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https://www.readbyqxmd.com/read/28239514/a-fragmented-adeno-associated-viral-dual-vector-strategy-for-treatment-of-diseases-caused-by-mutations-in-large-genes-leads-to-expression-of-hybrid-transcripts
#1
Michelle E McClements, Peter Charbel Issa, Véronique Blouin, Robert E MacLaren
OBJECTIVE: Dual vector AAV systems are being utilised to enable gene therapy for disorders in which the disease gene is too large to fit into a single capsid. Fragmented adeno-associated viral (fAAV) vectors containing single inverted terminal repeat truncated transgenes have been considered as one such gene replacement strategy. Here we aim to add to the current understanding of the molecular mechanisms employed by fAAV dual vector systems. METHODS: Oversized (>8kb) transgene constructs containing ABCA4 coding sequence were packaged as truncated fragments <5kb in size into various AAV serotypes...
November 14, 2016: Journal of Genetic Syndrome & Gene Therapy
https://www.readbyqxmd.com/read/28235898/gabaergic-neurons-of-the-central-amygdala-promote-cataplexy
#2
Carrie E Mahoney, Lindsay J Agostinelli, Jessica N K Brooks, Bradford B Lowell, Thomas E Scammell
Narcolepsy is characterized by chronic sleepiness and cataplexy - sudden muscle paralysis triggered by strong, positive emotions. Narcolepsy is caused by a lack of orexin (hypocretin) signaling, but little is known about the neural mechanisms that mediate cataplexy. The amygdala regulates responses to rewarding stimuli and contains neurons active during cataplexy. In addition, lesions of the amygdala reduce cataplexy. As GABAergic neurons of the central nucleus of the amygdala (CeA) target brainstem regions known to regulate muscle tone, we hypothesized that these cells promote emotion-triggered cataplexy...
February 24, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28226022/-csapaih-a-function-to-classify-ambulatory-care-sensitive-conditions-in-the-statistical-software-r
#3
Fúlvio Borges Nedel
Hospitalizations due to ambulatory care sensitive conditions (ACSC) are an indirect indicator of primary health care. A package in the R program was developed in order to automatize the classification of codes of the International Statistical Classification of Diseases and Related Health Problems - 10th Revision (ICD-10), according to the Brazilian list of ACSC, and provide functionalities for the management of the "reduced files" of the inpatient hospital authorization (AIH). The csapAIH package contains a homonym function, which reads the data according to its nature (file or data frame with AIH structure, or a factor with ICD-10 codes) and returns, according to defined options, a databank or vector containing the classification for the hospitalization...
January 2017: Epidemiologia e Servicos de Saude: Revista do Sistema Unico de Saude do Brasil
https://www.readbyqxmd.com/read/28213950/benchmark-performance-of-global-switching-versus-local-switching-for-trajectory-surface-hopping-molecular-dynamic-simulation-cis-trans-azobenzene-photoisomerization
#4
Ling Yue, Le Yu, Chao Xu, Yibo Lei, Yajun Liu, Chaoyuan Zhu
Newly developed global switching algorithm that does not require calculation of nonadiabatic coupling vector reduces computational cost significantly. However, there is a lack of extensive checking its accuracy for this simplest nonadiabatic molecular dynamic method in comparison with conventional Tully's fewest switches. It is very necessary to demonstrate how accurate global switching algorithm is. Extensive comparison between local and global switching on-the-fly trajectory surface hopping molecular dynamics is performed for cis-to-trans (800 sampling trajectories) and trans-to-cis (600 sampling trajectories) azobenzene photoisomerization at the OM2/MRCI level...
February 18, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28209207/recombinant-%C3%AE-actinin-subunit-antigens-of-trichomonas-vaginalis-as-potential-vaccine-candidates-in-protecting-against-trichomoniasis
#5
Yi-Ting Xie, Jiang-Mei Gao, Ya-Ping Wu, Petrus Tang, Geoff Hide, De-Hua Lai, Zhao-Rong Lun
BACKGROUND: Human trichomoniasis caused by Trichomonas vaginalis is one of the most common sexually transmitted diseases with more than 200 million cases worldwide. It has caused a series of health problems to patients. For prevention and control of infectious diseases, vaccines are usually considered as one of the most cost-efficient tools. However, until now, work on the development of T. vaginalis vaccines is still mainly focused on the screening of potential immunogens. Alpha-actinin characterized by high immunogenicity in T...
