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Hypoxia Imaging

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https://www.readbyqxmd.com/read/28230651/hypoxia-induces-internalization-of-%C3%AE%C2%BA-opioid-receptor
#1
Chunhua Xi, Xuan Liang, Chunhua Chen, Hasan Babazada, Tianzuo Li, Renyu Liu
BACKGROUND: It has been demonstrated that κ-opioid receptor agonists can reduce hypoxia-ischemia brain injury in animal models. However, it is unclear how the κ-opioid receptor responds to hypoxia-ischemia. In the current study, the authors used an in vitro model of oxygen-glucose deprivation and reoxygenation to explore how κ-opioid receptors respond to hypoxia and reoxygenation. METHODS: Mouse neuroblastoma Neuro2A cells were stably transfected with mouse κ-opioid receptor-tdTomato fusion protein or Flag-tagged mouse κ-opioid receptor, divided into several groups (n = 6 to 12), and used to investigate the κ-opioid receptor movement...
February 23, 2017: Anesthesiology
https://www.readbyqxmd.com/read/28226317/neuroprotective-effects-of-acetyl-l-carnitine-on-neonatal-hypoxia-ischemia-induced-brain-injury-in-rats
#2
Shiyu Tang, Su Xu, Xin Lu, Rao P Gullapalli, Mary C McKenna, Jaylyn Waddell
Perinatal hypoxia ischemia (HI) is a significant cause of brain injury in surviving infants. Although hypothermia improves outcomes in some infants, additional therapies are needed since about 40% of infants still have a poor outcome. Acetyl-L-carnitine (ALCAR), an acetylated derivative of L-carnitine, protected against early changes in brain metabolites and mitochondrial function after HI on postnatal day (PND) 7 in a rat pup model of near-term HI injury. However, its efficacy in long-term structural and functional outcomes remains unexplored...
February 23, 2017: Developmental Neuroscience
https://www.readbyqxmd.com/read/28217793/quantitative-analysis-of-the-proliferative-to-invasive-transition-of-hypoxic-glioma-cells
#3
Hector Gomez
Hypoxia is a hallmark of gliomas that is often associated with poor prognosis and resistance to therapies. Insufficient oxygen supply reduces the proliferation rate of tumor cells, which contributes to a slower progression of the lesion, but also increases the invasiveness of the tumor, making it more aggressive. To understand how these two counteracting mechanisms combine and modify the tumor's global growth, this paper proposes a quantitative approach based on a biomathematical model. The model predicts that the net effect of the proliferative-to-invasive transition leads to a lower survival even for slight increments of the invasive capacity of hypoxic tumor cells...
February 20, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/28216300/boronic-acid-tethered-amphiphilic-hyaluronic-acid-derivative-based-nanoassemblies-for-tumor-targeting-and-penetration
#4
Jae Young Jeong, Eun-Hye Hong, Song Yi Lee, Jae-Young Lee, Jae-Hyoung Song, Seung-Hak Ko, Jae-Seong Shim, Sunghwa Choe, Dae-Duk Kim, Hyun-Jeong Ko, Hyun-Jong Cho
(3-Aminomethylphenyl)boronic acid (AMPB)-installed hyaluronic acid-ceramide (HACE)-based nanoparticles (NPs), including manassantin B (MB), were fabricated for tumor-targeted delivery. The amine group of AMPB was conjugated to the carboxylic acid group of hyaluronic acid (HA) via amide bond formation, and synthesis was confirmed by spectroscopic methods. HACE-AMPB/MB NPs with a 239-nm mean diameter, narrow size distribution, negative zeta potential, and > 90% drug encapsulation efficiency were fabricated...
February 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28215133/use-of-imaging-studies-for-determination-of-brain-death-in-south-australian-intensive-care-units
#5
Brett G Sampson, Luke D Datson, Shailesh Bihari
OBJECTIVES: To describe the use of imaging studies (four-vessel angiography or radionuclide scan) for brain death determination in South Australian intensive care units, and to determine the rates of adherence with The ANZICS statement on death and organ donation of the Australian and New Zealand Intensive Care Society (ANZICS). DESIGN, PATIENTS AND SETTING: Retrospective case-note review of 190 South Australian adult patients (≥ 18 years) who were brain dead and were organ donors (actual and intended), from 1 January 2008 to 31 December 2014...
