biomarkers related to this disease and the effect of endometriomas on ovarian physiology. As the question "how endometrioma may intrude the physiology of ovarian tissue?" is still open, we aimed to summarize the molecular evidence supporting the idea that endometriomas can negatively influence the content of the surrounding ovarian follicles. An alteration of the iron metabolism and an increased ROS (reactive oxygen species) generation characterize the intrafollicular environment adjacent to endometriomas...
February 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Thais Regiani, Fernanda Bertuccez Cordeiro, Lívia do Vale Teixeira da Costa, Jéssica Salgueiro, Karina Cardozo, Valdemir Melechco Carvalho, Kayla Jane Perkel, Daniel Suslik Zylbersztejn, Agnaldo Pereira Cedenho, Edson Guimarães Lo Turco
Endometriosis is a chronic gynecological condition that affects 10-32% of women of reproductive age and may lead to infertility. The study of protein profiles in follicular fluid may assist in elucidating possible biomarkers related to this disease. For this, follicular fluid samples were obtained from women with tubal factor or minimal male factor infertility who had pregnancy outcomes after in vitro fertilization (IVF) treatment (control group, n = 10), women with endometriosis (endometriosis group, n = 10), along with the endometrioma from these same patients were included (endometrioma group, n = 10)...
2015: Systems Biology in Reproductive Medicine
Moncef Benkhalifa, Aicha Madkour, Noureddine Louanjli, Nouzha Bouamoud, Brahim Saadani, Ismail Kaarouch, Hikmat Chahine, Omar Sefrioui, Philippe Merviel, Henri Copin
The development of in vitro fertilization (IVF) techniques for infertility management has led to the investigation of the proteome of follicular fluid and oocyte. In addition, different markers contributing to oocyte maturation and embryo development potential have been reported in the literature. Different techniques were utilized to analyze whole proteome or single protein markers in follicular fluid and oocytes, particularly in animal models. Data from several studies have generated large amounts of information, however, an ideal profile to predict the best oocytes and embryos suitable for implantation are still to be uncovered...
August 2015: Expert Review of Proteomics
John M Twigt, Karel Bezstarosti, Jeroen Demmers, Jan Lindemans, Joop S E Laven, Régine P Steegers-Theunissen
BACKGROUND: The investigation of the human follicle fluid proteome has gained much interest in the search of new markers as predictors for in vitro fertilization and intracytoplasmic sperm injection (IVF/ICSI) treatment outcome. Follicular fluid folate, as substrate of one carbon (1-C) metabolism, affects follicular metabolism and oocyte and embryo quality. From this background, we aim to identify a folate-related follicle fluid proteome that associates with IVF/ICSI treatment outcome...
August 2015: European Journal of Clinical Investigation
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