February 16, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28199924/hip-ankle-coordination-during-gait-in-individuals-with-chronic-ankle-instability
#6
Sheng-Che Yen, Kevin K Chui, Marie B Corkery, Elizabeth A Allen, Caitlin M Cloonan
Individuals with chronic ankle instability (CAI) may have sensorimotor impairments that affect control at the hip in addition to the ankle. The purpose of this study was to compare hip-ankle coordination and coordination variability between individuals with CAI and healthy individuals during walking. Ten healthy subjects and 10 subjects with CAI were recruited to walk on a treadmill. Hip-ankle coordination was quantified using vector coding, and coordination variability was quantified using coefficient of correspondence...
February 7, 2017: Gait & Posture
https://www.readbyqxmd.com/read/28193277/anti-tumor-effects-of-a-recombinant-anti-prostate-specific-membrane-antigen-immunotoxin-against-prostate-cancer-cells
#7
Ping Meng, Qing-Chuan Dong, Guang-Guo Tan, Wei-Hong Wen, He Wang, Geng Zhang, Yan-Zhu Wang, Yu-Ming Jing, Chen Wang, Wei-Jun Qin, Jian-Lin Yuan
BACKGROUND: To evaluate anti-prostate cancer effects of a chimeric tumor-targeted killer protein. METHODS: We established a novel fusion gene, immunocasp-3, composed of NH2-terminal leader sequence fused with an anti-prostate-specific membrane antigen (PSMA) antibody (J591), the furin cleavage sequences of diphtheria toxin (Fdt), and the reverse coding sequences of the large and small subunits of caspase-3 (revcaspase-3). The expressing level of the immunocasp-3 gene was evaluated by using the reverse transcription-PCR (RT-PCR) and western blot analysis...
February 13, 2017: BMC Urology
https://www.readbyqxmd.com/read/28182719/context-adaptive-based-cu-processing-for-3d-hevc
#8
Liquan Shen, Ping An, Zhi Liu
The 3D High Efficiency Video Coding (3D-HEVC) standard aims to code 3D videos that usually contain multi-view texture videos and its corresponding depth information. It inherits the same quadtree prediction structure of HEVC to code both texture videos and depth maps. Each coding unit (CU) allows recursively splitting into four equal sub-CUs. At each CU depth level, it enables 10 types of inter modes and 35 types of intra modes in inter frames. Furthermore, the inter-view prediction tools are applied to each view in the test model of 3D-HEVC (HTM), which uses variable size disparity-compensated prediction to exploit inter-view correlation within neighbor views...
2017: PloS One
https://www.readbyqxmd.com/read/28179027/genome-scaffolding-and-annotation-for-the-pathogen-vector-ixodes-ricinus-by-ultra-long-single-molecule-sequencing
#9
Wibke J Cramaro, Oliver E Hunewald, Lesley Bell-Sakyi, Claude P Muller
BACKGROUND: Global warming and other ecological changes have facilitated the expansion of Ixodes ricinus tick populations. Ixodes ricinus is the most important carrier of vector-borne pathogens in Europe, transmitting viruses, protozoa and bacteria, in particular Borrelia burgdorferi (sensu lato), the causative agent of Lyme borreliosis, the most prevalent vector-borne disease in humans in the Northern hemisphere. To faster control this disease vector, a better understanding of the I...
February 8, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28173829/cloning-expression-and-molecular-characterization-of-a-cystoisospora-suis-specific-uncharacterized-merozoite-protein
#10
Aruna Shrestha, Nicola Palmieri, Ahmed Abd-Elfattah, Bärbel Ruttkowski, Marc Pagès, Anja Joachim
BACKGROUND: The genome of the apicomplexan parasite Cystoisospora suis (syn. Isospora suis) has recently been sequenced and annotated, opening the possibility for the identification of novel therapeutic targets against cystoisosporosis. It was previously proposed that a 42 kDa uncharacterized merozoite protein, encoded by gene CSUI_005805, might be a relevant vaccine candidate due to its high immunogenic score, high expression level and species-specificity as determined in silico. METHODS: The 1170 bp coding sequence of the CSUI_005805 gene was PCR amplified and cloned into the bacterial expression vector pQE-31...