March 2017: Critical Care and Resuscitation: Journal of the Australasian Academy of Critical Care Medicine
https://www.readbyqxmd.com/read/28212880/automatic-detection-of-hypoxia-in-renal-tissue-stained-with-hif-alpha
#6
Aline Rodrigues Buzin, Nayana Damiani Macedo, Isabela Bastos Binotti Abreu De Araujo, Breno Valentim Nogueira, Tadeu Uggere De Andrade, Denise Coutinho Endringer, Dominik Lenz
OBJECTIVE: The objective of this study was the identification of the stain HIF-alpha using the Image Cytometry, and to help to count the positive cells (with HIF-alpha) and the negative cells (without HIF-alpha) from the same sample. METHOD: 17 images of renal tissues from male rats of Winstar lineage; overall, there were 12.587 objects (cells) in the images for analysis. The acquired images were then analyzed through the free softwares CellProfiler (version 2.1...
February 14, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28210996/quantitative-evaluation-of-oxygen-metabolism-in-the-intratumoral-hypoxia-18-f-fluoromisonidazole-and-15-o-labelled-gases-inhalation-pet
#7
Tadashi Watabe, Yasukazu Kanai, Hayato Ikeda, Genki Horitsugi, Keiko Matsunaga, Hiroki Kato, Kayako Isohashi, Kohji Abe, Eku Shimosegawa, Jun Hatazawa
BACKGROUND: Intratumoral hypoxia is one of the resistant factors in radiotherapy and chemotherapy for cancer. Although it is detected by (18)F-fluoromisonidazole (FMISO) PET, the relationship between intratumoral hypoxia and oxygen metabolism has not been studied. The purpose of this study was to evaluate the intratumoral perfusion and oxygen metabolism in hypoxic regions using the rat xenograft model. Ten male Fischer rats with C6 glioma (body weight = 220 ± 15 g) were investigated with (18)F-FMISO PET and steady-state inhalation method of (15)O-labelled gases PET...
December 2017: EJNMMI Research
https://www.readbyqxmd.com/read/28209906/first-evaluation-of-pet-based-human-biodistribution-and-dosimetry-of-18-f-faza-a-tracer-for-imaging-tumor-hypoxia
#8
Savi Annarita, Elena Incerti, Federico Fallanca, Valentino Bettinardi, Francesca Rossetti, Cristina Monterisi, Antonia Compierchio, Giampiero Negri, Piero Zannini, Luigi Gianolli, Maria Picchio
Fluorine-18 labelled fluoroazomycinarabinoside ((18)F-FAZA) is a positron emission tomography (PET) biomarker for non-invasive identification of regional tumor hypoxia. Aim of the present Phase I study was to firstly evaluate in non-small cell lung cancer patients the human biodistribution and dosimetry of (18)F-FAZA. Methods: Five patients awaiting surgical resection after histologically proven or radiologically suspected non-small cell lung cancer were prospectively enrolled for the study. The patients underwent a PET/computed tomography (CT) study after the injection of 371±32 MBq of (18)F-FAZA...
February 16, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28203097/cerebral-malaria-insight-into-pathogenesis-complications-and-molecular-biomarkers
#9
Farah Hafiz Yusuf, Muhammad Yusuf Hafiz, Maria Shoaib, Syed Ahsanuddin Ahmed
Cerebral malaria is a medical emergency. All patients with Plasmodium falciparum malaria with neurologic manifestations of any degree should be urgently treated as cases of cerebral malaria. Pathogenesis of cerebral malaria is due to damaged vascular endothelium by parasite sequestration, inflammatory cytokine production and vascular leakage, which result in brain hypoxia, as indicated by increased lactate and alanine concentrations. The levels of the biomarkers' histidine-rich protein II, angiopoietin-Tie-2 system and plasma osteoprotegrin serve as diagnostic and prognostic markers...
2017: Infection and Drug Resistance
https://www.readbyqxmd.com/read/28202707/cadasil-accelerated-by-acute-hypotension-arterial-and-venous-contribution-to-leukoaraiosis
#10
Jacqueline A Pettersen, Julia Keith, Fuqiang Gao, J David Spence, Sandra E Black
OBJECTIVE: To underline the importance of blood pressure regulation in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) and to describe changes that occur in the veins in this condition, specifically venous collagenosis associated with leukoaraiosis. METHODS: Case report with neuroimaging and pathologic data. RESULTS: A 61-year-old man with genetically confirmed CADASIL was initially lucid following a motor vehicle accident but subsequently became hypotensive (60/40 mm Hg) due to an open femur fracture and required intubation...
