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Hypoxia Imaging

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https://www.readbyqxmd.com/read/28727417/oxygen-dependent-lipid-profiles-of-3d-cultured-human-chondrocytes-revealed-by-maldi-msi
#1
Brenda Bakker, Gert B Eijkel, Ron M A Heeren, Marcel Karperien, Janine N Post, Berta Cillero Pastor
Articular cartilage is exposed to a gradient of oxygen levels ranging from 5% at the surface to 1% in the deepest layers. While most cartilage research is performed in supraphysiological oxygen levels (19-21%), culturing chondrocytes under hypoxic oxygen levels (≤ 8%) promotes the chondrogenic phenotype. Exposure of cells to various oxygen levels alters their lipid metabolism, but detailed studies examining how hypoxia affects lipid metabolism in chondrocytes are lacking. To better understand the chondrocyte's behavior in response to oxygen we cultured 3D pellets of human primary chondrocytes in normoxia (20% oxygen) and hypoxia (2...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28720571/the-integrated-stress-response-in-hypoxia-induced-diffuse-white-matter-injury
#2
Benjamin Ll Clayton, Aaron Huang, Rejani B Kunjamma, Ani Solanki, Brian Popko
Currently no treatments exist for preterm infants with diffuse white matter injury (DWMI) caused by hypoxia. Due to improved care of preterm neonates and increased recognition by advanced imaging techniques, the prevalence of DWMI is increasing. A better understanding of the pathophysiology of DWMI is therefore of critical importance. The integrated stress response (ISR), a conserved eukaryotic response to myriad stressors including hypoxia, may play a role in hypoxia-induced DWMI and may represent a novel target for much needed therapies...
July 18, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28718064/contribution-of-circulatory-disturbances-in-subchondral-bone-to-the-pathophysiology-of-osteoarthritis
#3
REVIEW
Roy K Aaron, Jennifer Racine, Jonathan P Dyke
PURPOSE OF REVIEW: This review describes the contributions of abnormal bone circulation to the pathophysiology of osteoarthritis. Combining dynamic imaging with MRI and PET with previous observations reveals that venous stasis and a venous outlet syndrome is most likely the key circulatory pathology associated with the initiation or progression of osteoarthritis. RECENT FINDINGS: MRI and PET have revealed that venous outflow obstruction results in physicochemical changes in subchondral bone to which osteoblasts are responsive...
August 2017: Current Rheumatology Reports
https://www.readbyqxmd.com/read/28714513/in-vivo-evaluation-of-tumour-acidosis-for-assessing-the-early-metabolic-response-and-onset-of-resistance-to-dichloroacetate-by-using-magnetic-resonance-ph-imaging
#4
Annasofia Anemone, Lorena Consolino, Laura Conti, Francesca Reineri, Federica Cavallo, Silvio Aime, Dario Livio Longo
Dichloroacetate (DCA) can reverse the glycolytic phenotype that is responsible of increased lactate production and extracellular pH acidification in cancer cells. Magnetic resonance imaging-chemical exchange saturation transfer (MRI-CEST) pH mapping is a novel non-invasive imaging approach that can measure in vivo extracellular tumour pH. We examined whether MRI-CEST pH mapping can monitor in vivo changes in tumour acidosis for assessing treatment response to DCA. Cell viability and extracellular pH were assessed in TS/A breast cancer cells treated with 1-10 mM DCA for 24 h in normoxia or hypoxia (1% O2) conditions...
August 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28708033/neuropsychological-profile-and-social-cognition-in-congenital-central-hypoventilation-syndrome-cchs-correlation-with-neuroimaging-in-a-clinical-case
#5
Borja Esteso Orduña, Raquel Seijas Gómez, Elena García Esparza, Emily M Briceño, Javier Melero Llorente, María de la Concepción Fournier Del Castillo
INTRODUCTION: Congenital central hypoventilation syndrome (CCHS) is a rare genetic disorder due to paired-like homeobox gene (PHOX2B) mutations. CCHS patients suffer from dysregulation of the autonomic nervous system characterized by the absence of or extremely reduced response to hypercapnia and hypoxia, with neuropsychological deficits. The aim of this exploratory study is to describe the longitudinal neuropsychological profile and its correlations with magnetic resonance imaging (MRI) of a child with CCHS with a PHOX2B mutation...