February 7, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28169066/standardization-and-validation-of-simple-pcr-duplex-pcr-and-rapd-in-comparison-to-blood-smear-examination-for-diagnosing-bovine-tropical-theileriosis
#11
Vikrant Sudan, Daya Shanker, Amit Jaiswal, Amit Singh, Vijay Pandey
Bovine Tropical Theileriosis (BTT) is an important vector-borne protozoan disease that imposing serious constraints on the health and productivity of domestic cattle. It is matter of common fact that following recovery from primary infection, cattle become persistent carriers and act as reservoirs of infection thereby, playing a critical role in disease epidemiology. The present study describes the comparative diagnostic efficiency of simplex PCR, duplex PCR and RAPD assays for detection of Theileria annulata in cattle...
February 3, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28168382/molecular-design-expression-and-evaluation-of-pasylated-human-recombinant-erythropoietin-with-enhanced-functional-properties
#12
Mohammad Hossein Hedayati, Dariush Norouzian, Mahdi Aminian, Shahram Teimourian, Reza Ahangari Cohan, Soroush Sardari, M Reza Khorramizadeh
Erythropoietin (EPO) is the principal hormone which, has somewhat short half-life involved in the differentiation and regulation of circulating red blood cells. The present study was carried out to evaluate the capability of a polyethylene glycol mimetic technology as a biological alternative to improve pharmaceutical properties of human recombinant EPO. In silico models of EPO fused to 200 amino acids of proline, alanine, and serine (PAS) were initially generated and assessed by molecular dynamic (MD) simulation...
February 2017: Protein Journal
https://www.readbyqxmd.com/read/28167407/efficient-blood-flow-visualization-using-flowline-extraction-and-opacity-modulation-based-on-vascular-structure-analysis
#13
Ohjae Kwon, Jeongjin Lee, Bohyoung Kim, Juneseuk Shin, Yeong-Gil Shin
With the recent advances regarding the acquisition and simulation of blood flow data, blood flow visualization has been widely used in medical imaging for the diagnosis and treatment of pathological vessels. In this paper, we present a novel method for the visualization of the blood flow in vascular structures. The vessel inlet or outlet is first identified using the orthogonality metric between the normal vectors of the flow velocity and vessel surface. Then, seed points are generated on the identified inlet or outlet by Poisson disk sampling...
March 1, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28164534/expression-of-hcv-alternative-reading-frame-protein-core-1-f-in-baculovirus-expression-system-and-its-evaluation-for-assessment-of-specific-anti-core-1-antibody-in-iranian-hcv-infected-patients
#14
Pooneh Rahimi, Rouhollah Vahabpour, Farideh Sadat Sajadian Fard, Fatemeh Motevalli, Fatemeh Fotouhi Chahouki, Mohammad Reza Aghasadeghi, Azam Bolhassani, Seyed Mehdi Sadat, Ehsan Mostafavi, Nasir Mohajel, Ali Jahanian-Najafabadi, Mohammad Reza Amiran
BACKGROUND: Hepatitis C virus (HCV) genome contains an overlapping reading frame which results in alternative core protein (ARFP). Baculovirus expression system was used as a powerful eukaryotic vector system to express core+1/F protein for the first time. This recombinant core+1/F protein was used to assess the anti-core+1 antibody in anti-HCV drug resistant and sustained virologic response (SVR) patients. METHODS: The core+1 coding sequence from HCV genotype 1 was designed and synthesized in pUC57 vector...
October 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28158295/genomic-sequence-of-candidatus-liberibacter-solanacearum-haplotype-c-and-its-comparison-with-haplotype-a-and-b-genomes
#15
Jinhui Wang, Minna Haapalainen, Thomas Schott, Sarah M Thompson, Grant R Smith, Anne I Nissinen, Minna Pirhonen
Haplotypes A and B of 'Candidatus Liberibacter solanacearum' (CLso) are associated with diseases of solanaceous plants, especially Zebra chip disease of potato, and haplotypes C, D and E are associated with symptoms on apiaceous plants. To date, one complete genome of haplotype B and two high quality draft genomes of haplotype A have been obtained for these unculturable bacteria using metagenomics from the psyllid vector Bactericera cockerelli. Here, we present the first genomic sequences obtained for the carrot-associated CLso...
