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Proteomic follicular fluid

Saranya Satitmanwiwat, Chinarat Changsangfah, Tassanee Faisaikarm, Kornkanok Promthep, Sujira Thammawung, Kulnasan Saikhun, Kampon Kaeoket
The objective of this study was to determine the effect of bovine follicular fluid proteins (bFF) and their differently charged groups as maturation media supplements for in vitro embryo development. bFF was obtained by aspiration from large healthy follicles (4-10 mm in diameter) and was precipitated by 30-50% (NH4)2SO4. The precipitated protein was fractionated into basic and acidic fractions by ion-exchanger columns. In experiment 1, the oocytes were matured in TCM-199 with 1) FBS+hormones (control) and 2) 10% bFF...
March 3, 2017: Journal of Veterinary Medical Science
Jae Won Oh, Seul Ki Kim, Kyung-Cho Cho, Min-Sik Kim, Chang Suk Suh, Jung Ryeol Lee, Kwang Pyo Kim
Poor ovarian response (POR) observed in controlled ovarian stimulation (COS) is often observed during in vitro fertilization and embryo transfer (IVF-ET) cycles and is a major problem. A POR has been found to be related to several factors, including advanced age, high body mass index, and history of ovarian or pelvic surgery. However, it is difficult to predict POR, as there are no specific biomarkers known. In this study, we used quantitative proteomic analyses to investigate potential biomarkers that can predict poor response during IVF based on follicular fluid samples...
January 28, 2017: Proteomics
Maya Zachut, Pankaj Sood, Lilya Livshitz, Gitit Kra, Yishai Levin, Uzi Moallem
This article contains raw and processed data related to research published in Zachut et al. (2016) [1]. Proteomics data from preovulatory follicles in cows was obtained by liquid chromatography-mass spectrometry following protein extraction. Differential expression between controls and less fertile cows (LFC) was quantified using MS1 intensity based label-free. The only previous proteomic analysis of bovine FF detected merely 40 proteins in follicular cysts obtained from the slaughterhouse (Maniwa et al., 2005) [2], and the abundance of proteins in the bovine preovulatory FF remains unknown...
June 2016: Data in Brief
Federica Ciregia, Laura Giusti, Angelo Molinaro, Filippo Niccolai, Maria R Mazzoni, Teresa Rago, Massimo Tonacchera, Paolo Vitti, Gino Giannaccini, Antonio Lucacchini
Thyroid nodules are common in the general population and vary widely in their propensity to harbor thyroid malignancies. The category of follicular lesion of undetermined significance, for instance, carries only a 15% risk of malignancy. The overarching aim of this work was the proteomic study of thyroid cancer because more effort needs to be placed on differentiating malignant thyroid nodules to avoid unnecessary thyroidectomy. We used 2-dimensional electrophoresis coupled to nano-liquid chromatography electrospray ionization tandem mass spectrometry, to examine fine-needle aspiration (FNA), which was easily attainable from the wash of the syringe used for classical FNA biopsy...
October 2016: Translational Research: the Journal of Laboratory and Clinical Medicine
Qiang Fu, Yulin Huang, Zhiqiang Wang, Fumei Chen, Delun Huang, Yangqing Lu, Xianwei Liang, Ming Zhang
Follicular fluid (FF) accumulates in the antrum of the ovarian follicle and provides the microenvironment for oocyte development. FF plays an important role in follicle growth and oocyte maturation. The FF provides a unique window to investigate the processes occurring during buffalo follicular development. The observed low quality of buffalo oocytes may arise from the poor follicular microenvironment. Investigating proteins found in buffalo FF (BFF) should provide insight into follicular development processes and provide further understanding of intra-follicular maturation and oocytes quality...
April 29, 2016: International Journal of Molecular Sciences
Fabio Pagni, Gabriele De Sio, Mattia Garancini, Marcella Scardilli, Clizia Chinello, Andrew James Smith, Francesca Bono, Davide Leni, Fulvio Magni
Several proteomic strategies are used extensively for the purpose of biomarker discovery and in order to obtain insights into the molecular aspects of cancers, using either body fluids or tissue as samples. Among them, MALDI-imaging can be applied to cytological thyroid specimens to investigate the molecular signatures of different pathological conditions and highlight differences in the proteome that are of relevance for diagnostic and pathogenetic research. In this study, 26 ex-vivo fine needle aspirations from benign thyroid nodules (n = 13) and papillary thyroid carcinomas (n = 13) were analyzed by MALDI-imaging...
June 2016: Proteomics
Katrina Laks, Tiina Kirsipuu, Tuuli Dmitrijeva, Andres Salumets, Peep Palumaa
Biological fluid sample collection often includes the risk of blood contamination that may alter the proteomic profile of biological fluid. In proteomics studies, exclusion of contaminated samples is usually based on visual inspection and counting of red blood cells in the sample; analysis of specific blood derived proteins is less used. To fill the gap, we developed a fast and sensitive method for ascertainment of blood contamination in crude biological fluids, based on specific blood-derived protein, hemoglobin detection by MALDI-TOF MS...