February 15, 2017: Neurology
https://www.readbyqxmd.com/read/28197395/characterization-of-the-tumor-microenvironment-and-tumor-stroma-interaction-by-non-invasive-preclinical-imaging
#11
REVIEW
Nirilanto Ramamonjisoa, Ellen Ackerstaff
Tumors are often characterized by hypoxia, vascular abnormalities, low extracellular pH, increased interstitial fluid pressure, altered choline-phospholipid metabolism, and aerobic glycolysis (Warburg effect). The impact of these tumor characteristics has been investigated extensively in the context of tumor development, progression, and treatment response, resulting in a number of non-invasive imaging biomarkers. More recent evidence suggests that cancer cells undergo metabolic reprograming, beyond aerobic glycolysis, in the course of tumor development and progression...
2017: Frontiers in Oncology
https://www.readbyqxmd.com/read/28195439/noninvasive-imaging-of-brain-oxygen-metabolism-in-children-with-primary-nocturnal-enuresis-during-natural-sleep
#12
Bing Yu, Mingzhu Huang, Xu Zhang, Hongwei Ma, Miao Peng, Qiyong Guo
A series of studies have revealed that nocturnal enuresis is closely related to hypoxia in children with primary nocturnal enuresis (PNE). However, brain oxygen metabolism of PNE children has not been investigated before. The purpose of this study was to investigate changes in whole-brain cerebral metabolic rate of oxygen (CMRO2 ), cerebral blood flow (CBF), and oxygen extraction fraction (OEF) in children suffering from PNE. We used the newly developed T2-relaxation-under-spin-tagging (TRUST) magnetic resonance imaging technique...
February 14, 2017: Human Brain Mapping
https://www.readbyqxmd.com/read/28194632/characterization-of-an-orthotopic-colorectal-cancer-mouse-model-and-its-feasibility-for-accurate-quantification-in-positron-emission-tomography
#13
Sara Rapic, Christel Vangestel, Jeroen Verhaeghe, Tim Van den Wyngaert, Rukun Hinz, Marleen Verhoye, Patrick Pauwels, Steven Staelens, Sigrid Stroobants
PURPOSE: Quantification in positron emission tomography (PET) imaging of an orthotopic mouse model of colorectal cancer (CRC) is challenging due to difficult tumor delineation. We aimed to establish a reproducible delineation approach, evaluate its feasibility for reliable PET quantification and compare its added translational value with its subcutaneous counterpart. PROCEDURES: A subcutaneous Colo205-luc2 tumor fragment harvested from a donor mouse was transplanted onto the caecum of nude mice, with (n = 10) or without (n = 10) the addition of an X-ray detectable thread...
February 13, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28186661/brain-neurochemical-and-hemodynamic-findings-in-the-ny1dd-mouse-model-of-mild-sickle-cell-disease
#14
Min-Hui Cui, Sandra M Suzuka, Nicholas A Branch, Kamalakar Ambadipudi, Sangeetha Thangaswamy, Seetharama A Acharya, Henny H Billett, Craig A Branch
To characterize the cerebral profile associated with sickle cell disease (SCD), we used in vivo proton MRI and MRS to quantify hemodynamics and neurochemicals in the thalamus of NY1DD mice, a mild model of SCD, and compared them with wild-type (WT) control mice. Compared with WT mice, NY1DD mice at steady state had elevated cerebral blood flow (CBF) and concentrations of N-acetylaspartate (NAA), glutamate (Glu), alanine, total creatine and N-acetylaspartylglutamate. Concentrations of glutathione (GSH) at steady state showed a negative correlation with BOLD signal change in response to 100% oxygen, a marker for oxidative stress, and mean diffusivity assessed using diffusion-tensor imaging, a marker for edematous inflammation...
February 10, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28185197/multiplex-immunohistochemistry-for-mapping-the-tumor-microenvironment
#15
Jessica Kalra, Jennifer Baker
Multiplex Immunohistochemistry (mIHC) allows for the simultaneous detection of multiple targets of interest in a single tissue section. Using this method it is therefore possible to evaluate several effectors of a signal transduction pathway at one time, co-localization of markers, or distribution of molecules relative to one another within tissues. This method plays an important role in characterizing molecular signaling and the outcome of signal ablation when assessing mechanisms of drug action. Additionally, this approach can be used to characterize: the tumor microenvironment including vascular architecture and hypoxia, cellular proliferation, cell death as well as drug distribution...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28183993/multiparametric-imaging-of-tumor-hypoxia-and-perfusion-with-18-f-fmiso-dynamic-pet-in-head-and-neck-cancer
#16
Milan Grkovski, Heiko Schöder, Nancy Lee, Sean D Carlin, Bradley Jay Beattie, Nadeem Riaz, Jonathan E Leeman, Joseph A O'Donoghue, John Laurence Humm
Tumor hypoxia and perfusion are independent prognostic indicators of patient outcome. We developed the methodology for, and investigated the utility of multiparametric imaging of tumor hypoxia and perfusion with (18)F-fluoromisonidazole (FMISO) dynamic positron emission tomography (dPET) in head and neck cancer (HNC). Methods: 120 HNC patients underwent 0-30 min FMISO dPET in a customized immobilization mask, followed by 10 min static acquisitions starting at 93±6 and 160±13 min post-injection. A total of 248 lesions (≥2 cm(3)) were analyzed...