2017: PloS One
https://www.readbyqxmd.com/read/28157153/enhancing-the-biological-relevance-of-machine-learning-classifiers-for-reverse-vaccinology
#16
Ashley I Heinson, Yawwani Gunawardana, Bastiaan Moesker, Carmen C Denman Hume, Elena Vataga, Yper Hall, Elena Stylianou, Helen McShane, Ann Williams, Mahesan Niranjan, Christopher H Woelk
Reverse vaccinology (RV) is a bioinformatics approach that can predict antigens with protective potential from the protein coding genomes of bacterial pathogens for subunit vaccine design. RV has become firmly established following the development of the BEXSERO® vaccine against Neisseria meningitidis serogroup B. RV studies have begun to incorporate machine learning (ML) techniques to distinguish bacterial protective antigens (BPAs) from non-BPAs. This research contributes significantly to the RV field by using permutation analysis to demonstrate that a signal for protective antigens can be curated from published data...
February 1, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28152994/transductive-learning-as-an-alternative-to-translation-initiation-site-identification
#17
Cristiano Lacerda Nunes Pinto, Cristiane Neri Nobre, Luis Enrique Zárate
BACKGROUND: The correct protein coding region identification is an important and latent problem in the molecular biology field. This problem becomes a challenge due to the lack of deep knowledge about the biological systems and unfamiliarity of conservative characteristics in the messenger RNA (mRNA). Therefore, it is fundamental to research for computational methods aiming to help the patterns discovery for identification of the Translation Initiation Sites (TIS). In the field of Bioinformatics, machine learning methods have been widely applied based on the inductive inference, as Inductive Support Vector Machine (ISVM)...
February 2, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28150734/mitochondrial-genome-diversity-in-dagger-and-needle-nematodes-nematoda-longidoridae
#18
J E Palomares-Rius, C Cantalapiedra-Navarrete, A Archidona-Yuste, V C Blok, P Castillo
Dagger and needle nematodes included in the family Longidoridae (viz. Longidorus, Paralongidorus, and Xiphinema) are highly polyphagous plant-parasitic nematodes in wild and cultivated plants and some of them are plant-virus vectors (nepovirus). The mitochondrial (mt) genomes of the dagger and needle nematodes, Xiphinema rivesi, Xiphinema pachtaicum, Longidorus vineacola and Paralongidorus litoralis were sequenced in this study. The four circular mt genomes have an estimated size of 12.6, 12.5, 13.5 and 12...
February 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28139886/genomic-integration-of-the-full-length-dystrophin-coding-sequence-in-duchenne-muscular-dystrophy-induced-pluripotent-stem-cells
#19
Alfonso P Farruggio, Mital S Bhakta, Haley du Bois, Julia Ma, Michele P Calos
We developed plasmid vectors that express the full-length human dystrophin coding sequence in human cells. Dystrophin, the protein mutated in Duchenne muscular dystrophy, is extraordinarily large, providing challenges for cloning and plasmid production in E. coli. We expressed dystrophin from the strong, widely expressed CAG promoter, along with co-transcribed luciferase and mCherry marker genes useful for tracking plasmid expression. Introns were added at the 3' and 5' ends of the dystrophin sequence to prevent translation in E...
January 31, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28137563/evaluating-exosome-protein-content-changes-induced-by-virus-activity-using-silac-labeling-and-lc-ms-ms
#20
X Zhao, Y Xie, J Liu
Exosomes are small membrane vesicles that are produced by cells and excreted into extracellular space. Contents of exosomes generally include lipid, membrane, and soluble proteins, and various types of coding and noncoding RNAs. Over the past decades, it has become clear that exosomes constitute an important vector for intercellular transport and communication with significant functional relevance. Evaluating exosome contents and their changes are vital for understanding its role in different physiological and pathological processes...
2017: Methods in Enzymology
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