June 2016: Protein Journal
Maya Zachut, Pankaj Sood, Yishai Levin, Uzi Moallem
UNLABELLED: The follicular fluid (FF) proteome can provide an indication of follicular quality. High-yielding dairy cows suffer from low fertility, which could be related to follicular function. However, the proteome of preovulatory follicles has never been described in cows. Our objectives were to: 1) define the bovine preovulatory FF proteome, and 2) examine differentially abundant proteins in FF of controls (CTL, n=10) and less fertile cows (LFC; failed to conceive following ≥6 inseminations, n=8)...
April 29, 2016: Journal of Proteomics
Xian Guo, Jie Pei, Xuezhi Ding, Min Chu, Pengjia Bao, Xiaoyun Wu, Chunnian Liang, Ping Yan
The breeding of yaks is highly seasonal, there are many crucial proteins involved in the reproduction control program, especially in follicular development. In order to isolate differential proteins between mature and immature follicular fluid (FF) of yak, the FF from yak follicles with different sizes were sampled respectively, and two-dimensional gel electrophoresis (2-DE) of the proteins was carried out. After silver staining, the Image Master 2D platinum software was used for protein analysis and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was performed for differential protein identification...
September 2016: Asian-Australasian Journal of Animal Sciences
Elisa Giacomini, Ana M Sanchez, Veronica Sarais, Soha Al Beitawi, Massimo Candiani, Paola Viganò
The study of follicular fluid (FF) content nearby endometriomas may assist in elucidating pathophysiology, possible biomarkers related to this disease and the effect of endometriomas on ovarian physiology. As the question "how endometrioma may intrude the physiology of ovarian tissue?" is still open, we aimed to summarize the molecular evidence supporting the idea that endometriomas can negatively influence the content of the surrounding ovarian follicles. An alteration of the iron metabolism and an increased ROS (reactive oxygen species) generation characterize the intrafollicular environment adjacent to endometriomas...
February 8, 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Thais Regiani, Fernanda Bertuccez Cordeiro, Lívia do Vale Teixeira da Costa, Jéssica Salgueiro, Karina Cardozo, Valdemir Melechco Carvalho, Kayla Jane Perkel, Daniel Suslik Zylbersztejn, Agnaldo Pereira Cedenho, Edson Guimarães Lo Turco
Endometriosis is a chronic gynecological condition that affects 10-32% of women of reproductive age and may lead to infertility. The study of protein profiles in follicular fluid may assist in elucidating possible biomarkers related to this disease. For this, follicular fluid samples were obtained from women with tubal factor or minimal male factor infertility who had pregnancy outcomes after in vitro fertilization (IVF) treatment (control group, n = 10), women with endometriosis (endometriosis group, n = 10), along with the endometrioma from these same patients were included (endometrioma group, n = 10)...
2015: Systems Biology in Reproductive Medicine
Moncef Benkhalifa, Aicha Madkour, Noureddine Louanjli, Nouzha Bouamoud, Brahim Saadani, Ismail Kaarouch, Hikmat Chahine, Omar Sefrioui, Philippe Merviel, Henri Copin
The development of in vitro fertilization (IVF) techniques for infertility management has led to the investigation of the proteome of follicular fluid and oocyte. In addition, different markers contributing to oocyte maturation and embryo development potential have been reported in the literature. Different techniques were utilized to analyze whole proteome or single protein markers in follicular fluid and oocytes, particularly in animal models. Data from several studies have generated large amounts of information, however, an ideal profile to predict the best oocytes and embryos suitable for implantation are still to be uncovered...
August 2015: Expert Review of Proteomics
John M Twigt, Karel Bezstarosti, Jeroen Demmers, Jan Lindemans, Joop S E Laven, Régine P Steegers-Theunissen
BACKGROUND: The investigation of the human follicle fluid proteome has gained much interest in the search of new markers as predictors for in vitro fertilization and intracytoplasmic sperm injection (IVF/ICSI) treatment outcome. Follicular fluid folate, as substrate of one carbon (1-C) metabolism, affects follicular metabolism and oocyte and embryo quality. From this background, we aim to identify a folate-related follicle fluid proteome that associates with IVF/ICSI treatment outcome...