February 9, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28183987/in-vivo-hypoxia-pet-imaging-quantifies-the-severity-of-arthritic-joint-inflammation-in-line-with-overexpression-of-hif-and-enhanced-ros-generation
#17
Kerstin Fuchs, Anna Kuehn, Moritz Mahling, Philipp Guenthoer, Andreas Hector, Dominik Hartl, Stefan Laufer, Ursula Kohlhofer, Leticia Quintanilla-Martinez, Gerald Reischl, Martin Röcken, Bernd J Pichler, Manfred Kneilling
Hypoxia is essential for the development of autoimmune diseases such as rheumatoid arthritis (RA) and is associated with the expression of reactive oxygen species (ROS), due to enhanced infiltration of immune cells. The aim of this study was to demonstrate the feasibility of measuring hypoxia non-invasively in vivo in arthritic ankles with positron emission tomography (PET)/magnetic resonance imaging (MRI) using the hypoxia tracers (18)F-fluoromisonidazole (FMISO) and (18)F-fluoroazomycinarabinoside (FAZA)...
February 9, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28174322/4d-microvascular-analysis-reveals-that-regenerative-angiogenesis-in-ischemic-muscle-produces-a-flawed-microcirculation
#18
John-Michael Arpino, Zengxuan Nong, Fuyan Li, Hao Yin, Nour W Ghonaim, Stephanie Milkovich, Brittany Balint, Caroline O'Neil, Graham M Fraser, Daniel Goldman, Christopher G Ellis, J G Pickering
RATIONALE: Angiogenesis occurs following ischemic injury to skeletal muscle and enhancing this response has been a therapeutic goal. However, to appropriately deliver oxygen, a precisely organized and exquisitely responsive microcirculation must form. Whether these network attributes exist in a regenerated microcirculation is unknown and methodologies for answering this have been lacking. OBJECTIVE: To develop 4D methodologies for elucidating microarchitecture and function of the reconstructed microcirculation in skeletal muscle...
February 7, 2017: Circulation Research
https://www.readbyqxmd.com/read/28170538/microglia-activation-and-recruitment-of-circulating-macrophages-during-ischemic-experimental-branch-retinal-vein-occlusion
#19
Andreas Ebneter, Despina Kokona, Nadia Schneider, Martin S Zinkernagel
Purpose: To characterize retinal microglia activation and macrophage recruitment in experimental branch retinal vein occlusion (BRVO). Methods: Experimental BRVO was induced in Balb/c mice and histologic changes were studied. Tissue hypoxia was visualized using pimonidazole hydrochloride. Monocyte-derived retinal cells were quantified using histology and flow cytometry. To investigate the dynamics of invading blood-borne macrophages, chimera mice were generated using bone marrow grafts from Cx3cr1(gfp/gfp) mice to rescue lethally irradiated wild-type BALB/c mice...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28168986/gastric-emptying-is-reduced-in-experimental-nec-and-correlates-with-the-severity-of-intestinal-damage
#20
Yuhki Koike, Bo Li, Carol Lee, Shigang Cheng, Hiromu Miyake, Christopher Welsh, Alison Hock, Jaques Belik, Augusto Zani, Agostino Pierro
PURPOSE: The aim of this study is to assess gastric emptying in experimental necrotizing enterocolitis (NEC) and its diagnostic significance using non-invasive ultrasound imaging. METHODS: Fourteen neonatal mice (C57BL/6) were randomized into two groups: NEC [n=10] and control [n=4]. NEC was induced by gavage feeding of hyperosmolar formula, hypoxia, and lipopolysaccharide between postnatal day 5 (P5) and 9 (P9). Stomach volume was measured using a 40-MHz ultrasound transducer on P5 and P9...
January 29, 2017: Journal of Pediatric Surgery
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