July 14, 2017: Journal of Clinical and Experimental Neuropsychology
https://www.readbyqxmd.com/read/28705470/hypoxia-and-hypoxia-response-associated-molecular-markers-in-esophageal-cancer-a-systematic-review
#6
REVIEW
Jurgen Peerlings, Lien Van De Voorde, Cristina Mitea, Ruben Larue, Ala Yaromina, Sebastian Sandeleanu, Linda Spiegelberg, Ludwig Dubois, Philippe Lambin, Felix M Mottaghy
PURPOSE: In this systematic review, the existing evidence of available hypoxia-associated molecular response biomarkers in esophageal cancer (EC) patients is summarized and set into the context of the role of hypoxia in the prediction of esophageal cancer, treatment response and treatment outcome. METHODS: A systematic literature search was performed in Web of Science, MEDLINE, and PubMed databases using the keywords: hypoxia, esophagus, cancer, treatment outcome and treatment response...
July 10, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28703233/copper-complexes-with-dissymmetrically-substituted-bis-thiosemicarbazone-ligands-as-a-basis-for-pet-radiopharmaceuticals-control-of-redox-potential-and-lipophilicity
#7
Oliver C Brown, Julia Baguña Torres, Katherine B Holt, Philip J Blower, Michael J Went
Copper(ii) bis(thiosemicarbazone) derivatives have been used extensively in positron emission tomography (PET) to image hypoxia and blood flow and to radiolabel cells for cell tracking. These applications depend on control of redox potentials and lipophilicity of the bis(thiosemicarbazone) complexes, which can be adjusted by altering peripheral ligand substituents. This paper reports the synthesis of a library of new dissymmetrically substituted bis(thiosemicarbazone) ligands by controlling the condensation reactions between dicarbonyl compounds and 4-substituted-3-thiosemicarbazides or using acetal protection...
July 13, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28695498/fmiso-accumulation-in-tumor-is-dependent-on-glutathione-conjugation-capacity-in-addition-to-hypoxic-state
#8
Yukiko Masaki, Yoichi Shimizu, Takeshi Yoshioka, Ken-Ichi Nishijima, Songji Zhao, Kenichi Higashino, Yoshito Numata, Nagara Tamaki, Yuji Kuge
OBJECTIVE: (18)F-fluoromisonidazole (FMISO), a well-known PET imaging probe for diagnosis of hypoxia, is believed to accumulate in hypoxic cells via covalent binding with macromolecules after reduction of the nitro group. Previously, we showed the majority of (18)F-FMISO was incorporated into low-molecular-weight metabolites in hypoxic tumors, and the glutathione conjugate of reduced FMISO (amino-FMISO-GS) distributed in the tumor hypoxic regions as revealed by imaging mass spectrometry (IMS)...
July 10, 2017: Annals of Nuclear Medicine
https://www.readbyqxmd.com/read/28691130/young-and-advanced-tumor-some-2d-electrodynamic-distinctions-melanoma-and-satellite-during-a-vascular-occlusion-test-feasibility-study
#9
Y Babich, M Nuzhdina, S Syniuta
Conventional methods of electrobioimpedance imaging are not suited for adequate visualization of the skin electrical impedance landscape (SEL) because they do not provide high spatial resolution at large enough area of view. The skin electrodynamics introscopy (SEI) enabled dynamic spectral imaging of the SEL at 32 × 64 mm(2) area with 1 mm spatial resolution. The focus of the study was to investigate the SEL distinguishing features between early and advanced-stage cancer at the model object of melanoma and its satellite...
July 10, 2017: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/28690953/venous-diversion-surgery-revisited-a-baffling-situation
#10
Jessica R Klassen, Davinder S Jassal, Brett Memauri, Malek Kass, James W Tam, Jonathan Windram, David Ross, Nasir Shaikh
With the increasing number of survivors with congenital heart disease (CHD) reaching adulthood, it is important for the clinician to be familiar with the various surgical options performed in this growing patient population. We describe the case of a 65-year-old female who presented with hypoxia and right-to-left shunting following a surgical repair of an atrial septal defect (ASD) secundum and anomalous pulmonary veins with a partial atrial diversion procedure in childhood. The use of multimodality cardiovascular imaging using echocardiography, computed tomography, magnetic resonance imaging, and invasive cardiac catheterization was complementary in the preoperative diagnosis and management of this unique baffling situation...