August 2015: European Journal of Clinical Investigation
Hans Dieplinger, Benjamin Dieplinger
The human glycoprotein afamin was discovered as the fourth member of the albumin gene family. Despite intense research over the last 20 years, our knowledge of afamin's physiological or pathophysiological functions is still very limited. Circulating afamin is primarily of hepatic origin and abundant concentrations are found in plasma, cerebrospinal, ovarian follicular and seminal fluids. In vitro binding studies revealed specific binding properties for vitamin E. A previously performed analytical characterization and clinical evaluation study of an enzyme-linked immunosorbent assay for quantitative measurement of afamin in human plasma demonstrated that the afamin assay meets the quality specifications for laboratory medicine...
June 15, 2015: Clinica Chimica Acta; International Journal of Clinical Chemistry
Alberuni M Zamah, Maria E Hassis, Matthew E Albertolle, Katherine E Williams
BACKGROUND: Follicular fluid is a unique biological fluid in which the critical events of oocyte and follicular maturation and somatic cell-germ cell communication occur. Because of the intimate proximity of follicular fluid to the maturing oocyte, this fluid provides a unique window into the processes occurring during follicular maturation. A thorough identification of the specific components within follicular fluid may provide a better understanding of intrafollicular signaling, as well as reveal potential biomarkers of oocyte health for women undergoing assisted reproductive treatment...
2015: Clinical Proteomics
Yan-Ting Wu, Yan Wu, Jun-Yu Zhang, Ning-Ning Hou, Ai-Xia Liu, Jie-Xue Pan, Jie-Yang Lu, Jian-Zhong Sheng, He-Feng Huang
PURPOSE: To study the differences in protein expression profiles of follicular fluid (FF) between controlled ovarian hyperstimulation (COH) and natural ovulatory cycles. METHODS: Twelve infertile women undergoing in vitro fertilization and embryo transfer (IVF-ET), with matched clinical information, were retrospectively recruited in the IVF center of our university hospital, including six undergoing COH and another six with natural cycles. FF was sampled from dominant follicles with mature oocytes...
March 2015: Journal of Assisted Reproduction and Genetics
Joanna Nynca, Georg J Arnold, Thomas Fröhlich, Andrzej Ciereszko
In the present study we used a shotgun proteomic approach to identify 54 proteins of rainbow trout ovarian fluid. The study has unravelled the identity of several proteins not previously reported in fish ovarian fluid. The proteome of trout ovarian fluid consists of diverse proteins participating in lipid binding and metabolism, carbohydrate and ion transport, innate immunity, maturation and ovulation processes. Most trout ovarian fluid proteins correspond to follicular fluid proteins of higher vertebrates, but 15% of the proteins were found to be different, such as those related to the immune system (precerebellin-like protein), proteolysis (myeloid cell lineage chitinase), carbohydrate and lipid binding and metabolism (vitellogenins), cell structure and shape (vitelline envelope protein gamma) and a protein with unknown functions (UPF0762 protein C6orf58 homologue)...
March 2015: Reproduction, Fertility, and Development
Aditi S Ambekar, Dhanashree S Kelkar, Sneha M Pinto, Rakesh Sharma, Indira Hinduja, Kusum Zaveri, Akhilesh Pandey, T S Keshava Prasad, Harsha Gowda, Srabani Mukherjee
CONTEXT: Polycystic ovary syndrome (PCOS), a major cause of anovulatory infertility, is characterized by arrested follicular growth. Altered protein levels in the follicular fluid surrounding the ovum may reflect the molecular defects of folliculogenesis in these women. OBJECTIVE: To identify differentially regulated proteins in PCOS by comparing the follicular fluid protein repertoire of PCOS with healthy women. METHODS: The follicular fluid samples were collected from PCOS and normo-ovulatory women undergoing in vitro fertilization...
February 2015: Journal of Clinical Endocrinology and Metabolism
Gulafshana Hafeez Khan, Nicolas Galazis, Nikolina Docheva, Robert Layfield, William Atiomo
STUDY QUESTION: Do any proteomic biomarkers previously identified for pre-eclampsia (PE) overlap with those identified in women with polycystic ovary syndrome (PCOS). SUMMARY ANSWER: Five previously identified proteomic biomarkers were found to be common in women with PE and PCOS when compared with controls. WHAT IS KNOWN ALREADY: Various studies have indicated an association between PCOS and PE; however, the pathophysiological mechanisms supporting this association are not known...
January 2015: Human Reproduction
Mahmoud Hashemitabar, Maryam Bahmanzadeh, Ali Mostafaie, Mahmoud Orazizadeh, Marzieh Farimani, Roshan Nikbakht
The follicular fluid (FF) is produced during folliculogenesis and contains a variety of proteins that play important roles in follicle development and oocyte maturation. Age-related infertility is usually considered as a problem that can be solved by assisted reproduction technology. Therefore, the identification of novel biomarkers that are linked to reproductive aging is the subject of this study. FF was obtained from healthy younger (20-32 years old) and older (38-42 years old) women undergoing intracytoplasmic sperm injection (ICSI) due to male factor infertility...
2014: International Journal of Molecular Sciences
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