June 6, 2017: Curēus
https://www.readbyqxmd.com/read/28690120/the-prognosis-outcome-of-oral-squamous-cell-carcinoma-using-hif-2%C3%AE
#11
Elva Lim, Chia-Chun Kuo, Hsi-Feng Tu, Cheng-Chieh Yang
BACKGROUND: Hypoxia-induced factors (HIF) has a role in angiogenesis and regulate tumorigenesis of cancer cell. The HIF is the best-identified mechanism that shows imbalance between consumption and oxygen supply in progressing tumor. This study of HIF-2α expression in oral squamous cell carcinoma (OSCC) aimed to investigate the relationship of HIF-2α and pathology characteristics related to its clinical correlation. METHODS: Fifty-eight samples of OSCC and adjacent tissues were fixed in paraffin for microarray preparation...
July 6, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/28685461/correlation-between-hypoxia-proteins-and-epr-detected-hypoxia-in-tumors
#12
Martyna Krzykawska-Serda, Richard C Miller, Martyna Elas, Boris Epel, Eugene D Barth, Mathew Maggio, Howard J Halpern
Rapid expansion of tumor cells that outpace existing vasculature essential for nutrient and oxygen support as well as waste removal, correlates with profound changes in the microenvironment including angiogenesis, vasodilation, glucose metabolism, and cell cycle perturbations. Since hypoxic cells are up to three times more radioresistant than normoxic cells, identification of hypoxic populations to predict radiotherapeutic outcome is important. The consequences of hypoxia and activated proteins contribute to radioresistant tumors and radiotherapeutic failure...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28685439/brain-tissue-po2-measurement-during-normoxia-and-hypoxia-using-two-photon-phosphorescence-lifetime-microscopy
#13
Kui Xu, David A Boas, Sava Sakadžić, Joseph C LaManna
Key to the understanding of the principles of physiological and structural acclimatization to changes in the balance between energy supply (represented by substrate and oxygen delivery, and mitochondrial oxidative phosphorylation) and energy demand (initiated by neuronal activity) is to determine the controlling variables, how they are sensed and the mechanisms initiated to maintain the balance. The mammalian brain depends completely on continuous delivery of oxygen to maintain its function. We hypothesized that tissue oxygen is the primary sensed variable...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28685434/computational-simulation-of-tumor-hypoxia-based-on-in-vivo-microvasculature-assessed-in-a-dorsal-skin-window-chamber
#14
Lina Xu, Peter Vaupel, Siwei Bai, Bjoern Menze, Kuangyu Shi
Malignant growth usually leads to the depletion of oxygen (O2) supply in most solid tumors. Hypoxia can cause resistance to standard radiotherapy, some chemotherapy and immunotherapy. Furthermore, it can also trigger malignant progression by modulating gene expression and inducing genetic instability. The relationship between microvasculature, perfusion and tumor hypoxia has been intensively studied and many computational simulations have been developed to model tissue O2 transport. Usually simplified 2D phantoms are used to investigate tumor hypoxia and it is assumed that vessels are perpendicular to the region of interest and randomly distributed across the domain...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28685433/multiparametric-analysis-of-the-tumor-microenvironment-hypoxia-markers-and-beyond
#15
Arnulf Mayer, Peter Vaupel
We have established a novel in situ protein analysis pipeline, which is built upon highly sensitive, multichannel immunofluorescent staining of paraffin sections of human and xenografted tumor tissue. Specimens are digitized using slide scanners equipped with suitable light sources and fluorescence filter combinations. Resulting digital images are subsequently subjected to quantitative image analysis using a primarily object-based approach, which comprises segmentation of single cells or higher-order structures (e...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28679773/bevacizumab-induces-acute-hypoxia-and-cancer-progression-in-patients-with-refractory-breast-cancer-multimodal-functional-imaging-and-multiplex-cytokine-analysis
#16
Shigeto Ueda, Toshiaki Saeki, Akihiko Osaki, Tomohiko Yamane, Ichiei Kuji
Bevacizumab, an antibody against endothelial growth factor, is a key but controversial drug in the treatment of metastatic breast cancer. We, therefore, aimed to determine the intrinsic resistance to bevacizumab at the physiological and molecular levels in advanced breast cancer using positron emission tomography (PET), dynamic contrast-enhanced MRI, diffuse optical spectroscopic imaging (DOSI), and multiplex cytokine assays.<br />Experimental Design: In total, 28 patients diagnosed with stage III/IV advanced breast cancer receiving single-agent bevacizumab for 1 week followed by paclitaxel combined with bevacizumab underwent (18)F-fluorodeoxyglucose (FDG)-PET, (18)F-fluoromisonidazole (FMISO)-PET, and MRI at both baseline and two courses after treatment initiation...
July 5, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28679680/chronic-hypoxia-alters-maternal-uterine-and-fetal-hemodynamics-in-the-term-pregnant-guinea-pig
#17
Shifa Turan, Graham W Aberdeen, Loren P Thompson
Placental hypoxia is associated with maternal hypertension, placental insufficiency and fetal growth restriction. In the pregnant guinea pig, prenatal hypoxia during early gestation inhibits cytotrophoblast invasion of spiral arteries, increases maternal blood pressure and induces fetal growth restriction. This study evaluated the impact of chronic maternal hypoxia on fetal heart structure with 4D echocardiography with spatio-temporal image correlation and tomographic ultrasound (STIC-TUI) and uterine and umbilical artery resistance/pulsatility indices and fetal heart function using pulsed wave Doppler ultrasound...
July 5, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/28667838/quantitative-ratiometric-phosphorescence-hypoxia-sensing-nanoprobes-based-on-quantum-dots-ir-iii-glycerol-monoolein-cubic-phase-nanoparticles
#18
Jiajun Liu, Youshen Wu, Yao Yu, Ke Li, Yuanyuan Ji, Daocheng Wu
A novel protocol is developed to prepare quantum dot (QD)/Ir(III) complex glycerol monoolein (GMO) cubic-phase nanoparticles (Qd/Ir GMCPNPs) as hypoxia nanoprobes, in which hypoxia probe Tris [1-phenylisoquinoline-C2, N] Iridium(III) [Ir(piq)3] and the reference QDs are separately loaded at hydrophilic and hydrophobic channels to avoid interference. Qd/Ir GMCPNPs were nearly spherical in shape, with an average size of 20-30nm. Their phosphorescence spectra showed that nanoprobes have a wide excited wave length range of 360-500nm, which is suitable for different types of measurement instruments...
June 24, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28667400/microfluidic-device-flow-field-characterization-around-tumor-spheroids-with-tunable-necrosis-produced-in-an-optimized-off-chip-process
#19
James Baye, Casey Galvin, Amy Q Shen
Tumor spheroids are a 3-D tumor model that holds promise for testing cancer therapies in vitro using microfluidic devices. Tailoring the properties of a tumor spheroid is critical for evaluating therapies over a broad range of possible indications. Using human colon cancer cells (HCT-116), we demonstrate controlled tumor spheroid growth rates by varying the number of cells initially seeded into microwell chambers. The presence of a necrotic core in the spheroids could be controlled by changing the glucose concentration of the incubation medium...
September 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28666802/a-novel-modified-physiologically-relevant-model-for-cardiac-angiogenesis
#20
Hao Zhang, Jiajun Pan, Fan Qiu, Tengteng Wei, Zhimin Li, Xia Jiang, Wei Zhuang, Zhiwei Liu, Zhongming Zhang, Hongyan Dong
Angiogenesis assays are important tools for studying both the mechanisms of cardiac angiogenesis and the potential development of therapeutic strategies to ischemic heart diseases. Currently, various assays have been used to quantitate cardiac tubule formation, yet no consensus has been reached regarding a suitable assay for evaluating the efficacy of angiogenic stimulants or inhibitors. Most in vivo angiogenesis assays are complex and difficult to interpret, whereas traditional in vitro angiogenesis models measure only one aspect of this process...
June 28, 2017: Microvascular Research
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